Restriction map for
pFN21AA1624
(Circular) (Six-base) MAPSORT of: pFN21AA1624.seq Check: 7226 from: 1 to: 6505
pFN21AA1624 6505 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4315 278
Size: 53 83 186 936 2779 2468
Fragments arranged by size:
2779 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3225 3848 1937
Size: 1288 623 4594
Fragments arranged by size:
4594 1288 623
Acc65I G'GTAC_C
Cuts at: 2420 3826 2420
Size: 1406 5099
AclI AA'CG_TT
Cuts at: 1563 4632 5005 1563
Size: 3069 373 3063
Fragments arranged by size:
3069 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2262 2428 2484 3314 3636 4568 5952 6220
Size: 630 166 56 830 322 932 1384 268
Cuts at: 6220 1632
Size: 1917
Fragments arranged by size:
1917 1384 932 830 630 322 268 166
56
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6308
AflIII A'CryG_T
Cuts at: 1203 5404 6381 1203
Size: 4201 977 1327
Fragments arranged by size:
4201 1327 977
AgeI A'CCGG_T
Cuts at: 5336 5336
Size: 6505
AhdI GACnn_n'nnGTC
Cuts at: 5234 5234
Size: 6505
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6505
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6505
AlwNI CAG_nnn'CTG
Cuts at: 2101 2417 3139 3592 4248 5820 2101
Size: 316 722 453 656 1572 2786
Fragments arranged by size:
2786 1572 722 656 453 316
ApaLI G'TGCA_C
Cuts at: 4562 5718 4562
Size: 1156 5349
ApoI r'AATT_y
Cuts at: 1493 2781 3638 3814 4040 4192 6301 1493
Size: 1288 857 176 226 152 2109 1697
Fragments arranged by size:
2109 1697 1288 857 226 176 152
AseI AT'TA_AT
Cuts at: 160 1032 5059 160
Size: 872 4027 1606
Fragments arranged by size:
4027 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6505
AvaI C'yCGr_G
Cuts at: 1870 1943 3384 3830 1870
Size: 73 1441 446 4545
Fragments arranged by size:
4545 1441 446 73
AvrII C'CTAG_G
Cuts at: 2048 2048
Size: 6505
BamHI G'GATC_C
Cuts at: 3835 4202 3835
Size: 367 6138
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2017 2420 3242 3797 3826
Size: 325 217 627 230 403 822 555 29
Cuts at: 3826 5281 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 822 627 555 403 325 230
217 29
BanII G_rGCy'C
Cuts at: 1391 3780 3824 1391
Size: 2389 44 4072
Fragments arranged by size:
4072 2389 44
BbeI G_GCGC'C
Cuts at: 2021 3801 2021
Size: 1780 4725
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2250 2528 3607 3696 928
Size: 971 351 278 1079 89 3737
Fragments arranged by size:
3737 1079 971 351 278 89
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4696 4730 1903
Size: 34 2759 34 3678
Fragments arranged by size:
3678 2759 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4397 5607 4397
Size: 1210 5295
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6505
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2015 5116 136
Size: 107 122 71 631 605 343 3101 1525
Fragments arranged by size:
3101 1525 631 605 343 122 107 71
BglII A'GATC_T
Cuts at: 3108 6500 3108
Size: 3392 3113
BlpI GC'TnA_GC
Cuts at: 3922 3922
Size: 6505
Bme1580I G_kGCm'C
Cuts at: 1790 3185 4566 5722 1790
Size: 1395 1381 1156 2573
Fragments arranged by size:
2573 1395 1381 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6505
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2419 3251 4236
Size: 416 53 83 399 24 1002 832 985
Cuts at: 4236 5194 442
Size: 958 1753
Fragments arranged by size:
1753 1002 985 958 832 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6505
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2365 3116 5165 1239
Size: 1126 751 2049 2579
Fragments arranged by size:
2579 2049 1126 751
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2126 4630 5495 5793 2126
Size: 2504 865 298 2838
Fragments arranged by size:
2838 2504 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5168 882
Size: 617 3669 2219
Fragments arranged by size:
3669 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4296 493
Size: 711 3092 2702
Fragments arranged by size:
3092 2702 711
BsaBI GATnn'nnATC
Cuts at: 2849 6499 2849
Size: 3650 2855
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2018 3798 4312 4694
Size: 53 83 186 936 485 1780 514 382
Cuts at: 4694 5329 275
Size: 635 1451
Fragments arranged by size:
1780 1451 936 635 514 485 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4938 5336 5610 5757 6168 4938
Size: 398 274 147 411 5275
Fragments arranged by size:
5275 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2945 2948 2975 2978 1178
Size: 30 165 30 1542 3 27 3 4705
Fragments arranged by size:
4705 1542 165 30 30 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2953 3191 3536 1186
Size: 1767 238 345 4155
Fragments arranged by size:
4155 1767 345 238
BseYI C'CCAG_C
Cuts at: 2083 2988 3066 3443 5708 6204 2083
Size: 905 78 377 2265 496 2384
Fragments arranged by size:
2384 2265 905 496 377 78
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5932
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3955 4716 5744 1540
Size: 275 186 1954 761 1028 2301
Fragments arranged by size:
2301 1954 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4566 4651 5722 4566
Size: 85 1071 5349
Fragments arranged by size:
5349 1071 85
BsmI GAATG_Cn'
Cuts at: 4016 4109 4016
Size: 93 6412
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3185 3780 3824 4566 4651 5722 1391
Size: 399 1395 595 44 742 85 1071 2174
Fragments arranged by size:
2174 1395 1071 742 595 399 85 44
BspEI T'CCGG_A
Cuts at: 6168 6168
Size: 6505
BspHI T'CATG_A
Cuts at: 3114 4394 6124 3114
Size: 1280 1730 3495
Fragments arranged by size:
3495 1730 1280
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3860 844
Size: 3016 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2012 4392 1394
Size: 429 189 2380 3507
Fragments arranged by size:
3507 2380 429 189
BsrDI GCAATG_nn'
Cuts at: 65 1248 3045 3534 5000 5174 65
Size: 1183 1797 489 1466 174 1396
Fragments arranged by size:
1797 1466 1396 1183 489 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5149 5336 1700
Size: 110 3339 187 2869
Fragments arranged by size:
3339 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6505
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6505
BssSI C'ACGA_G
Cuts at: 4565 5577 4565
Size: 1012 5493
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6505
BstBI TT'CG_AA
Cuts at: 2857 5309 2857
Size: 2452 4053
BstEII G'GTnAC_C
Cuts at: 1184 3757 1184
Size: 2573 3932
BstXI CCAn_nnnn'nTGG
Cuts at: 3264 3443 3570 3264
Size: 179 127 6199
Fragments arranged by size:
6199 179 127
BstYI r'GATC_y
Cuts at: 1972 3108 3835 4202 4587 4604 6056 6142 6154
Size: 1136 727 367 385 17 1452 86 12
Cuts at: 6154 6500 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1136 727 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2041 2058 2571 3771 3801 513
Size: 552 939 37 17 513 1200 30 3217
Fragments arranged by size:
3217 1200 939 552 513 37 30 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5766
Fragments arranged by size:
5766 683 56
BtsI GCAGTG_nn'
Cuts at: 2619 4021 4815 4835 2619
Size: 1402 794 20 4289
Fragments arranged by size:
4289 1402 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6505
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6470
DraI TTT'AAA
Cuts at: 2337 2881 3809 2337
Size: 544 928 5033
Fragments arranged by size:
5033 928 544
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5512 809
Size: 507 4196 1802
Fragments arranged by size:
4196 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2055 2103
Size: 54 1425 50 275 18 177 48 48
Cuts at: 2103 3603 3952 4841 6470 8
Size: 1500 349 889 1629 43
Fragments arranged by size:
1629 1500 1425 889 349 275 177 54
50 48 48 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3952 1537
Size: 275 2140 4090
Fragments arranged by size:
4090 2140 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3041 3257 3333 4435 1353
Size: 1688 216 76 1102 3423
Fragments arranged by size:
3423 1688 1102 216 76
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5092 5466 5612 387
Size: 1064 138 3503 374 146 1280
Fragments arranged by size:
3503 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2262 2365 2428 2484 3116 3314 3636
Size: 393 630 103 63 56 632 198 322
Cuts at: 3636 4568 5165 5952 6220 1239
Size: 932 597 787 268 1524
Fragments arranged by size:
1524 932 787 632 630 597 393 322
268 198 103 63 56
EcoNI CCTnn'n_nnAGG
Cuts at: 2982 2982
Size: 6505
EcoO109I rG'GnC_Cy
Cuts at: 3136 3323 3136
Size: 187 6318
EcoRI G'AATT_C
Cuts at: 3814 4192 3814
Size: 378 6127
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2458 2678 2458
Size: 220 6285
FspI TGC'GCA
Cuts at: 5011 5011
Size: 6505
HaeII r_GCGC'y
Cuts at: 1678 2021 3032 3801 5652 1678
Size: 343 1011 769 1851 2531
Fragments arranged by size:
2531 1851 1011 769 343
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3328 3360 5146 5178 5220
Size: 32 135 32 1756 32 1786 32 42
Cuts at: 5220 5252 1373
Size: 32 2626
Fragments arranged by size:
2626 1786 1756 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2491 3226 3849 4095 669
Size: 988 281 553 735 623 246 3079
Fragments arranged by size:
3079 988 735 623 553 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6505
HpaI GTT'AAC
Cuts at: 2491 4095 2491
Size: 1604 4901
KasI G'GCGC_C
Cuts at: 2017 3797 2017
Size: 1780 4725
KpnI G_GTAC'C
Cuts at: 2424 3830 2424
Size: 1406 5099
MfeI C'AATT_G
Cuts at: 2943 4104 2943
Size: 1161 5344
MluI A'CGCG_T
Cuts at: 6381 6381
Size: 6505
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5619 5803 1305
Size: 4314 184 2007
Fragments arranged by size:
4314 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4683
Fragments arranged by size:
4683 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2207 2601 2807 3568 4463 4822 4981
Size: 648 1041 394 206 761 895 359 159
Cuts at: 4981 518
Size: 2042
Fragments arranged by size:
2042 1041 895 761 648 394 359 206
159
MspA1I CmG'CkG
Cuts at: 1606 2043 2060 3455 3921 4598 5746 5991 1606
Size: 437 17 1395 466 677 1148 245 2120
Fragments arranged by size:
2120 1395 1148 677 466 437 245 17
NarI GG'CG_CC
Cuts at: 2018 3798 2018
Size: 1780 4725
NcoI C'CATG_G
Cuts at: 513 1065 2004 2571 513
Size: 552 939 567 4447
Fragments arranged by size:
4447 939 567 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6505
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6505
NotI GC'GGCC_GC
Cuts at: 3952 3952
Size: 6505
NspI r_CATG'y
Cuts at: 1132 1229 3863 5408 1132
Size: 97 2634 1545 2229
Fragments arranged by size:
2634 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2968 3781 1348
Size: 1620 813 4072
Fragments arranged by size:
4072 1620 813
PciI A'CATG_T
Cuts at: 5404 5404
Size: 6505
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2134 2417 3139 3592 1108
Size: 1026 283 722 453 4021
Fragments arranged by size:
4021 1026 722 453 283
PfoI T'CCnGG_A
Cuts at: 3591 3591
Size: 6505
PmeI GTTT'AAAC
Cuts at: 3809 3809
Size: 6505
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4574 4606 6087 6119 4574
Size: 32 1481 32 4960
Fragments arranged by size:
4960 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3136 3323 3136
Size: 187 6318
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6505
PsiI TTA'TAA
Cuts at: 4075 4075
Size: 6505
PstI C_TGCA'G
Cuts at: 830 2097 3857 830
Size: 1267 1760 3478
Fragments arranged by size:
3478 1760 1267
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6505
PvuII CAG'CTG
Cuts at: 3455 3455
Size: 6505
SacII CC_GC'GG
Cuts at: 2044 2061 2044
Size: 17 6488
SalI G'TCGA_C
Cuts at: 1936 3224 3847 1936
Size: 1288 623 4594
Fragments arranged by size:
4594 1288 623
SbfI CC_TGCA'GG
Cuts at: 3857 3857
Size: 6505
ScaI AGT'ACT
Cuts at: 4753 6465 4753
Size: 1712 4793
SfcI C'TryA_G
Cuts at: 826 1045 2093 3853 4988 5669 5860 826
Size: 219 1048 1760 1135 681 191 1471
Fragments arranged by size:
1760 1471 1135 1048 681 219 191
SfoI GGC'GCC
Cuts at: 2019 3799 2019
Size: 1780 4725
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6505
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6505
SmaI CCC'GGG
Cuts at: 1872 3386 3832 1872
Size: 1514 446 4545
Fragments arranged by size:
4545 1514 446
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2141 4609 5510 5772 820
Size: 197 926 198 2468 901 262 1553
Fragments arranged by size:
2468 1553 926 901 262 198 197
SnaBI TAC'GTA
Cuts at: 493 4296 493
Size: 3803 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6505
SphI G_CATG'C
Cuts at: 1132 3863 1132
Size: 2731 3774
SrfI GCCC'GGGC
Cuts at: 3386 3386
Size: 6505
SspI AAT'ATT
Cuts at: 5 52 4429 5
Size: 47 4377 2081
Fragments arranged by size:
4377 2081 47
StuI AGG'CCT
Cuts at: 1517 2661 1517
Size: 1144 5361
StyI C'CwwG_G
Cuts at: 513 1065 2004 2048 2464 2571 2689 2928 3944
Size: 552 939 44 416 107 118 239 1016
Cuts at: 3944 513
Size: 3074
Fragments arranged by size:
3074 1016 939 552 416 239 118 107
44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4532 4549 4702 4887 5332 325
Size: 1255 2952 17 153 185 445 1498
Fragments arranged by size:
2952 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2552 3000 4751
Size: 274 80 33 51 1443 575 448 1751
Cuts at: 4751 6463 96
Size: 1712 138
Fragments arranged by size:
1751 1712 1443 575 448 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2945 2977 3120 3152 6465 6497 2945
Size: 32 143 32 3313 32 2953
Fragments arranged by size:
3313 2953 143 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6505
XbaI T'CTAG_A
Cuts at: 3841 3841
Size: 6505
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 3769 2011
Size: 1758 4747
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6505
XmaI C'CCGG_G
Cuts at: 1870 3384 3830 1870
Size: 1514 446 4545
Fragments arranged by size:
4545 1514 446
XmnI GAAnn'nnTTC
Cuts at: 3482 4634 3482
Size: 1152 5353
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4313 276
Size: 53 83 186 936 2779 2468
Fragments arranged by size:
2779 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SacI SanDI SapI
SexAI SfiI SwaI