Restriction map for
pFN21AA1568
(Circular) (Six-base) MAPSORT of: pFN21AA1568.seq Check: 4558 from: 1 to: 8839
pFN21AA1568 8839 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6649 278
Size: 53 83 186 936 5113 2468
Fragments arranged by size:
5113 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2606 6182 1937
Size: 669 3576 4594
Fragments arranged by size:
4594 3576 669
Acc65I G'GTAC_C
Cuts at: 2618 3638 6160 2618
Size: 1020 2522 5297
Fragments arranged by size:
5297 2522 1020
AclI AA'CG_TT
Cuts at: 1563 3151 6966 7339 1563
Size: 1588 3815 373 3063
Fragments arranged by size:
3815 3063 1588 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4014 4221 6902 8286 8554 1632
Size: 2382 207 2681 1384 268 1917
Fragments arranged by size:
2681 2382 1917 1384 268 207
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8642
AflIII A'CryG_T
Cuts at: 1203 7738 8715 1203
Size: 6535 977 1327
Fragments arranged by size:
6535 1327 977
AgeI A'CCGG_T
Cuts at: 7670 7670
Size: 8839
AhdI GACnn_n'nnGTC
Cuts at: 4032 7568 4032
Size: 3536 5303
AleI CACnn'nnGTG
Cuts at: 1166 4565 1166
Size: 3399 5440
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5469 1184
Size: 4285 4554
AlwNI CAG_nnn'CTG
Cuts at: 3527 3839 6582 8154 3527
Size: 312 2743 1572 4212
Fragments arranged by size:
4212 2743 1572 312
ApaLI G'TGCA_C
Cuts at: 2291 6896 8052 2291
Size: 4605 1156 3078
Fragments arranged by size:
4605 3078 1156
ApoI r'AATT_y
Cuts at: 1493 2732 3433 4060 4843 6148 6374 6526 8635
Size: 1239 701 627 783 1305 226 152 2109
Cuts at: 8635 1493
Size: 1697
Fragments arranged by size:
2109 1697 1305 1239 783 701 627 226
152
AseI AT'TA_AT
Cuts at: 160 1032 7393 160
Size: 872 6361 1606
Fragments arranged by size:
6361 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8839
AvaI C'yCGr_G
Cuts at: 1870 1943 2475 6164 1870
Size: 73 532 3689 4545
Fragments arranged by size:
4545 3689 532 73
AvrII C'CTAG_G
Cuts at: 6032 6032
Size: 8839
BamHI G'GATC_C
Cuts at: 2267 4285 5201 5729 6020 6169 6536 2267
Size: 2018 916 528 291 149 367 4570
Fragments arranged by size:
4570 2018 916 528 367 291 149
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2618 2870 3638 4604 5316
Size: 325 217 627 831 252 768 966 712
Cuts at: 5316 5950 6160 7615 618
Size: 634 210 1455 1842
Fragments arranged by size:
1842 1455 966 831 768 712 634 627
325 252 217 210
BanII G_rGCy'C
Cuts at: 1391 2145 2941 6107 6158 1391
Size: 754 796 3166 51 4072
Fragments arranged by size:
4072 3166 796 754 51
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2066 3917 4733 928
Size: 971 167 1851 816 5034
Fragments arranged by size:
5034 1851 971 816 167
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7030 7064 1903
Size: 34 5093 34 3678
Fragments arranged by size:
5093 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2561 6731 7941 2561
Size: 4170 1210 3459
Fragments arranged by size:
4170 3459 1210
BclI T'GATC_A
Cuts at: 1548 5119 1548
Size: 3571 5268
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7450 136
Size: 107 122 71 631 605 5778 1525
Fragments arranged by size:
5778 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4332 5007 5089 8834 4332
Size: 675 82 3745 4337
Fragments arranged by size:
4337 3745 675 82
BlpI GC'TnA_GC
Cuts at: 6256 6256
Size: 8839
Bme1580I G_kGCm'C
Cuts at: 1790 2295 4219 5321 5955 6900 8056 1790
Size: 505 1924 1102 634 945 1156 2573
Fragments arranged by size:
2573 1924 1156 1102 945 634 505
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8839
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3985 5231 5961
Size: 416 53 83 399 24 2568 1246 730
Cuts at: 5961 6570 7528 442
Size: 609 958 1753
Fragments arranged by size:
2568 1753 1246 958 730 609 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8839
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2463 2480 2735 3144 3242 3520 3549 4281
Size: 1224 17 255 409 98 278 29 732
Cuts at: 4281 5577 5640 5964 7499 1239
Size: 1296 63 324 1535 2579
Fragments arranged by size:
2579 1535 1296 1224 732 409 324 278
255 98 63 29 17
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2967 6964 7829 8127 2967
Size: 3997 865 298 3679
Fragments arranged by size:
3997 3679 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3120 3815 7502 882
Size: 617 1621 695 3687 2219
Fragments arranged by size:
3687 2219 1621 695 617
BsaAI yAC'GTr
Cuts at: 493 1204 6630 493
Size: 711 5426 2702
Fragments arranged by size:
5426 2702 711
BsaBI GATnn'nnATC
Cuts at: 8833 8833
Size: 8839
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6646 7028 7663 275
Size: 53 83 186 936 5113 382 635 1451
Fragments arranged by size:
5113 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 7272 7670 7944 8091 8502 7272
Size: 398 274 147 411 7609
Fragments arranged by size:
7609 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 5449 5479 1178
Size: 30 165 30 4046 30 4538
Fragments arranged by size:
4538 4046 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4281 4284 4716 5770 5954 1186
Size: 3095 3 432 1054 184 4071
Fragments arranged by size:
4071 3095 1054 432 184 3
BseYI C'CCAG_C
Cuts at: 2261 2650 3087 3274 4611 4760 8042 8538 2261
Size: 389 437 187 1337 149 3282 496 2562
Fragments arranged by size:
3282 2562 1337 496 437 389 187 149
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3525 3914 4482 5853 1163
Size: 573 1789 389 568 1371 4149
Fragments arranged by size:
4149 1789 1371 573 568 389
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6289 7050 8078 1540
Size: 275 186 4288 761 1028 2301
Fragments arranged by size:
4288 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2295 2941 5713 6900 6985 8056 2295
Size: 646 2772 1187 85 1071 3078
Fragments arranged by size:
3078 2772 1187 1071 646 85
BsmI GAATG_Cn'
Cuts at: 5014 6350 6443 5014
Size: 1336 93 7410
Fragments arranged by size:
7410 1336 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2134 2382 4501 2134
Size: 248 2119 6472
Fragments arranged by size:
6472 2119 248
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2145 2295 2941 4219 5321 5713 5955
Size: 399 355 150 646 1278 1102 392 242
Cuts at: 5955 6107 6158 6900 6985 8056 1391
Size: 152 51 742 85 1071 2174
Fragments arranged by size:
2174 1278 1102 1071 742 646 399 392
355 242 152 150 85 51
BspEI T'CCGG_A
Cuts at: 8502 8502
Size: 8839
BspHI T'CATG_A
Cuts at: 6728 8458 6728
Size: 1730 7109
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3573 3580 6194 844
Size: 2729 7 2614 3489
Fragments arranged by size:
3489 2729 2614 7
BsrBI CCG'CTC
Cuts at: 1394 1823 6726 1394
Size: 429 4903 3507
Fragments arranged by size:
4903 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4519 4729 5324 5906 6076 7334 7508
Size: 1183 3271 210 595 582 170 1258 174
Cuts at: 7508 65
Size: 1396
Fragments arranged by size:
3271 1396 1258 1183 595 582 210 174
170
BsrFI r'CCGG_y
Cuts at: 1700 1810 2172 7483 7670 1700
Size: 110 362 5311 187 2869
Fragments arranged by size:
5311 2869 362 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8839
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8839
BssSI C'ACGA_G
Cuts at: 4795 6899 7911 4795
Size: 2104 1012 5723
Fragments arranged by size:
5723 2104 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5494 794
Size: 4700 4139
BstBI TT'CG_AA
Cuts at: 2521 7643 2521
Size: 5122 3717
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8839
BstXI CCAn_nnnn'nTGG
Cuts at: 2629 5726 2629
Size: 3097 5742
BstYI r'GATC_y
Cuts at: 1972 2267 2755 3388 3835 4285 4332 5007 5089
Size: 295 488 633 447 450 47 675 82
Cuts at: 5089 5201 5729 6020 6169 6536 6921 6938 8390
Size: 112 528 291 149 367 385 17 1452
Cuts at: 8390 8476 8488 8834 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 675 633 528 488 450 447
385 367 346 295 291 149 112 86
82 47 17 12
BstZ17I GTA'TAC
Cuts at: 2607 2607
Size: 8839
BtgI C'CryG_G
Cuts at: 513 1065 2096 2554 3714 3954 4769 4852 5474
Size: 552 1031 458 1160 240 815 83 622
Cuts at: 5474 513
Size: 3878
Fragments arranged by size:
3878 1160 1031 815 622 552 458 240
83
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8100
Fragments arranged by size:
8100 683 56
BtsI GCAGTG_nn'
Cuts at: 2367 3300 3419 3513 5283 5451 6355 7149 7169
Size: 933 119 94 1770 168 904 794 20
Cuts at: 7169 2367
Size: 4037
Fragments arranged by size:
4037 1770 933 904 794 168 119 94
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8839
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2410 2445 529
Size: 35 1846 35 6923
Fragments arranged by size:
6923 1846 35 35
DraI TTT'AAA
Cuts at: 6143 6143
Size: 8839
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7846 809
Size: 507 6530 1802
Fragments arranged by size:
6530 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2174 2960 4142
Size: 54 1425 50 275 18 344 786 1182
Cuts at: 4142 4787 4849 5986 6286 7175 8804 8
Size: 645 62 1137 300 889 1629 43
Fragments arranged by size:
1629 1425 1182 1137 889 786 645 344
300 275 62 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6286 1537
Size: 275 4474 4090
Fragments arranged by size:
4474 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3345 4655 5182 5475 5670 6769 1353
Size: 1992 1310 527 293 195 1099 3423
Fragments arranged by size:
3423 1992 1310 1099 527 293 195
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2180 5902 7426 7800 7946 387
Size: 1064 138 591 3722 1524 374 146 1280
Fragments arranged by size:
3722 1524 1280 1064 591 374 146 138
EcoICRI GAG'CTC
Cuts at: 2939 2939
Size: 8839
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2463 2480 2735 3144 3242 3520 3549
Size: 393 831 17 255 409 98 278 29
Cuts at: 3549 4014 4221 4281 5577 5640 5964 6902 7499
Size: 465 207 60 1296 63 324 938 597
Cuts at: 7499 8286 8554 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1296 938 831 787 597 465 409
393 324 278 268 255 207 98 63
60 29 17
EcoNI CCTnn'n_nnAGG
Cuts at: 2316 4112 5406 2316
Size: 1796 1294 5749
Fragments arranged by size:
5749 1796 1294
EcoO109I rG'GnC_Cy
Cuts at: 5389 5700 5389
Size: 311 8528
EcoRI G'AATT_C
Cuts at: 6148 6526 6148
Size: 378 8461
EcoRV GAT'ATC
Cuts at: 3856 3856
Size: 8839
FseI GG_CCGG'CC
Cuts at: 2176 2176
Size: 8839
FspI TGC'GCA
Cuts at: 2285 3845 7345 2285
Size: 1560 3500 3779
Fragments arranged by size:
3779 3500 1560
FspAI rTGC'GCAy
Cuts at: 3845 3845
Size: 8839
HaeII r_GCGC'y
Cuts at: 1678 7986 1678
Size: 6308 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2225 2257 2332 2364 2965
Size: 32 135 32 653 32 75 32 601
Cuts at: 2965 2997 3522 3554 3964 3996 4006 4018 4038
Size: 32 525 32 410 32 10 12 20
Cuts at: 4038 4050 4163 4195 4324 4350 4356 4382 5833
Size: 12 113 32 129 26 6 26 1451
Cuts at: 5833 5865 7480 7512 7554 7586 1373
Size: 32 1615 32 42 32 2626
Fragments arranged by size:
2626 1615 1451 653 601 525 410 135
129 113 75 42 32 32 32 32
32 32 32 32 32 32 32 26
26 20 12 12 10 6
HincII GTy'rAC
Cuts at: 669 1657 1938 4105 5520 6183 6429 669
Size: 988 281 2167 1415 663 246 3079
Fragments arranged by size:
3079 2167 1415 988 663 281 246
HindIII A'AGCT_T
Cuts at: 748 5682 748
Size: 4934 3905
HpaI GTT'AAC
Cuts at: 6429 6429
Size: 8839
KpnI G_GTAC'C
Cuts at: 2622 3642 6164 2622
Size: 1020 2522 5297
Fragments arranged by size:
5297 2522 1020
MfeI C'AATT_G
Cuts at: 6438 6438
Size: 8839
MluI A'CGCG_T
Cuts at: 8715 8715
Size: 8839
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2434 2881 3180 4228 4450 5043 6132 7953
Size: 1129 447 299 1048 222 593 1089 1821
Cuts at: 7953 8137 1305
Size: 184 2007
Fragments arranged by size:
2007 1821 1129 1089 1048 593 447 299
222 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4789 5988 10
Size: 54 1425 343 2957 1199 2861
Fragments arranged by size:
2957 2861 1425 1199 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2876 4051 4308 4565 5511 6797 7156
Size: 648 1710 1175 257 257 946 1286 359
Cuts at: 7156 7315 518
Size: 159 2042
Fragments arranged by size:
2042 1710 1286 1175 946 648 359 257
257 159
MspA1I CmG'CkG
Cuts at: 1606 2098 2265 2441 3035 3705 6255 6932 8080
Size: 492 167 176 594 670 2550 677 1148
Cuts at: 8080 8325 1606
Size: 245 2120
Fragments arranged by size:
2550 2120 1148 677 670 594 492 245
176 167
NaeI GCC'GGC
Cuts at: 2174 2174
Size: 8839
NcoI C'CATG_G
Cuts at: 513 1065 4769 513
Size: 552 3704 4583
Fragments arranged by size:
4583 3704 552
NdeI CA'TA_TG
Cuts at: 387 4637 4993 387
Size: 4250 356 4233
Fragments arranged by size:
4250 4233 356
NgoMIV G'CCGG_C
Cuts at: 2172 2172
Size: 8839
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8839
NotI GC'GGCC_GC
Cuts at: 6286 6286
Size: 8839
NspI r_CATG'y
Cuts at: 1132 1229 6197 7742 1132
Size: 97 4968 1545 2229
Fragments arranged by size:
4968 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8839
PciI A'CATG_T
Cuts at: 7738 7738
Size: 8839
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2193 2502 3289 4168 5356 1108
Size: 1085 309 787 879 1188 4591
Fragments arranged by size:
4591 1188 1085 879 787 309
PfoI T'CCnGG_A
Cuts at: 2457 3497 5725 2457
Size: 1040 2228 5571
Fragments arranged by size:
5571 2228 1040
PmeI GTTT'AAAC
Cuts at: 6143 6143
Size: 8839
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6908 6940 8421 8453 6908
Size: 32 1481 32 7294
Fragments arranged by size:
7294 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 5389 5389
Size: 8839
PshAI GACnn'nnGTC
Cuts at: 1613 3822 5446 1613
Size: 2209 1624 5006
Fragments arranged by size:
5006 2209 1624
PsiI TTA'TAA
Cuts at: 6409 6409
Size: 8839
PstI C_TGCA'G
Cuts at: 830 3426 4041 5502 6191 830
Size: 2596 615 1461 689 3478
Fragments arranged by size:
3478 2596 1461 689 615
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8839
PvuII CAG'CTG
Cuts at: 2441 3035 3705 2441
Size: 594 670 7575
Fragments arranged by size:
7575 670 594
RsrII CG'GwC_CG
Cuts at: 4395 4395
Size: 8839
SacI G_AGCT'C
Cuts at: 2941 2941
Size: 8839
SacII CC_GC'GG
Cuts at: 2099 2099
Size: 8839
SalI G'TCGA_C
Cuts at: 1936 6181 1936
Size: 4245 4594
SanDI GG'GwC_CC
Cuts at: 5389 5389
Size: 8839
SbfI CC_TGCA'GG
Cuts at: 6191 6191
Size: 8839
ScaI AGT'ACT
Cuts at: 7087 8799 7087
Size: 1712 7127
SexAI A'CCwGG_T
Cuts at: 2703 2703
Size: 8839
SfcI C'TryA_G
Cuts at: 826 1045 2727 3422 4037 5498 5956 6187 7322
Size: 219 1682 695 615 1461 458 231 1135
Cuts at: 7322 8003 8194 826
Size: 681 191 1471
Fragments arranged by size:
1682 1471 1461 1135 695 681 615 458
231 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8839
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8839
SmaI CCC'GGG
Cuts at: 1872 2477 6166 1872
Size: 605 3689 4545
Fragments arranged by size:
4545 3689 605
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2982 6943 7844 8106 820
Size: 197 926 1039 3961 901 262 1553
Fragments arranged by size:
3961 1553 1039 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6630 493
Size: 6137 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8839
SphI G_CATG'C
Cuts at: 1132 6197 1132
Size: 5065 3774
SspI AAT'ATT
Cuts at: 5 52 6763 5
Size: 47 6711 2081
Fragments arranged by size:
6711 2081 47
StuI AGG'CCT
Cuts at: 1517 5857 5945 1517
Size: 4340 88 4411
Fragments arranged by size:
4411 4340 88
StyI C'CwwG_G
Cuts at: 513 1065 2749 3805 4000 4162 4769 4882 5350
Size: 552 1684 1056 195 162 607 113 468
Cuts at: 5350 5946 6032 6278 513
Size: 596 86 246 3074
Fragments arranged by size:
3074 1684 1056 607 596 552 468 246
195 162 113 86
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2499 4383 6866 6883 7036 7221 7666
Size: 1255 919 1884 2483 17 153 185 445
Cuts at: 7666 325
Size: 1498
Fragments arranged by size:
2483 1884 1498 1255 919 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2858 4237 5332
Size: 274 80 33 51 1443 881 1379 1095
Cuts at: 5332 7085 8797 96
Size: 1753 1712 138
Fragments arranged by size:
1753 1712 1443 1379 1095 881 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2916 2948 3319 3351 8799 8831 2916
Size: 32 371 32 5448 32 2924
Fragments arranged by size:
5448 2924 371 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3523 1316
Size: 2207 6632
XbaI T'CTAG_A
Cuts at: 5746 6175 5746
Size: 429 8410
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2772 3039 3286 5249 2772
Size: 267 247 1963 6362
Fragments arranged by size:
6362 1963 267 247
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8839
XmaI C'CCGG_G
Cuts at: 1870 2475 6164 1870
Size: 605 3689 4545
Fragments arranged by size:
4545 3689 605
XmnI GAAnn'nnTTC
Cuts at: 6007 6968 6007
Size: 961 7878
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6647 276
Size: 53 83 186 936 5113 2468
Fragments arranged by size:
5113 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI Bsu36I DraIII FalI KasI
NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI
SapI SfiI SfoI SrfI SwaI