Restriction map for
pFN21AA1522
(Circular) (Six-base) MAPSORT of: pFN21AA1522.seq Check: 7531 from: 1 to: 7843
pFN21AA1522 7843 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5653 278
Size: 53 83 186 936 4117 2468
Fragments arranged by size:
4117 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2757 5186 1937
Size: 820 2429 4594
Fragments arranged by size:
4594 2429 820
Acc65I G'GTAC_C
Cuts at: 2792 3214 3535 5164 2792
Size: 422 321 1629 5471
Fragments arranged by size:
5471 1629 422 321
AclI AA'CG_TT
Cuts at: 1563 5970 6343 1563
Size: 4407 373 3063
Fragments arranged by size:
4407 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3362 4260 5906 7290 7558 1632
Size: 1730 898 1646 1384 268 1917
Fragments arranged by size:
1917 1730 1646 1384 898 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7646
AflIII A'CryG_T
Cuts at: 1203 6742 7719 1203
Size: 5539 977 1327
Fragments arranged by size:
5539 1327 977
AgeI A'CCGG_T
Cuts at: 4438 6674 4438
Size: 2236 5607
AhdI GACnn_n'nnGTC
Cuts at: 6572 6572
Size: 7843
AleI CACnn'nnGTG
Cuts at: 1166 3134 1166
Size: 1968 5875
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7843
AlwNI CAG_nnn'CTG
Cuts at: 2061 2099 2156 2278 2692 2837 2861 3404 4136
Size: 38 57 122 414 145 24 543 732
Cuts at: 4136 4204 4328 4391 4631 5586 7158 2061
Size: 68 124 63 240 955 1572 2746
Fragments arranged by size:
2746 1572 955 732 543 414 240 145
124 122 68 63 57 38 24
ApaI G_GGCC'C
Cuts at: 2674 2803 4606 5093 2674
Size: 129 1803 487 5424
Fragments arranged by size:
5424 1803 487 129
ApaLI G'TGCA_C
Cuts at: 2607 5900 7056 2607
Size: 3293 1156 3394
Fragments arranged by size:
3394 3293 1156
ApoI r'AATT_y
Cuts at: 1493 5152 5378 5530 7639 1493
Size: 3659 226 152 2109 1697
Fragments arranged by size:
3659 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6397 160
Size: 872 5365 1606
Fragments arranged by size:
5365 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7843
AvaI C'yCGr_G
Cuts at: 1870 1943 2046 2595 2649 2673 2796 3613 4348
Size: 73 103 549 54 24 123 817 735
Cuts at: 4348 4986 5113 5168 1870
Size: 638 127 55 4545
Fragments arranged by size:
4545 817 735 638 549 127 123 103
73 55 54 24
AvrII C'CTAG_G
Cuts at: 2965 2965
Size: 7843
BamHI G'GATC_C
Cuts at: 5173 5540 5173
Size: 367 7476
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2581 2654 2792 3214 3535
Size: 325 217 627 794 73 138 422 321
Cuts at: 3535 3564 3856 4117 5164 6619 618
Size: 29 292 261 1047 1455 1842
Fragments arranged by size:
1842 1455 1047 794 627 422 325 321
292 261 217 138 73 29
BanII G_rGCy'C
Cuts at: 1391 2247 2396 2602 2674 2803 2871 2879 3194
Size: 856 149 206 72 129 68 8 315
Cuts at: 3194 3248 3899 4149 4555 4606 4650 4793 4998
Size: 54 651 250 406 51 44 143 205
Cuts at: 4998 5025 5093 5120 5162 1391
Size: 27 68 27 42 4072
Fragments arranged by size:
4072 856 651 406 315 250 206 205
149 143 129 72 68 68 54 51
44 42 27 27 8
BbeI G_GCGC'C
Cuts at: 3568 3568
Size: 7843
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2176 2352 928
Size: 971 277 176 6419
Fragments arranged by size:
6419 971 277 176
BbvCI CC'TCA_GC
Cuts at: 3270 4140 4321 3270
Size: 870 181 6792
Fragments arranged by size:
6792 870 181
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6034 6068 1903
Size: 34 4097 34 3678
Fragments arranged by size:
4097 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2327 3602 5735 6945 2327
Size: 1275 2133 1210 3225
Fragments arranged by size:
3225 2133 1275 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7843
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2652 3409 4278
Size: 107 122 71 631 605 980 757 869
Cuts at: 4278 4766 5115 6454 136
Size: 488 349 1339 1525
Fragments arranged by size:
1525 1339 980 869 757 631 605 488
349 122 107 71
BglII A'GATC_T
Cuts at: 7838 7838
Size: 7843
BlpI GC'TnA_GC
Cuts at: 3394 4710 4992 5260 3394
Size: 1316 282 268 5977
Fragments arranged by size:
5977 1316 282 268
Bme1580I G_kGCm'C
Cuts at: 1790 2611 2674 2803 4606 5093 5904 7060 1790
Size: 821 63 129 1803 487 811 1156 2573
Fragments arranged by size:
2573 1803 1156 821 811 487 129 63
BmgBI CAC'GTC
Cuts at: 1318 2956 3711 3753 1318
Size: 1638 755 42 5408
Fragments arranged by size:
5408 1638 755 42
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2187 3246 4577
Size: 416 53 83 399 24 770 1059 1331
Cuts at: 4577 4619 4744 4971 5574 6532 442
Size: 42 125 227 603 958 1753
Fragments arranged by size:
1753 1331 1059 958 770 603 416 399
227 125 83 53 42 24
BmtI G_CTAG'C
Cuts at: 1056 3204 1056
Size: 2148 5695
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3132 3264 3132
Size: 132 7711
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2714 3043 3079 3127 3586 4353 4527 4829
Size: 1475 329 36 48 459 767 174 302
Cuts at: 4829 6503 1239
Size: 1674 2579
Fragments arranged by size:
2579 1674 1475 767 459 329 302 174
48 36
Bpu10I CC'TnA_GC
Cuts at: 2873 3270 3429 3435 4140 4260 4321 2873
Size: 397 159 6 705 120 61 6395
Fragments arranged by size:
6395 705 397 159 120 61 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4652 4687 5968 6833 7131 4652
Size: 35 1281 865 298 5364
Fragments arranged by size:
5364 1281 865 298 35
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4823 6506 882
Size: 617 3324 1683 2219
Fragments arranged by size:
3324 2219 1683 617
BsaAI yAC'GTr
Cuts at: 493 1204 2383 5634 493
Size: 711 1179 3251 2702
Fragments arranged by size:
3251 2702 1179 711
BsaBI GATnn'nnATC
Cuts at: 7837 7837
Size: 7843
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2500 3565 3963 5650
Size: 53 83 186 936 967 1065 398 1687
Cuts at: 5650 6032 6667 275
Size: 382 635 1451
Fragments arranged by size:
1687 1451 1065 967 936 635 398 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 4438 4869 6276 6674 6948 7095 7506 4438
Size: 431 1407 398 274 147 411 4775
Fragments arranged by size:
4775 1407 431 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2339 2369 2399 2429 3074
Size: 30 165 30 936 30 30 30 645
Cuts at: 3074 3104 3625 3655 3988 4018 4993 5023 1178
Size: 30 521 30 333 30 975 30 3998
Fragments arranged by size:
3998 975 936 645 521 333 165 30
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3040 3082 3148 3730 3757 3760 3834 4157
Size: 1854 42 66 582 27 3 74 323
Cuts at: 4157 4208 4247 4309 4356 4630 4998 5111 1186
Size: 51 39 62 47 274 368 113 3918
Fragments arranged by size:
3918 1854 582 368 323 274 113 74
66 62 51 47 42 39 27 3
BseYI C'CCAG_C
Cuts at: 2036 2292 2527 3019 3037 3453 3520 3531 3684
Size: 256 235 492 18 416 67 11 153
Cuts at: 3684 3778 3904 4384 4561 7046 7542 2036
Size: 94 126 480 177 2485 496 2337
Fragments arranged by size:
2485 2337 496 492 480 416 256 235
177 153 126 94 67 18 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2207 2567 2990 1163
Size: 573 471 360 423 6016
Fragments arranged by size:
6016 573 471 423 360
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2078 2090 2644 5293 6054 7082
Size: 275 186 77 12 554 2649 761 1028
Cuts at: 7082 1540
Size: 2301
Fragments arranged by size:
2649 2301 1028 761 554 275 186 77
12
BsiHKAI G_wGCw'C
Cuts at: 2172 2396 2522 2611 3194 4543 4711 5904 5989
Size: 224 126 89 583 1349 168 1193 85
Cuts at: 5989 7060 2172
Size: 1071 2955
Fragments arranged by size:
2955 1349 1193 1071 583 224 168 126
89 85
BsiWI C'GTAC_G
Cuts at: 2620 2620
Size: 7843
BsmI GAATG_Cn'
Cuts at: 3291 5354 5447 3291
Size: 2063 93 5687
Fragments arranged by size:
5687 2063 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3061 3717 3742 3061
Size: 656 25 7162
Fragments arranged by size:
7162 656 25
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2172 2247 2396 2522 2602 2611 2674
Size: 399 382 75 149 126 80 9 63
Cuts at: 2674 2803 2871 2879 3194 3248 3899 4149 4543
Size: 129 68 8 315 54 651 250 394
Cuts at: 4543 4555 4606 4650 4711 4793 4998 5025 5093
Size: 12 51 44 61 82 205 27 68
Cuts at: 5093 5120 5162 5904 5989 7060 1391
Size: 27 42 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 651 399 394 382 315
250 205 149 129 126 85 82 80
75 68 68 63 61 54 51 44
42 27 27 12 9 8
BspEI T'CCGG_A
Cuts at: 4869 7506 4869
Size: 2637 5206
BspHI T'CATG_A
Cuts at: 5732 7462 5732
Size: 1730 6113
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3647 5198 844
Size: 2803 1551 3489
Fragments arranged by size:
3489 2803 1551
BsrBI CCG'CTC
Cuts at: 1394 1823 2250 2424 2512 3033 3795 4813 5730
Size: 429 427 174 88 521 762 1018 917
Cuts at: 5730 1394
Size: 3507
Fragments arranged by size:
3507 1018 917 762 521 429 427 174
88
BsrDI GCAATG_nn'
Cuts at: 65 1248 6338 6512 65
Size: 1183 5090 174 1396
Fragments arranged by size:
5090 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2089 2781 4438 6487 6674 1700
Size: 110 279 692 1657 2049 187 2869
Fragments arranged by size:
2869 2049 1657 692 279 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7843
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7843
BssSI C'ACGA_G
Cuts at: 5903 6915 5903
Size: 1012 6831
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2099 2545 2615 2861 4124 4519 794
Size: 1305 446 70 246 1263 395 4118
Fragments arranged by size:
4118 1305 1263 446 395 246 70
BstBI TT'CG_AA
Cuts at: 6647 6647
Size: 7843
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7843
BstXI CCAn_nnnn'nTGG
Cuts at: 3652 3652
Size: 7843
BstYI r'GATC_y
Cuts at: 1972 4156 5173 5540 5925 5942 7394 7480 7492
Size: 2184 1017 367 385 17 1452 86 12
Cuts at: 7492 7838 1972
Size: 346 1977
Fragments arranged by size:
2184 1977 1452 1017 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2564 2659 4191 4642 4728 4917 2564
Size: 95 1532 451 86 189 5490
Fragments arranged by size:
5490 1532 451 189 95 86
BtgI C'CryG_G
Cuts at: 513 1065 2004 2299 2787 3572 4209 4533 4739
Size: 552 939 295 488 785 637 324 206
Cuts at: 4739 513
Size: 3617
Fragments arranged by size:
3617 939 785 637 552 488 324 295
206
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7104
Fragments arranged by size:
7104 683 56
BtsI GCAGTG_nn'
Cuts at: 2166 2838 3241 3264 3307 3405 4007 4592 5359
Size: 672 403 23 43 98 602 585 767
Cuts at: 5359 6153 6173 2166
Size: 794 20 3836
Fragments arranged by size:
3836 794 767 672 602 585 403 98
43 23 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7843
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3792 3827 4441 4476 529
Size: 35 3228 35 614 35 3896
Fragments arranged by size:
3896 3228 614 35 35 35
DraI TTT'AAA
Cuts at: 5147 5147
Size: 7843
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3833 6850 809
Size: 507 2517 3017 1802
Fragments arranged by size:
3017 2517 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2087 2140 2641
Size: 54 1425 50 275 18 257 53 501
Cuts at: 2641 2779 2880 3012 3316 3464 4251 4416 4683
Size: 138 101 132 304 148 787 165 267
Cuts at: 4683 4767 4889 5139 5290 6179 7808 8
Size: 84 122 250 151 889 1629 43
Fragments arranged by size:
1629 1425 889 787 501 304 275 267
257 250 165 151 148 138 132 122
101 84 54 53 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2087 2641 5290 1537
Size: 275 275 554 2649 4090
Fragments arranged by size:
4090 2649 554 275 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2211 3720 3748 5773 1353
Size: 858 1509 28 2025 3423
Fragments arranged by size:
3423 2025 1509 858 28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2530 2714 2720 4365 4390 5075
Size: 1064 138 941 184 6 1645 25 685
Cuts at: 5075 6430 6804 6950 387
Size: 1355 374 146 1280
Fragments arranged by size:
1645 1355 1280 1064 941 685 374 184
146 138 25 6
EcoICRI GAG'CTC
Cuts at: 2394 3192 2394
Size: 798 7045
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2714 3043 3079 3127 3362 3586 4260
Size: 393 1082 329 36 48 235 224 674
Cuts at: 4260 4353 4527 4829 5906 6503 7290 7558 1239
Size: 93 174 302 1077 597 787 268 1524
Fragments arranged by size:
1524 1082 1077 787 674 597 393 329
302 268 235 224 174 93 48 36
EcoO109I rG'GnC_Cy
Cuts at: 2032 2135 2670 2800 2858 3649 3659 3729 4201
Size: 103 535 130 58 791 10 70 472
Cuts at: 4201 4271 4602 4603 4900 2032
Size: 70 331 1 297 4975
Fragments arranged by size:
4975 791 535 472 331 297 130 103
70 70 58 10 1
EcoRI G'AATT_C
Cuts at: 5152 5530 5152
Size: 378 7465
FspI TGC'GCA
Cuts at: 2984 6349 2984
Size: 3365 4478
HaeII r_GCGC'y
Cuts at: 1678 2693 2718 2743 3046 3568 6990 1678
Size: 1015 25 25 303 522 3422 2531
Fragments arranged by size:
3422 2531 1015 522 303 25 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2339 2371 2399 2431 4106
Size: 32 135 32 767 32 28 32 1675
Cuts at: 4106 4138 6484 6516 6558 6590 1373
Size: 32 2346 32 42 32 2626
Fragments arranged by size:
2626 2346 1675 767 135 42 32 32
32 32 32 32 32 28
HincII GTy'rAC
Cuts at: 669 1657 1938 5187 5433 669
Size: 988 281 3249 246 3079
Fragments arranged by size:
3249 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7843
HpaI GTT'AAC
Cuts at: 5433 5433
Size: 7843
KasI G'GCGC_C
Cuts at: 3564 3564
Size: 7843
KpnI G_GTAC'C
Cuts at: 2796 3218 3539 5168 2796
Size: 422 321 1629 5471
Fragments arranged by size:
5471 1629 422 321
MfeI C'AATT_G
Cuts at: 5442 5442
Size: 7843
MluI A'CGCG_T
Cuts at: 7719 7719
Size: 7843
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2482 3150 4687 6957 7141 1305
Size: 1177 668 1537 2270 184 2007
Fragments arranged by size:
2270 2007 1537 1177 668 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2142 4253 4418 10
Size: 54 1425 343 310 2111 165 3435
Fragments arranged by size:
3435 2111 1425 343 310 165 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2127 3134 5801 6160 6319 518
Size: 648 961 1007 2667 359 159 2042
Fragments arranged by size:
2667 2042 1007 961 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2040 2973 2997 3388 3457 3531 3908 4807
Size: 434 933 24 391 69 74 377 899
Cuts at: 4807 4849 4888 5083 5259 5936 7084 7329 1606
Size: 42 39 195 176 677 1148 245 2120
Fragments arranged by size:
2120 1148 933 899 677 434 391 377
245 195 176 74 69 42 39 24
NaeI GCC'GGC
Cuts at: 2091 2091
Size: 7843
NarI GG'CG_CC
Cuts at: 3565 3565
Size: 7843
NcoI C'CATG_G
Cuts at: 513 1065 2004 2299 4209 513
Size: 552 939 295 1910 4147
Fragments arranged by size:
4147 1910 939 552 295
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7843
NgoMIV G'CCGG_C
Cuts at: 2089 2089
Size: 7843
NheI G'CTAG_C
Cuts at: 1052 3200 1052
Size: 2148 5695
NotI GC'GGCC_GC
Cuts at: 5290 5290
Size: 7843
NspI r_CATG'y
Cuts at: 1132 1229 2546 2933 4415 5201 6746 1132
Size: 97 1317 387 1482 786 1545 2229
Fragments arranged by size:
2229 1545 1482 1317 786 387 97
PasI CC'CwG_GG
Cuts at: 1348 3226 3725 3820 4406 4470 1348
Size: 1878 499 95 586 64 4721
Fragments arranged by size:
4721 1878 586 499 95 64
PciI A'CATG_T
Cuts at: 6742 6742
Size: 7843
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3531 1108
Size: 2423 5420
PfoI T'CCnGG_A
Cuts at: 2327 4543 2327
Size: 2216 5627
PmeI GTTT'AAAC
Cuts at: 5147 5147
Size: 7843
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5912 5944 7425 7457 5912
Size: 32 1481 32 6298
Fragments arranged by size:
6298 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3659 4201 3659
Size: 542 7301
PshAI GACnn'nnGTC
Cuts at: 1613 3966 1613
Size: 2353 5490
PsiI TTA'TAA
Cuts at: 5413 5413
Size: 7843
PspOMI G'GGCC_C
Cuts at: 2670 2799 4602 5089 2670
Size: 129 1803 487 5424
Fragments arranged by size:
5424 1803 487 129
PspXI vC'TCGA_Gb
Cuts at: 3613 4986 3613
Size: 1373 6470
PstI C_TGCA'G
Cuts at: 830 2161 2366 2688 2739 2866 2978 3002 4014
Size: 1331 205 322 51 127 112 24 1012
Cuts at: 4014 4270 4515 4679 4806 5082 5195 830
Size: 256 245 164 127 276 113 3478
Fragments arranged by size:
3478 1331 1012 322 276 256 245 205
164 127 127 113 112 51 24
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7843
PvuII CAG'CTG
Cuts at: 3457 3531 3908 4807 5083 3457
Size: 74 377 899 276 6217
Fragments arranged by size:
6217 899 377 276 74
RsrII CG'GwC_CG
Cuts at: 4660 4721 4660
Size: 61 7782
SacI G_AGCT'C
Cuts at: 2396 3194 2396
Size: 798 7045
SalI G'TCGA_C
Cuts at: 1936 5185 1936
Size: 3249 4594
SapI GCTCTTCn'nnn_
Cuts at: 2211 2211
Size: 7843
SbfI CC_TGCA'GG
Cuts at: 2688 4270 5195 2688
Size: 1582 925 5336
Fragments arranged by size:
5336 1582 925
ScaI AGT'ACT
Cuts at: 6091 7803 6091
Size: 1712 6131
SfcI C'TryA_G
Cuts at: 826 1045 2157 2362 2684 2735 2862 2946 2974
Size: 219 1112 205 322 51 127 84 28
Cuts at: 2974 2998 4010 4266 4447 4511 4675 4802 5078
Size: 24 1012 256 181 64 164 127 276
Cuts at: 5078 5191 6326 7007 7198 826
Size: 113 1135 681 191 1471
Fragments arranged by size:
1471 1135 1112 1012 681 322 276 256
219 205 191 181 164 127 127 113
84 64 51 28 24
SfoI GGC'GCC
Cuts at: 3566 3566
Size: 7843
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7843
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7843
SmaI CCC'GGG
Cuts at: 1872 2048 2597 2675 2798 4350 5115 5170 1872
Size: 176 549 78 123 1552 765 55 4545
Fragments arranged by size:
4545 1552 765 549 176 123 78 55
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3613 4667 4702 4986 5947 6848
Size: 197 926 1670 1054 35 284 961 901
Cuts at: 6848 7110 820
Size: 262 1553
Fragments arranged by size:
1670 1553 1054 961 926 901 284 262
197 35
SnaBI TAC'GTA
Cuts at: 493 5634 493
Size: 5141 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7843
SphI G_CATG'C
Cuts at: 1132 5201 1132
Size: 4069 3774
SspI AAT'ATT
Cuts at: 5 52 5767 5
Size: 47 5715 2081
Fragments arranged by size:
5715 2081 47
StuI AGG'CCT
Cuts at: 1517 3275 4673 4698 1517
Size: 1758 1398 25 4662
Fragments arranged by size:
4662 1758 1398 25
StyI C'CwwG_G
Cuts at: 513 1065 2004 2103 2299 2965 3645 3852 4209
Size: 552 939 99 196 666 680 207 357
Cuts at: 4209 4785 5282 513
Size: 576 497 3074
Fragments arranged by size:
3074 939 680 666 576 552 497 357
207 196 99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2324 3099 3484 3529 3812 4102 4526
Size: 1255 744 775 385 45 283 290 424
Cuts at: 4526 5048 5870 5887 6040 6225 6670 325
Size: 522 822 17 153 185 445 1498
Fragments arranged by size:
1498 1255 822 775 744 522 445 424
385 290 283 185 153 45 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6089 7801 96
Size: 274 80 33 51 1443 4112 1712 138
Fragments arranged by size:
4112 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2560 2592 3074 3104 3106 3136 3625 3657 7803
Size: 32 482 30 2 30 489 32 4146
Cuts at: 7803 7835 2560
Size: 32 2568
Fragments arranged by size:
4146 2568 489 482 32 32 32 30
30 2
Tth111I GACn'n_nGTC
Cuts at: 1316 2180 2753 1316
Size: 864 573 6406
Fragments arranged by size:
6406 864 573
XbaI T'CTAG_A
Cuts at: 5179 5179
Size: 7843
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2380 3233 3528 4564 2380
Size: 853 295 1036 5659
Fragments arranged by size:
5659 1036 853 295
XhoI C'TCGA_G
Cuts at: 1943 3613 4986 1943
Size: 1670 1373 4800
Fragments arranged by size:
4800 1670 1373
XmaI C'CCGG_G
Cuts at: 1870 2046 2595 2673 2796 4348 5113 5168 1870
Size: 176 549 78 123 1552 765 55 4545
Fragments arranged by size:
4545 1552 765 549 176 123 78 55
XmnI GAAnn'nnTTC
Cuts at: 5972 5972
Size: 7843
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5651 276
Size: 53 83 186 936 4117 2468
Fragments arranged by size:
4117 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BfrBI BstZ17I DraIII
EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI
PmlI PsrI SacII SanDI SexAI SfiI SrfI SwaI