Restriction map for
pFN21AA1560
(Circular) (Six-base) MAPSORT of: pFN21AA1560.seq Check: 6823 from: 1 to: 7189
pFN21AA1560 7189 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4999 278
Size: 53 83 186 936 3463 2468
Fragments arranged by size:
3463 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3023 4532 1937
Size: 1086 1509 4594
Fragments arranged by size:
4594 1509 1086
Acc65I G'GTAC_C
Cuts at: 4510 4510
Size: 7189
AclI AA'CG_TT
Cuts at: 1563 5316 5689 1563
Size: 3753 373 3063
Fragments arranged by size:
3753 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2119 2751 2779 4142 4213 5252 6636 6904
Size: 487 632 28 1363 71 1039 1384 268
Cuts at: 6904 1632
Size: 1917
Fragments arranged by size:
1917 1384 1363 1039 632 487 268 71
28
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6992
AflIII A'CryG_T
Cuts at: 1203 2954 3342 6088 7065 1203
Size: 1751 388 2746 977 1327
Fragments arranged by size:
2746 1751 1327 977 388
AgeI A'CCGG_T
Cuts at: 3253 6020 3253
Size: 2767 4422
AhdI GACnn_n'nnGTC
Cuts at: 5918 5918
Size: 7189
AleI CACnn'nnGTG
Cuts at: 1166 2094 2387 1166
Size: 928 293 5968
Fragments arranged by size:
5968 928 293
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7189
AlwNI CAG_nnn'CTG
Cuts at: 3622 4250 4932 6504 3622
Size: 628 682 1572 4307
Fragments arranged by size:
4307 1572 682 628
ApaLI G'TGCA_C
Cuts at: 5246 6402 5246
Size: 1156 6033
ApoI r'AATT_y
Cuts at: 1493 2234 2619 2665 3586 4498 4724 4876 6985
Size: 741 385 46 921 912 226 152 2109
Cuts at: 6985 1493
Size: 1697
Fragments arranged by size:
2109 1697 921 912 741 385 226 152
46
AseI AT'TA_AT
Cuts at: 160 1032 3351 5743 160
Size: 872 2319 2392 1606
Fragments arranged by size:
2392 2319 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7189
AvaI C'yCGr_G
Cuts at: 1870 1943 2985 4514 1870
Size: 73 1042 1529 4545
Fragments arranged by size:
4545 1529 1042 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2233 2266 4238 4271 2233
Size: 33 1972 33 5151
Fragments arranged by size:
5151 1972 33 33
BamHI G'GATC_C
Cuts at: 4519 4886 4519
Size: 367 6822
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3893 4510 5965 618
Size: 325 217 627 2106 617 1455 1842
Fragments arranged by size:
2106 1842 1455 627 617 325 217
BanII G_rGCy'C
Cuts at: 1391 4508 1391
Size: 3117 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3531 3694 4089 928
Size: 971 1632 163 395 4028
Fragments arranged by size:
4028 1632 971 395 163
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5380 5414 1903
Size: 34 3443 34 3678
Fragments arranged by size:
3678 3443 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2261 5081 6291 2261
Size: 2820 1210 3159
Fragments arranged by size:
3159 2820 1210
BclI T'GATC_A
Cuts at: 1548 2559 3831 1548
Size: 1011 1272 4906
Fragments arranged by size:
4906 1272 1011
BfrBI ATG'CAT
Cuts at: 3607 3607
Size: 7189
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5800 136
Size: 107 122 71 631 605 4128 1525
Fragments arranged by size:
4128 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2138 2937 7184 2138
Size: 799 4247 2143
Fragments arranged by size:
4247 2143 799
BlpI GC'TnA_GC
Cuts at: 3411 4020 4606 3411
Size: 609 586 5994
Fragments arranged by size:
5994 609 586
Bme1580I G_kGCm'C
Cuts at: 1790 5250 6406 1790
Size: 3460 1156 2573
Fragments arranged by size:
3460 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 3345 1318
Size: 2027 5162
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2241 2580 3119
Size: 416 53 83 399 24 824 339 539
Cuts at: 3119 3309 3809 4920 5878 442
Size: 190 500 1111 958 1753
Fragments arranged by size:
1753 1111 958 824 539 500 416 399
339 190 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3435 1056
Size: 2379 4810
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2906 2906
Size: 7189
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3296 4211 5849 1239
Size: 2057 915 1638 2579
Fragments arranged by size:
2579 2057 1638 915
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2657 5314 6179 6477 2657
Size: 2657 865 298 3369
Fragments arranged by size:
3369 2657 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4379 5852 882
Size: 617 2880 1473 2219
Fragments arranged by size:
2880 2219 1473 617
BsaAI yAC'GTr
Cuts at: 493 1204 4980 493
Size: 711 3776 2702
Fragments arranged by size:
3776 2702 711
BsaBI GATnn'nnATC
Cuts at: 7183 7183
Size: 7189
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2326 4996 5378 6013
Size: 53 83 186 936 793 2670 382 635
Cuts at: 6013 275
Size: 1451
Fragments arranged by size:
2670 1451 936 793 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3253 3957 5622 6020 6294 6441 6852 3253
Size: 704 1665 398 274 147 411 3590
Fragments arranged by size:
3590 1665 704 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3269 3299 3866 3896 4158
Size: 30 165 30 1866 30 567 30 262
Cuts at: 4158 4188 1178
Size: 30 4179
Fragments arranged by size:
4179 1866 567 262 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3470 1186
Size: 2284 4905
BseYI C'CCAG_C
Cuts at: 2226 2472 3622 3680 6392 6888 2226
Size: 246 1150 58 2712 496 2527
Fragments arranged by size:
2712 2527 1150 496 246 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2001 2197 1163
Size: 573 265 196 6155
Fragments arranged by size:
6155 573 265 196
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4639 5400 6428 1540
Size: 275 186 2638 761 1028 2301
Fragments arranged by size:
2638 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2986 3987 4146 5250 5335 6406 2986
Size: 1001 159 1104 85 1071 3769
Fragments arranged by size:
3769 1104 1071 1001 159 85
BsmI GAATG_Cn'
Cuts at: 3966 4351 4700 4793 3966
Size: 385 349 93 6362
Fragments arranged by size:
6362 385 349 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2654 2654
Size: 7189
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2986 3987 4146 4508 5250 5335 6406
Size: 399 1196 1001 159 362 742 85 1071
Cuts at: 6406 1391
Size: 2174
Fragments arranged by size:
2174 1196 1071 1001 742 399 362 159
85
BspEI T'CCGG_A
Cuts at: 6852 6852
Size: 7189
BspHI T'CATG_A
Cuts at: 5078 6808 5078
Size: 1730 5459
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4544 844
Size: 3700 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5076 1394
Size: 429 3253 3507
Fragments arranged by size:
3507 3253 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2562 2760 3730 5684 5858 65
Size: 1183 1314 198 970 1954 174 1396
Fragments arranged by size:
1954 1396 1314 1183 970 198 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2551 3253 5833 6020 1700
Size: 110 741 702 2580 187 2869
Fragments arranged by size:
2869 2580 741 702 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7189
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7189
BssSI C'ACGA_G
Cuts at: 3558 5249 6261 3558
Size: 1691 1012 4486
Fragments arranged by size:
4486 1691 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3850 4267 4313 794
Size: 3056 417 46 3670
Fragments arranged by size:
3670 3056 417 46
BstBI TT'CG_AA
Cuts at: 3705 5993 3705
Size: 2288 4901
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7189
BstXI CCAn_nnnn'nTGG
Cuts at: 2816 2816
Size: 7189
BstYI r'GATC_y
Cuts at: 1972 2138 2696 2937 4519 4886 5271 5288 6740
Size: 166 558 241 1582 367 385 17 1452
Cuts at: 6740 6826 6838 7184 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1582 1452 558 385 367 346 241
166 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2308 2893 513
Size: 552 939 304 585 4809
Fragments arranged by size:
4809 939 585 552 304
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6450
Fragments arranged by size:
6450 683 56
BtsI GCAGTG_nn'
Cuts at: 3426 3902 4034 4705 5499 5519 3426
Size: 476 132 671 794 20 5096
Fragments arranged by size:
5096 794 671 476 132 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7189
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3445 3480 4213 4248 529
Size: 35 2881 35 733 35 3470
Fragments arranged by size:
3470 2881 733 35 35 35
DraI TTT'AAA
Cuts at: 2151 4493 2151
Size: 2342 4847
DraIII CAC_nnn'GTG
Cuts at: 3466 3466
Size: 7189
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3804 6196 809
Size: 507 2488 2392 1802
Fragments arranged by size:
2488 2392 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3861 4636 5525
Size: 54 1425 50 275 18 2031 775 889
Cuts at: 5525 7154 8
Size: 1629 43
Fragments arranged by size:
2031 1629 1425 889 775 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4636 1537
Size: 275 2824 4090
Fragments arranged by size:
4090 2824 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2738 5119 1353
Size: 1385 2381 3423
Fragments arranged by size:
3423 2381 1385
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5776 6150 6296 387
Size: 1064 138 4187 374 146 1280
Fragments arranged by size:
4187 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2119 2751 2779 3296 4142 4211 4213
Size: 393 487 632 28 517 846 69 2
Cuts at: 4213 5252 5849 6636 6904 1239
Size: 1039 597 787 268 1524
Fragments arranged by size:
1524 1039 846 787 632 597 517 487
393 268 69 28 2
EcoO109I rG'GnC_Cy
Cuts at: 3806 3806
Size: 7189
EcoRI G'AATT_C
Cuts at: 4498 4876 4498
Size: 378 6811
EcoRV GAT'ATC
Cuts at: 4003 4003
Size: 7189
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2651 2651
Size: 7189
FspI TGC'GCA
Cuts at: 5695 5695
Size: 7189
HaeII r_GCGC'y
Cuts at: 1678 6336 1678
Size: 4658 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2115 2147 2826 2858 3005
Size: 32 135 32 543 32 679 32 147
Cuts at: 3005 3037 3388 3420 5830 5862 5904 5936 1373
Size: 32 351 32 2410 32 42 32 2626
Fragments arranged by size:
2626 2410 679 543 351 147 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2494 2633 4533 4779 669
Size: 988 281 556 139 1900 246 3079
Fragments arranged by size:
3079 1900 988 556 281 246 139
HindIII A'AGCT_T
Cuts at: 748 2813 4040 748
Size: 2065 1227 3897
Fragments arranged by size:
3897 2065 1227
HpaI GTT'AAC
Cuts at: 2494 4779 2494
Size: 2285 4904
KpnI G_GTAC'C
Cuts at: 4514 4514
Size: 7189
MfeI C'AATT_G
Cuts at: 2430 4788 2430
Size: 2358 4831
MluI A'CGCG_T
Cuts at: 7065 7065
Size: 7189
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2156 2173 3040 6303 6487 1305
Size: 851 17 867 3263 184 2007
Fragments arranged by size:
3263 2007 867 851 184 17
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5367
Fragments arranged by size:
5367 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2094 2387 3096 5147 5506 5665 518
Size: 648 928 293 709 2051 359 159 2042
Fragments arranged by size:
2051 2042 928 709 648 359 293 159
MspA1I CmG'CkG
Cuts at: 1606 2310 3619 3847 4605 5282 6430 6675 1606
Size: 704 1309 228 758 677 1148 245 2120
Fragments arranged by size:
2120 1309 1148 758 704 677 245 228
NcoI C'CATG_G
Cuts at: 513 1065 2004 2893 513
Size: 552 939 889 4809
Fragments arranged by size:
4809 939 889 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7189
NheI G'CTAG_C
Cuts at: 1052 3431 1052
Size: 2379 4810
NotI GC'GGCC_GC
Cuts at: 4636 4636
Size: 7189
NsiI A_TGCA'T
Cuts at: 3609 3609
Size: 7189
NspI r_CATG'y
Cuts at: 1132 1229 2377 3611 4547 6092 1132
Size: 97 1148 1234 936 1545 2229
Fragments arranged by size:
2229 1545 1234 1148 936 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7189
PciI A'CATG_T
Cuts at: 6088 6088
Size: 7189
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2192 1108
Size: 1084 6105
PfoI T'CCnGG_A
Cuts at: 2943 2943
Size: 7189
PmeI GTTT'AAAC
Cuts at: 4493 4493
Size: 7189
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5258 5290 6771 6803 5258
Size: 32 1481 32 5644
Fragments arranged by size:
5644 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3806 3806
Size: 7189
PshAI GACnn'nnGTC
Cuts at: 1613 2998 1613
Size: 1385 5804
PsiI TTA'TAA
Cuts at: 4759 4759
Size: 7189
PstI C_TGCA'G
Cuts at: 830 4541 830
Size: 3711 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7189
PvuII CAG'CTG
Cuts at: 3619 3847 3619
Size: 228 6961
SacII CC_GC'GG
Cuts at: 2311 2311
Size: 7189
SalI G'TCGA_C
Cuts at: 1936 4531 1936
Size: 2595 4594
SanDI GG'GwC_CC
Cuts at: 3806 3806
Size: 7189
SbfI CC_TGCA'GG
Cuts at: 4541 4541
Size: 7189
ScaI AGT'ACT
Cuts at: 5437 7149 5437
Size: 1712 5477
SfcI C'TryA_G
Cuts at: 826 1045 2878 3484 3718 4198 4537 5672 6353
Size: 219 1833 606 234 480 339 1135 681
Cuts at: 6353 6544 826
Size: 191 1471
Fragments arranged by size:
1833 1471 1135 681 606 480 339 234
219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7189
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7189
SmaI CCC'GGG
Cuts at: 1872 4516 1872
Size: 2644 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2636 5293 6194 6456 820
Size: 197 926 693 2657 901 262 1553
Fragments arranged by size:
2657 1553 926 901 693 262 197
SnaBI TAC'GTA
Cuts at: 493 4980 493
Size: 4487 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7189
SphI G_CATG'C
Cuts at: 1132 3611 4547 1132
Size: 2479 936 3774
Fragments arranged by size:
3774 2479 936
SspI AAT'ATT
Cuts at: 5 52 3230 5113 5
Size: 47 3178 1883 2081
Fragments arranged by size:
3178 2081 1883 47
StuI AGG'CCT
Cuts at: 1517 4196 1517
Size: 2679 4510
StyI C'CwwG_G
Cuts at: 513 1065 2004 2026 2893 4137 4177 4628 513
Size: 552 939 22 867 1244 40 451 3074
Fragments arranged by size:
3074 1244 939 867 552 451 40 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2562 2566 3659 5216 5233 5386 5571
Size: 1255 982 4 1093 1557 17 153 185
Cuts at: 5571 6016 325
Size: 445 1498
Fragments arranged by size:
1557 1498 1255 1093 982 445 185 153
17 4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2419 5435 7147
Size: 274 80 33 51 1443 442 3016 1712
Cuts at: 7147 96
Size: 138
Fragments arranged by size:
3016 1712 1443 442 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7149 7181 7149
Size: 32 7157
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7189
XbaI T'CTAG_A
Cuts at: 4459 4525 4459
Size: 66 7123
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3463 3619 3463
Size: 156 7033
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7189
XmaI C'CCGG_G
Cuts at: 1870 4514 1870
Size: 2644 4545
XmnI GAAnn'nnTTC
Cuts at: 2913 4467 5318 2913
Size: 1554 851 4784
Fragments arranged by size:
4784 1554 851
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4997 276
Size: 53 83 186 936 3463 2468
Fragments arranged by size:
3463 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoNI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI
PspXI PsrI RsrII SacI SapI SexAI SfiI SfoI
SrfI SwaI