Restriction map for
pFN21AA1428
(Circular) (Six-base) MAPSORT of: pFN21AA1428.seq Check: 9213 from: 1 to: 6832
pFN21AA1428 6832 bp
With 182 enzymes: SgfI *
June 30, 2009 15:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4642 278
Size: 53 83 186 936 3106 2468
Fragments arranged by size:
3106 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3308 4027 4175 1937
Size: 1371 719 148 4594
Fragments arranged by size:
4594 1371 719 148
Acc65I G'GTAC_C
Cuts at: 4153 4153
Size: 6832
AclI AA'CG_TT
Cuts at: 1563 4959 5332 1563
Size: 3396 373 3063
Fragments arranged by size:
3396 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2552 3504 4895 6279 6547 1632
Size: 920 952 1391 1384 268 1917
Fragments arranged by size:
1917 1391 1384 952 920 268
AflII C'TTAA_G
Cuts at: 820 1017 3139 820
Size: 197 2122 4513
Fragments arranged by size:
4513 2122 197
AflIII A'CryG_T
Cuts at: 1203 5731 6708 1203
Size: 4528 977 1327
Fragments arranged by size:
4528 1327 977
AgeI A'CCGG_T
Cuts at: 5663 5663
Size: 6832
AhdI GACnn_n'nnGTC
Cuts at: 5561 5561
Size: 6832
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6832
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6832
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2685 2717 2685
Size: 32 6800
AlwNI CAG_nnn'CTG
Cuts at: 4575 6147 4575
Size: 1572 5260
ApaLI G'TGCA_C
Cuts at: 4889 6045 4889
Size: 1156 5676
ApoI r'AATT_y
Cuts at: 1493 2675 2813 2830 2926 3313 4141 4367 4519
Size: 1182 138 17 96 387 828 226 152
Cuts at: 4519 6628 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1182 828 387 226 152 138
96 17
AseI AT'TA_AT
Cuts at: 160 1032 2436 5386 160
Size: 872 1404 2950 1606
Fragments arranged by size:
2950 1606 1404 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6832
AvaI C'yCGr_G
Cuts at: 1870 1943 3597 4157 1870
Size: 73 1654 560 4545
Fragments arranged by size:
4545 1654 560 73
BamHI G'GATC_C
Cuts at: 3290 4162 4529 3290
Size: 872 367 5593
Fragments arranged by size:
5593 872 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4153 5608 618
Size: 325 217 627 2366 1455 1842
Fragments arranged by size:
2366 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2255 4151 1391
Size: 864 1896 4072
Fragments arranged by size:
4072 1896 864
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3018 928
Size: 971 1119 4742
Fragments arranged by size:
4742 1119 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5023 5057 1903
Size: 34 50 34 3002 34 3678
Fragments arranged by size:
3678 3002 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2843 4724 5934 2843
Size: 1881 1210 3741
Fragments arranged by size:
3741 1881 1210
BclI T'GATC_A
Cuts at: 1548 2419 1548
Size: 871 5961
BfrBI ATG'CAT
Cuts at: 2137 2542 2137
Size: 405 6427
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3429 5443 136
Size: 107 122 71 631 605 1757 2014 1525
Fragments arranged by size:
2014 1757 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2368 6827 2368
Size: 4459 2373
BlpI GC'TnA_GC
Cuts at: 2291 4249 2291
Size: 1958 4874
Bme1580I G_kGCm'C
Cuts at: 1790 4893 6049 1790
Size: 3103 1156 2573
Fragments arranged by size:
3103 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6832
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2347 4563 5521
Size: 416 53 83 399 24 930 2216 958
Cuts at: 5521 442
Size: 1753
Fragments arranged by size:
2216 1753 958 930 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6832
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2311 2311
Size: 6832
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2063 3480 5492 1239
Size: 824 1417 2012 2579
Fragments arranged by size:
2579 2012 1417 824
Bpu10I CC'TnA_GC
Cuts at: 4067 4067
Size: 6832
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4957 5822 6120 4957
Size: 865 298 5669
Fragments arranged by size:
5669 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2732 5495 882
Size: 617 1233 2763 2219
Fragments arranged by size:
2763 2219 1233 617
BsaAI yAC'GTr
Cuts at: 493 1204 4623 493
Size: 711 3419 2702
Fragments arranged by size:
3419 2702 711
BsaBI GATnn'nnATC
Cuts at: 3552 6826 3552
Size: 3274 3558
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4639 5021 5656 275
Size: 53 83 186 936 3106 382 635 1451
Fragments arranged by size:
3106 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5265 5663 5937 6084 6495 5265
Size: 398 274 147 411 5602
Fragments arranged by size:
5602 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6607
Fragments arranged by size:
6607 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2048 1186
Size: 862 5970
BseYI C'CCAG_C
Cuts at: 6035 6531 6035
Size: 496 6336
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2190 3150 1163
Size: 573 454 960 4845
Fragments arranged by size:
4845 960 573 454
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4282 5043 6071 1540
Size: 275 186 2281 761 1028 2301
Fragments arranged by size:
2301 2281 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2255 4893 4978 6049 2255
Size: 2638 85 1071 3038
Fragments arranged by size:
3038 2638 1071 85
BsmI GAATG_Cn'
Cuts at: 4343 4436 4343
Size: 93 6739
BsmBI CGTCTCn'nnnn_
Cuts at: 2030 2030
Size: 6832
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2255 4151 4893 4978 6049 1391
Size: 399 465 1896 742 85 1071 2174
Fragments arranged by size:
2174 1896 1071 742 465 399 85
BspEI T'CCGG_A
Cuts at: 6495 6495
Size: 6832
BspHI T'CATG_A
Cuts at: 2422 3091 4721 6451 2422
Size: 669 1630 1730 2803
Fragments arranged by size:
2803 1730 1630 669
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4187 844
Size: 3343 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4719 1394
Size: 429 2896 3507
Fragments arranged by size:
3507 2896 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2258 5327 5501 65
Size: 1183 1010 3069 174 1396
Fragments arranged by size:
3069 1396 1183 1010 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5476 5663 1700
Size: 110 3666 187 2869
Fragments arranged by size:
3666 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2505 3107 96
Size: 2409 602 3821
Fragments arranged by size:
3821 2409 602
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6832
BssSI C'ACGA_G
Cuts at: 3168 3227 4892 5904 3168
Size: 59 1665 1012 4096
Fragments arranged by size:
4096 1665 1012 59
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6832
BstBI TT'CG_AA
Cuts at: 5636 5636
Size: 6832
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6832
BstYI r'GATC_y
Cuts at: 1972 2368 3102 3290 3299 4162 4529 4914 4931
Size: 396 734 188 9 863 367 385 17
Cuts at: 4931 6383 6469 6481 6827 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 863 734 396 385 367 346
188 86 17 12 9
Bsu36I CC'TnA_GG
Cuts at: 3295 3295
Size: 6832
BtgI C'CryG_G
Cuts at: 513 1065 2944 2970 513
Size: 552 1879 26 4375
Fragments arranged by size:
4375 1879 552 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6093
Fragments arranged by size:
6093 683 56
BtsI GCAGTG_nn'
Cuts at: 2313 4348 5142 5162 2313
Size: 2035 794 20 3983
Fragments arranged by size:
3983 2035 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6832
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6797
DraI TTT'AAA
Cuts at: 2199 2359 3660 4136 2199
Size: 160 1301 476 4895
Fragments arranged by size:
4895 1301 476 160
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5839 809
Size: 507 4523 1802
Fragments arranged by size:
4523 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2967 3239 3260
Size: 54 1425 50 275 18 1137 272 21
Cuts at: 3260 3403 3421 4279 5168 6797 8
Size: 143 18 858 889 1629 43
Fragments arranged by size:
1629 1425 1137 889 858 275 272 143
54 50 43 21 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 4279 1537
Size: 275 2467 4090
Fragments arranged by size:
4090 2467 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2571 3090 4080 4109 4762 1353
Size: 1218 519 990 29 653 3423
Fragments arranged by size:
3423 1218 990 653 519 29
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5419 5793 5939 387
Size: 1064 138 3830 374 146 1280
Fragments arranged by size:
3830 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2253 2253
Size: 6832
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2063 2552 3480 3504 4895 5492 6279
Size: 393 431 489 928 24 1391 597 787
Cuts at: 6279 6547 1239
Size: 268 1524
Fragments arranged by size:
1524 1391 928 787 597 489 431 393
268 24
EcoRI G'AATT_C
Cuts at: 4141 4519 4141
Size: 378 6454
FspI TGC'GCA
Cuts at: 5338 5338
Size: 6832
HaeII r_GCGC'y
Cuts at: 1678 3751 5979 1678
Size: 2073 2228 2531
Fragments arranged by size:
2531 2228 2073
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3527 3559 5473 5505 5547
Size: 32 135 32 1955 32 1914 32 42
Cuts at: 5547 5579 1373
Size: 32 2626
Fragments arranged by size:
2626 1955 1914 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4176 4422 669
Size: 988 281 2238 246 3079
Fragments arranged by size:
3079 2238 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6832
HpaI GTT'AAC
Cuts at: 4422 4422
Size: 6832
KpnI G_GTAC'C
Cuts at: 4157 4157
Size: 6832
MfeI C'AATT_G
Cuts at: 4431 4431
Size: 6832
MluI A'CGCG_T
Cuts at: 6708 6708
Size: 6832
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2130 3841 5946 6130 1305
Size: 825 1711 2105 184 2007
Fragments arranged by size:
2105 2007 1711 825 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2969 3405 3423 10
Size: 54 1425 343 1137 436 18 3419
Fragments arranged by size:
3419 1425 1137 436 343 54 18
MslI CAynn'nnrTG
Cuts at: 518 1166 3558 4790 5149 5308 518
Size: 648 2392 1232 359 159 2042
Fragments arranged by size:
2392 2042 1232 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4248 4925 6073 6318 1606
Size: 2642 677 1148 245 2120
Fragments arranged by size:
2642 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2970 513
Size: 552 1905 4375
Fragments arranged by size:
4375 1905 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6832
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6832
NotI GC'GGCC_GC
Cuts at: 4279 4279
Size: 6832
NsiI A_TGCA'T
Cuts at: 2139 2544 2139
Size: 405 6427
NspI r_CATG'y
Cuts at: 1132 1229 2617 3890 4190 5735 1132
Size: 97 1388 1273 300 1545 2229
Fragments arranged by size:
2229 1545 1388 1273 300 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6832
PciI A'CATG_T
Cuts at: 5731 5731
Size: 6832
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6832
PmeI GTTT'AAAC
Cuts at: 4136 4136
Size: 6832
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4901 4933 6414 6446 4901
Size: 32 1481 32 5287
Fragments arranged by size:
5287 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6832
PsiI TTA'TAA
Cuts at: 4402 4402
Size: 6832
PstI C_TGCA'G
Cuts at: 830 4184 830
Size: 3354 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6832
SacI G_AGCT'C
Cuts at: 2255 2255
Size: 6832
SalI G'TCGA_C
Cuts at: 1936 4174 1936
Size: 2238 4594
SbfI CC_TGCA'GG
Cuts at: 4184 4184
Size: 6832
ScaI AGT'ACT
Cuts at: 5080 6792 5080
Size: 1712 5120
SfcI C'TryA_G
Cuts at: 826 1045 4180 5315 5996 6187 826
Size: 219 3135 1135 681 191 1471
Fragments arranged by size:
3135 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6832
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6832
SmaI CCC'GGG
Cuts at: 1872 3599 4159 1872
Size: 1727 560 4545
Fragments arranged by size:
4545 1727 560
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3139 4936 5837 6099 820
Size: 197 926 1196 1797 901 262 1553
Fragments arranged by size:
1797 1553 1196 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4623 493
Size: 4130 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6832
SphI G_CATG'C
Cuts at: 1132 3890 4190 1132
Size: 2758 300 3774
Fragments arranged by size:
3774 2758 300
SrfI GCCC'GGGC
Cuts at: 3599 3599
Size: 6832
SspI AAT'ATT
Cuts at: 5 52 2689 4756 5
Size: 47 2637 2067 2081
Fragments arranged by size:
2637 2081 2067 47
StuI AGG'CCT
Cuts at: 1517 2964 1517
Size: 1447 5385
StyI C'CwwG_G
Cuts at: 513 1065 2970 3523 4271 513
Size: 552 1905 553 748 3074
Fragments arranged by size:
3074 1905 748 553 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4859 4876 5029 5214 5659 325
Size: 1255 3279 17 153 185 445 1498
Fragments arranged by size:
3279 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2505 2571 3107
Size: 274 80 33 51 1443 528 66 536
Cuts at: 3107 3467 5078 6790 96
Size: 360 1611 1712 138
Fragments arranged by size:
1712 1611 1443 536 528 360 274 138
80 66 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2800 2832 6792 6824 2800
Size: 32 3960 32 2808
Fragments arranged by size:
3960 2808 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2062 1316
Size: 746 6086
XbaI T'CTAG_A
Cuts at: 3331 4168 3331
Size: 837 5995
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6832
XmaI C'CCGG_G
Cuts at: 1870 3597 4157 1870
Size: 1727 560 4545
Fragments arranged by size:
4545 1727 560
XmnI GAAnn'nnTTC
Cuts at: 2400 4961 2400
Size: 2561 4271
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4640 276
Size: 53 83 186 936 3106 2468
Fragments arranged by size:
3106 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BsiWI BstXI BstZ17I DraIII EcoNI EcoO109I EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacII SanDI SapI SexAI SfiI SfoI SwaI XcmI