Restriction map for
pFN21AA1645
(Circular) (Six-base) MAPSORT of: pFN21AA1645.seq Check: 4925 from: 1 to: 5686
pFN21AA1645 5686 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2725 2725
Size: 5686
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2453 2589 3496 278
Size: 53 83 186 936 917 136 907 2468
Fragments arranged by size:
2468 936 917 907 186 136 83 53
AccI GT'mk_AC
Cuts at: 1937 3029 1937
Size: 1092 4594
Acc65I G'GTAC_C
Cuts at: 3007 3007
Size: 5686
AclI AA'CG_TT
Cuts at: 1563 3813 4186 1563
Size: 2250 373 3063
Fragments arranged by size:
3063 2250 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2294 2486 3749 5133 5401 1632
Size: 662 192 1263 1384 268 1917
Fragments arranged by size:
1917 1384 1263 662 268 192
AfeI AGC'GCT
Cuts at: 2311 2311
Size: 5686
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5489
AflIII A'CryG_T
Cuts at: 1203 4585 5562 1203
Size: 3382 977 1327
Fragments arranged by size:
3382 1327 977
AgeI A'CCGG_T
Cuts at: 2068 4517 2068
Size: 2449 3237
AhdI GACnn_n'nnGTC
Cuts at: 4415 4415
Size: 5686
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5686
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5686
AlwNI CAG_nnn'CTG
Cuts at: 2233 2431 2731 2866 2964 3429 5001 2233
Size: 198 300 135 98 465 1572 2918
Fragments arranged by size:
2918 1572 465 300 198 135 98
ApaLI G'TGCA_C
Cuts at: 3743 4899 3743
Size: 1156 4530
ApoI r'AATT_y
Cuts at: 1493 2995 3221 3373 5482 1493
Size: 1502 226 152 2109 1697
Fragments arranged by size:
2109 1697 1502 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4240 160
Size: 872 3208 1606
Fragments arranged by size:
3208 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5686
AvaI C'yCGr_G
Cuts at: 1870 1943 2765 3011 1870
Size: 73 822 246 4545
Fragments arranged by size:
4545 822 246 73
BamHI G'GATC_C
Cuts at: 3016 3383 3016
Size: 367 5319
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2057 2112 2244 3007 4462
Size: 325 217 627 270 55 132 763 1455
Cuts at: 4462 618
Size: 1842
Fragments arranged by size:
1842 1455 763 627 325 270 217 132
55
BanII G_rGCy'C
Cuts at: 1391 2101 2634 2942 3005 1391
Size: 710 533 308 63 4072
Fragments arranged by size:
4072 710 533 308 63
BbeI G_GCGC'C
Cuts at: 2116 2248 2116
Size: 132 5554
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4715
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2596 2630 3877 3911 1903
Size: 34 659 34 1247 34 3678
Fragments arranged by size:
3678 1247 659 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3578 4788 3578
Size: 1210 4476
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5686
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2205 2242
Size: 107 122 71 631 605 337 196 37
Cuts at: 2242 2876 4297 136
Size: 634 1421 1525
Fragments arranged by size:
1525 1421 634 631 605 337 196 122
107 71 37
BglII A'GATC_T
Cuts at: 5681 5681
Size: 5686
BlpI GC'TnA_GC
Cuts at: 3103 3103
Size: 5686
Bme1580I G_kGCm'C
Cuts at: 1790 3747 4903 1790
Size: 1957 1156 2573
Fragments arranged by size:
2573 1957 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5686
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2035 2056 3417
Size: 416 53 83 399 24 618 21 1361
Cuts at: 3417 4375 442
Size: 958 1753
Fragments arranged by size:
1753 1361 958 618 416 399 83 53
24 21
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5686
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2458 2458
Size: 5686
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2016 2179 2453 2728 4346 1239
Size: 777 163 274 275 1618 2579
Fragments arranged by size:
2579 1618 777 275 274 163
Bpu10I CC'TnA_GC
Cuts at: 2106 2106
Size: 5686
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3811 4676 4974 3811
Size: 865 298 4523
Fragments arranged by size:
4523 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2139 4349 882
Size: 617 640 2210 2219
Fragments arranged by size:
2219 2210 640 617
BsaAI yAC'GTr
Cuts at: 493 1204 3477 493
Size: 711 2273 2702
Fragments arranged by size:
2702 2273 711
BsaBI GATnn'nnATC
Cuts at: 5680 5680
Size: 5686
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2113 2245 2450 2586
Size: 53 83 186 936 580 132 205 136
Cuts at: 2586 3493 3875 4510 275
Size: 907 382 635 1451
Fragments arranged by size:
1451 936 907 635 580 382 205 186
136 132 83 53
BsaWI w'CCGG_w
Cuts at: 2068 4119 4517 4791 4938 5349 2068
Size: 2051 398 274 147 411 2405
Fragments arranged by size:
2405 2051 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2516 2546 2639 2669 1178
Size: 30 165 30 1113 30 93 30 4195
Fragments arranged by size:
4195 1113 165 93 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2111 2243 2642 1186
Size: 925 132 399 4230
Fragments arranged by size:
4230 925 399 132
BseYI C'CCAG_C
Cuts at: 2488 2820 4889 5385 2488
Size: 332 2069 496 2789
Fragments arranged by size:
2789 2069 496 332
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2066 2626 1163
Size: 573 330 560 4223
Fragments arranged by size:
4223 573 560 330
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3136 3897 4925 1540
Size: 275 186 1135 761 1028 2301
Fragments arranged by size:
2301 1135 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3747 3832 4903 3747
Size: 85 1071 4530
Fragments arranged by size:
4530 1071 85
BsmI GAATG_Cn'
Cuts at: 3197 3290 3197
Size: 93 5593
BsmBI CGTCTCn'nnnn_
Cuts at: 2593 2593
Size: 5686
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2101 2634 2942 3005 3747 3832 4903
Size: 399 311 533 308 63 742 85 1071
Cuts at: 4903 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 533 399 311 308 85
63
BspEI T'CCGG_A
Cuts at: 5349 5349
Size: 5686
BspHI T'CATG_A
Cuts at: 2749 3575 5305 2749
Size: 826 1730 3130
Fragments arranged by size:
3130 1730 826
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2725 3041 844
Size: 1881 316 3489
Fragments arranged by size:
3489 1881 316
BsrBI CCG'CTC
Cuts at: 1394 1823 2119 2180 3573 1394
Size: 429 296 61 1393 3507
Fragments arranged by size:
3507 1393 429 296 61
BsrDI GCAATG_nn'
Cuts at: 65 1248 4181 4355 65
Size: 1183 2933 174 1396
Fragments arranged by size:
2933 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2068 2552 4330 4517 1700
Size: 110 258 484 1778 187 2869
Fragments arranged by size:
2869 1778 484 258 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5686
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5686
BssSI C'ACGA_G
Cuts at: 3746 4758 3746
Size: 1012 4674
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2080 2233 2619 2731 794
Size: 1286 153 386 112 3749
Fragments arranged by size:
3749 1286 386 153 112
BstBI TT'CG_AA
Cuts at: 4490 4490
Size: 5686
BstEII G'GTnAC_C
Cuts at: 1184 2182 1184
Size: 998 4688
BstYI r'GATC_y
Cuts at: 1972 2802 3016 3383 3768 3785 5237 5323 5335
Size: 830 214 367 385 17 1452 86 12
Cuts at: 5335 5681 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 830 385 367 346 214 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2200 2685 2934 513
Size: 552 1135 485 249 3265
Fragments arranged by size:
3265 1135 552 485 249
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2599 487
Size: 683 56 1373 3574
Fragments arranged by size:
3574 1373 683 56
BtsI GCAGTG_nn'
Cuts at: 3202 3996 4016 3202
Size: 794 20 4872
Fragments arranged by size:
4872 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5686
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5651
DraI TTT'AAA
Cuts at: 2990 2990
Size: 5686
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4693 809
Size: 507 3377 1802
Fragments arranged by size:
3377 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2197 2206 2367
Size: 54 1425 50 275 18 367 9 161
Cuts at: 2367 2550 2789 2808 2868 2931 3133 4022 5651
Size: 183 239 19 60 63 202 889 1629
Cuts at: 5651 8
Size: 43
Fragments arranged by size:
1629 1425 889 367 275 239 202 183
161 63 60 54 50 43 19 18
9
EagI C'GGCC_G
Cuts at: 1537 1812 3133 1537
Size: 275 1321 4090
Fragments arranged by size:
4090 1321 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2129 3616 1353
Size: 776 1487 3423
Fragments arranged by size:
3423 1487 776
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2866 4273 4647 4793 387
Size: 1064 138 1277 1407 374 146 1280
Fragments arranged by size:
1407 1280 1277 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2016 2179 2294 2453 2486 2728 3749
Size: 393 384 163 115 159 33 242 1021
Cuts at: 3749 4346 5133 5401 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1021 787 597 393 384 268 242
163 159 115 33
EcoNI CCTnn'n_nnAGG
Cuts at: 2258 2297 2258
Size: 39 5647
EcoO109I rG'GnC_Cy
Cuts at: 2269 2290 2728 2269
Size: 21 438 5227
Fragments arranged by size:
5227 438 21
EcoRI G'AATT_C
Cuts at: 2995 3373 2995
Size: 378 5308
FspI TGC'GCA
Cuts at: 4192 4192
Size: 5686
HaeII r_GCGC'y
Cuts at: 1678 2116 2248 2313 2699 2892 4833 1678
Size: 438 132 65 386 193 1941 2531
Fragments arranged by size:
2531 1941 438 386 193 132 65
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2516 2548 2552 2559 2584
Size: 32 135 32 944 32 4 7 25
Cuts at: 2584 2591 2639 2671 4327 4359 4401 4433 1373
Size: 7 48 32 1656 32 42 32 2626
Fragments arranged by size:
2626 1656 944 135 48 42 32 32
32 32 32 32 25 7 7 4
HincII GTy'rAC
Cuts at: 669 1657 1938 3030 3276 669
Size: 988 281 1092 246 3079
Fragments arranged by size:
3079 1092 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5686
HpaI GTT'AAC
Cuts at: 3276 3276
Size: 5686
KasI G'GCGC_C
Cuts at: 2112 2244 2112
Size: 132 5554
KpnI G_GTAC'C
Cuts at: 3011 3011
Size: 5686
MfeI C'AATT_G
Cuts at: 3285 3285
Size: 5686
MluI A'CGCG_T
Cuts at: 5562 5562
Size: 5686
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4800 4984 1305
Size: 3495 184 2007
Fragments arranged by size:
3495 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2199 2810 10
Size: 54 1425 343 367 611 2886
Fragments arranged by size:
2886 1425 611 367 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2498 2837 3644 4003 4162 518
Size: 648 1332 339 807 359 159 2042
Fragments arranged by size:
2042 1332 807 648 359 339 159
MspA1I CmG'CkG
Cuts at: 1606 2687 2894 2936 2953 3102 3779 4927 5172
Size: 1081 207 42 17 149 677 1148 245
Cuts at: 5172 1606
Size: 2120
Fragments arranged by size:
2120 1148 1081 677 245 207 149 42
17
NaeI GCC'GGC
Cuts at: 2554 2554
Size: 5686
NarI GG'CG_CC
Cuts at: 2113 2245 2113
Size: 132 5554
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5134
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5686
NgoMIV G'CCGG_C
Cuts at: 2552 2552
Size: 5686
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5686
NotI GC'GGCC_GC
Cuts at: 3133 3133
Size: 5686
NspI r_CATG'y
Cuts at: 1132 1229 2078 2497 3044 4589 1132
Size: 97 849 419 547 1545 2229
Fragments arranged by size:
2229 1545 849 547 419 97
PasI CC'CwG_GG
Cuts at: 1348 2239 1348
Size: 891 4795
PciI A'CATG_T
Cuts at: 4585 4585
Size: 5686
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2905 1108
Size: 1797 3889
PmeI GTTT'AAAC
Cuts at: 2990 2990
Size: 5686
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3755 3787 5268 5300 3755
Size: 32 1481 32 4141
Fragments arranged by size:
4141 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2269 2290 2269
Size: 21 5665
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5686
PsiI TTA'TAA
Cuts at: 3256 3256
Size: 5686
PstI C_TGCA'G
Cuts at: 830 2169 2229 2531 2736 3038 830
Size: 1339 60 302 205 302 3478
Fragments arranged by size:
3478 1339 302 302 205 60
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5686
SacII CC_GC'GG
Cuts at: 2688 2937 2688
Size: 249 5437
SalI G'TCGA_C
Cuts at: 1936 3028 1936
Size: 1092 4594
SbfI CC_TGCA'GG
Cuts at: 2736 3038 2736
Size: 302 5384
ScaI AGT'ACT
Cuts at: 3934 5646 3934
Size: 1712 3974
SfcI C'TryA_G
Cuts at: 826 1045 2165 2225 2527 2732 3034 4169 4850
Size: 219 1120 60 302 205 302 1135 681
Cuts at: 4850 5041 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1120 681 302 302 219 205
191 60
SfiI GGCCn_nnn'nGGCC
Cuts at: 2205 2876 2205
Size: 671 5015
SfoI GGC'GCC
Cuts at: 2114 2246 2114
Size: 132 5554
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5686
SgrAI Cr'CCGG_yG
Cuts at: 1700 2068 1700
Size: 368 5318
SmaI CCC'GGG
Cuts at: 1872 2767 3013 1872
Size: 895 246 4545
Fragments arranged by size:
4545 895 246
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3790 4691 4953 820
Size: 197 926 1847 901 262 1553
Fragments arranged by size:
1847 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3477 493
Size: 2984 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5686
SphI G_CATG'C
Cuts at: 1132 2078 2497 3044 1132
Size: 946 419 547 3774
Fragments arranged by size:
3774 946 547 419
SspI AAT'ATT
Cuts at: 5 52 3610 5
Size: 47 3558 2081
Fragments arranged by size:
3558 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5686
StyI C'CwwG_G
Cuts at: 513 1065 2943 3125 513
Size: 552 1878 182 3074
Fragments arranged by size:
3074 1878 552 182
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2075 3713 3730 3883 4068 4513 325
Size: 1255 495 1638 17 153 185 445 1498
Fragments arranged by size:
1638 1498 1255 495 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3932 5644 96
Size: 274 80 33 51 1443 1955 1712 138
Fragments arranged by size:
1955 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2312 2344 5646 5678 2312
Size: 32 3302 32 2320
Fragments arranged by size:
3302 2320 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5686
XbaI T'CTAG_A
Cuts at: 3022 3022
Size: 5686
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5686
XmaI C'CCGG_G
Cuts at: 1870 2765 3011 1870
Size: 895 246 4545
Fragments arranged by size:
4545 895 246
XmnI GAAnn'nnTTC
Cuts at: 3815 3815
Size: 5686
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2451 2587 3494 276
Size: 53 83 186 936 917 136 907 2468
Fragments arranged by size:
2468 936 917 907 186 136 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI
BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI
FspAI NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI PvuII RsrII SacI SanDI SapI SexAI SrfI
SwaI XcmI