Restriction map for
pFN21AA1398
(Circular) (Six-base) MAPSORT of: pFN21AA1398.seq Check: 4019 from: 1 to: 9385
pFN21AA1398 9385 bp
With 182 enzymes: SgfI *
July 4, 2020 18:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5905 5905
Size: 9385
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5379 7195 278
Size: 53 83 186 936 3843 1816 2468
Fragments arranged by size:
3843 2468 1816 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2090 6728 1937
Size: 153 4638 4594
Fragments arranged by size:
4638 4594 153
Acc65I G'GTAC_C
Cuts at: 6706 6706
Size: 9385
AclI AA'CG_TT
Cuts at: 1563 7512 7885 1563
Size: 5949 373 3063
Fragments arranged by size:
5949 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2661 4787 5765 6344 6459 6521 6596 7448
Size: 1029 2126 978 579 115 62 75 852
Cuts at: 7448 8832 9100 1632
Size: 1384 268 1917
Fragments arranged by size:
2126 1917 1384 1029 978 852 579 268
115 75 62
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9188
AflIII A'CryG_T
Cuts at: 1203 6045 8284 9261 1203
Size: 4842 2239 977 1327
Fragments arranged by size:
4842 2239 1327 977
AgeI A'CCGG_T
Cuts at: 8216 8216
Size: 9385
AhdI GACnn_n'nnGTC
Cuts at: 8114 8114
Size: 9385
AleI CACnn'nnGTG
Cuts at: 1166 4724 1166
Size: 3558 5827
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9385
AlwNI CAG_nnn'CTG
Cuts at: 2585 4265 4780 4985 5053 7128 8700 2585
Size: 1680 515 205 68 2075 1572 3270
Fragments arranged by size:
3270 2075 1680 1572 515 205 68
ApaI G_GGCC'C
Cuts at: 2800 2860 2920 2950 2980 3010 3040 3070 3100
Size: 60 60 30 30 30 30 30 30
Cuts at: 3100 3190 3250 3280 3310 3340 3370 3400 3430
Size: 90 60 30 30 30 30 30 30
Cuts at: 3430 3460 3490 3610 3640 3700 3730 3760 3790
Size: 30 30 120 30 60 30 30 30
Cuts at: 3790 3820 3880 3910 4371 6274 2800
Size: 30 60 30 461 1903 5911
Fragments arranged by size:
5911 1903 461 120 90 60 60 60
60 60 30 30 30 30 30 30
30 30 30 30 30 30 30 30
30 30 30 30 30 30
ApaLI G'TGCA_C
Cuts at: 7442 8598 7442
Size: 1156 8229
ApoI r'AATT_y
Cuts at: 1493 2476 6694 6920 7072 9181 1493
Size: 983 4218 226 152 2109 1697
Fragments arranged by size:
4218 2109 1697 983 226 152
AscI GG'CGCG_CC
Cuts at: 6547 6547
Size: 9385
AseI AT'TA_AT
Cuts at: 160 1032 7939 160
Size: 872 6907 1606
Fragments arranged by size:
6907 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9385
AvaI C'yCGr_G
Cuts at: 1870 1943 4704 5964 6710 1870
Size: 73 2761 1260 746 4545
Fragments arranged by size:
4545 2761 1260 746 73
BamHI G'GATC_C
Cuts at: 6715 7082 6715
Size: 367 9018
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2533 2548 2561 3215 4306
Size: 325 217 627 746 15 13 654 1091
Cuts at: 4306 5936 6292 6674 6706 8161 618
Size: 1630 356 382 32 1455 1842
Fragments arranged by size:
1842 1630 1455 1091 746 654 627 382
356 325 217 32 15 13
BanII G_rGCy'C
Cuts at: 1391 2311 2650 2800 2860 2890 2920 2950 2980
Size: 920 339 150 60 30 30 30 30
Cuts at: 2980 3010 3040 3070 3100 3130 3160 3190 3250
Size: 30 30 30 30 30 30 30 60
Cuts at: 3250 3280 3310 3340 3370 3400 3430 3460 3490
Size: 30 30 30 30 30 30 30 30
Cuts at: 3490 3610 3640 3700 3730 3760 3790 3820 3880
Size: 120 30 60 30 30 30 30 60
Cuts at: 3880 3910 3940 3970 4000 4030 4084 4301 4371
Size: 30 30 30 30 30 54 217 70
Cuts at: 4371 4632 4947 5472 5772 5955 6274 6704 1391
Size: 261 315 525 300 183 319 430 4072
Fragments arranged by size:
4072 920 525 430 339 319 315 300
261 217 183 150 120 70 60 60
60 60 54 30 30 30 30 30
30 30 30 30 30 30 30 30
30 30 30 30 30 30 30 30
30 30 30 30 30 30 30 30
BbeI G_GCGC'C
Cuts at: 5940 6296 5940
Size: 356 9029
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 6332 6465 6584 928
Size: 971 4433 133 119 3729
Fragments arranged by size:
4433 3729 971 133 119
BbvCI CC'TCA_GC
Cuts at: 3120 3210 3900 6131 3120
Size: 90 690 2231 6374
Fragments arranged by size:
6374 2231 690 90
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5396 5430 6188 6222 6664 6698 7576
Size: 34 3459 34 758 34 442 34 878
Cuts at: 7576 7610 1903
Size: 34 3678
Fragments arranged by size:
3678 3459 878 758 442 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7277 8487 7277
Size: 1210 8175
BclI T'GATC_A
Cuts at: 1548 2257 1548
Size: 709 8676
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2885 3005 3035
Size: 107 122 71 631 605 1213 120 30
Cuts at: 3035 3065 3095 3155 3245 3275 3335 3365 3395
Size: 30 30 60 90 30 60 30 30
Cuts at: 3395 3425 3455 3485 3605 3635 3695 3725 3755
Size: 30 30 30 120 30 60 30 30
Cuts at: 3755 3785 3815 3935 4676 5174 5390 5540 5561
Size: 30 30 120 741 498 216 150 21
Cuts at: 5561 5657 7996 136
Size: 96 2339 1525
Fragments arranged by size:
2339 1525 1213 741 631 605 498 216
150 122 120 120 120 107 96 90
71 60 60 60 30 30 30 30
30 30 30 30 30 30 30 30
30 30 21
BglII A'GATC_T
Cuts at: 9380 9380
Size: 9385
BlpI GC'TnA_GC
Cuts at: 4946 6802 4946
Size: 1856 7529
Bme1580I G_kGCm'C
Cuts at: 1790 2553 2800 2860 2920 2950 2980 3010 3040
Size: 763 247 60 60 30 30 30 30
Cuts at: 3040 3070 3100 3190 3220 3250 3280 3310 3340
Size: 30 30 90 30 30 30 30 30
Cuts at: 3340 3370 3400 3430 3460 3490 3610 3640 3700
Size: 30 30 30 30 30 120 30 60
Cuts at: 3700 3730 3760 3790 3820 3880 3910 4127 4371
Size: 30 30 30 30 60 30 217 244
Cuts at: 4371 6274 6677 7446 8602 1790
Size: 1903 403 769 1156 2573
Fragments arranged by size:
2573 1903 1156 769 763 403 247 244
217 120 90 60 60 60 60 30
30 30 30 30 30 30 30 30
30 30 30 30 30 30 30 30
30 30 30 30 30
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9385
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2330 2549 4170
Size: 416 53 83 399 24 913 219 1621
Cuts at: 4170 7116 8074 442
Size: 2946 958 1753
Fragments arranged by size:
2946 1753 1621 958 913 416 399 219
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9385
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5791 6508 6694 5791
Size: 717 186 8482
Fragments arranged by size:
8482 717 186
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4835 5267 5366 5450 5471 5939 6356 6377
Size: 3596 432 99 84 21 468 417 21
Cuts at: 6377 8045 1239
Size: 1668 2579
Fragments arranged by size:
3596 2579 1668 468 432 417 99 84
21 21
Bpu10I CC'TnA_GC
Cuts at: 3120 3210 3900 4029 5471 6131 3120
Size: 90 690 129 1442 660 6374
Fragments arranged by size:
6374 1442 690 660 129 90
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5405 7510 8375 8673 5405
Size: 2105 865 298 6117
Fragments arranged by size:
6117 2105 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4420 5877 8048 882
Size: 617 2921 1457 2171 2219
Fragments arranged by size:
2921 2219 2171 1457 617
BsaAI yAC'GTr
Cuts at: 493 1204 4762 6247 7176 493
Size: 711 3558 1485 929 2702
Fragments arranged by size:
3558 2702 1485 929 711
BsaBI GATnn'nnATC
Cuts at: 2262 9379 2262
Size: 7117 2268
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5066 5376 5937 6293
Size: 53 83 186 936 3533 310 561 356
Cuts at: 6293 7192 7574 8209 275
Size: 899 382 635 1451
Fragments arranged by size:
3533 1451 936 899 635 561 382 356
310 186 83 53
BsaWI w'CCGG_w
Cuts at: 4794 7818 8216 8490 8637 9048 4794
Size: 3024 398 274 147 411 5131
Fragments arranged by size:
5131 3024 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 5417 5447 5771 5801 5903
Size: 30 165 30 4014 30 324 30 102
Cuts at: 5903 5933 6032 6062 1178
Size: 30 99 30 4501
Fragments arranged by size:
4501 4014 324 165 102 99 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2395 5099 5270 5764 5822 5918 5921 5924
Size: 1209 2704 171 494 58 96 3 3
Cuts at: 5924 5960 5963 6416 6500 1186
Size: 36 3 453 84 4071
Fragments arranged by size:
4071 2704 1209 494 453 171 96 84
58 36 3 3 3
BseYI C'CCAG_C
Cuts at: 4455 5307 6266 6274 6298 8588 9084 4455
Size: 852 959 8 24 2290 496 4756
Fragments arranged by size:
4756 2290 959 852 496 24 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2360 4748 4793 5192 5564 1163
Size: 573 624 2388 45 399 372 4984
Fragments arranged by size:
4984 2388 624 573 399 372 45
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5556 6087 6393 6835 7596 8624
Size: 275 186 3555 531 306 442 761 1028
Cuts at: 8624 1540
Size: 2301
Fragments arranged by size:
3555 2301 1028 761 531 442 306 275
186
BsiHKAI G_wGCw'C
Cuts at: 3850 4084 4632 4761 4947 5472 5822 5955 6045
Size: 234 548 129 186 525 350 133 90
Cuts at: 6045 7446 7531 8602 3850
Size: 1401 85 1071 4633
Fragments arranged by size:
4633 1401 1071 548 525 350 234 186
133 129 90 85
BsmI GAATG_Cn'
Cuts at: 6896 6989 6896
Size: 93 9292
BsmBI CGTCTCn'nnnn_
Cuts at: 5804 5858 5804
Size: 54 9331
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2311 2553 2650 2800 2860 2890 2920
Size: 399 521 242 97 150 60 30 30
Cuts at: 2920 2950 2980 3010 3040 3070 3100 3130 3160
Size: 30 30 30 30 30 30 30 30
Cuts at: 3160 3190 3220 3250 3280 3310 3340 3370 3400
Size: 30 30 30 30 30 30 30 30
Cuts at: 3400 3430 3460 3490 3610 3640 3700 3730 3760
Size: 30 30 30 120 30 60 30 30
Cuts at: 3760 3790 3820 3850 3880 3910 3940 3970 4000
Size: 30 30 30 30 30 30 30 30
Cuts at: 4000 4030 4084 4127 4301 4371 4632 4761 4947
Size: 30 54 43 174 70 261 129 186
Cuts at: 4947 5472 5772 5822 5955 6045 6274 6677 6704
Size: 525 300 50 133 90 229 403 27
Cuts at: 6704 7446 7531 8602 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 525 521 403 399 300
261 242 229 186 174 150 133 129
120 97 90 85 70 60 60 54
50 43 30 30 30 30 30 30
30 30 30 30 30 30 30 30
30 30 30 30 30 30 30 30
30 30 30 30 30 30 30 30
30 30 30 27
BspEI T'CCGG_A
Cuts at: 4794 9048 4794
Size: 4254 5131
BspHI T'CATG_A
Cuts at: 2260 7274 9004 2260
Size: 5014 1730 2641
Fragments arranged by size:
5014 2641 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5038 5895 5905 6740 844
Size: 4194 857 10 835 3489
Fragments arranged by size:
4194 3489 857 835 10
BsrBI CCG'CTC
Cuts at: 1394 1823 7272 1394
Size: 429 5449 3507
Fragments arranged by size:
5449 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 7880 8054 65
Size: 1183 6632 174 1396
Fragments arranged by size:
6632 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6458 8029 8216 1700
Size: 110 4648 1571 187 2869
Fragments arranged by size:
4648 2869 1571 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9385
BssHII G'CGCG_C
Cuts at: 1925 6547 1925
Size: 4622 4763
BssSI C'ACGA_G
Cuts at: 4891 6556 7445 8457 4891
Size: 1665 889 1012 5819
Fragments arranged by size:
5819 1665 1012 889
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4780 5053 5250 6049 794
Size: 3986 273 197 799 4130
Fragments arranged by size:
4130 3986 799 273 197
BstBI TT'CG_AA
Cuts at: 2498 8189 2498
Size: 5691 3694
BstEII G'GTnAC_C
Cuts at: 1184 4529 1184
Size: 3345 6040
BstXI CCAn_nnnn'nTGG
Cuts at: 6121 6121
Size: 9385
BstYI r'GATC_y
Cuts at: 1972 4470 4791 5716 6715 7082 7467 7484 8936
Size: 2498 321 925 999 367 385 17 1452
Cuts at: 8936 9022 9034 9380 1972
Size: 86 12 346 1977
Fragments arranged by size:
2498 1977 1452 999 925 385 367 346
321 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 4419 5272 5535 5550 5643 5763
Size: 552 939 2415 853 263 15 93 120
Cuts at: 5763 513
Size: 4135
Fragments arranged by size:
4135 2415 939 853 552 263 120 93
15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 6213 487
Size: 683 56 4987 3659
Fragments arranged by size:
4987 3659 683 56
BtsI GCAGTG_nn'
Cuts at: 5822 6901 7695 7715 5822
Size: 1079 794 20 7492
Fragments arranged by size:
7492 1079 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9385
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9350
DraI TTT'AAA
Cuts at: 6689 6689
Size: 9385
DraIII CAC_nnn'GTG
Cuts at: 5706 5706
Size: 9385
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8392 809
Size: 507 7076 1802
Fragments arranged by size:
7076 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4581 4926 5199
Size: 54 1425 50 275 18 2751 345 273
Cuts at: 5199 5547 5553 6084 6390 6832 7721 9350 8
Size: 348 6 531 306 442 889 1629 43
Fragments arranged by size:
2751 1629 1425 889 531 442 348 345
306 275 273 54 50 43 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 5553 6084 6390 6832 1537
Size: 275 3741 531 306 442 4090
Fragments arranged by size:
4090 3741 531 442 306 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2226 4557 4806 5888 5982 6233 7315 1353
Size: 873 2331 249 1082 94 251 1082 3423
Fragments arranged by size:
3423 2331 1082 1082 873 251 249 94
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2624 2804 5177 5249 5312 5408
Size: 1064 138 1035 180 2373 72 63 96
Cuts at: 5408 5876 7972 8346 8492 387
Size: 468 2096 374 146 1280
Fragments arranged by size:
2373 2096 1280 1064 1035 468 374 180
146 138 96 72 63
EcoICRI GAG'CTC
Cuts at: 4082 4630 4945 5470 5953 4082
Size: 548 315 525 483 7514
Fragments arranged by size:
7514 548 525 483 315
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2661 4787 4835 5267 5366 5450 5471
Size: 393 1029 2126 48 432 99 84 21
Cuts at: 5471 5765 5939 6344 6356 6377 6459 6521 6596
Size: 294 174 405 12 21 82 62 75
Cuts at: 6596 7448 8045 8832 9100 1239
Size: 852 597 787 268 1524
Fragments arranged by size:
2126 1524 1029 852 787 597 432 405
393 294 268 174 99 84 82 75
62 48 21 21 12
EcoNI CCTnn'n_nnAGG
Cuts at: 4850 5108 5345 5591 4850
Size: 258 237 246 8644
Fragments arranged by size:
8644 258 246 237
EcoO109I rG'GnC_Cy
Cuts at: 2796 2856 2857 2916 2946 2976 3006 3036 3066
Size: 60 1 59 30 30 30 30 30
Cuts at: 3066 3096 3186 3246 3276 3306 3336 3366 3396
Size: 30 90 60 30 30 30 30 30
Cuts at: 3396 3426 3456 3457 3486 3487 3516 3546 3576
Size: 30 30 1 29 1 29 30 30
Cuts at: 3576 3606 3636 3666 3696 3726 3756 3786 3816
Size: 30 30 30 30 30 30 30 30
Cuts at: 3816 3876 3906 4170 4200 4368 5050 5089 5338
Size: 60 30 264 30 168 682 39 249
Cuts at: 5338 5650 5732 6270 6271 6310 2796
Size: 312 82 538 1 39 5871
Fragments arranged by size:
5871 682 538 312 264 249 168 90
82 60 60 60 59 39 39 30
30 30 30 30 30 30 30 30
30 30 30 30 30 30 30 30
30 30 30 30 30 30 30 30
29 29 1 1 1 1
EcoRI G'AATT_C
Cuts at: 2476 6694 7072 2476
Size: 4218 378 4789
Fragments arranged by size:
4789 4218 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 6032 6032
Size: 9385
FspI TGC'GCA
Cuts at: 5243 7891 5243
Size: 2648 6737
HaeII r_GCGC'y
Cuts at: 1678 5104 5940 6096 6296 8532 1678
Size: 3426 836 156 200 2236 2531
Fragments arranged by size:
3426 2531 2236 836 200 156
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2242 2274 2608 2640 4071
Size: 32 135 32 670 32 334 32 1431
Cuts at: 4071 4103 4460 4492 4783 4815 5417 5449 5771
Size: 32 357 32 291 32 602 32 322
Cuts at: 5771 5803 8026 8058 8100 8132 1373
Size: 32 2223 32 42 32 2626
Fragments arranged by size:
2626 2223 1431 670 602 357 334 322
291 135 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2091 6531 6729 6975 669
Size: 988 281 153 4440 198 246 3079
Fragments arranged by size:
4440 3079 988 281 246 198 153
HindIII A'AGCT_T
Cuts at: 748 4931 748
Size: 4183 5202
HpaI GTT'AAC
Cuts at: 6531 6975 6531
Size: 444 8941
KasI G'GCGC_C
Cuts at: 5936 6292 5936
Size: 356 9029
KpnI G_GTAC'C
Cuts at: 6710 6710
Size: 9385
MfeI C'AATT_G
Cuts at: 6984 6984
Size: 9385
MluI A'CGCG_T
Cuts at: 9261 9261
Size: 9385
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2354 8499 8683 1305
Size: 1049 6145 184 2007
Fragments arranged by size:
6145 2007 1049 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4583 4928 5549 10
Size: 54 1425 343 2751 345 621 3846
Fragments arranged by size:
3846 2751 1425 621 345 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4724 7343 7702 7861 518
Size: 648 3558 2619 359 159 2042
Fragments arranged by size:
3558 2619 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2459 4232 4459 4558 4696 4780 4813 4837
Size: 853 1773 227 99 138 84 33 24
Cuts at: 4837 5008 5033 5059 5765 6322 6334 6376 6499
Size: 171 25 26 706 557 12 42 123
Cuts at: 6499 6595 6637 6801 7478 8626 8871 1606
Size: 96 42 164 677 1148 245 2120
Fragments arranged by size:
2120 1773 1148 853 706 677 557 245
227 171 164 138 123 99 96 84
42 42 33 26 25 24 12
NaeI GCC'GGC
Cuts at: 6460 6460
Size: 9385
NarI GG'CG_CC
Cuts at: 5937 6293 5937
Size: 356 9029
NcoI C'CATG_G
Cuts at: 513 1065 2004 5535 5643 513
Size: 552 939 3531 108 4255
Fragments arranged by size:
4255 3531 939 552 108
NdeI CA'TA_TG
Cuts at: 387 2116 387
Size: 1729 7656
NgoMIV G'CCGG_C
Cuts at: 6458 6458
Size: 9385
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9385
NotI GC'GGCC_GC
Cuts at: 6084 6832 6084
Size: 748 8637
NspI r_CATG'y
Cuts at: 1132 1229 4741 5878 6423 6743 8288 1132
Size: 97 3512 1137 545 320 1545 2229
Fragments arranged by size:
3512 2229 1545 1137 545 320 97
PasI CC'CwG_GG
Cuts at: 1348 2866 1348
Size: 1518 7867
PciI A'CATG_T
Cuts at: 8284 8284
Size: 9385
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2585 1108
Size: 1477 7908
PfoI T'CCnGG_A
Cuts at: 5283 5514 6354 5283
Size: 231 840 8314
Fragments arranged by size:
8314 840 231
PmeI GTTT'AAAC
Cuts at: 6689 6689
Size: 9385
PmlI CAC'GTG
Cuts at: 4762 4762
Size: 9385
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5311 5343 7454 7486 8967 8999 5311
Size: 32 2111 32 1481 32 5697
Fragments arranged by size:
5697 2111 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3516 3546 3576 3666 4170 4200 5050 6310 3516
Size: 30 30 90 504 30 850 1260 6591
Fragments arranged by size:
6591 1260 850 504 90 30 30 30
PshAI GACnn'nnGTC
Cuts at: 1613 4412 1613
Size: 2799 6586
PsiI TTA'TAA
Cuts at: 6955 6955
Size: 9385
PspOMI G'GGCC_C
Cuts at: 2796 2856 2916 2946 2976 3006 3036 3066 3096
Size: 60 60 30 30 30 30 30 30
Cuts at: 3096 3186 3246 3276 3306 3336 3366 3396 3426
Size: 90 60 30 30 30 30 30 30
Cuts at: 3426 3456 3486 3606 3636 3696 3726 3756 3786
Size: 30 30 120 30 60 30 30 30
Cuts at: 3786 3816 3876 3906 4367 6270 2796
Size: 30 60 30 461 1903 5911
Fragments arranged by size:
5911 1903 461 120 90 60 60 60
60 60 30 30 30 30 30 30
30 30 30 30 30 30 30 30
30 30 30 30 30 30
PstI C_TGCA'G
Cuts at: 830 4785 4851 5058 5064 5097 5715 5829 5892
Size: 3955 66 207 6 33 618 114 63
Cuts at: 5892 6567 6630 6737 830
Size: 675 63 107 3478
Fragments arranged by size:
3955 3478 675 618 207 114 107 66
63 63 33 6
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9385
PvuII CAG'CTG
Cuts at: 2459 4232 4558 4696 4780 4813 4837 5033 5059
Size: 1773 326 138 84 33 24 196 26
Cuts at: 5059 6322 6334 6499 6595 6637 2459
Size: 1263 12 165 96 42 5207
Fragments arranged by size:
5207 1773 1263 326 196 165 138 96
84 42 33 26 24 12
SacI G_AGCT'C
Cuts at: 4084 4632 4947 5472 5955 4084
Size: 548 315 525 483 7514
Fragments arranged by size:
7514 548 525 483 315
SacII CC_GC'GG
Cuts at: 5766 5766
Size: 9385
SalI G'TCGA_C
Cuts at: 1936 2089 6727 1936
Size: 153 4638 4594
Fragments arranged by size:
4638 4594 153
SanDI GG'GwC_CC
Cuts at: 3516 3546 3576 3666 4170 4200 3516
Size: 30 30 90 504 30 8701
Fragments arranged by size:
8701 504 90 30 30 30
SapI GCTCTTCn'nnn_
Cuts at: 5888 6233 5888
Size: 345 9040
SbfI CC_TGCA'GG
Cuts at: 6737 6737
Size: 9385
ScaI AGT'ACT
Cuts at: 7633 9345 7633
Size: 1712 7673
SexAI A'CCwGG_T
Cuts at: 6030 6030
Size: 9385
SfcI C'TryA_G
Cuts at: 826 1045 2469 4781 4847 5054 5060 5093 5711
Size: 219 1424 2312 66 207 6 33 618
Cuts at: 5711 5825 5888 6563 6626 6733 7868 8549 8740
Size: 114 63 675 63 107 1135 681 191
Cuts at: 8740 826
Size: 1471
Fragments arranged by size:
2312 1471 1424 1135 681 675 618 219
207 191 114 107 66 63 63 33
6
SfiI GGCCn_nnn'nGGCC
Cuts at: 3005 3035 3065 3095 3245 3275 3335 3365 3395
Size: 30 30 30 150 30 60 30 30
Cuts at: 3395 3425 3455 3485 3605 3635 3695 3725 3755
Size: 30 30 30 120 30 60 30 30
Cuts at: 3755 3785 3815 5561 3005
Size: 30 30 1746 6829
Fragments arranged by size:
6829 1746 150 120 60 60 30 30
30 30 30 30 30 30 30 30
30 30 30 30
SfoI GGC'GCC
Cuts at: 5938 6294 5938
Size: 356 9029
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9385
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9385
SmaI CCC'GGG
Cuts at: 1872 4706 5966 6712 1872
Size: 2834 1260 746 4545
Fragments arranged by size:
4545 2834 1260 746
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5420 7489 8390 8652 820
Size: 197 926 3477 2069 901 262 1553
Fragments arranged by size:
3477 2069 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 7176 493
Size: 6683 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9385
SphI G_CATG'C
Cuts at: 1132 4741 5878 6743 1132
Size: 3609 1137 865 3774
Fragments arranged by size:
3774 3609 1137 865
SspI AAT'ATT
Cuts at: 5 52 7309 5
Size: 47 7257 2081
Fragments arranged by size:
7257 2081 47
StuI AGG'CCT
Cuts at: 1517 5564 6491 1517
Size: 4047 927 4411
Fragments arranged by size:
4411 4047 927
StyI C'CwwG_G
Cuts at: 513 1065 2004 2028 2412 2526 2565 2716 2746
Size: 552 939 24 384 114 39 151 30
Cuts at: 2746 2776 4060 4210 5025 5535 5598 5643 5928
Size: 30 1284 150 815 510 63 45 285
Cuts at: 5928 6114 6824 513
Size: 186 710 3074
Fragments arranged by size:
3074 1284 939 815 710 552 510 384
285 186 151 150 114 63 45 39
30 30 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2549 7412 7429 7582 7767 8212 325
Size: 1255 969 4863 17 153 185 445 1498
Fragments arranged by size:
4863 1498 1255 969 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 7631 9343 96
Size: 274 80 33 51 1443 5654 1712 138
Fragments arranged by size:
5654 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5903 5935 9345 9377 5903
Size: 32 3410 32 5911
Fragments arranged by size:
5911 3410 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4255 5111 1316
Size: 2939 856 5590
Fragments arranged by size:
5590 2939 856
XbaI T'CTAG_A
Cuts at: 6721 6721
Size: 9385
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4834 4897 5542 6251 4834
Size: 63 645 709 7968
Fragments arranged by size:
7968 709 645 63
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9385
XmaI C'CCGG_G
Cuts at: 1870 4704 5964 6710 1870
Size: 2834 1260 746 4545
Fragments arranged by size:
4545 2834 1260 746
XmnI GAAnn'nnTTC
Cuts at: 5326 7514 5326
Size: 2188 7197
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5377 7193 276
Size: 53 83 186 936 3843 1816 2468
Fragments arranged by size:
3843 2468 1816 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I
EcoRV FseI FspAI NruI NsiI PacI PspXI PsrI
RsrII SrfI SwaI