Restriction map for
pFN21AA1464
(Circular) (Six-base) MAPSORT of: pFN21AA1464.seq Check: 6259 from: 1 to: 6565
pFN21AA1464 6565 bp
With 182 enzymes: SgfI *
February 27, 2009 14:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2549 2549
Size: 6565
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4375 278
Size: 53 83 186 936 2839 2468
Fragments arranged by size:
2839 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3908 1937
Size: 1971 4594
Acc65I G'GTAC_C
Cuts at: 3886 3886
Size: 6565
AclI AA'CG_TT
Cuts at: 1563 4692 5065 1563
Size: 3129 373 3063
Fragments arranged by size:
3129 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3616 4628 6012 6280 1632
Size: 1984 1012 1384 268 1917
Fragments arranged by size:
1984 1917 1384 1012 268
AfeI AGC'GCT
Cuts at: 2951 2951
Size: 6565
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6368
AflIII A'CryG_T
Cuts at: 1203 5464 6441 1203
Size: 4261 977 1327
Fragments arranged by size:
4261 1327 977
AgeI A'CCGG_T
Cuts at: 5396 5396
Size: 6565
AhdI GACnn_n'nnGTC
Cuts at: 5294 5294
Size: 6565
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6565
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6565
AlwNI CAG_nnn'CTG
Cuts at: 2107 2128 2905 3748 4308 5880 2107
Size: 21 777 843 560 1572 2792
Fragments arranged by size:
2792 1572 843 777 560 21
ApaLI G'TGCA_C
Cuts at: 4622 5778 4622
Size: 1156 5409
ApoI r'AATT_y
Cuts at: 1493 3598 3874 4100 4252 6361 1493
Size: 2105 276 226 152 2109 1697
Fragments arranged by size:
2109 2105 1697 276 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5119 160
Size: 872 4087 1606
Fragments arranged by size:
4087 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6565
AvaI C'yCGr_G
Cuts at: 1870 1943 2026 2461 3813 3890 1870
Size: 73 83 435 1352 77 4545
Fragments arranged by size:
4545 1352 435 83 77 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3319 3352 3319
Size: 33 6532
BamHI G'GATC_C
Cuts at: 3895 4262 3895
Size: 367 6198
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2537 3434 3886 5341 618
Size: 325 217 627 750 897 452 1455 1842
Fragments arranged by size:
1842 1455 897 750 627 452 325 217
BanII G_rGCy'C
Cuts at: 1391 2180 3062 3884 1391
Size: 789 882 822 4072
Fragments arranged by size:
4072 882 822 789
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5594
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3163 3197 4756 4790 1903
Size: 34 1226 34 1559 34 3678
Fragments arranged by size:
3678 1559 1226 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2142 4457 5667 2142
Size: 2315 1210 3040
Fragments arranged by size:
3040 2315 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6565
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2925 3672
Size: 107 122 71 631 605 337 916 747
Cuts at: 3672 5176 136
Size: 1504 1525
Fragments arranged by size:
1525 1504 916 747 631 605 337 122
107 71
BglII A'GATC_T
Cuts at: 6560 6560
Size: 6565
BlpI GC'TnA_GC
Cuts at: 2108 3982 2108
Size: 1874 4691
Bme1580I G_kGCm'C
Cuts at: 1790 2723 2749 4626 5782 1790
Size: 933 26 1877 1156 2573
Fragments arranged by size:
2573 1877 1156 933 26
BmgBI CAC'GTC
Cuts at: 1318 2506 1318
Size: 1188 5377
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3120 3662 4296
Size: 416 53 83 399 24 1703 542 634
Cuts at: 4296 5254 442
Size: 958 1753
Fragments arranged by size:
1753 1703 958 634 542 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6565
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3316 3316
Size: 6565
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2194 2933 2990 3679 5225 1239
Size: 955 739 57 689 1546 2579
Fragments arranged by size:
2579 1546 955 739 689 57
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2361 2984 4690 5555 5853 2361
Size: 623 1706 865 298 3073
Fragments arranged by size:
3073 1706 865 623 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2210 2933 3344 5228 882
Size: 617 711 723 411 1884 2219
Fragments arranged by size:
2219 1884 723 711 617 411
BsaAI yAC'GTr
Cuts at: 493 1204 4356 493
Size: 711 3152 2702
Fragments arranged by size:
3152 2702 711
BsaBI GATnn'nnATC
Cuts at: 6559 6559
Size: 6565
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2382 2646 2873 3180
Size: 53 83 186 936 849 264 227 307
Cuts at: 3180 4372 4754 5389 275
Size: 1192 382 635 1451
Fragments arranged by size:
1451 1192 936 849 635 382 307 264
227 186 83 53
BsaWI w'CCGG_w
Cuts at: 4998 5396 5670 5817 6228 4998
Size: 398 274 147 411 5335
Fragments arranged by size:
5335 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3080 3110 1178
Size: 30 165 30 1677 30 4633
Fragments arranged by size:
4633 1677 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2047 2449 2978 3223 3238 3241 3244 3247
Size: 861 402 529 245 15 3 3 3
Cuts at: 3247 1186
Size: 4504
Fragments arranged by size:
4504 861 529 402 245 15 3 3
3
BseYI C'CCAG_C
Cuts at: 2033 2072 2946 3101 3164 3227 3321 5768 6264
Size: 39 874 155 63 63 94 2447 496
Cuts at: 6264 2033
Size: 2334
Fragments arranged by size:
2447 2334 874 496 155 94 63 63
39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3207 1163
Size: 573 1471 4521
Fragments arranged by size:
4521 1471 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4015 4776 5804 1540
Size: 275 186 2014 761 1028 2301
Fragments arranged by size:
2301 2014 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2976 3062 3387 4626 4711 5782 2976
Size: 86 325 1239 85 1071 3759
Fragments arranged by size:
3759 1239 1071 325 86 85
BsmI GAATG_Cn'
Cuts at: 2446 4076 4169 2446
Size: 1630 93 4842
Fragments arranged by size:
4842 1630 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2496 2496
Size: 6565
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2180 2723 2749 2976 3062 3387 3884
Size: 399 390 543 26 227 86 325 497
Cuts at: 3884 4626 4711 5782 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 543 497 399 390 325
227 86 85 26
BspEI T'CCGG_A
Cuts at: 6228 6228
Size: 6565
BspHI T'CATG_A
Cuts at: 4454 6184 4454
Size: 1730 4835
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2302 2549 3462 3756 3920 844
Size: 1458 247 913 294 164 3489
Fragments arranged by size:
3489 1458 913 294 247 164
BsrBI CCG'CTC
Cuts at: 1394 1823 4452 1394
Size: 429 2629 3507
Fragments arranged by size:
3507 2629 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3181 5060 5234 65
Size: 1183 1933 1879 174 1396
Fragments arranged by size:
1933 1879 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2112 3006 5209 5396 1700
Size: 110 302 894 2203 187 2869
Fragments arranged by size:
2869 2203 894 302 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6565
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6565
BssSI C'ACGA_G
Cuts at: 2798 4625 5637 2798
Size: 1827 1012 3726
Fragments arranged by size:
3726 1827 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2770 794
Size: 1976 4589
BstBI TT'CG_AA
Cuts at: 5369 5369
Size: 6565
BstEII G'GTnAC_C
Cuts at: 1184 2426 1184
Size: 1242 5323
BstXI CCAn_nnnn'nTGG
Cuts at: 2480 2570 2480
Size: 90 6475
BstYI r'GATC_y
Cuts at: 1972 2895 3895 4262 4647 4664 6116 6202 6214
Size: 923 1000 367 385 17 1452 86 12
Cuts at: 6214 6560 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1000 923 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2136 2431 2826 513
Size: 552 939 132 295 395 4252
Fragments arranged by size:
4252 939 552 395 295 132
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5826
Fragments arranged by size:
5826 683 56
BtsI GCAGTG_nn'
Cuts at: 2728 2775 4081 4875 4895 2728
Size: 47 1306 794 20 4398
Fragments arranged by size:
4398 1306 794 47 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6565
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2815 2850 3406 3441 529
Size: 35 2251 35 556 35 3653
Fragments arranged by size:
3653 2251 556 35 35 35
DraI TTT'AAA
Cuts at: 3869 3869
Size: 6565
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3044 3466 5572 809
Size: 507 1728 422 2106 1802
Fragments arranged by size:
2106 1802 1728 507 422
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2470 2560 2595
Size: 54 1425 50 275 18 640 90 35
Cuts at: 2595 3547 3673 4012 4901 6530 8
Size: 952 126 339 889 1629 43
Fragments arranged by size:
1629 1425 952 889 640 339 275 126
90 54 50 43 35 18
EagI C'GGCC_G
Cuts at: 1537 1812 4012 1537
Size: 275 2200 4090
Fragments arranged by size:
4090 2200 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2338 2996 3271 4495 1353
Size: 985 658 275 1224 3423
Fragments arranged by size:
3423 1224 985 658 275
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5152 5526 5672 387
Size: 1064 138 3563 374 146 1280
Fragments arranged by size:
3563 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3060 3060
Size: 6565
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2194 2933 2990 3616 3679 4628 5225
Size: 393 562 739 57 626 63 949 597
Cuts at: 5225 6012 6280 1239
Size: 787 268 1524
Fragments arranged by size:
1524 949 787 739 626 597 562 393
268 63 57
EcoNI CCTnn'n_nnAGG
Cuts at: 3782 3782
Size: 6565
EcoO109I rG'GnC_Cy
Cuts at: 3451 3451
Size: 6565
EcoRI G'AATT_C
Cuts at: 3874 4252 3874
Size: 378 6187
FspI TGC'GCA
Cuts at: 5071 5071
Size: 6565
HaeII r_GCGC'y
Cuts at: 1678 2073 2119 2128 2953 3729 5712 1678
Size: 395 46 9 825 776 1983 2531
Fragments arranged by size:
2531 1983 825 776 395 46 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2252 2284 2493 2525 2880
Size: 32 135 32 680 32 209 32 355
Cuts at: 2880 2912 5206 5238 5280 5312 1373
Size: 32 2294 32 42 32 2626
Fragments arranged by size:
2626 2294 680 355 209 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2143 2386 2524 3909 4155 669
Size: 988 281 205 243 138 1385 246 3079
Fragments arranged by size:
3079 1385 988 281 246 243 205 138
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6565
HpaI GTT'AAC
Cuts at: 4155 4155
Size: 6565
KpnI G_GTAC'C
Cuts at: 3890 3890
Size: 6565
MfeI C'AATT_G
Cuts at: 4164 4164
Size: 6565
MluI A'CGCG_T
Cuts at: 6441 6441
Size: 6565
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3321 5679 5863 1305
Size: 2016 2358 184 2007
Fragments arranged by size:
2358 2016 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2472 2562 3675 10
Size: 54 1425 343 640 90 1113 2900
Fragments arranged by size:
2900 1425 1113 640 343 90 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2259 2421 2436 3614 3793 4523 4882
Size: 648 1093 162 15 1178 179 730 359
Cuts at: 4882 5041 518
Size: 159 2042
Fragments arranged by size:
2042 1178 1093 730 648 359 179 162
159 15
MspA1I CmG'CkG
Cuts at: 1606 2138 2161 3168 3981 4658 5806 6051 1606
Size: 532 23 1007 813 677 1148 245 2120
Fragments arranged by size:
2120 1148 1007 813 677 532 245 23
NaeI GCC'GGC
Cuts at: 2114 3008 2114
Size: 894 5671
NcoI C'CATG_G
Cuts at: 513 1065 2004 2431 513
Size: 552 939 427 4647
Fragments arranged by size:
4647 939 552 427
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6565
NgoMIV G'CCGG_C
Cuts at: 2112 3006 2112
Size: 894 5671
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6565
NotI GC'GGCC_GC
Cuts at: 4012 4012
Size: 6565
NspI r_CATG'y
Cuts at: 1132 1229 2695 3142 3415 3923 5468 1132
Size: 97 1466 447 273 508 1545 2229
Fragments arranged by size:
2229 1545 1466 508 447 273 97
PasI CC'CwG_GG
Cuts at: 1348 2093 2212 2960 3778 1348
Size: 745 119 748 818 4135
Fragments arranged by size:
4135 818 748 745 119
PciI A'CATG_T
Cuts at: 5464 5464
Size: 6565
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2905 1108
Size: 1797 4768
PfoI T'CCnGG_A
Cuts at: 2171 2171
Size: 6565
PmeI GTTT'AAAC
Cuts at: 3869 3869
Size: 6565
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4634 4666 6147 6179 4634
Size: 32 1481 32 5020
Fragments arranged by size:
5020 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3451 3451
Size: 6565
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6565
PsiI TTA'TAA
Cuts at: 4135 4135
Size: 6565
PstI C_TGCA'G
Cuts at: 830 2625 2778 3459 3624 3917 830
Size: 1795 153 681 165 293 3478
Fragments arranged by size:
3478 1795 681 293 165 153
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6565
PvuII CAG'CTG
Cuts at: 3168 3168
Size: 6565
SacI G_AGCT'C
Cuts at: 3062 3062
Size: 6565
SacII CC_GC'GG
Cuts at: 2139 2139
Size: 6565
SalI G'TCGA_C
Cuts at: 1936 3907 1936
Size: 1971 4594
SbfI CC_TGCA'GG
Cuts at: 3917 3917
Size: 6565
ScaI AGT'ACT
Cuts at: 4813 6525 4813
Size: 1712 4853
SfcI C'TryA_G
Cuts at: 826 1045 2621 2774 3169 3455 3620 3913 5048
Size: 219 1576 153 395 286 165 293 1135
Cuts at: 5048 5729 5920 826
Size: 681 191 1471
Fragments arranged by size:
1576 1471 1135 681 395 293 286 219
191 165 153
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6565
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6565
SmaI CCC'GGG
Cuts at: 1872 2028 3892 1872
Size: 156 1864 4545
Fragments arranged by size:
4545 1864 156
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2376 2999 4669 5570 5832 820
Size: 197 926 433 623 1670 901 262 1553
Fragments arranged by size:
1670 1553 926 901 623 433 262 197
SnaBI TAC'GTA
Cuts at: 493 4356 493
Size: 3863 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6565
SphI G_CATG'C
Cuts at: 1132 3923 1132
Size: 2791 3774
SspI AAT'ATT
Cuts at: 5 52 4489 5
Size: 47 4437 2081
Fragments arranged by size:
4437 2081 47
StuI AGG'CCT
Cuts at: 1517 2619 3822 1517
Size: 1102 1203 4260
Fragments arranged by size:
4260 1203 1102
StyI C'CwwG_G
Cuts at: 513 1065 2004 2181 2431 2569 2709 4004 513
Size: 552 939 177 250 138 140 1295 3074
Fragments arranged by size:
3074 1295 939 552 250 177 140 138
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2297 2960 3241 4592 4609 4762 4947
Size: 1255 717 663 281 1351 17 153 185
Cuts at: 4947 5392 325
Size: 445 1498
Fragments arranged by size:
1498 1351 1255 717 663 445 281 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4811 6523 96
Size: 274 80 33 51 1443 2834 1712 138
Fragments arranged by size:
2834 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3025 3057 3692 3724 6525 6557 3025
Size: 32 635 32 2801 32 3033
Fragments arranged by size:
3033 2801 635 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6565
XbaI T'CTAG_A
Cuts at: 3901 3901
Size: 6565
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2438 2438
Size: 6565
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6565
XmaI C'CCGG_G
Cuts at: 1870 2026 3890 1870
Size: 156 1864 4545
Fragments arranged by size:
4545 1864 156
XmnI GAAnn'nnTTC
Cuts at: 4694 4694
Size: 6565
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4373 276
Size: 53 83 186 936 2839 2468
Fragments arranged by size:
2839 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SacII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BbeI BbvCI BfrBI Bpu10I
BsiWI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI
KasI NarI NruI NsiI PacI PmlI PspOMI PspXI
PsrI RsrII SanDI SapI SexAI SfiI SfoI SrfI
SwaI