Restriction map for
pFN21AA1516
(Circular) (Six-base) MAPSORT of: pFN21AA1516.seq Check: 1996 from: 1 to: 11611
pFN21AA1516 11611 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 8707 8707
Size: 11611
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5498 7692 9421 278
Size: 53 83 186 936 3962 2194 1729 2468
Fragments arranged by size:
3962 2468 2194 1729 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3026 3663 4836 7686 8954 1937
Size: 1089 637 1173 2850 1268 4594
Fragments arranged by size:
4594 2850 1268 1173 1089 637
Acc65I G'GTAC_C
Cuts at: 8932 8932
Size: 11611
AclI AA'CG_TT
Cuts at: 1563 9738 10111 1563
Size: 8175 373 3063
Fragments arranged by size:
8175 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2034 4046 4154 4640 4922 4984 5723 5789
Size: 402 2012 108 486 282 62 739 66
Cuts at: 5789 5969 6833 7886 9674 11058 11326 1632
Size: 180 864 1053 1788 1384 268 1917
Fragments arranged by size:
2012 1917 1788 1384 1053 864 739 486
402 282 268 180 108 66 62
AflII C'TTAA_G
Cuts at: 820 1017 8702 820
Size: 197 7685 3729
Fragments arranged by size:
7685 3729 197
AflIII A'CryG_T
Cuts at: 1203 4079 10510 11487 1203
Size: 2876 6431 977 1327
Fragments arranged by size:
6431 2876 1327 977
AgeI A'CCGG_T
Cuts at: 3404 10442 3404
Size: 7038 4573
AhdI GACnn_n'nnGTC
Cuts at: 10340 10340
Size: 11611
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 11611
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3671 5749 1184
Size: 2487 2078 7046
Fragments arranged by size:
7046 2487 2078
AlwNI CAG_nnn'CTG
Cuts at: 2030 2297 4585 4913 5071 7330 7357 7777 8128
Size: 267 2288 328 158 2259 27 420 351
Cuts at: 8128 9354 10926 2030
Size: 1226 1572 2715
Fragments arranged by size:
2715 2288 2259 1572 1226 420 351 328
267 158 27
ApaLI G'TGCA_C
Cuts at: 4574 9668 10824 4574
Size: 5094 1156 5361
Fragments arranged by size:
5361 5094 1156
ApoI r'AATT_y
Cuts at: 1493 2421 2457 4282 4760 5045 5654 6139 6501
Size: 928 36 1825 478 285 609 485 362
Cuts at: 6501 6539 6947 7031 7975 8136 8280 8693 8920
Size: 38 408 84 944 161 144 413 227
Cuts at: 8920 9146 9298 11407 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1825 1697 944 928 609 485 478
413 408 362 285 227 226 161 152
144 84 38 36
AseI AT'TA_AT
Cuts at: 160 1032 5382 8100 10165 160
Size: 872 4350 2718 2065 1606
Fragments arranged by size:
4350 2718 2065 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11611
AvaI C'yCGr_G
Cuts at: 1870 1943 8730 8802 8936 1870
Size: 73 6787 72 134 4545
Fragments arranged by size:
6787 4545 134 73 72
BamHI G'GATC_C
Cuts at: 3470 7603 8941 9308 3470
Size: 4133 1338 367 5773
Fragments arranged by size:
5773 4133 1338 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4160 4972 8108 8932 10387
Size: 325 217 627 2373 812 3136 824 1455
Cuts at: 10387 618
Size: 1842
Fragments arranged by size:
3136 2373 1842 1455 824 812 627 325
217
BanII G_rGCy'C
Cuts at: 1391 3716 3821 4143 4539 5452 5552 7133 8082
Size: 2325 105 322 396 913 100 1581 949
Cuts at: 8082 8399 8930 1391
Size: 317 531 4072
Fragments arranged by size:
4072 2325 1581 949 913 531 396 322
317 105 100
BbeI G_GCGC'C
Cuts at: 4164 4164
Size: 11611
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2406 2946 5526 6819 8612 928
Size: 971 507 540 2580 1293 1793 3927
Fragments arranged by size:
3927 2580 1793 1293 971 540 507
BbvCI CC'TCA_GC
Cuts at: 2394 6044 6480 2394
Size: 3650 436 7525
Fragments arranged by size:
7525 3650 436
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6284 6318 7666 7700 8711 8740 8745
Size: 34 4347 34 1348 34 1011 29 5
Cuts at: 8745 8774 9802 9836 1903
Size: 29 1028 34 3678
Fragments arranged by size:
4347 3678 1348 1028 1011 34 34 34
34 29 29 5
BciVI GTATCCnnnnn_n'
Cuts at: 3216 7422 8303 8855 9503 10713 3216
Size: 4206 881 552 648 1210 4114
Fragments arranged by size:
4206 4114 1210 881 648 552
BclI T'GATC_A
Cuts at: 1548 2517 2764 6958 1548
Size: 969 247 4194 6201
Fragments arranged by size:
6201 4194 969 247
BfrBI ATG'CAT
Cuts at: 7058 7058
Size: 11611
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2075 3507 4851
Size: 107 122 71 631 605 403 1432 1344
Cuts at: 4851 10222 136
Size: 5371 1525
Fragments arranged by size:
5371 1525 1432 1344 631 605 403 122
107 71
BglII A'GATC_T
Cuts at: 3790 5818 7588 7798 11606 3790
Size: 2028 1770 210 3808 3795
Fragments arranged by size:
3808 3795 2028 1770 210
BlpI GC'TnA_GC
Cuts at: 4749 9028 4749
Size: 4279 7332
Bme1580I G_kGCm'C
Cuts at: 1790 3698 4087 4578 5733 7652 9672 10828 1790
Size: 1908 389 491 1155 1919 2020 1156 2573
Fragments arranged by size:
2573 2020 1919 1908 1156 1155 491 389
BmgBI CAC'GTC
Cuts at: 1318 3429 7233 1318
Size: 2111 3804 5696
Fragments arranged by size:
5696 3804 2111
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2065 3193 4365
Size: 416 53 83 399 24 648 1128 1172
Cuts at: 4365 4781 4898 5949 6947 7647 9342 10300 442
Size: 416 117 1051 998 700 1695 958 1753
Fragments arranged by size:
1753 1695 1172 1128 1051 998 958 700
648 416 416 399 117 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 11611
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3835 5973 3835
Size: 2138 9473
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4367 4372 4634 7515 8424 8869 10271 1239
Size: 3128 5 262 2881 909 445 1402 2579
Fragments arranged by size:
3128 2881 2579 1402 909 445 262 5
Bpu10I CC'TnA_GC
Cuts at: 2394 6044 6480 8540 2394
Size: 3650 436 2060 5465
Fragments arranged by size:
5465 3650 2060 436
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5358 8413 8756 9736 10601 10899 5358
Size: 3055 343 980 865 298 6070
Fragments arranged by size:
6070 3055 980 865 343 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2107 4004 4175 10274 882
Size: 617 608 1897 171 6099 2219
Fragments arranged by size:
6099 2219 1897 617 608 171
BsaAI yAC'GTr
Cuts at: 493 1204 4082 4552 9402 493
Size: 711 2878 470 4850 2702
Fragments arranged by size:
4850 2878 2702 711 470
BsaBI GATnn'nnATC
Cuts at: 6347 11605 6347
Size: 5258 6353
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4161 5495 6644 7689
Size: 53 83 186 936 2628 1334 1149 1045
Cuts at: 7689 9418 9800 10435 275
Size: 1729 382 635 1451
Fragments arranged by size:
2628 1729 1451 1334 1149 1045 936 635
382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3404 4166 4326 10044 10442 10716 10863 11274 3404
Size: 762 160 5718 398 274 147 411 3741
Fragments arranged by size:
5718 3741 762 411 398 274 160 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2977 3007 3169 3199 4148
Size: 30 165 30 1574 30 162 30 949
Cuts at: 4148 4178 4280 4310 5485 5515 5557 5587 8642
Size: 30 102 30 1175 30 42 30 3055
Cuts at: 8642 8672 1178
Size: 30 4117
Fragments arranged by size:
4117 3055 1574 1175 949 165 162 102
42 30 30 30 30 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2687 3875 4033 4373 6773 8282 8417 8420
Size: 1501 1188 158 340 2400 1509 135 3
Cuts at: 8420 8873 1186
Size: 453 3924
Fragments arranged by size:
3924 2400 1509 1501 1188 453 340 158
135 3
BseYI C'CCAG_C
Cuts at: 3283 3551 4041 4705 5180 5468 6324 7335 8337
Size: 268 490 664 475 288 856 1011 1002
Cuts at: 8337 10814 11310 3283
Size: 2477 496 3584
Fragments arranged by size:
3584 2477 1011 1002 856 664 496 490
475 288 268
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4073 6010 6716 8469 8706 8741 1163
Size: 573 2337 1937 706 1753 237 35 4033
Fragments arranged by size:
4033 2337 1937 1753 706 573 237 35
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 9061 9822 10850 1540
Size: 275 186 7060 761 1028 2301
Fragments arranged by size:
7060 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2539 3716 3777 3821 4143 4539 4578 5173 5954
Size: 1177 61 44 322 396 39 595 781
Cuts at: 5954 8399 9672 9757 10828 2539
Size: 2445 1273 85 1071 3322
Fragments arranged by size:
3322 2445 1273 1177 1071 781 595 396
322 85 61 44 39
BsmI GAATG_Cn'
Cuts at: 3710 5209 5413 6721 7185 9122 9215 3710
Size: 1499 204 1308 464 1937 93 6106
Fragments arranged by size:
6106 1937 1499 1308 464 204 93
BsmBI CGTCTCn'nnnn_
Cuts at: 6327 6327
Size: 11611
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2539 3698 3716 3777 3821 4087 4143
Size: 399 749 1159 18 61 44 266 56
Cuts at: 4143 4539 4578 5173 5452 5552 5733 5954 7133
Size: 396 39 595 279 100 181 221 1179
Cuts at: 7133 7652 8082 8399 8930 9672 9757 10828 1391
Size: 519 430 317 531 742 85 1071 2174
Fragments arranged by size:
2174 1179 1159 1071 749 742 595 531
519 430 399 396 317 279 266 221
181 100 85 61 56 44 39 18
BspEI T'CCGG_A
Cuts at: 4326 11274 4326
Size: 6948 4663
BspHI T'CATG_A
Cuts at: 4173 5277 9500 11230 4173
Size: 1104 4223 1730 4554
Fragments arranged by size:
4554 4223 1730 1104
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 6447 6739 7305 8707 8966 844
Size: 5603 292 566 1402 259 3489
Fragments arranged by size:
5603 3489 1402 566 292 259
BsrBI CCG'CTC
Cuts at: 1394 1823 9498 1394
Size: 429 7675 3507
Fragments arranged by size:
7675 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3341 4922 5907 6473 7494 10106 10280
Size: 1183 2093 1581 985 566 1021 2612 174
Cuts at: 10280 65
Size: 1396
Fragments arranged by size:
2612 2093 1581 1396 1183 1021 985 566
174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3404 10255 10442 1700
Size: 110 1594 6851 187 2869
Fragments arranged by size:
6851 2869 1594 187 110
BsrGI T'GTAC_A
Cuts at: 96 2379 96
Size: 2283 9328
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 11611
BssSI C'ACGA_G
Cuts at: 6002 9671 10683 6002
Size: 3669 1012 6930
Fragments arranged by size:
6930 3669 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5071 5204 8128 794
Size: 4277 133 2924 4277
Fragments arranged by size:
4277 4277 2924 133
BstBI TT'CG_AA
Cuts at: 7794 10415 7794
Size: 2621 8990
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 11611
BstXI CCAn_nnnn'nTGG
Cuts at: 2348 3433 7539 2348
Size: 1085 4106 6420
Fragments arranged by size:
6420 4106 1085
BstYI r'GATC_y
Cuts at: 1972 3254 3470 3790 4813 5707 5818 6381 7588
Size: 1282 216 320 1023 894 111 563 1207
Cuts at: 7588 7603 7798 8941 9308 9693 9710 11162 11248
Size: 15 195 1143 367 385 17 1452 86
Cuts at: 11248 11260 11606 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1282 1207 1143 1023 894 563
385 367 346 320 216 195 111 86
17 15 12
BstZ17I GTA'TAC
Cuts at: 3027 4837 3027
Size: 1810 9801
Bsu36I CC'TnA_GG
Cuts at: 3956 3956
Size: 11611
BtgI C'CryG_G
Cuts at: 513 1065 2484 3301 3855 4187 4525 4599 7423
Size: 552 1419 817 554 332 338 74 2824
Cuts at: 7423 513
Size: 4701
Fragments arranged by size:
4701 2824 1419 817 554 552 338 332
74
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5398 487
Size: 683 56 4172 6700
Fragments arranged by size:
6700 4172 683 56
BtsI GCAGTG_nn'
Cuts at: 2765 3228 4830 5072 5733 6022 6424 8481 9127
Size: 463 1602 242 661 289 402 2057 646
Cuts at: 9127 9921 9941 2765
Size: 794 20 4435
Fragments arranged by size:
4435 2057 1602 794 661 646 463 402
289 242 20
ClaI AT'CG_AT
Cuts at: 1554 3573 6455 1554
Size: 2019 2882 6710
Fragments arranged by size:
6710 2882 2019
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 8657 8692 529
Size: 35 8093 35 3448
Fragments arranged by size:
8093 3448 35 35
DraI TTT'AAA
Cuts at: 2950 3975 7479 7881 8915 2950
Size: 1025 3504 402 1034 5646
Fragments arranged by size:
5646 3504 1034 1025 402
DraIII CAC_nnn'GTG
Cuts at: 4870 4872 4969 5140 4870
Size: 2 97 171 11341
Fragments arranged by size:
11341 171 97 2
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 10618 809
Size: 507 9302 1802
Fragments arranged by size:
9302 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2724 3064 3519
Size: 54 1425 50 275 18 894 340 455
Cuts at: 3519 3897 9058 9947 11576 8
Size: 378 5161 889 1629 43
Fragments arranged by size:
5161 1629 1425 894 889 455 378 340
275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 9058 1537
Size: 275 7246 4090
Fragments arranged by size:
7246 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3098 3223 5003 5440 6741 7396 8008 8259
Size: 1745 125 1780 437 1301 655 612 251
Cuts at: 8259 8298 8349 8571 9541 1353
Size: 39 51 222 970 3423
Fragments arranged by size:
3423 1780 1745 1301 970 655 612 437
251 222 125 51 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5602 10198 10572 10718 387
Size: 1064 138 4013 4596 374 146 1280
Fragments arranged by size:
4596 4013 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3714 3819 4141 4537 8397 3714
Size: 105 322 396 3860 6928
Fragments arranged by size:
6928 3860 396 322 105
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2034 4046 4154 4367 4372 4634 4640
Size: 393 402 2012 108 213 5 262 6
Cuts at: 4640 4922 4984 5723 5789 5969 6833 7515 7886
Size: 282 62 739 66 180 864 682 371
Cuts at: 7886 8424 8869 9674 10271 11058 11326 1239
Size: 538 445 805 597 787 268 1524
Fragments arranged by size:
2012 1524 864 805 787 739 682 597
538 445 402 393 371 282 268 262
213 180 108 66 62 6 5
EcoNI CCTnn'n_nnAGG
Cuts at: 4589 5096 4589
Size: 507 11104
EcoO109I rG'GnC_Cy
Cuts at: 3509 5472 5698 6286 8065 8635 3509
Size: 1963 226 588 1779 570 6485
Fragments arranged by size:
6485 1963 1779 588 570 226
EcoRI G'AATT_C
Cuts at: 2421 2457 4282 8920 9298 2421
Size: 36 1825 4638 378 4734
Fragments arranged by size:
4734 4638 1825 378 36
EcoRV GAT'ATC
Cuts at: 5898 6655 7892 5898
Size: 757 1237 9617
Fragments arranged by size:
9617 1237 757
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4742 6403 6629 4742
Size: 1661 226 9724
Fragments arranged by size:
9724 1661 226
FspI TGC'GCA
Cuts at: 10117 10117
Size: 11611
HaeII r_GCGC'y
Cuts at: 1678 4164 10758 1678
Size: 2486 6594 2531
Fragments arranged by size:
6594 2531 2486
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2326 2358 3097 3129 3169
Size: 32 135 32 754 32 739 32 40
Cuts at: 3169 3201 3246 3278 4148 4180 4280 4312 4321
Size: 32 45 32 870 32 100 32 9
Cuts at: 4321 4353 5485 5517 5939 5971 6770 6802 7130
Size: 32 1132 32 422 32 799 32 328
Cuts at: 7130 7162 7447 7479 10252 10284 10326 10358 1373
Size: 32 285 32 2773 32 42 32 2626
Fragments arranged by size:
2773 2626 1132 870 799 754 739 422
328 285 135 100 45 42 40 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 9
HincII GTy'rAC
Cuts at: 669 1657 1938 2584 2824 3153 3664 4306 5826
Size: 988 281 646 240 329 511 642 1520
Cuts at: 5826 7047 7687 8790 8955 9201 669
Size: 1221 640 1103 165 246 3079
Fragments arranged by size:
3079 1520 1221 1103 988 646 642 640
511 329 281 246 240 165
HindIII A'AGCT_T
Cuts at: 748 4178 6845 7875 748
Size: 3430 2667 1030 4484
Fragments arranged by size:
4484 3430 2667 1030
HpaI GTT'AAC
Cuts at: 9201 9201
Size: 11611
KasI G'GCGC_C
Cuts at: 4160 4160
Size: 11611
KpnI G_GTAC'C
Cuts at: 8936 8936
Size: 11611
MfeI C'AATT_G
Cuts at: 3042 5781 9210 3042
Size: 2739 3429 5443
Fragments arranged by size:
5443 3429 2739
MluI A'CGCG_T
Cuts at: 11487 11487
Size: 11611
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3232 4425 7419 10725 10909 1305
Size: 1927 1193 2994 3306 184 2007
Fragments arranged by size:
3306 2994 2007 1927 1193 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2726 3066 3521 3899 10
Size: 54 1425 343 894 340 455 378 7722
Fragments arranged by size:
7722 1425 894 455 378 343 340 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2042 2346 3620 6077 7479 9569 9928
Size: 648 876 304 1274 2457 1402 2090 359
Cuts at: 9928 10087 518
Size: 159 2042
Fragments arranged by size:
2457 2090 2042 1402 1274 876 648 359
304 159
MspA1I CmG'CkG
Cuts at: 1606 2755 3551 4189 4641 4748 5004 5468 7354
Size: 1149 796 638 452 107 256 464 1886
Cuts at: 7354 7713 7844 7906 8292 8632 9027 9704 10852
Size: 359 131 62 386 340 395 677 1148
Cuts at: 10852 11097 1606
Size: 245 2120
Fragments arranged by size:
2120 1886 1149 1148 796 677 638 464
452 395 386 359 340 256 245 131
107 62
NarI GG'CG_CC
Cuts at: 4161 4161
Size: 11611
NcoI C'CATG_G
Cuts at: 513 1065 2484 3301 3855 4525 7423 513
Size: 552 1419 817 554 670 2898 4701
Fragments arranged by size:
4701 2898 1419 817 670 554 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 11611
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 11611
NotI GC'GGCC_GC
Cuts at: 9058 9058
Size: 11611
NsiI A_TGCA'T
Cuts at: 7060 7060
Size: 11611
NspI r_CATG'y
Cuts at: 1132 1229 3896 4900 7702 8969 10514 1132
Size: 97 2667 1004 2802 1267 1545 2229
Fragments arranged by size:
2802 2667 2229 1545 1267 1004 97
PasI CC'CwG_GG
Cuts at: 1348 5239 7133 8069 1348
Size: 3891 1894 936 4890
Fragments arranged by size:
4890 3891 1894 936
PciI A'CATG_T
Cuts at: 10510 10510
Size: 11611
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2663 4121 5562 1108
Size: 1555 1458 1441 7157
Fragments arranged by size:
7157 1555 1458 1441
PfoI T'CCnGG_A
Cuts at: 4388 7036 4388
Size: 2648 8963
PmeI GTTT'AAAC
Cuts at: 8915 8915
Size: 11611
PmlI CAC'GTG
Cuts at: 4082 4082
Size: 11611
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 9680 9712 11193 11225 9680
Size: 32 1481 32 10066
Fragments arranged by size:
10066 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 5472 5698 6286 8065 8635 5472
Size: 226 588 1779 570 8448
Fragments arranged by size:
8448 1779 588 570 226
PshAI GACnn'nnGTC
Cuts at: 1613 6097 1613
Size: 4484 7127
PsiI TTA'TAA
Cuts at: 4430 6705 9181 4430
Size: 2275 2476 6860
Fragments arranged by size:
6860 2476 2275
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 6174 6206 6174
Size: 32 11579
PstI C_TGCA'G
Cuts at: 830 2083 2760 3582 4593 6189 7612 8133 8963
Size: 1253 677 822 1011 1596 1423 521 830
Cuts at: 8963 830
Size: 3478
Fragments arranged by size:
3478 1596 1423 1253 1011 830 822 677
521
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 11611
PvuII CAG'CTG
Cuts at: 2755 3551 4748 5468 7354 7713 7906 8292 2755
Size: 796 1197 720 1886 359 193 386 6074
Fragments arranged by size:
6074 1886 1197 796 720 386 359 193
SacI G_AGCT'C
Cuts at: 3716 3821 4143 4539 8399 3716
Size: 105 322 396 3860 6928
Fragments arranged by size:
6928 3860 396 322 105
SacII CC_GC'GG
Cuts at: 4190 4190
Size: 11611
SalI G'TCGA_C
Cuts at: 1936 3662 7685 8953 1936
Size: 1726 4023 1268 4594
Fragments arranged by size:
4594 4023 1726 1268
SanDI GG'GwC_CC
Cuts at: 5472 8065 5472
Size: 2593 9018
SapI GCTCTTCn'nnn_
Cuts at: 5003 5440 5003
Size: 437 11174
SbfI CC_TGCA'GG
Cuts at: 8963 8963
Size: 11611
ScaI AGT'ACT
Cuts at: 3460 9859 11571 3460
Size: 6399 1712 3500
Fragments arranged by size:
6399 3500 1712
SfcI C'TryA_G
Cuts at: 826 1045 2079 2756 3578 4589 6185 6277 7608
Size: 219 1034 677 822 1011 1596 92 1331
Cuts at: 7608 8129 8530 8959 10094 10775 10966 826
Size: 521 401 429 1135 681 191 1471
Fragments arranged by size:
1596 1471 1331 1135 1034 1011 822 681
677 521 429 401 219 191 92
SfoI GGC'GCC
Cuts at: 4162 4162
Size: 11611
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11611
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 11611
SmaI CCC'GGG
Cuts at: 1872 8938 1872
Size: 7066 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5373 8392 8702 8730 8771 9715
Size: 197 926 3430 3019 310 28 41 944
Cuts at: 9715 10616 10878 820
Size: 901 262 1553
Fragments arranged by size:
3430 3019 1553 944 926 901 310 262
197 41 28
SnaBI TAC'GTA
Cuts at: 493 9402 493
Size: 8909 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 11611
SphI G_CATG'C
Cuts at: 1132 7702 8969 1132
Size: 6570 1267 3774
Fragments arranged by size:
6570 3774 1267
SspI AAT'ATT
Cuts at: 5 52 2116 8201 8525 9535 5
Size: 47 2064 6085 324 1010 2081
Fragments arranged by size:
6085 2081 2064 1010 324 47
StuI AGG'CCT
Cuts at: 1517 4517 1517
Size: 3000 8611
StyI C'CwwG_G
Cuts at: 513 1065 2484 3301 3855 4525 4544 6013 7423
Size: 552 1419 817 554 670 19 1469 1410
Cuts at: 7423 8112 8853 9050 513
Size: 689 741 197 3074
Fragments arranged by size:
3074 1469 1419 1410 817 741 689 670
554 552 197 19
SwaI ATTT'AAAT
Cuts at: 7479 7479
Size: 11611
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3814 8861 9638 9655 9808 9993 10438
Size: 1255 2234 5047 777 17 153 185 445
Cuts at: 10438 325
Size: 1498
Fragments arranged by size:
5047 2234 1498 1255 777 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2379 3396 3458
Size: 274 80 33 51 1443 402 1017 62
Cuts at: 3458 4062 4732 9857 11569 96
Size: 604 670 5125 1712 138
Fragments arranged by size:
5125 1712 1443 1017 670 604 402 274
138 80 62 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2390 2422 8636 8668 11571 11603 2390
Size: 32 6214 32 2903 32 2398
Fragments arranged by size:
6214 2903 2398 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 11611
XbaI T'CTAG_A
Cuts at: 8947 8947
Size: 11611
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3619 5559 6124 7660 8252 8893 3619
Size: 1940 565 1536 592 641 6337
Fragments arranged by size:
6337 1940 1536 641 592 565
XhoI C'TCGA_G
Cuts at: 1943 8730 1943
Size: 6787 4824
XmaI C'CCGG_G
Cuts at: 1870 8936 1870
Size: 7066 4545
XmnI GAAnn'nnTTC
Cuts at: 2425 4517 7171 7185 9740 2425
Size: 2092 2654 14 2555 4296
Fragments arranged by size:
4296 2654 2555 2092 14
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5496 7690 9419 276
Size: 53 83 186 936 3962 2194 1729 2468
Fragments arranged by size:
3962 2468 2194 1729 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI
PstI PvuI PvuII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BsiWI FseI
FspAI NaeI NgoMIV NruI PacI PspOMI PspXI RsrII
SexAI SfiI SrfI