Restriction map for
pFN21AA1480
(Circular) (Six-base) MAPSORT of: pFN21AA1480.seq Check: 5599 from: 1 to: 7129
pFN21AA1480 7129 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3919 3919
Size: 7129
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4939 278
Size: 53 83 186 936 3403 2468
Fragments arranged by size:
3403 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2496 3257 4472 1937
Size: 559 761 1215 4594
Fragments arranged by size:
4594 1215 761 559
Acc65I G'GTAC_C
Cuts at: 4450 4450
Size: 7129
AclI AA'CG_TT
Cuts at: 1563 2251 4050 5256 5629 1563
Size: 688 1799 1206 373 3063
Fragments arranged by size:
3063 1799 1206 688 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 5192 6576 6844 1632
Size: 3560 1384 268 1917
Fragments arranged by size:
3560 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6932
AflIII A'CryG_T
Cuts at: 1203 3792 6028 7005 1203
Size: 2589 2236 977 1327
Fragments arranged by size:
2589 2236 1327 977
AgeI A'CCGG_T
Cuts at: 5960 5960
Size: 7129
AhdI GACnn_n'nnGTC
Cuts at: 5858 5858
Size: 7129
AleI CACnn'nnGTG
Cuts at: 1166 2342 3743 3821 4206 1166
Size: 1176 1401 78 385 4089
Fragments arranged by size:
4089 1401 1176 385 78
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2967 1184
Size: 1783 5346
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3605 3637 3605
Size: 32 7097
AlwNI CAG_nnn'CTG
Cuts at: 4872 6444 4872
Size: 1572 5557
ApaI G_GGCC'C
Cuts at: 4141 4193 4141
Size: 52 7077
ApaLI G'TGCA_C
Cuts at: 5186 6342 5186
Size: 1156 5973
ApoI r'AATT_y
Cuts at: 1493 4438 4664 4816 6925 1493
Size: 2945 226 152 2109 1697
Fragments arranged by size:
2945 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5683 160
Size: 872 4651 1606
Fragments arranged by size:
4651 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7129
AvaI C'yCGr_G
Cuts at: 1870 1943 2166 2743 3678 4422 4454 1870
Size: 73 223 577 935 744 32 4545
Fragments arranged by size:
4545 935 744 577 223 73 32
BamHI G'GATC_C
Cuts at: 3589 4459 4826 3589
Size: 870 367 5892
Fragments arranged by size:
5892 870 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2162 2218 3766 4092 4450
Size: 325 217 627 375 56 1548 326 358
Cuts at: 4450 5905 618
Size: 1455 1842
Fragments arranged by size:
1842 1548 1455 627 375 358 326 325
217 56
BanII G_rGCy'C
Cuts at: 1391 2045 2415 2658 3342 4104 4141 4193 4448
Size: 654 370 243 684 762 37 52 255
Cuts at: 4448 1391
Size: 4072
Fragments arranged by size:
4072 762 684 654 370 255 243 52
37
BbeI G_GCGC'C
Cuts at: 4096 4096
Size: 7129
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2427 2930 928
Size: 971 528 503 5127
Fragments arranged by size:
5127 971 528 503
BbvCI CC'TCA_GC
Cuts at: 2102 4122 2102
Size: 2020 5109
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2536 2557 2570 2591 2681 2715 3347
Size: 34 599 21 13 21 90 34 632
Cuts at: 3347 3381 5320 5354 1903
Size: 34 1939 34 3678
Fragments arranged by size:
3678 1939 632 599 90 34 34 34
34 21 21 13
BciVI GTATCCnnnnn_n'
Cuts at: 3829 5021 6231 3829
Size: 1192 1210 4727
Fragments arranged by size:
4727 1210 1192
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7129
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4028 4108 5740
Size: 107 122 71 631 605 2356 80 1632
Cuts at: 5740 136
Size: 1525
Fragments arranged by size:
2356 1632 1525 631 605 122 107 80
71
BglII A'GATC_T
Cuts at: 7124 7124
Size: 7129
BlpI GC'TnA_GC
Cuts at: 4546 4546
Size: 7129
Bme1580I G_kGCm'C
Cuts at: 1790 2167 2223 2324 2349 3771 4141 4193 5190
Size: 377 56 101 25 1422 370 52 997
Cuts at: 5190 6346 1790
Size: 1156 2573
Fragments arranged by size:
2573 1422 1156 997 377 370 101 56
52 25
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7129
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2175 2309 3274
Size: 416 53 83 399 24 758 134 965
Cuts at: 3274 3594 4196 4860 5818 442
Size: 320 602 664 958 1753
Fragments arranged by size:
1753 965 958 758 664 602 416 399
320 134 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7129
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3704 3704
Size: 7129
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2646 3904 4143 5789 1239
Size: 1407 1258 239 1646 2579
Fragments arranged by size:
2579 1646 1407 1258 239
Bpu10I CC'TnA_GC
Cuts at: 2039 2102 2156 4122 2039
Size: 63 54 1966 5046
Fragments arranged by size:
5046 1966 63 54
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3101 5254 6119 6417 3101
Size: 2153 865 298 3813
Fragments arranged by size:
3813 2153 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2709 3119 3233 3281 3675 5792 882
Size: 617 1210 410 114 48 394 2117 2219
Fragments arranged by size:
2219 2117 1210 617 410 394 114 48
BsaAI yAC'GTr
Cuts at: 493 1204 2998 4920 493
Size: 711 1794 1922 2702
Fragments arranged by size:
2702 1922 1794 711
BsaBI GATnn'nnATC
Cuts at: 7123 7123
Size: 7129
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4093 4936 5318 5953
Size: 53 83 186 936 2560 843 382 635
Cuts at: 5953 275
Size: 1451
Fragments arranged by size:
2560 1451 936 843 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2292 3832 5562 5960 6234 6381 6792 2292
Size: 1540 1730 398 274 147 411 2629
Fragments arranged by size:
2629 1730 1540 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4397 4427 1178
Size: 30 165 30 2994 30 3880
Fragments arranged by size:
3880 2994 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2762 4178 4181 1186
Size: 1576 1416 3 4134
Fragments arranged by size:
4134 1576 1416 3
BseYI C'CCAG_C
Cuts at: 2122 2682 2844 6332 6828 2122
Size: 560 162 3488 496 2423
Fragments arranged by size:
3488 2423 560 496 162
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6556
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2241 4579 5340 6368 1540
Size: 275 186 240 2338 761 1028 2301
Fragments arranged by size:
2338 2301 1028 761 275 240 186
BsiHKAI G_wGCw'C
Cuts at: 3890 5190 5275 6346 3890
Size: 1300 85 1071 4673
Fragments arranged by size:
4673 1300 1071 85
BsmI GAATG_Cn'
Cuts at: 3934 4640 4733 3934
Size: 706 93 6330
Fragments arranged by size:
6330 706 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3187 3187
Size: 7129
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2045 2167 2223 2324 2349 2415 2658
Size: 399 255 122 56 101 25 66 243
Cuts at: 2658 3342 3771 3890 4104 4141 4193 4448 5190
Size: 684 429 119 214 37 52 255 742
Cuts at: 5190 5275 6346 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 684 429 399 255 255
243 214 122 119 101 85 66 56
52 37 25
BspEI T'CCGG_A
Cuts at: 2292 3832 6792 2292
Size: 1540 2960 2629
Fragments arranged by size:
2960 2629 1540
BspHI T'CATG_A
Cuts at: 3423 3555 4299 5018 6748 3423
Size: 132 744 719 1730 3804
Fragments arranged by size:
3804 1730 744 719 132
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3792 3919 4484 844
Size: 2948 127 565 3489
Fragments arranged by size:
3489 2948 565 127
BsrBI CCG'CTC
Cuts at: 1394 1823 5016 1394
Size: 429 3193 3507
Fragments arranged by size:
3507 3193 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2575 4247 4352 5624 5798 65
Size: 1183 1327 1672 105 1272 174 1396
Fragments arranged by size:
1672 1396 1327 1272 1183 174 105
BsrFI r'CCGG_y
Cuts at: 1700 1810 2362 3601 4214 5773 5960 1700
Size: 110 552 1239 613 1559 187 2869
Fragments arranged by size:
2869 1559 1239 613 552 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7129
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7129
BssSI C'ACGA_G
Cuts at: 3987 4202 5189 6201 3987
Size: 215 987 1012 4915
Fragments arranged by size:
4915 1012 987 215
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4354 794
Size: 3560 3569
BstBI TT'CG_AA
Cuts at: 5933 5933
Size: 7129
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7129
BstXI CCAn_nnnn'nTGG
Cuts at: 2884 2962 3193 3745 4024 4336 2884
Size: 78 231 552 279 312 5677
Fragments arranged by size:
5677 552 312 279 231 78
BstYI r'GATC_y
Cuts at: 1972 2187 2667 3048 3589 4459 4826 5211 5228
Size: 215 480 381 541 870 367 385 17
Cuts at: 5228 6680 6766 6778 7124 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 870 541 480 385 381 367
346 215 86 17 12
BstZ17I GTA'TAC
Cuts at: 3258 3258
Size: 7129
Bsu36I CC'TnA_GG
Cuts at: 3144 3216 3144
Size: 72 7057
BtgI C'CryG_G
Cuts at: 513 1065 2052 2503 2919 4023 513
Size: 552 987 451 416 1104 3619
Fragments arranged by size:
3619 1104 987 552 451 416
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4003 487
Size: 683 56 2777 3613
Fragments arranged by size:
3613 2777 683 56
BtsI GCAGTG_nn'
Cuts at: 2174 2373 3409 4242 4645 5439 5459 2174
Size: 199 1036 833 403 794 20 3844
Fragments arranged by size:
3844 1036 833 794 403 199 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7129
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7094
DraI TTT'AAA
Cuts at: 4433 4433
Size: 7129
DraIII CAC_nnn'GTG
Cuts at: 2082 2082
Size: 7129
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3179 6136 809
Size: 507 1863 2957 1802
Fragments arranged by size:
2957 1863 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3896 4576 5465
Size: 54 1425 50 275 18 2066 680 889
Cuts at: 5465 7094 8
Size: 1629 43
Fragments arranged by size:
2066 1629 1425 889 680 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4576 1537
Size: 275 2764 4090
Fragments arranged by size:
4090 2764 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3320 5059 1353
Size: 1967 1739 3423
Fragments arranged by size:
3423 1967 1739
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5716 6090 6236 387
Size: 1064 138 4127 374 146 1280
Fragments arranged by size:
4127 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2646 3904 4143 5192 5789 6576 6844
Size: 393 1014 1258 239 1049 597 787 268
Cuts at: 6844 1239
Size: 1524
Fragments arranged by size:
1524 1258 1049 1014 787 597 393 268
239
EcoNI CCTnn'n_nnAGG
Cuts at: 3489 3489
Size: 7129
EcoO109I rG'GnC_Cy
Cuts at: 2119 2195 2674 3494 3939 4029 4137 4138 4190
Size: 76 479 820 445 90 108 1 52
Cuts at: 4190 2119
Size: 5058
Fragments arranged by size:
5058 820 479 445 108 90 76 52
1
EcoRI G'AATT_C
Cuts at: 4438 4816 4438
Size: 378 6751
EcoRV GAT'ATC
Cuts at: 2389 2389
Size: 7129
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3766 3766
Size: 7129
FspI TGC'GCA
Cuts at: 5635 5635
Size: 7129
HaeII r_GCGC'y
Cuts at: 1678 2102 4096 4279 6276 1678
Size: 424 1994 183 1997 2531
Fragments arranged by size:
2531 1997 1994 424 183
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2802 2834 3140 3172 3228
Size: 32 135 32 1230 32 306 32 56
Cuts at: 3228 3260 3343 3375 3574 3606 5770 5802 5844
Size: 32 83 32 199 32 2164 32 42
Cuts at: 5844 5876 1373
Size: 32 2626
Fragments arranged by size:
2626 2164 1230 306 199 135 83 56
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2137 3100 4473 4719 669
Size: 988 281 199 963 1373 246 3079
Fragments arranged by size:
3079 1373 988 963 281 246 199
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7129
HpaI GTT'AAC
Cuts at: 4719 4719
Size: 7129
KasI G'GCGC_C
Cuts at: 4092 4092
Size: 7129
KpnI G_GTAC'C
Cuts at: 4454 4454
Size: 7129
MfeI C'AATT_G
Cuts at: 4728 4728
Size: 7129
MluI A'CGCG_T
Cuts at: 7005 7005
Size: 7129
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2581 6243 6427 1305
Size: 1276 3662 184 2007
Fragments arranged by size:
3662 2007 1276 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5307
Fragments arranged by size:
5307 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2342 3456 3743 3821 4022 4206 4319
Size: 648 1176 1114 287 78 201 184 113
Cuts at: 4319 5087 5446 5605 518
Size: 768 359 159 2042
Fragments arranged by size:
2042 1176 1114 768 648 359 287 201
184 159 113 78
MspA1I CmG'CkG
Cuts at: 1606 2342 2682 2844 4126 4545 5222 6370 6615
Size: 736 340 162 1282 419 677 1148 245
Cuts at: 6615 1606
Size: 2120
Fragments arranged by size:
2120 1282 1148 736 677 419 340 245
162
NarI GG'CG_CC
Cuts at: 4093 4093
Size: 7129
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6577
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7129
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7129
NotI GC'GGCC_GC
Cuts at: 4576 4576
Size: 7129
NspI r_CATG'y
Cuts at: 1132 1229 3368 3796 4487 6032 1132
Size: 97 2139 428 691 1545 2229
Fragments arranged by size:
2229 2139 1545 691 428 97
PasI CC'CwG_GG
Cuts at: 1348 3934 1348
Size: 2586 4543
PciI A'CATG_T
Cuts at: 3792 6028 3792
Size: 2236 4893
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2212 3334 1108
Size: 1104 1122 4903
Fragments arranged by size:
4903 1122 1104
PfoI T'CCnGG_A
Cuts at: 2406 2466 3941 2406
Size: 60 1475 5594
Fragments arranged by size:
5594 1475 60
PmeI GTTT'AAAC
Cuts at: 4433 4433
Size: 7129
PmlI CAC'GTG
Cuts at: 2998 2998
Size: 7129
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5198 5230 6711 6743 5198
Size: 32 1481 32 5584
Fragments arranged by size:
5584 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3939 3939
Size: 7129
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7129
PsiI TTA'TAA
Cuts at: 4699 4699
Size: 7129
PspOMI G'GGCC_C
Cuts at: 4137 4189 4137
Size: 52 7077
PstI C_TGCA'G
Cuts at: 830 4350 4481 830
Size: 3520 131 3478
Fragments arranged by size:
3520 3478 131
PvuI CG_AT'CG
Cuts at: 2001 2241 2001
Size: 240 6889
PvuII CAG'CTG
Cuts at: 2342 2844 2342
Size: 502 6627
SalI G'TCGA_C
Cuts at: 1936 4471 1936
Size: 2535 4594
SbfI CC_TGCA'GG
Cuts at: 4481 4481
Size: 7129
ScaI AGT'ACT
Cuts at: 2623 5377 7089 2623
Size: 2754 1712 2663
Fragments arranged by size:
2754 2663 1712
SfcI C'TryA_G
Cuts at: 826 1045 3916 4346 4477 5612 6293 6484 826
Size: 219 2871 430 131 1135 681 191 1471
Fragments arranged by size:
2871 1471 1135 681 430 219 191 131
SfoI GGC'GCC
Cuts at: 4094 4094
Size: 7129
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7129
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7129
SmaI CCC'GGG
Cuts at: 1872 4424 4456 1872
Size: 2552 32 4545
Fragments arranged by size:
4545 2552 32
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3116 5233 6134 6396 820
Size: 197 926 1173 2117 901 262 1553
Fragments arranged by size:
2117 1553 1173 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4920 493
Size: 4427 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7129
SphI G_CATG'C
Cuts at: 1132 3368 4487 1132
Size: 2236 1119 3774
Fragments arranged by size:
3774 2236 1119
SspI AAT'ATT
Cuts at: 5 52 2698 5053 5
Size: 47 2646 2355 2081
Fragments arranged by size:
2646 2355 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7129
StyI C'CwwG_G
Cuts at: 513 1065 2640 2955 3633 3713 4568 513
Size: 552 1575 315 678 80 855 3074
Fragments arranged by size:
3074 1575 855 678 552 315 80
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2143 2674 3322 3857 4211 5156 5173
Size: 1255 563 531 648 535 354 945 17
Cuts at: 5173 5326 5511 5956 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 945 648 563 535 531 445
354 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2621 2871 5375
Size: 274 80 33 51 1443 644 250 2504
Cuts at: 5375 7087 96
Size: 1712 138
Fragments arranged by size:
2504 1712 1443 644 274 250 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2293 2325 3791 3823 4397 4429 7089 7121 2293
Size: 32 1466 32 574 32 2660 32 2301
Fragments arranged by size:
2660 2301 1466 574 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7129
XbaI T'CTAG_A
Cuts at: 4465 4465
Size: 7129
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2188 2277 3002 2188
Size: 89 725 6315
Fragments arranged by size:
6315 725 89
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7129
XmaI C'CCGG_G
Cuts at: 1870 4422 4454 1870
Size: 2552 32 4545
Fragments arranged by size:
4545 2552 32
XmnI GAAnn'nnTTC
Cuts at: 2252 5258 2252
Size: 3006 4123
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4937 276
Size: 53 83 186 936 3403 2468
Fragments arranged by size:
3403 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BsiWI EcoICRI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SrfI
SwaI