Restriction map for
pFN21AA1555
(Circular) (Six-base) MAPSORT of: pFN21AA1555.seq Check: 1123 from: 1 to: 11920
pFN21AA1555 11920 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3373 4195 4510 8547 8905 3373
Size: 822 315 4037 358 6388
Fragments arranged by size:
6388 4037 822 358 315
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7341 9730 278
Size: 53 83 186 936 5805 2389 2468
Fragments arranged by size:
5805 2468 2389 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 9263 1937
Size: 7326 4594
Acc65I G'GTAC_C
Cuts at: 8174 9241 8174
Size: 1067 10853
AclI AA'CG_TT
Cuts at: 1563 4998 10047 10420 1563
Size: 3435 5049 373 3063
Fragments arranged by size:
5049 3435 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2877 3087 3785 3995 5638 5732 7331 8081
Size: 1245 210 698 210 1643 94 1599 750
Cuts at: 8081 8515 9983 11367 11635 1632
Size: 434 1468 1384 268 1917
Fragments arranged by size:
1917 1643 1599 1468 1384 1245 750 698
434 268 210 210 94
AflII C'TTAA_G
Cuts at: 820 1017 3106 820
Size: 197 2089 9634
Fragments arranged by size:
9634 2089 197
AflIII A'CryG_T
Cuts at: 1203 3738 4926 6292 8152 10819 11796 1203
Size: 2535 1188 1366 1860 2667 977 1327
Fragments arranged by size:
2667 2535 1860 1366 1327 1188 977
AgeI A'CCGG_T
Cuts at: 10751 10751
Size: 11920
AhdI GACnn_n'nnGTC
Cuts at: 10649 10649
Size: 11920
AleI CACnn'nnGTG
Cuts at: 1166 2649 5945 1166
Size: 1483 3296 7141
Fragments arranged by size:
7141 3296 1483
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 11920
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2941 2973 2941
Size: 32 11888
AlwNI CAG_nnn'CTG
Cuts at: 2855 3094 4058 5174 6059 7526 7788 8925 9663
Size: 239 964 1116 885 1467 262 1137 738
Cuts at: 9663 11235 2855
Size: 1572 3540
Fragments arranged by size:
3540 1572 1467 1137 1116 964 885 738
262 239
ApaI G_GGCC'C
Cuts at: 2189 5315 5343 6138 7703 8072 8652 8707 2189
Size: 3126 28 795 1565 369 580 55 5402
Fragments arranged by size:
5402 3126 1565 795 580 369 55 28
ApaLI G'TGCA_C
Cuts at: 9009 9977 11133 9009
Size: 968 1156 9796
Fragments arranged by size:
9796 1156 968
ApoI r'AATT_y
Cuts at: 1493 4392 5486 6537 7284 8786 9229 9455 9607
Size: 2899 1094 1051 747 1502 443 226 152
Cuts at: 9607 11716 1493
Size: 2109 1697
Fragments arranged by size:
2899 2109 1697 1502 1094 1051 747 443
226 152
AseI AT'TA_AT
Cuts at: 160 1032 10474 160
Size: 872 9442 1606
Fragments arranged by size:
9442 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11920
AvaI C'yCGr_G
Cuts at: 1870 1943 2182 2465 4223 4439 4798 4959 5299
Size: 73 239 283 1758 216 359 161 340
Cuts at: 5299 8004 8145 8168 8706 8854 9023 9245 1870
Size: 2705 141 23 538 148 169 222 4545
Fragments arranged by size:
4545 2705 1758 538 359 340 283 239
222 216 169 161 148 141 73 23
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 6770 6803 6770
Size: 33 11887
BamHI G'GATC_C
Cuts at: 2008 2805 8598 9250 9617 2008
Size: 797 5793 652 367 4311
Fragments arranged by size:
5793 4311 797 652 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2030 3358 4037 4919 6700
Size: 325 217 627 243 1328 679 882 1781
Cuts at: 6700 7645 8174 8893 9241 10696 618
Size: 945 529 719 348 1455 1842
Fragments arranged by size:
1842 1781 1455 1328 945 882 719 679
627 529 348 325 243 217
BanII G_rGCy'C
Cuts at: 1391 2150 2163 2189 2418 2832 2898 3152 3473
Size: 759 13 26 229 414 66 254 321
Cuts at: 3473 3528 4994 5315 5343 6138 6713 7703 7850
Size: 55 1466 321 28 795 575 990 147
Cuts at: 7850 8072 8629 8652 8707 8750 8907 8990 9174
Size: 222 557 23 55 43 157 83 184
Cuts at: 9174 9239 1391
Size: 65 4072
Fragments arranged by size:
4072 1466 990 795 759 575 557 414
321 321 254 229 222 184 157 147
83 66 65 55 55 43 28 26
23 13
BbeI G_GCGC'C
Cuts at: 7649 7649
Size: 11920
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2485 3018 3075 3348 4473 4649 5206
Size: 971 586 533 57 273 1125 176 557
Cuts at: 5206 5700 7593 7909 928
Size: 494 1893 316 4939
Fragments arranged by size:
4939 1893 1125 971 586 557 533 494
316 273 176 57
BbvCI CC'TCA_GC
Cuts at: 2043 5698 5893 7704 8020 8296 8375 2043
Size: 3655 195 1811 316 276 79 5588
Fragments arranged by size:
5588 3655 1811 316 276 195 79
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2301 2335 6697 6731 9014 9048 10111
Size: 34 364 34 4362 34 2283 34 1063
Cuts at: 10111 10145 1903
Size: 34 3678
Fragments arranged by size:
4362 3678 2283 1063 364 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 9812 11022 9812
Size: 1210 10710
BclI T'GATC_A
Cuts at: 1548 1548
Size: 11920
BfrBI ATG'CAT
Cuts at: 4102 4102
Size: 11920
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2679 4600 5090
Size: 107 122 71 631 605 1007 1921 490
Cuts at: 5090 5807 8037 8711 10531 136
Size: 717 2230 674 1820 1525
Fragments arranged by size:
2230 1921 1820 1525 1007 717 674 631
605 490 122 107 71
BglII A'GATC_T
Cuts at: 6024 7105 11915 6024
Size: 1081 4810 6029
Fragments arranged by size:
6029 4810 1081
BlpI GC'TnA_GC
Cuts at: 3572 4840 6052 7767 7870 9337 3572
Size: 1268 1212 1715 103 1467 6155
Fragments arranged by size:
6155 1715 1467 1268 1212 103
Bme1580I G_kGCm'C
Cuts at: 1790 2033 2189 4299 4922 5315 5343 5352 6138
Size: 243 156 2110 623 393 28 9 786
Cuts at: 6138 7703 8072 8572 8652 8707 8896 9013 9181
Size: 1565 369 500 80 55 189 117 168
Cuts at: 9181 9981 11137 1790
Size: 800 1156 2573
Fragments arranged by size:
2573 2110 1565 1156 800 786 623 500
393 369 243 189 168 156 117 80
55 28 9
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 11920
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2980 2995 3585
Size: 416 53 83 399 24 1563 15 590
Cuts at: 3585 4121 4477 4878 5081 5731 6031 9651 10609
Size: 536 356 401 203 650 300 3620 958
Cuts at: 10609 442
Size: 1753
Fragments arranged by size:
3620 1753 1563 958 650 590 536 416
401 399 356 300 203 83 53 24
15
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 11920
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5181 8238 5181
Size: 3057 8863
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2366 2499 2609 2813 2911 3563 4035 4277
Size: 1127 133 110 204 98 652 472 242
Cuts at: 4277 5064 5151 5466 5567 6278 6721 7472 7574
Size: 787 87 315 101 711 443 751 102
Cuts at: 7574 8208 8752 8828 9004 10580 1239
Size: 634 544 76 176 1576 2579
Fragments arranged by size:
2579 1576 1127 787 751 711 652 634
544 472 443 315 242 204 176 133
110 102 101 98 87 76
Bpu10I CC'TnA_GC
Cuts at: 2043 2334 3053 3722 5698 5893 7704 8020 8296
Size: 291 719 669 1976 195 1811 316 276
Cuts at: 8296 8375 8775 2043
Size: 79 400 5188
Fragments arranged by size:
5188 1976 1811 719 669 400 316 291
276 195 79
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6271 6410 7897 10045 10910 11208 6271
Size: 139 1487 2148 865 298 6983
Fragments arranged by size:
6983 2148 1487 865 298 139
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2374 2521 7762 8091 8226 8384 8785
Size: 617 875 147 5241 329 135 158 401
Cuts at: 8785 10583 882
Size: 1798 2219
Fragments arranged by size:
5241 2219 1798 875 617 401 329 158
147 135
BsaAI yAC'GTr
Cuts at: 493 1204 3739 8047 9711 493
Size: 711 2535 4308 1664 2702
Fragments arranged by size:
4308 2702 2535 1664 711
BsaBI GATnn'nnATC
Cuts at: 7196 8588 11914 7196
Size: 1392 3326 7202
Fragments arranged by size:
7202 3326 1392
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 7338 7646 8965 9727
Size: 53 83 186 936 5805 308 1319 762
Cuts at: 9727 10109 10744 275
Size: 382 635 1451
Fragments arranged by size:
5805 1451 1319 936 762 635 382 308
186 83 53
BsaWI w'CCGG_w
Cuts at: 4349 10353 10751 11025 11172 11583 4349
Size: 6004 398 274 147 411 4686
Fragments arranged by size:
6004 4686 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2941 2971 3515 3545 3830
Size: 30 165 30 1538 30 544 30 285
Cuts at: 3830 3860 4400 4430 5601 5631 5678 5708 5736
Size: 30 540 30 1171 30 47 30 28
Cuts at: 5736 5766 5893 5923 6672 6702 7526 7556 1178
Size: 30 127 30 749 30 824 30 5542
Fragments arranged by size:
5542 1538 1171 824 749 544 540 285
165 127 47 30 30 30 30 30
30 30 30 30 30 30 30 28
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2840 2876 3830 4162 4387 4604 4664 5137
Size: 1654 36 954 332 225 217 60 473
Cuts at: 5137 5686 5881 5901 6374 6377 7150 7166 7289
Size: 549 195 20 473 3 773 16 123
Cuts at: 7289 7469 7547 7619 7622 7675 8097 8363 8452
Size: 180 78 72 3 53 422 266 89
Cuts at: 8452 8534 8788 1186
Size: 82 254 4318
Fragments arranged by size:
4318 1654 954 773 549 473 473 422
332 266 254 225 217 195 180 123
89 82 78 72 60 53 36 20
16 3 3
BseYI C'CCAG_C
Cuts at: 2615 3315 3350 4665 4686 4693 4940 5622 5638
Size: 700 35 1315 21 7 247 682 16
Cuts at: 5638 5834 5881 6336 6609 6791 7301 7828 7986
Size: 196 47 455 273 182 510 527 158
Cuts at: 7986 8279 8553 9097 11123 11619 2615
Size: 293 274 544 2026 496 2916
Fragments arranged by size:
2916 2026 1315 700 682 544 527 510
496 455 293 274 273 247 196 182
158 47 35 21 16 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3222 3631 5480 5582 6135 8027 8531
Size: 573 1486 409 1849 102 553 1892 504
Cuts at: 8531 8630 8911 1163
Size: 99 281 4172
Fragments arranged by size:
4172 1892 1849 1486 573 553 504 409
281 102 99
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4604 7255 8954 9370 10131 11159
Size: 275 186 2603 2651 1699 416 761 1028
Cuts at: 11159 1540
Size: 2301
Fragments arranged by size:
2651 2603 2301 1699 1028 761 416 275
186
BsiHKAI G_wGCw'C
Cuts at: 2163 2625 2898 3048 3396 3473 3955 6713 7539
Size: 462 273 150 348 77 482 2758 826
Cuts at: 7539 8152 8160 8750 9013 9981 10066 11137 2163
Size: 613 8 590 263 968 85 1071 2946
Fragments arranged by size:
2946 2758 1071 968 826 613 590 482
462 348 273 263 150 85 77 8
BsmI GAATG_Cn'
Cuts at: 2312 9431 9524 2312
Size: 7119 93 4708
Fragments arranged by size:
7119 4708 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2033 2150 2163 2189 2418 2625 2832
Size: 399 243 117 13 26 229 207 207
Cuts at: 2832 2898 3048 3152 3396 3473 3528 3955 4299
Size: 66 150 104 244 77 55 427 344
Cuts at: 4299 4922 4994 5315 5343 5352 6138 6713 7539
Size: 623 72 321 28 9 786 575 826
Cuts at: 7539 7703 7850 8072 8152 8160 8572 8629 8652
Size: 164 147 222 80 8 412 57 23
Cuts at: 8652 8707 8750 8896 8907 8990 9013 9174 9181
Size: 55 43 146 11 83 23 161 7
Cuts at: 9181 9239 9981 10066 11137 1391
Size: 58 742 85 1071 2174
Fragments arranged by size:
2174 1071 826 786 742 623 575 427
412 399 344 321 244 243 229 222
207 207 164 161 150 147 146 117
104 85 83 80 77 72 66 58
57 55 55 43 28 26 23 23
13 11 9 8 7
BspEI T'CCGG_A
Cuts at: 11583 11583
Size: 11920
BspHI T'CATG_A
Cuts at: 9809 11539 9809
Size: 1730 10190
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3202 3373 4195 4510 5427 8402 8547 8905
Size: 2358 171 822 315 917 2975 145 358
Cuts at: 8905 9275 844
Size: 370 3489
Fragments arranged by size:
3489 2975 2358 917 822 370 358 315
171 145
BsrBI CCG'CTC
Cuts at: 1394 1823 2803 4839 9807 1394
Size: 429 980 2036 4968 3507
Fragments arranged by size:
4968 3507 2036 980 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4819 6009 10415 10589 65
Size: 1183 3571 1190 4406 174 1396
Fragments arranged by size:
4406 3571 1396 1190 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2598 3775 4599 7183 7834 8843 9013
Size: 110 788 1177 824 2584 651 1009 170
Cuts at: 9013 10564 10751 1700
Size: 1551 187 2869
Fragments arranged by size:
2869 2584 1551 1177 1009 824 788 651
187 170 110
BsrGI T'GTAC_A
Cuts at: 96 6006 6906 96
Size: 5910 900 5110
Fragments arranged by size:
5910 5110 900
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 11920
BssSI C'ACGA_G
Cuts at: 5456 7743 9980 10992 5456
Size: 2287 2237 1012 6384
Fragments arranged by size:
6384 2287 2237 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5458 7431 794
Size: 4664 1973 5283
Fragments arranged by size:
5283 4664 1973
BstBI TT'CG_AA
Cuts at: 7203 10724 7203
Size: 3521 8399
BstEII G'GTnAC_C
Cuts at: 1184 2882 6031 1184
Size: 1698 3149 7073
Fragments arranged by size:
7073 3149 1698
BstXI CCAn_nnnn'nTGG
Cuts at: 4915 6617 8268 4915
Size: 1702 1651 8567
Fragments arranged by size:
8567 1702 1651
BstYI r'GATC_y
Cuts at: 1972 2008 2805 4575 6024 6517 7105 8598 9250
Size: 36 797 1770 1449 493 588 1493 652
Cuts at: 9250 9617 10002 10019 11471 11557 11569 11915 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1770 1493 1452 1449 797 652 588
493 385 367 346 86 36 17 12
Bsu36I CC'TnA_GG
Cuts at: 2758 4629 6956 7014 9157 9195 2758
Size: 1871 2327 58 2143 38 5483
Fragments arranged by size:
5483 2327 2143 1871 58 38
BtgI C'CryG_G
Cuts at: 513 1065 2004 3426 4085 4314 4864 5943 6327
Size: 552 939 1422 659 229 550 1079 384
Cuts at: 6327 6568 6993 7249 7907 8246 8333 8405 8494
Size: 241 425 256 658 339 87 72 89
Cuts at: 8494 8881 513
Size: 387 3552
Fragments arranged by size:
3552 1422 1079 939 659 658 552 550
425 387 384 339 256 241 229 89
87 72
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 8005 487
Size: 683 56 6779 4402
Fragments arranged by size:
6779 4402 683 56
BtsI GCAGTG_nn'
Cuts at: 3566 5363 5857 6415 7670 9436 10230 10250 3566
Size: 1797 494 558 1255 1766 794 20 5236
Fragments arranged by size:
5236 1797 1766 1255 794 558 494 20
ClaI AT'CG_AT
Cuts at: 1554 3129 1554
Size: 1575 10345
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 11885
DraI TTT'AAA
Cuts at: 6831 7378 9224 6831
Size: 547 1846 9527
Fragments arranged by size:
9527 1846 547
DraIII CAC_nnn'GTG
Cuts at: 5291 5291
Size: 11920
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 10927 809
Size: 507 9611 1802
Fragments arranged by size:
9611 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4601 5277 6996
Size: 54 1425 50 275 18 2771 676 1719
Cuts at: 6996 7252 8497 8618 8951 9015 9367 10256 11885
Size: 256 1245 121 333 64 352 889 1629
Cuts at: 11885 8
Size: 43
Fragments arranged by size:
2771 1719 1629 1425 1245 889 676 352
333 275 256 121 64 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4601 7252 8951 9367 1537
Size: 275 2789 2651 1699 416 4090
Fragments arranged by size:
4090 2789 2651 1699 416 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2529 2811 2853 4139 4154 4173 4715 6153
Size: 1176 282 42 1286 15 19 542 1438
Cuts at: 6153 6434 6628 7125 7221 8125 8348 9850 1353
Size: 281 194 497 96 904 223 1502 3423
Fragments arranged by size:
3423 1502 1438 1286 1176 904 542 497
282 281 223 194 96 42 19 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3481 4538 8467 8862 10507 10881
Size: 1064 138 1892 1057 3929 395 1645 374
Cuts at: 10881 11027 387
Size: 146 1280
Fragments arranged by size:
3929 1892 1645 1280 1064 1057 395 374
146 138
EcoICRI GAG'CTC
Cuts at: 2161 2896 3471 6711 8748 2161
Size: 735 575 3240 2037 5333
Fragments arranged by size:
5333 3240 2037 735 575
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2366 2499 2609 2813 2877 2911 3087
Size: 393 734 133 110 204 64 34 176
Cuts at: 3087 3563 3785 3995 4035 4277 5064 5151 5466
Size: 476 222 210 40 242 787 87 315
Cuts at: 5466 5567 5638 5732 6278 6721 7331 7472 7574
Size: 101 71 94 546 443 610 141 102
Cuts at: 7574 8081 8208 8515 8752 8828 9004 9983 10580
Size: 507 127 307 237 76 176 979 597
Cuts at: 10580 11367 11635 1239
Size: 787 268 1524
Fragments arranged by size:
1524 979 787 787 734 610 597 546
507 476 443 393 315 307 268 242
237 222 210 204 176 176 141 133
127 110 102 101 94 87 76 71
64 40 34
EcoNI CCTnn'n_nnAGG
Cuts at: 3795 7017 7493 9077 3795
Size: 3222 476 1584 6638
Fragments arranged by size:
6638 3222 1584 476
EcoO109I rG'GnC_Cy
Cuts at: 2186 2253 2470 2658 4310 4879 5069 5311 5328
Size: 67 217 188 1652 569 190 242 17
Cuts at: 5328 5339 6873 7700 8069 8648 8703 2186
Size: 11 1534 827 369 579 55 5403
Fragments arranged by size:
5403 1652 1534 827 579 569 369 242
217 190 188 67 55 17 11
EcoRI G'AATT_C
Cuts at: 5486 7284 9229 9607 5486
Size: 1798 1945 378 7799
Fragments arranged by size:
7799 1945 1798 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 6189 8290 6189
Size: 2101 9819
FspI TGC'GCA
Cuts at: 10426 10426
Size: 11920
HaeII r_GCGC'y
Cuts at: 1678 3772 7649 7774 8258 11067 1678
Size: 2094 3877 125 484 2809 2531
Fragments arranged by size:
3877 2809 2531 2094 484 125
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3515 3547 3830 3862 4073
Size: 32 135 32 1943 32 283 32 211
Cuts at: 4073 4105 6677 6709 6857 6889 7550 7556 7582
Size: 32 2572 32 148 32 661 6 26
Cuts at: 7582 7588 7610 7642 7853 7885 8735 8767 10561
Size: 6 22 32 211 32 850 32 1794
Cuts at: 10561 10593 10635 10667 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2572 1943 1794 850 661 283 211
211 148 135 42 32 32 32 32
32 32 32 32 32 32 32 32
26 22 6 6
HincII GTy'rAC
Cuts at: 669 1657 1938 2371 4932 6642 9264 9510 669
Size: 988 281 433 2561 1710 2622 246 3079
Fragments arranged by size:
3079 2622 2561 1710 988 433 281 246
HindIII A'AGCT_T
Cuts at: 748 6591 748
Size: 5843 6077
HpaI GTT'AAC
Cuts at: 9510 9510
Size: 11920
KasI G'GCGC_C
Cuts at: 7645 7645
Size: 11920
KpnI G_GTAC'C
Cuts at: 8178 9245 8178
Size: 1067 10853
MfeI C'AATT_G
Cuts at: 9519 9519
Size: 11920
MluI A'CGCG_T
Cuts at: 11796 11796
Size: 11920
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5160 11034 11218 1305
Size: 3855 5874 184 2007
Fragments arranged by size:
5874 3855 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 6998 10
Size: 54 1425 343 5166 4932
Fragments arranged by size:
5166 4932 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2649 4925 4953 5945 9878 10237 10396
Size: 648 1483 2276 28 992 3933 359 159
Cuts at: 10396 518
Size: 2042
Fragments arranged by size:
3933 2276 2042 1483 992 648 359 159
28
MspA1I CmG'CkG
Cuts at: 1606 2124 2132 2931 3286 4697 6308 7251 7788
Size: 518 8 799 355 1411 1611 943 537
Cuts at: 7788 8101 8300 8730 9046 9336 10013 11161 11406
Size: 313 199 430 316 290 677 1148 245
Cuts at: 11406 1606
Size: 2120
Fragments arranged by size:
2120 1611 1411 1148 943 799 677 537
518 430 355 316 313 290 245 199
8
NaeI GCC'GGC
Cuts at: 2600 3777 7185 2600
Size: 1177 3408 7335
Fragments arranged by size:
7335 3408 1177
NarI GG'CG_CC
Cuts at: 7646 7646
Size: 11920
NcoI C'CATG_G
Cuts at: 513 1065 2004 4864 5943 6327 6568 6993 8881
Size: 552 939 2860 1079 384 241 425 1888
Cuts at: 8881 513
Size: 3552
Fragments arranged by size:
3552 2860 1888 1079 939 552 425 384
241
NdeI CA'TA_TG
Cuts at: 387 3944 387
Size: 3557 8363
NgoMIV G'CCGG_C
Cuts at: 2598 3775 7183 2598
Size: 1177 3408 7335
Fragments arranged by size:
7335 3408 1177
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 11920
NotI GC'GGCC_GC
Cuts at: 9367 9367
Size: 11920
NsiI A_TGCA'T
Cuts at: 4104 4104
Size: 11920
NspI r_CATG'y
Cuts at: 1132 1229 4903 4930 4958 6863 7309 7670 7813
Size: 97 3674 27 28 1905 446 361 143
Cuts at: 7813 8156 9278 10823 1132
Size: 343 1122 1545 2229
Fragments arranged by size:
3674 2229 1905 1545 1122 446 361 343
143 97 28 27
PasI CC'CwG_GG
Cuts at: 1348 2411 2810 4915 5315 8125 8959 9174 1348
Size: 1063 399 2105 400 2810 834 215 4094
Fragments arranged by size:
4094 2810 2105 1063 834 400 399 215
PciI A'CATG_T
Cuts at: 4926 8152 10819 4926
Size: 3226 2667 6027
Fragments arranged by size:
6027 3226 2667
PflMI CCAn_nnn'nTGG
Cuts at: 1108 6028 8217 8506 1108
Size: 4920 2189 289 4522
Fragments arranged by size:
4920 4522 2189 289
PfoI T'CCnGG_A
Cuts at: 2343 2476 7494 8981 2343
Size: 133 5018 1487 5282
Fragments arranged by size:
5282 5018 1487 133
PmeI GTTT'AAAC
Cuts at: 9224 9224
Size: 11920
PmlI CAC'GTG
Cuts at: 3739 8047 3739
Size: 4308 7612
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2941 2973 9989 10021 11502 11534 2941
Size: 32 7016 32 1481 32 3327
Fragments arranged by size:
7016 3327 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2470 4310 6873 2470
Size: 1840 2563 7517
Fragments arranged by size:
7517 2563 1840
PshAI GACnn'nnGTC
Cuts at: 1613 7909 1613
Size: 6296 5624
PsiI TTA'TAA
Cuts at: 9490 9490
Size: 11920
PspOMI G'GGCC_C
Cuts at: 2185 5311 5339 6134 7699 8068 8648 8703 2185
Size: 3126 28 795 1565 369 580 55 5402
Fragments arranged by size:
5402 3126 1565 795 580 369 55 28
PspXI vC'TCGA_Gb
Cuts at: 8145 8168 8145
Size: 23 11897
PstI C_TGCA'G
Cuts at: 830 2597 2893 4036 4536 6786 8902 9272 830
Size: 1767 296 1143 500 2250 2116 370 3478
Fragments arranged by size:
3478 2250 2116 1767 1143 500 370 296
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 11920
PvuII CAG'CTG
Cuts at: 2132 4697 6308 7788 8101 2132
Size: 2565 1611 1480 313 5951
Fragments arranged by size:
5951 2565 1611 1480 313
RsrII CG'GwC_CG
Cuts at: 3288 3288
Size: 11920
SacI G_AGCT'C
Cuts at: 2163 2898 3473 6713 8750 2163
Size: 735 575 3240 2037 5333
Fragments arranged by size:
5333 3240 2037 735 575
SacII CC_GC'GG
Cuts at: 7252 7252
Size: 11920
SalI G'TCGA_C
Cuts at: 1936 9262 1936
Size: 7326 4594
SanDI GG'GwC_CC
Cuts at: 4310 4310
Size: 11920
SapI GCTCTTCn'nnn_
Cuts at: 4154 4173 6153 4154
Size: 19 1980 9921
Fragments arranged by size:
9921 1980 19
SbfI CC_TGCA'GG
Cuts at: 9272 9272
Size: 11920
ScaI AGT'ACT
Cuts at: 2591 3169 10168 11880 2591
Size: 578 6999 1712 2631
Fragments arranged by size:
6999 2631 1712 578
SfcI C'TryA_G
Cuts at: 826 1045 2593 2889 4032 4532 6782 8898 9268
Size: 219 1548 296 1143 500 2250 2116 370
Cuts at: 9268 10403 11084 11275 826
Size: 1135 681 191 1471
Fragments arranged by size:
2250 2116 1548 1471 1143 1135 681 500
370 296 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 8711 8711
Size: 11920
SfoI GGC'GCC
Cuts at: 7647 7647
Size: 11920
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11920
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 11920
SmaI CCC'GGG
Cuts at: 1872 2184 4225 5301 8708 8856 9247 1872
Size: 312 2041 1076 3407 148 391 4545
Fragments arranged by size:
4545 3407 2041 1076 391 312 148
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3106 4439 6250 6389 7876 8145
Size: 197 926 1163 1333 1811 139 1487 269
Cuts at: 8145 8168 10024 10925 11187 820
Size: 23 1856 901 262 1553
Fragments arranged by size:
1856 1811 1553 1487 1333 1163 926 901
269 262 197 139 23
SnaBI TAC'GTA
Cuts at: 493 9711 493
Size: 9218 2702
SpeI A'CTAG_T
Cuts at: 152 2867 152
Size: 2715 9205
SphI G_CATG'C
Cuts at: 1132 6863 7309 7813 9278 1132
Size: 5731 446 504 1465 3774
Fragments arranged by size:
5731 3774 1465 504 446
SrfI GCCC'GGGC
Cuts at: 8856 8856
Size: 11920
SspI AAT'ATT
Cuts at: 5 52 9844 5
Size: 47 9792 2081
Fragments arranged by size:
9792 2081 47
StuI AGG'CCT
Cuts at: 1517 2751 5150 5164 8018 8190 8863 9083 1517
Size: 1234 2399 14 2854 172 673 220 4354
Fragments arranged by size:
4354 2854 2399 1234 673 220 172 14
StyI C'CwwG_G
Cuts at: 513 1065 2004 3461 3873 4165 4302 4328 4864
Size: 552 939 1457 412 292 137 26 536
Cuts at: 4864 5193 5943 6043 6327 6372 6475 6568 6672
Size: 329 750 100 284 45 103 93 104
Cuts at: 6672 6696 6993 8274 8826 8881 9359 513
Size: 24 297 1281 552 55 478 3074
Fragments arranged by size:
3074 1457 1281 939 750 552 552 536
478 412 329 297 292 284 137 104
103 100 93 55 45 26 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2153 2756 3089 3329 4055 4726 4937
Size: 1255 573 603 333 240 726 671 211
Cuts at: 4937 4965 5036 5513 5616 5652 6050 7343 7705
Size: 28 71 477 103 36 398 1293 362
Cuts at: 7705 7744 8926 9947 9964 10117 10302 10747 325
Size: 39 1182 1021 17 153 185 445 1498
Fragments arranged by size:
1498 1293 1255 1182 1021 726 671 603
573 477 445 398 362 333 240 211
185 153 103 71 39 36 28 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2577 2589 3167
Size: 274 80 33 51 1443 600 12 578
Cuts at: 3167 5844 6006 6906 10166 11878 96
Size: 2677 162 900 3260 1712 138
Fragments arranged by size:
3260 2677 1712 1443 900 600 578 274
162 138 80 51 33 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2708 2740 4400 4432 5736 5768 7014 7046 11880
Size: 32 1660 32 1304 32 1246 32 4834
Cuts at: 11880 11912 2708
Size: 32 2716
Fragments arranged by size:
4834 2716 1660 1304 1246 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 11920
XbaI T'CTAG_A
Cuts at: 9256 9256
Size: 11920
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2362 5950 7905 9043 2362
Size: 3588 1955 1138 5239
Fragments arranged by size:
5239 3588 1955 1138
XhoI C'TCGA_G
Cuts at: 1943 4439 8145 8168 1943
Size: 2496 3706 23 5695
Fragments arranged by size:
5695 3706 2496 23
XmaI C'CCGG_G
Cuts at: 1870 2182 4223 5299 8706 8854 9245 1870
Size: 312 2041 1076 3407 148 391 4545
Fragments arranged by size:
4545 3407 2041 1076 391 312 148
XmnI GAAnn'nnTTC
Cuts at: 8528 10049 8528
Size: 1521 10399
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7339 9728 276
Size: 53 83 186 936 5805 2389 2468
Fragments arranged by size:
5805 2468 2389 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BsiWI BsmBI BstZ17I EcoRV FseI
FspAI NruI PacI PsrI SexAI SwaI