Restriction map for
pFN21AA1472
(Circular) (Six-base) MAPSORT of: pFN21AA1472.seq Check: 2586 from: 1 to: 6694
pFN21AA1472 6694 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4504 278
Size: 53 83 186 936 2968 2468
Fragments arranged by size:
2968 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2498 4037 1937
Size: 561 1539 4594
Fragments arranged by size:
4594 1539 561
Acc65I G'GTAC_C
Cuts at: 4015 4015
Size: 6694
AclI AA'CG_TT
Cuts at: 1563 4821 5194 1563
Size: 3258 373 3063
Fragments arranged by size:
3258 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2879 3107 4757 6141 6409 1632
Size: 1247 228 1650 1384 268 1917
Fragments arranged by size:
1917 1650 1384 1247 268 228
AflII C'TTAA_G
Cuts at: 820 1017 2909 820
Size: 197 1892 4605
Fragments arranged by size:
4605 1892 197
AflIII A'CryG_T
Cuts at: 1203 2754 5593 6570 1203
Size: 1551 2839 977 1327
Fragments arranged by size:
2839 1551 1327 977
AgeI A'CCGG_T
Cuts at: 5525 5525
Size: 6694
AhdI GACnn_n'nnGTC
Cuts at: 2617 5423 2617
Size: 2806 3888
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6694
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6694
AlwNI CAG_nnn'CTG
Cuts at: 2138 2226 2291 3362 3939 4437 6009 2138
Size: 88 65 1071 577 498 1572 2823
Fragments arranged by size:
2823 1572 1071 577 498 88 65
ApaI G_GGCC'C
Cuts at: 2013 2013
Size: 6694
ApaLI G'TGCA_C
Cuts at: 4751 5907 4751
Size: 1156 5538
ApoI r'AATT_y
Cuts at: 1493 2076 4003 4229 4381 6490 1493
Size: 583 1927 226 152 2109 1697
Fragments arranged by size:
2109 1927 1697 583 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5248 160
Size: 872 4216 1606
Fragments arranged by size:
4216 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6694
AvaI C'yCGr_G
Cuts at: 1870 1943 2455 3550 4019 1870
Size: 73 512 1095 469 4545
Fragments arranged by size:
4545 1095 512 469 73
BamHI G'GATC_C
Cuts at: 3904 4024 4391 3904
Size: 120 367 6207
Fragments arranged by size:
6207 367 120
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3481 4015 5470 618
Size: 325 217 627 1694 534 1455 1842
Fragments arranged by size:
1842 1694 1455 627 534 325 217
BanII G_rGCy'C
Cuts at: 1391 2013 2648 3510 4013 1391
Size: 622 635 862 503 4072
Fragments arranged by size:
4072 862 635 622 503
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2282 2511 928
Size: 971 383 229 5111
Fragments arranged by size:
5111 971 383 229
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4885 4919 1903
Size: 34 2948 34 3678
Fragments arranged by size:
3678 2948 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4586 5796 4586
Size: 1210 5484
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6694
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2307 2978 5305
Size: 107 122 71 631 605 635 671 2327
Cuts at: 5305 136
Size: 1525
Fragments arranged by size:
2327 1525 671 635 631 605 122 107
71
BglII A'GATC_T
Cuts at: 6689 6689
Size: 6694
BlpI GC'TnA_GC
Cuts at: 2197 2642 4111 2197
Size: 445 1469 4780
Fragments arranged by size:
4780 1469 445
Bme1580I G_kGCm'C
Cuts at: 1790 2013 3486 4755 5911 1790
Size: 223 1473 1269 1156 2573
Fragments arranged by size:
2573 1473 1269 1156 223
BmgBI CAC'GTC
Cuts at: 1318 3478 1318
Size: 2160 4534
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2658 2968 4425
Size: 416 53 83 399 24 1241 310 1457
Cuts at: 4425 5383 442
Size: 958 1753
Fragments arranged by size:
1753 1457 1241 958 416 399 310 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6694
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3628 3628
Size: 6694
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3131 3392 3428 3518 3832 5354 1239
Size: 1892 261 36 90 314 1522 2579
Fragments arranged by size:
2579 1892 1522 314 261 90 36
Bpu10I CC'TnA_GC
Cuts at: 3932 3932
Size: 6694
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4819 5684 5982 4819
Size: 865 298 5531
Fragments arranged by size:
5531 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3671 5357 882
Size: 617 2172 1686 2219
Fragments arranged by size:
2219 2172 1686 617
BsaAI yAC'GTr
Cuts at: 493 1204 4485 493
Size: 711 3281 2702
Fragments arranged by size:
3281 2702 711
BsaBI GATnn'nnATC
Cuts at: 6688 6688
Size: 6694
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3560 3795 4501 4883
Size: 53 83 186 936 2027 235 706 382
Cuts at: 4883 5518 275
Size: 635 1451
Fragments arranged by size:
2027 1451 936 706 635 382 235 186
83 53
BsaWI w'CCGG_w
Cuts at: 5127 5525 5799 5946 6357 5127
Size: 398 274 147 411 5464
Fragments arranged by size:
5464 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2687 2717 2897 2927 1178
Size: 30 165 30 1284 30 180 30 4945
Fragments arranged by size:
4945 1284 180 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2443 2963 2966 3452 1186
Size: 1257 520 3 486 4428
Fragments arranged by size:
4428 1257 520 486 3
BseYI C'CCAG_C
Cuts at: 2131 2180 2916 3493 5897 6393 2131
Size: 49 736 577 2404 496 2432
Fragments arranged by size:
2432 2404 736 577 496 49
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2063 2650 1163
Size: 573 327 587 5207
Fragments arranged by size:
5207 587 573 327
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4144 4905 5933 1540
Size: 275 186 2143 761 1028 2301
Fragments arranged by size:
2301 2143 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2037 3510 4755 4840 5911 2037
Size: 1473 1245 85 1071 2820
Fragments arranged by size:
2820 1473 1245 1071 85
BsmI GAATG_Cn'
Cuts at: 2379 3066 3382 3878 3958 4205 4298 2379
Size: 687 316 496 80 247 93 4775
Fragments arranged by size:
4775 687 496 316 247 93 80
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2037 2648 3486 3510 4013 4755
Size: 399 223 24 611 838 24 503 742
Cuts at: 4755 4840 5911 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 838 742 611 503 399 223
85 24 24
BspEI T'CCGG_A
Cuts at: 6357 6357
Size: 6694
BspHI T'CATG_A
Cuts at: 2050 2733 3723 4583 6313 2050
Size: 683 990 860 1730 2431
Fragments arranged by size:
2431 1730 990 860 683
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3250 4049 844
Size: 2406 799 3489
Fragments arranged by size:
3489 2406 799
BsrBI CCG'CTC
Cuts at: 1394 1823 2480 4581 1394
Size: 429 657 2101 3507
Fragments arranged by size:
3507 2101 657 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5189 5363 65
Size: 1183 3941 174 1396
Fragments arranged by size:
3941 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5338 5525 1700
Size: 110 3528 187 2869
Fragments arranged by size:
3528 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6694
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6694
BssSI C'ACGA_G
Cuts at: 4754 5766 4754
Size: 1012 5682
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2041 794
Size: 1247 5447
BstBI TT'CG_AA
Cuts at: 5498 5498
Size: 6694
BstEII G'GTnAC_C
Cuts at: 1184 3365 1184
Size: 2181 4513
BstXI CCAn_nnnn'nTGG
Cuts at: 3427 3427
Size: 6694
BstYI r'GATC_y
Cuts at: 1972 3832 3904 4024 4391 4776 4793 6245 6331
Size: 1860 72 120 367 385 17 1452 86
Cuts at: 6331 6343 6689 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1860 1452 385 367 346 120 86
72 17 12
Bsu36I CC'TnA_GG
Cuts at: 2484 2484
Size: 6694
BtgI C'CryG_G
Cuts at: 513 1065 2004 2600 3672 3855 3864 513
Size: 552 939 596 1072 183 9 3343
Fragments arranged by size:
3343 1072 939 596 552 183 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5955
Fragments arranged by size:
5955 683 56
BtsI GCAGTG_nn'
Cuts at: 2813 3451 4210 5004 5024 2813
Size: 638 759 794 20 4483
Fragments arranged by size:
4483 794 759 638 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6694
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6659
DraI TTT'AAA
Cuts at: 3998 3998
Size: 6694
DraIII CAC_nnn'GTG
Cuts at: 2041 2125 3146 2041
Size: 84 1021 5589
Fragments arranged by size:
5589 1021 84
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5701 809
Size: 507 4385 1802
Fragments arranged by size:
4385 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4141 5030 6659
Size: 54 1425 50 275 18 2311 889 1629
Cuts at: 6659 8
Size: 43
Fragments arranged by size:
2311 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4141 1537
Size: 275 2329 4090
Fragments arranged by size:
4090 2329 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2154 3194 3249 3750 4624 1353
Size: 801 1040 55 501 874 3423
Fragments arranged by size:
3423 1040 874 801 501 55
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2566 3956 5281 5655 5801 387
Size: 1064 138 977 1390 1325 374 146 1280
Fragments arranged by size:
1390 1325 1280 1064 977 374 146 138
EcoICRI GAG'CTC
Cuts at: 3508 3508
Size: 6694
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2879 3107 3131 3392 3428 3518 3832
Size: 393 1247 228 24 261 36 90 314
Cuts at: 3832 4757 5354 6141 6409 1239
Size: 925 597 787 268 1524
Fragments arranged by size:
1524 1247 925 787 597 393 314 268
261 228 90 36 24
EcoNI CCTnn'n_nnAGG
Cuts at: 3931 3931
Size: 6694
EcoO109I rG'GnC_Cy
Cuts at: 2009 2010 2540 2611 3538 3924 2009
Size: 1 530 71 927 386 4779
Fragments arranged by size:
4779 927 530 386 71 1
EcoRI G'AATT_C
Cuts at: 2076 4003 4381 2076
Size: 1927 378 4389
Fragments arranged by size:
4389 1927 378
FspI TGC'GCA
Cuts at: 5200 5200
Size: 6694
HaeII r_GCGC'y
Cuts at: 1678 2197 2875 5841 1678
Size: 519 678 2966 2531
Fragments arranged by size:
2966 2531 678 519
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2435 2467 2603 2635 2897
Size: 32 135 32 863 32 136 32 262
Cuts at: 2897 2929 3230 3262 5335 5367 5409 5441 1373
Size: 32 301 32 2073 32 42 32 2626
Fragments arranged by size:
2626 2073 863 301 262 136 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2499 4038 4284 669
Size: 988 281 561 1539 246 3079
Fragments arranged by size:
3079 1539 988 561 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6694
HpaI GTT'AAC
Cuts at: 4284 4284
Size: 6694
KpnI G_GTAC'C
Cuts at: 4019 4019
Size: 6694
MfeI C'AATT_G
Cuts at: 4293 4293
Size: 6694
MluI A'CGCG_T
Cuts at: 6570 6570
Size: 6694
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3744 5808 5992 1305
Size: 2439 2064 184 2007
Fragments arranged by size:
2439 2064 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4872
Fragments arranged by size:
4872 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3134 3425 4652 5011 5170 518
Size: 648 1968 291 1227 359 159 2042
Fragments arranged by size:
2042 1968 1227 648 359 291 159
MspA1I CmG'CkG
Cuts at: 1606 2602 2641 2920 4110 4787 5935 6180 1606
Size: 996 39 279 1190 677 1148 245 2120
Fragments arranged by size:
2120 1190 1148 996 677 279 245 39
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5203
Fragments arranged by size:
5203 939 552
NdeI CA'TA_TG
Cuts at: 387 2103 2493 387
Size: 1716 390 4588
Fragments arranged by size:
4588 1716 390
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6694
NotI GC'GGCC_GC
Cuts at: 4141 4141
Size: 6694
NspI r_CATG'y
Cuts at: 1132 1229 2758 2932 4052 5597 1132
Size: 97 1529 174 1120 1545 2229
Fragments arranged by size:
2229 1545 1529 1120 174 97
PasI CC'CwG_GG
Cuts at: 1348 3392 1348
Size: 2044 4650
PciI A'CATG_T
Cuts at: 2754 5593 2754
Size: 2839 3855
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6694
PfoI T'CCnGG_A
Cuts at: 3405 3534 3405
Size: 129 6565
PmeI GTTT'AAAC
Cuts at: 3998 3998
Size: 6694
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4763 4795 6276 6308 4763
Size: 32 1481 32 5149
Fragments arranged by size:
5149 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2540 2611 3538 2540
Size: 71 927 5696
Fragments arranged by size:
5696 927 71
PshAI GACnn'nnGTC
Cuts at: 1613 3543 1613
Size: 1930 4764
PsiI TTA'TAA
Cuts at: 4264 4264
Size: 6694
PspOMI G'GGCC_C
Cuts at: 2009 2009
Size: 6694
PspXI vC'TCGA_Gb
Cuts at: 2455 3550 2455
Size: 1095 5599
PstI C_TGCA'G
Cuts at: 830 2820 3528 4046 830
Size: 1990 708 518 3478
Fragments arranged by size:
3478 1990 708 518
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6694
PvuII CAG'CTG
Cuts at: 2641 2920 2641
Size: 279 6415
SacI G_AGCT'C
Cuts at: 3510 3510
Size: 6694
SacII CC_GC'GG
Cuts at: 2603 2603
Size: 6694
SalI G'TCGA_C
Cuts at: 1936 2497 4036 1936
Size: 561 1539 4594
Fragments arranged by size:
4594 1539 561
SapI GCTCTTCn'nnn_
Cuts at: 3194 3249 3194
Size: 55 6639
SbfI CC_TGCA'GG
Cuts at: 4046 4046
Size: 6694
ScaI AGT'ACT
Cuts at: 3809 3886 4942 6654 3809
Size: 77 1056 1712 3849
Fragments arranged by size:
3849 1712 1056 77
SexAI A'CCwGG_T
Cuts at: 2428 2428
Size: 6694
SfcI C'TryA_G
Cuts at: 826 1045 2722 2816 3487 3524 4042 5177 5858
Size: 219 1677 94 671 37 518 1135 681
Cuts at: 5858 6049 826
Size: 191 1471
Fragments arranged by size:
1677 1471 1135 681 671 518 219 191
94 37
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6694
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6694
SmaI CCC'GGG
Cuts at: 1872 4021 1872
Size: 2149 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2455 2909 3550 4798 5699 5961
Size: 197 926 512 454 641 1248 901 262
Cuts at: 5961 820
Size: 1553
Fragments arranged by size:
1553 1248 926 901 641 512 454 262
197
SnaBI TAC'GTA
Cuts at: 493 4485 493
Size: 3992 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6694
SphI G_CATG'C
Cuts at: 1132 2932 4052 1132
Size: 1800 1120 3774
Fragments arranged by size:
3774 1800 1120
SspI AAT'ATT
Cuts at: 5 52 3077 4618 5
Size: 47 3025 1541 2081
Fragments arranged by size:
3025 2081 1541 47
StuI AGG'CCT
Cuts at: 1517 2418 1517
Size: 901 5793
StyI C'CwwG_G
Cuts at: 513 1065 2004 4133 513
Size: 552 939 2129 3074
Fragments arranged by size:
3074 2129 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3488 4721 4738 4891 5076 5521 325
Size: 1255 1908 1233 17 153 185 445 1498
Fragments arranged by size:
1908 1498 1255 1233 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3807 3884 4940
Size: 274 80 33 51 1443 1830 77 1056
Cuts at: 4940 6652 96
Size: 1712 138
Fragments arranged by size:
1830 1712 1443 1056 274 138 80 77
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2111 2143 2230 2262 3152 3184 6654 6686 2111
Size: 32 87 32 890 32 3470 32 2119
Fragments arranged by size:
3470 2119 890 87 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6694
XbaI T'CTAG_A
Cuts at: 4030 4030
Size: 6694
XhoI C'TCGA_G
Cuts at: 1943 2455 3550 1943
Size: 512 1095 5087
Fragments arranged by size:
5087 1095 512
XmaI C'CCGG_G
Cuts at: 1870 4019 1870
Size: 2149 4545
XmnI GAAnn'nnTTC
Cuts at: 4823 4823
Size: 6694
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4502 276
Size: 53 83 186 936 2968 2468
Fragments arranged by size:
2968 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstZ17I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PsrI RsrII SanDI SfiI SfoI SrfI SwaI XcmI