Restriction map for
pFN21AA1457
(Circular) (Six-base) MAPSORT of: pFN21AA1457.seq Check: 7295 from: 1 to: 8734
pFN21AA1457 8734 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5598 5871 5598
Size: 273 8461
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6544 278
Size: 53 83 186 936 5008 2468
Fragments arranged by size:
5008 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3942 4269 5226 6077 1937
Size: 2005 327 957 851 4594
Fragments arranged by size:
4594 2005 957 851 327
Acc65I G'GTAC_C
Cuts at: 6055 6055
Size: 8734
AclI AA'CG_TT
Cuts at: 1563 6861 7234 1563
Size: 5298 373 3063
Fragments arranged by size:
5298 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 5299 5585 5771 6797 8181 8449 1632
Size: 3667 286 186 1026 1384 268 1917
Fragments arranged by size:
3667 1917 1384 1026 286 268 186
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8537
AflIII A'CryG_T
Cuts at: 1203 2376 3366 5259 7633 8610 1203
Size: 1173 990 1893 2374 977 1327
Fragments arranged by size:
2374 1893 1327 1173 990 977
AgeI A'CCGG_T
Cuts at: 7565 7565
Size: 8734
AhdI GACnn_n'nnGTC
Cuts at: 3799 7463 3799
Size: 3664 5070
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8734
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3018 3847 3850 5819 1184
Size: 1834 829 3 1969 4099
Fragments arranged by size:
4099 1969 1834 829 3
AlwNI CAG_nnn'CTG
Cuts at: 3247 4827 5044 5413 6477 8049 3247
Size: 1580 217 369 1064 1572 3932
Fragments arranged by size:
3932 1580 1572 1064 369 217
ApaI G_GGCC'C
Cuts at: 2517 5070 5898 6029 2517
Size: 2553 828 131 5222
Fragments arranged by size:
5222 2553 828 131
ApaLI G'TGCA_C
Cuts at: 3389 5108 5597 6791 7947 3389
Size: 1719 489 1194 1156 4176
Fragments arranged by size:
4176 1719 1194 1156 489
ApoI r'AATT_y
Cuts at: 1493 2321 6043 6269 6421 8530 1493
Size: 828 3722 226 152 2109 1697
Fragments arranged by size:
3722 2109 1697 828 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7288 160
Size: 872 6256 1606
Fragments arranged by size:
6256 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8734
AvaI C'yCGr_G
Cuts at: 1870 1943 3805 4537 6059 1870
Size: 73 1862 732 1522 4545
Fragments arranged by size:
4545 1862 1522 732 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5145 5178 5145
Size: 33 8701
BamHI G'GATC_C
Cuts at: 6064 6431 6064
Size: 367 8367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3176 3299 3317 3430 3921
Size: 325 217 627 1389 123 18 113 491
Cuts at: 3921 4133 4182 4595 6055 7510 618
Size: 212 49 413 1460 1455 1842
Fragments arranged by size:
1842 1460 1455 1389 627 491 413 325
217 212 123 113 49 18
BanII G_rGCy'C
Cuts at: 1391 2517 2674 2814 2856 2865 2886 3026 3107
Size: 1126 157 140 42 9 21 140 81
Cuts at: 3107 3137 4089 5070 5898 6029 6053 1391
Size: 30 952 981 828 131 24 4072
Fragments arranged by size:
4072 1126 981 952 828 157 140 140
131 81 42 30 24 21 9
BbeI G_GCGC'C
Cuts at: 3321 4599 3321
Size: 1278 7456
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2548 3153 4220 5305 928
Size: 971 649 605 1067 1085 4357
Fragments arranged by size:
4357 1085 1067 971 649 605
BbvCI CC'TCA_GC
Cuts at: 3479 4076 3479
Size: 597 8137
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5287 5321 6925 6959 1903
Size: 34 3350 34 1604 34 3678
Fragments arranged by size:
3678 3350 1604 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4018 5146 6626 7836 4018
Size: 1128 1480 1210 4916
Fragments arranged by size:
4916 1480 1210 1128
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8734
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2163 3765 4519
Size: 107 122 71 631 605 491 1602 754
Cuts at: 4519 4781 5741 5829 5893 7345 136
Size: 262 960 88 64 1452 1525
Fragments arranged by size:
1602 1525 1452 960 754 631 605 491
262 122 107 88 71 64
BglII A'GATC_T
Cuts at: 5664 8729 5664
Size: 3065 5669
BlpI GC'TnA_GC
Cuts at: 2714 6151 2714
Size: 3437 5297
Bme1580I G_kGCm'C
Cuts at: 1790 2451 2517 3181 3393 3435 4138 4187 4616
Size: 661 66 664 212 42 703 49 429
Cuts at: 4616 5070 5112 5300 5601 5898 6029 6795 7951
Size: 454 42 188 301 297 131 766 1156
Cuts at: 7951 1790
Size: 2573
Fragments arranged by size:
2573 1156 766 703 664 661 454 429
301 297 212 188 131 66 49 42
42
BmgBI CAC'GTC
Cuts at: 1318 2377 4843 5392 5626 1318
Size: 1059 2466 549 234 4426
Fragments arranged by size:
4426 2466 1059 549 234
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2614 2773 3565
Size: 416 53 83 399 24 1197 159 792
Cuts at: 3565 4837 5100 6465 7423 442
Size: 1272 263 1365 958 1753
Fragments arranged by size:
1753 1365 1272 1197 958 792 416 399
263 159 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 5343 1056
Size: 4287 4447
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2341 4108 2341
Size: 1767 6967
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2153 2382 2759 3184 3886 3929 4331 4463
Size: 914 229 377 425 702 43 402 132
Cuts at: 4463 4547 4870 6026 7394 1239
Size: 84 323 1156 1368 2579
Fragments arranged by size:
2579 1368 1156 914 702 425 402 377
323 229 132 84 43
Bpu10I CC'TnA_GC
Cuts at: 3479 3926 4076 4431 3479
Size: 447 150 355 7782
Fragments arranged by size:
7782 447 355 150
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2888 4228 6859 7724 8022 2888
Size: 1340 2631 865 298 3600
Fragments arranged by size:
3600 2631 1340 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3475 4406 4852 5247 7397 882
Size: 617 1976 931 446 395 2150 2219
Fragments arranged by size:
2219 2150 1976 931 617 446 395
BsaAI yAC'GTr
Cuts at: 493 1204 3274 4972 5434 6525 493
Size: 711 2070 1698 462 1091 2702
Fragments arranged by size:
2702 2070 1698 1091 711 462
BsaBI GATnn'nnATC
Cuts at: 4658 5669 8728 4658
Size: 1011 3059 4664
Fragments arranged by size:
4664 3059 1011
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3318 3456 4596 4773
Size: 53 83 186 936 1785 138 1140 177
Cuts at: 4773 6541 6923 7558 275
Size: 1768 382 635 1451
Fragments arranged by size:
1785 1768 1451 1140 936 635 382 186
177 138 83 53
BsaWI w'CCGG_w
Cuts at: 2645 5001 7167 7565 7839 7986 8397 2645
Size: 2356 2166 398 274 147 411 2982
Fragments arranged by size:
2982 2356 2166 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3317 3347 4832 4862 1178
Size: 30 165 30 1914 30 1485 30 5050
Fragments arranged by size:
5050 1914 1485 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2063 2276 2552 2813 3553 4012 4594 1186
Size: 877 213 276 261 740 459 582 5326
Fragments arranged by size:
5326 877 740 582 459 276 261 213
BseYI C'CCAG_C
Cuts at: 2064 2213 2505 2864 3329 3398 4189 4225 4381
Size: 149 292 359 465 69 791 36 156
Cuts at: 4381 5121 5484 5883 5888 5897 5979 7937 8433
Size: 740 363 399 5 9 82 1958 496
Cuts at: 8433 2064
Size: 2365
Fragments arranged by size:
2365 1958 791 740 496 465 399 363
359 292 156 149 82 69 36 9
5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3237 3275 4439 5570 1163
Size: 573 1501 38 1164 1131 4327
Fragments arranged by size:
4327 1501 1164 1131 573 38
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2144 2942 4253 5973 6184 6945
Size: 275 186 143 798 1311 1720 211 761
Cuts at: 6945 7973 1540
Size: 1028 2301
Fragments arranged by size:
2301 1720 1311 1028 798 761 275 211
186 143
BsiHKAI G_wGCw'C
Cuts at: 2814 3026 3107 3393 4563 5112 5601 6795 6880
Size: 212 81 286 1170 549 489 1194 85
Cuts at: 6880 7951 2814
Size: 1071 3597
Fragments arranged by size:
3597 1194 1170 1071 549 489 286 212
85 81
BsmI GAATG_Cn'
Cuts at: 2278 3826 6245 6338 2278
Size: 1548 2419 93 4674
Fragments arranged by size:
4674 2419 1548 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2836 2940 2836
Size: 104 8630
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2451 2517 2674 2814 2856 2865 2886
Size: 399 661 66 157 140 42 9 21
Cuts at: 2886 3026 3107 3137 3181 3393 3435 4089 4138
Size: 140 81 30 44 212 42 654 49
Cuts at: 4138 4187 4563 4616 5070 5112 5300 5601 5898
Size: 49 376 53 454 42 188 301 297
Cuts at: 5898 6029 6053 6795 6880 7951 1391
Size: 131 24 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 661 654 454 399 376
301 297 212 188 157 140 140 131
85 81 66 53 49 49 44 42
42 42 30 24 21 9
BspEI T'CCGG_A
Cuts at: 8397 8397
Size: 8734
BspHI T'CATG_A
Cuts at: 4869 5115 6623 8353 4869
Size: 246 1508 1730 5250
Fragments arranged by size:
5250 1730 1508 246
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2542 3655 3804 4134 5043 5598 5871 6089
Size: 1698 1113 149 330 909 555 273 218
Cuts at: 6089 844
Size: 3489
Fragments arranged by size:
3489 1698 1113 909 555 330 273 218
149
BsrBI CCG'CTC
Cuts at: 1394 1823 2226 5777 5914 6621 1394
Size: 429 403 3551 137 707 3507
Fragments arranged by size:
3551 3507 707 429 403 137
BsrDI GCAATG_nn'
Cuts at: 65 1248 2881 7229 7403 65
Size: 1183 1633 4348 174 1396
Fragments arranged by size:
4348 1633 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2139 2676 3733 4252 5442 5679 5904
Size: 110 329 537 1057 519 1190 237 225
Cuts at: 5904 5972 7378 7565 1700
Size: 68 1406 187 2869
Fragments arranged by size:
2869 1406 1190 1057 537 519 329 237
225 187 110 68
BsrGI T'GTAC_A
Cuts at: 96 2070 5634 96
Size: 1974 3564 3196
Fragments arranged by size:
3564 3196 1974
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8734
BssSI C'ACGA_G
Cuts at: 3050 5909 6794 7806 3050
Size: 2859 885 1012 3978
Fragments arranged by size:
3978 2859 1012 885
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3710 794
Size: 2916 5818
BstBI TT'CG_AA
Cuts at: 7538 7538
Size: 8734
BstEII G'GTnAC_C
Cuts at: 1184 5080 5161 1184
Size: 3896 81 4757
Fragments arranged by size:
4757 3896 81
BstXI CCAn_nnnn'nTGG
Cuts at: 2846 3406 3427 4120 4405 4592 4894 5626 5797
Size: 560 21 693 285 187 302 732 171
Cuts at: 5797 5933 2846
Size: 136 5647
Fragments arranged by size:
5647 732 693 560 302 285 187 171
136 21
BstYI r'GATC_y
Cuts at: 1972 2127 3267 5664 6064 6431 6816 6833 8285
Size: 155 1140 2397 400 367 385 17 1452
Cuts at: 8285 8371 8383 8729 1972
Size: 86 12 346 1977
Fragments arranged by size:
2397 1977 1452 1140 400 385 367 346
155 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 5131 5131
Size: 8734
BtgI C'CryG_G
Cuts at: 513 1065 2043 4287 4471 4791 5385 5500 6021
Size: 552 978 2244 184 320 594 115 521
Cuts at: 6021 513
Size: 3226
Fragments arranged by size:
3226 2244 978 594 552 521 320 184
115
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2044 3343 3360 4645 4693 487
Size: 683 56 818 1299 17 1285 48 4528
Fragments arranged by size:
4528 1299 1285 818 683 56 48 17
BtsI GCAGTG_nn'
Cuts at: 3829 3880 4614 5711 6250 7044 7064 3829
Size: 51 734 1097 539 794 20 5499
Fragments arranged by size:
5499 1097 794 734 539 51 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8734
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2025 2060 4824 4859 5155 5190 529
Size: 35 1461 35 2764 35 296 35 4073
Fragments arranged by size:
4073 2764 1461 296 35 35 35 35
DraI TTT'AAA
Cuts at: 6038 6038
Size: 8734
DraIII CAC_nnn'GTG
Cuts at: 2188 3099 2188
Size: 911 7823
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7741 809
Size: 507 6425 1802
Fragments arranged by size:
6425 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2141 3555 4250
Size: 54 1425 50 275 18 311 1414 695
Cuts at: 4250 4992 5787 5830 5970 5998 6181 7070 8699
Size: 742 795 43 140 28 183 889 1629
Cuts at: 8699 8
Size: 43
Fragments arranged by size:
1629 1425 1414 889 795 742 695 311
275 183 140 54 50 43 43 28
18
EagI C'GGCC_G
Cuts at: 1537 1812 2141 4250 5970 6181 1537
Size: 275 329 2109 1720 211 4090
Fragments arranged by size:
4090 2109 1720 329 275 211
EarI CTCTTCn'nnn_
Cuts at: 1353 2081 2466 4175 4181 4283 4817 6664 1353
Size: 728 385 1709 6 102 534 1847 3423
Fragments arranged by size:
3423 1847 1709 728 534 385 102 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3413 4628 5945 7321 7695 7841
Size: 1064 138 1824 1215 1317 1376 374 146
Cuts at: 7841 387
Size: 1280
Fragments arranged by size:
1824 1376 1317 1280 1215 1064 374 146
138
EcoICRI GAG'CTC
Cuts at: 2812 3024 3105 2812
Size: 212 81 8441
Fragments arranged by size:
8441 212 81
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2153 2382 2759 3184 3886 3929 4331
Size: 393 521 229 377 425 702 43 402
Cuts at: 4331 4463 4547 4870 5299 5585 5771 6026 6797
Size: 132 84 323 429 286 186 255 771
Cuts at: 6797 7394 8181 8449 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 787 771 702 597 521 429 425
402 393 377 323 286 268 255 229
186 132 84 43
EcoNI CCTnn'n_nnAGG
Cuts at: 2237 2237
Size: 8734
EcoO109I rG'GnC_Cy
Cuts at: 2476 2513 2514 3055 3325 3970 5067 5410 6026
Size: 37 1 541 270 645 1097 343 616
Cuts at: 6026 2476
Size: 5184
Fragments arranged by size:
5184 1097 645 616 541 343 270 37
1
EcoRI G'AATT_C
Cuts at: 6043 6421 6043
Size: 378 8356
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2572 5575 5833 2572
Size: 3003 258 5473
Fragments arranged by size:
5473 3003 258
FseI GG_CCGG'CC
Cuts at: 4256 5446 5683 4256
Size: 1190 237 7307
Fragments arranged by size:
7307 1190 237
FspI TGC'GCA
Cuts at: 2572 3048 5807 7240 2572
Size: 476 2759 1433 4066
Fragments arranged by size:
4066 2759 1433 476
FspAI rTGC'GCAy
Cuts at: 2572 3048 2572
Size: 476 8258
HaeII r_GCGC'y
Cuts at: 1678 3321 3405 4333 4599 5643 5804 7881 1678
Size: 1643 84 928 266 1044 161 2077 2531
Fragments arranged by size:
2531 2077 1643 1044 928 266 161 84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2828 2860 3008 3040 3044
Size: 32 135 32 1256 32 148 32 4
Cuts at: 3044 3076 3288 3317 3320 3349 3785 3817 4379
Size: 32 212 29 3 29 436 32 562
Cuts at: 4379 4411 4832 4864 7375 7407 7449 7481 1373
Size: 32 421 32 2511 32 42 32 2626
Fragments arranged by size:
2626 2511 1256 562 436 421 212 148
135 42 32 32 32 32 32 32
32 32 32 32 29 29 4 3
HincII GTy'rAC
Cuts at: 669 1657 1938 2496 3943 4836 6078 6324 669
Size: 988 281 558 1447 893 1242 246 3079
Fragments arranged by size:
3079 1447 1242 988 893 558 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8734
HpaI GTT'AAC
Cuts at: 6324 6324
Size: 8734
KasI G'GCGC_C
Cuts at: 3317 4595 3317
Size: 1278 7456
KpnI G_GTAC'C
Cuts at: 6059 6059
Size: 8734
MfeI C'AATT_G
Cuts at: 6333 6333
Size: 8734
MluI A'CGCG_T
Cuts at: 8610 8610
Size: 8734
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3087 3498 4533 7848 8032 1305
Size: 1782 411 1035 3315 184 2007
Fragments arranged by size:
3315 2007 1782 1035 411 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3557 10
Size: 54 1425 343 1725 5187
Fragments arranged by size:
5187 1725 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3380 3386 3815 5282 5432 5795 5963
Size: 648 2214 6 429 1467 150 363 168
Cuts at: 5963 6692 7051 7210 518
Size: 729 359 159 2042
Fragments arranged by size:
2214 2042 1467 729 648 429 363 359
168 159 150 6
MspA1I CmG'CkG
Cuts at: 1606 2068 2118 2217 2410 2509 3214 3247 4189
Size: 462 50 99 193 99 705 33 942
Cuts at: 4189 4246 4289 4318 4381 5464 5542 5749 5892
Size: 57 43 29 63 1083 78 207 143
Cuts at: 5892 5944 6023 6150 6827 7975 8220 1606
Size: 52 79 127 677 1148 245 2120
Fragments arranged by size:
2120 1148 1083 942 705 677 462 245
207 193 143 127 99 99 79 78
63 57 52 50 43 33 29
NaeI GCC'GGC
Cuts at: 4254 5444 5681 5974 4254
Size: 1190 237 293 7014
Fragments arranged by size:
7014 1190 293 237
NarI GG'CG_CC
Cuts at: 3318 4596 3318
Size: 1278 7456
NcoI C'CATG_G
Cuts at: 513 1065 5500 513
Size: 552 4435 3747
Fragments arranged by size:
4435 3747 552
NdeI CA'TA_TG
Cuts at: 387 2104 387
Size: 1717 7017
NgoMIV G'CCGG_C
Cuts at: 4252 5442 5679 5972 4252
Size: 1190 237 293 7014
Fragments arranged by size:
7014 1190 293 237
NheI G'CTAG_C
Cuts at: 1052 5339 1052
Size: 4287 4447
NotI GC'GGCC_GC
Cuts at: 6181 6181
Size: 8734
NspI r_CATG'y
Cuts at: 1132 1229 2206 2614 3784 5455 6092 7637 1132
Size: 97 977 408 1170 1671 637 1545 2229
Fragments arranged by size:
2229 1671 1545 1170 977 637 408 97
PasI CC'CwG_GG
Cuts at: 1348 3406 4138 1348
Size: 2058 732 5944
Fragments arranged by size:
5944 2058 732
PciI A'CATG_T
Cuts at: 7633 7633
Size: 8734
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3247 4827 5506 1108
Size: 2139 1580 679 4336
Fragments arranged by size:
4336 2139 1580 679
PfoI T'CCnGG_A
Cuts at: 2130 2730 3306 4440 4485 5852 2130
Size: 600 576 1134 45 1367 5012
Fragments arranged by size:
5012 1367 1134 600 576 45
PmeI GTTT'AAAC
Cuts at: 6038 6038
Size: 8734
PmlI CAC'GTG
Cuts at: 3274 4972 3274
Size: 1698 7036
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3062 3094 6803 6835 8316 8348 3062
Size: 32 3709 32 1481 32 3448
Fragments arranged by size:
3709 3448 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2476 3055 3325 3970 5410 2476
Size: 579 270 645 1440 5800
Fragments arranged by size:
5800 1440 645 579 270
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8734
PsiI TTA'TAA
Cuts at: 2012 2349 6304 2012
Size: 337 3955 4442
Fragments arranged by size:
4442 3955 337
PspOMI G'GGCC_C
Cuts at: 2513 5066 5894 6025 2513
Size: 2553 828 131 5222
Fragments arranged by size:
5222 2553 828 131
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2129 2161 2129
Size: 32 8702
PstI C_TGCA'G
Cuts at: 830 5700 6086 830
Size: 4870 386 3478
Fragments arranged by size:
4870 3478 386
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8734
PvuII CAG'CTG
Cuts at: 2068 2217 2410 3214 4246 5749 2068
Size: 149 193 804 1032 1503 5053
Fragments arranged by size:
5053 1503 1032 804 193 149
SacI G_AGCT'C
Cuts at: 2814 3026 3107 2814
Size: 212 81 8441
Fragments arranged by size:
8441 212 81
SacII CC_GC'GG
Cuts at: 4290 6024 4290
Size: 1734 7000
SalI G'TCGA_C
Cuts at: 1936 3941 6076 1936
Size: 2005 2135 4594
Fragments arranged by size:
4594 2135 2005
SanDI GG'GwC_CC
Cuts at: 3325 3325
Size: 8734
SapI GCTCTTCn'nnn_
Cuts at: 2081 2081
Size: 8734
SbfI CC_TGCA'GG
Cuts at: 6086 6086
Size: 8734
ScaI AGT'ACT
Cuts at: 6982 8694 6982
Size: 1712 7022
SexAI A'CCwGG_T
Cuts at: 2294 2294
Size: 8734
SfcI C'TryA_G
Cuts at: 826 1045 3487 3721 5696 6082 7217 7898 8089
Size: 219 2442 234 1975 386 1135 681 191
Cuts at: 8089 826
Size: 1471
Fragments arranged by size:
2442 1975 1471 1135 681 386 234 219
191
SfiI GGCCn_nnn'nGGCC
Cuts at: 5829 5829
Size: 8734
SfoI GGC'GCC
Cuts at: 3319 4597 3319
Size: 1278 7456
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8734
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8734
SmaI CCC'GGG
Cuts at: 1872 3807 4539 6061 1872
Size: 1935 732 1522 4545
Fragments arranged by size:
4545 1935 1522 732
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2903 4207 6838 7739 8001 820
Size: 197 926 960 1304 2631 901 262 1553
Fragments arranged by size:
2631 1553 1304 960 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6525 493
Size: 6032 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8734
SphI G_CATG'C
Cuts at: 1132 2206 2614 3784 6092 1132
Size: 1074 408 1170 2308 3774
Fragments arranged by size:
3774 2308 1170 1074 408
SrfI GCCC'GGGC
Cuts at: 4539 4539
Size: 8734
SspI AAT'ATT
Cuts at: 5 52 6658 5
Size: 47 6606 2081
Fragments arranged by size:
6606 2081 47
StuI AGG'CCT
Cuts at: 1517 2684 4046 1517
Size: 1167 1362 6205
Fragments arranged by size:
6205 1362 1167
StyI C'CwwG_G
Cuts at: 513 1065 2238 3084 4940 5292 5367 5500 5619
Size: 552 1173 846 1856 352 75 133 119
Cuts at: 5619 6030 6173 513
Size: 411 143 3074
Fragments arranged by size:
3074 1856 1173 846 552 411 352 143
133 119 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2928 5210 6761 6778 6931 7116 7561
Size: 1255 1348 2282 1551 17 153 185 445
Cuts at: 7561 325
Size: 1498
Fragments arranged by size:
2282 1551 1498 1348 1255 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2070 2175 2541
Size: 274 80 33 51 1443 93 105 366
Cuts at: 2541 4761 5634 5754 6980 8692 96
Size: 2220 873 120 1226 1712 138
Fragments arranged by size:
2220 1712 1443 1226 873 366 274 138
120 105 93 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8694 8726 8694
Size: 32 8702
Tth111I GACn'n_nGTC
Cuts at: 1316 2432 3377 3686 4844 5078 1316
Size: 1116 945 309 1158 234 4972
Fragments arranged by size:
4972 1158 1116 945 309 234
XbaI T'CTAG_A
Cuts at: 6070 6070
Size: 8734
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3115 4018 4947 5623 3115
Size: 903 929 676 6226
Fragments arranged by size:
6226 929 903 676
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8734
XmaI C'CCGG_G
Cuts at: 1870 3805 4537 6059 1870
Size: 1935 732 1522 4545
Fragments arranged by size:
4545 1935 1522 732
XmnI GAAnn'nnTTC
Cuts at: 6863 6863
Size: 8734
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6542 276
Size: 53 83 186 936 5008 2468
Fragments arranged by size:
5008 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BfrBI BsiWI BstZ17I EcoRV
NruI NsiI PacI PspXI RsrII SwaI