Restriction map for
pFN21AA1520
(Circular) (Six-base) MAPSORT of: pFN21AA1520.seq Check: 8339 from: 1 to: 7003
pFN21AA1520 7003 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3184 3184
Size: 7003
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4813 278
Size: 53 83 186 936 3277 2468
Fragments arranged by size:
3277 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3399 4346 1937
Size: 1462 947 4594
Fragments arranged by size:
4594 1462 947
Acc65I G'GTAC_C
Cuts at: 4324 4324
Size: 7003
AclI AA'CG_TT
Cuts at: 1563 5130 5503 1563
Size: 3567 373 3063
Fragments arranged by size:
3567 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2068 3568 4223 5066 6450 6718 1632
Size: 436 1500 655 843 1384 268 1917
Fragments arranged by size:
1917 1500 1384 843 655 436 268
AfeI AGC'GCT
Cuts at: 4018 4018
Size: 7003
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6806
AflIII A'CryG_T
Cuts at: 1203 3231 5902 6879 1203
Size: 2028 2671 977 1327
Fragments arranged by size:
2671 2028 1327 977
AgeI A'CCGG_T
Cuts at: 5834 5834
Size: 7003
AhdI GACnn_n'nnGTC
Cuts at: 5732 5732
Size: 7003
AleI CACnn'nnGTG
Cuts at: 1166 2267 2951 1166
Size: 1101 684 5218
Fragments arranged by size:
5218 1101 684
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2198 1184
Size: 1014 5989
AlwNI CAG_nnn'CTG
Cuts at: 2443 2533 3568 4183 4186 4231 4746 6318 2443
Size: 90 1035 615 3 45 515 1572 3128
Fragments arranged by size:
3128 1572 1035 615 515 90 45 3
ApaI G_GGCC'C
Cuts at: 2232 2359 3624 2232
Size: 127 1265 5611
Fragments arranged by size:
5611 1265 127
ApaLI G'TGCA_C
Cuts at: 3473 5060 6216 3473
Size: 1587 1156 4260
Fragments arranged by size:
4260 1587 1156
ApoI r'AATT_y
Cuts at: 1493 4312 4538 4690 6799 1493
Size: 2819 226 152 2109 1697
Fragments arranged by size:
2819 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5557 160
Size: 872 4525 1606
Fragments arranged by size:
4525 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7003
AvaI C'yCGr_G
Cuts at: 1870 1943 2501 3625 3966 4328 1870
Size: 73 558 1124 341 362 4545
Fragments arranged by size:
4545 1124 558 362 341 73
BamHI G'GATC_C
Cuts at: 4333 4700 4333
Size: 367 6636
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2432 3923 4169 4324 5779
Size: 325 217 627 645 1491 246 155 1455
Cuts at: 5779 618
Size: 1842
Fragments arranged by size:
1842 1491 1455 645 627 325 246 217
155
BanII G_rGCy'C
Cuts at: 1391 2232 2359 3258 3395 3624 3644 3771 3973
Size: 841 127 899 137 229 20 127 202
Cuts at: 3973 4322 1391
Size: 349 4072
Fragments arranged by size:
4072 899 841 349 229 202 137 127
127 20
BbeI G_GCGC'C
Cuts at: 3927 3927
Size: 7003
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2074 2914 2953 3137 928
Size: 971 175 840 39 184 4794
Fragments arranged by size:
4794 971 840 184 175 39
BbvCI CC'TCA_GC
Cuts at: 2469 2469
Size: 7003
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2671 2705 3322 3356 3454
Size: 34 50 34 650 34 617 34 98
Cuts at: 3454 3488 5194 5228 1903
Size: 34 1706 34 3678
Fragments arranged by size:
3678 1706 650 617 98 50 34 34
34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4895 6105 4895
Size: 1210 5793
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7003
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2236 2465 5614
Size: 107 122 71 631 605 564 229 3149
Cuts at: 5614 136
Size: 1525
Fragments arranged by size:
3149 1525 631 605 564 229 122 107
71
BglII A'GATC_T
Cuts at: 6998 6998
Size: 7003
BlpI GC'TnA_GC
Cuts at: 4106 4420 4106
Size: 314 6689
Bme1580I G_kGCm'C
Cuts at: 1790 2232 2359 3477 3624 4172 5064 6220 1790
Size: 442 127 1118 147 548 892 1156 2573
Fragments arranged by size:
2573 1156 1118 892 548 442 147 127
BmgBI CAC'GTC
Cuts at: 1318 2557 1318
Size: 1239 5764
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3983 4734 5692
Size: 416 53 83 399 24 2566 751 958
Cuts at: 5692 442
Size: 1753
Fragments arranged by size:
2566 1753 958 751 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7003
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2129 2465 3524 3680 3698 3872 5663 1239
Size: 890 336 1059 156 18 174 1791 2579
Fragments arranged by size:
2579 1791 1059 890 336 174 156 18
Bpu10I CC'TnA_GC
Cuts at: 2469 2469
Size: 7003
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5128 5993 6291 5128
Size: 865 298 5840
Fragments arranged by size:
5840 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2603 2908 3107 5666 882
Size: 617 1104 305 199 2559 2219
Fragments arranged by size:
2559 2219 1104 617 305 199
BsaAI yAC'GTr
Cuts at: 493 1204 4794 493
Size: 711 3590 2702
Fragments arranged by size:
3590 2702 711
BsaBI GATnn'nnATC
Cuts at: 6997 6997
Size: 7003
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2510 3924 4810 5192
Size: 53 83 186 936 977 1414 886 382
Cuts at: 5192 5827 275
Size: 635 1451
Fragments arranged by size:
1451 1414 977 936 886 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3138 5436 5834 6108 6255 6666 3138
Size: 2298 398 274 147 411 3475
Fragments arranged by size:
3475 2298 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2846 2876 2888 2918 1178
Size: 30 165 30 1443 30 12 30 5263
Fragments arranged by size:
5263 1443 165 30 30 30 30 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3125 3170 3173 3176 1186
Size: 1939 45 3 3 5013
Fragments arranged by size:
5013 1939 45 3 3
BseYI C'CCAG_C
Cuts at: 2188 2224 3927 4231 6206 6702 2188
Size: 36 1703 304 1975 496 2489
Fragments arranged by size:
2489 1975 1703 496 304 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4181 4238 1163
Size: 573 2445 57 3928
Fragments arranged by size:
3928 2445 573 57
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2491 3457 4453 5214 6242 1540
Size: 275 186 490 966 996 761 1028 2301
Fragments arranged by size:
2301 1028 996 966 761 490 275 186
BsiHKAI G_wGCw'C
Cuts at: 3477 3644 4112 4122 5064 5149 6220 3477
Size: 167 468 10 942 85 1071 4260
Fragments arranged by size:
4260 1071 942 468 167 85 10
BsmI GAATG_Cn'
Cuts at: 3085 4514 4607 3085
Size: 1429 93 5481
Fragments arranged by size:
5481 1429 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2232 2359 3258 3395 3477 3624 3644
Size: 399 442 127 899 137 82 147 20
Cuts at: 3644 3771 3973 4112 4122 4172 4322 5064 5149
Size: 127 202 139 10 50 150 742 85
Cuts at: 5149 6220 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 899 742 442 399 202 150
147 139 137 127 127 85 82 50
20 10
BspEI T'CCGG_A
Cuts at: 3138 6666 3138
Size: 3528 3475
BspHI T'CATG_A
Cuts at: 2043 3021 4892 6622 2043
Size: 978 1871 1730 2424
Fragments arranged by size:
2424 1871 1730 978
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2723 3184 3186 4077 4358 844
Size: 1879 461 2 891 281 3489
Fragments arranged by size:
3489 1879 891 461 281 2
BsrBI CCG'CTC
Cuts at: 1394 1823 2487 2805 3786 4890 1394
Size: 429 664 318 981 1104 3507
Fragments arranged by size:
3507 1104 981 664 429 318
BsrDI GCAATG_nn'
Cuts at: 65 1248 5498 5672 65
Size: 1183 4250 174 1396
Fragments arranged by size:
4250 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2490 3456 4101 5647 5834 1700
Size: 110 680 966 645 1546 187 2869
Fragments arranged by size:
2869 1546 966 680 645 187 110
BsrGI T'GTAC_A
Cuts at: 96 3198 96
Size: 3102 3901
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7003
BssSI C'ACGA_G
Cuts at: 2248 2267 2567 5063 6075 2248
Size: 19 300 2496 1012 3176
Fragments arranged by size:
3176 2496 1012 300 19
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2533 794
Size: 1739 5264
BstBI TT'CG_AA
Cuts at: 2788 5807 2788
Size: 3019 3984
BstEII G'GTnAC_C
Cuts at: 1184 2995 1184
Size: 1811 5192
BstXI CCAn_nnnn'nTGG
Cuts at: 2206 4021 2206
Size: 1815 5188
BstYI r'GATC_y
Cuts at: 1972 2149 4333 4700 5085 5102 6554 6640 6652
Size: 177 2184 367 385 17 1452 86 12
Cuts at: 6652 6998 1972
Size: 346 1977
Fragments arranged by size:
2184 1977 1452 385 367 346 177 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2067 2295 2886 3387 3468 3868 3960
Size: 552 1002 228 591 501 81 400 92
Cuts at: 3960 4060 4113 513
Size: 100 53 3403
Fragments arranged by size:
3403 1002 591 552 501 400 228 100
92 81 53
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3325 4078 487
Size: 683 56 2099 753 3412
Fragments arranged by size:
3412 2099 753 683 56
BtsI GCAGTG_nn'
Cuts at: 3260 3637 4519 5313 5333 3260
Size: 377 882 794 20 4930
Fragments arranged by size:
4930 882 794 377 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7003
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6968
DraI TTT'AAA
Cuts at: 4307 4307
Size: 7003
DraIII CAC_nnn'GTG
Cuts at: 3745 3745
Size: 7003
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6010 809
Size: 507 4694 1802
Fragments arranged by size:
4694 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2070 2208 2426
Size: 54 1425 50 275 18 240 138 218
Cuts at: 2426 2457 2488 2492 2571 2709 3087 3940 3957
Size: 31 31 4 79 138 378 853 17
Cuts at: 3957 4264 4450 5339 6968 8
Size: 307 186 889 1629 43
Fragments arranged by size:
1629 1425 889 853 378 307 275 240
218 186 138 138 79 54 50 43
31 31 18 17 4
EagI C'GGCC_G
Cuts at: 1537 1812 2488 4450 1537
Size: 275 676 1962 4090
Fragments arranged by size:
4090 1962 676 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2282 2318 2804 3632 4933 1353
Size: 929 36 486 828 1301 3423
Fragments arranged by size:
3423 1301 929 828 486 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2108 3122 5590 5964 6110 387
Size: 1064 138 519 1014 2468 374 146 1280
Fragments arranged by size:
2468 1280 1064 1014 519 374 146 138
EcoICRI GAG'CTC
Cuts at: 3642 3642
Size: 7003
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2068 2129 2465 3524 3568 3680 3698
Size: 393 436 61 336 1059 44 112 18
Cuts at: 3698 3872 4223 5066 5663 6450 6718 1239
Size: 174 351 843 597 787 268 1524
Fragments arranged by size:
1524 1059 843 787 597 436 393 351
336 268 174 112 61 44 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2108 3413 2108
Size: 1305 5698
EcoO109I rG'GnC_Cy
Cuts at: 2228 2229 2341 2356 2440 2854 3565 3620 3621
Size: 1 112 15 84 414 711 55 1
Cuts at: 3621 2228
Size: 5610
Fragments arranged by size:
5610 711 414 112 84 55 15 1
1
EcoRI G'AATT_C
Cuts at: 4312 4690 4312
Size: 378 6625
FseI GG_CCGG'CC
Cuts at: 2494 2494
Size: 7003
FspI TGC'GCA
Cuts at: 2327 5509 2327
Size: 3182 3821
HaeII r_GCGC'y
Cuts at: 1678 3720 3927 4020 6150 1678
Size: 2042 207 93 2130 2531
Fragments arranged by size:
2531 2130 2042 207 93
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2495 2527 2846 2878 2888
Size: 32 135 32 923 32 319 32 10
Cuts at: 2888 2920 3788 3820 4024 4056 5644 5676 5718
Size: 32 868 32 204 32 1588 32 42
Cuts at: 5718 5750 1373
Size: 32 2626
Fragments arranged by size:
2626 1588 923 868 319 204 135 42
32 32 32 32 32 32 32 32
32 10
HincII GTy'rAC
Cuts at: 669 1657 1938 3652 4347 4593 669
Size: 988 281 1714 695 246 3079
Fragments arranged by size:
3079 1714 988 695 281 246
HindIII A'AGCT_T
Cuts at: 748 2827 748
Size: 2079 4924
HpaI GTT'AAC
Cuts at: 4593 4593
Size: 7003
KasI G'GCGC_C
Cuts at: 3923 3923
Size: 7003
KpnI G_GTAC'C
Cuts at: 4328 4328
Size: 7003
MfeI C'AATT_G
Cuts at: 4602 4602
Size: 7003
MluI A'CGCG_T
Cuts at: 6879 6879
Size: 7003
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3057 6117 6301 1305
Size: 1752 3060 184 2007
Fragments arranged by size:
3060 2007 1752 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2210 2711 3089 3942 3959
Size: 54 1425 343 378 501 378 853 17
Cuts at: 3959 4266 10
Size: 307 2747
Fragments arranged by size:
2747 1425 853 501 378 378 343 307
54 17
MslI CAynn'nnrTG
Cuts at: 518 1166 2048 2060 2204 2267 2885 2951 3002
Size: 648 882 12 144 63 618 66 51
Cuts at: 3002 3020 3023 3026 4136 4961 5320 5479 518
Size: 18 3 3 1110 825 359 159 2042
Fragments arranged by size:
2042 1110 882 825 648 618 359 159
144 66 63 51 18 12 3 3
MspA1I CmG'CkG
Cuts at: 1606 2178 3224 3252 3330 3931 3952 4183 4222
Size: 572 1046 28 78 601 21 231 39
Cuts at: 4222 4262 4419 5096 6244 6489 1606
Size: 40 157 677 1148 245 2120
Fragments arranged by size:
2120 1148 1046 677 601 572 245 231
157 78 40 39 28 21
NaeI GCC'GGC
Cuts at: 2492 2492
Size: 7003
NarI GG'CG_CC
Cuts at: 3924 3924
Size: 7003
NcoI C'CATG_G
Cuts at: 513 1065 2295 2886 3468 3960 4060 513
Size: 552 1230 591 582 492 100 3456
Fragments arranged by size:
3456 1230 591 582 552 492 100
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7003
NgoMIV G'CCGG_C
Cuts at: 2490 2490
Size: 7003
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7003
NotI GC'GGCC_GC
Cuts at: 4450 4450
Size: 7003
NspI r_CATG'y
Cuts at: 1132 1229 3235 4361 5906 1132
Size: 97 2006 1126 1545 2229
Fragments arranged by size:
2229 2006 1545 1126 97
PasI CC'CwG_GG
Cuts at: 1348 2480 4192 1348
Size: 1132 1712 4159
Fragments arranged by size:
4159 1712 1132
PciI A'CATG_T
Cuts at: 3231 5902 3231
Size: 2671 4332
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2443 1108
Size: 1335 5668
PfoI T'CCnGG_A
Cuts at: 2106 2337 3657 3888 3999 2106
Size: 231 1320 231 111 5110
Fragments arranged by size:
5110 1320 231 231 111
PmeI GTTT'AAAC
Cuts at: 4307 4307
Size: 7003
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3425 3457 5072 5104 6585 6617 3425
Size: 32 1615 32 1481 32 3811
Fragments arranged by size:
3811 1615 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2341 2854 3565 2341
Size: 513 711 5779
Fragments arranged by size:
5779 711 513
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7003
PsiI TTA'TAA
Cuts at: 4573 4573
Size: 7003
PspOMI G'GGCC_C
Cuts at: 2228 2355 3620 2228
Size: 127 1265 5611
Fragments arranged by size:
5611 1265 127
PstI C_TGCA'G
Cuts at: 830 2529 3192 3573 4074 4355 830
Size: 1699 663 381 501 281 3478
Fragments arranged by size:
3478 1699 663 501 381 281
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7003
PvuII CAG'CTG
Cuts at: 2178 3224 3931 4183 4262 2178
Size: 1046 707 252 79 4919
Fragments arranged by size:
4919 1046 707 252 79
SacI G_AGCT'C
Cuts at: 3644 3644
Size: 7003
SalI G'TCGA_C
Cuts at: 1936 4345 1936
Size: 2409 4594
SanDI GG'GwC_CC
Cuts at: 2341 2341
Size: 7003
SapI GCTCTTCn'nnn_
Cuts at: 2318 3632 2318
Size: 1314 5689
SbfI CC_TGCA'GG
Cuts at: 4355 4355
Size: 7003
ScaI AGT'ACT
Cuts at: 3050 3734 5251 6963 3050
Size: 684 1517 1712 3090
Fragments arranged by size:
3090 1712 1517 684
SexAI A'CCwGG_T
Cuts at: 2898 2898
Size: 7003
SfcI C'TryA_G
Cuts at: 826 1045 2525 3188 3400 3569 3898 4070 4278
Size: 219 1480 663 212 169 329 172 208
Cuts at: 4278 4351 5486 6167 6358 826
Size: 73 1135 681 191 1471
Fragments arranged by size:
1480 1471 1135 681 663 329 219 212
208 191 172 169 73
SfoI GGC'GCC
Cuts at: 3925 3925
Size: 7003
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7003
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7003
SmaI CCC'GGG
Cuts at: 1872 3968 4330 1872
Size: 2096 362 4545
Fragments arranged by size:
4545 2096 362
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5107 6008 6270 820
Size: 197 926 3164 901 262 1553
Fragments arranged by size:
3164 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4794 493
Size: 4301 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7003
SphI G_CATG'C
Cuts at: 1132 4361 1132
Size: 3229 3774
SrfI GCCC'GGGC
Cuts at: 3968 3968
Size: 7003
SspI AAT'ATT
Cuts at: 5 52 4927 5
Size: 47 4875 2081
Fragments arranged by size:
4875 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7003
StyI C'CwwG_G
Cuts at: 513 1065 2295 2886 2991 3468 3960 4060 4442
Size: 552 1230 591 105 477 492 100 382
Cuts at: 4442 513
Size: 3074
Fragments arranged by size:
3074 1230 591 552 492 477 382 105
100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2450 3575 4187 5030 5047 5200 5385
Size: 1255 870 1125 612 843 17 153 185
Cuts at: 5385 5830 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1125 870 843 612 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3048 3198 3234
Size: 274 80 33 51 1443 1071 150 36
Cuts at: 3234 3732 5249 6961 96
Size: 498 1517 1712 138
Fragments arranged by size:
1712 1517 1443 1071 498 274 150 138
80 51 36 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6963 6995 6963
Size: 32 6971
Tth111I GACn'n_nGTC
Cuts at: 1316 2900 1316
Size: 1584 5419
XbaI T'CTAG_A
Cuts at: 4339 4339
Size: 7003
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2203 2554 3022 3935 4018 2203
Size: 351 468 913 83 5188
Fragments arranged by size:
5188 913 468 351 83
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7003
XmaI C'CCGG_G
Cuts at: 1870 3966 4328 1870
Size: 2096 362 4545
Fragments arranged by size:
4545 2096 362
XmnI GAAnn'nnTTC
Cuts at: 3440 5132 3440
Size: 1692 5311
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4811 276
Size: 53 83 186 936 3277 2468
Fragments arranged by size:
3277 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI
BstZ17I Bsu36I EcoRV FalI FspAI NruI NsiI PacI
PmlI PspXI PsrI RsrII SacII SfiI SwaI