Restriction map for
pFN21AA1469
(Circular) (Six-base) MAPSORT of: pFN21AA1469.seq Check: 4062 from: 1 to: 6652
pFN21AA1469 6652 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4462 278
Size: 53 83 186 936 2926 2468
Fragments arranged by size:
2926 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3995 1937
Size: 2058 4594
Acc65I G'GTAC_C
Cuts at: 3973 3973
Size: 6652
AclI AA'CG_TT
Cuts at: 1563 4779 5152 1563
Size: 3216 373 3063
Fragments arranged by size:
3216 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2183 2217 4715 6099 6367 1632
Size: 551 34 2498 1384 268 1917
Fragments arranged by size:
2498 1917 1384 551 268 34
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6455
AflIII A'CryG_T
Cuts at: 1203 5551 6528 1203
Size: 4348 977 1327
Fragments arranged by size:
4348 1327 977
AgeI A'CCGG_T
Cuts at: 5483 5483
Size: 6652
AhdI GACnn_n'nnGTC
Cuts at: 5381 5381
Size: 6652
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6652
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6652
AlwNI CAG_nnn'CTG
Cuts at: 3737 4395 5967 3737
Size: 658 1572 4422
Fragments arranged by size:
4422 1572 658
ApaLI G'TGCA_C
Cuts at: 4709 5865 4709
Size: 1156 5496
ApoI r'AATT_y
Cuts at: 1493 2294 2382 2707 3961 4187 4339 6448 1493
Size: 801 88 325 1254 226 152 2109 1697
Fragments arranged by size:
2109 1697 1254 801 325 226 152 88
AseI AT'TA_AT
Cuts at: 160 1032 5206 160
Size: 872 4174 1606
Fragments arranged by size:
4174 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6652
AvaI C'yCGr_G
Cuts at: 1870 1943 3977 1870
Size: 73 2034 4545
Fragments arranged by size:
4545 2034 73
BamHI G'GATC_C
Cuts at: 3982 4349 3982
Size: 367 6285
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2788 3973 5428 618
Size: 325 217 627 1001 1185 1455 1842
Fragments arranged by size:
1842 1455 1185 1001 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3000 3971 1391
Size: 1609 971 4072
Fragments arranged by size:
4072 1609 971
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5681
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2807 2841 4843 4877 1903
Size: 34 870 34 2002 34 3678
Fragments arranged by size:
3678 2002 870 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3138 4544 5754 3138
Size: 1406 1210 4036
Fragments arranged by size:
4036 1406 1210
BclI T'GATC_A
Cuts at: 1548 2007 1548
Size: 459 6193
BfrBI ATG'CAT
Cuts at: 3438 3438
Size: 6652
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5263 136
Size: 107 122 71 631 605 3591 1525
Fragments arranged by size:
3591 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6647 6647
Size: 6652
BlpI GC'TnA_GC
Cuts at: 4069 4069
Size: 6652
Bme1580I G_kGCm'C
Cuts at: 1790 2793 4713 5869 1790
Size: 1003 1920 1156 2573
Fragments arranged by size:
2573 1920 1156 1003
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6652
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3341 4383 5341
Size: 416 53 83 399 24 1924 1042 958
Cuts at: 5341 442
Size: 1753
Fragments arranged by size:
1924 1753 1042 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6652
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2041 3138 3431 5312 1239
Size: 802 1097 293 1881 2579
Fragments arranged by size:
2579 1881 1097 802 293
Bpu10I CC'TnA_GC
Cuts at: 2501 2501
Size: 6652
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3208 3667 4777 5642 5940 3208
Size: 459 1110 865 298 3920
Fragments arranged by size:
3920 1110 865 459 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2600 5315 882
Size: 617 1101 2715 2219
Fragments arranged by size:
2715 2219 1101 617
BsaAI yAC'GTr
Cuts at: 493 1204 2954 4443 493
Size: 711 1750 1489 2702
Fragments arranged by size:
2702 1750 1489 711
BsaBI GATnn'nnATC
Cuts at: 2556 6646 2556
Size: 4090 2562
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4459 4841 5476 275
Size: 53 83 186 936 2926 382 635 1451
Fragments arranged by size:
2926 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5085 5483 5757 5904 6315 5085
Size: 398 274 147 411 5422
Fragments arranged by size:
5422 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6427
Fragments arranged by size:
6427 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3724 3839 1186
Size: 2538 115 3999
Fragments arranged by size:
3999 2538 115
BseYI C'CCAG_C
Cuts at: 2222 5855 6351 2222
Size: 3633 496 2523
Fragments arranged by size:
3633 2523 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2706 3492 1163
Size: 573 970 786 4323
Fragments arranged by size:
4323 970 786 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4102 4863 5891 1540
Size: 275 186 2101 761 1028 2301
Fragments arranged by size:
2301 2101 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4713 4798 5869 4713
Size: 85 1071 5496
Fragments arranged by size:
5496 1071 85
BsmI GAATG_Cn'
Cuts at: 2450 4163 4256 2450
Size: 1713 93 4846
Fragments arranged by size:
4846 1713 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2793 3000 3971 4713 4798 5869 1391
Size: 399 1003 207 971 742 85 1071 2174
Fragments arranged by size:
2174 1071 1003 971 742 399 207 85
BspEI T'CCGG_A
Cuts at: 6315 6315
Size: 6652
BspHI T'CATG_A
Cuts at: 4541 6271 4541
Size: 1730 4922
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4007 844
Size: 3163 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4539 1394
Size: 429 2716 3507
Fragments arranged by size:
3507 2716 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3627 3811 5147 5321 65
Size: 1183 2379 184 1336 174 1396
Fragments arranged by size:
2379 1396 1336 1183 184 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5296 5483 1700
Size: 110 3486 187 2869
Fragments arranged by size:
3486 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3522 3870 96
Size: 3426 348 2878
Fragments arranged by size:
3426 2878 348
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6652
BssSI C'ACGA_G
Cuts at: 4712 5724 4712
Size: 1012 5640
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6652
BstBI TT'CG_AA
Cuts at: 3517 5456 3517
Size: 1939 4713
BstEII G'GTnAC_C
Cuts at: 1184 3622 1184
Size: 2438 4214
BstYI r'GATC_y
Cuts at: 1972 3043 3353 3982 4349 4734 4751 6203 6289
Size: 1071 310 629 367 385 17 1452 86
Cuts at: 6289 6301 6647 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1071 629 385 367 346 310
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2750 2865 2750
Size: 115 6537
BtgI C'CryG_G
Cuts at: 513 1065 3447 513
Size: 552 2382 3718
Fragments arranged by size:
3718 2382 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2130 487
Size: 683 56 904 5009
Fragments arranged by size:
5009 904 683 56
BtsI GCAGTG_nn'
Cuts at: 3105 3394 4168 4962 4982 3105
Size: 289 774 794 20 4775
Fragments arranged by size:
4775 794 774 289 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6652
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2974 3009 3125 3160 529
Size: 35 2410 35 116 35 4021
Fragments arranged by size:
4021 2410 116 35 35 35
DraI TTT'AAA
Cuts at: 3956 3956
Size: 6652
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2420 2692 5659 809
Size: 507 1104 272 2967 1802
Fragments arranged by size:
2967 1802 1104 507 272
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3146 4099 4988
Size: 54 1425 50 275 18 1316 953 889
Cuts at: 4988 6617 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1316 953 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4099 1537
Size: 275 2287 4090
Fragments arranged by size:
4090 2287 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2436 2592 3683 4582 1353
Size: 1083 156 1091 899 3423
Fragments arranged by size:
3423 1091 1083 899 156
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5239 5613 5759 387
Size: 1064 138 3650 374 146 1280
Fragments arranged by size:
3650 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2041 2183 2217 3138 3431 4715 5312
Size: 393 409 142 34 921 293 1284 597
Cuts at: 5312 6099 6367 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1284 921 787 597 409 393 293
268 142 34
EcoNI CCTnn'n_nnAGG
Cuts at: 3135 3135
Size: 6652
EcoRI G'AATT_C
Cuts at: 2707 3961 4339 2707
Size: 1254 378 5020
Fragments arranged by size:
5020 1254 378
FspI TGC'GCA
Cuts at: 5158 5158
Size: 6652
HaeII r_GCGC'y
Cuts at: 1678 5799 1678
Size: 4121 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2402 2434 5293 5325 5367
Size: 32 135 32 830 32 2859 32 42
Cuts at: 5367 5399 1373
Size: 32 2626
Fragments arranged by size:
2859 2626 830 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2986 3996 4242 669
Size: 988 281 1048 1010 246 3079
Fragments arranged by size:
3079 1048 1010 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2756 748
Size: 2008 4644
HpaI GTT'AAC
Cuts at: 4242 4242
Size: 6652
KpnI G_GTAC'C
Cuts at: 3977 3977
Size: 6652
MfeI C'AATT_G
Cuts at: 3369 4251 3369
Size: 882 5770
MluI A'CGCG_T
Cuts at: 6528 6528
Size: 6652
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2186 2497 5766 5950 1305
Size: 881 311 3269 184 2007
Fragments arranged by size:
3269 2007 881 311 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3148 10
Size: 54 1425 343 1316 3514
Fragments arranged by size:
3514 1425 1316 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2104 2409 3666 4610 4969 5128 518
Size: 648 938 305 1257 944 359 159 2042
Fragments arranged by size:
2042 1257 944 938 648 359 305 159
MspA1I CmG'CkG
Cuts at: 1606 3321 4068 4745 5893 6138 1606
Size: 1715 747 677 1148 245 2120
Fragments arranged by size:
2120 1715 1148 747 677 245
NcoI C'CATG_G
Cuts at: 513 1065 3447 513
Size: 552 2382 3718
Fragments arranged by size:
3718 2382 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6652
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6652
NotI GC'GGCC_GC
Cuts at: 4099 4099
Size: 6652
NsiI A_TGCA'T
Cuts at: 3440 3440
Size: 6652
NspI r_CATG'y
Cuts at: 1132 1229 4010 5555 1132
Size: 97 2781 1545 2229
Fragments arranged by size:
2781 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2514 1348
Size: 1166 5486
PciI A'CATG_T
Cuts at: 5551 5551
Size: 6652
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6652
PfoI T'CCnGG_A
Cuts at: 3762 3762
Size: 6652
PmeI GTTT'AAAC
Cuts at: 3956 3956
Size: 6652
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4721 4753 6234 6266 4721
Size: 32 1481 32 5107
Fragments arranged by size:
5107 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6652
PsiI TTA'TAA
Cuts at: 4222 4222
Size: 6652
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3426 3458 3426
Size: 32 6620
PstI C_TGCA'G
Cuts at: 830 2431 4004 830
Size: 1601 1573 3478
Fragments arranged by size:
3478 1601 1573
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6652
PvuII CAG'CTG
Cuts at: 3321 3321
Size: 6652
SalI G'TCGA_C
Cuts at: 1936 3994 1936
Size: 2058 4594
SapI GCTCTTCn'nnn_
Cuts at: 3683 3683
Size: 6652
SbfI CC_TGCA'GG
Cuts at: 4004 4004
Size: 6652
ScaI AGT'ACT
Cuts at: 3395 4900 6612 3395
Size: 1505 1712 3435
Fragments arranged by size:
3435 1712 1505
SfcI C'TryA_G
Cuts at: 826 1045 2427 2532 3910 4000 5135 5816 6007
Size: 219 1382 105 1378 90 1135 681 191
Cuts at: 6007 826
Size: 1471
Fragments arranged by size:
1471 1382 1378 1135 681 219 191 105
90
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6652
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6652
SmaI CCC'GGG
Cuts at: 1872 3979 1872
Size: 2107 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3223 3682 4756 5657 5919 820
Size: 197 926 1280 459 1074 901 262 1553
Fragments arranged by size:
1553 1280 1074 926 901 459 262 197
SnaBI TAC'GTA
Cuts at: 493 4443 493
Size: 3950 2702
SpeI A'CTAG_T
Cuts at: 152 2600 152
Size: 2448 4204
SphI G_CATG'C
Cuts at: 1132 4010 1132
Size: 2878 3774
SspI AAT'ATT
Cuts at: 5 52 4576 5
Size: 47 4524 2081
Fragments arranged by size:
4524 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6652
StyI C'CwwG_G
Cuts at: 513 1065 3136 3447 4091 513
Size: 552 2071 311 644 3074
Fragments arranged by size:
3074 2071 644 552 311
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4679 4696 4849 5034 5479 325
Size: 1255 3099 17 153 185 445 1498
Fragments arranged by size:
3099 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3393 3522 3870
Size: 274 80 33 51 1443 1416 129 348
Cuts at: 3870 4898 6610 96
Size: 1028 1712 138
Fragments arranged by size:
1712 1443 1416 1028 348 274 138 129
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3112 3144 3202 3234 3832 3864 6612 6644 3112
Size: 32 58 32 598 32 2748 32 3120
Fragments arranged by size:
3120 2748 598 58 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6652
XbaI T'CTAG_A
Cuts at: 2620 3988 2620
Size: 1368 5284
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3586 3586
Size: 6652
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6652
XmaI C'CCGG_G
Cuts at: 1870 3977 1870
Size: 2107 4545
XmnI GAAnn'nnTTC
Cuts at: 3021 4781 3021
Size: 1760 4892
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4460 276
Size: 53 83 186 936 2926 2468
Fragments arranged by size:
2926 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI