Restriction map for
pFN21AA1646
(Circular) (Six-base) MAPSORT of: pFN21AA1646.seq Check: 5379 from: 1 to: 6316
pFN21AA1646 6316 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2296 2296
Size: 6316
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2387 4126 278
Size: 53 83 186 936 851 1739 2468
Fragments arranged by size:
2468 1739 936 851 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2655 3659 1937
Size: 718 1004 4594
Fragments arranged by size:
4594 1004 718
Acc65I G'GTAC_C
Cuts at: 3637 3637
Size: 6316
AclI AA'CG_TT
Cuts at: 1563 4443 4816 1563
Size: 2880 373 3063
Fragments arranged by size:
3063 2880 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4379 5763 6031 1632
Size: 2747 1384 268 1917
Fragments arranged by size:
2747 1917 1384 268
AfeI AGC'GCT
Cuts at: 2063 3440 2063
Size: 1377 4939
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6119
AflIII A'CryG_T
Cuts at: 1203 3189 5215 6192 1203
Size: 1986 2026 977 1327
Fragments arranged by size:
2026 1986 1327 977
AgeI A'CCGG_T
Cuts at: 5147 5147
Size: 6316
AhdI GACnn_n'nnGTC
Cuts at: 5045 5045
Size: 6316
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6316
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3546 1184
Size: 2362 3954
AlwNI CAG_nnn'CTG
Cuts at: 2359 3085 3498 4059 5631 2359
Size: 726 413 561 1572 3044
Fragments arranged by size:
3044 1572 726 561 413
ApaI G_GGCC'C
Cuts at: 2124 2124
Size: 6316
ApaLI G'TGCA_C
Cuts at: 4373 5529 4373
Size: 1156 5160
ApoI r'AATT_y
Cuts at: 1493 3374 3625 3851 4003 6112 1493
Size: 1881 251 226 152 2109 1697
Fragments arranged by size:
2109 1881 1697 251 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4870 160
Size: 872 3838 1606
Fragments arranged by size:
3838 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6316
AvaI C'yCGr_G
Cuts at: 1870 1943 2116 2670 3641 1870
Size: 73 173 554 971 4545
Fragments arranged by size:
4545 971 554 173 73
BamHI G'GATC_C
Cuts at: 3646 4013 3646
Size: 367 5949
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2132 2286 2467 2753 3637
Size: 325 217 627 345 154 181 286 884
Cuts at: 3637 5092 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 884 627 345 325 286 217
181 154
BanII G_rGCy'C
Cuts at: 1391 2085 2095 2117 2124 3215 3635 1391
Size: 694 10 22 7 1091 420 4072
Fragments arranged by size:
4072 1091 694 420 22 10 7
BbeI G_GCGC'C
Cuts at: 2136 2136
Size: 6316
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3246 928
Size: 971 1347 3998
Fragments arranged by size:
3998 1347 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2754 2788 4507 4541 1903
Size: 34 817 34 1719 34 3678
Fragments arranged by size:
3678 1719 817 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4208 5418 4208
Size: 1210 5106
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6316
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3219 4927
Size: 107 122 71 631 605 337 1210 1708
Cuts at: 4927 136
Size: 1525
Fragments arranged by size:
1708 1525 1210 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 6311 6311
Size: 6316
BlpI GC'TnA_GC
Cuts at: 3733 3733
Size: 6316
Bme1580I G_kGCm'C
Cuts at: 1790 2124 2756 3450 4377 5533 1790
Size: 334 632 694 927 1156 2573
Fragments arranged by size:
2573 1156 927 694 632 334
BmgBI CAC'GTC
Cuts at: 1318 3136 1318
Size: 1818 4498
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2682 4047 5005
Size: 416 53 83 399 24 1265 1365 958
Cuts at: 5005 442
Size: 1753
Fragments arranged by size:
1753 1365 1265 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6316
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2099 2393 3107 3268 4976 1239
Size: 860 294 714 161 1708 2579
Fragments arranged by size:
2579 1708 860 714 294 161
Bpu10I CC'TnA_GC
Cuts at: 2482 2482
Size: 6316
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3404 4441 5306 5604 3404
Size: 1037 865 298 4116
Fragments arranged by size:
4116 1037 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4979 882
Size: 617 3480 2219
Fragments arranged by size:
3480 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4107 493
Size: 711 2903 2702
Fragments arranged by size:
2903 2702 711
BsaBI GATnn'nnATC
Cuts at: 2705 6310 2705
Size: 3605 2711
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2133 2384 4123 4505
Size: 53 83 186 936 600 251 1739 382
Cuts at: 4505 5140 275
Size: 635 1451
Fragments arranged by size:
1739 1451 936 635 600 382 251 186
83 53
BsaWI w'CCGG_w
Cuts at: 2697 3273 4749 5147 5421 5568 5979 2697
Size: 576 1476 398 274 147 411 3034
Fragments arranged by size:
3034 1476 576 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2321 2351 2528 2558 1178
Size: 30 165 30 918 30 177 30 4936
Fragments arranged by size:
4936 918 177 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2342 2656 3104 3107 3155 1186
Size: 1156 314 448 3 48 4347
Fragments arranged by size:
4347 1156 448 314 48 3
BseYI C'CCAG_C
Cuts at: 2866 5519 6015 2866
Size: 2653 496 3167
Fragments arranged by size:
3167 2653 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2600 2988 3463 3506 1163
Size: 573 864 388 475 43 3973
Fragments arranged by size:
3973 864 573 475 388 43
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3766 4527 5555 1540
Size: 275 186 1765 761 1028 2301
Fragments arranged by size:
2301 1765 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2281 4377 4462 5533 2281
Size: 2096 85 1071 3064
Fragments arranged by size:
3064 2096 1071 85
BsmI GAATG_Cn'
Cuts at: 3827 3920 3827
Size: 93 6223
BsmBI CGTCTCn'nnnn_
Cuts at: 3240 3490 3240
Size: 250 6066
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2085 2095 2117 2124 2281 2756 3215
Size: 399 295 10 22 7 157 475 459
Cuts at: 3215 3450 3635 4377 4462 5533 1391
Size: 235 185 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 475 459 399 295 235
185 157 85 22 10 7
BspEI T'CCGG_A
Cuts at: 2697 3273 5979 2697
Size: 576 2706 3034
Fragments arranged by size:
3034 2706 576
BspHI T'CATG_A
Cuts at: 4205 5935 4205
Size: 1730 4586
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2296 3671 844
Size: 1452 1375 3489
Fragments arranged by size:
3489 1452 1375
BsrBI CCG'CTC
Cuts at: 1394 1823 4203 1394
Size: 429 2380 3507
Fragments arranged by size:
3507 2380 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4811 4985 65
Size: 1183 3563 174 1396
Fragments arranged by size:
3563 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2129 4960 5147 1700
Size: 110 319 2831 187 2869
Fragments arranged by size:
2869 2831 319 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6316
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6316
BssSI C'ACGA_G
Cuts at: 3576 4376 5388 3576
Size: 800 1012 4504
Fragments arranged by size:
4504 1012 800
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2041 2359 794
Size: 1247 318 4751
Fragments arranged by size:
4751 1247 318
BstBI TT'CG_AA
Cuts at: 5120 5120
Size: 6316
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6316
BstYI r'GATC_y
Cuts at: 1972 3020 3646 4013 4398 4415 5867 5953 5965
Size: 1048 626 367 385 17 1452 86 12
Cuts at: 5965 6311 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1048 626 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2748 2808 513
Size: 552 939 744 60 4021
Fragments arranged by size:
4021 939 744 552 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2161 487
Size: 683 56 935 4642
Fragments arranged by size:
4642 935 683 56
BtsI GCAGTG_nn'
Cuts at: 2224 2971 3550 3832 4626 4646 2224
Size: 747 579 282 794 20 3894
Fragments arranged by size:
3894 794 747 579 282 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6316
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6281
DraI TTT'AAA
Cuts at: 2952 3620 2952
Size: 668 5648
DraIII CAC_nnn'GTG
Cuts at: 3016 3016
Size: 6316
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2576 3515 5323 809
Size: 507 1260 939 1808 1802
Fragments arranged by size:
1808 1802 1260 939 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2805 3171 3763
Size: 54 1425 50 275 18 975 366 592
Cuts at: 3763 4652 6281 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 975 889 592 366 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3763 1537
Size: 275 1951 4090
Fragments arranged by size:
4090 1951 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3582 4246 1353
Size: 2229 664 3423
Fragments arranged by size:
3423 2229 664
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2039 2125 2604 4903 5277 5423
Size: 1064 138 450 86 479 2299 374 146
Cuts at: 5423 387
Size: 1280
Fragments arranged by size:
2299 1280 1064 479 450 374 146 138
86
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2099 2393 3107 3268 4379 4976 5763
Size: 393 467 294 714 161 1111 597 787
Cuts at: 5763 6031 1239
Size: 268 1524
Fragments arranged by size:
1524 1111 787 714 597 467 393 294
268 161
EcoNI CCTnn'n_nnAGG
Cuts at: 3422 3422
Size: 6316
EcoO109I rG'GnC_Cy
Cuts at: 2120 3220 3517 2120
Size: 1100 297 4919
Fragments arranged by size:
4919 1100 297
EcoRI G'AATT_C
Cuts at: 3625 4003 3625
Size: 378 5938
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3457 3457
Size: 6316
FspI TGC'GCA
Cuts at: 4822 4822
Size: 6316
HaeII r_GCGC'y
Cuts at: 1678 2065 2136 2649 2780 3442 5463 1678
Size: 387 71 513 131 662 2021 2531
Fragments arranged by size:
2531 2021 662 513 387 131 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2138 2170 2804 2836 3294
Size: 32 135 32 566 32 634 32 458
Cuts at: 3294 3326 4957 4989 5031 5063 1373
Size: 32 1631 32 42 32 2626
Fragments arranged by size:
2626 1631 634 566 458 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2656 2724 3660 3906 669
Size: 988 281 718 68 936 246 3079
Fragments arranged by size:
3079 988 936 718 281 246 68
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6316
HpaI GTT'AAC
Cuts at: 3906 3906
Size: 6316
KasI G'GCGC_C
Cuts at: 2132 2132
Size: 6316
KpnI G_GTAC'C
Cuts at: 3641 3641
Size: 6316
MfeI C'AATT_G
Cuts at: 3915 3915
Size: 6316
MluI A'CGCG_T
Cuts at: 6192 6192
Size: 6316
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3513 5430 5614 1305
Size: 2208 1917 184 2007
Fragments arranged by size:
2208 2007 1917 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2807 3173 10
Size: 54 1425 343 975 366 3153
Fragments arranged by size:
3153 1425 975 366 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2813 2976 3188 4274 4633 4792 518
Size: 648 1647 163 212 1086 359 159 2042
Fragments arranged by size:
2042 1647 1086 648 359 212 163 159
MspA1I CmG'CkG
Cuts at: 1606 2032 2292 2335 3085 3559 3732 4409 5557
Size: 426 260 43 750 474 173 677 1148
Cuts at: 5557 5802 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 750 677 474 426 260 245
173 43
NaeI GCC'GGC
Cuts at: 2131 2131
Size: 6316
NarI GG'CG_CC
Cuts at: 2133 2133
Size: 6316
NcoI C'CATG_G
Cuts at: 513 1065 2004 2808 513
Size: 552 939 804 4021
Fragments arranged by size:
4021 939 804 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6316
NgoMIV G'CCGG_C
Cuts at: 2129 2129
Size: 6316
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6316
NotI GC'GGCC_GC
Cuts at: 3763 3763
Size: 6316
NspI r_CATG'y
Cuts at: 1132 1229 2522 3193 3674 5219 1132
Size: 97 1293 671 481 1545 2229
Fragments arranged by size:
2229 1545 1293 671 481 97
PasI CC'CwG_GG
Cuts at: 1348 3216 1348
Size: 1868 4448
PciI A'CATG_T
Cuts at: 3189 5215 3189
Size: 2026 4290
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6316
PfoI T'CCnGG_A
Cuts at: 3497 3497
Size: 6316
PmeI GTTT'AAAC
Cuts at: 3620 3620
Size: 6316
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4385 4417 5898 5930 4385
Size: 32 1481 32 4771
Fragments arranged by size:
4771 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3517 3517
Size: 6316
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6316
PsiI TTA'TAA
Cuts at: 3886 3886
Size: 6316
PspOMI G'GGCC_C
Cuts at: 2120 2120
Size: 6316
PstI C_TGCA'G
Cuts at: 830 2355 3668 830
Size: 1525 1313 3478
Fragments arranged by size:
3478 1525 1313
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6316
PvuII CAG'CTG
Cuts at: 2335 3085 3559 2335
Size: 750 474 5092
Fragments arranged by size:
5092 750 474
SalI G'TCGA_C
Cuts at: 1936 2654 3658 1936
Size: 718 1004 4594
Fragments arranged by size:
4594 1004 718
SbfI CC_TGCA'GG
Cuts at: 3668 3668
Size: 6316
ScaI AGT'ACT
Cuts at: 4564 6276 4564
Size: 1712 4604
SfcI C'TryA_G
Cuts at: 826 1045 2351 3664 4799 5480 5671 826
Size: 219 1306 1313 1135 681 191 1471
Fragments arranged by size:
1471 1313 1306 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2134 2134
Size: 6316
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6316
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6316
SmaI CCC'GGG
Cuts at: 1872 2118 2672 3643 1872
Size: 246 554 971 4545
Fragments arranged by size:
4545 971 554 246
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3419 4420 5321 5583 820
Size: 197 926 1476 1001 901 262 1553
Fragments arranged by size:
1553 1476 1001 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4107 493
Size: 3614 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6316
SphI G_CATG'C
Cuts at: 1132 3674 1132
Size: 2542 3774
SspI AAT'ATT
Cuts at: 5 52 4240 5
Size: 47 4188 2081
Fragments arranged by size:
4188 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6316
StyI C'CwwG_G
Cuts at: 513 1065 2004 2532 2808 3026 3755 513
Size: 552 939 528 276 218 729 3074
Fragments arranged by size:
3074 939 729 552 528 276 218
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2038 4343 4360 4513 4698 5143 325
Size: 1255 458 2305 17 153 185 445 1498
Fragments arranged by size:
2305 1498 1255 458 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4562 6274 96
Size: 274 80 33 51 1443 2585 1712 138
Fragments arranged by size:
2585 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2321 2353 2720 2752 6276 6308 2321
Size: 32 367 32 3524 32 2329
Fragments arranged by size:
3524 2329 367 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2989 1316
Size: 1673 4643
XbaI T'CTAG_A
Cuts at: 3652 3652
Size: 6316
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6316
XmaI C'CCGG_G
Cuts at: 1870 2116 2670 3641 1870
Size: 246 554 971 4545
Fragments arranged by size:
4545 971 554 246
XmnI GAAnn'nnTTC
Cuts at: 4445 4445
Size: 6316
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2385 4124 276
Size: 53 83 186 936 851 1739 2468
Fragments arranged by size:
2468 1739 936 851 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI
BstXI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI NruI
NsiI PacI PmlI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SrfI SwaI XcmI