Restriction map for
pFN21AA1507
(Circular) (Six-base) MAPSORT of: pFN21AA1507.seq Check: 288 from: 1 to: 7471
pFN21AA1507 7471 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2787 4265 5281 278
Size: 53 83 186 936 1251 1478 1016 2468
Fragments arranged by size:
2468 1478 1251 1016 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3363 4431 4814 1937
Size: 1426 1068 383 4594
Fragments arranged by size:
4594 1426 1068 383
Acc65I G'GTAC_C
Cuts at: 4792 4792
Size: 7471
AclI AA'CG_TT
Cuts at: 1563 5598 5971 1563
Size: 4035 373 3063
Fragments arranged by size:
4035 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4034 4177 5534 6918 7186 1632
Size: 2402 143 1357 1384 268 1917
Fragments arranged by size:
2402 1917 1384 1357 268 143
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7274
AflIII A'CryG_T
Cuts at: 1203 3217 6370 7347 1203
Size: 2014 3153 977 1327
Fragments arranged by size:
3153 2014 1327 977
AgeI A'CCGG_T
Cuts at: 6302 6302
Size: 7471
AhdI GACnn_n'nnGTC
Cuts at: 3001 6200 3001
Size: 3199 4272
AleI CACnn'nnGTG
Cuts at: 1166 2378 3779 1166
Size: 1212 1401 4858
Fragments arranged by size:
4858 1401 1212
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7471
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3491 3523 3491
Size: 32 7439
AlwNI CAG_nnn'CTG
Cuts at: 2969 4184 4204 4492 5214 6786 2969
Size: 1215 20 288 722 1572 3654
Fragments arranged by size:
3654 1572 1215 722 288 20
ApaI G_GGCC'C
Cuts at: 2299 2299
Size: 7471
ApaLI G'TGCA_C
Cuts at: 5528 6684 5528
Size: 1156 6315
ApoI r'AATT_y
Cuts at: 1493 2326 2916 4780 5006 5158 7267 1493
Size: 833 590 1864 226 152 2109 1697
Fragments arranged by size:
2109 1864 1697 833 590 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6025 160
Size: 872 4993 1606
Fragments arranged by size:
4993 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7471
AvaI C'yCGr_G
Cuts at: 1870 1943 2015 2830 2889 3613 3634 4152 4796
Size: 73 72 815 59 724 21 518 644
Cuts at: 4796 1870
Size: 4545
Fragments arranged by size:
4545 815 724 644 518 73 72 59
21
AvrII C'CTAG_G
Cuts at: 3808 4466 3808
Size: 658 6813
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2502 2535 2502
Size: 33 7438
BamHI G'GATC_C
Cuts at: 3267 4495 4801 5168 3267
Size: 1228 306 367 5570
Fragments arranged by size:
5570 1228 367 306
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2755 2879 3298 3770 4075
Size: 325 217 627 968 124 419 472 305
Cuts at: 4075 4792 6247 618
Size: 717 1455 1842
Fragments arranged by size:
1842 1455 968 717 627 472 419 325
305 217 124
BanII G_rGCy'C
Cuts at: 1391 2299 3807 4011 4790 1391
Size: 908 1508 204 779 4072
Fragments arranged by size:
4072 1508 908 779 204
BbeI G_GCGC'C
Cuts at: 2759 2759
Size: 7471
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2739 928
Size: 971 840 5660
Fragments arranged by size:
5660 971 840
BbvCI CC'TCA_GC
Cuts at: 2825 3458 3560 4185 2825
Size: 633 102 625 6111
Fragments arranged by size:
6111 633 625 102
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5662 5696 1903
Size: 34 3725 34 3678
Fragments arranged by size:
3725 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5363 6573 5363
Size: 1210 6261
BclI T'GATC_A
Cuts at: 1548 4335 1548
Size: 2787 4684
BfrBI ATG'CAT
Cuts at: 4382 4382
Size: 7471
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3796 6082 136
Size: 107 122 71 631 605 2124 2286 1525
Fragments arranged by size:
2286 2124 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7466 7466
Size: 7471
BlpI GC'TnA_GC
Cuts at: 3555 3980 4888 3555
Size: 425 908 6138
Fragments arranged by size:
6138 908 425
Bme1580I G_kGCm'C
Cuts at: 1790 2299 2545 2882 3301 3773 4410 4561 5532
Size: 509 246 337 419 472 637 151 971
Cuts at: 5532 6688 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 971 637 509 472 419 337
246 151
BmgBI CAC'GTC
Cuts at: 1318 2389 3781 4027 1318
Size: 1071 1392 246 4762
Fragments arranged by size:
4762 1392 1071 246
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2302 2458 3359
Size: 416 53 83 399 24 885 156 901
Cuts at: 3359 5202 6160 442
Size: 1843 958 1753
Fragments arranged by size:
1843 1753 958 901 885 416 399 156
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7471
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3649 3747 3649
Size: 98 7373
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2237 3499 3503 3977 4055 4307 6131 1239
Size: 998 1262 4 474 78 252 1824 2579
Fragments arranged by size:
2579 1824 1262 998 474 252 78 4
Bpu10I CC'TnA_GC
Cuts at: 2157 2825 3458 3560 3801 4185 4511 2157
Size: 668 633 102 241 384 326 5117
Fragments arranged by size:
5117 668 633 384 326 241 102
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3676 5596 6461 6759 3676
Size: 1920 865 298 4388
Fragments arranged by size:
4388 1920 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3009 3748 6134 882
Size: 617 1510 739 2386 2219
Fragments arranged by size:
2386 2219 1510 739 617
BsaAI yAC'GTr
Cuts at: 493 1204 5262 493
Size: 711 4058 2702
Fragments arranged by size:
4058 2702 711
BsaBI GATnn'nnATC
Cuts at: 7465 7465
Size: 7471
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 2400 2756 2784
Size: 53 83 186 936 476 391 356 28
Cuts at: 2784 4262 5278 5660 6295 275
Size: 1478 1016 382 635 1451
Fragments arranged by size:
1478 1451 1016 936 635 476 391 382
356 186 83 53 28
BsaWI w'CCGG_w
Cuts at: 3399 3733 5904 6302 6576 6723 7134 3399
Size: 334 2171 398 274 147 411 3736
Fragments arranged by size:
3736 2171 411 398 334 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2235 2265 3491 3521 4028
Size: 30 165 30 832 30 1226 30 507
Cuts at: 4028 4058 1178
Size: 30 4591
Fragments arranged by size:
4591 1226 832 507 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2450 2545 3031 3392 3601 4091 1186
Size: 1264 95 486 361 209 490 4566
Fragments arranged by size:
4566 1264 490 486 361 209 95
BseYI C'CCAG_C
Cuts at: 2455 2700 2805 2933 3711 3996 4268 6674 7170
Size: 245 105 128 778 285 272 2406 496
Cuts at: 7170 2455
Size: 2756
Fragments arranged by size:
2756 2406 778 496 285 272 245 128
105
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3641 4007 1163
Size: 573 1905 366 4627
Fragments arranged by size:
4627 1905 573 366
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4921 5682 6710 1540
Size: 275 186 2920 761 1028 2301
Fragments arranged by size:
2920 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2394 3977 4633 5532 5617 6688 2394
Size: 1583 656 899 85 1071 3177
Fragments arranged by size:
3177 1583 1071 899 656 85
BsmI GAATG_Cn'
Cuts at: 2020 3591 4982 5075 2020
Size: 1571 1391 93 4416
Fragments arranged by size:
4416 1571 1391 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2059 2219 3731 4033 4756 2059
Size: 160 1512 302 723 4774
Fragments arranged by size:
4774 1512 723 302 160
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2299 2394 2545 2882 3301 3773 3807
Size: 399 509 95 151 337 419 472 34
Cuts at: 3807 3977 4011 4410 4561 4633 4790 5532 5617
Size: 170 34 399 151 72 157 742 85
Cuts at: 5617 6688 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 509 472 419 399 399
337 170 157 151 151 95 85 72
34 34
BspEI T'CCGG_A
Cuts at: 3733 7134 3733
Size: 3401 4070
BspHI T'CATG_A
Cuts at: 4444 5360 7090 4444
Size: 916 1730 4825
Fragments arranged by size:
4825 1730 916
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4826 844
Size: 3982 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3793 5358 1394
Size: 429 1970 1565 3507
Fragments arranged by size:
3507 1970 1565 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2082 4330 5966 6140 65
Size: 1183 834 2248 1636 174 1396
Fragments arranged by size:
2248 1636 1396 1183 834 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3886 6115 6302 1700
Size: 110 2076 2229 187 2869
Fragments arranged by size:
2869 2229 2076 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7471
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7471
BssSI C'ACGA_G
Cuts at: 2621 5531 6543 2621
Size: 2910 1012 3549
Fragments arranged by size:
3549 2910 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7471
BstBI TT'CG_AA
Cuts at: 3583 6275 3583
Size: 2692 4779
BstEII G'GTnAC_C
Cuts at: 1184 4505 1184
Size: 3321 4150
BstXI CCAn_nnnn'nTGG
Cuts at: 2410 3781 4620 2410
Size: 1371 839 5261
Fragments arranged by size:
5261 1371 839
BstYI r'GATC_y
Cuts at: 1972 3267 4495 4801 5168 5553 5570 7022 7108
Size: 1295 1228 306 367 385 17 1452 86
Cuts at: 7108 7120 7466 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1295 1228 385 367 346 306
86 17 12
BstZ17I GTA'TAC
Cuts at: 4432 4432
Size: 7471
Bsu36I CC'TnA_GG
Cuts at: 2301 2333 3677 2301
Size: 32 1344 6095
Fragments arranged by size:
6095 1344 32
BtgI C'CryG_G
Cuts at: 513 1065 2004 3148 3528 4638 513
Size: 552 939 1144 380 1110 3346
Fragments arranged by size:
3346 1144 1110 939 552 380
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4560 487
Size: 683 56 3334 3398
Fragments arranged by size:
3398 3334 683 56
BtsI GCAGTG_nn'
Cuts at: 2849 4653 4987 5781 5801 2849
Size: 1804 334 794 20 4519
Fragments arranged by size:
4519 1804 794 334 20
ClaI AT'CG_AT
Cuts at: 1554 3966 1554
Size: 2412 5059
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2607 2642 4035 4070 529
Size: 35 2043 35 1393 35 3930
Fragments arranged by size:
3930 2043 1393 35 35 35
DraI TTT'AAA
Cuts at: 4775 4775
Size: 7471
DraIII CAC_nnn'GTG
Cuts at: 4505 4505
Size: 7471
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2695 2741 6478 809
Size: 507 1379 46 3737 1802
Fragments arranged by size:
3737 1802 1379 507 46
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2079 3598 4299
Size: 54 1425 50 275 18 249 1519 701
Cuts at: 4299 4641 4918 5807 7436 8
Size: 342 277 889 1629 43
Fragments arranged by size:
1629 1519 1425 889 701 342 277 275
249 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4918 1537
Size: 275 3106 4090
Fragments arranged by size:
4090 3106 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2427 3257 4133 4679 5401 1353
Size: 1074 830 876 546 722 3423
Fragments arranged by size:
3423 1074 876 830 722 546
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6058 6432 6578 387
Size: 1064 138 4469 374 146 1280
Fragments arranged by size:
4469 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2237 3499 3503 3977 4034 4055 4177
Size: 393 605 1262 4 474 57 21 122
Cuts at: 4177 4307 5534 6131 6918 7186 1239
Size: 130 1227 597 787 268 1524
Fragments arranged by size:
1524 1262 1227 787 605 597 474 393
268 130 122 57 21 4
EcoNI CCTnn'n_nnAGG
Cuts at: 3227 3445 3227
Size: 218 7253
EcoO109I rG'GnC_Cy
Cuts at: 2296 2347 2452 2866 3472 3694 4158 4201 4254
Size: 51 105 414 606 222 464 43 53
Cuts at: 4254 2296
Size: 5513
Fragments arranged by size:
5513 606 464 414 222 105 53 51
43
EcoRI G'AATT_C
Cuts at: 4780 5158 4780
Size: 378 7093
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3688 3688
Size: 7471
FspI TGC'GCA
Cuts at: 2777 5977 2777
Size: 3200 4271
FspAI rTGC'GCAy
Cuts at: 2777 2777
Size: 7471
HaeII r_GCGC'y
Cuts at: 1678 2759 6618 1678
Size: 1081 3859 2531
Fragments arranged by size:
3859 2531 1081
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2982 2987 3014 3019 3596
Size: 32 135 32 1410 5 27 5 577
Cuts at: 3596 3628 4028 4060 4132 4164 4680 4712 6112
Size: 32 400 32 72 32 516 32 1400
Cuts at: 6112 6144 6186 6218 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1410 1400 577 516 400 135 72
42 32 32 32 32 32 32 32
32 27 5 5
HincII GTy'rAC
Cuts at: 669 1657 1938 3114 3364 4065 4815 5061 669
Size: 988 281 1176 250 701 750 246 3079
Fragments arranged by size:
3079 1176 988 750 701 281 250 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7471
HpaI GTT'AAC
Cuts at: 5061 5061
Size: 7471
KasI G'GCGC_C
Cuts at: 2755 2755
Size: 7471
KpnI G_GTAC'C
Cuts at: 4796 4796
Size: 7471
MfeI C'AATT_G
Cuts at: 5070 5070
Size: 7471
MluI A'CGCG_T
Cuts at: 7347 7347
Size: 7471
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2198 4054 6585 6769 1305
Size: 893 1856 2531 184 2007
Fragments arranged by size:
2531 2007 1856 893 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2081 4301 10
Size: 54 1425 343 249 2220 3180
Fragments arranged by size:
3180 2220 1425 343 249 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2378 3216 3779 4178 4223 5429 5788
Size: 648 1212 838 563 399 45 1206 359
Cuts at: 5788 5947 518
Size: 159 2042
Fragments arranged by size:
2042 1212 1206 838 648 563 399 359
159 45
MspA1I CmG'CkG
Cuts at: 1606 2075 2459 2937 3047 3996 4268 4887 5564
Size: 469 384 478 110 949 272 619 677
Cuts at: 5564 6712 6957 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 949 677 619 478 469 384
272 245 110
NaeI GCC'GGC
Cuts at: 3888 3888
Size: 7471
NarI GG'CG_CC
Cuts at: 2756 2756
Size: 7471
NcoI C'CATG_G
Cuts at: 513 1065 2004 4638 513
Size: 552 939 2634 3346
Fragments arranged by size:
3346 2634 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7471
NgoMIV G'CCGG_C
Cuts at: 3886 3886
Size: 7471
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7471
NotI GC'GGCC_GC
Cuts at: 4918 4918
Size: 7471
NsiI A_TGCA'T
Cuts at: 4384 4384
Size: 7471
NspI r_CATG'y
Cuts at: 1132 1229 3221 3988 4382 4414 4829 6374 1132
Size: 97 1992 767 394 32 415 1545 2229
Fragments arranged by size:
2229 1992 1545 767 415 394 97 32
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7471
PciI A'CATG_T
Cuts at: 3217 6370 3217
Size: 3153 4318
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4204 4222 1108
Size: 3096 18 4357
Fragments arranged by size:
4357 3096 18
PfoI T'CCnGG_A
Cuts at: 2253 2727 4491 2253
Size: 474 1764 5233
Fragments arranged by size:
5233 1764 474
PmeI GTTT'AAAC
Cuts at: 4775 4775
Size: 7471
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3491 3523 5540 5572 7053 7085 3491
Size: 32 2017 32 1481 32 3877
Fragments arranged by size:
3877 2017 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2452 2866 3472 4201 2452
Size: 414 606 729 5722
Fragments arranged by size:
5722 729 606 414
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7471
PsiI TTA'TAA
Cuts at: 5041 5041
Size: 7471
PspOMI G'GGCC_C
Cuts at: 2295 2295
Size: 7471
PspXI vC'TCGA_Gb
Cuts at: 4152 4152
Size: 7471
PstI C_TGCA'G
Cuts at: 830 2268 3018 3308 3468 4525 4823 830
Size: 1438 750 290 160 1057 298 3478
Fragments arranged by size:
3478 1438 1057 750 298 290 160
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7471
PvuII CAG'CTG
Cuts at: 2075 2459 4268 2075
Size: 384 1809 5278
Fragments arranged by size:
5278 1809 384
SalI G'TCGA_C
Cuts at: 1936 3362 4813 1936
Size: 1426 1451 4594
Fragments arranged by size:
4594 1451 1426
SanDI GG'GwC_CC
Cuts at: 2452 3472 2452
Size: 1020 6451
SapI GCTCTTCn'nnn_
Cuts at: 4133 4133
Size: 7471
SbfI CC_TGCA'GG
Cuts at: 3308 4823 3308
Size: 1515 5956
ScaI AGT'ACT
Cuts at: 3671 5719 7431 3671
Size: 2048 1712 3711
Fragments arranged by size:
3711 2048 1712
SexAI A'CCwGG_T
Cuts at: 2232 4501 2232
Size: 2269 5202
SfcI C'TryA_G
Cuts at: 826 1045 2264 3014 3304 3442 3464 4521 4660
Size: 219 1219 750 290 138 22 1057 139
Cuts at: 4660 4819 5954 6635 6826 826
Size: 159 1135 681 191 1471
Fragments arranged by size:
1471 1219 1135 1057 750 681 290 219
191 159 139 138 22
SfoI GGC'GCC
Cuts at: 2757 2757
Size: 7471
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7471
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7471
SmaI CCC'GGG
Cuts at: 1872 2017 2832 2891 4798 1872
Size: 145 815 59 1907 4545
Fragments arranged by size:
4545 1907 815 145 59
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3655 4152 5575 6476 6738 820
Size: 197 926 1712 497 1423 901 262 1553
Fragments arranged by size:
1712 1553 1423 926 901 497 262 197
SnaBI TAC'GTA
Cuts at: 493 5262 493
Size: 4769 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7471
SphI G_CATG'C
Cuts at: 1132 3988 4382 4829 1132
Size: 2856 394 447 3774
Fragments arranged by size:
3774 2856 447 394
SrfI GCCC'GGGC
Cuts at: 2017 2832 2017
Size: 815 6656
SspI AAT'ATT
Cuts at: 5 52 5395 5
Size: 47 5343 2081
Fragments arranged by size:
5343 2081 47
StuI AGG'CCT
Cuts at: 1517 2407 4569 4617 1517
Size: 890 2162 48 4371
Fragments arranged by size:
4371 2162 890 48
StyI C'CwwG_G
Cuts at: 513 1065 2004 2708 3808 4296 4353 4466 4638
Size: 552 939 704 1100 488 57 113 172
Cuts at: 4638 4910 513
Size: 272 3074
Fragments arranged by size:
3074 1100 939 704 552 488 272 172
113 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5498 5515 5668 5853 6298 325
Size: 1255 3918 17 153 185 445 1498
Fragments arranged by size:
3918 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3096 3669 5717
Size: 274 80 33 51 1443 1119 573 2048
Cuts at: 5717 7429 96
Size: 1712 138
Fragments arranged by size:
2048 1712 1443 1119 573 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7431 7463 7431
Size: 32 7439
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7471
XbaI T'CTAG_A
Cuts at: 4807 4807
Size: 7471
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2089 2380 4309 2089
Size: 291 1929 5251
Fragments arranged by size:
5251 1929 291
XhoI C'TCGA_G
Cuts at: 1943 4152 1943
Size: 2209 5262
XmaI C'CCGG_G
Cuts at: 1870 2015 2830 2889 4796 1870
Size: 145 815 59 1907 4545
Fragments arranged by size:
4545 1907 815 145 59
XmnI GAAnn'nnTTC
Cuts at: 3506 5600 3506
Size: 2094 5377
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2785 4263 5279 276
Size: 53 83 186 936 1251 1478 1016 2468
Fragments arranged by size:
2468 1478 1251 1016 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI BsiWI EcoICRI EcoRV FseI NruI
PacI PmlI PsrI RsrII SacI SacII SfiI SwaI