Restriction map for
pFN21AA1430
(Circular) (Six-base) MAPSORT of: pFN21AA1430.seq Check: 1795 from: 1 to: 6301
pFN21AA1430 6301 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4111 278
Size: 53 83 186 936 2575 2468
Fragments arranged by size:
2575 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3644 1937
Size: 1707 4594
Acc65I G'GTAC_C
Cuts at: 3622 3622
Size: 6301
AclI AA'CG_TT
Cuts at: 1563 2475 3373 4428 4801 1563
Size: 912 898 1055 373 3063
Fragments arranged by size:
3063 1055 912 898 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2532 2769 2786 2961 3096 4364 5748 6016
Size: 900 237 17 175 135 1268 1384 268
Cuts at: 6016 1632
Size: 1917
Fragments arranged by size:
1917 1384 1268 900 268 237 175 135
17
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6104
AflIII A'CryG_T
Cuts at: 1203 2341 2343 2632 5200 6177 1203
Size: 1138 2 289 2568 977 1327
Fragments arranged by size:
2568 1327 1138 977 289 2
AgeI A'CCGG_T
Cuts at: 5132 5132
Size: 6301
AhdI GACnn_n'nnGTC
Cuts at: 5030 5030
Size: 6301
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6301
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6301
AlwNI CAG_nnn'CTG
Cuts at: 2582 4044 5616 2582
Size: 1462 1572 3267
Fragments arranged by size:
3267 1572 1462
ApaLI G'TGCA_C
Cuts at: 4358 5514 4358
Size: 1156 5145
ApoI r'AATT_y
Cuts at: 1493 2158 2225 2361 3610 3836 3988 6097 1493
Size: 665 67 136 1249 226 152 2109 1697
Fragments arranged by size:
2109 1697 1249 665 226 152 136 67
AseI AT'TA_AT
Cuts at: 160 1032 4855 160
Size: 872 3823 1606
Fragments arranged by size:
3823 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6301
AvaI C'yCGr_G
Cuts at: 1870 1943 3122 3626 1870
Size: 73 1179 504 4545
Fragments arranged by size:
4545 1179 504 73
BamHI G'GATC_C
Cuts at: 3631 3998 3631
Size: 367 5934
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3622 5077 618
Size: 325 217 627 1835 1455 1842
Fragments arranged by size:
1842 1835 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3620 1391
Size: 2229 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2967 3571 928
Size: 971 1068 604 3658
Fragments arranged by size:
3658 1068 971 604
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4492 4526 1903
Size: 34 2555 34 3678
Fragments arranged by size:
3678 2555 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4193 5403 4193
Size: 1210 5091
BclI T'GATC_A
Cuts at: 1548 2830 3103 3250 1548
Size: 1282 273 147 4599
Fragments arranged by size:
4599 1282 273 147
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4912 136
Size: 107 122 71 631 605 3240 1525
Fragments arranged by size:
3240 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6296 6296
Size: 6301
BlpI GC'TnA_GC
Cuts at: 3718 3718
Size: 6301
Bme1580I G_kGCm'C
Cuts at: 1790 4362 5518 1790
Size: 2572 1156 2573
Fragments arranged by size:
2573 2572 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6301
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2825 4032 4990
Size: 416 53 83 399 24 1408 1207 958
Cuts at: 4990 442
Size: 1753
Fragments arranged by size:
1753 1408 1207 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6301
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2769 2974 3529 4961 1239
Size: 1530 205 555 1432 2579
Fragments arranged by size:
2579 1530 1432 555 205
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3362 4426 5291 5589 3362
Size: 1064 865 298 4074
Fragments arranged by size:
4074 1064 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3512 4964 882
Size: 617 2013 1452 2219
Fragments arranged by size:
2219 2013 1452 617
BsaAI yAC'GTr
Cuts at: 493 1204 2344 4092 493
Size: 711 1140 1748 2702
Fragments arranged by size:
2702 1748 1140 711
BsaBI GATnn'nnATC
Cuts at: 2589 3042 6295 2589
Size: 453 3253 2595
Fragments arranged by size:
3253 2595 453
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4108 4490 5125 275
Size: 53 83 186 936 2575 382 635 1451
Fragments arranged by size:
2575 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4734 5132 5406 5553 5964 4734
Size: 398 274 147 411 5071
Fragments arranged by size:
5071 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6076
Fragments arranged by size:
6076 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2506 2515 2518 2756 2953 1186
Size: 1320 9 3 238 197 4534
Fragments arranged by size:
4534 1320 238 197 9 3
BseYI C'CCAG_C
Cuts at: 5504 6000 5504
Size: 496 5805
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5728
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3169 3751 4512 5540 1540
Size: 275 186 1168 582 761 1028 2301
Fragments arranged by size:
2301 1168 1028 761 582 275 186
BsiHKAI G_wGCw'C
Cuts at: 3280 4362 4447 5518 3280
Size: 1082 85 1071 4063
Fragments arranged by size:
4063 1082 1071 85
BsmI GAATG_Cn'
Cuts at: 2178 3812 3905 2178
Size: 1634 93 4574
Fragments arranged by size:
4574 1634 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2610 2610
Size: 6301
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3280 3620 4362 4447 5518 1391
Size: 399 1490 340 742 85 1071 2174
Fragments arranged by size:
2174 1490 1071 742 399 340 85
BspEI T'CCGG_A
Cuts at: 5964 5964
Size: 6301
BspHI T'CATG_A
Cuts at: 4190 5920 4190
Size: 1730 4571
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3656 844
Size: 2812 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4188 1394
Size: 429 2365 3507
Fragments arranged by size:
3507 2365 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2282 4796 4970 65
Size: 1183 1034 2514 174 1396
Fragments arranged by size:
2514 1396 1183 1034 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4945 5132 1700
Size: 110 3135 187 2869
Fragments arranged by size:
3135 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6301
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6301
BssSI C'ACGA_G
Cuts at: 4361 5373 4361
Size: 1012 5289
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6301
BstBI TT'CG_AA
Cuts at: 2162 5105 2162
Size: 2943 3358
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6301
BstYI r'GATC_y
Cuts at: 1972 3037 3446 3631 3998 4383 4400 5852 5938
Size: 1065 409 185 367 385 17 1452 86
Cuts at: 5938 5950 6296 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1065 409 385 367 346 185
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2739 2739
Size: 6301
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4810
Fragments arranged by size:
4810 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3383 487
Size: 683 56 2157 3405
Fragments arranged by size:
3405 2157 683 56
BtsI GCAGTG_nn'
Cuts at: 2425 3817 4611 4631 2425
Size: 1392 794 20 4095
Fragments arranged by size:
4095 1392 794 20
ClaI AT'CG_AT
Cuts at: 1554 3165 1554
Size: 1611 4690
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6266
DraI TTT'AAA
Cuts at: 3605 3605
Size: 6301
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5308 809
Size: 507 3992 1802
Fragments arranged by size:
3992 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2728 3457 3550
Size: 54 1425 50 275 18 898 729 93
Cuts at: 3550 3748 4637 6266 8
Size: 198 889 1629 43
Fragments arranged by size:
1629 1425 898 889 729 275 198 93
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3748 1537
Size: 275 1936 4090
Fragments arranged by size:
4090 1936 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2299 2524 2805 3144 4231 1353
Size: 946 225 281 339 1087 3423
Fragments arranged by size:
3423 1087 946 339 281 225
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3224 4888 5262 5408 387
Size: 1064 138 1635 1664 374 146 1280
Fragments arranged by size:
1664 1635 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2532 2769 2786 2961 2974 3096 3529
Size: 393 900 237 17 175 13 122 433
Cuts at: 3529 4364 4961 5748 6016 1239
Size: 835 597 787 268 1524
Fragments arranged by size:
1524 900 835 787 597 433 393 268
237 175 122 17 13
EcoRI G'AATT_C
Cuts at: 2361 3610 3988 2361
Size: 1249 378 4674
Fragments arranged by size:
4674 1249 378
FspI TGC'GCA
Cuts at: 4807 4807
Size: 6301
HaeII r_GCGC'y
Cuts at: 1678 5448 1678
Size: 3770 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2564 2596 2948 2980 3379
Size: 32 135 32 992 32 352 32 399
Cuts at: 3379 3411 4942 4974 5016 5048 1373
Size: 32 1531 32 42 32 2626
Fragments arranged by size:
2626 1531 992 399 352 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3538 3645 3891 669
Size: 988 281 1600 107 246 3079
Fragments arranged by size:
3079 1600 988 281 246 107
HindIII A'AGCT_T
Cuts at: 748 3057 748
Size: 2309 3992
HpaI GTT'AAC
Cuts at: 3891 3891
Size: 6301
KpnI G_GTAC'C
Cuts at: 3626 3626
Size: 6301
MfeI C'AATT_G
Cuts at: 3900 3900
Size: 6301
MluI A'CGCG_T
Cuts at: 6177 6177
Size: 6301
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5415 5599 1305
Size: 4110 184 2007
Fragments arranged by size:
4110 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2730 3459 3552 10
Size: 54 1425 343 898 729 93 2759
Fragments arranged by size:
2759 1425 898 729 343 93 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2171 2722 4259 4618 4777 518
Size: 648 1005 551 1537 359 159 2042
Fragments arranged by size:
2042 1537 1005 648 551 359 159
MspA1I CmG'CkG
Cuts at: 1606 3533 3717 4394 5542 5787 1606
Size: 1927 184 677 1148 245 2120
Fragments arranged by size:
2120 1927 1148 677 245 184
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4810
Fragments arranged by size:
4810 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6301
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6301
NotI GC'GGCC_GC
Cuts at: 3748 3748
Size: 6301
NspI r_CATG'y
Cuts at: 1132 1229 2636 3659 5204 1132
Size: 97 1407 1023 1545 2229
Fragments arranged by size:
2229 1545 1407 1023 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6301
PciI A'CATG_T
Cuts at: 2632 5200 2632
Size: 2568 3733
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2726 3362 1108
Size: 1618 636 4047
Fragments arranged by size:
4047 1618 636
PfoI T'CCnGG_A
Cuts at: 3482 3482
Size: 6301
PmeI GTTT'AAAC
Cuts at: 3605 3605
Size: 6301
PmlI CAC'GTG
Cuts at: 2344 2344
Size: 6301
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4370 4402 5883 5915 4370
Size: 32 1481 32 4756
Fragments arranged by size:
4756 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2769 1613
Size: 1156 5145
PsiI TTA'TAA
Cuts at: 3871 3871
Size: 6301
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3358 3390 3358
Size: 32 6269
PstI C_TGCA'G
Cuts at: 830 3653 830
Size: 2823 3478
PvuI CG_AT'CG
Cuts at: 2001 3169 2001
Size: 1168 5133
SalI G'TCGA_C
Cuts at: 1936 3643 1936
Size: 1707 4594
SbfI CC_TGCA'GG
Cuts at: 3653 3653
Size: 6301
ScaI AGT'ACT
Cuts at: 2713 4549 6261 2713
Size: 1836 1712 2753
Fragments arranged by size:
2753 1836 1712
SfcI C'TryA_G
Cuts at: 826 1045 3649 4784 5465 5656 826
Size: 219 2604 1135 681 191 1471
Fragments arranged by size:
2604 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6301
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6301
SmaI CCC'GGG
Cuts at: 1872 3124 3628 1872
Size: 1252 504 4545
Fragments arranged by size:
4545 1252 504
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3341 4405 5306 5568 820
Size: 197 926 1398 1064 901 262 1553
Fragments arranged by size:
1553 1398 1064 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4092 493
Size: 3599 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6301
SphI G_CATG'C
Cuts at: 1132 3659 1132
Size: 2527 3774
SspI AAT'ATT
Cuts at: 5 52 2915 4225 5
Size: 47 2863 1310 2081
Fragments arranged by size:
2863 2081 1310 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6301
StyI C'CwwG_G
Cuts at: 513 1065 2004 3740 513
Size: 552 939 1736 3074
Fragments arranged by size:
3074 1736 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4328 4345 4498 4683 5128 325
Size: 1255 2748 17 153 185 445 1498
Fragments arranged by size:
2748 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2711 3230 4547
Size: 274 80 33 51 1443 734 519 1317
Cuts at: 4547 6259 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1317 734 519 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3478 3510 6261 6293 3478
Size: 32 2751 32 3486
Fragments arranged by size:
3486 2751 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6301
XbaI T'CTAG_A
Cuts at: 3637 3637
Size: 6301
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6301
XmaI C'CCGG_G
Cuts at: 1870 3122 3626 1870
Size: 1252 504 4545
Fragments arranged by size:
4545 1252 504
XmnI GAAnn'nnTTC
Cuts at: 4430 4430
Size: 6301
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4109 276
Size: 53 83 186 936 2575 2468
Fragments arranged by size:
2575 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PsrI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI
PspXI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI