Restriction map for
pFN21AA1451
(Circular) (Six-base) MAPSORT of: pFN21AA1451.seq Check: 7707 from: 1 to: 7372
pFN21AA1451 7372 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3164 3164
Size: 7372
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5182 278
Size: 53 83 186 936 3646 2468
Fragments arranged by size:
3646 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4715 1937
Size: 2778 4594
Acc65I G'GTAC_C
Cuts at: 4693 4693
Size: 7372
AclI AA'CG_TT
Cuts at: 1563 5499 5872 1563
Size: 3936 373 3063
Fragments arranged by size:
3936 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2733 3225 3464 4125 5435 6819 7087 1632
Size: 1101 492 239 661 1310 1384 268 1917
Fragments arranged by size:
1917 1384 1310 1101 661 492 268 239
AflII C'TTAA_G
Cuts at: 820 1017 2366 820
Size: 197 1349 5826
Fragments arranged by size:
5826 1349 197
AflIII A'CryG_T
Cuts at: 1203 2875 3785 6271 7248 1203
Size: 1672 910 2486 977 1327
Fragments arranged by size:
2486 1672 1327 977 910
AgeI A'CCGG_T
Cuts at: 6203 6203
Size: 7372
AhdI GACnn_n'nnGTC
Cuts at: 6101 6101
Size: 7372
AleI CACnn'nnGTG
Cuts at: 1166 2876 2919 1166
Size: 1710 43 5619
Fragments arranged by size:
5619 1710 43
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7372
AlwNI CAG_nnn'CTG
Cuts at: 2116 3197 4409 5115 6687 2116
Size: 1081 1212 706 1572 2801
Fragments arranged by size:
2801 1572 1212 1081 706
ApaLI G'TGCA_C
Cuts at: 5429 6585 5429
Size: 1156 6216
ApoI r'AATT_y
Cuts at: 1493 3735 3818 4025 4681 4907 5059 7168 1493
Size: 2242 83 207 656 226 152 2109 1697
Fragments arranged by size:
2242 2109 1697 656 226 207 152 83
AseI AT'TA_AT
Cuts at: 160 1032 4007 5926 160
Size: 872 2975 1919 1606
Fragments arranged by size:
2975 1919 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7372
AvaI C'yCGr_G
Cuts at: 1870 1943 4077 4697 1870
Size: 73 2134 620 4545
Fragments arranged by size:
4545 2134 620 73
AvrII C'CTAG_G
Cuts at: 3838 3838
Size: 7372
BamHI G'GATC_C
Cuts at: 2699 4702 5069 2699
Size: 2003 367 5002
Fragments arranged by size:
5002 2003 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4693 6148 618
Size: 325 217 627 2906 1455 1842
Fragments arranged by size:
2906 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4691 1391
Size: 3300 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2801 928
Size: 971 902 5499
Fragments arranged by size:
5499 971 902
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5563 5597 1903
Size: 34 3626 34 3678
Fragments arranged by size:
3678 3626 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3620 3794 5264 6474 3620
Size: 174 1470 1210 4518
Fragments arranged by size:
4518 1470 1210 174
BclI T'GATC_A
Cuts at: 1548 2733 2826 3010 1548
Size: 1185 93 184 5910
Fragments arranged by size:
5910 1185 184 93
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4366 5983 136
Size: 107 122 71 631 605 2694 1617 1525
Fragments arranged by size:
2694 1617 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7367 7367
Size: 7372
BlpI GC'TnA_GC
Cuts at: 4789 4789
Size: 7372
Bme1580I G_kGCm'C
Cuts at: 1790 5433 6589 1790
Size: 3643 1156 2573
Fragments arranged by size:
3643 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7372
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3893 4493 5103
Size: 416 53 83 399 24 2476 600 610
Cuts at: 5103 6061 442
Size: 958 1753
Fragments arranged by size:
2476 1753 958 610 600 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7372
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3779 6032 1239
Size: 2540 2253 2579
Fragments arranged by size:
2579 2540 2253
Bpu10I CC'TnA_GC
Cuts at: 2852 3108 3599 2852
Size: 256 491 6625
Fragments arranged by size:
6625 491 256
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5497 6362 6660 5497
Size: 865 298 6209
Fragments arranged by size:
6209 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6035 882
Size: 617 4536 2219
Fragments arranged by size:
4536 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2900 5163 493
Size: 711 1696 2263 2702
Fragments arranged by size:
2702 2263 1696 711
BsaBI GATnn'nnATC
Cuts at: 2181 7366 2181
Size: 5185 2187
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5179 5561 6196 275
Size: 53 83 186 936 3646 382 635 1451
Fragments arranged by size:
3646 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5805 6203 6477 6624 7035 5805
Size: 398 274 147 411 6142
Fragments arranged by size:
6142 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7147
Fragments arranged by size:
7147 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4414 1186
Size: 3228 4144
BseYI C'CCAG_C
Cuts at: 6575 7071 6575
Size: 496 6876
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6799
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4822 5583 6611 1540
Size: 275 186 2821 761 1028 2301
Fragments arranged by size:
2821 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5433 5518 6589 5433
Size: 85 1071 6216
Fragments arranged by size:
6216 1071 85
BsmI GAATG_Cn'
Cuts at: 2590 4883 4976 2590
Size: 2293 93 4986
Fragments arranged by size:
4986 2293 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4691 5433 5518 6589 1391
Size: 399 2901 742 85 1071 2174
Fragments arranged by size:
2901 2174 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 7035 7035
Size: 7372
BspHI T'CATG_A
Cuts at: 5261 6991 5261
Size: 1730 5642
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3164 4727 844
Size: 2320 1563 3489
Fragments arranged by size:
3489 2320 1563
BsrBI CCG'CTC
Cuts at: 1394 1823 5259 1394
Size: 429 3436 3507
Fragments arranged by size:
3507 3436 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4004 5867 6041 65
Size: 1183 2756 1863 174 1396
Fragments arranged by size:
2756 1863 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4582 6016 6203 1700
Size: 110 2772 1434 187 2869
Fragments arranged by size:
2869 2772 1434 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7372
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7372
BssSI C'ACGA_G
Cuts at: 5432 6444 5432
Size: 1012 6360
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7372
BstBI TT'CG_AA
Cuts at: 4558 6176 4558
Size: 1618 5754
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7372
BstXI CCAn_nnnn'nTGG
Cuts at: 2555 2555
Size: 7372
BstYI r'GATC_y
Cuts at: 1972 2437 2699 4702 5069 5454 5471 6923 7009
Size: 465 262 2003 367 385 17 1452 86
Cuts at: 7009 7021 7367 1972
Size: 12 346 1977
Fragments arranged by size:
2003 1977 1452 465 385 367 346 262
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 4253 4587 513
Size: 552 939 2249 334 3298
Fragments arranged by size:
3298 2249 939 552 334
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6633
Fragments arranged by size:
6633 683 56
BtsI GCAGTG_nn'
Cuts at: 2676 4888 5682 5702 2676
Size: 2212 794 20 4346
Fragments arranged by size:
4346 2212 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7372
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7337
DraI TTT'AAA
Cuts at: 3126 3823 4676 3126
Size: 697 853 5822
Fragments arranged by size:
5822 853 697
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6379 809
Size: 507 5063 1802
Fragments arranged by size:
5063 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4584 4819 5708
Size: 54 1425 50 275 18 2754 235 889
Cuts at: 5708 7337 8
Size: 1629 43
Fragments arranged by size:
2754 1629 1425 889 275 235 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4819 1537
Size: 275 3007 4090
Fragments arranged by size:
4090 3007 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4173 4560 5302 1353
Size: 2820 387 742 3423
Fragments arranged by size:
3423 2820 742 387
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5959 6333 6479 387
Size: 1064 138 4370 374 146 1280
Fragments arranged by size:
4370 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2733 3225 3464 3779 4125 5435 6032
Size: 393 1101 492 239 315 346 1310 597
Cuts at: 6032 6819 7087 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1310 1101 787 597 492 393 346
315 268 239
EcoNI CCTnn'n_nnAGG
Cuts at: 3266 3353 3266
Size: 87 7285
EcoO109I rG'GnC_Cy
Cuts at: 4258 4258
Size: 7372
EcoRI G'AATT_C
Cuts at: 3735 4681 5059 3735
Size: 946 378 6048
Fragments arranged by size:
6048 946 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2087 2381 2641 3755 2087
Size: 294 260 1114 5704
Fragments arranged by size:
5704 1114 294 260
FspI TGC'GCA
Cuts at: 5878 5878
Size: 7372
HaeII r_GCGC'y
Cuts at: 1678 2143 6519 1678
Size: 465 4376 2531
Fragments arranged by size:
4376 2531 465
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3335 3367 4034 4066 6013
Size: 32 135 32 1763 32 667 32 1947
Cuts at: 6013 6045 6087 6119 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1947 1763 667 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3675 4716 4962 669
Size: 988 281 1737 1041 246 3079
Fragments arranged by size:
3079 1737 1041 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2154 748
Size: 1406 5966
HpaI GTT'AAC
Cuts at: 3675 4962 3675
Size: 1287 6085
KpnI G_GTAC'C
Cuts at: 4697 4697
Size: 7372
MfeI C'AATT_G
Cuts at: 4971 4971
Size: 7372
MluI A'CGCG_T
Cuts at: 7248 7248
Size: 7372
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2186 2677 4004 6486 6670 1305
Size: 881 491 1327 2482 184 2007
Fragments arranged by size:
2482 2007 1327 881 491 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5550
Fragments arranged by size:
5550 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2876 2919 5330 5689 5848 518
Size: 648 1710 43 2411 359 159 2042
Fragments arranged by size:
2411 2042 1710 648 359 159 43
MspA1I CmG'CkG
Cuts at: 1606 4788 5465 6613 6858 1606
Size: 3182 677 1148 245 2120
Fragments arranged by size:
3182 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 4253 513
Size: 552 939 2249 3632
Fragments arranged by size:
3632 2249 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7372
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7372
NotI GC'GGCC_GC
Cuts at: 4819 4819
Size: 7372
NspI r_CATG'y
Cuts at: 1132 1229 2879 3789 4730 6275 1132
Size: 97 1650 910 941 1545 2229
Fragments arranged by size:
2229 1650 1545 941 910 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7372
PciI A'CATG_T
Cuts at: 2875 3785 6271 2875
Size: 910 2486 3976
Fragments arranged by size:
3976 2486 910
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2505 2650 1108
Size: 1397 145 5830
Fragments arranged by size:
5830 1397 145
PfoI T'CCnGG_A
Cuts at: 3309 3309
Size: 7372
PmeI GTTT'AAAC
Cuts at: 4676 4676
Size: 7372
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5441 5473 6954 6986 5441
Size: 32 1481 32 5827
Fragments arranged by size:
5827 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4258 4258
Size: 7372
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7372
PsiI TTA'TAA
Cuts at: 2310 3456 4942 2310
Size: 1146 1486 4740
Fragments arranged by size:
4740 1486 1146
PspXI vC'TCGA_Gb
Cuts at: 4077 4077
Size: 7372
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2559 2591 2559
Size: 32 7340
PstI C_TGCA'G
Cuts at: 830 4724 830
Size: 3894 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7372
SalI G'TCGA_C
Cuts at: 1936 4714 1936
Size: 2778 4594
SanDI GG'GwC_CC
Cuts at: 4258 4258
Size: 7372
SapI GCTCTTCn'nnn_
Cuts at: 4560 4560
Size: 7372
SbfI CC_TGCA'GG
Cuts at: 4724 4724
Size: 7372
ScaI AGT'ACT
Cuts at: 5620 7332 5620
Size: 1712 5660
SfcI C'TryA_G
Cuts at: 826 1045 4720 5855 6536 6727 826
Size: 219 3675 1135 681 191 1471
Fragments arranged by size:
3675 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7372
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7372
SmaI CCC'GGG
Cuts at: 1872 4699 1872
Size: 2827 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2366 4077 5476 6377 6639 820
Size: 197 926 423 1711 1399 901 262 1553
Fragments arranged by size:
1711 1553 1399 926 901 423 262 197
SnaBI TAC'GTA
Cuts at: 493 5163 493
Size: 4670 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7372
SphI G_CATG'C
Cuts at: 1132 4730 1132
Size: 3598 3774
SspI AAT'ATT
Cuts at: 5 52 3654 3781 4546 5296 5
Size: 47 3602 127 765 750 2081
Fragments arranged by size:
3602 2081 765 750 127 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7372
StyI C'CwwG_G
Cuts at: 513 1065 2004 2071 2143 3267 3838 4253 4811
Size: 552 939 67 72 1124 571 415 558
Cuts at: 4811 513
Size: 3074
Fragments arranged by size:
3074 1124 939 571 558 552 415 72
67
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5399 5416 5569 5754 6199 325
Size: 1255 3819 17 153 185 445 1498
Fragments arranged by size:
3819 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2426 4457 5618
Size: 274 80 33 51 1443 449 2031 1161
Cuts at: 5618 7330 96
Size: 1712 138
Fragments arranged by size:
2031 1712 1443 1161 449 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7332 7364 7332
Size: 32 7340
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7372
XbaI T'CTAG_A
Cuts at: 4708 4708
Size: 7372
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3161 3161
Size: 7372
XhoI C'TCGA_G
Cuts at: 1943 4077 1943
Size: 2134 5238
XmaI C'CCGG_G
Cuts at: 1870 4697 1870
Size: 2827 4545
XmnI GAAnn'nnTTC
Cuts at: 5501 5501
Size: 7372
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5180 276
Size: 53 83 186 936 3646 2468
Fragments arranged by size:
3646 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspXI PsrI PstI PvuI SalI SanDI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PvuII RsrII SacI SacII SexAI
SfiI SfoI SrfI SwaI