Restriction map for
pFN21AA1462
(Circular) (Six-base) MAPSORT of: pFN21AA1462.seq Check: 8254 from: 1 to: 8782
pFN21AA1462 8782 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3665 3665
Size: 8782
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2183 6592 278
Size: 53 83 186 936 647 4409 2468
Fragments arranged by size:
4409 2468 936 647 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6125 1937
Size: 4188 4594
Acc65I G'GTAC_C
Cuts at: 6103 6103
Size: 8782
AclI AA'CG_TT
Cuts at: 1563 6909 7282 1563
Size: 5346 373 3063
Fragments arranged by size:
5346 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3150 5623 5815 6845 8229 8497 1632
Size: 1518 2473 192 1030 1384 268 1917
Fragments arranged by size:
2473 1917 1518 1384 1030 268 192
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8585
AflIII A'CryG_T
Cuts at: 1203 2197 2659 7681 8658 1203
Size: 994 462 5022 977 1327
Fragments arranged by size:
5022 1327 994 977 462
AgeI A'CCGG_T
Cuts at: 4056 4217 4471 7613 4056
Size: 161 254 3142 5225
Fragments arranged by size:
5225 3142 254 161
AhdI GACnn_n'nnGTC
Cuts at: 5149 7511 5149
Size: 2362 6420
AleI CACnn'nnGTG
Cuts at: 1166 4779 1166
Size: 3613 5169
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4855 5374 1184
Size: 3671 519 4592
Fragments arranged by size:
4592 3671 519
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5585 5617 5585
Size: 32 8750
AlwNI CAG_nnn'CTG
Cuts at: 2131 2537 3002 3874 4178 5564 5690 5771 6525
Size: 406 465 872 304 1386 126 81 754
Cuts at: 6525 8097 2131
Size: 1572 2816
Fragments arranged by size:
2816 1572 1386 872 754 465 406 304
126 81
ApaI G_GGCC'C
Cuts at: 4430 4430
Size: 8782
ApaLI G'TGCA_C
Cuts at: 4830 6839 7995 4830
Size: 2009 1156 5617
Fragments arranged by size:
5617 2009 1156
ApoI r'AATT_y
Cuts at: 1493 2668 2739 2806 2824 6091 6317 6469 8578
Size: 1175 71 67 18 3267 226 152 2109
Cuts at: 8578 1493
Size: 1697
Fragments arranged by size:
3267 2109 1697 1175 226 152 71 67
18
AseI AT'TA_AT
Cuts at: 160 1032 7336 160
Size: 872 6304 1606
Fragments arranged by size:
6304 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8782
AvaI C'yCGr_G
Cuts at: 1870 1943 2717 3518 4798 5899 6107 1870
Size: 73 774 801 1280 1101 208 4545
Fragments arranged by size:
4545 1280 1101 801 774 208 73
AvrII C'CTAG_G
Cuts at: 4991 5756 4991
Size: 765 8017
BamHI G'GATC_C
Cuts at: 5268 6112 6479 5268
Size: 844 367 7571
Fragments arranged by size:
7571 844 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2091 3088 3419 4053 4324
Size: 325 217 627 304 997 331 634 271
Cuts at: 4324 4696 5183 6042 6103 7558 618
Size: 372 487 859 61 1455 1842
Fragments arranged by size:
1842 1455 997 859 634 627 487 372
331 325 304 271 217 61
BanII G_rGCy'C
Cuts at: 1391 2250 2988 3206 3225 3854 4101 4384 4422
Size: 859 738 218 19 629 247 283 38
Cuts at: 4422 4430 4530 4709 5001 5100 5133 5163 5346
Size: 8 100 179 292 99 33 30 183
Cuts at: 5346 5474 5512 5583 5804 5898 6101 1391
Size: 128 38 71 221 94 203 4072
Fragments arranged by size:
4072 859 738 629 292 283 247 221
218 203 183 179 128 100 99 94
71 38 38 33 30 19 8
BbeI G_GCGC'C
Cuts at: 2095 2095
Size: 8782
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2025 3087 3342 3625 3879 3981 4932
Size: 971 126 1062 255 283 254 102 951
Cuts at: 4932 928
Size: 4778
Fragments arranged by size:
4778 1062 971 951 283 255 254 126
102
BbvCI CC'TCA_GC
Cuts at: 2305 4271 2305
Size: 1966 6816
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5336 5370 6973 7007 1903
Size: 34 3399 34 1603 34 3678
Fragments arranged by size:
3678 3399 1603 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2033 2796 6674 7884 2033
Size: 763 3878 1210 2931
Fragments arranged by size:
3878 2931 1210 763
BclI T'GATC_A
Cuts at: 1548 3826 4222 4497 1548
Size: 2278 396 275 5833
Fragments arranged by size:
5833 2278 396 275
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3220 5507 7393
Size: 107 122 71 631 605 1548 2287 1886
Cuts at: 7393 136
Size: 1525
Fragments arranged by size:
2287 1886 1548 1525 631 605 122 107
71
BglII A'GATC_T
Cuts at: 3955 8777 3955
Size: 4822 3960
BlpI GC'TnA_GC
Cuts at: 2845 3315 3878 4843 5340 6199 2845
Size: 470 563 965 497 859 5428
Fragments arranged by size:
5428 965 859 563 497 470
Bme1580I G_kGCm'C
Cuts at: 1790 3093 3612 4056 4246 4430 4834 5938 6047
Size: 1303 519 444 190 184 404 1104 109
Cuts at: 6047 6843 7999 1790
Size: 796 1156 2573
Fragments arranged by size:
2573 1303 1156 1104 796 519 444 404
190 184 109
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8782
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2118 3192 3702
Size: 416 53 83 399 24 701 1074 510
Cuts at: 3702 3755 3813 6513 7471 442
Size: 53 58 2700 958 1753
Fragments arranged by size:
2700 1753 1074 958 701 510 416 399
83 58 53 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8782
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3873 5818 3873
Size: 1945 6837
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2426 3000 3352 3920 4514 4771 4901 5124
Size: 1187 574 352 568 594 257 130 223
Cuts at: 5124 5965 7442 1239
Size: 841 1477 2579
Fragments arranged by size:
2579 1477 1187 841 594 574 568 352
257 223 130
Bpu10I CC'TnA_GC
Cuts at: 2305 4271 4395 5571 5577 5825 2305
Size: 1966 124 1176 6 248 5262
Fragments arranged by size:
5262 1966 1176 248 124 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2867 3369 4013 4282 6907 7772 8070 2867
Size: 502 644 269 2625 865 298 3579
Fragments arranged by size:
3579 2625 865 644 502 298 269
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2049 3549 7445 882
Size: 617 550 1500 3896 2219
Fragments arranged by size:
3896 2219 1500 617 550
BsaAI yAC'GTr
Cuts at: 493 1204 2449 3025 4781 6573 493
Size: 711 1245 576 1756 1792 2702
Fragments arranged by size:
2702 1792 1756 1245 711 576
BsaBI GATnn'nnATC
Cuts at: 8776 8776
Size: 8782
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2092 2180 2964 4331
Size: 53 83 186 936 559 88 784 1367
Cuts at: 4331 5856 6589 6971 7606 275
Size: 1525 733 382 635 1451
Fragments arranged by size:
1525 1451 1367 936 784 733 635 559
382 186 88 83 53
BsaWI w'CCGG_w
Cuts at: 2349 4056 4217 4471 5313 7215 7613 7887 8034
Size: 1707 161 254 842 1902 398 274 147
Cuts at: 8034 8445 2349
Size: 411 2686
Fragments arranged by size:
2686 1902 1707 842 411 398 274 254
161 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2270 2300 2609 2639 2646
Size: 30 165 30 867 30 309 30 7
Cuts at: 2646 2676 5585 5615 5895 5925 5963 5993 1178
Size: 30 2909 30 280 30 38 30 3967
Fragments arranged by size:
3967 2909 867 309 280 165 38 30
30 30 30 30 30 30 30 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2937 4109 4598 4772 5502 1186
Size: 1751 1172 489 174 730 4466
Fragments arranged by size:
4466 1751 1172 730 489 174
BseYI C'CCAG_C
Cuts at: 2062 3143 4132 5398 5582 7985 8481 2062
Size: 1081 989 1266 184 2403 496 2363
Fragments arranged by size:
2403 2363 1266 1081 989 496 184
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4170 4884 5344 1163
Size: 573 2434 714 460 4601
Fragments arranged by size:
4601 2434 714 573 460
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3135 6232 6993 8021 1540
Size: 275 186 1134 3097 761 1028 2301
Fragments arranged by size:
3097 2301 1134 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2258 2397 2988 3656 4530 4621 4834 5529 5804
Size: 139 591 668 874 91 213 695 275
Cuts at: 5804 6843 6928 7999 2258
Size: 1039 85 1071 3041
Fragments arranged by size:
3041 1071 1039 874 695 668 591 275
213 139 91 85
BsmI GAATG_Cn'
Cuts at: 3411 4033 5842 6293 6386 3411
Size: 622 1809 451 93 5807
Fragments arranged by size:
5807 1809 622 451 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2250 2258 2397 2988 3093 3206 3225
Size: 399 460 8 139 591 105 113 19
Cuts at: 3225 3612 3656 3854 4056 4101 4246 4384 4422
Size: 387 44 198 202 45 145 138 38
Cuts at: 4422 4430 4530 4621 4709 4834 5001 5100 5133
Size: 8 100 91 88 125 167 99 33
Cuts at: 5133 5163 5346 5474 5512 5529 5583 5804 5898
Size: 30 183 128 38 17 54 221 94
Cuts at: 5898 5938 6047 6101 6843 6928 7999 1391
Size: 40 109 54 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 591 460 399 387 221
202 198 183 167 145 139 138 128
125 113 109 105 100 99 94 91
88 85 54 54 45 44 40 38
38 33 30 19 17 8 8
BspEI T'CCGG_A
Cuts at: 5313 8445 5313
Size: 3132 5650
BspHI T'CATG_A
Cuts at: 6671 8401 6671
Size: 1730 7052
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3665 4901 6137 844
Size: 2821 1236 1236 3489
Fragments arranged by size:
3489 2821 1236 1236
BsrBI CCG'CTC
Cuts at: 1394 1823 2927 5321 6669 1394
Size: 429 1104 2394 1348 3507
Fragments arranged by size:
3507 2394 1348 1104 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 7277 7451 65
Size: 1183 6029 174 1396
Fragments arranged by size:
6029 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3391 4056 4217 4471 5404 7426 7613
Size: 110 1581 665 161 254 933 2022 187
Cuts at: 7613 1700
Size: 2869
Fragments arranged by size:
2869 2022 1581 933 665 254 187 161
110
BsrGI T'GTAC_A
Cuts at: 96 2007 96
Size: 1911 6871
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8782
BssSI C'ACGA_G
Cuts at: 2115 2818 4858 6842 7854 2115
Size: 703 2040 1984 1012 3043
Fragments arranged by size:
3043 2040 1984 1012 703
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8782
BstBI TT'CG_AA
Cuts at: 5607 7586 5607
Size: 1979 6803
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8782
BstXI CCAn_nnnn'nTGG
Cuts at: 2419 3898 4376 4785 5590 2419
Size: 1479 478 409 805 5611
Fragments arranged by size:
5611 1479 805 478 409
BstYI r'GATC_y
Cuts at: 1972 3955 5268 6112 6479 6864 6881 8333 8419
Size: 1983 1313 844 367 385 17 1452 86
Cuts at: 8419 8431 8777 1972
Size: 12 346 1977
Fragments arranged by size:
1983 1977 1452 1313 844 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3216 5514 3216
Size: 2298 6484
BtgI C'CryG_G
Cuts at: 513 1065 2184 2239 2462 3290 4314 4369 4422
Size: 552 1119 55 223 828 1024 55 53
Cuts at: 4422 4648 513
Size: 226 4647
Fragments arranged by size:
4647 1119 1024 828 552 226 223 55
55 53
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8043
Fragments arranged by size:
8043 683 56
BtsI GCAGTG_nn'
Cuts at: 2696 2941 3246 3378 4585 4621 4964 5929 6298
Size: 245 305 132 1207 36 343 965 369
Cuts at: 6298 7092 7112 2696
Size: 794 20 4366
Fragments arranged by size:
4366 1207 965 794 369 343 305 245
132 36 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8782
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8747
DraI TTT'AAA
Cuts at: 3307 6086 3307
Size: 2779 6003
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2969 4309 4936 5546 7789 809
Size: 507 1653 1340 627 610 2243 1802
Fragments arranged by size:
2243 1802 1653 1340 627 610 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3480 4046 4771
Size: 54 1425 50 275 18 1650 566 725
Cuts at: 4771 6229 7118 8747 8
Size: 1458 889 1629 43
Fragments arranged by size:
1650 1629 1458 1425 889 725 566 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6229 1537
Size: 275 4417 4090
Fragments arranged by size:
4417 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2350 2828 4530 4720 5376 5982 6014 6712
Size: 997 478 1702 190 656 606 32 698
Cuts at: 6712 1353
Size: 3423
Fragments arranged by size:
3423 1702 997 698 656 606 478 190
32
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3021 4610 7369 7743 7889 387
Size: 1064 138 1432 1589 2759 374 146 1280
Fragments arranged by size:
2759 1589 1432 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2986 4528 5802 2986
Size: 1542 1274 5966
Fragments arranged by size:
5966 1542 1274
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2426 3000 3150 3352 3920 4514 4771
Size: 393 794 574 150 202 568 594 257
Cuts at: 4771 4901 5124 5623 5815 5965 6845 7442 8229
Size: 130 223 499 192 150 880 597 787
Cuts at: 8229 8497 1239
Size: 268 1524
Fragments arranged by size:
1524 880 794 787 597 594 574 568
499 393 268 257 223 202 192 150
150 130
EcoNI CCTnn'n_nnAGG
Cuts at: 3165 3165
Size: 8782
EcoO109I rG'GnC_Cy
Cuts at: 2128 2534 2877 3170 3910 4266 4427 2128
Size: 406 343 293 740 356 161 6483
Fragments arranged by size:
6483 740 406 356 343 293 161
EcoRI G'AATT_C
Cuts at: 2668 2824 6091 6469 2668
Size: 156 3267 378 4981
Fragments arranged by size:
4981 3267 378 156
FspI TGC'GCA
Cuts at: 2800 7288 2800
Size: 4488 4294
HaeII r_GCGC'y
Cuts at: 1678 2095 7929 1678
Size: 417 5834 2531
Fragments arranged by size:
5834 2531 417
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2443 2475 3341 3373 3459
Size: 32 135 32 871 32 866 32 86
Cuts at: 3459 3491 4724 4756 5135 5167 5375 5407 5963
Size: 32 1233 32 379 32 208 32 556
Cuts at: 5963 5995 6013 6045 7423 7455 7497 7529 1373
Size: 32 18 32 1378 32 42 32 2626
Fragments arranged by size:
2626 1378 1233 871 866 556 379 208
135 86 42 32 32 32 32 32
32 32 32 32 32 32 32 18
HincII GTy'rAC
Cuts at: 669 1657 1938 3119 6126 6372 669
Size: 988 281 1181 3007 246 3079
Fragments arranged by size:
3079 3007 1181 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3344 748
Size: 2596 6186
HpaI GTT'AAC
Cuts at: 6372 6372
Size: 8782
KasI G'GCGC_C
Cuts at: 2091 2091
Size: 8782
KpnI G_GTAC'C
Cuts at: 6107 6107
Size: 8782
MfeI C'AATT_G
Cuts at: 6381 6381
Size: 8782
MluI A'CGCG_T
Cuts at: 8658 8658
Size: 8782
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2452 3155 7896 8080 1305
Size: 1147 703 4741 184 2007
Fragments arranged by size:
4741 2007 1147 703 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4048 4773 10
Size: 54 1425 343 2216 725 4019
Fragments arranged by size:
4019 2216 1425 725 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2148 2202 3098 4779 5282 5532 6740
Size: 648 982 54 896 1681 503 250 1208
Cuts at: 6740 7099 7258 518
Size: 359 159 2042
Fragments arranged by size:
2042 1681 1208 982 896 648 503 359
250 159 54
MspA1I CmG'CkG
Cuts at: 1606 2186 3153 3561 4114 4650 4985 6198 6875
Size: 580 967 408 553 536 335 1213 677
Cuts at: 6875 8023 8268 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1213 1148 967 677 580 553 536
408 335 245
NaeI GCC'GGC
Cuts at: 3393 3393
Size: 8782
NarI GG'CG_CC
Cuts at: 2092 2092
Size: 8782
NcoI C'CATG_G
Cuts at: 513 1065 2462 513
Size: 552 1397 6833
Fragments arranged by size:
6833 1397 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8782
NgoMIV G'CCGG_C
Cuts at: 3391 3391
Size: 8782
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8782
NotI GC'GGCC_GC
Cuts at: 6229 6229
Size: 8782
NspI r_CATG'y
Cuts at: 1132 1229 2201 2492 2663 3886 6140 7685 1132
Size: 97 972 291 171 1223 2254 1545 2229
Fragments arranged by size:
2254 2229 1545 1223 972 291 171 97
PasI CC'CwG_GG
Cuts at: 1348 3322 4388 4488 5168 5418 1348
Size: 1974 1066 100 680 250 4712
Fragments arranged by size:
4712 1974 1066 680 250 100
PciI A'CATG_T
Cuts at: 2197 2659 7681 2197
Size: 462 5022 3298
Fragments arranged by size:
5022 3298 462
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2959 5944 1108
Size: 1851 2985 3946
Fragments arranged by size:
3946 2985 1851
PfoI T'CCnGG_A
Cuts at: 4530 4530
Size: 8782
PmeI GTTT'AAAC
Cuts at: 6086 6086
Size: 8782
PmlI CAC'GTG
Cuts at: 2449 4781 2449
Size: 2332 6450
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5585 5617 6851 6883 8364 8396 5585
Size: 32 1234 32 1481 32 5971
Fragments arranged by size:
5971 1481 1234 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2534 4266 2534
Size: 1732 7050
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8782
PsiI TTA'TAA
Cuts at: 6352 6352
Size: 8782
PspOMI G'GGCC_C
Cuts at: 4426 4426
Size: 8782
PstI C_TGCA'G
Cuts at: 830 2136 3909 4628 4767 6134 830
Size: 1306 1773 719 139 1367 3478
Fragments arranged by size:
3478 1773 1367 1306 719 139
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8782
PvuII CAG'CTG
Cuts at: 4114 4985 4114
Size: 871 7911
SacI G_AGCT'C
Cuts at: 2988 4530 5804 2988
Size: 1542 1274 5966
Fragments arranged by size:
5966 1542 1274
SacII CC_GC'GG
Cuts at: 2187 4651 2187
Size: 2464 6318
SalI G'TCGA_C
Cuts at: 1936 6124 1936
Size: 4188 4594
SapI GCTCTTCn'nnn_
Cuts at: 6014 6014
Size: 8782
SbfI CC_TGCA'GG
Cuts at: 6134 6134
Size: 8782
ScaI AGT'ACT
Cuts at: 7030 8742 7030
Size: 1712 7070
SfcI C'TryA_G
Cuts at: 826 1045 2132 3905 4624 4763 6130 7265 7946
Size: 219 1087 1773 719 139 1367 1135 681
Cuts at: 7946 8137 826
Size: 191 1471
Fragments arranged by size:
1773 1471 1367 1135 1087 719 681 219
191 139
SfoI GGC'GCC
Cuts at: 2093 2093
Size: 8782
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8782
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8782
SmaI CCC'GGG
Cuts at: 1872 3520 4800 5901 6109 1872
Size: 1648 1280 1101 208 4545
Fragments arranged by size:
4545 1648 1280 1101 208
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2882 3384 4028 4297 6886 7787
Size: 197 926 939 502 644 269 2589 901
Cuts at: 7787 8049 820
Size: 262 1553
Fragments arranged by size:
2589 1553 939 926 901 644 502 269
262 197
SnaBI TAC'GTA
Cuts at: 493 6573 493
Size: 6080 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8782
SphI G_CATG'C
Cuts at: 1132 6140 1132
Size: 5008 3774
SspI AAT'ATT
Cuts at: 5 52 6706 5
Size: 47 6654 2081
Fragments arranged by size:
6654 2081 47
StuI AGG'CCT
Cuts at: 1517 3471 5907 1517
Size: 1954 2436 4392
Fragments arranged by size:
4392 2436 1954
StyI C'CwwG_G
Cuts at: 513 1065 2085 2462 2579 4991 5589 5756 5967
Size: 552 1020 377 117 2412 598 167 211
Cuts at: 5967 6221 513
Size: 254 3074
Fragments arranged by size:
3074 2412 1020 598 552 377 254 211
167 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3149 3917 4684 4791 5198 6809 6826
Size: 1255 1569 768 767 107 407 1611 17
Cuts at: 6826 6979 7164 7609 325
Size: 153 185 445 1498
Fragments arranged by size:
1611 1569 1498 1255 768 767 445 407
185 153 107 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2007 3270 7028
Size: 274 80 33 51 1443 30 1263 3758
Cuts at: 7028 8740 96
Size: 1712 138
Fragments arranged by size:
3758 1712 1443 1263 274 138 80 51
33 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2646 2678 2725 2757 5895 5927 8742 8774 2646
Size: 32 47 32 3138 32 2815 32 2654
Fragments arranged by size:
3138 2815 2654 47 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5564 1316
Size: 4248 4534
XbaI T'CTAG_A
Cuts at: 6118 6118
Size: 8782
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8782
XmaI C'CCGG_G
Cuts at: 1870 3518 4798 5899 6107 1870
Size: 1648 1280 1101 208 4545
Fragments arranged by size:
4545 1648 1280 1101 208
XmnI GAAnn'nnTTC
Cuts at: 4939 5710 6911 4939
Size: 771 1201 6810
Fragments arranged by size:
6810 1201 771
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2181 6590 276
Size: 53 83 186 936 647 4409 2468
Fragments arranged by size:
4409 2468 936 647 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BfrBI BsiWI BsmBI BstZ17I DraIII
EcoRV FalI FseI FspAI NruI NsiI PacI PspXI
PsrI RsrII SanDI SexAI SfiI SrfI SwaI XcmI