Restriction map for
pFN21AA1590
(Circular) (Six-base) MAPSORT of: pFN21AA1590.seq Check: 5596 from: 1 to: 8881
pFN21AA1590 8881 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3022 3022
Size: 8881
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2661 6691 278
Size: 53 83 186 936 1125 4030 2468
Fragments arranged by size:
4030 2468 1125 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6224 1937
Size: 4287 4594
Acc65I G'GTAC_C
Cuts at: 6202 6202
Size: 8881
AclI AA'CG_TT
Cuts at: 1563 7008 7381 1563
Size: 5445 373 3063
Fragments arranged by size:
5445 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2377 2463 3212 3703 5378 5991 6026 6944
Size: 745 86 749 491 1675 613 35 918
Cuts at: 6944 8328 8596 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1675 1384 918 749 745 613 491
268 86 35
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8684
AflIII A'CryG_T
Cuts at: 1203 2473 7780 8757 1203
Size: 1270 5307 977 1327
Fragments arranged by size:
5307 1327 1270 977
AgeI A'CCGG_T
Cuts at: 7712 7712
Size: 8881
AhdI GACnn_n'nnGTC
Cuts at: 7610 7610
Size: 8881
AleI CACnn'nnGTG
Cuts at: 1166 4664 5759 1166
Size: 3498 1095 4288
Fragments arranged by size:
4288 3498 1095
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5127 1184
Size: 3943 4938
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2057 2089 4566 4598 2057
Size: 32 2477 32 6340
Fragments arranged by size:
6340 2477 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2645 4339 5224 5540 5746 6624 8196 2645
Size: 1694 885 316 206 878 1572 3330
Fragments arranged by size:
3330 1694 1572 885 878 316 206
ApaI G_GGCC'C
Cuts at: 5029 5029
Size: 8881
ApaLI G'TGCA_C
Cuts at: 5892 6938 8094 5892
Size: 1046 1156 6679
Fragments arranged by size:
6679 1156 1046
ApoI r'AATT_y
Cuts at: 1493 2347 2460 2708 3794 4037 4484 4791 4925
Size: 854 113 248 1086 243 447 307 134
Cuts at: 4925 5660 5930 6190 6416 6568 8677 1493
Size: 735 270 260 226 152 2109 1697
Fragments arranged by size:
2109 1697 1086 854 735 447 307 270
260 248 243 226 152 134 113
AseI AT'TA_AT
Cuts at: 160 1032 3096 7435 160
Size: 872 2064 4339 1606
Fragments arranged by size:
4339 2064 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8881
AvaI C'yCGr_G
Cuts at: 1870 1943 6206 1870
Size: 73 4263 4545
Fragments arranged by size:
4545 4263 73
BamHI G'GATC_C
Cuts at: 6211 6578 6211
Size: 367 8514
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5474 6202 7657 618
Size: 325 217 627 3687 728 1455 1842
Fragments arranged by size:
3687 1842 1455 728 627 325 217
BanII G_rGCy'C
Cuts at: 1391 5029 5151 6200 1391
Size: 3638 122 1049 4072
Fragments arranged by size:
4072 3638 1049 122
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 5847 5977 928
Size: 971 3948 130 3832
Fragments arranged by size:
3948 3832 971 130
BbvCI CC'TCA_GC
Cuts at: 4055 4055
Size: 8881
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2242 2276 7072 7106 1903
Size: 34 305 34 4796 34 3678
Fragments arranged by size:
4796 3678 305 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6773 7983 6773
Size: 1210 7671
BclI T'GATC_A
Cuts at: 1548 5907 1548
Size: 4359 4522
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7492 136
Size: 107 122 71 631 605 5820 1525
Fragments arranged by size:
5820 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2482 5581 8876 2482
Size: 3099 3295 2487
Fragments arranged by size:
3295 3099 2487
BlpI GC'TnA_GC
Cuts at: 5169 6298 5169
Size: 1129 7752
Bme1580I G_kGCm'C
Cuts at: 1790 5029 5084 5896 6942 8098 1790
Size: 3239 55 812 1046 1156 2573
Fragments arranged by size:
3239 2573 1156 1046 812 55
BmgBI CAC'GTC
Cuts at: 1318 4946 1318
Size: 3628 5253
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2148 2655 3559
Size: 416 53 83 399 24 731 507 904
Cuts at: 3559 6612 7570 442
Size: 3053 958 1753
Fragments arranged by size:
3053 1753 958 904 731 507 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 5160 1056
Size: 4104 4777
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4826 4826
Size: 8881
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2373 3914 3935 5039 5060 5135 5183 5204
Size: 1134 1541 21 1104 21 75 48 21
Cuts at: 5204 5558 7541 1239
Size: 354 1983 2579
Fragments arranged by size:
2579 1983 1541 1134 1104 354 75 48
21 21 21
Bpu10I CC'TnA_GC
Cuts at: 2869 3683 4055 6033 2869
Size: 814 372 1978 5717
Fragments arranged by size:
5717 1978 814 372
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3153 4656 6123 7006 7871 8169 3153
Size: 1503 1467 883 865 298 3865
Fragments arranged by size:
3865 1503 1467 883 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2676 3911 4097 7544 882
Size: 617 1177 1235 186 3447 2219
Fragments arranged by size:
3447 2219 1235 1177 617 186
BsaAI yAC'GTr
Cuts at: 493 1204 6672 493
Size: 711 5468 2702
Fragments arranged by size:
5468 2702 711
BsaBI GATnn'nnATC
Cuts at: 3377 5403 8875 3377
Size: 2026 3472 3383
Fragments arranged by size:
3472 3383 2026
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2658 5389 6688 7070
Size: 53 83 186 936 1125 2731 1299 382
Cuts at: 7070 7705 275
Size: 635 1451
Fragments arranged by size:
2731 1451 1299 1125 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2628 2789 7314 7712 7986 8133 8544 2628
Size: 161 4525 398 274 147 411 2965
Fragments arranged by size:
4525 2965 411 398 274 161 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2057 2087 4343 4373 4722
Size: 30 165 30 654 30 2256 30 349
Cuts at: 4722 4752 5634 5664 1178
Size: 30 882 30 4395
Fragments arranged by size:
4395 2256 882 654 349 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3983 4061 4274 4385 4466 5059 5874 1186
Size: 2797 78 213 111 81 593 815 4193
Fragments arranged by size:
4193 2797 815 593 213 111 81 78
BseYI C'CCAG_C
Cuts at: 3921 8084 8580 3921
Size: 4163 496 4222
Fragments arranged by size:
4222 4163 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2799 3965 1163
Size: 573 1063 1166 6079
Fragments arranged by size:
6079 1166 1063 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6331 7092 8120 1540
Size: 275 186 4330 761 1028 2301
Fragments arranged by size:
4330 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3572 5896 6039 6942 7027 8098 3572
Size: 2324 143 903 85 1071 4355
Fragments arranged by size:
4355 2324 1071 903 143 85
BsmI GAATG_Cn'
Cuts at: 4392 5862 6392 6485 4392
Size: 1470 530 93 6788
Fragments arranged by size:
6788 1470 530 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3444 3444
Size: 8881
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3572 5029 5084 5151 5896 6039 6200
Size: 399 1782 1457 55 67 745 143 161
Cuts at: 6200 6942 7027 8098 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1782 1457 1071 745 742 399 161
143 85 67 55
BspEI T'CCGG_A
Cuts at: 8544 8544
Size: 8881
BspHI T'CATG_A
Cuts at: 2994 6770 8500 2994
Size: 3776 1730 3375
Fragments arranged by size:
3776 3375 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2658 3022 4267 6236 844
Size: 1814 364 1245 1969 3489
Fragments arranged by size:
3489 1969 1814 1245 364
BsrBI CCG'CTC
Cuts at: 1394 1823 3898 5023 6768 1394
Size: 429 2075 1125 1745 3507
Fragments arranged by size:
3507 2075 1745 1125 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3508 4938 5823 7376 7550 65
Size: 1183 2260 1430 885 1553 174 1396
Fragments arranged by size:
2260 1553 1430 1396 1183 885 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7525 7712 1700
Size: 110 5715 187 2869
Fragments arranged by size:
5715 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8881
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8881
BssSI C'ACGA_G
Cuts at: 4286 4425 6941 7953 4286
Size: 139 2516 1012 5214
Fragments arranged by size:
5214 2516 1012 139
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4282 794
Size: 3488 5393
BstBI TT'CG_AA
Cuts at: 2437 4482 7685 2437
Size: 2045 3203 3633
Fragments arranged by size:
3633 3203 2045
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8881
BstYI r'GATC_y
Cuts at: 1972 2318 2376 2482 2745 4581 5581 6211 6578
Size: 346 58 106 263 1836 1000 630 367
Cuts at: 6578 6963 6980 8432 8518 8530 8876 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1836 1452 1000 630 385 367 346
346 263 106 86 58 17 12
Bsu36I CC'TnA_GG
Cuts at: 3323 6111 3323
Size: 2788 6093
BtgI C'CryG_G
Cuts at: 513 1065 2004 3462 513
Size: 552 939 1458 5932
Fragments arranged by size:
5932 1458 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8142
Fragments arranged by size:
8142 683 56
BtsI GCAGTG_nn'
Cuts at: 4929 6397 7191 7211 4929
Size: 1468 794 20 6599
Fragments arranged by size:
6599 1468 794 20
ClaI AT'CG_AT
Cuts at: 1554 3381 1554
Size: 1827 7054
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8846
DraI TTT'AAA
Cuts at: 3423 4596 6185 3423
Size: 1173 1589 6119
Fragments arranged by size:
6119 1589 1173
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2394 7888 809
Size: 507 1078 5494 1802
Fragments arranged by size:
5494 1802 1078 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2022 5031 5782
Size: 54 1425 50 275 18 192 3009 751
Cuts at: 5782 6328 7217 8846 8
Size: 546 889 1629 43
Fragments arranged by size:
3009 1629 1425 889 751 546 275 192
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6328 1537
Size: 275 4516 4090
Fragments arranged by size:
4516 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2146 2396 3054 3686 3692 4038 4082 4095
Size: 793 250 658 632 6 346 44 13
Cuts at: 4095 4296 4362 4370 5088 6811 1353
Size: 201 66 8 718 1723 3423
Fragments arranged by size:
3423 1723 793 718 658 632 346 250
201 66 44 13 8 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2509 7468 7842 7988 387
Size: 1064 138 920 4959 374 146 1280
Fragments arranged by size:
4959 1280 1064 920 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2373 2377 2463 3212 3703 3914 3935
Size: 393 741 4 86 749 491 211 21
Cuts at: 3935 5039 5060 5135 5183 5204 5378 5558 5991
Size: 1104 21 75 48 21 174 180 433
Cuts at: 5991 6026 6944 7541 8328 8596 1239
Size: 35 918 597 787 268 1524
Fragments arranged by size:
1524 1104 918 787 749 741 597 491
433 393 268 211 180 174 86 75
48 35 21 21 21 4
EcoNI CCTnn'n_nnAGG
Cuts at: 5186 5186
Size: 8881
EcoO109I rG'GnC_Cy
Cuts at: 2546 3561 4381 4528 4805 5026 5274 2546
Size: 1015 820 147 277 221 248 6153
Fragments arranged by size:
6153 1015 820 277 248 221 147
EcoRI G'AATT_C
Cuts at: 4484 4925 5930 6190 6568 4484
Size: 441 1005 260 378 6797
Fragments arranged by size:
6797 1005 441 378 260
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2767 2987 4886 2767
Size: 220 1899 6762
Fragments arranged by size:
6762 1899 220
FspI TGC'GCA
Cuts at: 7387 7387
Size: 8881
HaeII r_GCGC'y
Cuts at: 1678 5018 8028 1678
Size: 3340 3010 2531
Fragments arranged by size:
3340 3010 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2549 2581 3526 3558 3746
Size: 32 135 32 977 32 945 32 188
Cuts at: 3746 3778 4601 4633 7522 7554 7596 7628 1373
Size: 32 823 32 2889 32 42 32 2626
Fragments arranged by size:
2889 2626 977 945 823 188 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2949 5680 5970 6225 6471 669
Size: 988 281 1011 2731 290 255 246 3079
Fragments arranged by size:
3079 2731 1011 988 290 281 255 246
HindIII A'AGCT_T
Cuts at: 748 2427 4232 748
Size: 1679 1805 5397
Fragments arranged by size:
5397 1805 1679
HpaI GTT'AAC
Cuts at: 6471 6471
Size: 8881
KpnI G_GTAC'C
Cuts at: 6206 6206
Size: 8881
MfeI C'AATT_G
Cuts at: 6480 6480
Size: 8881
MluI A'CGCG_T
Cuts at: 8757 8757
Size: 8881
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2378 4156 4608 4746 7995 8179 1305
Size: 1073 1778 452 138 3249 184 2007
Fragments arranged by size:
3249 2007 1778 1073 452 184 138
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5033 5784 10
Size: 54 1425 343 3201 751 3107
Fragments arranged by size:
3201 3107 1425 751 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2339 4286 4664 5759 6839 7198 7357
Size: 648 1173 1947 378 1095 1080 359 159
Cuts at: 7357 518
Size: 2042
Fragments arranged by size:
2042 1947 1173 1095 1080 648 378 359
159
MspA1I CmG'CkG
Cuts at: 1606 4900 5224 5549 6297 6974 8122 8367 1606
Size: 3294 324 325 748 677 1148 245 2120
Fragments arranged by size:
3294 2120 1148 748 677 325 324 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 7390
Fragments arranged by size:
7390 939 552
NdeI CA'TA_TG
Cuts at: 387 2305 387
Size: 1918 6963
NheI G'CTAG_C
Cuts at: 1052 5156 1052
Size: 4104 4777
NotI GC'GGCC_GC
Cuts at: 6328 6328
Size: 8881
NspI r_CATG'y
Cuts at: 1132 1229 6239 7784 1132
Size: 97 5010 1545 2229
Fragments arranged by size:
5010 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 5077 1348
Size: 3729 5152
PciI A'CATG_T
Cuts at: 7780 7780
Size: 8881
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2418 3267 1108
Size: 1310 849 6722
Fragments arranged by size:
6722 1310 849
PmeI GTTT'AAAC
Cuts at: 6185 6185
Size: 8881
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2057 2089 6950 6982 8463 8495 2057
Size: 32 4861 32 1481 32 2443
Fragments arranged by size:
4861 2443 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3561 4381 4528 5274 3561
Size: 820 147 746 7168
Fragments arranged by size:
7168 820 746 147
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8881
PsiI TTA'TAA
Cuts at: 2286 2466 6451 2286
Size: 180 3985 4716
Fragments arranged by size:
4716 3985 180
PspOMI G'GGCC_C
Cuts at: 5025 5025
Size: 8881
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4713 4745 4713
Size: 32 8849
PstI C_TGCA'G
Cuts at: 830 3769 4083 4746 5064 5429 5440 6233 830
Size: 2939 314 663 318 365 11 793 3478
Fragments arranged by size:
3478 2939 793 663 365 318 314 11
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8881
PvuII CAG'CTG
Cuts at: 4900 5224 4900
Size: 324 8557
SalI G'TCGA_C
Cuts at: 1936 6223 1936
Size: 4287 4594
SanDI GG'GwC_CC
Cuts at: 3561 5274 3561
Size: 1713 7168
SapI GCTCTTCn'nnn_
Cuts at: 4296 5088 4296
Size: 792 8089
SbfI CC_TGCA'GG
Cuts at: 6233 6233
Size: 8881
ScaI AGT'ACT
Cuts at: 3046 3400 7129 8841 3046
Size: 354 3729 1712 3086
Fragments arranged by size:
3729 3086 1712 354
SexAI A'CCwGG_T
Cuts at: 4530 5331 4530
Size: 801 8080
SfcI C'TryA_G
Cuts at: 826 1045 3765 4079 4730 4742 5060 5425 5436
Size: 219 2720 314 651 12 318 365 11
Cuts at: 5436 6229 7364 8045 8236 826
Size: 793 1135 681 191 1471
Fragments arranged by size:
2720 1471 1135 793 681 651 365 318
314 219 191 12 11
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8881
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8881
SmaI CCC'GGG
Cuts at: 1872 6208 1872
Size: 4336 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3168 4671 6138 6985 7886 8148
Size: 197 926 1225 1503 1467 847 901 262
Cuts at: 8148 820
Size: 1553
Fragments arranged by size:
1553 1503 1467 1225 926 901 847 262
197
SnaBI TAC'GTA
Cuts at: 493 6672 493
Size: 6179 2702
SpeI A'CTAG_T
Cuts at: 152 5980 152
Size: 5828 3053
SphI G_CATG'C
Cuts at: 1132 6239 1132
Size: 5107 3774
SspI AAT'ATT
Cuts at: 5 52 2827 3298 6805 5
Size: 47 2775 471 3507 2081
Fragments arranged by size:
3507 2775 2081 471 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8881
StyI C'CwwG_G
Cuts at: 513 1065 2004 2971 3261 5673 6320 513
Size: 552 939 967 290 2412 647 3074
Fragments arranged by size:
3074 2412 967 939 647 552 290
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2002 3749 4351 5762 5834 6908 6925
Size: 1255 422 1747 602 1411 72 1074 17
Cuts at: 6925 7078 7263 7708 325
Size: 153 185 445 1498
Fragments arranged by size:
1747 1498 1411 1255 1074 602 445 422
185 153 72 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2578 3044 3398
Size: 274 80 33 51 1443 601 466 354
Cuts at: 3398 4701 5429 7127 8839 96
Size: 1303 728 1698 1712 138
Fragments arranged by size:
1712 1698 1443 1303 728 601 466 354
274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4343 4375 5634 5666 8841 8873 4343
Size: 32 1259 32 3175 32 4351
Fragments arranged by size:
4351 3175 1259 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8881
XbaI T'CTAG_A
Cuts at: 4619 6217 4619
Size: 1598 7283
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4237 4237
Size: 8881
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8881
XmaI C'CCGG_G
Cuts at: 1870 6206 1870
Size: 4336 4545
XmnI GAAnn'nnTTC
Cuts at: 3225 4392 7010 3225
Size: 1167 2618 5096
Fragments arranged by size:
5096 2618 1167
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2659 6689 276
Size: 53 83 186 936 1125 4030 2468
Fragments arranged by size:
4030 2468 1125 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI
BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI
RsrII SacI SacII SfiI SfoI SrfI SwaI