Restriction map for
pFN21AA1567
(Circular) (Six-base) MAPSORT of: pFN21AA1567.seq Check: 8629 from: 1 to: 7288
pFN21AA1567 7288 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5098 278
Size: 53 83 186 936 3562 2468
Fragments arranged by size:
3562 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3115 4631 1937
Size: 1178 1516 4594
Fragments arranged by size:
4594 1516 1178
Acc65I G'GTAC_C
Cuts at: 4609 4609
Size: 7288
AclI AA'CG_TT
Cuts at: 1563 5415 5788 1563
Size: 3852 373 3063
Fragments arranged by size:
3852 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2378 3695 5351 6735 7003 1632
Size: 746 1317 1656 1384 268 1917
Fragments arranged by size:
1917 1656 1384 1317 746 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7091
AflIII A'CryG_T
Cuts at: 1203 2271 3580 6187 7164 1203
Size: 1068 1309 2607 977 1327
Fragments arranged by size:
2607 1327 1309 1068 977
AgeI A'CCGG_T
Cuts at: 6119 6119
Size: 7288
AhdI GACnn_n'nnGTC
Cuts at: 6017 6017
Size: 7288
AleI CACnn'nnGTG
Cuts at: 1166 2501 4214 4379 1166
Size: 1335 1713 165 4075
Fragments arranged by size:
4075 1713 1335 165
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3324 1184
Size: 2140 5148
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3476 3508 4276 4308 3476
Size: 32 768 32 6456
Fragments arranged by size:
6456 768 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3934 4354 4415 5031 6603 3934
Size: 420 61 616 1572 4619
Fragments arranged by size:
4619 1572 616 420 61
ApaLI G'TGCA_C
Cuts at: 4387 5345 6501 4387
Size: 958 1156 5174
Fragments arranged by size:
5174 1156 958
ApoI r'AATT_y
Cuts at: 1493 2744 3167 4275 4597 4823 4975 7084 1493
Size: 1251 423 1108 322 226 152 2109 1697
Fragments arranged by size:
2109 1697 1251 1108 423 322 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5842 160
Size: 872 4810 1606
Fragments arranged by size:
4810 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7288
AvaI C'yCGr_G
Cuts at: 1870 1943 2996 4613 1870
Size: 73 1053 1617 4545
Fragments arranged by size:
4545 1617 1053 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2193 2226 2193
Size: 33 7255
BamHI G'GATC_C
Cuts at: 4522 4618 4985 4522
Size: 96 367 6825
Fragments arranged by size:
6825 367 96
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4609 6064 618
Size: 325 217 627 2822 1455 1842
Fragments arranged by size:
2822 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2656 3204 3906 4607 1391
Size: 1265 548 702 701 4072
Fragments arranged by size:
4072 1265 702 701 548
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3647 928
Size: 971 1748 4569
Fragments arranged by size:
4569 1748 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5479 5513 1903
Size: 34 3542 34 3678
Fragments arranged by size:
3678 3542 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5180 6390 5180
Size: 1210 6078
BclI T'GATC_A
Cuts at: 1548 3066 1548
Size: 1518 5770
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 5899 136
Size: 107 122 71 631 605 337 3890 1525
Fragments arranged by size:
3890 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 3083 3891 7283 3083
Size: 808 3392 3088
Fragments arranged by size:
3392 3088 808
BlpI GC'TnA_GC
Cuts at: 2655 4705 2655
Size: 2050 5238
Bme1580I G_kGCm'C
Cuts at: 1790 2424 4391 5349 6505 1790
Size: 634 1967 958 1156 2573
Fragments arranged by size:
2573 1967 1156 958 634
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7288
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2495 5019 5977
Size: 416 53 83 399 24 1078 2524 958
Cuts at: 5977 442
Size: 1753
Fragments arranged by size:
2524 1753 1078 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2037 1056
Size: 981 6307
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4407 4407
Size: 7288
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4331 5948 1239
Size: 3092 1617 2579
Fragments arranged by size:
3092 2579 1617
Bpu10I CC'TnA_GC
Cuts at: 4539 4539
Size: 7288
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5413 6278 6576 5413
Size: 865 298 6125
Fragments arranged by size:
6125 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2587 2771 5951 882
Size: 617 1088 184 3180 2219
Fragments arranged by size:
3180 2219 1088 617 184
BsaAI yAC'GTr
Cuts at: 493 1204 5079 493
Size: 711 3875 2702
Fragments arranged by size:
3875 2702 711
BsaBI GATnn'nnATC
Cuts at: 2136 4470 7282 2136
Size: 2334 2812 2142
Fragments arranged by size:
2812 2334 2142
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5095 5477 6112 275
Size: 53 83 186 936 3562 382 635 1451
Fragments arranged by size:
3562 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5721 6119 6393 6540 6951 5721
Size: 398 274 147 411 6058
Fragments arranged by size:
6058 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2468 2498 2555 2585 3476
Size: 30 165 30 1065 30 57 30 891
Cuts at: 3476 3506 3756 3786 1178
Size: 30 250 30 4680
Fragments arranged by size:
4680 1065 891 250 165 57 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3896 1186
Size: 2710 4578
BseYI C'CCAG_C
Cuts at: 2014 2417 2733 3784 3845 4566 6491 6987 2014
Size: 403 316 1051 61 721 1925 496 2315
Fragments arranged by size:
2315 1925 1051 721 496 403 316 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3492 3893 4182 1163
Size: 573 1756 401 289 4269
Fragments arranged by size:
4269 1756 573 401 289
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4519 4738 5499 6527 1540
Size: 275 186 2518 219 761 1028 2301
Fragments arranged by size:
2518 2301 1028 761 275 219 186
BsiHKAI G_wGCw'C
Cuts at: 3204 3906 4391 5349 5434 6505 3204
Size: 702 485 958 85 1071 3987
Fragments arranged by size:
3987 1071 958 702 485 85
BsmI GAATG_Cn'
Cuts at: 3432 4799 4892 3432
Size: 1367 93 5828
Fragments arranged by size:
5828 1367 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2424 2656 3204 3906 4391 4607 5349
Size: 399 634 232 548 702 485 216 742
Cuts at: 5349 5434 6505 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 702 634 548 485 399
232 216 85
BspEI T'CCGG_A
Cuts at: 6951 6951
Size: 7288
BspHI T'CATG_A
Cuts at: 4474 5177 6907 4474
Size: 703 1730 4855
Fragments arranged by size:
4855 1730 703
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2605 4643 844
Size: 1761 2038 3489
Fragments arranged by size:
3489 2038 1761
BsrBI CCG'CTC
Cuts at: 1394 1823 3767 5175 1394
Size: 429 1944 1408 3507
Fragments arranged by size:
3507 1944 1408 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3810 4201 5783 5957 65
Size: 1183 2562 391 1582 174 1396
Fragments arranged by size:
2562 1582 1396 1183 391 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3849 4104 5932 6119 1700
Size: 110 2039 255 1828 187 2869
Fragments arranged by size:
2869 2039 1828 255 187 110
BsrGI T'GTAC_A
Cuts at: 96 2274 2891 96
Size: 2178 617 4493
Fragments arranged by size:
4493 2178 617
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7288
BssSI C'ACGA_G
Cuts at: 5348 6360 5348
Size: 1012 6276
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3086 3470 4424 794
Size: 2292 384 954 3658
Fragments arranged by size:
3658 2292 954 384
BstBI TT'CG_AA
Cuts at: 4072 6092 4072
Size: 2020 5268
BstEII G'GTnAC_C
Cuts at: 1184 2779 1184
Size: 1595 5693
BstYI r'GATC_y
Cuts at: 1972 3083 3891 4522 4618 4985 5370 5387 6839
Size: 1111 808 631 96 367 385 17 1452
Cuts at: 6839 6925 6937 7283 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1111 808 631 385 367 346
96 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4527 4527
Size: 7288
BtgI C'CryG_G
Cuts at: 513 1065 2004 4012 513
Size: 552 939 2008 3789
Fragments arranged by size:
3789 2008 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3040 487
Size: 683 56 1814 4735
Fragments arranged by size:
4735 1814 683 56
BtsI GCAGTG_nn'
Cuts at: 4332 4804 5598 5618 4332
Size: 472 794 20 6002
Fragments arranged by size:
6002 794 472 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7288
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7253
DraI TTT'AAA
Cuts at: 2359 4592 2359
Size: 2233 5055
DraIII CAC_nnn'GTG
Cuts at: 3470 3615 3617 3470
Size: 145 2 7141
Fragments arranged by size:
7141 145 2
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6295 809
Size: 507 4979 1802
Fragments arranged by size:
4979 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2834 3606 3838
Size: 54 1425 50 275 18 1004 772 232
Cuts at: 3838 4052 4106 4516 4735 5624 7253 8
Size: 214 54 410 219 889 1629 43
Fragments arranged by size:
1629 1425 1004 889 772 410 275 232
219 214 54 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4516 4735 1537
Size: 275 2704 219 4090
Fragments arranged by size:
4090 2704 275 219
EarI CTCTTCn'nnn_
Cuts at: 1353 2334 3099 3119 4160 5218 1353
Size: 981 765 20 1041 1058 3423
Fragments arranged by size:
3423 1058 1041 981 765 20
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5875 6249 6395 387
Size: 1064 138 4286 374 146 1280
Fragments arranged by size:
4286 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3202 3904 3202
Size: 702 6586
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2378 3695 4331 5351 5948 6735 7003
Size: 393 746 1317 636 1020 597 787 268
Cuts at: 7003 1239
Size: 1524
Fragments arranged by size:
1524 1317 1020 787 746 636 597 393
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2488 2550 4381 2488
Size: 62 1831 5395
Fragments arranged by size:
5395 1831 62
EcoO109I rG'GnC_Cy
Cuts at: 2018 3729 3931 2018
Size: 1711 202 5375
Fragments arranged by size:
5375 1711 202
EcoRI G'AATT_C
Cuts at: 4275 4597 4975 4275
Size: 322 378 6588
Fragments arranged by size:
6588 378 322
FspI TGC'GCA
Cuts at: 5794 5794
Size: 7288
HaeII r_GCGC'y
Cuts at: 1678 2015 6435 1678
Size: 337 4420 2531
Fragments arranged by size:
4420 2531 337
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2704 2736 3634 3666 3756
Size: 32 135 32 1132 32 898 32 90
Cuts at: 3756 3788 5929 5961 6003 6035 1373
Size: 32 2141 32 42 32 2626
Fragments arranged by size:
2626 2141 1132 898 135 90 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2185 4632 4878 669
Size: 988 281 247 2447 246 3079
Fragments arranged by size:
3079 2447 988 281 247 246
HindIII A'AGCT_T
Cuts at: 748 2409 748
Size: 1661 5627
HpaI GTT'AAC
Cuts at: 4878 4878
Size: 7288
KpnI G_GTAC'C
Cuts at: 4613 4613
Size: 7288
MfeI C'AATT_G
Cuts at: 2295 4887 2295
Size: 2592 4696
MluI A'CGCG_T
Cuts at: 7164 7164
Size: 7288
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2056 3565 6402 6586 1305
Size: 751 1509 2837 184 2007
Fragments arranged by size:
2837 2007 1509 751 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3608 3840 4054 10
Size: 54 1425 343 1776 232 214 3244
Fragments arranged by size:
3244 1776 1425 343 232 214 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2145 2427 2501 4214 4377 4379 4479
Size: 648 979 282 74 1713 163 2 100
Cuts at: 4479 5246 5605 5764 518
Size: 767 359 159 2042
Fragments arranged by size:
2042 1713 979 767 648 359 282 163
159 100 74 2
MspA1I CmG'CkG
Cuts at: 1606 3400 4366 4704 5381 6529 6774 1606
Size: 1794 966 338 677 1148 245 2120
Fragments arranged by size:
2120 1794 1148 966 677 338 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5797
Fragments arranged by size:
5797 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7288
NheI G'CTAG_C
Cuts at: 1052 2033 1052
Size: 981 6307
NotI GC'GGCC_GC
Cuts at: 4735 4735
Size: 7288
NspI r_CATG'y
Cuts at: 1132 1229 2275 3292 4083 4646 6191 1132
Size: 97 1046 1017 791 563 1545 2229
Fragments arranged by size:
2229 1545 1046 1017 791 563 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7288
PciI A'CATG_T
Cuts at: 2271 6187 2271
Size: 3916 3372
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2548 1108
Size: 1440 5848
PfoI T'CCnGG_A
Cuts at: 3927 3927
Size: 7288
PmeI GTTT'AAAC
Cuts at: 4592 4592
Size: 7288
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3476 3508 5357 5389 6870 6902 3476
Size: 32 1849 32 1481 32 3862
Fragments arranged by size:
3862 1849 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2018 3931 2018
Size: 1913 5375
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7288
PsiI TTA'TAA
Cuts at: 4858 4858
Size: 7288
PspXI vC'TCGA_Gb
Cuts at: 2996 2996
Size: 7288
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4335 4367 4335
Size: 32 7256
PstI C_TGCA'G
Cuts at: 830 4339 4640 830
Size: 3509 301 3478
Fragments arranged by size:
3509 3478 301
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7288
PvuII CAG'CTG
Cuts at: 3400 4366 3400
Size: 966 6322
SacI G_AGCT'C
Cuts at: 3204 3906 3204
Size: 702 6586
SalI G'TCGA_C
Cuts at: 1936 4630 1936
Size: 2694 4594
SanDI GG'GwC_CC
Cuts at: 2018 2018
Size: 7288
SapI GCTCTTCn'nnn_
Cuts at: 3119 3119
Size: 7288
SbfI CC_TGCA'GG
Cuts at: 4640 4640
Size: 7288
ScaI AGT'ACT
Cuts at: 5536 7248 5536
Size: 1712 5576
SexAI A'CCwGG_T
Cuts at: 4305 4305
Size: 7288
SfcI C'TryA_G
Cuts at: 826 1045 3075 3524 3910 4212 4261 4335 4636
Size: 219 2030 449 386 302 49 74 301
Cuts at: 4636 5771 6452 6643 826
Size: 1135 681 191 1471
Fragments arranged by size:
2030 1471 1135 681 449 386 302 301
219 191 74 49
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7288
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7288
SmaI CCC'GGG
Cuts at: 1872 4615 1872
Size: 2743 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2996 5392 6293 6555 820
Size: 197 926 1053 2396 901 262 1553
Fragments arranged by size:
2396 1553 1053 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5079 493
Size: 4586 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7288
SphI G_CATG'C
Cuts at: 1132 4646 1132
Size: 3514 3774
SspI AAT'ATT
Cuts at: 5 52 2232 2906 5212 5
Size: 47 2180 674 2306 2081
Fragments arranged by size:
2306 2180 2081 674 47
StuI AGG'CCT
Cuts at: 1517 4532 1517
Size: 3015 4273
StyI C'CwwG_G
Cuts at: 513 1065 2004 2065 4533 4727 513
Size: 552 939 61 2468 194 3074
Fragments arranged by size:
3074 2468 939 552 194 61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2365 3131 3283 5315 5332 5485 5670
Size: 1255 785 766 152 2032 17 153 185
Cuts at: 5670 6115 325
Size: 445 1498
Fragments arranged by size:
2032 1498 1255 785 766 445 185 153
152 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2274 2449 2891
Size: 274 80 33 51 1443 297 175 442
Cuts at: 2891 5534 7246 96
Size: 2643 1712 138
Fragments arranged by size:
2643 1712 1443 442 297 274 175 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2269 2301 2468 2500 3603 3635 7248 7280 2269
Size: 32 167 32 1103 32 3613 32 2277
Fragments arranged by size:
3613 2277 1103 167 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7288
XbaI T'CTAG_A
Cuts at: 4624 4624
Size: 7288
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 3817 2011
Size: 1806 5482
XhoI C'TCGA_G
Cuts at: 1943 2996 1943
Size: 1053 6235
XmaI C'CCGG_G
Cuts at: 1870 4613 1870
Size: 2743 4545
XmnI GAAnn'nnTTC
Cuts at: 5417 5417
Size: 7288
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5096 276
Size: 53 83 186 936 3562 2468
Fragments arranged by size:
3562 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI
PvuII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BbvCI BfrBI
BsiWI BsmBI BstXI BstZ17I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspOMI RsrII SacII SfiI SfoI SrfI SwaI