Restriction map for
pFN21AA1579
(Circular) (Six-base) MAPSORT of: pFN21AA1579.seq Check: 7736 from: 1 to: 6739
pFN21AA1579 6739 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4549 278
Size: 53 83 186 936 3013 2468
Fragments arranged by size:
3013 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2620 4035 4082 1937
Size: 683 1415 47 4594
Fragments arranged by size:
4594 1415 683 47
Acc65I G'GTAC_C
Cuts at: 4060 4060
Size: 6739
AclI AA'CG_TT
Cuts at: 1563 4866 5239 1563
Size: 3303 373 3063
Fragments arranged by size:
3303 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2399 2661 2880 3284 3536 4802 6186 6454
Size: 767 262 219 404 252 1266 1384 268
Cuts at: 6454 1632
Size: 1917
Fragments arranged by size:
1917 1384 1266 767 404 268 262 252
219
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6542
AflIII A'CryG_T
Cuts at: 1203 2977 5638 6615 1203
Size: 1774 2661 977 1327
Fragments arranged by size:
2661 1774 1327 977
AgeI A'CCGG_T
Cuts at: 5570 5570
Size: 6739
AhdI GACnn_n'nnGTC
Cuts at: 5468 5468
Size: 6739
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6739
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6739
AlwNI CAG_nnn'CTG
Cuts at: 2247 3647 4482 6054 2247
Size: 1400 835 1572 2932
Fragments arranged by size:
2932 1572 1400 835
ApaI G_GGCC'C
Cuts at: 2102 2102
Size: 6739
ApaLI G'TGCA_C
Cuts at: 4796 5952 4796
Size: 1156 5583
ApoI r'AATT_y
Cuts at: 1493 2754 3526 3693 3721 4048 4274 4426 6535
Size: 1261 772 167 28 327 226 152 2109
Cuts at: 6535 1493
Size: 1697
Fragments arranged by size:
2109 1697 1261 772 327 226 167 152
28
AseI AT'TA_AT
Cuts at: 160 1032 3230 5293 160
Size: 872 2198 2063 1606
Fragments arranged by size:
2198 2063 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6739
AvaI C'yCGr_G
Cuts at: 1870 1943 4064 1870
Size: 73 2121 4545
Fragments arranged by size:
4545 2121 73
BamHI G'GATC_C
Cuts at: 4069 4436 4069
Size: 367 6372
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2123 4060 5515 618
Size: 325 217 627 336 1937 1455 1842
Fragments arranged by size:
1937 1842 1455 627 336 325 217
BanII G_rGCy'C
Cuts at: 1391 2102 2685 3285 4058 1391
Size: 711 583 600 773 4072
Fragments arranged by size:
4072 773 711 600 583
BbeI G_GCGC'C
Cuts at: 2127 2127
Size: 6739
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5768
BbvCI CC'TCA_GC
Cuts at: 2104 2104
Size: 6739
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4930 4964 1903
Size: 34 2993 34 3678
Fragments arranged by size:
3678 2993 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4631 5841 4631
Size: 1210 5529
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6739
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 5350 136
Size: 107 122 71 631 605 337 3341 1525
Fragments arranged by size:
3341 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 6734 6734
Size: 6739
BlpI GC'TnA_GC
Cuts at: 2198 4156 2198
Size: 1958 4781
Bme1580I G_kGCm'C
Cuts at: 1790 2102 2454 3477 3992 4800 5956 1790
Size: 312 352 1023 515 808 1156 2573
Fragments arranged by size:
2573 1156 1023 808 515 352 312
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6739
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2715 3076 3735
Size: 416 53 83 399 24 1298 361 659
Cuts at: 3735 4470 5428 442
Size: 735 958 1753
Fragments arranged by size:
1753 1298 958 735 659 416 399 361
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6739
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2627 2952 5399 1239
Size: 1388 325 2447 2579
Fragments arranged by size:
2579 2447 1388 325
Bpu10I CC'TnA_GC
Cuts at: 2104 2104
Size: 6739
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4864 5729 6027 4864
Size: 865 298 5576
Fragments arranged by size:
5576 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5402 882
Size: 617 3903 2219
Fragments arranged by size:
3903 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4530 493
Size: 711 3326 2702
Fragments arranged by size:
3326 2702 711
BsaBI GATnn'nnATC
Cuts at: 2664 6733 2664
Size: 4069 2670
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2124 4546 4928 5563
Size: 53 83 186 936 591 2422 382 635
Cuts at: 5563 275
Size: 1451
Fragments arranged by size:
2422 1451 936 635 591 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5172 5570 5844 5991 6402 5172
Size: 398 274 147 411 5509
Fragments arranged by size:
5509 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6514
Fragments arranged by size:
6514 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2174 1186
Size: 988 5751
BseYI C'CCAG_C
Cuts at: 2068 3286 3464 5942 6438 2068
Size: 1218 178 2478 496 2369
Fragments arranged by size:
2478 2369 1218 496 178
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2135 3291 1163
Size: 573 399 1156 4611
Fragments arranged by size:
4611 1156 573 399
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4189 4950 5978 1540
Size: 275 186 2188 761 1028 2301
Fragments arranged by size:
2301 2188 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2685 2929 4800 4885 5956 2685
Size: 244 1871 85 1071 3468
Fragments arranged by size:
3468 1871 1071 244 85
BsmI GAATG_Cn'
Cuts at: 3804 3979 4250 4343 3804
Size: 175 271 93 6200
Fragments arranged by size:
6200 271 175 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2016 2016
Size: 6739
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2102 2454 2685 2929 3285 3477 3992
Size: 399 312 352 231 244 356 192 515
Cuts at: 3992 4058 4800 4885 5956 1391
Size: 66 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 515 399 356 352 312
244 231 192 85 66
BspEI T'CCGG_A
Cuts at: 6402 6402
Size: 6739
BspHI T'CATG_A
Cuts at: 2260 4628 6358 2260
Size: 2368 1730 2641
Fragments arranged by size:
2641 2368 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2238 4094 844
Size: 1394 1856 3489
Fragments arranged by size:
3489 1856 1394
BsrBI CCG'CTC
Cuts at: 1394 1823 4626 1394
Size: 429 2803 3507
Fragments arranged by size:
3507 2803 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3450 3858 5234 5408 65
Size: 1183 2202 408 1376 174 1396
Fragments arranged by size:
2202 1396 1376 1183 408 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5383 5570 1700
Size: 110 3573 187 2869
Fragments arranged by size:
3573 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6739
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6739
BssSI C'ACGA_G
Cuts at: 2117 4799 5811 2117
Size: 2682 1012 3045
Fragments arranged by size:
3045 2682 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2185 2645 794
Size: 1391 460 4888
Fragments arranged by size:
4888 1391 460
BstBI TT'CG_AA
Cuts at: 5543 5543
Size: 6739
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6739
BstXI CCAn_nnnn'nTGG
Cuts at: 3089 3089
Size: 6739
BstYI r'GATC_y
Cuts at: 1972 4069 4436 4821 4838 6290 6376 6388 6734
Size: 2097 367 385 17 1452 86 12 346
Cuts at: 6734 1972
Size: 1977
Fragments arranged by size:
2097 1977 1452 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 4036 4036
Size: 6739
Bsu36I CC'TnA_GG
Cuts at: 2157 2157
Size: 6739
BtgI C'CryG_G
Cuts at: 513 1065 2004 3504 513
Size: 552 939 1500 3748
Fragments arranged by size:
3748 1500 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6000
Fragments arranged by size:
6000 683 56
BtsI GCAGTG_nn'
Cuts at: 2835 2844 4255 5049 5069 2835
Size: 9 1411 794 20 4505
Fragments arranged by size:
4505 1411 794 20 9
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6739
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6704
DraI TTT'AAA
Cuts at: 4043 4043
Size: 6739
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2405 5746 809
Size: 507 1089 3341 1802
Fragments arranged by size:
3341 1802 1089 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2542 4186 5075
Size: 54 1425 50 275 18 712 1644 889
Cuts at: 5075 6704 8
Size: 1629 43
Fragments arranged by size:
1644 1629 1425 889 712 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4186 1537
Size: 275 2374 4090
Fragments arranged by size:
4090 2374 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2466 2736 2856 3783 4669 1353
Size: 1113 270 120 927 886 3423
Fragments arranged by size:
3423 1113 927 886 270 120
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5326 5700 5846 387
Size: 1064 138 3737 374 146 1280
Fragments arranged by size:
3737 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2683 2683
Size: 6739
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2399 2627 2661 2880 2952 3284 3536
Size: 393 767 228 34 219 72 332 252
Cuts at: 3536 4802 5399 6186 6454 1239
Size: 1266 597 787 268 1524
Fragments arranged by size:
1524 1266 787 767 597 393 332 268
252 228 219 72 34
EcoNI CCTnn'n_nnAGG
Cuts at: 2234 2234
Size: 6739
EcoO109I rG'GnC_Cy
Cuts at: 2098 2099 2098
Size: 1 6738
EcoRI G'AATT_C
Cuts at: 4048 4426 4048
Size: 378 6361
FspI TGC'GCA
Cuts at: 5245 5245
Size: 6739
HaeII r_GCGC'y
Cuts at: 1678 2127 2939 5886 1678
Size: 449 812 2947 2531
Fragments arranged by size:
2947 2531 812 449
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2359 2391 2956 2988 3379
Size: 32 135 32 787 32 565 32 391
Cuts at: 3379 3411 3803 3835 5380 5412 5454 5486 1373
Size: 32 392 32 1545 32 42 32 2626
Fragments arranged by size:
2626 1545 787 565 392 391 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3973 4083 4329 669
Size: 988 281 2035 110 246 3079
Fragments arranged by size:
3079 2035 988 281 246 110
HindIII A'AGCT_T
Cuts at: 748 3580 748
Size: 2832 3907
HpaI GTT'AAC
Cuts at: 4329 4329
Size: 6739
KasI G'GCGC_C
Cuts at: 2123 2123
Size: 6739
KpnI G_GTAC'C
Cuts at: 4064 4064
Size: 6739
MfeI C'AATT_G
Cuts at: 4338 4338
Size: 6739
MluI A'CGCG_T
Cuts at: 6615 6615
Size: 6739
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3416 5853 6037 1305
Size: 2111 2437 184 2007
Fragments arranged by size:
2437 2111 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2544 10
Size: 54 1425 343 712 4205
Fragments arranged by size:
4205 1425 712 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3357 4697 5056 5215 518
Size: 648 2191 1340 359 159 2042
Fragments arranged by size:
2191 2042 1340 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2182 2191 3290 3413 4155 4832 5980 6225
Size: 576 9 1099 123 742 677 1148 245
Cuts at: 6225 1606
Size: 2120
Fragments arranged by size:
2120 1148 1099 742 677 576 245 123
9
NarI GG'CG_CC
Cuts at: 2124 2124
Size: 6739
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5248
Fragments arranged by size:
5248 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6739
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6739
NotI GC'GGCC_GC
Cuts at: 4186 4186
Size: 6739
NspI r_CATG'y
Cuts at: 1132 1229 3427 4097 5642 1132
Size: 97 2198 670 1545 2229
Fragments arranged by size:
2229 2198 1545 670 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6739
PciI A'CATG_T
Cuts at: 5638 5638
Size: 6739
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3878 3953 1108
Size: 2770 75 3894
Fragments arranged by size:
3894 2770 75
PfoI T'CCnGG_A
Cuts at: 2929 2929
Size: 6739
PmeI GTTT'AAAC
Cuts at: 4043 4043
Size: 6739
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4808 4840 6321 6353 4808
Size: 32 1481 32 5194
Fragments arranged by size:
5194 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6739
PsiI TTA'TAA
Cuts at: 4309 4309
Size: 6739
PspOMI G'GGCC_C
Cuts at: 2098 2098
Size: 6739
PstI C_TGCA'G
Cuts at: 830 2181 4091 830
Size: 1351 1910 3478
Fragments arranged by size:
3478 1910 1351
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6739
PvuII CAG'CTG
Cuts at: 3290 3413 3290
Size: 123 6616
SacI G_AGCT'C
Cuts at: 2685 2685
Size: 6739
SalI G'TCGA_C
Cuts at: 1936 4081 1936
Size: 2145 4594
SapI GCTCTTCn'nnn_
Cuts at: 2736 2736
Size: 6739
SbfI CC_TGCA'GG
Cuts at: 4091 4091
Size: 6739
ScaI AGT'ACT
Cuts at: 4987 6699 4987
Size: 1712 5027
SfcI C'TryA_G
Cuts at: 826 1045 2177 2527 2728 3074 4087 5222 5903
Size: 219 1132 350 201 346 1013 1135 681
Cuts at: 5903 6094 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1132 1013 681 350 346 219
201 191
SfoI GGC'GCC
Cuts at: 2125 2125
Size: 6739
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6739
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6739
SmaI CCC'GGG
Cuts at: 1872 4066 1872
Size: 2194 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4843 5744 6006 820
Size: 197 926 2900 901 262 1553
Fragments arranged by size:
2900 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4530 493
Size: 4037 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6739
SphI G_CATG'C
Cuts at: 1132 4097 1132
Size: 2965 3774
SspI AAT'ATT
Cuts at: 5 52 2291 2381 2506 3247 4663 5
Size: 47 2239 90 125 741 1416 2081
Fragments arranged by size:
2239 2081 1416 741 125 90 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6739
StyI C'CwwG_G
Cuts at: 513 1065 2004 4178 513
Size: 552 939 2174 3074
Fragments arranged by size:
3074 2174 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4766 4783 4936 5121 5566 325
Size: 1255 3186 17 153 185 445 1498
Fragments arranged by size:
3186 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2948 3025 4985
Size: 274 80 33 51 1443 971 77 1960
Cuts at: 4985 6697 96
Size: 1712 138
Fragments arranged by size:
1960 1712 1443 971 274 138 80 77
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6699 6731 6699
Size: 32 6707
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6739
XbaI T'CTAG_A
Cuts at: 4075 4075
Size: 6739
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3511 3511
Size: 6739
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6739
XmaI C'CCGG_G
Cuts at: 1870 4064 1870
Size: 2194 4545
XmnI GAAnn'nnTTC
Cuts at: 2487 4868 2487
Size: 2381 4358
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4547 276
Size: 53 83 186 936 3013 2468
Fragments arranged by size:
3013 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI DraIII EcoRV FalI FseI FspAI NaeI NgoMIV
NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII
SacII SanDI SexAI SfiI SrfI SwaI