Restriction map for
pFN21AA1478
(Circular) (Six-base) MAPSORT of: pFN21AA1478.seq Check: 5373 from: 1 to: 6601
pFN21AA1478 6601 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4411 278
Size: 53 83 186 936 2875 2468
Fragments arranged by size:
2875 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2628 3944 1937
Size: 691 1316 4594
Fragments arranged by size:
4594 1316 691
Acc65I G'GTAC_C
Cuts at: 3922 3922
Size: 6601
AclI AA'CG_TT
Cuts at: 1563 4728 5101 1563
Size: 3165 373 3063
Fragments arranged by size:
3165 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2240 2555 2966 4664 6048 6316 1632
Size: 608 315 411 1698 1384 268 1917
Fragments arranged by size:
1917 1698 1384 608 411 315 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6404
AflIII A'CryG_T
Cuts at: 1203 3664 5500 6477 1203
Size: 2461 1836 977 1327
Fragments arranged by size:
2461 1836 1327 977
AgeI A'CCGG_T
Cuts at: 5432 5432
Size: 6601
AhdI GACnn_n'nnGTC
Cuts at: 3067 5330 3067
Size: 2263 4338
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6601
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6601
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2762 2794 2762
Size: 32 6569
AlwNI CAG_nnn'CTG
Cuts at: 2192 2669 4344 5916 2192
Size: 477 1675 1572 2877
Fragments arranged by size:
2877 1675 1572 477
ApaI G_GGCC'C
Cuts at: 2691 2691
Size: 6601
ApaLI G'TGCA_C
Cuts at: 4658 5814 4658
Size: 1156 5445
ApoI r'AATT_y
Cuts at: 1493 2927 3200 3910 4136 4288 6397 1493
Size: 1434 273 710 226 152 2109 1697
Fragments arranged by size:
2109 1697 1434 710 273 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5155 160
Size: 872 4123 1606
Fragments arranged by size:
4123 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6601
AvaI C'yCGr_G
Cuts at: 1870 1943 3926 1870
Size: 73 1983 4545
Fragments arranged by size:
4545 1983 73
AvrII C'CTAG_G
Cuts at: 3859 3859
Size: 6601
BamHI G'GATC_C
Cuts at: 3931 4298 3931
Size: 367 6234
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3922 5377 618
Size: 325 217 627 2135 1455 1842
Fragments arranged by size:
2135 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2691 3920 1391
Size: 1300 1229 4072
Fragments arranged by size:
4072 1300 1229
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2528 3363 928
Size: 971 629 835 4166
Fragments arranged by size:
4166 971 835 629
BbvCI CC'TCA_GC
Cuts at: 2271 3230 2271
Size: 959 5642
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4792 4826 1903
Size: 34 2855 34 3678
Fragments arranged by size:
3678 2855 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2002 4493 5703 2002
Size: 2491 1210 2900
Fragments arranged by size:
2900 2491 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6601
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3491 5212 136
Size: 107 122 71 631 605 1819 1721 1525
Fragments arranged by size:
1819 1721 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6596 6596
Size: 6601
BlpI GC'TnA_GC
Cuts at: 4018 4018
Size: 6601
Bme1580I G_kGCm'C
Cuts at: 1790 2691 2834 3534 4662 5818 1790
Size: 901 143 700 1128 1156 2573
Fragments arranged by size:
2573 1156 1128 901 700 143
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6601
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3539 3737 4332
Size: 416 53 83 399 24 2122 198 595
Cuts at: 4332 5290 442
Size: 958 1753
Fragments arranged by size:
2122 1753 958 595 416 399 198 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6601
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2339 3471 2339
Size: 1132 5469
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2822 3632 5261 1239
Size: 1583 810 1629 2579
Fragments arranged by size:
2579 1629 1583 810
Bpu10I CC'TnA_GC
Cuts at: 2271 3230 2271
Size: 959 5642
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3746 3881 4726 5591 5889 3746
Size: 135 845 865 298 4458
Fragments arranged by size:
4458 865 845 298 135
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2974 5264 882
Size: 617 1475 2290 2219
Fragments arranged by size:
2290 2219 1475 617
BsaAI yAC'GTr
Cuts at: 493 1204 2231 4392 493
Size: 711 1027 2161 2702
Fragments arranged by size:
2702 2161 1027 711
BsaBI GATnn'nnATC
Cuts at: 6595 6595
Size: 6601
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4408 4790 5425 275
Size: 53 83 186 936 2875 382 635 1451
Fragments arranged by size:
2875 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5034 5432 5706 5853 6264 5034
Size: 398 274 147 411 5371
Fragments arranged by size:
5371 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2762 2792 1178
Size: 30 165 30 1359 30 4987
Fragments arranged by size:
4987 1359 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2375 3218 1186
Size: 1189 843 4569
Fragments arranged by size:
4569 1189 843
BseYI C'CCAG_C
Cuts at: 2155 2417 5804 6300 2155
Size: 262 3387 496 2456
Fragments arranged by size:
3387 2456 496 262
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6028
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4051 4812 5840 1540
Size: 275 186 2050 761 1028 2301
Fragments arranged by size:
2301 2050 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2206 4662 4747 5818 2206
Size: 2456 85 1071 2989
Fragments arranged by size:
2989 2456 1071 85
BsmI GAATG_Cn'
Cuts at: 2346 3009 3057 4112 4205 2346
Size: 663 48 1055 93 4742
Fragments arranged by size:
4742 1055 663 93 48
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2206 2691 2834 3534 3920 4662 4747
Size: 399 416 485 143 700 386 742 85
Cuts at: 4747 5818 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 700 485 416 399 386
143 85
BspEI T'CCGG_A
Cuts at: 6264 6264
Size: 6601
BspHI T'CATG_A
Cuts at: 3432 3630 4490 6220 3432
Size: 198 860 1730 3813
Fragments arranged by size:
3813 1730 860 198
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3956 844
Size: 3112 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4488 1394
Size: 429 2665 3507
Fragments arranged by size:
3507 2665 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2419 5096 5270 65
Size: 1183 1171 2677 174 1396
Fragments arranged by size:
2677 1396 1183 1171 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5245 5432 1700
Size: 110 3435 187 2869
Fragments arranged by size:
3435 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6601
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6601
BssSI C'ACGA_G
Cuts at: 4661 5673 4661
Size: 1012 5589
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2050 794
Size: 1256 5345
BstBI TT'CG_AA
Cuts at: 3283 5405 3283
Size: 2122 4479
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6601
BstXI CCAn_nnnn'nTGG
Cuts at: 3389 3389
Size: 6601
BstYI r'GATC_y
Cuts at: 1972 2170 3159 3931 4298 4683 4700 6152 6238
Size: 198 989 772 367 385 17 1452 86
Cuts at: 6238 6250 6596 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 989 772 385 367 346 198
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5110
Fragments arranged by size:
5110 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5862
Fragments arranged by size:
5862 683 56
BtsI GCAGTG_nn'
Cuts at: 4117 4911 4931 4117
Size: 794 20 5787
Fragments arranged by size:
5787 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6601
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6566
DraI TTT'AAA
Cuts at: 3905 3905
Size: 6601
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5608 809
Size: 507 4292 1802
Fragments arranged by size:
4292 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4048 4937 6566
Size: 54 1425 50 275 18 2218 889 1629
Cuts at: 6566 8
Size: 43
Fragments arranged by size:
2218 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4048 1537
Size: 275 2236 4090
Fragments arranged by size:
4090 2236 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2515 2534 3306 4531 1353
Size: 1162 19 772 1225 3423
Fragments arranged by size:
3423 1225 1162 772 19
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5188 5562 5708 387
Size: 1064 138 3599 374 146 1280
Fragments arranged by size:
3599 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2240 2555 2822 2966 3632 4664 5261
Size: 393 608 315 267 144 666 1032 597
Cuts at: 5261 6048 6316 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1032 787 666 608 597 393 315
268 267 144
EcoO109I rG'GnC_Cy
Cuts at: 3731 3731
Size: 6601
EcoRI G'AATT_C
Cuts at: 3910 4288 3910
Size: 378 6223
EcoRV GAT'ATC
Cuts at: 2470 3247 2470
Size: 777 5824
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3296 3317 3296
Size: 21 6580
FspI TGC'GCA
Cuts at: 5107 5107
Size: 6601
HaeII r_GCGC'y
Cuts at: 1678 2557 3595 5748 1678
Size: 879 1038 2153 2531
Fragments arranged by size:
2531 2153 1038 879
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2354 2386 2492 2524 3053
Size: 32 135 32 782 32 106 32 529
Cuts at: 3053 3085 5242 5274 5316 5348 1373
Size: 32 2157 32 42 32 2626
Fragments arranged by size:
2626 2157 782 529 135 106 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3575 3945 4191 669
Size: 988 281 1637 370 246 3079
Fragments arranged by size:
3079 1637 988 370 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6601
HpaI GTT'AAC
Cuts at: 4191 4191
Size: 6601
KpnI G_GTAC'C
Cuts at: 3926 3926
Size: 6601
MfeI C'AATT_G
Cuts at: 4200 4200
Size: 6601
MluI A'CGCG_T
Cuts at: 6477 6477
Size: 6601
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5715 5899 1305
Size: 4410 184 2007
Fragments arranged by size:
4410 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4779
Fragments arranged by size:
4779 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3101 3635 4559 4918 5077 518
Size: 648 1935 534 924 359 159 2042
Fragments arranged by size:
2042 1935 924 648 534 359 159
MspA1I CmG'CkG
Cuts at: 1606 2308 2800 2994 4017 4694 5842 6087 1606
Size: 702 492 194 1023 677 1148 245 2120
Fragments arranged by size:
2120 1148 1023 702 677 492 245 194
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5110
Fragments arranged by size:
5110 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6601
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6601
NotI GC'GGCC_GC
Cuts at: 4048 4048
Size: 6601
NspI r_CATG'y
Cuts at: 1132 1229 2228 3668 3959 5504 1132
Size: 97 999 1440 291 1545 2229
Fragments arranged by size:
2229 1545 1440 999 291 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6601
PciI A'CATG_T
Cuts at: 3664 5500 3664
Size: 1836 4765
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2155 1108
Size: 1047 5554
PmeI GTTT'AAAC
Cuts at: 3905 3905
Size: 6601
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2762 2794 3784 3816 4670 4702 6183 6215 2762
Size: 32 990 32 854 32 1481 32 3148
Fragments arranged by size:
3148 1481 990 854 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3731 3731
Size: 6601
PshAI GACnn'nnGTC
Cuts at: 1613 2994 1613
Size: 1381 5220
PsiI TTA'TAA
Cuts at: 4171 4171
Size: 6601
PspOMI G'GGCC_C
Cuts at: 2687 2687
Size: 6601
PstI C_TGCA'G
Cuts at: 830 2626 3953 830
Size: 1796 1327 3478
Fragments arranged by size:
3478 1796 1327
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6601
PvuII CAG'CTG
Cuts at: 2308 2800 2308
Size: 492 6109
SalI G'TCGA_C
Cuts at: 1936 3943 1936
Size: 2007 4594
SbfI CC_TGCA'GG
Cuts at: 3953 3953
Size: 6601
ScaI AGT'ACT
Cuts at: 4849 6561 4849
Size: 1712 4889
SfcI C'TryA_G
Cuts at: 826 1045 2622 2661 3259 3949 5084 5765 5956
Size: 219 1577 39 598 690 1135 681 191
Cuts at: 5956 826
Size: 1471
Fragments arranged by size:
1577 1471 1135 690 681 598 219 191
39
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6601
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6601
SmaI CCC'GGG
Cuts at: 1872 3928 1872
Size: 2056 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3761 3896 4705 5606 5868 820
Size: 197 926 1818 135 809 901 262 1553
Fragments arranged by size:
1818 1553 926 901 809 262 197 135
SnaBI TAC'GTA
Cuts at: 493 4392 493
Size: 3899 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6601
SphI G_CATG'C
Cuts at: 1132 2228 3959 1132
Size: 1096 1731 3774
Fragments arranged by size:
3774 1731 1096
SspI AAT'ATT
Cuts at: 5 52 4525 5
Size: 47 4473 2081
Fragments arranged by size:
4473 2081 47
StuI AGG'CCT
Cuts at: 1517 3150 1517
Size: 1633 4968
StyI C'CwwG_G
Cuts at: 513 1065 2004 2765 3579 3859 4040 513
Size: 552 939 761 814 280 181 3074
Fragments arranged by size:
3074 939 814 761 552 280 181
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4628 4645 4798 4983 5428 325
Size: 1255 3048 17 153 185 445 1498
Fragments arranged by size:
3048 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4847 6559 96
Size: 274 80 33 51 1443 2870 1712 138
Fragments arranged by size:
2870 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3780 3812 6561 6593 3780
Size: 32 2749 32 3788
Fragments arranged by size:
3788 2749 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6601
XbaI T'CTAG_A
Cuts at: 3937 3937
Size: 6601
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6601
XmaI C'CCGG_G
Cuts at: 1870 3926 1870
Size: 2056 4545
XmnI GAAnn'nnTTC
Cuts at: 2525 3300 4730 2525
Size: 775 1430 4396
Fragments arranged by size:
4396 1430 775
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4409 276
Size: 53 83 186 936 2875 2468
Fragments arranged by size:
2875 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI BaeI BbeI BfrBI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI