Restriction map for
pFN21AA1588
(Circular) (Six-base) MAPSORT of: pFN21AA1588.seq Check: 182 from: 1 to: 6490
pFN21AA1588 6490 bp
With 182 enzymes: SgfI *
August 16, 2010 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4300 278
Size: 53 83 186 936 2764 2468
Fragments arranged by size:
2764 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3833 1937
Size: 1896 4594
Acc65I G'GTAC_C
Cuts at: 3811 3811
Size: 6490
AclI AA'CG_TT
Cuts at: 1563 4617 4990 1563
Size: 3054 373 3063
Fragments arranged by size:
3063 3054 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2775 3520 3575 3604 3688 4553 5937 6205
Size: 1143 745 55 29 84 865 1384 268
Cuts at: 6205 1632
Size: 1917
Fragments arranged by size:
1917 1384 1143 865 745 268 84 55
29
AflII C'TTAA_G
Cuts at: 820 1017 3302 820
Size: 197 2285 4008
Fragments arranged by size:
4008 2285 197
AflIII A'CryG_T
Cuts at: 1203 3090 5389 6366 1203
Size: 1887 2299 977 1327
Fragments arranged by size:
2299 1887 1327 977
AgeI A'CCGG_T
Cuts at: 5321 5321
Size: 6490
AhdI GACnn_n'nnGTC
Cuts at: 5219 5219
Size: 6490
AleI CACnn'nnGTG
Cuts at: 1166 3018 1166
Size: 1852 4638
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6490
AlwNI CAG_nnn'CTG
Cuts at: 2766 4233 5805 2766
Size: 1467 1572 3451
Fragments arranged by size:
3451 1572 1467
ApaLI G'TGCA_C
Cuts at: 3094 3644 4547 5703 3094
Size: 550 903 1156 3881
Fragments arranged by size:
3881 1156 903 550
ApoI r'AATT_y
Cuts at: 1493 2069 2107 3799 4025 4177 6286 1493
Size: 576 38 1692 226 152 2109 1697
Fragments arranged by size:
2109 1697 1692 576 226 152 38
AseI AT'TA_AT
Cuts at: 160 1032 5044 160
Size: 872 4012 1606
Fragments arranged by size:
4012 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6490
AvaI C'yCGr_G
Cuts at: 1870 1943 3815 1870
Size: 73 1872 4545
Fragments arranged by size:
4545 1872 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2279 2312 2279
Size: 33 6457
BamHI G'GATC_C
Cuts at: 3654 3820 4187 3654
Size: 166 367 5957
Fragments arranged by size:
5957 367 166
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3811 5266 618
Size: 325 217 627 2024 1455 1842
Fragments arranged by size:
2024 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3809 1391
Size: 2418 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3173 3284 3442 3563 928
Size: 971 1274 111 158 121 3855
Fragments arranged by size:
3855 1274 971 158 121 111
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4681 4715 1903
Size: 34 2744 34 3678
Fragments arranged by size:
3678 2744 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4382 5592 4382
Size: 1210 5280
BclI T'GATC_A
Cuts at: 1548 2929 1548
Size: 1381 5109
BfrBI ATG'CAT
Cuts at: 2668 2861 2668
Size: 193 6297
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5101 136
Size: 107 122 71 631 605 3429 1525
Fragments arranged by size:
3429 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6485 6485
Size: 6490
BlpI GC'TnA_GC
Cuts at: 3907 3907
Size: 6490
Bme1580I G_kGCm'C
Cuts at: 1790 3098 3648 4551 5707 1790
Size: 1308 550 903 1156 2573
Fragments arranged by size:
2573 1308 1156 903 550
BmgBI CAC'GTC
Cuts at: 1318 2034 1318
Size: 716 5774
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2145 2300 2451
Size: 416 53 83 399 24 728 155 151
Cuts at: 2451 4221 5179 442
Size: 1770 958 1753
Fragments arranged by size:
1770 1753 958 728 416 399 155 151
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6490
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2385 3773 2385
Size: 1388 5102
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2042 2156 2285 3348 3770 5150 1239
Size: 803 114 129 1063 422 1380 2579
Fragments arranged by size:
2579 1380 1063 803 422 129 114
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4615 5480 5778 4615
Size: 865 298 5327
Fragments arranged by size:
5327 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5153 882
Size: 617 3654 2219
Fragments arranged by size:
3654 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3061 3733 4281 493
Size: 711 1857 672 548 2702
Fragments arranged by size:
2702 1857 711 672 548
BsaBI GATnn'nnATC
Cuts at: 3239 6484 3239
Size: 3245 3245
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4297 4679 5314 275
Size: 53 83 186 936 2764 382 635 1451
Fragments arranged by size:
2764 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2865 2990 4923 5321 5595 5742 6153 2865
Size: 125 1933 398 274 147 411 3202
Fragments arranged by size:
3202 1933 411 398 274 147 125
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3575 3605 1178
Size: 30 165 30 2172 30 4063
Fragments arranged by size:
4063 2172 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2354 2357 2372 1186
Size: 1168 3 15 5304
Fragments arranged by size:
5304 1168 15 3
BseYI C'CCAG_C
Cuts at: 2312 5693 6189 2312
Size: 3381 496 2613
Fragments arranged by size:
3381 2613 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2146 2406 2779 1163
Size: 573 410 260 373 4874
Fragments arranged by size:
4874 573 410 373 260
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2953 3940 4701 5729 1540
Size: 275 186 952 987 761 1028 2301
Fragments arranged by size:
2301 1028 987 952 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3060 3098 3648 4551 4636 5707 3060
Size: 38 550 903 85 1071 3843
Fragments arranged by size:
3843 1071 903 550 85 38
BsiWI C'GTAC_G
Cuts at: 3591 3591
Size: 6490
BsmI GAATG_Cn'
Cuts at: 2861 3191 3434 3602 3686 4001 4094 2861
Size: 330 243 168 84 315 93 5257
Fragments arranged by size:
5257 330 315 243 168 93 84
BsmBI CGTCTCn'nnnn_
Cuts at: 3412 3412
Size: 6490
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3060 3098 3648 3809 4551 4636 5707
Size: 399 1270 38 550 161 742 85 1071
Cuts at: 5707 1391
Size: 2174
Fragments arranged by size:
2174 1270 1071 742 550 399 161 85
38
BspEI T'CCGG_A
Cuts at: 2865 6153 2865
Size: 3288 3202
BspHI T'CATG_A
Cuts at: 4379 6109 4379
Size: 1730 4760
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3845 844
Size: 3001 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4377 1394
Size: 429 2554 3507
Fragments arranged by size:
3507 2554 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4985 5159 65
Size: 1183 3737 174 1396
Fragments arranged by size:
3737 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2480 5134 5321 1700
Size: 110 670 2654 187 2869
Fragments arranged by size:
2869 2654 670 187 110
BsrGI T'GTAC_A
Cuts at: 96 2247 96
Size: 2151 4339
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6490
BssSI C'ACGA_G
Cuts at: 2701 3209 4550 5562 2701
Size: 508 1341 1012 3629
Fragments arranged by size:
3629 1341 1012 508
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2766 3102 794
Size: 1972 336 4182
Fragments arranged by size:
4182 1972 336
BstBI TT'CG_AA
Cuts at: 2520 5294 2520
Size: 2774 3716
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6490
BstYI r'GATC_y
Cuts at: 1972 3654 3820 4187 4572 4589 6041 6127 6139
Size: 1682 166 367 385 17 1452 86 12
Cuts at: 6139 6485 1972
Size: 346 1977
Fragments arranged by size:
1977 1682 1452 385 367 346 166 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2798 3722 2798
Size: 924 5566
BtgI C'CryG_G
Cuts at: 513 1065 2004 3255 513
Size: 552 939 1251 3748
Fragments arranged by size:
3748 1251 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2953 487
Size: 683 56 1727 4024
Fragments arranged by size:
4024 1727 683 56
BtsI GCAGTG_nn'
Cuts at: 2134 3107 3275 3527 4006 4800 4820 2134
Size: 973 168 252 479 794 20 3804
Fragments arranged by size:
3804 973 794 479 252 168 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6490
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6455
DraI TTT'AAA
Cuts at: 3794 3794
Size: 6490
DraIII CAC_nnn'GTG
Cuts at: 2153 3102 3160 2153
Size: 949 58 5483
Fragments arranged by size:
5483 949 58
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5497 809
Size: 507 4181 1802
Fragments arranged by size:
4181 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2950 3937 4826
Size: 54 1425 50 275 18 1120 987 889
Cuts at: 4826 6455 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1120 987 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2950 3937 1537
Size: 275 1138 987 4090
Fragments arranged by size:
4090 1138 987 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4420 1353
Size: 3067 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3487 3667 5077 5451 5597 387
Size: 1064 138 1898 180 1410 374 146 1280
Fragments arranged by size:
1898 1410 1280 1064 374 180 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2042 2156 2285 2775 3348 3520 3575
Size: 393 410 114 129 490 573 172 55
Cuts at: 3575 3604 3688 3770 4553 5150 5937 6205 1239
Size: 29 84 82 783 597 787 268 1524
Fragments arranged by size:
1524 787 783 597 573 490 410 393
268 172 129 114 84 82 55 29
EcoO109I rG'GnC_Cy
Cuts at: 2580 3000 2580
Size: 420 6070
EcoRI G'AATT_C
Cuts at: 3799 4177 3799
Size: 378 6112
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3148 3148
Size: 6490
FspI TGC'GCA
Cuts at: 4996 4996
Size: 6490
HaeII r_GCGC'y
Cuts at: 1678 2370 2382 5637 1678
Size: 692 12 3255 2531
Fragments arranged by size:
3255 2531 692 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2387 2419 2569 2601 3197
Size: 32 135 32 815 32 150 32 596
Cuts at: 3197 3229 3449 3481 3701 3733 5131 5163 5205
Size: 32 220 32 220 32 1398 32 42
Cuts at: 5205 5237 1373
Size: 32 2626
Fragments arranged by size:
2626 1398 815 596 220 220 150 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3549 3834 4080 669
Size: 988 281 1611 285 246 3079
Fragments arranged by size:
3079 1611 988 285 281 246
HindIII A'AGCT_T
Cuts at: 748 3027 3363 748
Size: 2279 336 3875
Fragments arranged by size:
3875 2279 336
HpaI GTT'AAC
Cuts at: 4080 4080
Size: 6490
KpnI G_GTAC'C
Cuts at: 3815 3815
Size: 6490
MfeI C'AATT_G
Cuts at: 4089 4089
Size: 6490
MluI A'CGCG_T
Cuts at: 3090 6366 3090
Size: 3276 3214
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3153 3657 5604 5788 1305
Size: 1848 504 1947 184 2007
Fragments arranged by size:
2007 1947 1848 504 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4668
Fragments arranged by size:
4668 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2645 3018 3343 3772 4448 4807 4966
Size: 648 1479 373 325 429 676 359 159
Cuts at: 4966 518
Size: 2042
Fragments arranged by size:
2042 1479 676 648 429 373 359 325
159
MspA1I CmG'CkG
Cuts at: 1606 3120 3906 4583 5731 5976 1606
Size: 1514 786 677 1148 245 2120
Fragments arranged by size:
2120 1514 1148 786 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4999
Fragments arranged by size:
4999 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6490
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6490
NotI GC'GGCC_GC
Cuts at: 3937 3937
Size: 6490
NsiI A_TGCA'T
Cuts at: 2670 2863 2670
Size: 193 6297
NspI r_CATG'y
Cuts at: 1132 1229 2650 2896 3568 3848 5393 1132
Size: 97 1421 246 672 280 1545 2229
Fragments arranged by size:
2229 1545 1421 672 280 246 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6490
PciI A'CATG_T
Cuts at: 5389 5389
Size: 6490
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2110 3160 1108
Size: 1002 1050 4438
Fragments arranged by size:
4438 1050 1002
PfoI T'CCnGG_A
Cuts at: 2058 3215 2058
Size: 1157 5333
PmeI GTTT'AAAC
Cuts at: 3794 3794
Size: 6490
PmlI CAC'GTG
Cuts at: 3061 3733 3061
Size: 672 5818
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4559 4591 6072 6104 4559
Size: 32 1481 32 4945
Fragments arranged by size:
4945 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2844 1613
Size: 1231 5259
PsiI TTA'TAA
Cuts at: 4060 4060
Size: 6490
PstI C_TGCA'G
Cuts at: 830 2846 3842 830
Size: 2016 996 3478
Fragments arranged by size:
3478 2016 996
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6490
SalI G'TCGA_C
Cuts at: 1936 3832 1936
Size: 1896 4594
SbfI CC_TGCA'GG
Cuts at: 3842 3842
Size: 6490
ScaI AGT'ACT
Cuts at: 4738 6450 4738
Size: 1712 4778
SfcI C'TryA_G
Cuts at: 826 1045 2272 2842 3838 4973 5654 5845 826
Size: 219 1227 570 996 1135 681 191 1471
Fragments arranged by size:
1471 1227 1135 996 681 570 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6490
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6490
SmaI CCC'GGG
Cuts at: 1872 3817 1872
Size: 1945 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3302 4594 5495 5757 820
Size: 197 926 1359 1292 901 262 1553
Fragments arranged by size:
1553 1359 1292 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4281 493
Size: 3788 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6490
SphI G_CATG'C
Cuts at: 1132 3848 1132
Size: 2716 3774
SspI AAT'ATT
Cuts at: 5 52 4414 5
Size: 47 4362 2081
Fragments arranged by size:
4362 2081 47
StuI AGG'CCT
Cuts at: 1517 2917 3533 3617 1517
Size: 1400 616 84 4390
Fragments arranged by size:
4390 1400 616 84
StyI C'CwwG_G
Cuts at: 513 1065 2004 3929 513
Size: 552 939 1925 3074
Fragments arranged by size:
3074 1925 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2054 2164 4517 4534 4687 4872 5317
Size: 1255 474 110 2353 17 153 185 445
Cuts at: 5317 325
Size: 1498
Fragments arranged by size:
2353 1498 1255 474 445 185 153 110
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2247 4736 6448
Size: 274 80 33 51 1443 270 2489 1712
Cuts at: 6448 96
Size: 138
Fragments arranged by size:
2489 1712 1443 274 270 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6450 6482 6450
Size: 32 6458
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6490
XbaI T'CTAG_A
Cuts at: 3826 3826
Size: 6490
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6490
XmaI C'CCGG_G
Cuts at: 1870 3815 1870
Size: 1945 4545
XmnI GAAnn'nnTTC
Cuts at: 2777 3029 3281 3365 3449 3617 3701 4619 2777
Size: 252 252 84 84 168 84 918 4648
Fragments arranged by size:
4648 918 252 252 168 84 84 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4298 276
Size: 53 83 186 936 2764 2468
Fragments arranged by size:
2764 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
Bpu10I BstXI BstZ17I EcoICRI EcoNI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI