Restriction map for
pFN21AA1621
(Circular) (Six-base) MAPSORT of: pFN21AA1621.seq Check: 6918 from: 1 to: 7033
pFN21AA1621 7033 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3001 3504 3001
Size: 503 6530
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4843 278
Size: 53 83 186 936 3307 2468
Fragments arranged by size:
3307 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4068 4376 1937
Size: 2131 308 4594
Fragments arranged by size:
4594 2131 308
Acc65I G'GTAC_C
Cuts at: 4354 4354
Size: 7033
AclI AA'CG_TT
Cuts at: 1563 5160 5533 1563
Size: 3597 373 3063
Fragments arranged by size:
3597 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3174 4325 5096 6480 6748 1632
Size: 1542 1151 771 1384 268 1917
Fragments arranged by size:
1917 1542 1384 1151 771 268
AfeI AGC'GCT
Cuts at: 2646 2646
Size: 7033
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6836
AflIII A'CryG_T
Cuts at: 1203 5932 6909 1203
Size: 4729 977 1327
Fragments arranged by size:
4729 1327 977
AgeI A'CCGG_T
Cuts at: 5864 5864
Size: 7033
AhdI GACnn_n'nnGTC
Cuts at: 5762 5762
Size: 7033
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7033
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7033
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3572 3604 3572
Size: 32 7001
AlwNI CAG_nnn'CTG
Cuts at: 3122 4776 6348 3122
Size: 1654 1572 3807
Fragments arranged by size:
3807 1654 1572
ApaI G_GGCC'C
Cuts at: 4196 4196
Size: 7033
ApaLI G'TGCA_C
Cuts at: 2995 5090 6246 2995
Size: 2095 1156 3782
Fragments arranged by size:
3782 2095 1156
ApoI r'AATT_y
Cuts at: 1493 2555 4342 4568 4720 6829 1493
Size: 1062 1787 226 152 2109 1697
Fragments arranged by size:
2109 1787 1697 1062 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5587 160
Size: 872 4555 1606
Fragments arranged by size:
4555 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7033
AvaI C'yCGr_G
Cuts at: 1870 1943 3735 3790 3839 4358 1870
Size: 73 1792 55 49 519 4545
Fragments arranged by size:
4545 1792 519 73 55 49
BamHI G'GATC_C
Cuts at: 4363 4730 4363
Size: 367 6666
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2084 3730 4226 4354 5809
Size: 325 217 627 297 1646 496 128 1455
Cuts at: 5809 618
Size: 1842
Fragments arranged by size:
1842 1646 1455 627 496 325 297 217
128
BanII G_rGCy'C
Cuts at: 1391 3840 4196 4352 1391
Size: 2449 356 156 4072
Fragments arranged by size:
4072 2449 356 156
BbeI G_GCGC'C
Cuts at: 2088 2088
Size: 7033
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3149 3171 928
Size: 971 1250 22 4790
Fragments arranged by size:
4790 1250 971 22
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5224 5258 1903
Size: 34 3287 34 3678
Fragments arranged by size:
3678 3287 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4925 6135 4925
Size: 1210 5823
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7033
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5644 136
Size: 107 122 71 631 605 3972 1525
Fragments arranged by size:
3972 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7028 7028
Size: 7033
BlpI GC'TnA_GC
Cuts at: 2066 3893 4450 2066
Size: 1827 557 4649
Fragments arranged by size:
4649 1827 557
Bme1580I G_kGCm'C
Cuts at: 1790 2999 3735 4196 5094 6250 1790
Size: 1209 736 461 898 1156 2573
Fragments arranged by size:
2573 1209 1156 898 736 461
BmgBI CAC'GTC
Cuts at: 1318 3022 1318
Size: 1704 5329
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2256 4764 5722
Size: 416 53 83 399 24 839 2508 958
Cuts at: 5722 442
Size: 1753
Fragments arranged by size:
2508 1753 958 839 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7033
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2146 2146
Size: 7033
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5693 1239
Size: 4454 2579
Bpu10I CC'TnA_GC
Cuts at: 2580 2682 2580
Size: 102 6931
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2219 3809 5158 6023 6321 2219
Size: 1590 1349 865 298 2931
Fragments arranged by size:
2931 1590 1349 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3520 5696 882
Size: 617 2021 2176 2219
Fragments arranged by size:
2219 2176 2021 617
BsaAI yAC'GTr
Cuts at: 493 1204 3982 4824 493
Size: 711 2778 842 2702
Fragments arranged by size:
2778 2702 842 711
BsaBI GATnn'nnATC
Cuts at: 7027 7027
Size: 7033
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2085 3663 4207 4840
Size: 53 83 186 936 552 1578 544 633
Cuts at: 4840 5222 5857 275
Size: 382 635 1451
Fragments arranged by size:
1578 1451 936 635 633 552 544 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2431 3128 5466 5864 6138 6285 6696 2431
Size: 697 2338 398 274 147 411 2768
Fragments arranged by size:
2768 2338 697 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3572 3599 3602 3629 3986
Size: 30 165 30 2169 27 3 27 357
Cuts at: 3986 4016 1178
Size: 30 4195
Fragments arranged by size:
4195 2169 357 165 30 30 30 27
27 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2297 2627 2630 4165 1186
Size: 1111 330 3 1535 4054
Fragments arranged by size:
4054 1535 1111 330 3
BseYI C'CCAG_C
Cuts at: 4005 6236 6732 4005
Size: 2231 496 4306
Fragments arranged by size:
4306 2231 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2771 3039 3341 3392 3703 3962 4165
Size: 573 1035 268 302 51 311 259 203
Cuts at: 4165 1163
Size: 4031
Fragments arranged by size:
4031 1035 573 311 302 268 259 203
51
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3744 4483 5244 6272 1540
Size: 275 186 1743 739 761 1028 2301
Fragments arranged by size:
2301 1743 1028 761 739 275 186
BsiHKAI G_wGCw'C
Cuts at: 2999 3229 3309 3962 3987 4122 5094 5179 6250
Size: 230 80 653 25 135 972 85 1071
Cuts at: 6250 2999
Size: 3782
Fragments arranged by size:
3782 1071 972 653 230 135 85 80
25
BsmI GAATG_Cn'
Cuts at: 4544 4637 4544
Size: 93 6940
BsmBI CGTCTCn'nnnn_
Cuts at: 2800 2800
Size: 7033
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2999 3229 3309 3735 3840 3962 3987
Size: 399 1209 230 80 426 105 122 25
Cuts at: 3987 4122 4196 4352 5094 5179 6250 1391
Size: 135 74 156 742 85 1071 2174
Fragments arranged by size:
2174 1209 1071 742 426 399 230 156
135 122 105 85 80 74 25
BspEI T'CCGG_A
Cuts at: 3128 6696 3128
Size: 3568 3465
BspHI T'CATG_A
Cuts at: 4922 6652 4922
Size: 1730 5303
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3001 3504 4388 844
Size: 2157 503 884 3489
Fragments arranged by size:
3489 2157 884 503
BsrBI CCG'CTC
Cuts at: 1394 1823 4021 4920 1394
Size: 429 2198 899 3507
Fragments arranged by size:
3507 2198 899 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5528 5702 65
Size: 1183 4280 174 1396
Fragments arranged by size:
4280 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5677 5864 1700
Size: 110 3867 187 2869
Fragments arranged by size:
3867 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7033
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7033
BssSI C'ACGA_G
Cuts at: 5093 6105 5093
Size: 1012 6021
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7033
BstBI TT'CG_AA
Cuts at: 2931 5837 2931
Size: 2906 4127
BstEII G'GTnAC_C
Cuts at: 1184 3043 3460 3760 1184
Size: 1859 417 300 4457
Fragments arranged by size:
4457 1859 417 300
BstXI CCAn_nnnn'nTGG
Cuts at: 3772 3772
Size: 7033
BstYI r'GATC_y
Cuts at: 1972 3118 4363 4730 5115 5132 6584 6670 6682
Size: 1146 1245 367 385 17 1452 86 12
Cuts at: 6682 7028 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1245 1146 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3551 4188 3551
Size: 637 6396
BtgI C'CryG_G
Cuts at: 513 1065 2004 3610 3831 513
Size: 552 939 1606 221 3715
Fragments arranged by size:
3715 1606 939 552 221
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6294
Fragments arranged by size:
6294 683 56
BtsI GCAGTG_nn'
Cuts at: 4549 5343 5363 4549
Size: 794 20 6219
Fragments arranged by size:
6219 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7033
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6998
DraI TTT'AAA
Cuts at: 4337 4337
Size: 7033
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2179 6040 809
Size: 507 863 3861 1802
Fragments arranged by size:
3861 1802 863 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3535 3741 3963
Size: 54 1425 50 275 18 1705 206 222
Cuts at: 3963 4039 4480 5369 6998 8
Size: 76 441 889 1629 43
Fragments arranged by size:
1705 1629 1425 889 441 275 222 206
76 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3741 4480 1537
Size: 275 1929 739 4090
Fragments arranged by size:
4090 1929 739 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3006 4109 4963 1353
Size: 1653 1103 854 3423
Fragments arranged by size:
3423 1653 1103 854
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2788 2832 5620 5994 6140 387
Size: 1064 138 1199 44 2788 374 146 1280
Fragments arranged by size:
2788 1280 1199 1064 374 146 138 44
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3174 4325 5096 5693 6480 6748 1239
Size: 393 1542 1151 771 597 787 268 1524
Fragments arranged by size:
1542 1524 1151 787 771 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2895 2895
Size: 7033
EcoO109I rG'GnC_Cy
Cuts at: 2097 3487 3571 4192 4193 4233 2097
Size: 1390 84 621 1 40 4897
Fragments arranged by size:
4897 1390 621 84 40 1
EcoRI G'AATT_C
Cuts at: 2555 4342 4720 2555
Size: 1787 378 4868
Fragments arranged by size:
4868 1787 378
EcoRV GAT'ATC
Cuts at: 3327 3327
Size: 7033
FspI TGC'GCA
Cuts at: 2957 3878 5539 2957
Size: 921 1661 4451
Fragments arranged by size:
4451 1661 921
FspAI rTGC'GCAy
Cuts at: 2957 3878 2957
Size: 921 6112
HaeII r_GCGC'y
Cuts at: 1678 2088 2648 3429 3603 3641 6180 1678
Size: 410 560 781 174 38 2539 2531
Fragments arranged by size:
2539 2531 781 560 410 174 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2011 2043 2081 2113 2642
Size: 32 135 32 439 32 38 32 529
Cuts at: 2642 2674 3110 3142 3425 3457 3599 3631 3773
Size: 32 436 32 283 32 142 32 142
Cuts at: 3773 3805 3986 4018 5674 5706 5748 5780 1373
Size: 32 181 32 1656 32 42 32 2626
Fragments arranged by size:
2626 1656 529 439 436 283 181 142
142 135 42 38 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2172 4377 4623 669
Size: 988 281 234 2205 246 3079
Fragments arranged by size:
3079 2205 988 281 246 234
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7033
HpaI GTT'AAC
Cuts at: 4623 4623
Size: 7033
KasI G'GCGC_C
Cuts at: 2084 2084
Size: 7033
KpnI G_GTAC'C
Cuts at: 4358 4358
Size: 7033
MfeI C'AATT_G
Cuts at: 4632 4632
Size: 7033
MluI A'CGCG_T
Cuts at: 6909 6909
Size: 7033
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2571 6147 6331 1305
Size: 1266 3576 184 2007
Fragments arranged by size:
3576 2007 1266 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5211
Fragments arranged by size:
5211 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3278 3317 4991 5350 5509 518
Size: 648 2112 39 1674 359 159 2042
Fragments arranged by size:
2112 2042 1674 648 359 159 39
MspA1I CmG'CkG
Cuts at: 1606 3612 3697 3820 4176 4449 5126 6274 6519
Size: 2006 85 123 356 273 677 1148 245
Cuts at: 6519 1606
Size: 2120
Fragments arranged by size:
2120 2006 1148 677 356 273 245 123
85
NarI GG'CG_CC
Cuts at: 2085 2085
Size: 7033
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5542
Fragments arranged by size:
5542 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7033
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7033
NotI GC'GGCC_GC
Cuts at: 4480 4480
Size: 7033
NspI r_CATG'y
Cuts at: 1132 1229 4391 5936 1132
Size: 97 3162 1545 2229
Fragments arranged by size:
3162 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2209 1348
Size: 861 6172
PciI A'CATG_T
Cuts at: 5932 5932
Size: 7033
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7033
PfoI T'CCnGG_A
Cuts at: 3159 3159
Size: 7033
PmeI GTTT'AAAC
Cuts at: 4337 4337
Size: 7033
PmlI CAC'GTG
Cuts at: 3982 3982
Size: 7033
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3572 3604 4283 4315 5102 5134 6615 6647 3572
Size: 32 679 32 787 32 1481 32 3958
Fragments arranged by size:
3958 1481 787 679 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2097 3487 4233 2097
Size: 1390 746 4897
Fragments arranged by size:
4897 1390 746
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7033
PsiI TTA'TAA
Cuts at: 4603 4603
Size: 7033
PspOMI G'GGCC_C
Cuts at: 4192 4192
Size: 7033
PstI C_TGCA'G
Cuts at: 830 3012 3702 4385 830
Size: 2182 690 683 3478
Fragments arranged by size:
3478 2182 690 683
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7033
PvuII CAG'CTG
Cuts at: 3820 3820
Size: 7033
SacII CC_GC'GG
Cuts at: 3613 3613
Size: 7033
SalI G'TCGA_C
Cuts at: 1936 4375 1936
Size: 2439 4594
SanDI GG'GwC_CC
Cuts at: 4233 4233
Size: 7033
SapI GCTCTTCn'nnn_
Cuts at: 4109 4109
Size: 7033
SbfI CC_TGCA'GG
Cuts at: 3012 4385 3012
Size: 1373 5660
ScaI AGT'ACT
Cuts at: 5281 6993 5281
Size: 1712 5321
SexAI A'CCwGG_T
Cuts at: 3756 4071 3756
Size: 315 6718
SfcI C'TryA_G
Cuts at: 826 1045 3008 3698 4381 5516 6197 6388 826
Size: 219 1963 690 683 1135 681 191 1471
Fragments arranged by size:
1963 1471 1135 690 683 681 219 191
SfoI GGC'GCC
Cuts at: 2086 2086
Size: 7033
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7033
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7033
SmaI CCC'GGG
Cuts at: 1872 3737 3841 4360 1872
Size: 1865 104 519 4545
Fragments arranged by size:
4545 1865 519 104
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2234 3824 5137 6038 6300 820
Size: 197 926 291 1590 1313 901 262 1553
Fragments arranged by size:
1590 1553 1313 926 901 291 262 197
SnaBI TAC'GTA
Cuts at: 493 4824 493
Size: 4331 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7033
SphI G_CATG'C
Cuts at: 1132 4391 1132
Size: 3259 3774
SrfI GCCC'GGGC
Cuts at: 3841 3841
Size: 7033
SspI AAT'ATT
Cuts at: 5 52 2352 4957 5
Size: 47 2300 2605 2081
Fragments arranged by size:
2605 2300 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7033
StyI C'CwwG_G
Cuts at: 513 1065 2004 3210 3290 3765 4472 513
Size: 552 939 1206 80 475 707 3074
Fragments arranged by size:
3074 1206 939 707 552 475 80
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3374 5060 5077 5230 5415 5860 325
Size: 1255 1794 1686 17 153 185 445 1498
Fragments arranged by size:
1794 1686 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5279 6991 96
Size: 274 80 33 51 1443 3302 1712 138
Fragments arranged by size:
3302 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6993 7025 6993
Size: 32 7001
Tth111I GACn'n_nGTC
Cuts at: 1316 3049 4237 1316
Size: 1733 1188 4112
Fragments arranged by size:
4112 1733 1188
XbaI T'CTAG_A
Cuts at: 4369 4369
Size: 7033
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3919 3919
Size: 7033
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7033
XmaI C'CCGG_G
Cuts at: 1870 3735 3839 4358 1870
Size: 1865 104 519 4545
Fragments arranged by size:
4545 1865 519 104
XmnI GAAnn'nnTTC
Cuts at: 2352 2418 3168 5162 2352
Size: 66 750 1994 4223
Fragments arranged by size:
4223 1994 750 66
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4841 276
Size: 53 83 186 936 3307 2468
Fragments arranged by size:
3307 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I DraIII
EcoICRI FalI FseI NaeI NgoMIV NruI NsiI PacI
PspXI PsrI RsrII SacI SfiI SwaI