Restriction map for
pFN21AA1533
(Circular) (Six-base) MAPSORT of: pFN21AA1533.seq Check: 1712 from: 1 to: 6880
pFN21AA1533 6880 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4690 278
Size: 53 83 186 936 3154 2468
Fragments arranged by size:
3154 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2916 4223 1937
Size: 979 1307 4594
Fragments arranged by size:
4594 1307 979
Acc65I G'GTAC_C
Cuts at: 4201 4201
Size: 6880
AclI AA'CG_TT
Cuts at: 1563 5007 5380 1563
Size: 3444 373 3063
Fragments arranged by size:
3444 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2483 2501 4943 6327 6595 1632
Size: 851 18 2442 1384 268 1917
Fragments arranged by size:
2442 1917 1384 851 268 18
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6683
AflIII A'CryG_T
Cuts at: 1203 2474 5779 6756 1203
Size: 1271 3305 977 1327
Fragments arranged by size:
3305 1327 1271 977
AgeI A'CCGG_T
Cuts at: 5711 5711
Size: 6880
AhdI GACnn_n'nnGTC
Cuts at: 2631 5609 2631
Size: 2978 3902
AleI CACnn'nnGTG
Cuts at: 1166 2138 3233 1166
Size: 972 1095 4813
Fragments arranged by size:
4813 1095 972
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2258 1184
Size: 1074 5806
AlwNI CAG_nnn'CTG
Cuts at: 2095 3355 3737 4060 4623 6195 2095
Size: 1260 382 323 563 1572 2780
Fragments arranged by size:
2780 1572 1260 563 382 323
ApaI G_GGCC'C
Cuts at: 2569 3003 3017 3323 3723 3818 2569
Size: 434 14 306 400 95 5631
Fragments arranged by size:
5631 434 400 306 95 14
ApaLI G'TGCA_C
Cuts at: 4040 4937 6093 4040
Size: 897 1156 4827
Fragments arranged by size:
4827 1156 897
ApoI r'AATT_y
Cuts at: 1493 4189 4415 4567 6676 1493
Size: 2696 226 152 2109 1697
Fragments arranged by size:
2696 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5434 160
Size: 872 4402 1606
Fragments arranged by size:
4402 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6880
AvaI C'yCGr_G
Cuts at: 1870 1943 2788 2862 2971 3654 3750 3769 3818
Size: 73 845 74 109 683 96 19 49
Cuts at: 3818 4205 1870
Size: 387 4545
Fragments arranged by size:
4545 845 683 387 109 96 74 73
49 19
AvrII C'CTAG_G
Cuts at: 2206 2206
Size: 6880
BamHI G'GATC_C
Cuts at: 4210 4577 4210
Size: 367 6513
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2132 2179 2649 4201 5656
Size: 325 217 627 345 47 470 1552 1455
Cuts at: 5656 618
Size: 1842
Fragments arranged by size:
1842 1552 1455 627 470 345 325 217
47
BanII G_rGCy'C
Cuts at: 1391 2270 2569 2646 2985 3003 3017 3248 3323
Size: 879 299 77 339 18 14 231 75
Cuts at: 3323 3609 3709 3723 3776 3818 4080 4199 1391
Size: 286 100 14 53 42 262 119 4072
Fragments arranged by size:
4072 879 339 299 286 262 231 119
100 77 75 53 42 18 14 14
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3136 3492 3585 928
Size: 971 1237 356 93 4223
Fragments arranged by size:
4223 1237 971 356 93
BbvCI CC'TCA_GC
Cuts at: 3779 3779
Size: 6880
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5071 5105 1903
Size: 34 3134 34 3678
Fragments arranged by size:
3678 3134 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4772 5982 4772
Size: 1210 5670
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6880
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2998 5491 136
Size: 107 122 71 631 605 1326 2493 1525
Fragments arranged by size:
2493 1525 1326 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2523 6875 2523
Size: 4352 2528
BlpI GC'TnA_GC
Cuts at: 2264 3159 3242 4297 2264
Size: 895 83 1055 4847
Fragments arranged by size:
4847 1055 895 83
Bme1580I G_kGCm'C
Cuts at: 1790 2569 3003 3017 3323 3723 3751 3818 3841
Size: 779 434 14 306 400 28 67 23
Cuts at: 3841 4044 4941 6097 1790
Size: 203 897 1156 2573
Fragments arranged by size:
2573 1156 897 779 434 400 306 203
67 28 23 14
BmgBI CAC'GTC
Cuts at: 1318 3235 1318
Size: 1917 4963
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2816 3027 3087
Size: 416 53 83 399 24 1399 211 60
Cuts at: 3087 3326 3828 4611 5569 442
Size: 239 502 783 958 1753
Fragments arranged by size:
1753 1399 958 783 502 416 399 239
211 83 60 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6880
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3577 3577
Size: 6880
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2363 2923 3152 3185 3271 3617 3653 4124
Size: 1124 560 229 33 86 346 36 471
Cuts at: 4124 5540 1239
Size: 1416 2579
Fragments arranged by size:
2579 1416 1124 560 471 346 229 86
36 33
Bpu10I CC'TnA_GC
Cuts at: 2118 2771 3695 3779 2118
Size: 653 924 84 5219
Fragments arranged by size:
5219 924 653 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5005 5870 6168 5005
Size: 865 298 5717
Fragments arranged by size:
5717 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2435 5543 882
Size: 617 936 3108 2219
Fragments arranged by size:
3108 2219 936 617
BsaAI yAC'GTr
Cuts at: 493 1204 4671 493
Size: 711 3467 2702
Fragments arranged by size:
3467 2702 711
BsaBI GATnn'nnATC
Cuts at: 2453 6874 2453
Size: 4421 2459
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2931 4687 5069 5704
Size: 53 83 186 936 1398 1756 382 635
Cuts at: 5704 275
Size: 1451
Fragments arranged by size:
1756 1451 1398 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2076 2298 5313 5711 5985 6132 6543 2076
Size: 222 3015 398 274 147 411 2413
Fragments arranged by size:
3015 2413 411 398 274 222 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2696 2726 2768 2798 2921
Size: 30 165 30 1293 30 42 30 123
Cuts at: 2921 2951 1178
Size: 30 5107
Fragments arranged by size:
5107 1293 165 123 42 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2152 2576 2776 2909 2921 2926 3053 3055
Size: 966 424 200 133 12 5 127 2
Cuts at: 3055 3119 1186
Size: 64 4947
Fragments arranged by size:
4947 966 424 200 133 127 64 12
5 2
BseYI C'CCAG_C
Cuts at: 2192 2436 2680 2988 3723 4152 6083 6579 2192
Size: 244 244 308 735 429 1931 496 2493
Fragments arranged by size:
2493 1931 735 496 429 308 244 244
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2512 4097 1163
Size: 573 776 1585 3946
Fragments arranged by size:
3946 1585 776 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3124 4330 5091 6119 1540
Size: 275 186 1123 1206 761 1028 2301
Fragments arranged by size:
2301 1206 1123 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2030 2054 2646 2978 3609 4044 4080 4941 5026
Size: 24 592 332 631 435 36 861 85
Cuts at: 5026 6097 2030
Size: 1071 2813
Fragments arranged by size:
2813 1071 861 631 592 435 332 85
36 24
BsmI GAATG_Cn'
Cuts at: 2614 3759 4391 4484 2614
Size: 1145 632 93 5010
Fragments arranged by size:
5010 1145 632 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2030 2054 2270 2569 2646 2978 2985
Size: 399 240 24 216 299 77 332 7
Cuts at: 2985 3003 3017 3248 3323 3609 3709 3723 3751
Size: 18 14 231 75 286 100 14 28
Cuts at: 3751 3776 3818 3841 4044 4080 4199 4941 5026
Size: 25 42 23 203 36 119 742 85
Cuts at: 5026 6097 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 332 299 286 240
231 216 203 119 100 85 77 75
42 36 28 25 24 23 18 14
14 7
BspEI T'CCGG_A
Cuts at: 2076 2298 6543 2076
Size: 222 4245 2413
Fragments arranged by size:
4245 2413 222
BspHI T'CATG_A
Cuts at: 4769 6499 4769
Size: 1730 5150
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2902 4235 844
Size: 2058 1333 3489
Fragments arranged by size:
3489 2058 1333
BsrBI CCG'CTC
Cuts at: 1394 1823 3163 3570 4767 1394
Size: 429 1340 407 1197 3507
Fragments arranged by size:
3507 1340 1197 429 407
BsrDI GCAATG_nn'
Cuts at: 65 1248 5375 5549 65
Size: 1183 4127 174 1396
Fragments arranged by size:
4127 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2106 3377 3755 5524 5711 1700
Size: 110 296 1271 378 1769 187 2869
Fragments arranged by size:
2869 1769 1271 378 296 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6880
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6880
BssSI C'ACGA_G
Cuts at: 3562 4940 5952 3562
Size: 1378 1012 4490
Fragments arranged by size:
4490 1378 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2095 2656 4060 794
Size: 1301 561 1404 3614
Fragments arranged by size:
3614 1404 1301 561
BstBI TT'CG_AA
Cuts at: 5684 5684
Size: 6880
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6880
BstXI CCAn_nnnn'nTGG
Cuts at: 2752 3010 3841 2752
Size: 258 831 5791
Fragments arranged by size:
5791 831 258
BstYI r'GATC_y
Cuts at: 1972 2370 2409 2523 3022 3507 3733 4002 4056
Size: 398 39 114 499 485 226 269 54
Cuts at: 4056 4210 4577 4962 4979 6431 6517 6529 6875
Size: 154 367 385 17 1452 86 12 346
Cuts at: 6875 1972
Size: 1977
Fragments arranged by size:
1977 1452 499 485 398 385 367 346
269 226 154 114 86 54 39 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2136 2836 2884 3336 3527 4071
Size: 552 939 132 700 48 452 191 544
Cuts at: 4071 513
Size: 3322
Fragments arranged by size:
3322 939 700 552 544 452 191 132
48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2055 3859 487
Size: 683 56 829 1804 3508
Fragments arranged by size:
3508 1804 829 683 56
BtsI GCAGTG_nn'
Cuts at: 2612 3224 3441 4396 5190 5210 2612
Size: 612 217 955 794 20 4282
Fragments arranged by size:
4282 955 794 612 217 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6880
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2805 2840 529
Size: 35 2241 35 4569
Fragments arranged by size:
4569 2241 35 35
DraI TTT'AAA
Cuts at: 4184 4184
Size: 6880
DraIII CAC_nnn'GTG
Cuts at: 2656 3993 4033 2656
Size: 1337 40 5503
Fragments arranged by size:
5503 1337 40
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5887 809
Size: 507 4571 1802
Fragments arranged by size:
4571 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2044 2496 2839
Size: 54 1425 50 275 18 214 452 343
Cuts at: 2839 3121 3960 3984 4327 5216 6845 8
Size: 282 839 24 343 889 1629 43
Fragments arranged by size:
1629 1425 889 839 452 343 343 282
275 214 54 50 43 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 3121 4327 1537
Size: 275 1309 1206 4090
Fragments arranged by size:
4090 1309 1206 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3073 3185 3899 4810 1353
Size: 1720 112 714 911 3423
Fragments arranged by size:
3423 1720 911 714 112
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2585 3697 5467 5841 5987 387
Size: 1064 138 996 1112 1770 374 146 1280
Fragments arranged by size:
1770 1280 1112 1064 996 374 146 138
EcoICRI GAG'CTC
Cuts at: 2644 3607 4078 2644
Size: 963 471 5446
Fragments arranged by size:
5446 963 471
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2363 2483 2501 2923 3152 3185 3271
Size: 393 731 120 18 422 229 33 86
Cuts at: 3271 3617 3653 4124 4943 5540 6327 6595 1239
Size: 346 36 471 819 597 787 268 1524
Fragments arranged by size:
1524 819 787 731 597 471 422 393
346 268 229 120 86 36 33 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2378 3962 2378
Size: 1584 5296
EcoO109I rG'GnC_Cy
Cuts at: 2187 2565 2739 3014 3320 3720 3814 3815 2187
Size: 378 174 275 306 400 94 1 5252
Fragments arranged by size:
5252 400 378 306 275 174 94 1
EcoRI G'AATT_C
Cuts at: 4189 4567 4189
Size: 378 6502
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2566 2566
Size: 6880
FspI TGC'GCA
Cuts at: 5386 5386
Size: 6880
HaeII r_GCGC'y
Cuts at: 1678 3159 3280 3333 4013 6027 1678
Size: 1481 121 53 680 2014 2531
Fragments arranged by size:
2531 2014 1481 680 121 53
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2135 2147 2167 2179 2617
Size: 32 135 32 563 12 20 12 438
Cuts at: 2617 2649 2696 2728 2748 2768 2780 2800 2921
Size: 32 47 32 20 20 12 20 121
Cuts at: 2921 2953 3611 3643 5521 5553 5595 5627 1373
Size: 32 658 32 1878 32 42 32 2626
Fragments arranged by size:
2626 1878 658 563 438 135 121 47
42 32 32 32 32 32 32 32
32 20 20 20 20 12 12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 4224 4470 669
Size: 988 281 2286 246 3079
Fragments arranged by size:
3079 2286 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6880
HpaI GTT'AAC
Cuts at: 4470 4470
Size: 6880
KpnI G_GTAC'C
Cuts at: 4205 4205
Size: 6880
MfeI C'AATT_G
Cuts at: 4479 4479
Size: 6880
MluI A'CGCG_T
Cuts at: 6756 6756
Size: 6880
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5994 6178 1305
Size: 4689 184 2007
Fragments arranged by size:
4689 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2841 3962 10
Size: 54 1425 343 1009 1121 2928
Fragments arranged by size:
2928 1425 1121 1009 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2138 3233 4838 5197 5356 518
Size: 648 972 1095 1605 359 159 2042
Fragments arranged by size:
2042 1605 1095 972 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2436 2461 2577 2725 2798 4296 4973 6121
Size: 830 25 116 148 73 1498 677 1148
Cuts at: 6121 6366 1606
Size: 245 2120
Fragments arranged by size:
2120 1498 1148 830 677 245 148 116
73 25
NaeI GCC'GGC
Cuts at: 2108 3379 3757 2108
Size: 1271 378 5231
Fragments arranged by size:
5231 1271 378
NcoI C'CATG_G
Cuts at: 513 1065 2004 2136 2884 513
Size: 552 939 132 748 4509
Fragments arranged by size:
4509 939 748 552 132
NdeI CA'TA_TG
Cuts at: 387 2011 387
Size: 1624 5256
NgoMIV G'CCGG_C
Cuts at: 2106 3377 3755 2106
Size: 1271 378 5231
Fragments arranged by size:
5231 1271 378
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6880
NotI GC'GGCC_GC
Cuts at: 4327 4327
Size: 6880
NspI r_CATG'y
Cuts at: 1132 1229 2478 4238 5783 1132
Size: 97 1249 1760 1545 2229
Fragments arranged by size:
2229 1760 1545 1249 97
PasI CC'CwG_GG
Cuts at: 1348 3010 1348
Size: 1662 5218
PciI A'CATG_T
Cuts at: 2474 5779 2474
Size: 3305 3575
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3595 3993 4033 1108
Size: 2487 398 40 3955
Fragments arranged by size:
3955 2487 398 40
PfoI T'CCnGG_A
Cuts at: 4080 4080
Size: 6880
PmeI GTTT'AAAC
Cuts at: 4184 4184
Size: 6880
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4079 4111 4949 4981 6462 6494 4079
Size: 32 838 32 1481 32 4465
Fragments arranged by size:
4465 1481 838 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2187 2739 2187
Size: 552 6328
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6880
PsiI TTA'TAA
Cuts at: 2277 4450 2277
Size: 2173 4707
PspOMI G'GGCC_C
Cuts at: 2565 2999 3013 3319 3719 3814 2565
Size: 434 14 306 400 95 5631
Fragments arranged by size:
5631 434 400 306 95 14
PstI C_TGCA'G
Cuts at: 830 2091 2364 4017 4232 830
Size: 1261 273 1653 215 3478
Fragments arranged by size:
3478 1653 1261 273 215
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6880
PvuII CAG'CTG
Cuts at: 2461 2725 2798 2461
Size: 264 73 6543
Fragments arranged by size:
6543 264 73
SacI G_AGCT'C
Cuts at: 2646 3609 4080 2646
Size: 963 471 5446
Fragments arranged by size:
5446 963 471
SalI G'TCGA_C
Cuts at: 1936 4222 1936
Size: 2286 4594
SanDI GG'GwC_CC
Cuts at: 2187 2739 2187
Size: 552 6328
SapI GCTCTTCn'nnn_
Cuts at: 3185 3899 3185
Size: 714 6166
SbfI CC_TGCA'GG
Cuts at: 4232 4232
Size: 6880
ScaI AGT'ACT
Cuts at: 5128 6840 5128
Size: 1712 5168
SexAI A'CCwGG_T
Cuts at: 2652 2652
Size: 6880
SfcI C'TryA_G
Cuts at: 826 1045 2087 2360 2419 3212 4013 4228 5363
Size: 219 1042 273 59 793 801 215 1135
Cuts at: 5363 6044 6235 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1042 801 793 681 273 219
215 191 59
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6880
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6880
SmaI CCC'GGG
Cuts at: 1872 3656 3752 3820 4207 1872
Size: 1784 96 68 387 4545
Fragments arranged by size:
4545 1784 387 96 68
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4984 5885 6147 820
Size: 197 926 3041 901 262 1553
Fragments arranged by size:
3041 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4671 493
Size: 4178 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6880
SphI G_CATG'C
Cuts at: 1132 4238 1132
Size: 3106 3774
SrfI GCCC'GGGC
Cuts at: 3752 3752
Size: 6880
SspI AAT'ATT
Cuts at: 5 52 4804 5
Size: 47 4752 2081
Fragments arranged by size:
4752 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6880
StyI C'CwwG_G
Cuts at: 513 1065 2004 2136 2206 2745 2884 3018 3612
Size: 552 939 132 70 539 139 134 594
Cuts at: 3612 3963 4117 4319 513
Size: 351 154 202 3074
Fragments arranged by size:
3074 939 594 552 539 351 202 154
139 134 132 70
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2213 3002 3744 4166 4907 4924 5077
Size: 1255 633 789 742 422 741 17 153
Cuts at: 5077 5262 5707 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 789 742 741 633 445 422
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5126 6838 96
Size: 274 80 33 51 1443 3149 1712 138
Fragments arranged by size:
3149 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3251 3283 6840 6872 3251
Size: 32 3557 32 3259
Fragments arranged by size:
3557 3259 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2708 1316
Size: 1392 5488
XbaI T'CTAG_A
Cuts at: 4216 4216
Size: 6880
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6880
XmaI C'CCGG_G
Cuts at: 1870 3654 3750 3818 4205 1870
Size: 1784 96 68 387 4545
Fragments arranged by size:
4545 1784 387 96 68
XmnI GAAnn'nnTTC
Cuts at: 2306 2546 4022 5009 2306
Size: 240 1476 987 4177
Fragments arranged by size:
4177 1476 987 240
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4688 276
Size: 53 83 186 936 3154 2468
Fragments arranged by size:
3154 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BfrBI BsiWI
BsmBI BstZ17I Bsu36I EcoRV FseI FspAI KasI NarI
NruI NsiI PacI PmlI PspXI PsrI RsrII SacII
SfiI SfoI SwaI XcmI