Restriction map for
pFN21AA1558
(Circular) (Six-base) MAPSORT of: pFN21AA1558.seq Check: 3575 from: 1 to: 7342
pFN21AA1558 7342 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3671 5152 278
Size: 53 83 186 936 2135 1481 2468
Fragments arranged by size:
2468 2135 1481 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2454 3149 4490 4685 1937
Size: 517 695 1341 195 4594
Fragments arranged by size:
4594 1341 695 517 195
Acc65I G'GTAC_C
Cuts at: 4663 4663
Size: 7342
AclI AA'CG_TT
Cuts at: 1563 5469 5842 1563
Size: 3906 373 3063
Fragments arranged by size:
3906 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2096 2359 3978 4608 5405 6789 7057 1632
Size: 464 263 1619 630 797 1384 268 1917
Fragments arranged by size:
1917 1619 1384 797 630 464 268 263
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7145
AflIII A'CryG_T
Cuts at: 1203 2516 3135 6241 7218 1203
Size: 1313 619 3106 977 1327
Fragments arranged by size:
3106 1327 1313 977 619
AgeI A'CCGG_T
Cuts at: 6173 6173
Size: 7342
AhdI GACnn_n'nnGTC
Cuts at: 6071 6071
Size: 7342
AleI CACnn'nnGTG
Cuts at: 1166 4625 1166
Size: 3459 3883
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7342
AlwNI CAG_nnn'CTG
Cuts at: 3956 4439 5085 6657 3956
Size: 483 646 1572 4641
Fragments arranged by size:
4641 1572 646 483
ApaI G_GGCC'C
Cuts at: 3431 3431
Size: 7342
ApaLI G'TGCA_C
Cuts at: 3410 5399 6555 3410
Size: 1989 1156 4197
Fragments arranged by size:
4197 1989 1156
ApoI r'AATT_y
Cuts at: 1493 2015 2688 3183 4651 4877 5029 7138 1493
Size: 522 673 495 1468 226 152 2109 1697
Fragments arranged by size:
2109 1697 1468 673 522 495 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3302 5896 160
Size: 872 2270 2594 1606
Fragments arranged by size:
2594 2270 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7342
AvaI C'yCGr_G
Cuts at: 1870 1943 3922 4532 4583 4667 1870
Size: 73 1979 610 51 84 4545
Fragments arranged by size:
4545 1979 610 84 73 51
BamHI G'GATC_C
Cuts at: 3013 4672 5039 3013
Size: 1659 367 5316
Fragments arranged by size:
5316 1659 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4558 4663 6118 618
Size: 325 217 627 2771 105 1455 1842
Fragments arranged by size:
2771 1842 1455 627 325 217 105
BanII G_rGCy'C
Cuts at: 1391 2709 3431 4661 1391
Size: 1318 722 1230 4072
Fragments arranged by size:
4072 1318 1230 722
BbeI G_GCGC'C
Cuts at: 4562 4562
Size: 7342
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2944 2999 3367 4179 4438 928
Size: 971 1045 55 368 812 259 3832
Fragments arranged by size:
3832 1045 971 812 368 259 55
BbvCI CC'TCA_GC
Cuts at: 3351 3351
Size: 7342
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5533 5567 1903
Size: 34 3596 34 3678
Fragments arranged by size:
3678 3596 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2244 5234 6444 2244
Size: 2990 1210 3142
Fragments arranged by size:
3142 2990 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7342
BfrBI ATG'CAT
Cuts at: 2636 2636
Size: 7342
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2880 5953 136
Size: 107 122 71 631 605 1208 3073 1525
Fragments arranged by size:
3073 1525 1208 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2868 3463 3544 7337 2868
Size: 595 81 3793 2873
Fragments arranged by size:
3793 2873 595 81
BlpI GC'TnA_GC
Cuts at: 4409 4759 4409
Size: 350 6992
Bme1580I G_kGCm'C
Cuts at: 1790 3414 3431 5403 6559 1790
Size: 1624 17 1972 1156 2573
Fragments arranged by size:
2573 1972 1624 1156 17
BmgBI CAC'GTC
Cuts at: 1318 2517 4185 1318
Size: 1199 1668 4475
Fragments arranged by size:
4475 1668 1199
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2300 4266 5073
Size: 416 53 83 399 24 883 1966 807
Cuts at: 5073 6031 442
Size: 958 1753
Fragments arranged by size:
1966 1753 958 883 807 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7342
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4239 4239
Size: 7342
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2987 4144 4209 6002 1239
Size: 1748 1157 65 1793 2579
Fragments arranged by size:
2579 1793 1748 1157 65
Bpu10I CC'TnA_GC
Cuts at: 2504 2666 3351 4193 2504
Size: 162 685 842 5653
Fragments arranged by size:
5653 842 685 162
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2187 2852 4482 5467 6332 6630 2187
Size: 665 1630 985 865 298 2899
Fragments arranged by size:
2899 1630 985 865 665 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2667 6005 882
Size: 617 1168 3338 2219
Fragments arranged by size:
3338 2219 1168 617
BsaAI yAC'GTr
Cuts at: 493 1204 5133 493
Size: 711 3929 2702
Fragments arranged by size:
3929 2702 711
BsaBI GATnn'nnATC
Cuts at: 7336 7336
Size: 7342
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3668 4559 5149 5531
Size: 53 83 186 936 2135 891 590 382
Cuts at: 5531 6166 275
Size: 635 1451
Fragments arranged by size:
2135 1451 936 891 635 590 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5775 6173 6447 6594 7005 5775
Size: 398 274 147 411 6112
Fragments arranged by size:
6112 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7117
Fragments arranged by size:
7117 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2582 4148 4358 4538 1186
Size: 1396 1566 210 180 3990
Fragments arranged by size:
3990 1566 1396 210 180
BseYI C'CCAG_C
Cuts at: 3995 4161 4571 6545 7041 3995
Size: 166 410 1974 496 4296
Fragments arranged by size:
4296 1974 496 410 166
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3467 3487 4313 1163
Size: 573 1731 20 826 4192
Fragments arranged by size:
4192 1731 826 573 20
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4792 5553 6581 1540
Size: 275 186 2791 761 1028 2301
Fragments arranged by size:
2791 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3414 4096 4130 4199 5403 5488 6559 3414
Size: 682 34 69 1204 85 1071 4197
Fragments arranged by size:
4197 1204 1071 682 85 69 34
BsmI GAATG_Cn'
Cuts at: 3890 4853 4946 3890
Size: 963 93 6286
Fragments arranged by size:
6286 963 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3488 4148 3488
Size: 660 6682
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2709 3414 3431 4096 4130 4199 4661
Size: 399 919 705 17 665 34 69 462
Cuts at: 4661 5403 5488 6559 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 919 742 705 665 462 399
85 69 34 17
BspEI T'CCGG_A
Cuts at: 7005 7005
Size: 7342
BspHI T'CATG_A
Cuts at: 2953 5231 6961 2953
Size: 2278 1730 3334
Fragments arranged by size:
3334 2278 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3566 4697 844
Size: 2722 1131 3489
Fragments arranged by size:
3489 2722 1131
BsrBI CCG'CTC
Cuts at: 1394 1823 5229 1394
Size: 429 3406 3507
Fragments arranged by size:
3507 3406 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3739 5837 6011 65
Size: 1183 2491 2098 174 1396
Fragments arranged by size:
2491 2098 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5986 6173 1700
Size: 110 4176 187 2869
Fragments arranged by size:
4176 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7342
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7342
BssSI C'ACGA_G
Cuts at: 5402 6414 5402
Size: 1012 6330
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2548 794
Size: 1754 5588
BstBI TT'CG_AA
Cuts at: 6146 6146
Size: 7342
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7342
BstXI CCAn_nnnn'nTGG
Cuts at: 4050 4050
Size: 7342
BstYI r'GATC_y
Cuts at: 1972 2868 2945 3013 3463 3544 4112 4672 5039
Size: 896 77 68 450 81 568 560 367
Cuts at: 5039 5424 5441 6893 6979 6991 7337 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 896 568 560 450 385 367
346 86 81 77 68 17 12
BstZ17I GTA'TAC
Cuts at: 3150 3150
Size: 7342
Bsu36I CC'TnA_GG
Cuts at: 3514 3514
Size: 7342
BtgI C'CryG_G
Cuts at: 513 1065 4087 4254 4320 513
Size: 552 3022 167 66 3535
Fragments arranged by size:
3535 3022 552 167 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2592 3871 487
Size: 683 56 1366 1279 3958
Fragments arranged by size:
3958 1366 1279 683 56
BtsI GCAGTG_nn'
Cuts at: 4006 4296 4318 4858 5652 5672 4006
Size: 290 22 540 794 20 5676
Fragments arranged by size:
5676 794 540 290 22 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7342
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7307
DraI TTT'AAA
Cuts at: 3598 4646 3598
Size: 1048 6294
DraIII CAC_nnn'GTG
Cuts at: 2651 4616 2651
Size: 1965 5377
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6349 809
Size: 507 5033 1802
Fragments arranged by size:
5033 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4317 4597 4789
Size: 54 1425 50 275 18 2487 280 192
Cuts at: 4789 5678 7307 8
Size: 889 1629 43
Fragments arranged by size:
2487 1629 1425 889 280 275 192 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4789 1537
Size: 275 2977 4090
Fragments arranged by size:
4090 2977 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5272 1353
Size: 3919 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5929 6303 6449 387
Size: 1064 138 4340 374 146 1280
Fragments arranged by size:
4340 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2096 2359 2987 3978 4144 4209 4608
Size: 393 464 263 628 991 166 65 399
Cuts at: 4608 5405 6002 6789 7057 1239
Size: 797 597 787 268 1524
Fragments arranged by size:
1524 991 797 787 628 597 464 399
393 268 263 166 65
EcoO109I rG'GnC_Cy
Cuts at: 3097 3427 3428 3097
Size: 330 1 7011
Fragments arranged by size:
7011 330 1
EcoRI G'AATT_C
Cuts at: 4651 5029 4651
Size: 378 6964
EcoRV GAT'ATC
Cuts at: 3057 3057
Size: 7342
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4000 4000
Size: 7342
FspI TGC'GCA
Cuts at: 5848 5848
Size: 7342
HaeII r_GCGC'y
Cuts at: 1678 4562 6489 1678
Size: 2884 1927 2531
Fragments arranged by size:
2884 2531 1927
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2012 2044 2162 2194 3236
Size: 32 135 32 440 32 118 32 1042
Cuts at: 3236 3268 3737 3769 3836 3868 4104 4136 4169
Size: 32 469 32 67 32 236 32 33
Cuts at: 4169 4201 5983 6015 6057 6089 1373
Size: 32 1782 32 42 32 2626
Fragments arranged by size:
2626 1782 1042 469 440 236 135 118
67 42 33 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4686 4932 669
Size: 988 281 2748 246 3079
Fragments arranged by size:
3079 2748 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7342
HpaI GTT'AAC
Cuts at: 4932 4932
Size: 7342
KasI G'GCGC_C
Cuts at: 4558 4558
Size: 7342
KpnI G_GTAC'C
Cuts at: 4667 4667
Size: 7342
MfeI C'AATT_G
Cuts at: 4941 4941
Size: 7342
MluI A'CGCG_T
Cuts at: 7218 7218
Size: 7342
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6456 6640 1305
Size: 5151 184 2007
Fragments arranged by size:
5151 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4319 4599 10
Size: 54 1425 343 2487 280 2753
Fragments arranged by size:
2753 2487 1425 343 280 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2631 4625 5300 5659 5818 518
Size: 648 1465 1994 675 359 159 2042
Fragments arranged by size:
2042 1994 1465 675 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4758 5435 6583 6828 1606
Size: 3152 677 1148 245 2120
Fragments arranged by size:
3152 2120 1148 677 245
NarI GG'CG_CC
Cuts at: 4559 4559
Size: 7342
NcoI C'CATG_G
Cuts at: 513 1065 4254 4320 513
Size: 552 3189 66 3535
Fragments arranged by size:
3535 3189 552 66
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7342
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7342
NotI GC'GGCC_GC
Cuts at: 4789 4789
Size: 7342
NsiI A_TGCA'T
Cuts at: 2638 2638
Size: 7342
NspI r_CATG'y
Cuts at: 1132 1229 3139 3816 4700 6245 1132
Size: 97 1910 677 884 1545 2229
Fragments arranged by size:
2229 1910 1545 884 677 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7342
PciI A'CATG_T
Cuts at: 3135 6241 3135
Size: 3106 4236
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 6440
PmeI GTTT'AAAC
Cuts at: 4646 4646
Size: 7342
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5411 5443 6924 6956 5411
Size: 32 1481 32 5797
Fragments arranged by size:
5797 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3097 3097
Size: 7342
PshAI GACnn'nnGTC
Cuts at: 1613 4442 1613
Size: 2829 4513
PsiI TTA'TAA
Cuts at: 2465 4912 2465
Size: 2447 4895
PspOMI G'GGCC_C
Cuts at: 3427 3427
Size: 7342
PstI C_TGCA'G
Cuts at: 830 4543 4694 830
Size: 3713 151 3478
Fragments arranged by size:
3713 3478 151
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7342
SalI G'TCGA_C
Cuts at: 1936 4684 1936
Size: 2748 4594
SbfI CC_TGCA'GG
Cuts at: 4694 4694
Size: 7342
ScaI AGT'ACT
Cuts at: 5590 7302 5590
Size: 1712 5630
SexAI A'CCwGG_T
Cuts at: 4612 4612
Size: 7342
SfcI C'TryA_G
Cuts at: 826 1045 4419 4539 4638 4690 5825 6506 6697
Size: 219 3374 120 99 52 1135 681 191
Cuts at: 6697 826
Size: 1471
Fragments arranged by size:
3374 1471 1135 681 219 191 120 99
52
SfoI GGC'GCC
Cuts at: 4560 4560
Size: 7342
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7342
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7342
SmaI CCC'GGG
Cuts at: 1872 4669 1872
Size: 2797 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2202 2831 4497 5446 6347 6609
Size: 197 926 259 629 1666 949 901 262
Cuts at: 6609 820
Size: 1553
Fragments arranged by size:
1666 1553 949 926 901 629 262 259
197
SnaBI TAC'GTA
Cuts at: 493 5133 493
Size: 4640 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7342
SphI G_CATG'C
Cuts at: 1132 4700 1132
Size: 3568 3774
SspI AAT'ATT
Cuts at: 5 52 2767 3796 5266 5
Size: 47 2715 1029 1470 2081
Fragments arranged by size:
2715 2081 1470 1029 47
StuI AGG'CCT
Cuts at: 1517 4567 1517
Size: 3050 4292
StyI C'CwwG_G
Cuts at: 513 1065 4254 4320 4781 513
Size: 552 3189 66 461 3074
Fragments arranged by size:
3189 3074 552 461 66
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2989 4060 5369 5386 5539 5724 6169
Size: 1255 1409 1071 1309 17 153 185 445
Cuts at: 6169 325
Size: 1498
Fragments arranged by size:
1498 1409 1309 1255 1071 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2699 3929 3947
Size: 274 80 33 51 1443 722 1230 18
Cuts at: 3947 4358 5588 7300 96
Size: 411 1230 1712 138
Fragments arranged by size:
1712 1443 1230 1230 722 411 274 138
80 51 33 18
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2018 2050 7302 7334 2018
Size: 32 5252 32 2026
Fragments arranged by size:
5252 2026 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3476 1316
Size: 2160 5182
XbaI T'CTAG_A
Cuts at: 2053 2728 2920 4678 2053
Size: 675 192 1758 4717
Fragments arranged by size:
4717 1758 675 192
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7342
XmaI C'CCGG_G
Cuts at: 1870 4667 1870
Size: 2797 4545
XmnI GAAnn'nnTTC
Cuts at: 2598 2658 3137 5471 2598
Size: 60 479 2334 4469
Fragments arranged by size:
4469 2334 479 60
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3669 5150 276
Size: 53 83 186 936 2135 1481 2468
Fragments arranged by size:
2468 2135 1481 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BsiWI EcoICRI
EcoNI FseI FspAI NaeI NgoMIV NruI PacI PfoI
PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SfiI SrfI SwaI XcmI