Restriction map for
pFN21AA1411
(Circular) (Six-base) MAPSORT of: pFN21AA1411.seq Check: 5758 from: 1 to: 9250
pFN21AA1411 9250 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7060 278
Size: 53 83 186 936 5524 2468
Fragments arranged by size:
5524 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3410 6593 1937
Size: 1473 3183 4594
Fragments arranged by size:
4594 3183 1473
Acc65I G'GTAC_C
Cuts at: 6571 6571
Size: 9250
AclI AA'CG_TT
Cuts at: 1563 7377 7750 1563
Size: 5814 373 3063
Fragments arranged by size:
5814 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2031 3279 3281 3357 5219 5609 5663 5757
Size: 399 1248 2 76 1862 390 54 94
Cuts at: 5757 7313 8697 8965 1632
Size: 1556 1384 268 1917
Fragments arranged by size:
1917 1862 1556 1384 1248 399 390 268
94 76 54 2
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9053
AflIII A'CryG_T
Cuts at: 1203 5165 6173 8149 9126 1203
Size: 3962 1008 1976 977 1327
Fragments arranged by size:
3962 1976 1327 1008 977
AgeI A'CCGG_T
Cuts at: 8081 8081
Size: 9250
AhdI GACnn_n'nnGTC
Cuts at: 5907 6340 7979 5907
Size: 433 1639 7178
Fragments arranged by size:
7178 1639 433
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9250
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9250
AlwNI CAG_nnn'CTG
Cuts at: 2968 3257 6993 8565 2968
Size: 289 3736 1572 3653
Fragments arranged by size:
3736 3653 1572 289
ApaLI G'TGCA_C
Cuts at: 3130 5765 7307 8463 3130
Size: 2635 1542 1156 3917
Fragments arranged by size:
3917 2635 1542 1156
ApoI r'AATT_y
Cuts at: 1493 2030 2109 2121 2223 2326 3285 3835 4451
Size: 537 79 12 102 103 959 550 616
Cuts at: 4451 4564 4744 4906 5522 6515 6559 6785 6937
Size: 113 180 162 616 993 44 226 152
Cuts at: 6937 9046 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 993 959 616 616 550 537
226 180 162 152 113 103 102 79
44 12
AseI AT'TA_AT
Cuts at: 160 1032 3530 7804 160
Size: 872 2498 4274 1606
Fragments arranged by size:
4274 2498 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9250
AvaI C'yCGr_G
Cuts at: 1870 1943 6575 1870
Size: 73 4632 4545
Fragments arranged by size:
4632 4545 73
BamHI G'GATC_C
Cuts at: 3655 6266 6580 6947 3655
Size: 2611 314 367 5958
Fragments arranged by size:
5958 2611 367 314
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3392 6571 8026 618
Size: 325 217 627 1605 3179 1455 1842
Fragments arranged by size:
3179 1842 1605 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 6569 1391
Size: 5178 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2570 3267 928
Size: 971 671 697 6911
Fragments arranged by size:
6911 971 697 671
BbvCI CC'TCA_GC
Cuts at: 4419 4419
Size: 9250
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4621 4655 7441 7475 1903
Size: 34 2684 34 2786 34 3678
Fragments arranged by size:
3678 2786 2684 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4831 7142 8352 4831
Size: 2311 1210 5729
Fragments arranged by size:
5729 2311 1210
BclI T'GATC_A
Cuts at: 1548 3937 1548
Size: 2389 6861
BfrBI ATG'CAT
Cuts at: 2513 6437 2513
Size: 3924 5326
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7861 136
Size: 107 122 71 631 605 6189 1525
Fragments arranged by size:
6189 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3205 5791 6351 9245 3205
Size: 2586 560 2894 3210
Fragments arranged by size:
3210 2894 2586 560
BlpI GC'TnA_GC
Cuts at: 6667 6667
Size: 9250
Bme1580I G_kGCm'C
Cuts at: 1790 3134 5769 7311 8467 1790
Size: 1344 2635 1542 1156 2573
Fragments arranged by size:
2635 2573 1542 1344 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9250
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3981 6981 7939
Size: 416 53 83 399 24 2564 3000 958
Cuts at: 7939 442
Size: 1753
Fragments arranged by size:
3000 2564 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2756 3726 1056
Size: 1700 970 6580
Fragments arranged by size:
6580 1700 970
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2846 3382 3750 7910 1239
Size: 1607 536 368 4160 2579
Fragments arranged by size:
4160 2579 1607 536 368
Bpu10I CC'TnA_GC
Cuts at: 4419 4419
Size: 9250
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2336 7375 8240 8538 2336
Size: 5039 865 298 3048
Fragments arranged by size:
5039 3048 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6128 7913 882
Size: 617 4629 1785 2219
Fragments arranged by size:
4629 2219 1785 617
BsaAI yAC'GTr
Cuts at: 493 1204 7041 493
Size: 711 5837 2702
Fragments arranged by size:
5837 2702 711
BsaBI GATnn'nnATC
Cuts at: 3204 9244 3204
Size: 6040 3210
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 7057 7439 8074 275
Size: 53 83 186 936 5524 382 635 1451
Fragments arranged by size:
5524 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 7683 8081 8355 8502 8913 7683
Size: 398 274 147 411 8020
Fragments arranged by size:
8020 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 9025
Fragments arranged by size:
9025 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 5276 1186
Size: 4090 5160
BseYI C'CCAG_C
Cuts at: 8453 8949 8453
Size: 496 8754
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2408 5460 5808 6459 1163
Size: 573 672 3052 348 651 3954
Fragments arranged by size:
3954 3052 672 651 573 348
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6700 7461 8489 1540
Size: 275 186 4699 761 1028 2301
Fragments arranged by size:
4699 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3021 3134 5769 6109 7311 7396 8467 3021
Size: 113 2635 340 1202 85 1071 3804
Fragments arranged by size:
3804 2635 1202 1071 340 113 85
BsmI GAATG_Cn'
Cuts at: 2392 3053 4595 6761 6854 2392
Size: 661 1542 2166 93 4788
Fragments arranged by size:
4788 2166 1542 661 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3021 3134 5769 6109 6569 7311 7396
Size: 399 1231 113 2635 340 460 742 85
Cuts at: 7396 8467 1391
Size: 1071 2174
Fragments arranged by size:
2635 2174 1231 1071 742 460 399 340
113 85
BspEI T'CCGG_A
Cuts at: 8913 8913
Size: 9250
BspHI T'CATG_A
Cuts at: 2191 7139 8869 2191
Size: 4948 1730 2572
Fragments arranged by size:
4948 2572 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3394 4222 6605 844
Size: 2550 828 2383 3489
Fragments arranged by size:
3489 2550 2383 828
BsrBI CCG'CTC
Cuts at: 1394 1823 7137 1394
Size: 429 5314 3507
Fragments arranged by size:
5314 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2028 2688 3087 5191 6437 7745 7919
Size: 1183 780 660 399 2104 1246 1308 174
Cuts at: 7919 65
Size: 1396
Fragments arranged by size:
2104 1396 1308 1246 1183 780 660 399
174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7894 8081 1700
Size: 110 6084 187 2869
Fragments arranged by size:
6084 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2229 5670 96
Size: 2133 3441 3676
Fragments arranged by size:
3676 3441 2133
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9250
BssSI C'ACGA_G
Cuts at: 5481 7310 8322 5481
Size: 1829 1012 6409
Fragments arranged by size:
6409 1829 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 9250
BstBI TT'CG_AA
Cuts at: 8054 8054
Size: 9250
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9250
BstXI CCAn_nnnn'nTGG
Cuts at: 3319 3319
Size: 9250
BstYI r'GATC_y
Cuts at: 1972 2344 3205 3655 3997 5791 6266 6351 6580
Size: 372 861 450 342 1794 475 85 229
Cuts at: 6580 6947 7332 7349 8801 8887 8899 9245 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1794 1452 861 475 450 385 372
367 346 342 229 86 85 17 12
BstZ17I GTA'TAC
Cuts at: 3411 3411
Size: 9250
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 8698
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8511
Fragments arranged by size:
8511 683 56
BtsI GCAGTG_nn'
Cuts at: 6109 6766 7560 7580 6109
Size: 657 794 20 7779
Fragments arranged by size:
7779 794 657 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9250
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9215
DraI TTT'AAA
Cuts at: 2253 6031 6330 6554 2253
Size: 3778 299 224 4949
Fragments arranged by size:
4949 3778 299 224
DraIII CAC_nnn'GTG
Cuts at: 2738 2738
Size: 9250
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3708 8257 809
Size: 507 2392 4549 1802
Fragments arranged by size:
4549 2392 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 6697 7586 9215
Size: 54 1425 50 275 18 4867 889 1629
Cuts at: 9215 8
Size: 43
Fragments arranged by size:
4867 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 6697 1537
Size: 275 4885 4090
Fragments arranged by size:
4885 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2235 2790 3171 4782 5262 5718 7180 1353
Size: 882 555 381 1611 480 456 1462 3423
Fragments arranged by size:
3423 1611 1462 882 555 480 456 381
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7837 8211 8357 387
Size: 1064 138 6248 374 146 1280
Fragments arranged by size:
6248 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2031 2846 3279 3281 3357 3382 3750
Size: 393 399 815 433 2 76 25 368
Cuts at: 3750 5219 5609 5663 5757 7313 7910 8697 8965
Size: 1469 390 54 94 1556 597 787 268
Cuts at: 8965 1239
Size: 1524
Fragments arranged by size:
1556 1524 1469 815 787 597 433 399
393 390 368 268 94 76 54 25
2
EcoO109I rG'GnC_Cy
Cuts at: 4034 4391 4034
Size: 357 8893
EcoRI G'AATT_C
Cuts at: 2030 2121 3285 6559 6937 2030
Size: 91 1164 3274 378 4343
Fragments arranged by size:
4343 3274 1164 378 91
EcoRV GAT'ATC
Cuts at: 3862 3862
Size: 9250
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2330 2443 2867 4692 5097 5399 5571 5639 6251
Size: 113 424 1825 405 302 172 68 612
Cuts at: 6251 2330
Size: 5329
Fragments arranged by size:
5329 1825 612 424 405 302 172 113
68
FspI TGC'GCA
Cuts at: 2928 7756 2928
Size: 4828 4422
HaeII r_GCGC'y
Cuts at: 1678 8397 1678
Size: 6719 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2605 2637 4535 4567 5893
Size: 32 135 32 1033 32 1898 32 1326
Cuts at: 5893 5925 5932 5964 6326 6358 7891 7923 7965
Size: 32 7 32 362 32 1533 32 42
Cuts at: 7965 7997 1373
Size: 32 2626
Fragments arranged by size:
2626 1898 1533 1326 1033 362 135 42
32 32 32 32 32 32 32 32
32 7
HincII GTy'rAC
Cuts at: 669 1657 1938 6594 6840 669
Size: 988 281 4656 246 3079
Fragments arranged by size:
4656 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2545 3491 4149 5968 6215 748
Size: 1797 946 658 1819 247 3783
Fragments arranged by size:
3783 1819 1797 946 658 247
HpaI GTT'AAC
Cuts at: 6840 6840
Size: 9250
KpnI G_GTAC'C
Cuts at: 6575 6575
Size: 9250
MfeI C'AATT_G
Cuts at: 3972 6849 3972
Size: 2877 6373
MluI A'CGCG_T
Cuts at: 9126 9126
Size: 9250
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 8364 8548 1305
Size: 7059 184 2007
Fragments arranged by size:
7059 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 7428
Fragments arranged by size:
7428 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2454 2460 4599 5024 6311 7208 7567
Size: 648 1288 6 2139 425 1287 897 359
Cuts at: 7567 7726 518
Size: 159 2042
Fragments arranged by size:
2139 2042 1288 1287 897 648 425 359
159 6
MspA1I CmG'CkG
Cuts at: 1606 6169 6452 6666 7343 8491 8736 1606
Size: 4563 283 214 677 1148 245 2120
Fragments arranged by size:
4563 2120 1148 677 283 245 214
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 8698
NdeI CA'TA_TG
Cuts at: 387 4288 387
Size: 3901 5349
NheI G'CTAG_C
Cuts at: 1052 2752 3722 1052
Size: 1700 970 6580
Fragments arranged by size:
6580 1700 970
NotI GC'GGCC_GC
Cuts at: 6697 6697
Size: 9250
NsiI A_TGCA'T
Cuts at: 2515 6439 2515
Size: 3924 5326
NspI r_CATG'y
Cuts at: 1132 1229 2156 5169 5437 6177 6608 8153 1132
Size: 97 927 3013 268 740 431 1545 2229
Fragments arranged by size:
3013 2229 1545 927 740 431 268 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9250
PciI A'CATG_T
Cuts at: 5165 6173 8149 5165
Size: 1008 1976 6266
Fragments arranged by size:
6266 1976 1008
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3405 1108
Size: 2297 6953
PmeI GTTT'AAAC
Cuts at: 6554 6554
Size: 9250
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7319 7351 8832 8864 7319
Size: 32 1481 32 7705
Fragments arranged by size:
7705 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4034 4391 4034
Size: 357 8893
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9250
PsiI TTA'TAA
Cuts at: 2912 2978 4443 6820 2912
Size: 66 1465 2377 5342
Fragments arranged by size:
5342 2377 1465 66
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5631 5663 5631
Size: 32 9218
PstI C_TGCA'G
Cuts at: 830 3161 6602 830
Size: 2331 3441 3478
Fragments arranged by size:
3478 3441 2331
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9250
PvuII CAG'CTG
Cuts at: 6169 6452 6169
Size: 283 8967
SalI G'TCGA_C
Cuts at: 1936 6592 1936
Size: 4656 4594
SapI GCTCTTCn'nnn_
Cuts at: 5262 5262
Size: 9250
SbfI CC_TGCA'GG
Cuts at: 6602 6602
Size: 9250
ScaI AGT'ACT
Cuts at: 5095 5554 7498 9210 5095
Size: 459 1944 1712 5135
Fragments arranged by size:
5135 1944 1712 459
SfcI C'TryA_G
Cuts at: 826 1045 3122 3157 3348 4125 4725 4884 5043
Size: 219 2077 35 191 777 600 159 159
Cuts at: 5043 5179 6272 6598 7733 8414 8605 826
Size: 136 1093 326 1135 681 191 1471
Fragments arranged by size:
2077 1471 1135 1093 777 681 600 326
219 191 191 159 159 136 35
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9250
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9250
SmaI CCC'GGG
Cuts at: 1872 6577 1872
Size: 4705 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2315 7354 8255 8517 820
Size: 197 926 372 5039 901 262 1553
Fragments arranged by size:
5039 1553 926 901 372 262 197
SnaBI TAC'GTA
Cuts at: 493 7041 493
Size: 6548 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9250
SphI G_CATG'C
Cuts at: 1132 2156 6608 1132
Size: 1024 4452 3774
Fragments arranged by size:
4452 3774 1024
SspI AAT'ATT
Cuts at: 5 52 3003 5507 6035 6504 6542 7174 5
Size: 47 2951 2504 528 469 38 632 2081
Fragments arranged by size:
2951 2504 2081 632 528 469 47 38
StuI AGG'CCT
Cuts at: 1517 2445 1517
Size: 928 8322
StyI C'CwwG_G
Cuts at: 513 1065 2340 4037 6023 6082 6689 513
Size: 552 1275 1697 1986 59 607 3074
Fragments arranged by size:
3074 1986 1697 1275 607 552 59
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 7277 7294 7447 7632 8077 325
Size: 1255 5697 17 153 185 445 1498
Fragments arranged by size:
5697 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2229 3587 4472
Size: 274 80 33 51 1443 252 1358 885
Cuts at: 4472 4481 5093 5447 5552 5670 5746 7496 9208
Size: 9 612 354 105 118 76 1750 1712
Cuts at: 9208 96
Size: 138
Fragments arranged by size:
1750 1712 1443 1358 885 612 354 274
252 138 118 105 80 76 51 33
9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4829 4861 9210 9242 4829
Size: 32 4349 32 4837
Fragments arranged by size:
4837 4349 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9250
XbaI T'CTAG_A
Cuts at: 4298 6586 4298
Size: 2288 6962
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4831 4831
Size: 9250
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9250
XmaI C'CCGG_G
Cuts at: 1870 6575 1870
Size: 4705 4545
XmnI GAAnn'nnTTC
Cuts at: 2799 3780 7379 2799
Size: 981 3599 4670
Fragments arranged by size:
4670 3599 981
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7058 276
Size: 53 83 186 936 5524 2468
Fragments arranged by size:
5524 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BsmBI Bsu36I EcoICRI EcoNI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI
PspOMI PspXI RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI