Restriction map for
pFN21AA1592
(Circular) (Six-base) MAPSORT of: pFN21AA1592.seq Check: 6024 from: 1 to: 6598
pFN21AA1592 6598 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3591 4408 278
Size: 53 83 186 936 2055 817 2468
Fragments arranged by size:
2468 2055 936 817 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3941 1937
Size: 2004 4594
Acc65I G'GTAC_C
Cuts at: 3919 3919
Size: 6598
AclI AA'CG_TT
Cuts at: 1563 4725 5098 1563
Size: 3162 373 3063
Fragments arranged by size:
3162 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2075 3649 4661 6045 6313 1632
Size: 443 1574 1012 1384 268 1917
Fragments arranged by size:
1917 1574 1384 1012 443 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6401
AflIII A'CryG_T
Cuts at: 1203 3111 5497 6474 1203
Size: 1908 2386 977 1327
Fragments arranged by size:
2386 1908 1327 977
AgeI A'CCGG_T
Cuts at: 5429 5429
Size: 6598
AhdI GACnn_n'nnGTC
Cuts at: 2815 5327 2815
Size: 2512 4086
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6598
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6598
AlwNI CAG_nnn'CTG
Cuts at: 2440 4341 5913 2440
Size: 1901 1572 3125
Fragments arranged by size:
3125 1901 1572
ApaI G_GGCC'C
Cuts at: 2035 2049 2035
Size: 14 6584
ApaLI G'TGCA_C
Cuts at: 2023 2918 3778 4655 5811 2023
Size: 895 860 877 1156 2810
Fragments arranged by size:
2810 1156 895 877 860
ApoI r'AATT_y
Cuts at: 1493 2492 3907 4133 4285 6394 1493
Size: 999 1415 226 152 2109 1697
Fragments arranged by size:
2109 1697 1415 999 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5152 160
Size: 872 4120 1606
Fragments arranged by size:
4120 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6598
AvaI C'yCGr_G
Cuts at: 1870 1943 2028 2183 3923 1870
Size: 73 85 155 1740 4545
Fragments arranged by size:
4545 1740 155 85 73
AvrII C'CTAG_G
Cuts at: 2314 2314
Size: 6598
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3296 3329 3296
Size: 33 6565
BamHI G'GATC_C
Cuts at: 3928 4295 3928
Size: 367 6231
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2234 2468 2663 3473 3919
Size: 325 217 627 447 234 195 810 446
Cuts at: 3919 5374 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 810 627 447 446 325 234
217 195
BanII G_rGCy'C
Cuts at: 1391 2035 2049 2274 2622 2649 3198 3917 1391
Size: 644 14 225 348 27 549 719 4072
Fragments arranged by size:
4072 719 644 549 348 225 27 14
BbeI G_GCGC'C
Cuts at: 3477 3477
Size: 6598
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2814 2920 928
Size: 971 915 106 4606
Fragments arranged by size:
4606 971 915 106
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4789 4823 1903
Size: 34 2852 34 3678
Fragments arranged by size:
3678 2852 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4490 5700 4490
Size: 1210 5388
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6598
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2044 2119 2278
Size: 107 122 71 631 605 372 75 159
Cuts at: 2278 5209 136
Size: 2931 1525
Fragments arranged by size:
2931 1525 631 605 372 159 122 107
75 71
BglII A'GATC_T
Cuts at: 6593 6593
Size: 6598
BlpI GC'TnA_GC
Cuts at: 3556 4015 3556
Size: 459 6139
Bme1580I G_kGCm'C
Cuts at: 1790 2027 2035 2049 2668 2922 3679 3782 4659
Size: 237 8 14 619 254 757 103 877
Cuts at: 4659 5815 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 877 757 619 254 237 103
14 8
BmgBI CAC'GTC
Cuts at: 1318 3067 1318
Size: 1749 4849
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4329 5287 442
Size: 416 53 83 399 24 2912 958 1753
Fragments arranged by size:
2912 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6598
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3877 3877
Size: 6598
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2696 2978 3080 5258 1239
Size: 1457 282 102 2178 2579
Fragments arranged by size:
2579 2178 1457 282 102
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2870 3293 3320 4723 5588 5886 2870
Size: 423 27 1403 865 298 3582
Fragments arranged by size:
3582 1403 865 423 298 27
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3262 3827 5261 882
Size: 617 1763 565 1434 2219
Fragments arranged by size:
2219 1763 1434 617 565
BsaAI yAC'GTr
Cuts at: 493 1204 4389 493
Size: 711 3185 2702
Fragments arranged by size:
3185 2702 711
BsaBI GATnn'nnATC
Cuts at: 6592 6592
Size: 6598
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3474 3588 4405 4787
Size: 53 83 186 936 1941 114 817 382
Cuts at: 4787 5422 275
Size: 635 1451
Fragments arranged by size:
1941 1451 936 817 635 382 186 114
83 53
BsaWI w'CCGG_w
Cuts at: 5031 5429 5703 5850 6261 5031
Size: 398 274 147 411 5368
Fragments arranged by size:
5368 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3863 3893 1178
Size: 30 165 30 2460 30 3883
Fragments arranged by size:
3883 2460 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2108 2383 2657 2975 3089 3212 1186
Size: 922 275 274 318 114 123 4572
Fragments arranged by size:
4572 922 318 275 274 123 114
BseYI C'CCAG_C
Cuts at: 2034 2706 3529 5801 6297 2034
Size: 672 823 2272 496 2335
Fragments arranged by size:
2335 2272 823 672 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2108 2243 2860 3017 3854 1163
Size: 573 372 135 617 157 837 3907
Fragments arranged by size:
3907 837 617 573 372 157 135
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3399 3523 4048 4809 5837 1540
Size: 275 186 1398 124 525 761 1028 2301
Fragments arranged by size:
2301 1398 1028 761 525 275 186 124
BsiHKAI G_wGCw'C
Cuts at: 2027 2447 2649 2922 3198 3351 3782 4659 4744
Size: 420 202 273 276 153 431 877 85
Cuts at: 4744 5815 2027
Size: 1071 2810
Fragments arranged by size:
2810 1071 877 431 420 276 273 202
153 85
BsmI GAATG_Cn'
Cuts at: 3895 4109 4202 3895
Size: 214 93 6291
Fragments arranged by size:
6291 214 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3461 3595 3461
Size: 134 6464
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2027 2035 2049 2274 2447 2622 2649
Size: 399 237 8 14 225 173 175 27
Cuts at: 2649 2668 2922 3198 3351 3679 3782 3917 4659
Size: 19 254 276 153 328 103 135 742
Cuts at: 4659 4744 5815 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 328 276 254 237
225 175 173 153 135 103 85 27
19 14 8
BspEI T'CCGG_A
Cuts at: 6261 6261
Size: 6598
BspHI T'CATG_A
Cuts at: 2700 2724 4487 6217 2700
Size: 24 1763 1730 3081
Fragments arranged by size:
3081 1763 1730 24
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3953 844
Size: 3109 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3233 3555 4485 1394
Size: 429 1410 322 930 3507
Fragments arranged by size:
3507 1410 930 429 322
BsrDI GCAATG_nn'
Cuts at: 65 1248 5093 5267 65
Size: 1183 3845 174 1396
Fragments arranged by size:
3845 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3394 5242 5429 1700
Size: 110 1584 1848 187 2869
Fragments arranged by size:
2869 1848 1584 187 110
BsrGI T'GTAC_A
Cuts at: 96 3114 3378 96
Size: 3018 264 3316
Fragments arranged by size:
3316 3018 264
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6598
BssSI C'ACGA_G
Cuts at: 2633 3680 3884 4658 5670 2633
Size: 1047 204 774 1012 3561
Fragments arranged by size:
3561 1047 1012 774 204
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6598
BstBI TT'CG_AA
Cuts at: 2805 5402 2805
Size: 2597 4001
BstEII G'GTnAC_C
Cuts at: 1184 3023 3052 1184
Size: 1839 29 4730
Fragments arranged by size:
4730 1839 29
BstXI CCAn_nnnn'nTGG
Cuts at: 3790 3790
Size: 6598
BstYI r'GATC_y
Cuts at: 1972 2424 3093 3291 3928 4295 4680 4697 6149
Size: 452 669 198 637 367 385 17 1452
Cuts at: 6149 6235 6247 6593 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 669 637 452 385 367 346
198 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2433 2433
Size: 6598
BtgI C'CryG_G
Cuts at: 513 1065 2205 513
Size: 552 1140 4906
Fragments arranged by size:
4906 1140 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2751 3221 487
Size: 683 56 1525 470 3864
Fragments arranged by size:
3864 1525 683 470 56
BtsI GCAGTG_nn'
Cuts at: 2075 4114 4908 4928 2075
Size: 2039 794 20 3745
Fragments arranged by size:
3745 2039 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6598
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6563
DraI TTT'AAA
Cuts at: 3902 3902
Size: 6598
DraIII CAC_nnn'GTG
Cuts at: 2917 2917
Size: 6598
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3668 5605 809
Size: 507 2352 1937 1802
Fragments arranged by size:
2352 1937 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2988 3228 3396
Size: 54 1425 50 275 18 1158 240 168
Cuts at: 3396 3801 4045 4934 6563 8
Size: 405 244 889 1629 43
Fragments arranged by size:
1629 1425 1158 889 405 275 244 240
168 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3396 4045 1537
Size: 275 1584 649 4090
Fragments arranged by size:
4090 1584 649 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2087 3849 4528 1353
Size: 734 1762 679 3423
Fragments arranged by size:
3423 1762 734 679
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5185 5559 5705 387
Size: 1064 138 3596 374 146 1280
Fragments arranged by size:
3596 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2647 3196 2647
Size: 549 6049
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2075 2696 2978 3080 3649 4661 5258
Size: 393 443 621 282 102 569 1012 597
Cuts at: 5258 6045 6313 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1012 787 621 597 569 443 393
282 268 102
EcoNI CCTnn'n_nnAGG
Cuts at: 2432 2432
Size: 6598
EcoO109I rG'GnC_Cy
Cuts at: 2046 2437 3040 2046
Size: 391 603 5604
Fragments arranged by size:
5604 603 391
EcoRI G'AATT_C
Cuts at: 3907 4285 3907
Size: 378 6220
EcoRV GAT'ATC
Cuts at: 2698 3282 2698
Size: 584 6014
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3181 3181
Size: 6598
FspI TGC'GCA
Cuts at: 2216 5104 2216
Size: 2888 3710
HaeII r_GCGC'y
Cuts at: 1678 2254 3477 5745 1678
Size: 576 1223 2268 2531
Fragments arranged by size:
2531 2268 1223 576
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2057 2089 2416 2448 2801
Size: 32 135 32 485 32 327 32 353
Cuts at: 2801 2833 2881 2913 3826 3858 5239 5271 5313
Size: 32 48 32 913 32 1381 32 42
Cuts at: 5313 5345 1373
Size: 32 2626
Fragments arranged by size:
2626 1381 913 485 353 327 135 48
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2332 3942 4188 669
Size: 988 281 394 1610 246 3079
Fragments arranged by size:
3079 1610 988 394 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6598
HpaI GTT'AAC
Cuts at: 4188 4188
Size: 6598
KasI G'GCGC_C
Cuts at: 3473 3473
Size: 6598
KpnI G_GTAC'C
Cuts at: 3923 3923
Size: 6598
MfeI C'AATT_G
Cuts at: 4197 4197
Size: 6598
MluI A'CGCG_T
Cuts at: 6474 6474
Size: 6598
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2745 2925 3069 3132 3543 5712 5896 1305
Size: 1440 180 144 63 411 2169 184 2007
Fragments arranged by size:
2169 2007 1440 411 184 180 144 63
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2990 10
Size: 54 1425 343 1158 3618
Fragments arranged by size:
3618 1425 1158 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2384 2798 3788 3888 4556 4915 5074
Size: 648 1218 414 990 100 668 359 159
Cuts at: 5074 518
Size: 2042
Fragments arranged by size:
2042 1218 990 668 648 414 359 159
100
MspA1I CmG'CkG
Cuts at: 1606 4014 4691 5839 6084 1606
Size: 2408 677 1148 245 2120
Fragments arranged by size:
2408 2120 1148 677 245
NaeI GCC'GGC
Cuts at: 3396 3396
Size: 6598
NarI GG'CG_CC
Cuts at: 3474 3474
Size: 6598
NcoI C'CATG_G
Cuts at: 513 1065 2205 513
Size: 552 1140 4906
Fragments arranged by size:
4906 1140 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6598
NgoMIV G'CCGG_C
Cuts at: 3394 3394
Size: 6598
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6598
NotI GC'GGCC_GC
Cuts at: 4045 4045
Size: 6598
NspI r_CATG'y
Cuts at: 1132 1229 3115 3956 5501 1132
Size: 97 1886 841 1545 2229
Fragments arranged by size:
2229 1886 1545 841 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6598
PciI A'CATG_T
Cuts at: 3111 5497 3111
Size: 2386 4212
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6598
PfoI T'CCnGG_A
Cuts at: 2541 2712 2745 3096 2541
Size: 171 33 351 6043
Fragments arranged by size:
6043 351 171 33
PmeI GTTT'AAAC
Cuts at: 3902 3902
Size: 6598
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4667 4699 6180 6212 4667
Size: 32 1481 32 5053
Fragments arranged by size:
5053 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3040 3040
Size: 6598
PshAI GACnn'nnGTC
Cuts at: 1613 3509 1613
Size: 1896 4702
PsiI TTA'TAA
Cuts at: 4168 4168
Size: 6598
PspOMI G'GGCC_C
Cuts at: 2031 2045 2031
Size: 14 6584
PstI C_TGCA'G
Cuts at: 830 3423 3601 3950 830
Size: 2593 178 349 3478
Fragments arranged by size:
3478 2593 349 178
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6598
SacI G_AGCT'C
Cuts at: 2649 3198 2649
Size: 549 6049
SalI G'TCGA_C
Cuts at: 1936 3940 1936
Size: 2004 4594
SapI GCTCTTCn'nnn_
Cuts at: 2087 2087
Size: 6598
SbfI CC_TGCA'GG
Cuts at: 3423 3950 3423
Size: 527 6071
ScaI AGT'ACT
Cuts at: 3359 4846 6558 3359
Size: 1487 1712 3399
Fragments arranged by size:
3399 1712 1487
SfcI C'TryA_G
Cuts at: 826 1045 2688 3419 3597 3946 5081 5762 5953
Size: 219 1643 731 178 349 1135 681 191
Cuts at: 5953 826
Size: 1471
Fragments arranged by size:
1643 1471 1135 731 681 349 219 191
178
SfoI GGC'GCC
Cuts at: 3475 3475
Size: 6598
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6598
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6598
SmaI CCC'GGG
Cuts at: 1872 2030 3925 1872
Size: 158 1895 4545
Fragments arranged by size:
4545 1895 158
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2885 3308 3335 4702 5603 5865
Size: 197 926 942 423 27 1367 901 262
Cuts at: 5865 820
Size: 1553
Fragments arranged by size:
1553 1367 942 926 901 423 262 197
27
SnaBI TAC'GTA
Cuts at: 493 4389 493
Size: 3896 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6598
SphI G_CATG'C
Cuts at: 1132 3956 1132
Size: 2824 3774
SspI AAT'ATT
Cuts at: 5 52 2824 4522 5
Size: 47 2772 1698 2081
Fragments arranged by size:
2772 2081 1698 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6598
StyI C'CwwG_G
Cuts at: 513 1065 2205 2314 3606 4037 513
Size: 552 1140 109 1292 431 3074
Fragments arranged by size:
3074 1292 1140 552 431 109
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2456 3499 3800 4625 4642 4795 4980
Size: 1255 876 1043 301 825 17 153 185
Cuts at: 4980 5425 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1043 876 825 445 301 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3114 3357 3378
Size: 274 80 33 51 1443 1137 243 21
Cuts at: 3378 3651 3693 4844 6556 96
Size: 273 42 1151 1712 138
Fragments arranged by size:
1712 1443 1151 1137 274 273 243 138
80 51 42 33 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3863 3895 6558 6590 3863
Size: 32 2663 32 3871
Fragments arranged by size:
3871 2663 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6598
XbaI T'CTAG_A
Cuts at: 2353 3934 2353
Size: 1581 5017
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6598
XmaI C'CCGG_G
Cuts at: 1870 2028 3923 1870
Size: 158 1895 4545
Fragments arranged by size:
4545 1895 158
XmnI GAAnn'nnTTC
Cuts at: 4727 4727
Size: 6598
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3589 4406 276
Size: 53 83 186 936 2055 817 2468
Fragments arranged by size:
2468 2055 936 817 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BbvCI BfrBI Bpu10I BsiWI
BstZ17I FseI FspAI NruI NsiI PacI PmlI PspXI
PsrI PvuII RsrII SacII SanDI SexAI SfiI SrfI
SwaI XcmI