Restriction map for
pFN21AA1429
(Circular) (Six-base) MAPSORT of: pFN21AA1429.seq Check: 2641 from: 1 to: 10096
pFN21AA1429 10096 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7906 278
Size: 53 83 186 936 6370 2468
Fragments arranged by size:
6370 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2817 3300 5657 6901 6946 7439 1937
Size: 880 483 2357 1244 45 493 4594
Fragments arranged by size:
4594 2357 1244 880 493 483 45
Acc65I G'GTAC_C
Cuts at: 7417 7417
Size: 10096
AclI AA'CG_TT
Cuts at: 1563 8223 8596 1563
Size: 6660 373 3063
Fragments arranged by size:
6660 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2982 3006 3040 4923 5091 5343 5619 5934
Size: 1350 24 34 1883 168 252 276 315
Cuts at: 5934 6512 6639 8159 9543 9811 1632
Size: 578 127 1520 1384 268 1917
Fragments arranged by size:
1917 1883 1520 1384 1350 578 315 276
268 252 168 127 34 24
AfeI AGC'GCT
Cuts at: 2062 3336 5112 6716 2062
Size: 1274 1776 1604 5442
Fragments arranged by size:
5442 1776 1604 1274
AflII C'TTAA_G
Cuts at: 820 1017 3500 6443 820
Size: 197 2483 2943 4473
Fragments arranged by size:
4473 2943 2483 197
AflIII A'CryG_T
Cuts at: 1203 4807 4914 6041 7393 8995 9972 1203
Size: 3604 107 1127 1352 1602 977 1327
Fragments arranged by size:
3604 1602 1352 1327 1127 977 107
AgeI A'CCGG_T
Cuts at: 8927 8927
Size: 10096
AhdI GACnn_n'nnGTC
Cuts at: 8825 8825
Size: 10096
AleI CACnn'nnGTG
Cuts at: 1166 4098 1166
Size: 2932 7164
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 10096
AlwNI CAG_nnn'CTG
Cuts at: 2615 3779 6454 6535 7839 9411 2615
Size: 1164 2675 81 1304 1572 3300
Fragments arranged by size:
3300 2675 1572 1304 1164 81
ApaLI G'TGCA_C
Cuts at: 8153 9309 8153
Size: 1156 8940
ApoI r'AATT_y
Cuts at: 1493 2680 2904 3127 3222 3322 4189 5181 6200
Size: 1187 224 223 95 100 867 992 1019
Cuts at: 6200 6213 6355 7405 7631 7783 9892 1493
Size: 13 142 1050 226 152 2109 1697
Fragments arranged by size:
2109 1697 1187 1050 1019 992 867 226
224 223 152 142 100 95 13
AseI AT'TA_AT
Cuts at: 160 1032 5742 6303 8650 160
Size: 872 4710 561 2347 1606
Fragments arranged by size:
4710 2347 1606 872 561
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10096
AvaI C'yCGr_G
Cuts at: 1870 1943 2056 7091 7193 7421 1870
Size: 73 113 5035 102 228 4545
Fragments arranged by size:
5035 4545 228 113 102 73
BamHI G'GATC_C
Cuts at: 5075 7426 7793 5075
Size: 2351 367 7378
Fragments arranged by size:
7378 2351 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 7417 8872 618
Size: 325 217 627 5630 1455 1842
Fragments arranged by size:
5630 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2152 2664 3418 4207 6280 6850 7415 1391
Size: 761 512 754 789 2073 570 565 4072
Fragments arranged by size:
4072 2073 789 761 754 570 565 512
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2994 3737 4148 4578 5625 928
Size: 971 1095 743 411 430 1047 5399
Fragments arranged by size:
5399 1095 1047 971 743 430 411
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3758 3792 8287 8321 1903
Size: 34 1821 34 4495 34 3678
Fragments arranged by size:
4495 3678 1821 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2967 7988 9198 2967
Size: 5021 1210 3865
Fragments arranged by size:
5021 3865 1210
BclI T'GATC_A
Cuts at: 1548 3450 3481 3763 4222 5878 6580 1548
Size: 1902 31 282 459 1656 702 5064
Fragments arranged by size:
5064 1902 1656 702 459 282 31
BfrBI ATG'CAT
Cuts at: 4271 4560 6961 4271
Size: 289 2401 7406
Fragments arranged by size:
7406 2401 289
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4966 8707 136
Size: 107 122 71 631 605 3294 3741 1525
Fragments arranged by size:
3741 3294 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4375 4861 6634 10091 4375
Size: 486 1773 3457 4380
Fragments arranged by size:
4380 3457 1773 486
BlpI GC'TnA_GC
Cuts at: 2064 7513 2064
Size: 5449 4647
Bme1580I G_kGCm'C
Cuts at: 1790 2248 8157 9313 1790
Size: 458 5909 1156 2573
Fragments arranged by size:
5909 2573 1156 458
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 10096
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 7827 8785 442
Size: 416 53 83 399 24 6410 958 1753
Fragments arranged by size:
6410 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 6008 1056
Size: 4952 5144
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5959 5959
Size: 10096
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2544 3975 4478 5835 5954 6476 6501 6726
Size: 1305 1431 503 1357 119 522 25 225
Cuts at: 6726 7050 8756 1239
Size: 324 1706 2579
Fragments arranged by size:
2579 1706 1431 1357 1305 522 503 324
225 119 25
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3402 3806 6990 7251 8221 9086 9384 3402
Size: 404 3184 261 970 865 298 4114
Fragments arranged by size:
4114 3184 970 865 404 298 261
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4247 5814 6263 6788 8759 882
Size: 617 2748 1567 449 525 1971 2219
Fragments arranged by size:
2748 2219 1971 1567 617 525 449
BsaAI yAC'GTr
Cuts at: 493 1204 3487 4808 7887 493
Size: 711 2283 1321 3079 2702
Fragments arranged by size:
3079 2702 2283 1321 711
BsaBI GATnn'nnATC
Cuts at: 3837 6408 6633 7332 10090 3837
Size: 2571 225 699 2758 3843
Fragments arranged by size:
3843 2758 2571 699 225
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 7903 8285 8920 275
Size: 53 83 186 936 6370 382 635 1451
Fragments arranged by size:
6370 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 8529 8927 9201 9348 9759 8529
Size: 398 274 147 411 8866
Fragments arranged by size:
8866 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3973 4003 5115 5145 5331
Size: 30 165 30 2570 30 1112 30 186
Cuts at: 5331 5361 5808 5838 1178
Size: 30 447 30 5436
Fragments arranged by size:
5436 2570 1112 447 186 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2547 2628 2846 4247 7079 1186
Size: 1361 81 218 1401 2832 4203
Fragments arranged by size:
4203 2832 1401 1361 218 81
BseYI C'CCAG_C
Cuts at: 2451 3377 9299 9795 2451
Size: 926 5922 496 2752
Fragments arranged by size:
5922 2752 926 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4376 5011 5562 1163
Size: 573 2640 635 551 5697
Fragments arranged by size:
5697 2640 635 573 551
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7546 8307 9335 1540
Size: 275 186 5545 761 1028 2301
Fragments arranged by size:
5545 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4093 4207 5141 6127 6280 6850 7080 8157 8242
Size: 114 934 986 153 570 230 1077 85
Cuts at: 8242 9313 4093
Size: 1071 4876
Fragments arranged by size:
4876 1077 1071 986 934 570 230 153
114 85
BsmI GAATG_Cn'
Cuts at: 7607 7700 7607
Size: 93 10003
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2152 2248 2664 3418 4093 4207 5141
Size: 399 362 96 416 754 675 114 934
Cuts at: 5141 6127 6280 6850 7080 7415 8157 8242 9313
Size: 986 153 570 230 335 742 85 1071
Cuts at: 9313 1391
Size: 2174
Fragments arranged by size:
2174 1071 986 934 754 742 675 570
416 399 362 335 230 153 114 96
85
BspEI T'CCGG_A
Cuts at: 9759 9759
Size: 10096
BspHI T'CATG_A
Cuts at: 2404 6910 7985 9715 2404
Size: 4506 1075 1730 2785
Fragments arranged by size:
4506 2785 1730 1075
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5519 6844 7451 844
Size: 4675 1325 607 3489
Fragments arranged by size:
4675 3489 1325 607
BsrBI CCG'CTC
Cuts at: 1394 1823 7983 1394
Size: 429 6160 3507
Fragments arranged by size:
6160 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4304 4316 5372 5886 8591 8765 65
Size: 1183 3056 12 1056 514 2705 174 1396
Fragments arranged by size:
3056 2705 1396 1183 1056 514 174 12
BsrFI r'CCGG_y
Cuts at: 1700 1810 8740 8927 1700
Size: 110 6930 187 2869
Fragments arranged by size:
6930 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4346 96
Size: 4250 5846
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10096
BssSI C'ACGA_G
Cuts at: 7372 8156 9168 7372
Size: 784 1012 8300
Fragments arranged by size:
8300 1012 784
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5511 794
Size: 4717 5379
BstBI TT'CG_AA
Cuts at: 8900 8900
Size: 10096
BstEII G'GTnAC_C
Cuts at: 1184 3713 4057 6467 7177 1184
Size: 2529 344 2410 710 4103
Fragments arranged by size:
4103 2529 2410 710 344
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 4253 2011
Size: 2242 7854
BstYI r'GATC_y
Cuts at: 1972 3838 4375 4861 5075 5788 6634 7426 7793
Size: 1866 537 486 214 713 846 792 367
Cuts at: 7793 8178 8195 9647 9733 9745 10091 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1866 1452 846 792 713 537 486
385 367 346 214 86 17 12
BstZ17I GTA'TAC
Cuts at: 5658 6902 5658
Size: 1244 8852
Bsu36I CC'TnA_GG
Cuts at: 2829 2829
Size: 10096
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 8605
Fragments arranged by size:
8605 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2036 487
Size: 683 56 810 8547
Fragments arranged by size:
8547 810 683 56
BtsI GCAGTG_nn'
Cuts at: 2979 4797 7263 7299 7612 8406 8426 2979
Size: 1818 2466 36 313 794 20 4649
Fragments arranged by size:
4649 2466 1818 794 313 36 20
ClaI AT'CG_AT
Cuts at: 1554 4761 1554
Size: 3207 6889
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 10061
DraI TTT'AAA
Cuts at: 2050 3321 3517 6096 7400 2050
Size: 1271 196 2579 1304 4746
Fragments arranged by size:
4746 2579 1304 1271 196
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 9103 809
Size: 507 7787 1802
Fragments arranged by size:
7787 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3289 4441 5262
Size: 54 1425 50 275 18 1459 1152 821
Cuts at: 5262 7031 7543 8432 10061 8
Size: 1769 512 889 1629 43
Fragments arranged by size:
1769 1629 1459 1425 1152 889 821 512
275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7543 1537
Size: 275 5731 4090
Fragments arranged by size:
5731 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2622 2721 2778 2832 2844 2859 3747 5043
Size: 1269 99 57 54 12 15 888 1296
Cuts at: 5043 5247 5373 5691 5914 6531 6615 7151 8026
Size: 204 126 318 223 617 84 536 875
Cuts at: 8026 1353
Size: 3423
Fragments arranged by size:
3423 1296 1269 888 875 617 536 318
223 204 126 99 84 57 54 15
12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 8683 9057 9203 387
Size: 1064 138 7094 374 146 1280
Fragments arranged by size:
7094 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4205 6278 6848 4205
Size: 2073 570 7453
Fragments arranged by size:
7453 2073 570
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2544 2982 3006 3040 3975 4478 4923
Size: 393 912 438 24 34 935 503 445
Cuts at: 4923 5091 5343 5619 5835 5934 5954 6476 6501
Size: 168 252 276 216 99 20 522 25
Cuts at: 6501 6512 6639 6726 7050 8159 8756 9543 9811
Size: 11 127 87 324 1109 597 787 268
Cuts at: 9811 1239
Size: 1524
Fragments arranged by size:
1524 1109 935 912 787 597 522 503
445 438 393 324 276 268 252 216
168 127 99 87 34 25 24 20
11
EcoNI CCTnn'n_nnAGG
Cuts at: 4845 4845
Size: 10096
EcoO109I rG'GnC_Cy
Cuts at: 2564 7141 2564
Size: 4577 5519
EcoRI G'AATT_C
Cuts at: 2680 7405 7783 2680
Size: 4725 378 4993
Fragments arranged by size:
4993 4725 378
EcoRV GAT'ATC
Cuts at: 4028 5194 4028
Size: 1166 8930
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3302 3353 5315 6673 3302
Size: 51 1962 1358 6725
Fragments arranged by size:
6725 1962 1358 51
FspI TGC'GCA
Cuts at: 8602 8602
Size: 10096
HaeII r_GCGC'y
Cuts at: 1678 2064 3338 5114 6718 9243 1678
Size: 386 1274 1776 1604 2525 2531
Fragments arranged by size:
2531 2525 1776 1604 1274 386
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2399 2431 3004 3036 3473
Size: 32 135 32 827 32 573 32 437
Cuts at: 3473 3505 3724 3756 3988 4020 4189 4221 4511
Size: 32 219 32 232 32 169 32 290
Cuts at: 4511 4543 5331 5363 5552 5584 6170 6202 6686
Size: 32 788 32 189 32 586 32 484
Cuts at: 6686 6718 8737 8769 8811 8843 1373
Size: 32 2019 32 42 32 2626
Fragments arranged by size:
2626 2019 827 788 586 573 484 437
290 232 219 189 169 135 42 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3159 4853 4978 5055 6655 7440
Size: 988 281 1221 1694 125 77 1600 785
Cuts at: 7440 7686 669
Size: 246 3079
Fragments arranged by size:
3079 1694 1600 1221 988 785 281 246
125 77
HindIII A'AGCT_T
Cuts at: 748 3315 3519 3558 3602 3822 4634 5712 6377
Size: 2567 204 39 44 220 812 1078 665
Cuts at: 6377 748
Size: 4467
Fragments arranged by size:
4467 2567 1078 812 665 220 204 44
39
HpaI GTT'AAC
Cuts at: 3159 5055 7686 3159
Size: 1896 2631 5569
Fragments arranged by size:
5569 2631 1896
KpnI G_GTAC'C
Cuts at: 7421 7421
Size: 10096
MfeI C'AATT_G
Cuts at: 3260 5552 6231 7695 3260
Size: 2292 679 1464 5661
Fragments arranged by size:
5661 2292 1464 679
MluI A'CGCG_T
Cuts at: 9972 9972
Size: 10096
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3967 4152 5583 6405 6418 7051 9210 9394
Size: 2662 185 1431 822 13 633 2159 184
Cuts at: 9394 1305
Size: 2007
Fragments arranged by size:
2662 2159 2007 1431 822 633 185 184
13
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3291 4443 5264 10
Size: 54 1425 343 1459 1152 821 4842
Fragments arranged by size:
4842 1459 1425 1152 821 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2082 2490 3539 3681 4098 4218
Size: 648 843 73 408 1049 142 417 120
Cuts at: 4218 4309 5457 6078 6789 6966 8054 8413 8572
Size: 91 1148 621 711 177 1088 359 159
Cuts at: 8572 518
Size: 2042
Fragments arranged by size:
2042 1148 1088 1049 843 711 648 621
417 408 359 177 159 142 120 91
73
MspA1I CmG'CkG
Cuts at: 1606 4436 7512 8189 9337 9582 1606
Size: 2830 3076 677 1148 245 2120
Fragments arranged by size:
3076 2830 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 8605
Fragments arranged by size:
8605 939 552
NdeI CA'TA_TG
Cuts at: 387 2179 3016 3025 5127 5529 387
Size: 1792 837 9 2102 402 4954
Fragments arranged by size:
4954 2102 1792 837 402 9
NheI G'CTAG_C
Cuts at: 1052 6004 1052
Size: 4952 5144
NotI GC'GGCC_GC
Cuts at: 7543 7543
Size: 10096
NsiI A_TGCA'T
Cuts at: 4273 4562 6963 4273
Size: 289 2401 7406
Fragments arranged by size:
7406 2401 289
NspI r_CATG'y
Cuts at: 1132 1229 2489 4560 4619 4830 5098 5452 6045
Size: 97 1260 2071 59 211 268 354 593
Cuts at: 6045 6965 7397 7454 8999 1132
Size: 920 432 57 1545 2229
Fragments arranged by size:
2229 2071 1545 1260 920 593 432 354
268 211 97 59 57
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 10096
PciI A'CATG_T
Cuts at: 6041 7393 8995 6041
Size: 1352 1602 7142
Fragments arranged by size:
7142 1602 1352
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2615 1108
Size: 1507 8589
PfoI T'CCnGG_A
Cuts at: 2560 5784 5812 6742 2560
Size: 3224 28 930 5914
Fragments arranged by size:
5914 3224 930 28
PmeI GTTT'AAAC
Cuts at: 7400 7400
Size: 10096
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8165 8197 9678 9710 8165
Size: 32 1481 32 8551
Fragments arranged by size:
8551 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2564 2564
Size: 10096
PshAI GACnn'nnGTC
Cuts at: 1613 6044 1613
Size: 4431 5665
PsiI TTA'TAA
Cuts at: 4548 6803 7666 4548
Size: 2255 863 6978
Fragments arranged by size:
6978 2255 863
PspXI vC'TCGA_Gb
Cuts at: 7091 7091
Size: 10096
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 6742 6774 6742
Size: 32 10064
PstI C_TGCA'G
Cuts at: 830 6313 7448 830
Size: 5483 1135 3478
Fragments arranged by size:
5483 3478 1135
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10096
PvuII CAG'CTG
Cuts at: 4436 4436
Size: 10096
SacI G_AGCT'C
Cuts at: 4207 6280 6850 4207
Size: 2073 570 7453
Fragments arranged by size:
7453 2073 570
SalI G'TCGA_C
Cuts at: 1936 7438 1936
Size: 5502 4594
SapI GCTCTTCn'nnn_
Cuts at: 5691 5914 5691
Size: 223 9873
SbfI CC_TGCA'GG
Cuts at: 7448 7448
Size: 10096
ScaI AGT'ACT
Cuts at: 4496 6507 8344 10056 4496
Size: 2011 1837 1712 4536
Fragments arranged by size:
4536 2011 1837 1712
SexAI A'CCwGG_T
Cuts at: 5672 5672
Size: 10096
SfcI C'TryA_G
Cuts at: 826 1045 4286 6309 6682 7172 7444 8579 9260
Size: 219 3241 2023 373 490 272 1135 681
Cuts at: 9260 9451 826
Size: 191 1471
Fragments arranged by size:
3241 2023 1471 1135 681 490 373 272
219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10096
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10096
SmaI CCC'GGG
Cuts at: 1872 7423 1872
Size: 5551 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3417 3500 3785 6443 7005 7091
Size: 197 926 1474 83 285 2658 562 86
Cuts at: 7091 7266 8200 9101 9363 820
Size: 175 934 901 262 1553
Fragments arranged by size:
2658 1553 1474 934 926 901 562 285
262 197 175 86 83
SnaBI TAC'GTA
Cuts at: 493 7887 493
Size: 7394 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10096
SphI G_CATG'C
Cuts at: 1132 4560 4830 5452 7454 1132
Size: 3428 270 622 2002 3774
Fragments arranged by size:
3774 3428 2002 622 270
SspI AAT'ATT
Cuts at: 5 52 2705 8020 5
Size: 47 2653 5315 2081
Fragments arranged by size:
5315 2653 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 10096
StyI C'CwwG_G
Cuts at: 513 1065 2004 2540 3286 4512 4961 7535 513
Size: 552 939 536 746 1226 449 2574 3074
Fragments arranged by size:
3074 2574 1226 939 746 552 536 449
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4505 8123 8140 8293 8478 8923 325
Size: 1255 2925 3618 17 153 185 445 1498
Fragments arranged by size:
3618 2925 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4346 4494 6505
Size: 274 80 33 51 1443 2369 148 2011
Cuts at: 6505 8342 10054 96
Size: 1837 1712 138
Fragments arranged by size:
2369 2011 1837 1712 1443 274 148 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 10056 10088 10056
Size: 32 10064
Tth111I GACn'n_nGTC
Cuts at: 1316 3530 1316
Size: 2214 7882
XbaI T'CTAG_A
Cuts at: 7207 7432 7207
Size: 225 9871
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4510 4510
Size: 10096
XhoI C'TCGA_G
Cuts at: 1943 7091 1943
Size: 5148 4948
XmaI C'CCGG_G
Cuts at: 1870 7421 1870
Size: 5551 4545
XmnI GAAnn'nnTTC
Cuts at: 3222 3906 5487 6525 6999 8225 3222
Size: 684 1581 1038 474 1226 5093
Fragments arranged by size:
5093 1581 1226 1038 684 474
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7904 276
Size: 53 83 186 936 6370 2468
Fragments arranged by size:
6370 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI
PsrI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsiWI BsmBI DraIII FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PmlI PspOMI RsrII SacII
SanDI SfiI SfoI SrfI SwaI