Restriction map for
pFN21AA1408
(Circular) (Six-base) MAPSORT of: pFN21AA1408.seq Check: 3293 from: 1 to: 8521
pFN21AA1408 8521 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6331 278
Size: 53 83 186 936 4795 2468
Fragments arranged by size:
4795 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2941 5864 1937
Size: 1004 2923 4594
Fragments arranged by size:
4594 2923 1004
Acc65I G'GTAC_C
Cuts at: 5842 5842
Size: 8521
AclI AA'CG_TT
Cuts at: 1563 6648 7021 1563
Size: 5085 373 3063
Fragments arranged by size:
5085 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2184 2470 2711 3645 6584 7968 8236 1632
Size: 552 286 241 934 2939 1384 268 1917
Fragments arranged by size:
2939 1917 1384 934 552 286 268 241
AflII C'TTAA_G
Cuts at: 820 1017 5309 820
Size: 197 4292 4032
Fragments arranged by size:
4292 4032 197
AflIII A'CryG_T
Cuts at: 1203 4130 4787 5818 7420 8397 1203
Size: 2927 657 1031 1602 977 1327
Fragments arranged by size:
2927 1602 1327 1031 977 657
AgeI A'CCGG_T
Cuts at: 7352 7352
Size: 8521
AhdI GACnn_n'nnGTC
Cuts at: 2569 7250 2569
Size: 4681 3840
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8521
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4242 5724 1184
Size: 3058 1482 3981
Fragments arranged by size:
3981 3058 1482
AlwNI CAG_nnn'CTG
Cuts at: 2618 3229 6264 7836 2618
Size: 611 3035 1572 3303
Fragments arranged by size:
3303 3035 1572 611
ApaI G_GGCC'C
Cuts at: 5466 5466
Size: 8521
ApaLI G'TGCA_C
Cuts at: 2672 5408 6578 7734 2672
Size: 2736 1170 1156 3459
Fragments arranged by size:
3459 2736 1170 1156
ApoI r'AATT_y
Cuts at: 1493 2593 3206 3238 3400 4592 5021 5830 6056
Size: 1100 613 32 162 1192 429 809 226
Cuts at: 6056 6208 8317 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1192 1100 809 613 429 226
162 152 32
AseI AT'TA_AT
Cuts at: 160 1032 2889 3585 7075 160
Size: 872 1857 696 3490 1606
Fragments arranged by size:
3490 1857 1606 872 696
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8521
AvaI C'yCGr_G
Cuts at: 1870 1943 5846 1870
Size: 73 3903 4545
Fragments arranged by size:
4545 3903 73
BamHI G'GATC_C
Cuts at: 4530 5851 6218 4530
Size: 1321 367 6833
Fragments arranged by size:
6833 1321 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5842 7297 618
Size: 325 217 627 4055 1455 1842
Fragments arranged by size:
4055 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3126 3177 5466 5840 1391
Size: 1735 51 2289 374 4072
Fragments arranged by size:
4072 2289 1735 374 51
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2172 2214 928
Size: 971 273 42 7235
Fragments arranged by size:
7235 971 273 42
BbvCI CC'TCA_GC
Cuts at: 3935 5453 3935
Size: 1518 7003
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 6712 6746 1903
Size: 34 50 34 4691 34 3678
Fragments arranged by size:
4691 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6413 7623 6413
Size: 1210 7311
BclI T'GATC_A
Cuts at: 1548 4929 1548
Size: 3381 5140
BfrBI ATG'CAT
Cuts at: 4219 4256 4219
Size: 37 8484
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7132 136
Size: 107 122 71 631 605 5460 1525
Fragments arranged by size:
5460 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3280 3628 3748 4151 5788 8516 3280
Size: 348 120 403 1637 2728 3285
Fragments arranged by size:
3285 2728 1637 403 348 120
BlpI GC'TnA_GC
Cuts at: 2013 5938 2013
Size: 3925 4596
Bme1580I G_kGCm'C
Cuts at: 1790 2676 3826 5412 5466 6582 7738 1790
Size: 886 1150 1586 54 1116 1156 2573
Fragments arranged by size:
2573 1586 1156 1150 1116 886 54
BmgBI CAC'GTC
Cuts at: 1318 5819 1318
Size: 4501 4020
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5480 6252 7210
Size: 416 53 83 399 24 4063 772 958
Cuts at: 7210 442
Size: 1753
Fragments arranged by size:
4063 1753 958 772 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8521
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3036 5769 3036
Size: 2733 5788
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3110 3372 4140 4145 4263 4301 5709 7181
Size: 1871 262 768 5 118 38 1408 1472
Cuts at: 7181 1239
Size: 2579
Fragments arranged by size:
2579 1871 1472 1408 768 262 118 38
5
Bpu10I CC'TnA_GC
Cuts at: 3250 3935 4743 5453 3250
Size: 685 808 710 6318
Fragments arranged by size:
6318 808 710 685
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2485 3273 3584 3680 6646 7511 7809 2485
Size: 788 311 96 2966 865 298 3197
Fragments arranged by size:
3197 2966 865 788 311 298 96
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 7184 882
Size: 617 5685 2219
Fragments arranged by size:
5685 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 6312 493
Size: 711 5108 2702
Fragments arranged by size:
5108 2702 711
BsaBI GATnn'nnATC
Cuts at: 4448 8515 4448
Size: 4067 4454
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6328 6710 7345 275
Size: 53 83 186 936 4795 382 635 1451
Fragments arranged by size:
4795 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2458 4329 6954 7352 7626 7773 8184 2458
Size: 1871 2625 398 274 147 411 2795
Fragments arranged by size:
2795 2625 1871 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 5803 5833 1178
Size: 30 165 30 4400 30 3866
Fragments arranged by size:
4400 3866 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2777 4302 5192 5231 5827 1186
Size: 1591 1525 890 39 596 3880
Fragments arranged by size:
3880 1591 1525 890 596 39
BseYI C'CCAG_C
Cuts at: 3439 5719 7724 8220 3439
Size: 2280 2005 496 3740
Fragments arranged by size:
3740 2280 2005 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7948
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5971 6732 7760 1540
Size: 275 186 3970 761 1028 2301
Fragments arranged by size:
3970 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2676 3126 3177 4512 5412 5779 6582 6667 7738
Size: 450 51 1335 900 367 803 85 1071
Cuts at: 7738 2676
Size: 3459
Fragments arranged by size:
3459 1335 1071 900 803 450 367 85
51
BsmI GAATG_Cn'
Cuts at: 3153 4258 6032 6125 3153
Size: 1105 1774 93 5549
Fragments arranged by size:
5549 1774 1105 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5809 5809
Size: 8521
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2676 3126 3177 3826 4512 5412 5466
Size: 399 886 450 51 649 686 900 54
Cuts at: 5466 5779 5840 6582 6667 7738 1391
Size: 313 61 742 85 1071 2174
Fragments arranged by size:
2174 1071 900 886 742 686 649 450
399 313 85 61 54 51
BspEI T'CCGG_A
Cuts at: 2458 4329 8184 2458
Size: 1871 3855 2795
Fragments arranged by size:
3855 2795 1871
BspHI T'CATG_A
Cuts at: 6410 8140 6410
Size: 1730 6791
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4990 5876 844
Size: 4146 886 3489
Fragments arranged by size:
4146 3489 886
BsrBI CCG'CTC
Cuts at: 1394 1823 6408 1394
Size: 429 4585 3507
Fragments arranged by size:
4585 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2607 3774 3887 4221 7016 7190 65
Size: 1183 1359 1167 113 334 2795 174 1396
Fragments arranged by size:
2795 1396 1359 1183 1167 334 174 113
BsrFI r'CCGG_y
Cuts at: 1700 1810 7165 7352 1700
Size: 110 5355 187 2869
Fragments arranged by size:
5355 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4341 96
Size: 4245 4276
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8521
BssSI C'ACGA_G
Cuts at: 6581 7593 6581
Size: 1012 7509
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4216 5783 794
Size: 3422 1567 3532
Fragments arranged by size:
3532 3422 1567
BstBI TT'CG_AA
Cuts at: 7325 7325
Size: 8521
BstEII G'GTnAC_C
Cuts at: 1184 4360 1184
Size: 3176 5345
BstYI r'GATC_y
Cuts at: 1972 3280 3628 3686 3748 3919 4151 4348 4530
Size: 1308 348 58 62 171 232 197 182
Cuts at: 4530 5556 5788 5851 6218 6603 6620 8072 8158
Size: 1026 232 63 367 385 17 1452 86
Cuts at: 8158 8170 8516 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1308 1026 385 367 348 346
232 232 197 182 171 86 63 62
58 17 12
Bsu36I CC'TnA_GG
Cuts at: 4169 4169
Size: 8521
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 7969
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7782
Fragments arranged by size:
7782 683 56
BtsI GCAGTG_nn'
Cuts at: 2086 2133 4055 4621 6037 6831 6851 2086
Size: 47 1922 566 1416 794 20 3756
Fragments arranged by size:
3756 1922 1416 794 566 47 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8521
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8486
DraI TTT'AAA
Cuts at: 3072 3454 4068 5825 3072
Size: 382 614 1757 5768
Fragments arranged by size:
5768 1757 614 382
DraIII CAC_nnn'GTG
Cuts at: 4540 4540
Size: 8521
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7528 809
Size: 507 6212 1802
Fragments arranged by size:
6212 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4792 5968 6857
Size: 54 1425 50 275 18 2962 1176 889
Cuts at: 6857 8486 8
Size: 1629 43
Fragments arranged by size:
2962 1629 1425 1176 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5968 1537
Size: 275 4156 4090
Fragments arranged by size:
4156 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2171 2268 2298 2316 3833 5088 5169 5208
Size: 818 97 30 18 1517 1255 81 39
Cuts at: 5208 5217 5268 5619 6451 1353
Size: 9 51 351 832 3423
Fragments arranged by size:
3423 1517 1255 832 818 351 97 81
51 39 30 18 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2104 7108 7482 7628 387
Size: 1064 138 515 5004 374 146 1280
Fragments arranged by size:
5004 1280 1064 515 374 146 138
EcoICRI GAG'CTC
Cuts at: 3124 3175 3124
Size: 51 8470
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2184 2470 2711 3110 3372 3645 4140
Size: 393 552 286 241 399 262 273 495
Cuts at: 4140 4145 4263 4301 5709 6584 7181 7968 8236
Size: 5 118 38 1408 875 597 787 268
Cuts at: 8236 1239
Size: 1524
Fragments arranged by size:
1524 1408 875 787 597 552 495 399
393 286 273 268 262 241 118 38
5
EcoO109I rG'GnC_Cy
Cuts at: 2158 5462 5463 2158
Size: 3304 1 5216
Fragments arranged by size:
5216 3304 1
EcoRI G'AATT_C
Cuts at: 5021 5830 6208 5021
Size: 809 378 7334
Fragments arranged by size:
7334 809 378
EcoRV GAT'ATC
Cuts at: 2125 5659 2125
Size: 3534 4987
FspI TGC'GCA
Cuts at: 7027 7027
Size: 8521
HaeII r_GCGC'y
Cuts at: 1678 5719 7668 1678
Size: 4041 1949 2531
Fragments arranged by size:
4041 2531 1949
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2243 2275 2555 2587 2888
Size: 32 135 32 671 32 280 32 301
Cuts at: 2888 2920 3022 3054 3678 3710 4494 4526 4947
Size: 32 102 32 624 32 784 32 421
Cuts at: 4947 4979 5042 5074 5480 5512 7162 7194 7236
Size: 32 63 32 406 32 1650 32 42
Cuts at: 7236 7268 1373
Size: 32 2626
Fragments arranged by size:
2626 1650 784 671 624 421 406 301
280 135 102 63 42 32 32 32
32 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2942 3742 5526 5865 6111 669
Size: 988 281 1004 800 1784 339 246 3079
Fragments arranged by size:
3079 1784 1004 988 800 339 281 246
HindIII A'AGCT_T
Cuts at: 748 2403 748
Size: 1655 6866
HpaI GTT'AAC
Cuts at: 5526 6111 5526
Size: 585 7936
KpnI G_GTAC'C
Cuts at: 5846 5846
Size: 8521
MfeI C'AATT_G
Cuts at: 6120 6120
Size: 8521
MluI A'CGCG_T
Cuts at: 8397 8397
Size: 8521
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3513 3549 3831 7635 7819 1305
Size: 2208 36 282 3804 184 2007
Fragments arranged by size:
3804 2208 2007 282 184 36
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4794 10
Size: 54 1425 343 2962 3737
Fragments arranged by size:
3737 2962 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2913 3785 4224 6479 6838 6997 518
Size: 648 1747 872 439 2255 359 159 2042
Fragments arranged by size:
2255 2042 1747 872 648 439 359 159
MspA1I CmG'CkG
Cuts at: 1606 2012 2357 4283 4664 5452 5937 6614 7762
Size: 406 345 1926 381 788 485 677 1148
Cuts at: 7762 8007 1606
Size: 245 2120
Fragments arranged by size:
2120 1926 1148 788 677 485 406 381
345 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7969
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8521
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8521
NotI GC'GGCC_GC
Cuts at: 5968 5968
Size: 8521
NsiI A_TGCA'T
Cuts at: 4221 4258 4221
Size: 37 8484
NspI r_CATG'y
Cuts at: 1132 1229 3904 4116 4134 4791 5318 5879 7424
Size: 97 2675 212 18 657 527 561 1545
Cuts at: 7424 1132
Size: 2229
Fragments arranged by size:
2675 2229 1545 657 561 527 212 97
18
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8521
PciI A'CATG_T
Cuts at: 4130 4787 7420 4130
Size: 657 2633 5231
Fragments arranged by size:
5231 2633 657
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4540 1108
Size: 3432 5089
PfoI T'CCnGG_A
Cuts at: 4317 4317
Size: 8521
PmeI GTTT'AAAC
Cuts at: 5825 5825
Size: 8521
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6590 6622 8103 8135 6590
Size: 32 1481 32 6976
Fragments arranged by size:
6976 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8521
PsiI TTA'TAA
Cuts at: 6091 6091
Size: 8521
PspOMI G'GGCC_C
Cuts at: 5462 5462
Size: 8521
PstI C_TGCA'G
Cuts at: 830 2081 2998 5503 5873 830
Size: 1251 917 2505 370 3478
Fragments arranged by size:
3478 2505 1251 917 370
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8521
PvuII CAG'CTG
Cuts at: 2012 2357 4283 4664 5452 2012
Size: 345 1926 381 788 5081
Fragments arranged by size:
5081 1926 788 381 345
SacI G_AGCT'C
Cuts at: 3126 3177 3126
Size: 51 8470
SalI G'TCGA_C
Cuts at: 1936 2940 5863 1936
Size: 1004 2923 4594
Fragments arranged by size:
4594 2923 1004
SapI GCTCTTCn'nnn_
Cuts at: 2316 2316
Size: 8521
SbfI CC_TGCA'GG
Cuts at: 5873 5873
Size: 8521
ScaI AGT'ACT
Cuts at: 3080 4799 6769 8481 3080
Size: 1719 1970 1712 3120
Fragments arranged by size:
3120 1970 1719 1712
SexAI A'CCwGG_T
Cuts at: 3340 3340
Size: 8521
SfcI C'TryA_G
Cuts at: 826 1045 2077 2619 2922 2994 3719 5378 5499
Size: 219 1032 542 303 72 725 1659 121
Cuts at: 5499 5869 7004 7685 7876 826
Size: 370 1135 681 191 1471
Fragments arranged by size:
1659 1471 1135 1032 725 681 542 370
303 219 191 121 72
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8521
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8521
SmaI CCC'GGG
Cuts at: 1872 5848 1872
Size: 3976 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2500 3288 3563 3695 5309 6625
Size: 197 926 557 788 275 132 1614 1316
Cuts at: 6625 7526 7788 820
Size: 901 262 1553
Fragments arranged by size:
1614 1553 1316 926 901 788 557 275
262 197 132
SnaBI TAC'GTA
Cuts at: 493 6312 493
Size: 5819 2702
SpeI A'CTAG_T
Cuts at: 152 4504 152
Size: 4352 4169
SphI G_CATG'C
Cuts at: 1132 4116 5879 1132
Size: 2984 1763 3774
Fragments arranged by size:
3774 2984 1763
SspI AAT'ATT
Cuts at: 5 52 2884 3982 6445 5
Size: 47 2832 1098 2463 2081
Fragments arranged by size:
2832 2463 2081 1098 47
StuI AGG'CCT
Cuts at: 1517 5040 1517
Size: 3523 4998
StyI C'CwwG_G
Cuts at: 513 1065 2975 5960 513
Size: 552 1910 2985 3074
Fragments arranged by size:
3074 2985 1910 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 6548 6565 6718 6903 7348 325
Size: 1255 4968 17 153 185 445 1498
Fragments arranged by size:
4968 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3078 4180 4341
Size: 274 80 33 51 1443 1101 1102 161
Cuts at: 4341 4797 6767 8479 96
Size: 456 1970 1712 138
Fragments arranged by size:
1970 1712 1443 1102 1101 456 274 161
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5803 5835 8481 8513 5803
Size: 32 2646 32 5811
Fragments arranged by size:
5811 2646 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8521
XbaI T'CTAG_A
Cuts at: 3577 5857 3577
Size: 2280 6241
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2582 4183 4523 4541 2582
Size: 1601 340 18 6562
Fragments arranged by size:
6562 1601 340 18
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8521
XmaI C'CCGG_G
Cuts at: 1870 5846 1870
Size: 3976 4545
XmnI GAAnn'nnTTC
Cuts at: 2385 4560 6650 2385
Size: 2175 2090 4256
Fragments arranged by size:
4256 2175 2090
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6329 276
Size: 53 83 186 936 4795 2468
Fragments arranged by size:
4795 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BsiWI
BstXI BstZ17I EcoNI FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PmlI PpuMI PspXI PsrI
RsrII SacII SanDI SfiI SfoI SrfI SwaI