Restriction map for
pFN21AA1619
(Circular) (Six-base) MAPSORT of: pFN21AA1619.seq Check: 9920 from: 1 to: 7234
pFN21AA1619 7234 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5044 278
Size: 53 83 186 936 3508 2468
Fragments arranged by size:
3508 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4577 1937
Size: 2640 4594
Acc65I G'GTAC_C
Cuts at: 4555 4555
Size: 7234
AclI AA'CG_TT
Cuts at: 1563 5361 5734 1563
Size: 3798 373 3063
Fragments arranged by size:
3798 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3746 5297 6681 6949 1632
Size: 2114 1551 1384 268 1917
Fragments arranged by size:
2114 1917 1551 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7037
AflIII A'CryG_T
Cuts at: 1203 3245 6133 7110 1203
Size: 2042 2888 977 1327
Fragments arranged by size:
2888 2042 1327 977
AgeI A'CCGG_T
Cuts at: 6065 6065
Size: 7234
AhdI GACnn_n'nnGTC
Cuts at: 5963 5963
Size: 7234
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7234
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4465 1184
Size: 3281 3953
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2645 2677 2645
Size: 32 7202
AlwNI CAG_nnn'CTG
Cuts at: 2051 4977 6549 2051
Size: 2926 1572 2736
Fragments arranged by size:
2926 2736 1572
ApaI G_GGCC'C
Cuts at: 4449 4449
Size: 7234
ApaLI G'TGCA_C
Cuts at: 5291 6447 5291
Size: 1156 6078
ApoI r'AATT_y
Cuts at: 1493 2564 4543 4769 4921 7030 1493
Size: 1071 1979 226 152 2109 1697
Fragments arranged by size:
2109 1979 1697 1071 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5788 160
Size: 872 4756 1606
Fragments arranged by size:
4756 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7234
AvaI C'yCGr_G
Cuts at: 1870 1943 2220 3564 3792 4559 1870
Size: 73 277 1344 228 767 4545
Fragments arranged by size:
4545 1344 767 277 228 73
BamHI G'GATC_C
Cuts at: 3366 4564 4931 3366
Size: 1198 367 5669
Fragments arranged by size:
5669 1198 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2225 3109 3593 4027 4140
Size: 325 217 627 438 884 484 434 113
Cuts at: 4140 4555 6010 618
Size: 415 1455 1842
Fragments arranged by size:
1842 1455 884 627 484 438 434 415
325 217 113
BanII G_rGCy'C
Cuts at: 1391 2133 2221 2269 2756 3120 3392 3493 4306
Size: 742 88 48 487 364 272 101 813
Cuts at: 4306 4449 4529 4553 1391
Size: 143 80 24 4072
Fragments arranged by size:
4072 813 742 487 364 272 143 101
88 80 48 24
BbeI G_GCGC'C
Cuts at: 4144 4144
Size: 7234
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2996 3734 928
Size: 971 1097 738 4428
Fragments arranged by size:
4428 1097 971 738
BbvCI CC'TCA_GC
Cuts at: 2033 2197 2445 3004 2033
Size: 164 248 559 6263
Fragments arranged by size:
6263 559 248 164
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5425 5459 1903
Size: 34 3488 34 3678
Fragments arranged by size:
3678 3488 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3697 5126 6336 3697
Size: 1429 1210 4595
Fragments arranged by size:
4595 1429 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7234
BfrBI ATG'CAT
Cuts at: 2399 2635 2399
Size: 236 6998
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2801 4175 4269
Size: 107 122 71 631 605 1129 1374 94
Cuts at: 4269 4378 5845 136
Size: 109 1467 1525
Fragments arranged by size:
1525 1467 1374 1129 631 605 122 109
107 94 71
BglII A'GATC_T
Cuts at: 7229 7229
Size: 7234
BlpI GC'TnA_GC
Cuts at: 2975 4344 4651 2975
Size: 1369 307 5558
Fragments arranged by size:
5558 1369 307
Bme1580I G_kGCm'C
Cuts at: 1790 2230 3243 3353 4021 4449 5295 6451 1790
Size: 440 1013 110 668 428 846 1156 2573
Fragments arranged by size:
2573 1156 1013 846 668 440 428 110
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7234
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2293 3430 4169
Size: 416 53 83 399 24 876 1137 739
Cuts at: 4169 4965 5923 442
Size: 796 958 1753
Fragments arranged by size:
1753 1137 958 876 796 739 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 4058 1056
Size: 3002 4232
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2212 2770 2212
Size: 558 6676
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2207 3017 3505 4245 4265 4286 5894 1239
Size: 968 810 488 740 20 21 1608 2579
Fragments arranged by size:
2579 1608 968 810 740 488 21 20
Bpu10I CC'TnA_GC
Cuts at: 2033 2197 2445 3004 3114 3827 3891 2033
Size: 164 248 559 110 713 64 5376
Fragments arranged by size:
5376 713 559 248 164 110 64
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2926 3251 5359 6224 6522 2926
Size: 325 2108 865 298 3638
Fragments arranged by size:
3638 2108 865 325 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2071 2814 3561 5897 882
Size: 617 572 743 747 2336 2219
Fragments arranged by size:
2336 2219 747 743 617 572
BsaAI yAC'GTr
Cuts at: 493 1204 3248 5025 493
Size: 711 2044 1777 2702
Fragments arranged by size:
2702 2044 1777 711
BsaBI GATnn'nnATC
Cuts at: 7228 7228
Size: 7234
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4141 5041 5423 6058
Size: 53 83 186 936 2608 900 382 635
Cuts at: 6058 275
Size: 1451
Fragments arranged by size:
2608 1451 936 900 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2568 5667 6065 6339 6486 6897 2568
Size: 3099 398 274 147 411 2905
Fragments arranged by size:
3099 2905 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2591 2621 2645 2675 3422
Size: 30 165 30 1188 30 24 30 747
Cuts at: 3422 3452 1178
Size: 30 4960
Fragments arranged by size:
4960 1188 747 165 30 30 30 30
30 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2018 2021 2204 2489 2636 2762 4087 4094
Size: 832 3 183 285 147 126 1325 7
Cuts at: 4094 1186
Size: 4326
Fragments arranged by size:
4326 1325 832 285 183 147 126 7
3
BseYI C'CCAG_C
Cuts at: 2503 3087 3582 3988 4256 4306 4373 6437 6933
Size: 584 495 406 268 50 67 2064 496
Cuts at: 6933 2503
Size: 2804
Fragments arranged by size:
2804 2064 584 496 495 406 268 67
50
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2451 2715 1163
Size: 573 715 264 5682
Fragments arranged by size:
5682 715 573 264
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4684 5445 6473 1540
Size: 275 186 2683 761 1028 2301
Fragments arranged by size:
2683 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2133 2981 3744 3897 4529 5295 5380 6451 2133
Size: 848 763 153 632 766 85 1071 2916
Fragments arranged by size:
2916 1071 848 766 763 632 153 85
BsmI GAATG_Cn'
Cuts at: 2574 4745 4838 2574
Size: 2171 93 4970
Fragments arranged by size:
4970 2171 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2882 2882
Size: 7234
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2133 2221 2230 2269 2756 2981 3120
Size: 399 343 88 9 39 487 225 139
Cuts at: 3120 3243 3353 3392 3493 3744 3897 4021 4306
Size: 123 110 39 101 251 153 124 285
Cuts at: 4306 4449 4529 4553 5295 5380 6451 1391
Size: 143 80 24 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 487 399 343 285 251
225 153 143 139 124 123 110 101
88 85 80 39 39 24 9
BspEI T'CCGG_A
Cuts at: 2568 6897 2568
Size: 4329 2905
BspHI T'CATG_A
Cuts at: 5123 6853 5123
Size: 1730 5504
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3345 4589 844
Size: 2501 1244 3489
Fragments arranged by size:
3489 2501 1244
BsrBI CCG'CTC
Cuts at: 1394 1823 3735 5121 1394
Size: 429 1912 1386 3507
Fragments arranged by size:
3507 1912 1386 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3146 4059 5729 5903 65
Size: 1183 1898 913 1670 174 1396
Fragments arranged by size:
1898 1670 1396 1183 913 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4339 4377 5878 6065 1700
Size: 110 2529 38 1501 187 2869
Fragments arranged by size:
2869 2529 1501 187 110 38
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7234
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7234
BssSI C'ACGA_G
Cuts at: 2672 2805 3130 4505 5294 6306 2672
Size: 133 325 1375 789 1012 3600
Fragments arranged by size:
3600 1375 1012 789 325 133
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7234
BstBI TT'CG_AA
Cuts at: 6038 6038
Size: 7234
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7234
BstXI CCAn_nnnn'nTGG
Cuts at: 2637 2698 4223 4360 2637
Size: 61 1525 137 5511
Fragments arranged by size:
5511 1525 137 61
BstYI r'GATC_y
Cuts at: 1972 2277 2838 3033 3366 4184 4564 4931 5316
Size: 305 561 195 333 818 380 367 385
Cuts at: 5316 5333 6785 6871 6883 7229 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 818 561 385 380 367 346
333 305 195 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2509 513
Size: 552 1444 5238
Fragments arranged by size:
5238 1444 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3846 487
Size: 683 56 2620 3875
Fragments arranged by size:
3875 2620 683 56
BtsI GCAGTG_nn'
Cuts at: 2069 2177 2703 2906 4367 4750 5544 5564 2069
Size: 108 526 203 1461 383 794 20 3739
Fragments arranged by size:
3739 1461 794 526 383 203 108 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7234
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7199
DraI TTT'AAA
Cuts at: 4538 4538
Size: 7234
DraIII CAC_nnn'GTG
Cuts at: 2791 2912 2985 2791
Size: 121 73 7040
Fragments arranged by size:
7040 121 73
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6241 809
Size: 507 4925 1802
Fragments arranged by size:
4925 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3971 4681 5570
Size: 54 1425 50 275 18 2141 710 889
Cuts at: 5570 7199 8
Size: 1629 43
Fragments arranged by size:
2141 1629 1425 889 710 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4681 1537
Size: 275 2869 4090
Fragments arranged by size:
4090 2869 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2121 3405 5164 1353
Size: 768 1284 1759 3423
Fragments arranged by size:
3423 1759 1284 768
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2576 5821 6195 6341 387
Size: 1064 138 987 3245 374 146 1280
Fragments arranged by size:
3245 1280 1064 987 374 146 138
EcoICRI GAG'CTC
Cuts at: 2131 4527 2131
Size: 2396 4838
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2207 3017 3505 3746 4245 4265 4286
Size: 393 575 810 488 241 499 20 21
Cuts at: 4286 5297 5894 6681 6949 1239
Size: 1011 597 787 268 1524
Fragments arranged by size:
1524 1011 810 787 597 575 499 488
393 268 241 21 20
EcoNI CCTnn'n_nnAGG
Cuts at: 2200 2200
Size: 7234
EcoO109I rG'GnC_Cy
Cuts at: 2063 2139 3053 3586 3633 4261 4445 4446 2063
Size: 76 914 533 47 628 184 1 4851
Fragments arranged by size:
4851 914 628 533 184 76 47 1
EcoRI G'AATT_C
Cuts at: 2564 4543 4921 2564
Size: 1979 378 4877
Fragments arranged by size:
4877 1979 378
FspI TGC'GCA
Cuts at: 3152 3607 5740 3152
Size: 455 2133 4646
Fragments arranged by size:
4646 2133 455
HaeII r_GCGC'y
Cuts at: 1678 3088 4144 6381 1678
Size: 1410 1056 2237 2531
Fragments arranged by size:
2531 2237 1410 1056
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3170 3202 3422 3454 3664
Size: 32 135 32 1598 32 220 32 210
Cuts at: 3664 3696 3764 3796 4511 4543 5875 5907 5949
Size: 32 68 32 715 32 1332 32 42
Cuts at: 5949 5981 1373
Size: 32 2626
Fragments arranged by size:
2626 1598 1332 715 220 210 135 68
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4578 4824 669
Size: 988 281 2640 246 3079
Fragments arranged by size:
3079 2640 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7234
HpaI GTT'AAC
Cuts at: 4824 4824
Size: 7234
KasI G'GCGC_C
Cuts at: 4140 4140
Size: 7234
KpnI G_GTAC'C
Cuts at: 4559 4559
Size: 7234
MfeI C'AATT_G
Cuts at: 4833 4833
Size: 7234
MluI A'CGCG_T
Cuts at: 7110 7110
Size: 7234
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3807 6348 6532 1305
Size: 2502 2541 184 2007
Fragments arranged by size:
2541 2502 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5412
Fragments arranged by size:
5412 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2394 2958 5192 5551 5710 518
Size: 648 1228 564 2234 359 159 2042
Fragments arranged by size:
2234 2042 1228 648 564 359 159
MspA1I CmG'CkG
Cuts at: 1606 2374 3356 3525 4024 4275 4650 5327 6475
Size: 768 982 169 499 251 375 677 1148
Cuts at: 6475 6720 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 982 768 677 499 375 251
245 169
NaeI GCC'GGC
Cuts at: 4379 4379
Size: 7234
NarI GG'CG_CC
Cuts at: 4141 4141
Size: 7234
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6682
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7234
NgoMIV G'CCGG_C
Cuts at: 4377 4377
Size: 7234
NheI G'CTAG_C
Cuts at: 1052 4054 1052
Size: 3002 4232
NotI GC'GGCC_GC
Cuts at: 4681 4681
Size: 7234
NsiI A_TGCA'T
Cuts at: 2401 2637 2401
Size: 236 6998
NspI r_CATG'y
Cuts at: 1132 1229 2403 4592 6137 1132
Size: 97 1174 2189 1545 2229
Fragments arranged by size:
2229 2189 1545 1174 97
PasI CC'CwG_GG
Cuts at: 1348 3637 4292 1348
Size: 2289 655 4290
Fragments arranged by size:
4290 2289 655
PciI A'CATG_T
Cuts at: 6133 6133
Size: 7234
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2510 3442 1108
Size: 1402 932 4900
Fragments arranged by size:
4900 1402 932
PfoI T'CCnGG_A
Cuts at: 3195 4222 4479 3195
Size: 1027 257 5950
Fragments arranged by size:
5950 1027 257
PmeI GTTT'AAAC
Cuts at: 4538 4538
Size: 7234
PmlI CAC'GTG
Cuts at: 3248 3248
Size: 7234
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2645 2677 5303 5335 6816 6848 2645
Size: 32 2626 32 1481 32 3031
Fragments arranged by size:
3031 2626 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2063 2139 3053 3586 3633 2063
Size: 76 914 533 47 5664
Fragments arranged by size:
5664 914 533 76 47
PshAI GACnn'nnGTC
Cuts at: 1613 3305 1613
Size: 1692 5542
PsiI TTA'TAA
Cuts at: 4804 4804
Size: 7234
PspOMI G'GGCC_C
Cuts at: 4445 4445
Size: 7234
PstI C_TGCA'G
Cuts at: 830 2056 2373 2847 2924 4203 4586 830
Size: 1226 317 474 77 1279 383 3478
Fragments arranged by size:
3478 1279 1226 474 383 317 77
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7234
PvuII CAG'CTG
Cuts at: 3356 3525 4024 4275 3356
Size: 169 499 251 6315
Fragments arranged by size:
6315 499 251 169
SacI G_AGCT'C
Cuts at: 2133 4529 2133
Size: 2396 4838
SalI G'TCGA_C
Cuts at: 1936 4576 1936
Size: 2640 4594
SanDI GG'GwC_CC
Cuts at: 2139 3586 2139
Size: 1447 5787
SapI GCTCTTCn'nnn_
Cuts at: 2121 2121
Size: 7234
SbfI CC_TGCA'GG
Cuts at: 4586 4586
Size: 7234
ScaI AGT'ACT
Cuts at: 5482 7194 5482
Size: 1712 5522
SfcI C'TryA_G
Cuts at: 826 1045 2052 2369 2843 2920 3955 4199 4582
Size: 219 1007 317 474 77 1035 244 383
Cuts at: 4582 5717 6398 6589 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1035 1007 681 474 383 317
244 219 191 77
SfoI GGC'GCC
Cuts at: 4142 4142
Size: 7234
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7234
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7234
SmaI CCC'GGG
Cuts at: 1872 3794 4561 1872
Size: 1922 767 4545
Fragments arranged by size:
4545 1922 767
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2941 3266 5338 6239 6501 820
Size: 197 926 998 325 2072 901 262 1553
Fragments arranged by size:
2072 1553 998 926 901 325 262 197
SnaBI TAC'GTA
Cuts at: 493 5025 493
Size: 4532 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7234
SphI G_CATG'C
Cuts at: 1132 4592 1132
Size: 3460 3774
SrfI GCCC'GGGC
Cuts at: 3794 3794
Size: 7234
SspI AAT'ATT
Cuts at: 5 52 5158 5
Size: 47 5106 2081
Fragments arranged by size:
5106 2081 47
StuI AGG'CCT
Cuts at: 1517 2195 2450 2523 2541 3002 1517
Size: 678 255 73 18 461 5749
Fragments arranged by size:
5749 678 461 255 73 18
StyI C'CwwG_G
Cuts at: 513 1065 3157 4673 513
Size: 552 2092 1516 3074
Fragments arranged by size:
3074 2092 1516 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2616 2690 3231 3611 5261 5278 5431
Size: 1255 1036 74 541 380 1650 17 153
Cuts at: 5431 5616 6061 325
Size: 185 445 1498
Fragments arranged by size:
1650 1498 1255 1036 541 445 380 185
153 74 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2718 5480 7192
Size: 274 80 33 51 1443 741 2762 1712
Cuts at: 7192 96
Size: 138
Fragments arranged by size:
2762 1712 1443 741 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2591 2623 4176 4208 7194 7226 2591
Size: 32 1553 32 2986 32 2599
Fragments arranged by size:
2986 2599 1553 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7234
XbaI T'CTAG_A
Cuts at: 2084 4570 2084
Size: 2486 4748
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4289 4360 4289
Size: 71 7163
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7234
XmaI C'CCGG_G
Cuts at: 1870 3792 4559 1870
Size: 1922 767 4545
Fragments arranged by size:
4545 1922 767
XmnI GAAnn'nnTTC
Cuts at: 5363 5363
Size: 7234
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5042 276
Size: 53 83 186 936 3508 2468
Fragments arranged by size:
3508 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BsiWI BstZ17I Bsu36I
EcoRV FalI FseI FspAI NruI PacI PspXI PsrI
RsrII SacII SexAI SfiI SwaI