Restriction map for
pFN21AA1584
(Circular) (Six-base) MAPSORT of: pFN21AA1584.seq Check: 4353 from: 1 to: 7603
pFN21AA1584 7603 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3050 3050
Size: 7603
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5413 278
Size: 53 83 186 936 3877 2468
Fragments arranged by size:
3877 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4875 4946 1937
Size: 2938 71 4594
Fragments arranged by size:
4594 2938 71
Acc65I G'GTAC_C
Cuts at: 4924 4924
Size: 7603
AclI AA'CG_TT
Cuts at: 1563 5730 6103 1563
Size: 4167 373 3063
Fragments arranged by size:
4167 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2292 2529 2571 2586 2920 3510 3575 4754
Size: 660 237 42 15 334 590 65 1179
Cuts at: 4754 4776 5666 7050 7318 1632
Size: 22 890 1384 268 1917
Fragments arranged by size:
1917 1384 1179 890 660 590 334 268
237 65 42 22 15
AflII C'TTAA_G
Cuts at: 820 1017 4096 820
Size: 197 3079 4327
Fragments arranged by size:
4327 3079 197
AflIII A'CryG_T
Cuts at: 1203 3166 4495 6502 7479 1203
Size: 1963 1329 2007 977 1327
Fragments arranged by size:
2007 1963 1329 1327 977
AgeI A'CCGG_T
Cuts at: 6434 6434
Size: 7603
AhdI GACnn_n'nnGTC
Cuts at: 6332 6332
Size: 7603
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7603
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7603
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2662 2694 2662
Size: 32 7571
AlwNI CAG_nnn'CTG
Cuts at: 2270 5346 6918 2270
Size: 3076 1572 2955
Fragments arranged by size:
3076 2955 1572
ApaLI G'TGCA_C
Cuts at: 2193 5660 6816 2193
Size: 3467 1156 2980
Fragments arranged by size:
3467 2980 1156
ApoI r'AATT_y
Cuts at: 1493 2284 2566 2795 2949 3119 3811 4270 4330
Size: 791 282 229 154 170 692 459 60
Cuts at: 4330 4912 5138 5290 7399 1493
Size: 582 226 152 2109 1697
Fragments arranged by size:
2109 1697 791 692 582 459 282 229
226 170 154 152 60
AseI AT'TA_AT
Cuts at: 160 1032 6157 160
Size: 872 5125 1606
Fragments arranged by size:
5125 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7603
AvaI C'yCGr_G
Cuts at: 1870 1943 4928 1870
Size: 73 2985 4545
Fragments arranged by size:
4545 2985 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4733 4766 4733
Size: 33 7570
BamHI G'GATC_C
Cuts at: 2722 4933 5300 2722
Size: 2211 367 5025
Fragments arranged by size:
5025 2211 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4924 6379 618
Size: 325 217 627 3137 1455 1842
Fragments arranged by size:
3137 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4922 1391
Size: 3531 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2918 928
Size: 971 1019 5613
Fragments arranged by size:
5613 1019 971
BbvCI CC'TCA_GC
Cuts at: 2572 2572
Size: 7603
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5794 5828 1903
Size: 34 3857 34 3678
Fragments arranged by size:
3857 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2410 4163 5495 6705 2410
Size: 1753 1332 1210 3308
Fragments arranged by size:
3308 1753 1332 1210
BclI T'GATC_A
Cuts at: 1548 2850 4555 4645 4819 1548
Size: 1302 1705 90 174 4332
Fragments arranged by size:
4332 1705 1302 174 90
BfrBI ATG'CAT
Cuts at: 3355 3843 3355
Size: 488 7115
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6214 136
Size: 107 122 71 631 605 4542 1525
Fragments arranged by size:
4542 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2303 7598 2303
Size: 5295 2308
BlpI GC'TnA_GC
Cuts at: 5020 5020
Size: 7603
Bme1580I G_kGCm'C
Cuts at: 1790 2197 5664 6820 1790
Size: 407 3467 1156 2573
Fragments arranged by size:
3467 2573 1156 407
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7603
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5334 6292 442
Size: 416 53 83 399 24 3917 958 1753
Fragments arranged by size:
3917 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2630 1056
Size: 1574 6029
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2580 4561 4860 6263 1239
Size: 1341 1981 299 1403 2579
Fragments arranged by size:
2579 1981 1403 1341 299
Bpu10I CC'TnA_GC
Cuts at: 2572 2572
Size: 7603
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3023 4766 5728 6593 6891 3023
Size: 1743 962 865 298 3735
Fragments arranged by size:
3735 1743 962 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6266 882
Size: 617 4767 2219
Fragments arranged by size:
4767 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3891 5394 493
Size: 711 2687 1503 2702
Fragments arranged by size:
2702 2687 1503 711
BsaBI GATnn'nnATC
Cuts at: 3247 4624 7597 3247
Size: 1377 2973 3253
Fragments arranged by size:
3253 2973 1377
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5410 5792 6427 275
Size: 53 83 186 936 3877 382 635 1451
Fragments arranged by size:
3877 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6036 6434 6708 6855 7266 6036
Size: 398 274 147 411 6373
Fragments arranged by size:
6373 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7378
Fragments arranged by size:
7378 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2051 1186
Size: 865 6738
BseYI C'CCAG_C
Cuts at: 2162 2543 3024 6806 7302 2162
Size: 381 481 3782 496 2463
Fragments arranged by size:
3782 2463 496 481 381
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4482 1163
Size: 573 2746 4284
Fragments arranged by size:
4284 2746 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5053 5814 6842 1540
Size: 275 186 3052 761 1028 2301
Fragments arranged by size:
3052 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2197 4170 5664 5749 6820 2197
Size: 1973 1494 85 1071 2980
Fragments arranged by size:
2980 1973 1494 1071 85
BsiWI C'GTAC_G
Cuts at: 3891 3891
Size: 7603
BsmI GAATG_Cn'
Cuts at: 2219 3831 4006 5114 5207 2219
Size: 1612 175 1108 93 4615
Fragments arranged by size:
4615 1612 1108 175 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2197 4170 4922 5664 5749 6820 1391
Size: 399 407 1973 752 742 85 1071 2174
Fragments arranged by size:
2174 1973 1071 752 742 407 399 85
BspEI T'CCGG_A
Cuts at: 7266 7266
Size: 7603
BspHI T'CATG_A
Cuts at: 5492 7222 5492
Size: 1730 5873
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2453 3050 4958 844
Size: 1609 597 1908 3489
Fragments arranged by size:
3489 1908 1609 597
BsrBI CCG'CTC
Cuts at: 1394 1823 5490 1394
Size: 429 3667 3507
Fragments arranged by size:
3667 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6098 6272 65
Size: 1183 4850 174 1396
Fragments arranged by size:
4850 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6247 6434 1700
Size: 110 4437 187 2869
Fragments arranged by size:
4437 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3395 96
Size: 3299 4304
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7603
BssSI C'ACGA_G
Cuts at: 2367 5663 6675 2367
Size: 3296 1012 3295
Fragments arranged by size:
3296 3295 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7603
BstBI TT'CG_AA
Cuts at: 6407 6407
Size: 7603
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7603
BstXI CCAn_nnnn'nTGG
Cuts at: 4421 4421
Size: 7603
BstYI r'GATC_y
Cuts at: 1972 2303 2722 4933 5300 5685 5702 7154 7240
Size: 331 419 2211 367 385 17 1452 86
Cuts at: 7240 7252 7598 1972
Size: 12 346 1977
Fragments arranged by size:
2211 1977 1452 419 385 367 346 331
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2048 513
Size: 552 939 44 6068
Fragments arranged by size:
6068 939 552 44
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6864
Fragments arranged by size:
6864 683 56
BtsI GCAGTG_nn'
Cuts at: 3022 3049 3092 5119 5913 5933 3022
Size: 27 43 2027 794 20 4692
Fragments arranged by size:
4692 2027 794 43 27 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7603
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7568
DraI TTT'AAA
Cuts at: 2929 4907 2929
Size: 1978 5625
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6610 809
Size: 507 5294 1802
Fragments arranged by size:
5294 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5050 5939 7568
Size: 54 1425 50 275 18 3220 889 1629
Cuts at: 7568 8
Size: 43
Fragments arranged by size:
3220 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5050 1537
Size: 275 3238 4090
Fragments arranged by size:
4090 3238 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2028 5533 1353
Size: 675 3505 3423
Fragments arranged by size:
3505 3423 675
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6190 6564 6710 387
Size: 1064 138 4601 374 146 1280
Fragments arranged by size:
4601 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2292 2529 2571 2580 2586 2920 3510
Size: 393 660 237 42 9 6 334 590
Cuts at: 3510 3575 4561 4754 4776 4860 5666 6263 7050
Size: 65 986 193 22 84 806 597 787
Cuts at: 7050 7318 1239
Size: 268 1524
Fragments arranged by size:
1524 986 806 787 660 597 590 393
334 268 237 193 84 65 42 22
9 6
EcoRI G'AATT_C
Cuts at: 4912 5290 4912
Size: 378 7225
EcoRV GAT'ATC
Cuts at: 2115 2115
Size: 7603
FspI TGC'GCA
Cuts at: 6109 6109
Size: 7603
HaeII r_GCGC'y
Cuts at: 1678 6750 1678
Size: 5072 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2288 2320 4552 4559 4584
Size: 32 135 32 716 32 2232 7 25
Cuts at: 4584 4591 6244 6276 6318 6350 1373
Size: 7 1653 32 42 32 2626
Fragments arranged by size:
2626 2232 1653 716 135 42 32 32
32 32 32 25 7 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2158 2237 4103 4947 5193 669
Size: 988 281 220 79 1866 844 246 3079
Fragments arranged by size:
3079 1866 988 844 281 246 220 79
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7603
HpaI GTT'AAC
Cuts at: 2158 5193 2158
Size: 3035 4568
KpnI G_GTAC'C
Cuts at: 4928 4928
Size: 7603
MfeI C'AATT_G
Cuts at: 5202 5202
Size: 7603
MluI A'CGCG_T
Cuts at: 7479 7479
Size: 7603
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2276 3511 3699 6717 6901 1305
Size: 971 1235 188 3018 184 2007
Fragments arranged by size:
3018 2007 1235 971 188 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5781
Fragments arranged by size:
5781 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5561 5920 6079 518
Size: 648 4395 359 159 2042
Fragments arranged by size:
4395 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2576 5019 5696 6844 7089 1606
Size: 970 2443 677 1148 245 2120
Fragments arranged by size:
2443 2120 1148 970 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2048 513
Size: 552 939 44 6068
Fragments arranged by size:
6068 939 552 44
NdeI CA'TA_TG
Cuts at: 387 3183 3217 3951 387
Size: 2796 34 734 4039
Fragments arranged by size:
4039 2796 734 34
NheI G'CTAG_C
Cuts at: 1052 2626 1052
Size: 1574 6029
NotI GC'GGCC_GC
Cuts at: 5050 5050
Size: 7603
NsiI A_TGCA'T
Cuts at: 3357 3845 3357
Size: 488 7115
NspI r_CATG'y
Cuts at: 1132 1229 3170 3426 4961 6506 1132
Size: 97 1941 256 1535 1545 2229
Fragments arranged by size:
2229 1941 1545 1535 256 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7603
PciI A'CATG_T
Cuts at: 3166 6502 3166
Size: 3336 4267
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7603
PfoI T'CCnGG_A
Cuts at: 2898 3499 4837 2898
Size: 601 1338 5664
Fragments arranged by size:
5664 1338 601
PmeI GTTT'AAAC
Cuts at: 4907 4907
Size: 7603
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2461 2493 5672 5704 7185 7217 2461
Size: 32 3179 32 1481 32 2847
Fragments arranged by size:
3179 2847 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7603
PsiI TTA'TAA
Cuts at: 3470 5173 3470
Size: 1703 5900
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3496 3528 3496
Size: 32 7571
PstI C_TGCA'G
Cuts at: 830 3414 4955 830
Size: 2584 1541 3478
Fragments arranged by size:
3478 2584 1541
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7603
PvuII CAG'CTG
Cuts at: 2576 2576
Size: 7603
SalI G'TCGA_C
Cuts at: 1936 4945 1936
Size: 3009 4594
SbfI CC_TGCA'GG
Cuts at: 4955 4955
Size: 7603
ScaI AGT'ACT
Cuts at: 5851 7563 5851
Size: 1712 5891
SexAI A'CCwGG_T
Cuts at: 2344 3202 2344
Size: 858 6745
SfcI C'TryA_G
Cuts at: 826 1045 3410 3901 3928 4951 6086 6767 6958
Size: 219 2365 491 27 1023 1135 681 191
Cuts at: 6958 826
Size: 1471
Fragments arranged by size:
2365 1471 1135 1023 681 491 219 191
27
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7603
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7603
SmaI CCC'GGG
Cuts at: 1872 4930 1872
Size: 3058 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3002 4096 4745 5707 6608 6870
Size: 197 926 1059 1094 649 962 901 262
Cuts at: 6870 820
Size: 1553
Fragments arranged by size:
1553 1094 1059 962 926 901 649 262
197
SnaBI TAC'GTA
Cuts at: 493 3891 5394 493
Size: 3398 1503 2702
Fragments arranged by size:
3398 2702 1503
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7603
SphI G_CATG'C
Cuts at: 1132 4961 1132
Size: 3829 3774
SspI AAT'ATT
Cuts at: 5 52 2143 4456 4606 5527 5
Size: 47 2091 2313 150 921 2081
Fragments arranged by size:
2313 2091 2081 921 150 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7603
StyI C'CwwG_G
Cuts at: 513 1065 2004 2048 2437 3605 5042 513
Size: 552 939 44 389 1168 1437 3074
Fragments arranged by size:
3074 1437 1168 939 552 389 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5630 5647 5800 5985 6430 325
Size: 1255 4050 17 153 185 445 1498
Fragments arranged by size:
4050 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2804 3101 3395
Size: 274 80 33 51 1443 827 297 294
Cuts at: 3395 5849 7561 96
Size: 2454 1712 138
Fragments arranged by size:
2454 1712 1443 827 297 294 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7563 7595 7563
Size: 32 7571
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7603
XbaI T'CTAG_A
Cuts at: 2300 4939 2300
Size: 2639 4964
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7603
XmaI C'CCGG_G
Cuts at: 1870 4928 1870
Size: 3058 4545
XmnI GAAnn'nnTTC
Cuts at: 3168 5732 3168
Size: 2564 5039
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5411 276
Size: 53 83 186 936 3877 2468
Fragments arranged by size:
3877 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BbeI BplI BsmBI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI
PspOMI PspXI RsrII SacI SacII SanDI SapI SfiI
SfoI SrfI SwaI XcmI