Restriction map for
pFN21AA1521
(Circular) (Six-base) MAPSORT of: pFN21AA1521.seq Check: 6441 from: 1 to: 9139
pFN21AA1521 9139 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4025 4025
Size: 9139
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3981 6949 278
Size: 53 83 186 936 2445 2968 2468
Fragments arranged by size:
2968 2468 2445 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5665 6323 6482 1937
Size: 3728 658 159 4594
Fragments arranged by size:
4594 3728 658 159
Acc65I G'GTAC_C
Cuts at: 6460 6460
Size: 9139
AclI AA'CG_TT
Cuts at: 1563 4945 7266 7639 1563
Size: 3382 2321 373 3063
Fragments arranged by size:
3382 3063 2321 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2214 2438 2521 2583 3093 3753 4905 5526
Size: 582 224 83 62 510 660 1152 621
Cuts at: 5526 6074 7202 8586 8854 1632
Size: 548 1128 1384 268 1917
Fragments arranged by size:
1917 1384 1152 1128 660 621 582 548
510 268 224 83 62
AflII C'TTAA_G
Cuts at: 820 1017 2108 820
Size: 197 1091 7851
Fragments arranged by size:
7851 1091 197
AflIII A'CryG_T
Cuts at: 1203 4053 8038 9015 1203
Size: 2850 3985 977 1327
Fragments arranged by size:
3985 2850 1327 977
AgeI A'CCGG_T
Cuts at: 7970 7970
Size: 9139
AhdI GACnn_n'nnGTC
Cuts at: 7868 7868
Size: 9139
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9139
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3488 4047 1184
Size: 2304 559 6276
Fragments arranged by size:
6276 2304 559
AlwNI CAG_nnn'CTG
Cuts at: 4040 5312 6882 8454 4040
Size: 1272 1570 1572 4725
Fragments arranged by size:
4725 1572 1570 1272
ApaLI G'TGCA_C
Cuts at: 5073 5250 7196 8352 5073
Size: 177 1946 1156 5860
Fragments arranged by size:
5860 1946 1156 177
ApoI r'AATT_y
Cuts at: 1493 2229 2297 2477 2795 3259 3906 5804 6410
Size: 736 68 180 318 464 647 1898 606
Cuts at: 6410 6448 6674 6826 8935 1493
Size: 38 226 152 2109 1697
Fragments arranged by size:
2109 1898 1697 736 647 606 464 318
226 180 152 68 38
AseI AT'TA_AT
Cuts at: 160 1032 3003 5514 7693 160
Size: 872 1971 2511 2179 1606
Fragments arranged by size:
2511 2179 1971 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9139
AvaI C'yCGr_G
Cuts at: 1870 1943 4855 6174 6464 1870
Size: 73 2912 1319 290 4545
Fragments arranged by size:
4545 2912 1319 290 73
AvrII C'CTAG_G
Cuts at: 2504 2504
Size: 9139
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4245 4278 4245
Size: 33 9106
BamHI G'GATC_C
Cuts at: 6469 6836 6469
Size: 367 8772
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4433 6460 7915 618
Size: 325 217 627 2646 2027 1455 1842
Fragments arranged by size:
2646 2027 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2723 4712 5638 6088 6458 1391
Size: 1332 1989 926 450 370 4072
Fragments arranged by size:
4072 1989 1332 926 450 370
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3177 4359 4491 5472 928
Size: 971 1278 1182 132 981 4595
Fragments arranged by size:
4595 1278 1182 981 971 132
BbvCI CC'TCA_GC
Cuts at: 3393 5091 5616 3393
Size: 1698 525 6916
Fragments arranged by size:
6916 1698 525
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5831 5865 7330 7364 1903
Size: 34 3894 34 1465 34 3678
Fragments arranged by size:
3894 3678 1465 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7031 8241 7031
Size: 1210 7929
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9139
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7750 136
Size: 107 122 71 631 605 6078 1525
Fragments arranged by size:
6078 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4361 9134 4361
Size: 4773 4366
BlpI GC'TnA_GC
Cuts at: 6556 6556
Size: 9139
Bme1580I G_kGCm'C
Cuts at: 1790 4438 5077 5254 7200 8356 1790
Size: 2648 639 177 1946 1156 2573
Fragments arranged by size:
2648 2573 1946 1156 639 177
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9139
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4244 5133 5408
Size: 416 53 83 399 24 2827 889 275
Cuts at: 5408 6870 7828 442
Size: 1462 958 1753
Fragments arranged by size:
2827 1753 1462 958 889 416 399 275
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 6357 1056
Size: 5301 3838
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2698 2700 2737 4390 5400 2698
Size: 2 37 1653 1010 6437
Fragments arranged by size:
6437 1653 1010 37 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2373 3303 3387 3399 3567 3988 4238 4629
Size: 1134 930 84 12 168 421 250 391
Cuts at: 4629 4779 6276 6387 7799 1239
Size: 150 1497 111 1412 2579
Fragments arranged by size:
2579 1497 1412 1134 930 421 391 250
168 150 111 84 12
Bpu10I CC'TnA_GC
Cuts at: 3393 3893 5082 5091 5616 5853 6227 3393
Size: 500 1189 9 525 237 374 6305
Fragments arranged by size:
6305 1189 525 500 374 237 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2027 2323 3495 4855 5798 7264 8129 8427 2027
Size: 296 1172 1360 943 1466 865 298 2739
Fragments arranged by size:
2739 1466 1360 1172 943 865 298 296
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3164 3956 4729 7802 882
Size: 617 1665 792 773 3073 2219
Fragments arranged by size:
3073 2219 1665 792 773 617
BsaAI yAC'GTr
Cuts at: 493 1204 6930 493
Size: 711 5726 2702
Fragments arranged by size:
5726 2702 711
BsaBI GATnn'nnATC
Cuts at: 2640 4014 5529 9133 2640
Size: 1374 1515 3604 2646
Fragments arranged by size:
3604 2646 1515 1374
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3978 4765 6946 7328
Size: 53 83 186 936 2445 787 2181 382
Cuts at: 7328 7963 275
Size: 635 1451
Fragments arranged by size:
2445 2181 1451 936 787 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4731 5973 7572 7970 8244 8391 8802 4731
Size: 1242 1599 398 274 147 411 5068
Fragments arranged by size:
5068 1599 1242 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3774 3804 4438 4468 4930
Size: 30 165 30 2371 30 634 30 462
Cuts at: 4930 4960 1178
Size: 30 5357
Fragments arranged by size:
5357 2371 634 462 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2329 2533 4214 4443 4858 4890 6386 1186
Size: 1143 204 1681 229 415 32 1496 3939
Fragments arranged by size:
3939 1681 1496 1143 415 229 204 32
BseYI C'CCAG_C
Cuts at: 3573 4283 4751 5057 8342 8838 3573
Size: 710 468 306 3285 496 3874
Fragments arranged by size:
3874 3285 710 496 468 306
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3183 4093 4390 4815 6117 6206 6350
Size: 573 1447 910 297 425 1302 89 144
Cuts at: 6350 1163
Size: 3952
Fragments arranged by size:
3952 1447 1302 910 573 425 297 144
89
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6589 7350 8378 1540
Size: 275 186 4588 761 1028 2301
Fragments arranged by size:
4588 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4712 5077 5254 6088 6209 7200 7285 8356 4712
Size: 365 177 834 121 991 85 1071 5495
Fragments arranged by size:
5495 1071 991 834 365 177 121 85
BsmI GAATG_Cn'
Cuts at: 4661 4675 5122 6650 6743 4661
Size: 14 447 1528 93 7057
Fragments arranged by size:
7057 1528 447 93 14
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2723 4438 4712 5077 5254 5638 6088
Size: 399 933 1715 274 365 177 384 450
Cuts at: 6088 6209 6458 7200 7285 8356 1391
Size: 121 249 742 85 1071 2174
Fragments arranged by size:
2174 1715 1071 933 742 450 399 384
365 274 249 177 121 85
BspEI T'CCGG_A
Cuts at: 4731 8802 4731
Size: 4071 5068
BspHI T'CATG_A
Cuts at: 5182 6037 7028 8758 5182
Size: 855 991 1730 5563
Fragments arranged by size:
5563 1730 991 855
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3022 4025 6494 844
Size: 2178 1003 2469 3489
Fragments arranged by size:
3489 2469 2178 1003
BsrBI CCG'CTC
Cuts at: 1394 1823 6260 7026 1394
Size: 429 4437 766 3507
Fragments arranged by size:
4437 3507 766 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2922 3066 5157 5895 7634 7808 65
Size: 1183 1674 144 2091 738 1739 174 1396
Fragments arranged by size:
2091 1739 1674 1396 1183 738 174 144
BsrFI r'CCGG_y
Cuts at: 1700 1810 4761 7783 7970 1700
Size: 110 2951 3022 187 2869
Fragments arranged by size:
3022 2951 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2865 96
Size: 2769 6370
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9139
BssSI C'ACGA_G
Cuts at: 7199 8211 7199
Size: 1012 8127
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2144 4040 794
Size: 1350 1896 5893
Fragments arranged by size:
5893 1896 1350
BstBI TT'CG_AA
Cuts at: 4699 7943 4699
Size: 3244 5895
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9139
BstXI CCAn_nnnn'nTGG
Cuts at: 2233 2233
Size: 9139
BstYI r'GATC_y
Cuts at: 1972 2656 3082 4361 4570 6469 6836 7221 7238
Size: 684 426 1279 209 1899 367 385 17
Cuts at: 7238 8690 8776 8788 9134 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1899 1452 1279 684 426 385 367
346 209 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3559 3559
Size: 9139
BtgI C'CryG_G
Cuts at: 513 1065 2004 3670 6122 513
Size: 552 939 1666 2452 3530
Fragments arranged by size:
3530 2452 1666 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4539 4917 487
Size: 683 56 3313 378 4709
Fragments arranged by size:
4709 3313 683 378 56
BtsI GCAGTG_nn'
Cuts at: 3171 3715 5276 6194 6655 7449 7469 3171
Size: 544 1561 918 461 794 20 4841
Fragments arranged by size:
4841 1561 918 794 544 461 20
ClaI AT'CG_AT
Cuts at: 1554 3433 5743 6426 1554
Size: 1879 2310 683 4267
Fragments arranged by size:
4267 2310 1879 683
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2760 2795 6297 6332 529
Size: 35 2196 35 3502 35 3336
Fragments arranged by size:
3502 3336 2196 35 35 35
DraI TTT'AAA
Cuts at: 2551 6443 2551
Size: 3892 5247
DraIII CAC_nnn'GTG
Cuts at: 2384 2384
Size: 9139
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8146 809
Size: 507 6830 1802
Fragments arranged by size:
6830 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3342 4479 5736
Size: 54 1425 50 275 18 1512 1137 1257
Cuts at: 5736 5949 6125 6234 6586 7475 9104 8
Size: 213 176 109 352 889 1629 43
Fragments arranged by size:
1629 1512 1425 1257 1137 889 352 275
213 176 109 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6586 1537
Size: 275 4774 4090
Fragments arranged by size:
4774 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3069 3263 3534 4773 6093 7069 1353
Size: 1716 194 271 1239 1320 976 3423
Fragments arranged by size:
3423 1716 1320 1239 976 271 194
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7726 8100 8246 387
Size: 1064 138 6137 374 146 1280
Fragments arranged by size:
6137 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4710 6086 4710
Size: 1376 7763
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2214 2373 2438 2521 2583 3093 3303
Size: 393 582 159 65 83 62 510 210
Cuts at: 3303 3387 3399 3567 3753 3988 4238 4629 4779
Size: 84 12 168 186 235 250 391 150
Cuts at: 4779 4905 5526 6074 6276 6387 7202 7799 8586
Size: 126 621 548 202 111 815 597 787
Cuts at: 8586 8854 1239
Size: 268 1524
Fragments arranged by size:
1524 815 787 621 597 582 548 510
393 391 268 250 235 210 202 186
168 159 150 126 111 84 83 65
62 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2045 2453 4193 2045
Size: 408 1740 6991
Fragments arranged by size:
6991 1740 408
EcoO109I rG'GnC_Cy
Cuts at: 3569 3607 3737 3809 4180 5024 3569
Size: 38 130 72 371 844 7684
Fragments arranged by size:
7684 844 371 130 72 38
EcoRI G'AATT_C
Cuts at: 2297 5804 6410 6448 6826 2297
Size: 3507 606 38 378 4610
Fragments arranged by size:
4610 3507 606 378 38
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4161 4695 5948 6007 4161
Size: 534 1253 59 7293
Fragments arranged by size:
7293 1253 534 59
FspI TGC'GCA
Cuts at: 7645 7645
Size: 9139
HaeII r_GCGC'y
Cuts at: 1678 8286 1678
Size: 6608 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2305 2337 2842 2874 3776
Size: 32 135 32 733 32 505 32 902
Cuts at: 3776 3808 4346 4378 4438 4470 4481 4513 4706
Size: 32 538 32 60 32 11 32 193
Cuts at: 4706 4738 4898 4930 5021 5053 6061 6093 7780
Size: 32 160 32 91 32 1008 32 1687
Cuts at: 7780 7812 7854 7886 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1687 1008 902 733 538 505 193
160 135 91 60 42 32 32 32
32 32 32 32 32 32 32 32
32 32 32 11
HincII GTy'rAC
Cuts at: 669 1657 1938 5666 6483 6729 669
Size: 988 281 3728 817 246 3079
Fragments arranged by size:
3728 3079 988 817 281 246
HindIII A'AGCT_T
Cuts at: 748 2196 4143 748
Size: 1448 1947 5744
Fragments arranged by size:
5744 1947 1448
HpaI GTT'AAC
Cuts at: 6729 6729
Size: 9139
KpnI G_GTAC'C
Cuts at: 6464 6464
Size: 9139
MfeI C'AATT_G
Cuts at: 2267 2419 6738 2267
Size: 152 4319 4668
Fragments arranged by size:
4668 4319 152
MluI A'CGCG_T
Cuts at: 9015 9015
Size: 9139
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 8253 8437 1305
Size: 6948 184 2007
Fragments arranged by size:
6948 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4481 5738 5951 6127 6236
Size: 54 1425 343 2649 1257 213 176 109
Cuts at: 6236 10
Size: 2913
Fragments arranged by size:
2913 2649 1425 1257 343 213 176 109
54
MslI CAynn'nnrTG
Cuts at: 518 1166 3470 7097 7456 7615 518
Size: 648 2304 3627 359 159 2042
Fragments arranged by size:
3627 2304 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2117 3844 4444 4649 4755 4799 5837 5947
Size: 511 1727 600 205 106 44 1038 110
Cuts at: 5947 6555 7232 8380 8625 1606
Size: 608 677 1148 245 2120
Fragments arranged by size:
2120 1727 1148 1038 677 608 600 511
245 205 110 106 44
NcoI C'CATG_G
Cuts at: 513 1065 2004 3670 6122 513
Size: 552 939 1666 2452 3530
Fragments arranged by size:
3530 2452 1666 939 552
NdeI CA'TA_TG
Cuts at: 387 3489 5906 387
Size: 3102 2417 3620
Fragments arranged by size:
3620 3102 2417
NheI G'CTAG_C
Cuts at: 1052 6353 1052
Size: 5301 3838
NotI GC'GGCC_GC
Cuts at: 6586 6586
Size: 9139
NruI TCG'CGA
Cuts at: 6024 6024
Size: 9139
NspI r_CATG'y
Cuts at: 1132 1229 2225 3619 4057 6497 8042 1132
Size: 97 996 1394 438 2440 1545 2229
Fragments arranged by size:
2440 2229 1545 1394 996 438 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9139
PciI A'CATG_T
Cuts at: 4053 8038 4053
Size: 3985 5154
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4625 1108
Size: 3517 5622
PfoI T'CCnGG_A
Cuts at: 3522 4581 4768 3522
Size: 1059 187 7893
Fragments arranged by size:
7893 1059 187
PmeI GTTT'AAAC
Cuts at: 6443 6443
Size: 9139
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7208 7240 8721 8753 7208
Size: 32 1481 32 7594
Fragments arranged by size:
7594 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3569 3607 3737 3809 3569
Size: 38 130 72 8899
Fragments arranged by size:
8899 130 72 38
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9139
PsiI TTA'TAA
Cuts at: 6165 6709 6165
Size: 544 8595
PstI C_TGCA'G
Cuts at: 830 2959 3710 4045 4804 5283 5874 6491 830
Size: 2129 751 335 759 479 591 617 3478
Fragments arranged by size:
3478 2129 759 751 617 591 479 335
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9139
PvuII CAG'CTG
Cuts at: 2117 3844 4444 4649 4755 4799 5837 5947 2117
Size: 1727 600 205 106 44 1038 110 5309
Fragments arranged by size:
5309 1727 1038 600 205 110 106 44
SacI G_AGCT'C
Cuts at: 4712 6088 4712
Size: 1376 7763
SalI G'TCGA_C
Cuts at: 1936 5664 6481 1936
Size: 3728 817 4594
Fragments arranged by size:
4594 3728 817
SapI GCTCTTCn'nnn_
Cuts at: 4773 6093 4773
Size: 1320 7819
SbfI CC_TGCA'GG
Cuts at: 6491 6491
Size: 9139
ScaI AGT'ACT
Cuts at: 7387 9099 7387
Size: 1712 7427
SexAI A'CCwGG_T
Cuts at: 6027 6027
Size: 9139
SfcI C'TryA_G
Cuts at: 826 1045 2955 3235 3441 3706 3839 4041 4800
Size: 219 1910 280 206 265 133 202 759
Cuts at: 4800 5279 5623 5675 5870 6487 7622 8303 8494
Size: 479 344 52 195 617 1135 681 191
Cuts at: 8494 826
Size: 1471
Fragments arranged by size:
1910 1471 1135 759 681 617 479 344
280 265 219 206 202 195 191 133
52
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9139
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9139
SmaI CCC'GGG
Cuts at: 1872 6176 6466 1872
Size: 4304 290 4545
Fragments arranged by size:
4545 4304 290
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2042 2108 2302 3510 4870 5777
Size: 197 926 99 66 194 1208 1360 907
Cuts at: 5777 7243 8144 8406 820
Size: 1466 901 262 1553
Fragments arranged by size:
1553 1466 1360 1208 926 907 901 262
197 194 99 66
SnaBI TAC'GTA
Cuts at: 493 6930 493
Size: 6437 2702
SpeI A'CTAG_T
Cuts at: 152 4403 4693 152
Size: 4251 290 4598
Fragments arranged by size:
4598 4251 290
SphI G_CATG'C
Cuts at: 1132 6497 1132
Size: 5365 3774
SspI AAT'ATT
Cuts at: 5 52 4006 7063 5
Size: 47 3954 3057 2081
Fragments arranged by size:
3954 3057 2081 47
StuI AGG'CCT
Cuts at: 1517 4296 1517
Size: 2779 6360
StyI C'CwwG_G
Cuts at: 513 1065 2004 2504 3103 3670 4931 6122 6578
Size: 552 939 500 599 567 1261 1191 456
Cuts at: 6578 513
Size: 3074
Fragments arranged by size:
3074 1261 1191 939 599 567 552 500
456
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5225 6447 7166 7183 7336 7521 7966
Size: 1255 3645 1222 719 17 153 185 445
Cuts at: 7966 325
Size: 1498
Fragments arranged by size:
3645 1498 1255 1222 719 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2104 2865 2921
Size: 274 80 33 51 1443 127 761 56
Cuts at: 2921 3971 5168 7385 9097 96
Size: 1050 1197 2217 1712 138
Fragments arranged by size:
2217 1712 1443 1197 1050 761 274 138
127 80 56 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3774 3806 5038 5070 6142 6174 9099 9131 3774
Size: 32 1232 32 1072 32 2925 32 3782
Fragments arranged by size:
3782 2925 1232 1072 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 6248 1316
Size: 4932 4207
XbaI T'CTAG_A
Cuts at: 6475 6475
Size: 9139
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3475 3475
Size: 9139
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9139
XmaI C'CCGG_G
Cuts at: 1870 6174 6464 1870
Size: 4304 290 4545
Fragments arranged by size:
4545 4304 290
XmnI GAAnn'nnTTC
Cuts at: 2986 5808 5865 5931 7268 2986
Size: 2822 57 66 1337 4857
Fragments arranged by size:
4857 2822 1337 66 57
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3979 6947 276
Size: 53 83 186 936 2445 2968 2468
Fragments arranged by size:
2968 2468 2445 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NruI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BbeI BfrBI BsiWI BsmBI
BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII
SanDI SfiI SfoI SrfI SwaI