Restriction map for
pFN21AA1401
(Circular) (Six-base) MAPSORT of: pFN21AA1401.seq Check: 2589 from: 1 to: 7117
pFN21AA1401 7117 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4927 278
Size: 53 83 186 936 3391 2468
Fragments arranged by size:
3391 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2157 3055 4460 1937
Size: 220 898 1405 4594
Fragments arranged by size:
4594 1405 898 220
Acc65I G'GTAC_C
Cuts at: 4241 4438 4241
Size: 197 6920
AclI AA'CG_TT
Cuts at: 1563 5244 5617 1563
Size: 3681 373 3063
Fragments arranged by size:
3681 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2615 3706 3714 5180 6564 6832 1632
Size: 983 1091 8 1466 1384 268 1917
Fragments arranged by size:
1917 1466 1384 1091 983 268 8
AfeI AGC'GCT
Cuts at: 2645 2645
Size: 7117
AflII C'TTAA_G
Cuts at: 820 1017 3521 820
Size: 197 2504 4416
Fragments arranged by size:
4416 2504 197
AflIII A'CryG_T
Cuts at: 1203 4149 6016 6993 1203
Size: 2946 1867 977 1327
Fragments arranged by size:
2946 1867 1327 977
AgeI A'CCGG_T
Cuts at: 2510 5948 2510
Size: 3438 3679
AhdI GACnn_n'nnGTC
Cuts at: 5846 5846
Size: 7117
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7117
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4043 1184
Size: 2859 4258
AlwNI CAG_nnn'CTG
Cuts at: 2260 2851 3050 4860 6432 2260
Size: 591 199 1810 1572 2945
Fragments arranged by size:
2945 1810 1572 591 199
ApaLI G'TGCA_C
Cuts at: 5174 6330 5174
Size: 1156 5961
ApoI r'AATT_y
Cuts at: 1493 2283 2823 3495 4119 4426 4652 4804 6913
Size: 790 540 672 624 307 226 152 2109
Cuts at: 6913 1493
Size: 1697
Fragments arranged by size:
2109 1697 790 672 624 540 307 226
152
AseI AT'TA_AT
Cuts at: 160 1032 5671 160
Size: 872 4639 1606
Fragments arranged by size:
4639 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7117
AvaI C'yCGr_G
Cuts at: 1870 1943 4442 1870
Size: 73 2499 4545
Fragments arranged by size:
4545 2499 73
BamHI G'GATC_C
Cuts at: 3547 4447 4814 3547
Size: 900 367 5850
Fragments arranged by size:
5850 900 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2266 2813 4241 4438 5893
Size: 325 217 627 479 547 1428 197 1455
Cuts at: 5893 618
Size: 1842
Fragments arranged by size:
1842 1455 1428 627 547 479 325 217
197
BanII G_rGCy'C
Cuts at: 1391 3846 4436 1391
Size: 2455 590 4072
Fragments arranged by size:
4072 2455 590
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4315 928
Size: 971 2416 3730
Fragments arranged by size:
3730 2416 971
BbvCI CC'TCA_GC
Cuts at: 2413 3405 3660 2413
Size: 992 255 5870
Fragments arranged by size:
5870 992 255
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2054 2088 5308 5342 1903
Size: 34 117 34 3220 34 3678
Fragments arranged by size:
3678 3220 117 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5009 6219 5009
Size: 1210 5907
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7117
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3956 5728
Size: 107 122 71 631 605 337 1947 1772
Cuts at: 5728 136
Size: 1525
Fragments arranged by size:
1947 1772 1525 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 7112 7112
Size: 7117
BlpI GC'TnA_GC
Cuts at: 3131 4534 3131
Size: 1403 5714
Bme1580I G_kGCm'C
Cuts at: 1790 2271 2384 2816 5178 6334 1790
Size: 481 113 432 2362 1156 2573
Fragments arranged by size:
2573 2362 1156 481 432 113
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7117
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4848 5806 442
Size: 416 53 83 399 24 3431 958 1753
Fragments arranged by size:
3431 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7117
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4234 5777 1239
Size: 2995 1543 2579
Fragments arranged by size:
2995 2579 1543
Bpu10I CC'TnA_GC
Cuts at: 2123 2413 2541 2702 3405 3660 2123
Size: 290 128 161 703 255 5580
Fragments arranged by size:
5580 703 290 255 161 128
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2015 3552 4272 4386 5242 6107 6405 2015
Size: 1537 720 114 856 865 298 2727
Fragments arranged by size:
2727 1537 865 856 720 298 114
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2916 5780 882
Size: 617 1417 2864 2219
Fragments arranged by size:
2864 2219 1417 617
BsaAI yAC'GTr
Cuts at: 493 1204 4908 493
Size: 711 3704 2702
Fragments arranged by size:
3704 2702 711
BsaBI GATnn'nnATC
Cuts at: 7111 7111
Size: 7117
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4924 5306 5941 275
Size: 53 83 186 936 3391 382 635 1451
Fragments arranged by size:
3391 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2510 3531 5550 5948 6222 6369 6780 2510
Size: 1021 2019 398 274 147 411 2847
Fragments arranged by size:
2847 2019 1021 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3648 3678 3970 4000 1178
Size: 30 165 30 2245 30 292 30 4295
Fragments arranged by size:
4295 2245 292 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2238 2654 3137 3140 3311 1186
Size: 1052 416 483 3 171 4992
Fragments arranged by size:
4992 1052 483 416 171 3
BseYI C'CCAG_C
Cuts at: 2227 2499 6320 6816 2227
Size: 272 3821 496 2528
Fragments arranged by size:
3821 2528 496 272
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2067 2258 2417 1163
Size: 573 331 191 159 5863
Fragments arranged by size:
5863 573 331 191 159
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4567 5328 6356 1540
Size: 275 186 2566 761 1028 2301
Fragments arranged by size:
2566 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3846 5178 5263 6334 3846
Size: 1332 85 1071 4629
Fragments arranged by size:
4629 1332 1071 85
BsmI GAATG_Cn'
Cuts at: 4032 4628 4721 4032
Size: 596 93 6428
Fragments arranged by size:
6428 596 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3709 3709
Size: 7117
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2271 2384 2816 3846 4436 5178 5263
Size: 399 481 113 432 1030 590 742 85
Cuts at: 5263 6334 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 1030 742 590 481 432 399
113 85
BspEI T'CCGG_A
Cuts at: 3531 6780 3531
Size: 3249 3868
BspHI T'CATG_A
Cuts at: 3763 5006 6736 3763
Size: 1243 1730 4144
Fragments arranged by size:
4144 1730 1243
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4472 844
Size: 3628 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2029 5004 1394
Size: 429 206 2975 3507
Fragments arranged by size:
3507 2975 429 206
BsrDI GCAATG_nn'
Cuts at: 65 1248 2985 4027 4038 5612 5786 65
Size: 1183 1737 1042 11 1574 174 1396
Fragments arranged by size:
1737 1574 1396 1183 1042 174 11
BsrFI r'CCGG_y
Cuts at: 1700 1810 2510 5761 5948 1700
Size: 110 700 3251 187 2869
Fragments arranged by size:
3251 2869 700 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7117
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7117
BssSI C'ACGA_G
Cuts at: 5177 6189 5177
Size: 1012 6105
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7117
BstBI TT'CG_AA
Cuts at: 5921 5921
Size: 7117
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7117
BstXI CCAn_nnnn'nTGG
Cuts at: 3242 4368 3242
Size: 1126 5991
BstYI r'GATC_y
Cuts at: 1972 2078 2428 3088 3547 4066 4447 4814 5199
Size: 106 350 660 459 519 381 367 385
Cuts at: 5199 5216 6668 6754 6766 7112 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 660 519 459 385 381 367
350 346 106 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3552 3552
Size: 7117
BtgI C'CryG_G
Cuts at: 513 1065 2004 3542 4246 513
Size: 552 939 1538 704 3384
Fragments arranged by size:
3384 1538 939 704 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2153 487
Size: 683 56 927 5451
Fragments arranged by size:
5451 927 683 56
BtsI GCAGTG_nn'
Cuts at: 2640 3900 4633 5427 5447 2640
Size: 1260 733 794 20 4310
Fragments arranged by size:
4310 1260 794 733 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7117
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7082
DraI TTT'AAA
Cuts at: 2937 3628 4421 2937
Size: 691 793 5633
Fragments arranged by size:
5633 793 691
DraIII CAC_nnn'GTG
Cuts at: 2440 2440
Size: 7117
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4079 6124 809
Size: 507 2763 2045 1802
Fragments arranged by size:
2763 2045 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4249 4564 5453
Size: 54 1425 50 275 18 2419 315 889
Cuts at: 5453 7082 8
Size: 1629 43
Fragments arranged by size:
2419 1629 1425 889 315 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4564 1537
Size: 275 2752 4090
Fragments arranged by size:
4090 2752 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2213 2790 3321 3441 3834 5047 1353
Size: 860 577 531 120 393 1213 3423
Fragments arranged by size:
3423 1213 860 577 531 393 120
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2073 5704 6078 6224 387
Size: 1064 138 484 3631 374 146 1280
Fragments arranged by size:
3631 1280 1064 484 374 146 138
EcoICRI GAG'CTC
Cuts at: 3844 3844
Size: 7117
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2615 3706 3714 4234 5180 5777 6564
Size: 393 983 1091 8 520 946 597 787
Cuts at: 6564 6832 1239
Size: 268 1524
Fragments arranged by size:
1524 1091 983 946 787 597 520 393
268 8
EcoNI CCTnn'n_nnAGG
Cuts at: 2294 2416 3038 2294
Size: 122 622 6373
Fragments arranged by size:
6373 622 122
EcoO109I rG'GnC_Cy
Cuts at: 2545 2965 3181 2545
Size: 420 216 6481
Fragments arranged by size:
6481 420 216
EcoRI G'AATT_C
Cuts at: 3495 4426 4804 3495
Size: 931 378 5808
Fragments arranged by size:
5808 931 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3041 3166 3041
Size: 125 6992
FspI TGC'GCA
Cuts at: 5623 5623
Size: 7117
HaeII r_GCGC'y
Cuts at: 1678 2647 3871 6264 1678
Size: 969 1224 2393 2531
Fragments arranged by size:
2531 2393 1224 969
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3287 3319 4093 4125 4209
Size: 32 135 32 1715 32 774 32 84
Cuts at: 4209 4241 4327 4359 5758 5790 5832 5864 1373
Size: 32 86 32 1399 32 42 32 2626
Fragments arranged by size:
2626 1715 1399 774 135 86 84 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2158 4461 4707 669
Size: 988 281 220 2303 246 3079
Fragments arranged by size:
3079 2303 988 281 246 220
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7117
HpaI GTT'AAC
Cuts at: 4707 4707
Size: 7117
KpnI G_GTAC'C
Cuts at: 4245 4442 4245
Size: 197 6920
MfeI C'AATT_G
Cuts at: 4716 4716
Size: 7117
MluI A'CGCG_T
Cuts at: 6993 6993
Size: 7117
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2576 2860 3399 6231 6415 1305
Size: 1271 284 539 2832 184 2007
Fragments arranged by size:
2832 2007 1271 539 284 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4251 10
Size: 54 1425 343 2419 2876
Fragments arranged by size:
2876 2419 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2343 2978 3541 5075 5434 5593 518
Size: 648 1177 635 563 1534 359 159 2042
Fragments arranged by size:
2042 1534 1177 648 635 563 359 159
MspA1I CmG'CkG
Cuts at: 1606 2089 2227 2308 3908 4533 5210 6358 6603
Size: 483 138 81 1600 625 677 1148 245
Cuts at: 6603 1606
Size: 2120
Fragments arranged by size:
2120 1600 1148 677 625 483 245 138
81
NcoI C'CATG_G
Cuts at: 513 1065 2004 3542 4246 513
Size: 552 939 1538 704 3384
Fragments arranged by size:
3384 1538 939 704 552
NdeI CA'TA_TG
Cuts at: 387 4348 387
Size: 3961 3156
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7117
NotI GC'GGCC_GC
Cuts at: 4564 4564
Size: 7117
NspI r_CATG'y
Cuts at: 1132 1229 4153 4475 6020 1132
Size: 97 2924 322 1545 2229
Fragments arranged by size:
2924 2229 1545 322 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7117
PciI A'CATG_T
Cuts at: 4149 6016 4149
Size: 1867 5250
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7117
PmeI GTTT'AAAC
Cuts at: 4421 4421
Size: 7117
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5186 5218 6699 6731 5186
Size: 32 1481 32 5572
Fragments arranged by size:
5572 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2965 3181 2965
Size: 216 6901
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7117
PsiI TTA'TAA
Cuts at: 4687 4687
Size: 7117
PstI C_TGCA'G
Cuts at: 830 3907 4469 830
Size: 3077 562 3478
Fragments arranged by size:
3478 3077 562
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7117
PvuII CAG'CTG
Cuts at: 2227 2308 2227
Size: 81 7036
SacI G_AGCT'C
Cuts at: 3846 3846
Size: 7117
SalI G'TCGA_C
Cuts at: 1936 2156 4459 1936
Size: 220 2303 4594
Fragments arranged by size:
4594 2303 220
SapI GCTCTTCn'nnn_
Cuts at: 3834 3834
Size: 7117
SbfI CC_TGCA'GG
Cuts at: 4469 4469
Size: 7117
ScaI AGT'ACT
Cuts at: 4299 5365 7077 4299
Size: 1066 1712 4339
Fragments arranged by size:
4339 1712 1066
SfcI C'TryA_G
Cuts at: 826 1045 3003 3903 4044 4465 5600 6281 6472
Size: 219 1958 900 141 421 1135 681 191
Cuts at: 6472 826
Size: 1471
Fragments arranged by size:
1958 1471 1135 900 681 421 219 191
141
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7117
SgrAI Cr'CCGG_yG
Cuts at: 1700 2510 1700
Size: 810 6307
SmaI CCC'GGG
Cuts at: 1872 4444 1872
Size: 2572 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2030 3521 3567 4287 4401 5221
Size: 197 926 87 1491 46 720 114 820
Cuts at: 5221 6122 6384 820
Size: 901 262 1553
Fragments arranged by size:
1553 1491 926 901 820 720 262 197
114 87 46
SnaBI TAC'GTA
Cuts at: 493 4908 493
Size: 4415 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7117
SphI G_CATG'C
Cuts at: 1132 4475 1132
Size: 3343 3774
SspI AAT'ATT
Cuts at: 5 52 3585 5041 5
Size: 47 3533 1456 2081
Fragments arranged by size:
3533 2081 1456 47
StuI AGG'CCT
Cuts at: 1517 3519 1517
Size: 2002 5115
StyI C'CwwG_G
Cuts at: 513 1065 2004 3542 3834 4246 4556 513
Size: 552 939 1538 292 412 310 3074
Fragments arranged by size:
3074 1538 939 552 412 310 292
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2219 2375 5144 5161 5314 5499 5944
Size: 1255 639 156 2769 17 153 185 445
Cuts at: 5944 325
Size: 1498
Fragments arranged by size:
2769 1498 1255 639 445 185 156 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2335 4297 5363
Size: 274 80 33 51 1443 358 1962 1066
Cuts at: 5363 7075 96
Size: 1712 138
Fragments arranged by size:
1962 1712 1443 1066 358 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2553 2585 3970 4002 4160 4192 7077 7109 2553
Size: 32 1385 32 158 32 2885 32 2561
Fragments arranged by size:
2885 2561 1385 158 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7117
XbaI T'CTAG_A
Cuts at: 4453 4453
Size: 7117
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2496 2579 2496
Size: 83 7034
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7117
XmaI C'CCGG_G
Cuts at: 1870 4442 1870
Size: 2572 4545
XmnI GAAnn'nnTTC
Cuts at: 3561 5246 3561
Size: 1685 5432
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4925 276
Size: 53 83 186 936 3391 2468
Fragments arranged by size:
3391 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstZ17I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI
SrfI SwaI