Restriction map for
pFN21AA1625
(Circular) (Six-base) MAPSORT of: pFN21AA1625.seq Check: 4964 from: 1 to: 6976
pFN21AA1625 6976 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4786 278
Size: 53 83 186 936 3250 2468
Fragments arranged by size:
3250 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4319 1937
Size: 2382 4594
Acc65I G'GTAC_C
Cuts at: 4297 4297
Size: 6976
AclI AA'CG_TT
Cuts at: 1563 5103 5476 1563
Size: 3540 373 3063
Fragments arranged by size:
3540 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2080 2609 3161 3884 3971 5039 6423 6691
Size: 448 529 552 723 87 1068 1384 268
Cuts at: 6691 1632
Size: 1917
Fragments arranged by size:
1917 1384 1068 723 552 529 448 268
87
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6779
AflIII A'CryG_T
Cuts at: 1203 3417 5875 6852 1203
Size: 2214 2458 977 1327
Fragments arranged by size:
2458 2214 1327 977
AgeI A'CCGG_T
Cuts at: 2561 5807 2561
Size: 3246 3730
AhdI GACnn_n'nnGTC
Cuts at: 3460 3934 5705 3460
Size: 474 1771 4731
Fragments arranged by size:
4731 1771 474
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6976
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3749 3995 1184
Size: 2565 246 4165
Fragments arranged by size:
4165 2565 246
AlwNI CAG_nnn'CTG
Cuts at: 2381 2960 3070 3148 3559 4719 6291 2381
Size: 579 110 78 411 1160 1572 3066
Fragments arranged by size:
3066 1572 1160 579 411 110 78
ApaI G_GGCC'C
Cuts at: 4116 4116
Size: 6976
ApaLI G'TGCA_C
Cuts at: 5033 6189 5033
Size: 1156 5820
ApoI r'AATT_y
Cuts at: 1493 2259 2855 3296 4285 4511 4663 6772 1493
Size: 766 596 441 989 226 152 2109 1697
Fragments arranged by size:
2109 1697 989 766 596 441 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5530 160
Size: 872 4498 1606
Fragments arranged by size:
4498 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6976
AvaI C'yCGr_G
Cuts at: 1870 1943 2015 2815 4301 1870
Size: 73 72 800 1486 4545
Fragments arranged by size:
4545 1486 800 73 72
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2332 2365 2332
Size: 33 6943
BamHI G'GATC_C
Cuts at: 3627 3696 4306 4673 3627
Size: 69 610 367 5930
Fragments arranged by size:
5930 610 367 69
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4297 5752 618
Size: 325 217 627 2510 1455 1842
Fragments arranged by size:
2510 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2886 3060 3092 3390 3846 4116 4295 1391
Size: 1495 174 32 298 456 270 179 4072
Fragments arranged by size:
4072 1495 456 298 270 179 174 32
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2502 928
Size: 971 603 5402
Fragments arranged by size:
5402 971 603
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5167 5201 1903
Size: 34 3230 34 3678
Fragments arranged by size:
3678 3230 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2333 2796 4868 6078 2333
Size: 463 2072 1210 3231
Fragments arranged by size:
3231 2072 1210 463
BclI T'GATC_A
Cuts at: 1548 3894 1548
Size: 2346 4630
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2512 5587 136
Size: 107 122 71 631 605 840 3075 1525
Fragments arranged by size:
3075 1525 840 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3133 3583 6971 3133
Size: 450 3388 3138
Fragments arranged by size:
3388 3138 450
BlpI GC'TnA_GC
Cuts at: 2519 4393 2519
Size: 1874 5102
Bme1580I G_kGCm'C
Cuts at: 1790 3543 4116 5037 6193 1790
Size: 1753 573 921 1156 2573
Fragments arranged by size:
2573 1753 1156 921 573
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6976
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2995 3075 3805
Size: 416 53 83 399 24 1578 80 730
Cuts at: 3805 4059 4707 5665 442
Size: 254 648 958 1753
Fragments arranged by size:
1753 1578 958 730 648 416 399 254
83 80 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6976
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4085 4085
Size: 6976
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2020 2595 2796 3116 3805 3908 5636 1239
Size: 781 575 201 320 689 103 1728 2579
Fragments arranged by size:
2579 1728 781 689 575 320 201 103
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3035 5101 5966 6264 3035
Size: 2066 865 298 3747
Fragments arranged by size:
3747 2066 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2883 4247 5639 882
Size: 617 1384 1364 1392 2219
Fragments arranged by size:
2219 1392 1384 1364 617
BsaAI yAC'GTr
Cuts at: 493 1204 2787 4767 493
Size: 711 1583 1980 2702
Fragments arranged by size:
2702 1980 1583 711
BsaBI GATnn'nnATC
Cuts at: 6970 6970
Size: 6976
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2672 4783 5165 5800
Size: 53 83 186 936 1139 2111 382 635
Cuts at: 5800 275
Size: 1451
Fragments arranged by size:
2111 1451 1139 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2336 2561 3433 3768 4185 5409 5807 6081 6228
Size: 225 872 335 417 1224 398 274 147
Cuts at: 6228 6639 2336
Size: 411 2673
Fragments arranged by size:
2673 1224 872 417 411 398 335 274
225 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6751
Fragments arranged by size:
6751 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2592 3119 3248 3761 4265 1186
Size: 1406 527 129 513 504 3897
Fragments arranged by size:
3897 1406 527 513 504 129
BseYI C'CCAG_C
Cuts at: 2937 3059 3559 6179 6675 2937
Size: 122 500 2620 496 3238
Fragments arranged by size:
3238 2620 500 496 122
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6403
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3266 4426 5187 6215 1540
Size: 275 186 1265 1160 761 1028 2301
Fragments arranged by size:
2301 1265 1160 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3846 5037 5122 6193 3846
Size: 1191 85 1071 4629
Fragments arranged by size:
4629 1191 1071 85
BsmI GAATG_Cn'
Cuts at: 4487 4580 4487
Size: 93 6883
BsmBI CGTCTCn'nnnn_
Cuts at: 3998 3998
Size: 6976
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2886 3060 3092 3390 3543 3846 4116
Size: 399 1096 174 32 298 153 303 270
Cuts at: 4116 4295 5037 5122 6193 1391
Size: 179 742 85 1071 2174
Fragments arranged by size:
2174 1096 1071 742 399 303 298 270
179 174 153 85 32
BspEI T'CCGG_A
Cuts at: 3433 6639 3433
Size: 3206 3770
BspHI T'CATG_A
Cuts at: 3030 4865 6595 3030
Size: 1835 1730 3411
Fragments arranged by size:
3411 1835 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2580 4184 4265 4331 844
Size: 1736 1604 81 66 3489
Fragments arranged by size:
3489 1736 1604 81 66
BsrBI CCG'CTC
Cuts at: 1394 1823 2518 4069 4863 1394
Size: 429 695 1551 794 3507
Fragments arranged by size:
3507 1551 794 695 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3196 5471 5645 65
Size: 1183 1948 2275 174 1396
Fragments arranged by size:
2275 1948 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2438 2561 5620 5807 1700
Size: 110 628 123 3059 187 2869
Fragments arranged by size:
3059 2869 628 187 123 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6976
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6976
BssSI C'ACGA_G
Cuts at: 2421 5036 6048 2421
Size: 2615 1012 3349
Fragments arranged by size:
3349 2615 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6976
BstBI TT'CG_AA
Cuts at: 2641 5780 2641
Size: 3139 3837
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6976
BstYI r'GATC_y
Cuts at: 1972 2046 2353 3133 3583 3627 3696 4306 4673
Size: 74 307 780 450 44 69 610 367
Cuts at: 4673 5058 5075 6527 6613 6625 6971 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 780 610 450 385 367 346
307 86 74 69 44 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3301 3508 4245 513
Size: 552 2236 207 737 3244
Fragments arranged by size:
3244 2236 737 552 207
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6237
Fragments arranged by size:
6237 683 56
BtsI GCAGTG_nn'
Cuts at: 2150 2924 3074 4492 5286 5306 2150
Size: 774 150 1418 794 20 3820
Fragments arranged by size:
3820 1418 794 774 150 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6976
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6941
DraI TTT'AAA
Cuts at: 4280 4280
Size: 6976
DraIII CAC_nnn'GTG
Cuts at: 3478 3961 3478
Size: 483 6493
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5983 809
Size: 507 4667 1802
Fragments arranged by size:
4667 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2395 2692 3333
Size: 54 1425 50 275 18 565 297 641
Cuts at: 3333 3963 4423 5312 6941 8
Size: 630 460 889 1629 43
Fragments arranged by size:
1629 1425 889 641 630 565 460 297
275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4423 1537
Size: 275 2611 4090
Fragments arranged by size:
4090 2611 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3395 4906 1353
Size: 2042 1511 3423
Fragments arranged by size:
3423 2042 1511
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3499 3709 5563 5937 6083 387
Size: 1064 138 1910 210 1854 374 146 1280
Fragments arranged by size:
1910 1854 1280 1064 374 210 146 138
EcoICRI GAG'CTC
Cuts at: 3844 3844
Size: 6976
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2020 2080 2595 2609 2796 3116 3161
Size: 393 388 60 515 14 187 320 45
Cuts at: 3161 3805 3884 3908 3971 5039 5636 6423 6691
Size: 644 79 24 63 1068 597 787 268
Cuts at: 6691 1239
Size: 1524
Fragments arranged by size:
1524 1068 787 644 597 515 393 388
320 268 187 79 63 60 45 24
14
EcoNI CCTnn'n_nnAGG
Cuts at: 4163 4163
Size: 6976
EcoO109I rG'GnC_Cy
Cuts at: 4112 4113 4112
Size: 1 6975
EcoRI G'AATT_C
Cuts at: 2855 4285 4663 2855
Size: 1430 378 5168
Fragments arranged by size:
5168 1430 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2107 2107
Size: 6976
FspI TGC'GCA
Cuts at: 5482 5482
Size: 6976
HaeII r_GCGC'y
Cuts at: 1678 6123 1678
Size: 4445 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2794 2826 3123 3155 3210
Size: 32 135 32 1222 32 297 32 55
Cuts at: 3210 3242 3446 3478 3504 3536 3575 3607 3920
Size: 32 204 32 26 32 39 32 313
Cuts at: 3920 3952 5617 5649 5691 5723 1373
Size: 32 1665 32 42 32 2626
Fragments arranged by size:
2626 1665 1222 313 297 204 135 55
42 39 32 32 32 32 32 32
32 32 32 32 32 26
HincII GTy'rAC
Cuts at: 669 1657 1938 4320 4566 669
Size: 988 281 2382 246 3079
Fragments arranged by size:
3079 2382 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6976
HpaI GTT'AAC
Cuts at: 4566 4566
Size: 6976
KpnI G_GTAC'C
Cuts at: 4301 4301
Size: 6976
MfeI C'AATT_G
Cuts at: 4575 4575
Size: 6976
MluI A'CGCG_T
Cuts at: 6852 6852
Size: 6976
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6090 6274 1305
Size: 4785 184 2007
Fragments arranged by size:
4785 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2397 3335 10
Size: 54 1425 343 565 938 3651
Fragments arranged by size:
3651 1425 938 565 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2240 3209 4934 5293 5452 518
Size: 648 1074 969 1725 359 159 2042
Fragments arranged by size:
2042 1725 1074 969 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2523 2635 3070 3556 4392 5069 6217 6462
Size: 917 112 435 486 836 677 1148 245
Cuts at: 6462 1606
Size: 2120
Fragments arranged by size:
2120 1148 917 836 677 486 435 245
112
NcoI C'CATG_G
Cuts at: 513 1065 3508 513
Size: 552 2443 3981
Fragments arranged by size:
3981 2443 552
NdeI CA'TA_TG
Cuts at: 387 4204 387
Size: 3817 3159
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6976
NotI GC'GGCC_GC
Cuts at: 4423 4423
Size: 6976
NspI r_CATG'y
Cuts at: 1132 1229 3421 4334 5879 1132
Size: 97 2192 913 1545 2229
Fragments arranged by size:
2229 2192 1545 913 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6976
PciI A'CATG_T
Cuts at: 3417 5875 3417
Size: 2458 4518
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2350 2404 3342 3478 1108
Size: 1242 54 938 136 4606
Fragments arranged by size:
4606 1242 938 136 54
PmeI GTTT'AAAC
Cuts at: 4280 4280
Size: 6976
PmlI CAC'GTG
Cuts at: 2787 2787
Size: 6976
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5045 5077 6558 6590 5045
Size: 32 1481 32 5431
Fragments arranged by size:
5431 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6976
PsiI TTA'TAA
Cuts at: 4546 4546
Size: 6976
PspOMI G'GGCC_C
Cuts at: 4112 4112
Size: 6976
PstI C_TGCA'G
Cuts at: 830 2462 3705 3828 4041 4328 830
Size: 1632 1243 123 213 287 3478
Fragments arranged by size:
3478 1632 1243 287 213 123
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6976
PvuII CAG'CTG
Cuts at: 2523 3556 2523
Size: 1033 5943
SacI G_AGCT'C
Cuts at: 3846 3846
Size: 6976
SalI G'TCGA_C
Cuts at: 1936 4318 1936
Size: 2382 4594
SapI GCTCTTCn'nnn_
Cuts at: 3395 3395
Size: 6976
SbfI CC_TGCA'GG
Cuts at: 4328 4328
Size: 6976
ScaI AGT'ACT
Cuts at: 5224 6936 5224
Size: 1712 5264
SfcI C'TryA_G
Cuts at: 826 1045 2458 2666 3701 3824 4037 4098 4324
Size: 219 1413 208 1035 123 213 61 226
Cuts at: 4324 5459 6140 6331 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1413 1135 1035 681 226 219 213
208 191 123 61
SfiI GGCCn_nnn'nGGCC
Cuts at: 2512 2512
Size: 6976
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6976
SgrAI Cr'CCGG_yG
Cuts at: 1700 2561 1700
Size: 861 6115
SmaI CCC'GGG
Cuts at: 1872 2017 4303 1872
Size: 145 2286 4545
Fragments arranged by size:
4545 2286 145
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3050 5080 5981 6243 820
Size: 197 926 1107 2030 901 262 1553
Fragments arranged by size:
2030 1553 1107 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4767 493
Size: 4274 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6976
SphI G_CATG'C
Cuts at: 1132 4334 1132
Size: 3202 3774
SspI AAT'ATT
Cuts at: 5 52 3412 4900 5
Size: 47 3360 1488 2081
Fragments arranged by size:
3360 2081 1488 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6976
StyI C'CwwG_G
Cuts at: 513 1065 3014 3508 4072 4415 513
Size: 552 1949 494 564 343 3074
Fragments arranged by size:
3074 1949 564 552 494 343
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3280 5003 5020 5173 5358 5803 325
Size: 1255 1700 1723 17 153 185 445 1498
Fragments arranged by size:
1723 1700 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2951 3652 5222
Size: 274 80 33 51 1443 974 701 1570
Cuts at: 5222 6934 96
Size: 1712 138
Fragments arranged by size:
1712 1570 1443 974 701 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2171 2203 6936 6968 2171
Size: 32 4733 32 2179
Fragments arranged by size:
4733 2179 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4051 1316
Size: 2735 4241
XbaI T'CTAG_A
Cuts at: 4312 4312
Size: 6976
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6976
XmaI C'CCGG_G
Cuts at: 1870 2015 4301 1870
Size: 145 2286 4545
Fragments arranged by size:
4545 2286 145
XmnI GAAnn'nnTTC
Cuts at: 2090 2859 3833 5105 2090
Size: 769 974 1272 3961
Fragments arranged by size:
3961 1272 974 769
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4784 276
Size: 53 83 186 936 3250 2468
Fragments arranged by size:
3250 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI
Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfoI
SrfI SwaI XcmI