(Circular) (Six-base) MAPSORT of: pFN21AA1489.seq Check: 4190 from: 1 to: 6574 pFN21AA1489 6574 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4384 278 Size: 53 83 186 936 2848 2468 Fragments arranged by size: 2848 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3917 1937 Size: 1980 4594 Acc65I G'GTAC_C Cuts at: 3895 3895 Size: 6574 AclI AA'CG_TT Cuts at: 1563 2359 4701 5074 1563 Size: 796 2342 373 3063 Fragments arranged by size: 3063 2342 796 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2416 2814 3008 3019 3375 3474 4637 6021 Size: 784 398 194 11 356 99 1163 1384 Cuts at: 6021 6289 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1163 784 398 356 268 194 99 11 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6377 AflIII A'CryG_T Cuts at: 1203 2156 2505 3712 5473 6450 1203 Size: 953 349 1207 1761 977 1327 Fragments arranged by size: 1761 1327 1207 977 953 349 AgeI A'CCGG_T Cuts at: 3681 5405 3681 Size: 1724 4850 AhdI GACnn_n'nnGTC Cuts at: 3685 5303 3685 Size: 1618 4956 AleI CACnn'nnGTG Cuts at: 1166 2489 1166 Size: 1323 5251 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3285 1184 Size: 2101 4473 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2465 2497 2465 Size: 32 6542 AlwNI CAG_nnn'CTG Cuts at: 3497 4317 5889 3497 Size: 820 1572 4182 Fragments arranged by size: 4182 1572 820 ApaLI G'TGCA_C Cuts at: 4631 5787 4631 Size: 1156 5418 ApoI r'AATT_y Cuts at: 1493 2126 3414 3504 3598 3883 4109 4261 6370 Size: 633 1288 90 94 285 226 152 2109 Cuts at: 6370 1493 Size: 1697 Fragments arranged by size: 2109 1697 1288 633 285 226 152 94 90 AseI AT'TA_AT Cuts at: 160 1032 5128 160 Size: 872 4096 1606 Fragments arranged by size: 4096 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6574 AvaI C'yCGr_G Cuts at: 1870 1943 3899 1870 Size: 73 1956 4545 Fragments arranged by size: 4545 1956 73 BamHI G'GATC_C Cuts at: 3904 4271 3904 Size: 367 6207 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3895 5350 618 Size: 325 217 627 2108 1455 1842 Fragments arranged by size: 2108 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3893 1391 Size: 2502 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2637 3480 928 Size: 971 738 843 4022 Fragments arranged by size: 4022 971 843 738 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4765 4799 1903 Size: 34 2828 34 3678 Fragments arranged by size: 3678 2828 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4466 5676 4466 Size: 1210 5364 BclI T'GATC_A Cuts at: 1548 1548 Size: 6574 BfrBI ATG'CAT Cuts at: 2270 2270 Size: 6574 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5185 136 Size: 107 122 71 631 605 3513 1525 Fragments arranged by size: 3513 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6569 6569 Size: 6574 BlpI GC'TnA_GC Cuts at: 3991 3991 Size: 6574 Bme1580I G_kGCm'C Cuts at: 1790 2123 4635 5791 1790 Size: 333 2512 1156 2573 Fragments arranged by size: 2573 2512 1156 333 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6574 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2629 4305 5263 Size: 416 53 83 399 24 1212 1676 958 Cuts at: 5263 442 Size: 1753 Fragments arranged by size: 1753 1676 1212 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6574 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5234 1239 Size: 3995 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1998 4699 5564 5862 1998 Size: 2701 865 298 2710 Fragments arranged by size: 2710 2701 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3693 5237 882 Size: 617 2194 1544 2219 Fragments arranged by size: 2219 2194 1544 617 BsaAI yAC'GTr Cuts at: 493 1204 4365 493 Size: 711 3161 2702 Fragments arranged by size: 3161 2702 711 BsaBI GATnn'nnATC Cuts at: 6568 6568 Size: 6574 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4381 4763 5398 275 Size: 53 83 186 936 2848 382 635 1451 Fragments arranged by size: 2848 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3681 5007 5405 5679 5826 6237 3681 Size: 1326 398 274 147 411 4018 Fragments arranged by size: 4018 1326 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2465 2495 3156 3186 3840 Size: 30 165 30 1062 30 661 30 654 Cuts at: 3840 3870 1178 Size: 30 3882 Fragments arranged by size: 3882 1062 661 654 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3177 1186 Size: 1991 4583 BseYI C'CCAG_C Cuts at: 5777 6273 5777 Size: 496 6078 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3291 1163 Size: 573 1555 4446 Fragments arranged by size: 4446 1555 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4024 4785 5813 1540 Size: 275 186 2023 761 1028 2301 Fragments arranged by size: 2301 2023 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2478 4635 4720 5791 2478 Size: 2157 85 1071 3261 Fragments arranged by size: 3261 2157 1071 85 BsmI GAATG_Cn' Cuts at: 4085 4178 4085 Size: 93 6481 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2123 2478 3893 4635 4720 5791 1391 Size: 399 333 355 1415 742 85 1071 2174 Fragments arranged by size: 2174 1415 1071 742 399 355 333 85 BspEI T'CCGG_A Cuts at: 6237 6237 Size: 6574 BspHI T'CATG_A Cuts at: 3286 4463 6193 3286 Size: 1177 1730 3667 Fragments arranged by size: 3667 1730 1177 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3929 844 Size: 3085 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4461 1394 Size: 429 2638 3507 Fragments arranged by size: 3507 2638 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2295 3269 5069 5243 65 Size: 1183 1047 974 1800 174 1396 Fragments arranged by size: 1800 1396 1183 1047 974 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3681 5218 5405 1700 Size: 110 1871 1537 187 2869 Fragments arranged by size: 2869 1871 1537 187 110 BsrGI T'GTAC_A Cuts at: 96 2218 96 Size: 2122 4452 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6574 BssSI C'ACGA_G Cuts at: 4634 5646 4634 Size: 1012 5562 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6574 BstBI TT'CG_AA Cuts at: 5378 5378 Size: 6574 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6574 BstYI r'GATC_y Cuts at: 1972 3904 4271 4656 4673 6125 6211 6223 6569 Size: 1932 367 385 17 1452 86 12 346 Cuts at: 6569 1972 Size: 1977 Fragments arranged by size: 1977 1932 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2225 2225 Size: 6574 BtgI C'CryG_G Cuts at: 513 1065 3788 513 Size: 552 2723 3299 Fragments arranged by size: 3299 2723 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5835 Fragments arranged by size: 5835 683 56 BtsI GCAGTG_nn' Cuts at: 2484 4090 4884 4904 2484 Size: 1606 794 20 4154 Fragments arranged by size: 4154 1606 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6574 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6539 DraI TTT'AAA Cuts at: 2562 3878 2562 Size: 1316 5258 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3379 5581 809 Size: 507 2063 2202 1802 Fragments arranged by size: 2202 2063 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3360 4021 4910 Size: 54 1425 50 275 18 1530 661 889 Cuts at: 4910 6539 8 Size: 1629 43 Fragments arranged by size: 1629 1530 1425 889 661 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4021 1537 Size: 275 2209 4090 Fragments arranged by size: 4090 2209 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2435 2860 3774 3825 4504 1353 Size: 1082 425 914 51 679 3423 Fragments arranged by size: 3423 1082 914 679 425 51 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5161 5535 5681 387 Size: 1064 138 3572 374 146 1280 Fragments arranged by size: 3572 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2416 2814 3008 3019 3375 3474 4637 Size: 393 784 398 194 11 356 99 1163 Cuts at: 4637 5234 6021 6289 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1163 787 784 597 398 393 356 268 194 99 11 EcoO109I rG'GnC_Cy Cuts at: 3381 3381 Size: 6574 EcoRI G'AATT_C Cuts at: 2126 3883 4261 2126 Size: 1757 378 4439 Fragments arranged by size: 4439 1757 378 EcoRV GAT'ATC Cuts at: 2956 2956 Size: 6574 FspI TGC'GCA Cuts at: 5080 5080 Size: 6574 HaeII r_GCGC'y Cuts at: 1678 5721 1678 Size: 4043 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3671 3703 5215 5247 5289 Size: 32 135 32 2099 32 1512 32 42 Cuts at: 5289 5321 1373 Size: 32 2626 Fragments arranged by size: 2626 2099 1512 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3918 4164 669 Size: 988 281 1980 246 3079 Fragments arranged by size: 3079 1980 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6574 HpaI GTT'AAC Cuts at: 4164 4164 Size: 6574 KpnI G_GTAC'C Cuts at: 3899 3899 Size: 6574 MfeI C'AATT_G Cuts at: 3156 4173 3156 Size: 1017 5557 MluI A'CGCG_T Cuts at: 2505 6450 2505 Size: 3945 2629 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2037 5688 5872 1305 Size: 732 3651 184 2007 Fragments arranged by size: 3651 2007 732 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3362 10 Size: 54 1425 343 1530 3222 Fragments arranged by size: 3222 1530 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2183 2489 3299 3633 4532 4891 5050 Size: 648 1017 306 810 334 899 359 159 Cuts at: 5050 518 Size: 2042 Fragments arranged by size: 2042 1017 899 810 648 359 334 306 159 MspA1I CmG'CkG Cuts at: 1606 2518 2912 3395 3990 4667 5815 6060 1606 Size: 912 394 483 595 677 1148 245 2120 Fragments arranged by size: 2120 1148 912 677 595 483 394 245 NcoI C'CATG_G Cuts at: 513 1065 3788 513 Size: 552 2723 3299 Fragments arranged by size: 3299 2723 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6574 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6574 NotI GC'GGCC_GC Cuts at: 4021 4021 Size: 6574 NsiI A_TGCA'T Cuts at: 2272 2272 Size: 6574 NspI r_CATG'y Cuts at: 1132 1229 2160 3932 5477 1132 Size: 97 931 1772 1545 2229 Fragments arranged by size: 2229 1772 1545 931 97 PacI TTA_AT'TAA Cuts at: 2379 2379 Size: 6574 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6574 PciI A'CATG_T Cuts at: 2156 5473 2156 Size: 3317 3257 PflMI CCAn_nnn'nTGG Cuts at: 1108 2786 1108 Size: 1678 4896 PmeI GTTT'AAAC Cuts at: 3878 3878 Size: 6574 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2465 2497 4643 4675 6156 6188 2465 Size: 32 2146 32 1481 32 2851 Fragments arranged by size: 2851 2146 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3381 3381 Size: 6574 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6574 PsiI TTA'TAA Cuts at: 4144 4144 Size: 6574 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3305 3337 3305 Size: 32 6542 PstI C_TGCA'G Cuts at: 830 3400 3926 830 Size: 2570 526 3478 Fragments arranged by size: 3478 2570 526 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6574 PvuII CAG'CTG Cuts at: 2518 2912 3395 2518 Size: 394 483 5697 Fragments arranged by size: 5697 483 394 SalI G'TCGA_C Cuts at: 1936 3916 1936 Size: 1980 4594 SbfI CC_TGCA'GG Cuts at: 3926 3926 Size: 6574 ScaI AGT'ACT Cuts at: 4822 6534 4822 Size: 1712 4862 SexAI A'CCwGG_T Cuts at: 3080 3080 Size: 6574 SfcI C'TryA_G Cuts at: 826 1045 2304 2336 3396 3477 3870 3922 5057 Size: 219 1259 32 1060 81 393 52 1135 Cuts at: 5057 5738 5929 826 Size: 681 191 1471 Fragments arranged by size: 1471 1259 1135 1060 681 393 219 191 81 52 32 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6574 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6574 SmaI CCC'GGG Cuts at: 1872 3901 1872 Size: 2029 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2013 4678 5579 5841 820 Size: 197 926 70 2665 901 262 1553 Fragments arranged by size: 2665 1553 926 901 262 197 70 SnaBI TAC'GTA Cuts at: 493 4365 493 Size: 3872 2702 SpeI A'CTAG_T Cuts at: 152 3374 152 Size: 3222 3352 SphI G_CATG'C Cuts at: 1132 3932 1132 Size: 2800 3774 SspI AAT'ATT Cuts at: 5 52 2372 4498 5 Size: 47 2320 2126 2081 Fragments arranged by size: 2320 2126 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6574 StyI C'CwwG_G Cuts at: 513 1065 3335 3788 4013 513 Size: 552 2270 453 225 3074 Fragments arranged by size: 3074 2270 552 453 225 SwaI ATTT'AAAT Cuts at: 2562 2562 Size: 6574 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2734 4601 4618 4771 4956 5401 325 Size: 1255 1154 1867 17 153 185 445 1498 Fragments arranged by size: 1867 1498 1255 1154 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2218 3321 3715 Size: 274 80 33 51 1443 241 1103 394 Cuts at: 3715 4820 6532 96 Size: 1105 1712 138 Fragments arranged by size: 1712 1443 1105 1103 394 274 241 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6534 6566 6534 Size: 32 6542 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6574 XbaI T'CTAG_A Cuts at: 3910 3910 Size: 6574 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6574 XmaI C'CCGG_G Cuts at: 1870 3899 1870 Size: 2029 4545 XmnI GAAnn'nnTTC Cuts at: 2583 4703 2583 Size: 2120 4454 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4382 276 Size: 53 83 186 936 2848 2468 Fragments arranged by size: 2848 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PacI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PfoI PmlI PspOMI PspXI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI XcmI