Restriction map for
pFN21AA1450
(Circular) (Six-base) MAPSORT of: pFN21AA1450.seq Check: 4517 from: 1 to: 8047
pFN21AA1450 8047 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5857 278
Size: 53 83 186 936 4321 2468
Fragments arranged by size:
4321 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5390 1937
Size: 3453 4594
Acc65I G'GTAC_C
Cuts at: 5368 5368
Size: 8047
AclI AA'CG_TT
Cuts at: 1563 6174 6547 1563
Size: 4611 373 3063
Fragments arranged by size:
4611 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2910 3065 4630 4674 4707 4934 5001 5093
Size: 1278 155 1565 44 33 227 67 92
Cuts at: 5093 6110 7494 7762 1632
Size: 1017 1384 268 1917
Fragments arranged by size:
1917 1565 1384 1278 1017 268 227 155
92 67 44 33
AfeI AGC'GCT
Cuts at: 3251 3251
Size: 8047
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7850
AflIII A'CryG_T
Cuts at: 1203 2412 6946 7923 1203
Size: 1209 4534 977 1327
Fragments arranged by size:
4534 1327 1209 977
AgeI A'CCGG_T
Cuts at: 4092 6878 4092
Size: 2786 5261
AhdI GACnn_n'nnGTC
Cuts at: 6776 6776
Size: 8047
AleI CACnn'nnGTG
Cuts at: 1166 3518 4749 1166
Size: 2352 1231 4464
Fragments arranged by size:
4464 2352 1231
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8047
AlwNI CAG_nnn'CTG
Cuts at: 2541 4190 4924 5790 7362 2541
Size: 1649 734 866 1572 3226
Fragments arranged by size:
3226 1649 1572 866 734
ApaI G_GGCC'C
Cuts at: 4589 4589
Size: 8047
ApaLI G'TGCA_C
Cuts at: 6104 7260 6104
Size: 1156 6891
ApoI r'AATT_y
Cuts at: 1493 2986 3146 5356 5582 5734 7843 1493
Size: 1493 160 2210 226 152 2109 1697
Fragments arranged by size:
2210 2109 1697 1493 226 160 152
AseI AT'TA_AT
Cuts at: 160 1032 6601 160
Size: 872 5569 1606
Fragments arranged by size:
5569 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8047
AvaI C'yCGr_G
Cuts at: 1870 1943 5372 1870
Size: 73 3429 4545
Fragments arranged by size:
4545 3429 73
BamHI G'GATC_C
Cuts at: 5377 5744 5377
Size: 367 7680
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3900 3973 5368 6823 618
Size: 325 217 627 2113 73 1395 1455 1842
Fragments arranged by size:
2113 1842 1455 1395 627 325 217 73
BanII G_rGCy'C
Cuts at: 1391 2089 3732 4304 4589 5366 1391
Size: 698 1643 572 285 777 4072
Fragments arranged by size:
4072 1643 777 698 572 285
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2509 2709 928
Size: 971 610 200 6266
Fragments arranged by size:
6266 971 610 200
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6238 6272 1903
Size: 34 4301 34 3678
Fragments arranged by size:
4301 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5939 7149 5939
Size: 1210 6837
BclI T'GATC_A
Cuts at: 1548 2751 1548
Size: 1203 6844
BfrBI ATG'CAT
Cuts at: 3944 5164 3944
Size: 1220 6827
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3813 4262 6658
Size: 107 122 71 631 605 2141 449 2396
Cuts at: 6658 136
Size: 1525
Fragments arranged by size:
2396 2141 1525 631 605 449 122 107
71
BglII A'GATC_T
Cuts at: 4735 8042 4735
Size: 3307 4740
BlpI GC'TnA_GC
Cuts at: 4442 5464 4442
Size: 1022 7025
Bme1580I G_kGCm'C
Cuts at: 1790 3463 3809 3818 4466 4589 6108 7264 1790
Size: 1673 346 9 648 123 1519 1156 2573
Fragments arranged by size:
2573 1673 1519 1156 648 346 123 9
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8047
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3406 5276 5778
Size: 416 53 83 399 24 1989 1870 502
Cuts at: 5778 6736 442
Size: 958 1753
Fragments arranged by size:
1989 1870 1753 958 502 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8047
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3525 3525
Size: 8047
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2006 3480 3634 4167 4526 4574 4782 6707
Size: 767 1474 154 533 359 48 208 1925
Cuts at: 6707 1239
Size: 2579
Fragments arranged by size:
2579 1925 1474 767 533 359 208 154
48
Bpu10I CC'TnA_GC
Cuts at: 2361 4278 2361
Size: 1917 6130
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3242 6172 7037 7335 3242
Size: 2930 865 298 3954
Fragments arranged by size:
3954 2930 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3822 4803 5089 6710 882
Size: 617 2323 981 286 1621 2219
Fragments arranged by size:
2323 2219 1621 981 617 286
BsaAI yAC'GTr
Cuts at: 493 1204 5838 493
Size: 711 4634 2702
Fragments arranged by size:
4634 2702 711
BsaBI GATnn'nnATC
Cuts at: 8041 8041
Size: 8047
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2069 5854 6236 6871
Size: 53 83 186 936 536 3785 382 635
Cuts at: 6871 275
Size: 1451
Fragments arranged by size:
3785 1451 936 635 536 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4092 6480 6878 7152 7299 7710 4092
Size: 2388 398 274 147 411 4429
Fragments arranged by size:
4429 2388 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2444 2474 3686 3716 5305
Size: 30 165 30 1041 30 1212 30 1589
Cuts at: 5305 5335 1178
Size: 30 3890
Fragments arranged by size:
3890 1589 1212 1041 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2237 2481 2767 3707 4513 1186
Size: 1051 244 286 940 806 4720
Fragments arranged by size:
4720 1051 940 806 286 244
BseYI C'CCAG_C
Cuts at: 3466 4001 4433 7250 7746 3466
Size: 535 432 2817 496 3767
Fragments arranged by size:
3767 2817 535 496 432
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4500 5042 1163
Size: 573 2764 542 4168
Fragments arranged by size:
4168 2764 573 542
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5497 6258 7286 1540
Size: 275 186 3496 761 1028 2301
Fragments arranged by size:
3496 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 6108 6193 7264 6108
Size: 85 1071 6891
Fragments arranged by size:
6891 1071 85
BsmI GAATG_Cn'
Cuts at: 3946 5558 5651 3946
Size: 1612 93 6342
Fragments arranged by size:
6342 1612 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2089 3463 3732 3809 3818 4304 4466
Size: 399 299 1374 269 77 9 486 162
Cuts at: 4466 4589 5366 6108 6193 7264 1391
Size: 123 777 742 85 1071 2174
Fragments arranged by size:
2174 1374 1071 777 742 486 399 299
269 162 123 85 77 9
BspEI T'CCGG_A
Cuts at: 7710 7710
Size: 8047
BspHI T'CATG_A
Cuts at: 5936 7666 5936
Size: 1730 6317
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4279 5014 5295 5402 844
Size: 3435 735 281 107 3489
Fragments arranged by size:
3489 3435 735 281 107
BsrBI CCG'CTC
Cuts at: 1394 1823 3734 4839 5934 1394
Size: 429 1911 1105 1095 3507
Fragments arranged by size:
3507 1911 1105 1095 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2718 6542 6716 65
Size: 1183 1470 3824 174 1396
Fragments arranged by size:
3824 1470 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4092 4895 6691 6878 1700
Size: 110 2282 803 1796 187 2869
Fragments arranged by size:
2869 2282 1796 803 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8047
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8047
BssSI C'ACGA_G
Cuts at: 4595 5235 6107 7119 4595
Size: 640 872 1012 5523
Fragments arranged by size:
5523 1012 872 640
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4430 794
Size: 3636 4411
BstBI TT'CG_AA
Cuts at: 2818 6851 2818
Size: 4033 4014
BstEII G'GTnAC_C
Cuts at: 1184 3635 1184
Size: 2451 5596
BstYI r'GATC_y
Cuts at: 1972 2345 2590 4735 5377 5744 6129 6146 7598
Size: 373 245 2145 642 367 385 17 1452
Cuts at: 7598 7684 7696 8042 1972
Size: 86 12 346 1977
Fragments arranged by size:
2145 1977 1452 642 385 373 367 346
245 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2091 2091
Size: 8047
BtgI C'CryG_G
Cuts at: 513 1065 2004 2061 4474 513
Size: 552 939 57 2413 4086
Fragments arranged by size:
4086 2413 939 552 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7308
Fragments arranged by size:
7308 683 56
BtsI GCAGTG_nn'
Cuts at: 2299 4906 4925 5563 6357 6377 2299
Size: 2607 19 638 794 20 3969
Fragments arranged by size:
3969 2607 794 638 20 19
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8047
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8012
DraI TTT'AAA
Cuts at: 3648 4309 5351 3648
Size: 661 1042 6344
Fragments arranged by size:
6344 1042 661
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4765 7054 809
Size: 507 3449 2289 1802
Fragments arranged by size:
3449 2289 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3429 3785 4834
Size: 54 1425 50 275 18 1599 356 1049
Cuts at: 4834 4929 5494 6383 8012 8
Size: 95 565 889 1629 43
Fragments arranged by size:
1629 1599 1425 1049 889 565 356 275
95 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5494 1537
Size: 275 3682 4090
Fragments arranged by size:
4090 3682 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2036 2329 3041 3994 5977 1353
Size: 683 293 712 953 1983 3423
Fragments arranged by size:
3423 1983 953 712 683 293
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6634 7008 7154 387
Size: 1064 138 5045 374 146 1280
Fragments arranged by size:
5045 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2006 2910 3065 3480 3634 4167 4526
Size: 393 374 904 155 415 154 533 359
Cuts at: 4526 4574 4630 4674 4707 4782 4934 5001 5093
Size: 48 56 44 33 75 152 67 92
Cuts at: 5093 6110 6707 7494 7762 1239
Size: 1017 597 787 268 1524
Fragments arranged by size:
1524 1017 904 787 597 533 415 393
374 359 268 155 154 152 92 75
67 56 48 44 33
EcoNI CCTnn'n_nnAGG
Cuts at: 2094 2094
Size: 8047
EcoO109I rG'GnC_Cy
Cuts at: 2099 2633 3349 4282 4544 4585 4586 4767 2099
Size: 534 716 933 262 41 1 181 5379
Fragments arranged by size:
5379 933 716 534 262 181 41 1
EcoRI G'AATT_C
Cuts at: 3146 5356 5734 3146
Size: 2210 378 5459
Fragments arranged by size:
5459 2210 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2415 4660 2415
Size: 2245 5802
FspI TGC'GCA
Cuts at: 6553 6553
Size: 8047
HaeII r_GCGC'y
Cuts at: 1678 2809 3253 4692 7194 1678
Size: 1131 444 1439 2502 2531
Fragments arranged by size:
2531 2502 1439 1131 444
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3121 3144 3153 3176 3607
Size: 32 135 32 1549 23 9 23 431
Cuts at: 3607 3639 6688 6720 6762 6794 1373
Size: 32 3049 32 42 32 2626
Fragments arranged by size:
3049 2626 1549 431 135 42 32 32
32 32 32 23 23 9
HincII GTy'rAC
Cuts at: 669 1657 1938 2410 2865 3330 5298 5391 5637
Size: 988 281 472 455 465 1968 93 246
Cuts at: 5637 669
Size: 3079
Fragments arranged by size:
3079 1968 988 472 465 455 281 246
93
HindIII A'AGCT_T
Cuts at: 748 4171 5132 748
Size: 3423 961 3663
Fragments arranged by size:
3663 3423 961
HpaI GTT'AAC
Cuts at: 3330 5637 3330
Size: 2307 5740
KpnI G_GTAC'C
Cuts at: 5372 5372
Size: 8047
MfeI C'AATT_G
Cuts at: 3054 5646 3054
Size: 2592 5455
MluI A'CGCG_T
Cuts at: 7923 7923
Size: 8047
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5304 7161 7345 1305
Size: 3999 1857 184 2007
Fragments arranged by size:
3999 2007 1857 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3431 10
Size: 54 1425 343 1599 4626
Fragments arranged by size:
4626 1599 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3365 3518 3716 4749 4868 6005 6364
Size: 648 2199 153 198 1033 119 1137 359
Cuts at: 6364 6523 518
Size: 159 2042
Fragments arranged by size:
2199 2042 1137 1033 648 359 198 159
153 119
MspA1I CmG'CkG
Cuts at: 1606 2052 2063 2811 3466 3607 4005 4582 5463
Size: 446 11 748 655 141 398 577 881
Cuts at: 5463 6140 7288 7533 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 881 748 677 655 577 446
398 245 141 11
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6556
Fragments arranged by size:
6556 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8047
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8047
NotI GC'GGCC_GC
Cuts at: 5494 5494
Size: 8047
NsiI A_TGCA'T
Cuts at: 3946 5166 3946
Size: 1220 6827
NspI r_CATG'y
Cuts at: 1132 1229 2416 3427 4873 5405 6950 1132
Size: 97 1187 1011 1446 532 1545 2229
Fragments arranged by size:
2229 1545 1446 1187 1011 532 97
PasI CC'CwG_GG
Cuts at: 1348 2077 4693 1348
Size: 729 2616 4702
Fragments arranged by size:
4702 2616 729
PciI A'CATG_T
Cuts at: 2412 6946 2412
Size: 4534 3513
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8047
PfoI T'CCnGG_A
Cuts at: 2991 3926 4128 4962 2991
Size: 935 202 834 6076
Fragments arranged by size:
6076 935 834 202
PmeI GTTT'AAAC
Cuts at: 5351 5351
Size: 8047
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6116 6148 7629 7661 6116
Size: 32 1481 32 6502
Fragments arranged by size:
6502 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2099 2633 3349 4282 4544 4767 2099
Size: 534 716 933 262 223 5379
Fragments arranged by size:
5379 933 716 534 262 223
PshAI GACnn'nnGTC
Cuts at: 1613 3834 1613
Size: 2221 5826
PsiI TTA'TAA
Cuts at: 5617 5617
Size: 8047
PspOMI G'GGCC_C
Cuts at: 4585 4585
Size: 8047
PstI C_TGCA'G
Cuts at: 830 5399 830
Size: 4569 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8047
PvuII CAG'CTG
Cuts at: 3466 3607 4005 3466
Size: 141 398 7508
Fragments arranged by size:
7508 398 141
SacII CC_GC'GG
Cuts at: 2064 2064
Size: 8047
SalI G'TCGA_C
Cuts at: 1936 5389 1936
Size: 3453 4594
SanDI GG'GwC_CC
Cuts at: 3349 3349
Size: 8047
SapI GCTCTTCn'nnn_
Cuts at: 2036 3994 2036
Size: 1958 6089
SbfI CC_TGCA'GG
Cuts at: 5399 5399
Size: 8047
ScaI AGT'ACT
Cuts at: 5314 6295 8007 5314
Size: 981 1712 5354
Fragments arranged by size:
5354 1712 981
SfcI C'TryA_G
Cuts at: 826 1045 2899 3076 5180 5395 6530 7211 7402
Size: 219 1854 177 2104 215 1135 681 191
Cuts at: 7402 826
Size: 1471
Fragments arranged by size:
2104 1854 1471 1135 681 219 215 191
177
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8047
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8047
SmaI CCC'GGG
Cuts at: 1872 5374 1872
Size: 3502 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3257 6151 7052 7314 820
Size: 197 926 1314 2894 901 262 1553
Fragments arranged by size:
2894 1553 1314 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5838 493
Size: 5345 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8047
SphI G_CATG'C
Cuts at: 1132 5405 1132
Size: 4273 3774
SspI AAT'ATT
Cuts at: 5 52 5971 5
Size: 47 5919 2081
Fragments arranged by size:
5919 2081 47
StuI AGG'CCT
Cuts at: 1517 4235 1517
Size: 2718 5329
StyI C'CwwG_G
Cuts at: 513 1065 2004 3182 3672 4248 4770 4793 5486
Size: 552 939 1178 490 576 522 23 693
Cuts at: 5486 513
Size: 3074
Fragments arranged by size:
3074 1178 939 693 576 552 522 490
23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3458 4829 6074 6091 6244 6429 6874
Size: 1255 1878 1371 1245 17 153 185 445
Cuts at: 6874 325
Size: 1498
Fragments arranged by size:
1878 1498 1371 1255 1245 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2385 2549 2577
Size: 274 80 33 51 1443 408 164 28
Cuts at: 2577 5312 6293 8005 96
Size: 2735 981 1712 138
Fragments arranged by size:
2735 1712 1443 981 408 274 164 138
80 51 33 28
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4885 4917 8007 8039 4885
Size: 32 3090 32 4893
Fragments arranged by size:
4893 3090 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3542 4937 1316
Size: 2226 1395 4426
Fragments arranged by size:
4426 2226 1395
XbaI T'CTAG_A
Cuts at: 5383 5383
Size: 8047
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2336 3647 4586 4735 2336
Size: 1311 939 149 5648
Fragments arranged by size:
5648 1311 939 149
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8047
XmaI C'CCGG_G
Cuts at: 1870 5372 1870
Size: 3502 4545
XmnI GAAnn'nnTTC
Cuts at: 2030 2370 6176 2030
Size: 340 3806 3901
Fragments arranged by size:
3901 3806 340
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5855 276
Size: 53 83 186 936 4321 2468
Fragments arranged by size:
4321 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacII SalI SanDI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BsiWI
BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI
PsrI RsrII SacI SexAI SfiI SfoI SrfI SwaI