Restriction map for
pFN21AA1485
(Circular) (Six-base) MAPSORT of: pFN21AA1485.seq Check: 2165 from: 1 to: 8434
pFN21AA1485 8434 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2964 3014 4008 2964
Size: 50 994 7390
Fragments arranged by size:
7390 994 50
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3102 6244 278
Size: 53 83 186 936 1566 3142 2468
Fragments arranged by size:
3142 2468 1566 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5634 5670 5777 1937
Size: 3697 36 107 4594
Fragments arranged by size:
4594 3697 107 36
Acc65I G'GTAC_C
Cuts at: 4260 5755 4260
Size: 1495 6939
AclI AA'CG_TT
Cuts at: 1563 6561 6934 1563
Size: 4998 373 3063
Fragments arranged by size:
4998 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2691 4418 4676 5051 6497 7881 8149 1632
Size: 1059 1727 258 375 1446 1384 268 1917
Fragments arranged by size:
1917 1727 1446 1384 1059 375 268 258
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8237
AflIII A'CryG_T
Cuts at: 1203 4824 7333 8310 1203
Size: 3621 2509 977 1327
Fragments arranged by size:
3621 2509 1327 977
AgeI A'CCGG_T
Cuts at: 4128 7265 4128
Size: 3137 5297
AhdI GACnn_n'nnGTC
Cuts at: 7163 7163
Size: 8434
AleI CACnn'nnGTG
Cuts at: 1166 3228 4818 5387 1166
Size: 2062 1590 569 4213
Fragments arranged by size:
4213 2062 1590 569
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2435 3468 3734 1184
Size: 1251 1033 266 5884
Fragments arranged by size:
5884 1251 1033 266
AlwNI CAG_nnn'CTG
Cuts at: 3343 4070 4948 5344 6177 7749 3343
Size: 727 878 396 833 1572 4028
Fragments arranged by size:
4028 1572 878 833 727 396
ApaI G_GGCC'C
Cuts at: 2673 2673
Size: 8434
ApaLI G'TGCA_C
Cuts at: 2951 3032 3047 3443 3683 4906 6491 7647 2951
Size: 81 15 396 240 1223 1585 1156 3738
Fragments arranged by size:
3738 1585 1223 1156 396 240 81 15
ApoI r'AATT_y
Cuts at: 1493 2425 2692 4838 5743 5969 6121 8230 1493
Size: 932 267 2146 905 226 152 2109 1697
Fragments arranged by size:
2146 2109 1697 932 905 267 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6988 160
Size: 872 5956 1606
Fragments arranged by size:
5956 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8434
AvaI C'yCGr_G
Cuts at: 1870 1943 4299 4598 5759 1870
Size: 73 2356 299 1161 4545
Fragments arranged by size:
4545 2356 1161 299 73
AvrII C'CTAG_G
Cuts at: 3964 3964
Size: 8434
BamHI G'GATC_C
Cuts at: 2877 4206 5764 6131 2877
Size: 1329 1558 367 5180
Fragments arranged by size:
5180 1558 1329 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2719 3950 4260 5081 5156
Size: 325 217 627 932 1231 310 821 75
Cuts at: 5156 5420 5467 5755 7210 618
Size: 264 47 288 1455 1842
Fragments arranged by size:
1842 1455 1231 932 821 627 325 310
288 264 217 75 47
BanII G_rGCy'C
Cuts at: 1391 2673 3177 3312 3962 4711 5265 5514 5753
Size: 1282 504 135 650 749 554 249 239
Cuts at: 5753 1391
Size: 4072
Fragments arranged by size:
4072 1282 749 650 554 504 249 239
135
BbeI G_GCGC'C
Cuts at: 3954 5424 3954
Size: 1470 6964
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2833 3463 5307 928
Size: 971 934 630 1844 4055
Fragments arranged by size:
4055 1844 971 934 630
BbvCI CC'TCA_GC
Cuts at: 4041 4041
Size: 8434
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3385 3419 6625 6659 1903
Size: 34 1448 34 3206 34 3678
Fragments arranged by size:
3678 3206 1448 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6326 7536 6326
Size: 1210 7224
BclI T'GATC_A
Cuts at: 1548 3894 1548
Size: 2346 6088
BfrBI ATG'CAT
Cuts at: 4282 4282
Size: 8434
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2936 3461 7045
Size: 107 122 71 631 605 1264 525 3584
Cuts at: 7045 136
Size: 1525
Fragments arranged by size:
3584 1525 1264 631 605 525 122 107
71
BglII A'GATC_T
Cuts at: 2553 8429 2553
Size: 5876 2558
BlpI GC'TnA_GC
Cuts at: 5851 5851
Size: 8434
Bme1580I G_kGCm'C
Cuts at: 1790 2673 2909 2955 3036 3051 3447 3687 4910
Size: 883 236 46 81 15 396 240 1223
Cuts at: 4910 4996 5084 6495 7651 1790
Size: 86 88 1411 1156 2573
Fragments arranged by size:
2573 1411 1223 1156 883 396 240 236
88 86 81 46 15
BmgBI CAC'GTC
Cuts at: 1318 5608 1318
Size: 4290 4144
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2905 5266 6165
Size: 416 53 83 399 24 1488 2361 899
Cuts at: 6165 7123 442
Size: 958 1753
Fragments arranged by size:
2361 1753 1488 958 899 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8434
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2323 5281 5533 2323
Size: 2958 252 5224
Fragments arranged by size:
5224 2958 252
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2991 3563 3650 4047 4250 4416 5321 7094
Size: 1752 572 87 397 203 166 905 1773
Cuts at: 7094 1239
Size: 2579
Fragments arranged by size:
2579 1773 1752 905 572 397 203 166
87
Bpu10I CC'TnA_GC
Cuts at: 3011 4041 4122 3011
Size: 1030 81 7323
Fragments arranged by size:
7323 1030 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2167 3011 3581 3756 4760 6559 7424 7722 2167
Size: 844 570 175 1004 1799 865 298 2879
Fragments arranged by size:
2879 1799 1004 865 844 570 298 175
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2693 3754 4218 4576 5217 5420 7097
Size: 617 1194 1061 464 358 641 203 1677
Cuts at: 7097 882
Size: 2219
Fragments arranged by size:
2219 1677 1194 1061 641 617 464 358
203
BsaAI yAC'GTr
Cuts at: 493 1204 4369 5674 6225 493
Size: 711 3165 1305 551 2702
Fragments arranged by size:
3165 2702 1305 711 551
BsaBI GATnn'nnATC
Cuts at: 8428 8428
Size: 8434
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3099 3951 5421 5436
Size: 53 83 186 936 1566 852 1470 15
Cuts at: 5436 6241 6623 7258 275
Size: 805 382 635 1451
Fragments arranged by size:
1566 1470 1451 936 852 805 635 382
186 83 53 15
BsaWI w'CCGG_w
Cuts at: 2192 2317 4128 4209 6867 7265 7539 7686 8097
Size: 125 1811 81 2658 398 274 147 411
Cuts at: 8097 2192
Size: 2529
Fragments arranged by size:
2658 2529 1811 411 398 274 147 125
81
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3677 3707 5236 5266 1178
Size: 30 165 30 2274 30 1529 30 4346
Fragments arranged by size:
4346 2274 1529 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2075 2282 2450 3581 3647 3742 3745 3805
Size: 889 207 168 1131 66 95 3 60
Cuts at: 3805 4046 4618 5078 5273 5519 5522 5714 1186
Size: 241 572 460 195 246 3 192 3906
Fragments arranged by size:
3906 1131 889 572 460 246 241 207
195 192 168 95 66 60 3 3
BseYI C'CCAG_C
Cuts at: 2666 3676 3926 4013 4036 5416 5513 7637 8133
Size: 1010 250 87 23 1380 97 2124 496
Cuts at: 8133 2666
Size: 2967
Fragments arranged by size:
2967 2124 1380 1010 496 250 97 87
23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2604 2935 5696 1163
Size: 573 868 331 2761 3901
Fragments arranged by size:
3901 2761 868 573 331
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5371 5884 6645 7673 1540
Size: 275 186 3370 513 761 1028 2301
Fragments arranged by size:
3370 2301 1028 761 513 275 186
BsiHKAI G_wGCw'C
Cuts at: 2955 3036 3051 3177 3312 3447 3687 4128 4583
Size: 81 15 126 135 135 240 441 455
Cuts at: 4583 4910 5109 6495 6580 7651 2955
Size: 327 199 1386 85 1071 3738
Fragments arranged by size:
3738 1386 1071 455 441 327 240 199
135 135 126 85 81 15
BsmI GAATG_Cn'
Cuts at: 3595 3766 5945 6038 3595
Size: 171 2179 93 5991
Fragments arranged by size:
5991 2179 171 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2003 3120 5537 5615 2003
Size: 1117 2417 78 4822
Fragments arranged by size:
4822 2417 1117 78
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2673 2909 2955 3036 3051 3177 3312
Size: 399 883 236 46 81 15 126 135
Cuts at: 3312 3447 3687 3962 4128 4583 4711 4910 4996
Size: 135 240 275 166 455 128 199 86
Cuts at: 4996 5084 5109 5265 5514 5753 6495 6580 7651
Size: 88 25 156 249 239 742 85 1071
Cuts at: 7651 1391
Size: 2174
Fragments arranged by size:
2174 1071 883 742 455 399 275 249
240 239 236 199 166 156 135 135
128 126 88 86 85 81 46 25
15
BspEI T'CCGG_A
Cuts at: 2317 4209 8097 2317
Size: 1892 3888 2654
Fragments arranged by size:
3888 2654 1892
BspHI T'CATG_A
Cuts at: 2211 4149 4671 4711 5532 6323 8053 2211
Size: 1938 522 40 821 791 1730 2592
Fragments arranged by size:
2592 1938 1730 821 791 522 40
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2441 2964 3014 3771 4008 5485 5789 844
Size: 1597 523 50 757 237 1477 304 3489
Fragments arranged by size:
3489 1597 1477 757 523 304 237 50
BsrBI CCG'CTC
Cuts at: 1394 1823 4204 6321 1394
Size: 429 2381 2117 3507
Fragments arranged by size:
3507 2381 2117 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4996 6929 7103 65
Size: 1183 3748 1933 174 1396
Fragments arranged by size:
3748 1933 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4128 5719 7078 7265 1700
Size: 110 2318 1591 1359 187 2869
Fragments arranged by size:
2869 2318 1591 1359 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8434
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8434
BssSI C'ACGA_G
Cuts at: 6494 7506 6494
Size: 1012 7422
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2860 794
Size: 2066 6368
BstBI TT'CG_AA
Cuts at: 7238 7238
Size: 8434
BstEII G'GTnAC_C
Cuts at: 1184 4001 5497 1184
Size: 2817 1496 4121
Fragments arranged by size:
4121 2817 1496
BstXI CCAn_nnnn'nTGG
Cuts at: 3926 5120 3926
Size: 1194 7240
BstYI r'GATC_y
Cuts at: 1972 2553 2877 4009 4206 5764 6131 6516 6533
Size: 581 324 1132 197 1558 367 385 17
Cuts at: 6533 7985 8071 8083 8429 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1558 1452 1132 581 385 367 346
324 197 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2150 2235 2873 4566 5256 2150
Size: 85 638 1693 690 5328
Fragments arranged by size:
5328 1693 690 638 85
BtgI C'CryG_G
Cuts at: 513 1065 2004 2522 3280 3319 3744 3946 5382
Size: 552 939 518 758 39 425 202 1436
Cuts at: 5382 5463 5562 513
Size: 81 99 3385
Fragments arranged by size:
3385 1436 939 758 552 518 425 202
99 81 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7695
Fragments arranged by size:
7695 683 56
BtsI GCAGTG_nn'
Cuts at: 3067 4881 5439 5950 6744 6764 3067
Size: 1814 558 511 794 20 4737
Fragments arranged by size:
4737 1814 794 558 511 20
ClaI AT'CG_AT
Cuts at: 1554 4531 1554
Size: 2977 5457
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5428 5463 529
Size: 35 4864 35 3500
Fragments arranged by size:
4864 3500 35 35
DraI TTT'AAA
Cuts at: 5738 5738
Size: 8434
DraIII CAC_nnn'GTG
Cuts at: 3728 4914 5590 3728
Size: 1186 676 6572
Fragments arranged by size:
6572 1186 676
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3152 7441 809
Size: 507 1836 4289 1802
Fragments arranged by size:
4289 1836 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2031 3316 5328
Size: 54 1425 50 275 18 201 1285 2012
Cuts at: 5328 5881 6770 8399 8
Size: 553 889 1629 43
Fragments arranged by size:
2012 1629 1425 1285 889 553 275 201
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5881 1537
Size: 275 4069 4090
Fragments arranged by size:
4090 4069 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2066 2210 2216 2259 3558 3597 6364 1353
Size: 713 144 6 43 1299 39 2767 3423
Fragments arranged by size:
3423 2767 1299 713 144 43 39 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7021 7395 7541 387
Size: 1064 138 5432 374 146 1280
Fragments arranged by size:
5432 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3175 3310 3175
Size: 135 8299
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2691 2991 3563 3650 4047 4250 4416
Size: 393 1059 300 572 87 397 203 166
Cuts at: 4416 4418 4676 5051 5321 6497 7094 7881 8149
Size: 2 258 375 270 1176 597 787 268
Cuts at: 8149 1239
Size: 1524
Fragments arranged by size:
1524 1176 1059 787 597 572 397 393
375 300 270 268 258 203 166 87
2
EcoNI CCTnn'n_nnAGG
Cuts at: 2679 2727 3014 2679
Size: 48 287 8099
Fragments arranged by size:
8099 287 48
EcoO109I rG'GnC_Cy
Cuts at: 2670 2795 3655 4156 5341 2670
Size: 125 860 501 1185 5763
Fragments arranged by size:
5763 1185 860 501 125
EcoRI G'AATT_C
Cuts at: 5743 6121 5743
Size: 378 8056
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3385 3385
Size: 8434
FspI TGC'GCA
Cuts at: 2960 3425 6940 2960
Size: 465 3515 4454
Fragments arranged by size:
4454 3515 465
HaeII r_GCGC'y
Cuts at: 1678 3344 3954 5424 7581 1678
Size: 1666 610 1470 2157 2531
Fragments arranged by size:
2531 2157 1666 1470 610
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2981 3013 3800 3832 4115
Size: 32 135 32 1409 32 787 32 283
Cuts at: 4115 4147 4546 4578 7075 7107 7149 7181 1373
Size: 32 399 32 2497 32 42 32 2626
Fragments arranged by size:
2626 2497 1409 787 399 283 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5778 6024 669
Size: 988 281 3840 246 3079
Fragments arranged by size:
3840 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8434
HpaI GTT'AAC
Cuts at: 6024 6024
Size: 8434
KasI G'GCGC_C
Cuts at: 3950 5420 3950
Size: 1470 6964
KpnI G_GTAC'C
Cuts at: 4264 5759 4264
Size: 1495 6939
MfeI C'AATT_G
Cuts at: 6033 6033
Size: 8434
MluI A'CGCG_T
Cuts at: 8310 8310
Size: 8434
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2058 2318 2351 2406 5544 5625 7548 7732
Size: 753 260 33 55 3138 81 1923 184
Cuts at: 7732 1305
Size: 2007
Fragments arranged by size:
3138 2007 1923 753 260 184 81 55
33
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3318 10
Size: 54 1425 343 1486 5126
Fragments arranged by size:
5126 1486 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2039 2417 2606 2886 2902 3228 3986
Size: 648 873 378 189 280 16 326 758
Cuts at: 3986 4716 4818 5058 5387 5531 6392 6751 6910
Size: 730 102 240 329 144 861 359 159
Cuts at: 6910 518
Size: 2042
Fragments arranged by size:
2042 873 861 758 730 648 378 359
329 326 280 240 189 159 144 102
16
MspA1I CmG'CkG
Cuts at: 1606 2395 3208 3556 3628 3680 3998 4040 4615
Size: 789 813 348 72 52 318 42 575
Cuts at: 4615 4928 4945 4989 5099 5324 5850 6527 7675
Size: 313 17 44 110 225 526 677 1148
Cuts at: 7675 7920 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 813 789 677 575 526 348
318 313 245 225 110 72 52 44
42 17
NaeI GCC'GGC
Cuts at: 5721 5721
Size: 8434
NarI GG'CG_CC
Cuts at: 3951 5421 3951
Size: 1470 6964
NcoI C'CATG_G
Cuts at: 513 1065 2004 3319 3946 5463 513
Size: 552 939 1315 627 1517 3484
Fragments arranged by size:
3484 1517 1315 939 627 552
NdeI CA'TA_TG
Cuts at: 387 3270 4785 387
Size: 2883 1515 4036
Fragments arranged by size:
4036 2883 1515
NgoMIV G'CCGG_C
Cuts at: 5719 5719
Size: 8434
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8434
NotI GC'GGCC_GC
Cuts at: 5881 5881
Size: 8434
NsiI A_TGCA'T
Cuts at: 4284 4284
Size: 8434
NspI r_CATG'y
Cuts at: 1132 1229 3356 3859 4286 4828 4970 5792 7337
Size: 97 2127 503 427 542 142 822 1545
Cuts at: 7337 1132
Size: 2229
Fragments arranged by size:
2229 2127 1545 822 542 503 427 142
97
PasI CC'CwG_GG
Cuts at: 1348 3659 1348
Size: 2311 6123
PciI A'CATG_T
Cuts at: 4824 7333 4824
Size: 2509 5925
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3274 3343 4070 5590 1108
Size: 2166 69 727 1520 3952
Fragments arranged by size:
3952 2166 1520 727 69
PfoI T'CCnGG_A
Cuts at: 3504 5337 5538 3504
Size: 1833 201 6400
Fragments arranged by size:
6400 1833 201
PmeI GTTT'AAAC
Cuts at: 5738 5738
Size: 8434
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3224 3256 6503 6535 8016 8048 3224
Size: 32 3247 32 1481 32 3610
Fragments arranged by size:
3610 3247 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2795 4156 5341 2795
Size: 1361 1185 5888
Fragments arranged by size:
5888 1361 1185
PshAI GACnn'nnGTC
Cuts at: 1613 2524 1613
Size: 911 7523
PsiI TTA'TAA
Cuts at: 6004 6004
Size: 8434
PspOMI G'GGCC_C
Cuts at: 2669 2669
Size: 8434
PstI C_TGCA'G
Cuts at: 830 2865 4888 5403 5786 830
Size: 2035 2023 515 383 3478
Fragments arranged by size:
3478 2035 2023 515 383
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8434
PvuII CAG'CTG
Cuts at: 2395 3208 3556 3628 3680 3998 4040 4615 4945
Size: 813 348 72 52 318 42 575 330
Cuts at: 4945 2395
Size: 5884
Fragments arranged by size:
5884 813 575 348 330 318 72 52
42
SacI G_AGCT'C
Cuts at: 3177 3312 3177
Size: 135 8299
SalI G'TCGA_C
Cuts at: 1936 5776 1936
Size: 3840 4594
SbfI CC_TGCA'GG
Cuts at: 5786 5786
Size: 8434
ScaI AGT'ACT
Cuts at: 3092 6682 8394 3092
Size: 3590 1712 3132
Fragments arranged by size:
3590 3132 1712
SexAI A'CCwGG_T
Cuts at: 2715 3511 5586 2715
Size: 796 2075 5563
Fragments arranged by size:
5563 2075 796
SfcI C'TryA_G
Cuts at: 826 1045 2861 3551 4448 4884 5399 5782 6917
Size: 219 1816 690 897 436 515 383 1135
Cuts at: 6917 7598 7789 826
Size: 681 191 1471
Fragments arranged by size:
1816 1471 1135 897 690 681 515 436
383 219 191
SfoI GGC'GCC
Cuts at: 3952 5422 3952
Size: 1470 6964
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8434
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8434
SmaI CCC'GGG
Cuts at: 1872 4301 5761 1872
Size: 2429 1460 4545
Fragments arranged by size:
4545 2429 1460
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2182 3026 3596 3771 4775 6538
Size: 197 926 239 844 570 175 1004 1763
Cuts at: 6538 7439 7701 820
Size: 901 262 1553
Fragments arranged by size:
1763 1553 1004 926 901 844 570 262
239 197 175
SnaBI TAC'GTA
Cuts at: 493 6225 493
Size: 5732 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8434
SphI G_CATG'C
Cuts at: 1132 5792 1132
Size: 4660 3774
SspI AAT'ATT
Cuts at: 5 52 6358 5
Size: 47 6306 2081
Fragments arranged by size:
6306 2081 47
StuI AGG'CCT
Cuts at: 1517 2155 2233 3164 3464 3608 4046 5654 1517
Size: 638 78 931 300 144 438 1608 4297
Fragments arranged by size:
4297 1608 931 638 438 300 144 78
StyI C'CwwG_G
Cuts at: 513 1065 2004 3165 3319 3465 3912 3946 3964
Size: 552 939 1161 154 146 447 34 18
Cuts at: 3964 5463 5873 513
Size: 1499 410 3074
Fragments arranged by size:
3074 1499 1161 939 552 447 410 154
146 34 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2420 3443 4307 5285 5385 6461 6478
Size: 1255 840 1023 864 978 100 1076 17
Cuts at: 6478 6631 6816 7261 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1076 1023 978 864 840 445
185 153 100 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2162 3090 6680
Size: 274 80 33 51 1443 185 928 3590
Cuts at: 6680 8392 96
Size: 1712 138
Fragments arranged by size:
3590 1712 1443 928 274 185 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3677 3709 8394 8426 3677
Size: 32 4685 32 3685
Fragments arranged by size:
4685 3685 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8434
XbaI T'CTAG_A
Cuts at: 5770 5770
Size: 8434
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2605 5692 2605
Size: 3087 5347
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8434
XmaI C'CCGG_G
Cuts at: 1870 4299 5759 1870
Size: 2429 1460 4545
Fragments arranged by size:
4545 2429 1460
XmnI GAAnn'nnTTC
Cuts at: 2631 4727 5304 6563 2631
Size: 2096 577 1259 4502
Fragments arranged by size:
4502 2096 1259 577
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3100 6242 276
Size: 53 83 186 936 1566 3142 2468
Fragments arranged by size:
3142 2468 1566 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BsiWI BstZ17I EcoRV FseI
FspAI NruI PacI PmlI PspXI PsrI RsrII SacII
SanDI SapI SfiI SrfI SwaI