Restriction map for
pFN21AA1395
(Circular) (Six-base) MAPSORT of: pFN21AA1395.seq Check: 7510 from: 1 to: 10846
pFN21AA1395 10846 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2065 3909 4848 5062 6585 6930 2065
Size: 1844 939 214 1523 345 5981
Fragments arranged by size:
5981 1844 1523 939 345 214
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5854 7398 8656 278
Size: 53 83 186 936 4318 1544 1258 2468
Fragments arranged by size:
4318 2468 1544 1258 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3825 7122 8189 1937
Size: 1888 3297 1067 4594
Fragments arranged by size:
4594 3297 1888 1067
Acc65I G'GTAC_C
Cuts at: 5627 5984 8167 5627
Size: 357 2183 8306
Fragments arranged by size:
8306 2183 357
AclI AA'CG_TT
Cuts at: 1563 8973 9346 1563
Size: 7410 373 3063
Fragments arranged by size:
7410 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2169 3026 3667 5029 5400 6275 6547 7790
Size: 537 857 641 1362 371 875 272 1243
Cuts at: 7790 8909 10293 10561 1632
Size: 1119 1384 268 1917
Fragments arranged by size:
1917 1384 1362 1243 1119 875 857 641
537 371 272 268
AfeI AGC'GCT
Cuts at: 3226 5816 3226
Size: 2590 8256
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 10649
AflIII A'CryG_T
Cuts at: 1203 3660 9745 10722 1203
Size: 2457 6085 977 1327
Fragments arranged by size:
6085 2457 1327 977
AgeI A'CCGG_T
Cuts at: 3817 9677 3817
Size: 5860 4986
AhdI GACnn_n'nnGTC
Cuts at: 2491 5017 6997 9575 2491
Size: 2526 1980 2578 3762
Fragments arranged by size:
3762 2578 2526 1980
AleI CACnn'nnGTG
Cuts at: 1166 3818 4142 4193 7287 7475 1166
Size: 2652 324 51 3094 188 4537
Fragments arranged by size:
4537 3094 2652 324 188 51
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5565 1184
Size: 4381 6465
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2897 2929 6341 6373 6653 6685 6941 6973 7229
Size: 32 3412 32 280 32 256 32 256
Cuts at: 7229 7261 2897
Size: 32 6482
Fragments arranged by size:
6482 3412 280 256 256 32 32 32
32 32
AlwNI CAG_nnn'CTG
Cuts at: 2118 2204 2392 3760 4034 4273 6664 8053 8068
Size: 86 188 1368 274 239 2391 1389 15
Cuts at: 8068 8589 10161 2118
Size: 521 1572 2803
Fragments arranged by size:
2803 2391 1572 1389 1368 521 274 239
188 86 15
ApaI G_GGCC'C
Cuts at: 2217 3303 4564 5062 5561 6331 7824 2217
Size: 1086 1261 498 499 770 1493 5239
Fragments arranged by size:
5239 1493 1261 1086 770 499 498
ApaLI G'TGCA_C
Cuts at: 3200 4154 4196 5420 6866 7181 8903 10059 3200
Size: 954 42 1224 1446 315 1722 1156 3987
Fragments arranged by size:
3987 1722 1446 1224 1156 954 315 42
ApoI r'AATT_y
Cuts at: 1493 2234 3794 4181 5132 8155 8381 8533 10642
Size: 741 1560 387 951 3023 226 152 2109
Cuts at: 10642 1493
Size: 1697
Fragments arranged by size:
3023 2109 1697 1560 951 741 387 226
152
AseI AT'TA_AT
Cuts at: 160 1032 9400 160
Size: 872 8368 1606
Fragments arranged by size:
8368 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10846
AvaI C'yCGr_G
Cuts at: 1870 1943 2082 2210 2271 2294 2530 3842 5685
Size: 73 139 128 61 23 236 1312 1843
Cuts at: 5685 6228 6762 6905 7604 8171 1870
Size: 543 534 143 699 567 4545
Fragments arranged by size:
4545 1843 1312 699 567 543 534 236
143 139 128 73 61 23
BamHI G'GATC_C
Cuts at: 2298 3978 8176 8543 2298
Size: 1680 4198 367 4601
Fragments arranged by size:
4601 4198 1680 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2564 2713 2942 3444 3999
Size: 325 217 627 777 149 229 502 555
Cuts at: 3999 5627 5984 7277 8083 8167 9622 618
Size: 1628 357 1293 806 84 1455 1842
Fragments arranged by size:
1842 1628 1455 1293 806 777 627 555
502 357 325 229 217 149 84
BanII G_rGCy'C
Cuts at: 1391 2163 2211 2217 2295 2537 2832 2938 3303
Size: 772 48 6 78 242 295 106 365
Cuts at: 3303 3701 4137 4545 4564 4925 5062 5249 5340
Size: 398 436 408 19 361 137 187 91
Cuts at: 5340 5561 5998 6035 6331 6779 7101 7506 7814
Size: 221 437 37 296 448 322 405 308
Cuts at: 7814 7824 8165 1391
Size: 10 341 4072
Fragments arranged by size:
4072 772 448 437 436 408 405 398
365 361 341 322 308 296 295 242
221 187 137 106 91 78 48 37
19 10 6
BbeI G_GCGC'C
Cuts at: 3448 4003 7281 3448
Size: 555 3278 7013
Fragments arranged by size:
7013 3278 555
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2550 5035 7745 7887 928
Size: 971 651 2485 2710 142 3887
Fragments arranged by size:
3887 2710 2485 971 651 142
BbvCI CC'TCA_GC
Cuts at: 5553 6612 5553
Size: 1059 9787
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3166 3200 3608 3642 4892 4926 9037
Size: 34 1229 34 408 34 1250 34 4111
Cuts at: 9037 9071 1903
Size: 34 3678
Fragments arranged by size:
4111 3678 1250 1229 408 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5518 8738 9948 5518
Size: 3220 1210 6416
Fragments arranged by size:
6416 3220 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 10846
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3194 3696 4533
Size: 107 122 71 631 605 1522 502 837
Cuts at: 4533 4592 4994 6884 7582 9457 136
Size: 59 402 1890 698 1875 1525
Fragments arranged by size:
1890 1875 1525 1522 837 698 631 605
502 402 122 107 71 59
BglII A'GATC_T
Cuts at: 2877 7347 10841 2877
Size: 4470 3494 2882
Fragments arranged by size:
4470 3494 2882
BlpI GC'TnA_GC
Cuts at: 2205 3396 4247 7631 8263 2205
Size: 1191 851 3384 632 4788
Fragments arranged by size:
4788 3384 1191 851 632
Bme1580I G_kGCm'C
Cuts at: 1790 2217 3204 3303 3947 4158 4200 4564 5062
Size: 427 987 99 644 211 42 364 498
Cuts at: 5062 5424 5561 5737 6286 6295 6331 6870 7185
Size: 362 137 176 549 9 36 539 315
Cuts at: 7185 7824 8907 10063 1790
Size: 639 1083 1156 2573
Fragments arranged by size:
2573 1156 1083 987 644 639 549 539
498 427 364 362 315 211 176 137
99 42 36 9
BmgBI CAC'GTC
Cuts at: 1318 4201 1318
Size: 2883 7963
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2371 4022 6850
Size: 416 53 83 399 24 954 1651 2828
Cuts at: 6850 8577 9535 442
Size: 1727 958 1753
Fragments arranged by size:
2828 1753 1727 1651 958 954 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10846
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2092 2502 3623 3974 4226 4481 4551 4772
Size: 853 410 1121 351 252 255 70 221
Cuts at: 4772 4955 5072 5187 5242 6212 7382 7865 8033
Size: 183 117 115 55 970 1170 483 168
Cuts at: 8033 9506 1239
Size: 1473 2579
Fragments arranged by size:
2579 1473 1170 1121 970 853 483 410
351 255 252 221 183 168 117 115
70 55
Bpu10I CC'TnA_GC
Cuts at: 3543 5553 6612 3543
Size: 2010 1059 7777
Fragments arranged by size:
7777 2010 1059
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2792 3476 4217 7490 8971 9836 10134 2792
Size: 684 741 3273 1481 865 298 3504
Fragments arranged by size:
3504 3273 1481 865 741 684 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 9509 882
Size: 617 8010 2219
Fragments arranged by size:
8010 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3661 4339 5167 7473 8637 493
Size: 711 2457 678 828 2306 1164 2702
Fragments arranged by size:
2702 2457 2306 1164 828 711 678
BsaBI GATnn'nnATC
Cuts at: 6737 10840 6737
Size: 4103 6743
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3330 3445 4000 5851
Size: 53 83 186 936 1797 115 555 1851
Cuts at: 5851 7278 7395 7703 8653 9035 9670 275
Size: 1427 117 308 950 382 635 1451
Fragments arranged by size:
1851 1797 1451 1427 950 936 635 555
382 308 186 117 115 83 53
BsaWI w'CCGG_w
Cuts at: 3817 4452 6781 9279 9677 9951 10098 10509 3817
Size: 635 2329 2498 398 274 147 411 4154
Fragments arranged by size:
4154 2498 2329 635 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2897 2927 6341 6371 7229
Size: 30 165 30 1494 30 3414 30 858
Cuts at: 7229 7259 7298 7328 1178
Size: 30 39 30 4696
Fragments arranged by size:
4696 3414 1494 858 165 39 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3869 4223 4748 6043 6377 6629 6986 7025
Size: 2683 354 525 1295 334 252 357 39
Cuts at: 7025 7379 7703 1186
Size: 354 324 4329
Fragments arranged by size:
4329 2683 1295 525 357 354 354 334
324 252 39
BseYI C'CCAG_C
Cuts at: 2239 3227 3486 3749 4052 4098 4544 5333 5817
Size: 988 259 263 303 46 446 789 484
Cuts at: 5817 5841 5845 6832 7045 7233 8061 8073 10049
Size: 24 4 987 213 188 828 12 1976
Cuts at: 10049 10545 2239
Size: 496 2540
Fragments arranged by size:
2540 1976 988 987 828 789 496 484
446 303 263 259 213 188 46 24
12 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2418 3139 3746 4598 4820 5170 6224
Size: 573 682 721 607 852 222 350 1054
Cuts at: 6224 6662 7546 1163
Size: 438 884 4463
Fragments arranged by size:
4463 1054 884 852 721 682 607 573
438 350 222
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3144 3198 3941 4143 6876 8296
Size: 275 186 1143 54 743 202 2733 1420
Cuts at: 8296 9057 10085 1540
Size: 761 1028 2301
Fragments arranged by size:
2733 2301 1420 1143 1028 761 743 275
202 186 54
BsiHKAI G_wGCw'C
Cuts at: 2772 3204 4068 4137 4158 4200 4980 5163 5249
Size: 432 864 69 21 42 780 183 86
Cuts at: 5249 5424 5998 6576 6870 7185 7506 8907 8992
Size: 175 574 578 294 315 321 1401 85
Cuts at: 8992 10063 2772
Size: 1071 3555
Fragments arranged by size:
3555 1401 1071 864 780 578 574 432
321 315 294 183 175 86 85 69
42 21
BsiWI C'GTAC_G
Cuts at: 4771 4771
Size: 10846
BsmI GAATG_Cn'
Cuts at: 2282 5387 8357 8450 2282
Size: 3105 2970 93 4678
Fragments arranged by size:
4678 3105 2970 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2478 7685 2478
Size: 5207 5639
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2163 2211 2217 2295 2537 2772 2832
Size: 399 373 48 6 78 242 235 60
Cuts at: 2832 2938 3204 3303 3701 3947 4068 4137 4158
Size: 106 266 99 398 246 121 69 21
Cuts at: 4158 4200 4545 4564 4925 4980 5062 5163 5249
Size: 42 345 19 361 55 82 101 86
Cuts at: 5249 5340 5424 5561 5737 5998 6035 6286 6295
Size: 91 84 137 176 261 37 251 9
Cuts at: 6295 6331 6576 6779 6870 7101 7185 7506 7814
Size: 36 245 203 91 231 84 321 308
Cuts at: 7814 7824 8165 8907 8992 10063 1391
Size: 10 341 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 398 373 361 345
341 321 308 266 261 251 246 245
242 235 231 203 176 137 121 106
101 99 91 91 86 85 84 84
82 78 69 60 55 48 42 37
36 21 19 10 9 6
BspEI T'CCGG_A
Cuts at: 10509 10509
Size: 10846
BspHI T'CATG_A
Cuts at: 8735 10465 8735
Size: 1730 9116
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2065 3660 3909 4848 5062 5508 6471 6508
Size: 1221 1595 249 939 214 446 963 37
Cuts at: 6508 6585 6643 6684 6930 8201 844
Size: 77 58 41 246 1271 3489
Fragments arranged by size:
3489 1595 1271 1221 963 939 446 249
246 214 77 58 41 37
BsrBI CCG'CTC
Cuts at: 1394 1823 2629 2973 8733 1394
Size: 429 806 344 5760 3507
Fragments arranged by size:
5760 3507 806 429 344
BsrDI GCAATG_nn'
Cuts at: 65 1248 2606 2665 4431 5619 5655 9341 9515
Size: 1183 1358 59 1766 1188 36 3686 174
Cuts at: 9515 65
Size: 1396
Fragments arranged by size:
3686 1766 1396 1358 1188 1183 174 59
36
BsrFI r'CCGG_y
Cuts at: 1700 1810 2190 2652 2682 3267 3582 3763 3817
Size: 110 380 462 30 585 315 181 54
Cuts at: 3817 4010 5800 7076 7356 9490 9677 1700
Size: 193 1790 1276 280 2134 187 2869
Fragments arranged by size:
2869 2134 1790 1276 585 462 380 315
280 193 187 181 110 54 30
BsrGI T'GTAC_A
Cuts at: 96 6267 6750 7314 96
Size: 6171 483 564 3628
Fragments arranged by size:
6171 3628 564 483
BssHII G'CGCG_C
Cuts at: 1925 7585 1925
Size: 5660 5186
BssSI C'ACGA_G
Cuts at: 4352 4730 5156 5481 6131 6881 8906 9918 4352
Size: 378 426 325 650 750 2025 1012 5280
Fragments arranged by size:
5280 2025 1012 750 650 426 378 325
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2118 4273 4843 5148 5428 5732 8025 8053
Size: 1324 2155 570 305 280 304 2293 28
Cuts at: 8053 794
Size: 3587
Fragments arranged by size:
3587 2293 2155 1324 570 305 304 280
28
BstBI TT'CG_AA
Cuts at: 5295 9650 5295
Size: 4355 6491
BstEII G'GTnAC_C
Cuts at: 1184 5281 6520 7289 7516 1184
Size: 4097 1239 769 227 4514
Fragments arranged by size:
4514 4097 1239 769 227
BstYI r'GATC_y
Cuts at: 1972 2298 2784 2841 2877 3978 7347 7872 8176
Size: 326 486 57 36 1101 3369 525 304
Cuts at: 8176 8543 8928 8945 10397 10483 10495 10841 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
3369 1977 1452 1101 525 486 385 367
346 326 304 86 57 36 17 12
Bsu36I CC'TnA_GG
Cuts at: 2219 2502 2585 4004 5373 6395 8102 2219
Size: 283 83 1419 1369 1022 1707 4963
Fragments arranged by size:
4963 1707 1419 1369 1022 283 83
BtgI C'CryG_G
Cuts at: 513 1065 2004 2286 2337 2733 3963 4408 4480
Size: 552 939 282 51 396 1230 445 72
Cuts at: 4480 4597 4671 5534 5649 6927 7089 7176 513
Size: 117 74 863 115 1278 162 87 4183
Fragments arranged by size:
4183 1278 1230 939 863 552 445 396
282 162 117 115 87 74 72 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 10107
Fragments arranged by size:
10107 683 56
BtsI GCAGTG_nn'
Cuts at: 2098 2566 3796 3945 3983 4292 4486 4754 4771
Size: 468 1230 149 38 309 194 268 17
Cuts at: 4771 4908 5504 6255 6286 8362 9156 9176 2098
Size: 137 596 751 31 2076 794 20 3768
Fragments arranged by size:
3768 2076 1230 794 751 596 468 309
268 194 149 137 38 31 20 17
ClaI AT'CG_AT
Cuts at: 1554 7328 1554
Size: 5774 5072
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 6156 6191 529
Size: 35 5592 35 5184
Fragments arranged by size:
5592 5184 35 35
DraI TTT'AAA
Cuts at: 8150 8150
Size: 10846
DraIII CAC_nnn'GTG
Cuts at: 4339 5167 4339
Size: 828 10018
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 9853 809
Size: 507 8537 1802
Fragments arranged by size:
8537 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2055 2192 3141
Size: 54 1425 50 275 18 225 137 949
Cuts at: 3141 3195 3207 3269 3938 4140 4302 4387 4713
Size: 54 12 62 669 202 162 85 326
Cuts at: 4713 4849 4971 5076 5437 5487 5646 5652 6315
Size: 136 122 105 361 50 159 6 663
Cuts at: 6315 6490 6813 6873 7173 7716 8293 9182 10811
Size: 175 323 60 300 543 577 889 1629
Cuts at: 10811 8
Size: 43
Fragments arranged by size:
1629 1425 949 889 669 663 577 543
361 326 323 300 275 225 202 175
162 159 137 136 122 105 85 62
60 54 54 50 50 43 18 12
6
EagI C'GGCC_G
Cuts at: 1537 1812 3141 3195 3938 4140 6873 8293 1537
Size: 275 1329 54 743 202 2733 1420 4090
Fragments arranged by size:
4090 2733 1420 1329 743 275 202 54
EarI CTCTTCn'nnn_
Cuts at: 1353 3425 4817 6062 6302 6564 7898 8776 1353
Size: 2072 1392 1245 240 262 1334 878 3423
Fragments arranged by size:
3423 2072 1392 1334 1245 878 262 240
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6079 6967 9433 9807 9953 387
Size: 1064 138 4490 888 2466 374 146 1280
Fragments arranged by size:
4490 2466 1280 1064 888 374 146 138
EcoICRI GAG'CTC
Cuts at: 4135 5247 5996 7504 4135
Size: 1112 749 1508 7477
Fragments arranged by size:
7477 1508 1112 749
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2092 2169 2502 3026 3623 3667 3974
Size: 393 460 77 333 524 597 44 307
Cuts at: 3974 4226 4481 4551 4772 4955 5029 5072 5187
Size: 252 255 70 221 183 74 43 115
Cuts at: 5187 5242 5400 6212 6275 6547 7382 7790 7865
Size: 55 158 812 63 272 835 408 75
Cuts at: 7865 8033 8909 9506 10293 10561 1239
Size: 168 876 597 787 268 1524
Fragments arranged by size:
1524 876 835 812 787 597 597 524
460 408 393 333 307 272 268 255
252 221 183 168 158 115 77 75
74 70 63 55 44 43
EcoNI CCTnn'n_nnAGG
Cuts at: 4208 6905 8036 4208
Size: 2697 1131 7018
Fragments arranged by size:
7018 2697 1131
EcoO109I rG'GnC_Cy
Cuts at: 2137 2214 2224 2446 3299 3300 3415 3745 4165
Size: 77 10 222 853 1 115 330 420
Cuts at: 4165 4371 4560 4561 5557 6327 6328 6661 6724
Size: 206 189 1 996 770 1 333 63
Cuts at: 6724 7771 7820 7838 7972 2137
Size: 1047 49 18 134 5011
Fragments arranged by size:
5011 1047 996 853 770 420 333 330
222 206 189 134 115 77 63 49
18 10 1 1 1
EcoRI G'AATT_C
Cuts at: 2234 5132 8155 8533 2234
Size: 2898 3023 378 4547
Fragments arranged by size:
4547 3023 2898 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4897 4897
Size: 10846
FseI GG_CCGG'CC
Cuts at: 3586 3586
Size: 10846
FspI TGC'GCA
Cuts at: 3707 5126 9352 3707
Size: 1419 4226 5201
Fragments arranged by size:
5201 4226 1419
HaeII r_GCGC'y
Cuts at: 1678 2023 2119 3228 3268 3448 4003 4085 4835
Size: 345 96 1109 40 180 555 82 750
Cuts at: 4835 5807 5818 6453 7281 8068 9993 1678
Size: 972 11 635 828 787 1925 2531
Fragments arranged by size:
2531 1925 1109 972 828 787 750 635
555 345 180 96 82 40 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2477 2509 2769 2801 2833
Size: 32 135 32 905 32 260 32 32
Cuts at: 2833 2865 2891 2923 3230 3236 3262 3268 5003
Size: 32 26 32 307 6 26 6 1735
Cuts at: 5003 5035 6573 6605 6983 7015 9487 9519 9561
Size: 32 1538 32 378 32 2472 32 42
Cuts at: 9561 9593 1373
Size: 32 2626
Fragments arranged by size:
2626 2472 1735 1538 905 378 307 260
135 42 32 32 32 32 32 32
32 32 32 32 32 32 26 26
6 6
HincII GTy'rAC
Cuts at: 669 1657 1938 8190 8436 669
Size: 988 281 6252 246 3079
Fragments arranged by size:
6252 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 7439 748
Size: 6691 4155
HpaI GTT'AAC
Cuts at: 8436 8436
Size: 10846
KasI G'GCGC_C
Cuts at: 3444 3999 7277 3444
Size: 555 3278 7013
Fragments arranged by size:
7013 3278 555
KpnI G_GTAC'C
Cuts at: 5631 5988 8171 5631
Size: 357 2183 8306
Fragments arranged by size:
8306 2183 357
MfeI C'AATT_G
Cuts at: 8445 8445
Size: 10846
MluI A'CGCG_T
Cuts at: 10722 10722
Size: 10846
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3009 5838 6984 7011 9960 10144 1305
Size: 1704 2829 1146 27 2949 184 2007
Fragments arranged by size:
2949 2829 2007 1704 1146 184 27
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3209 4304 4389 4715 4851
Size: 54 1425 343 1377 1095 85 326 136
Cuts at: 4851 5078 5439 5648 5654 6317 7718 10
Size: 227 361 209 6 663 1401 3138
Fragments arranged by size:
3138 1425 1401 1377 1095 663 361 343
326 227 209 136 85 54 6
MslI CAynn'nnrTG
Cuts at: 518 1166 3818 4142 4193 4959 7287 7475 7673
Size: 648 2652 324 51 766 2328 188 198
Cuts at: 7673 8804 9163 9322 518
Size: 1131 359 159 2042
Fragments arranged by size:
2652 2328 2042 1131 766 648 359 324
198 188 159 51
MspA1I CmG'CkG
Cuts at: 1606 2243 2339 2431 2600 3235 3786 3927 4482
Size: 637 96 92 169 635 551 141 555
Cuts at: 4482 4498 5569 5845 7178 8077 8262 8939 10087
Size: 16 1071 276 1333 899 185 677 1148
Cuts at: 10087 10332 1606
Size: 245 2120
Fragments arranged by size:
2120 1333 1148 1071 899 677 637 635
555 551 276 245 185 169 141 96
92 16
NaeI GCC'GGC
Cuts at: 2192 3269 3584 5802 7078 2192
Size: 1077 315 2218 1276 5960
Fragments arranged by size:
5960 2218 1276 1077 315
NarI GG'CG_CC
Cuts at: 3445 4000 7278 3445
Size: 555 3278 7013
Fragments arranged by size:
7013 3278 555
NcoI C'CATG_G
Cuts at: 513 1065 2004 5649 7089 513
Size: 552 939 3645 1440 4270
Fragments arranged by size:
4270 3645 1440 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 10846
NgoMIV G'CCGG_C
Cuts at: 2190 3267 3582 5800 7076 2190
Size: 1077 315 2218 1276 5960
Fragments arranged by size:
5960 2218 1276 1077 315
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10846
NotI GC'GGCC_GC
Cuts at: 6873 8293 6873
Size: 1420 9426
NspI r_CATG'y
Cuts at: 1132 1229 3181 4096 5293 7732 8204 9749 1132
Size: 97 1952 915 1197 2439 472 1545 2229
Fragments arranged by size:
2439 2229 1952 1545 1197 915 472 97
PasI CC'CwG_GG
Cuts at: 1348 2131 3576 5701 1348
Size: 783 1445 2125 6493
Fragments arranged by size:
6493 2125 1445 783
PciI A'CATG_T
Cuts at: 9745 9745
Size: 10846
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4342 4816 6862 8080 1108
Size: 3234 474 2046 1218 3874
Fragments arranged by size:
3874 3234 2046 1218 474
PfoI T'CCnGG_A
Cuts at: 4203 4551 4896 6345 6657 7025 7143 7875 4203
Size: 348 345 1449 312 368 118 732 7174
Fragments arranged by size:
7174 1449 732 368 348 345 312 118
PmeI GTTT'AAAC
Cuts at: 8150 8150
Size: 10846
PmlI CAC'GTG
Cuts at: 4339 4339
Size: 10846
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2897 2929 6341 6373 7229 7261 8915 8947 10428
Size: 32 3412 32 856 32 1654 32 1481
Cuts at: 10428 10460 2897
Size: 32 3283
Fragments arranged by size:
3412 3283 1654 1481 856 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 2137 2224 3415 3745 4165 6661 6724 7771 7838
Size: 87 1191 330 420 2496 63 1047 67
Cuts at: 7838 2137
Size: 5145
Fragments arranged by size:
5145 2496 1191 1047 420 330 87 67
63
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 10846
PsiI TTA'TAA
Cuts at: 8416 8416
Size: 10846
PspOMI G'GGCC_C
Cuts at: 2213 3299 4560 5058 5557 6327 7820 2213
Size: 1086 1261 498 499 770 1493 5239
Fragments arranged by size:
5239 1493 1261 1086 770 499 498
PspXI vC'TCGA_Gb
Cuts at: 6905 6905
Size: 10846
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3641 3673 3641
Size: 32 10814
PstI C_TGCA'G
Cuts at: 830 2123 2268 3338 3791 3993 4749 5814 7197
Size: 1293 145 1070 453 202 756 1065 1383
Cuts at: 7197 8049 8198 830
Size: 852 149 3478
Fragments arranged by size:
3478 1383 1293 1070 1065 852 756 453
202 149 145
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10846
PvuII CAG'CTG
Cuts at: 2243 2600 3235 3786 3927 4498 5845 8077 2243
Size: 357 635 551 141 571 1347 2232 5012
Fragments arranged by size:
5012 2232 1347 635 571 551 357 141
SacI G_AGCT'C
Cuts at: 4137 5249 5998 7506 4137
Size: 1112 749 1508 7477
Fragments arranged by size:
7477 1508 1112 749
SacII CC_GC'GG
Cuts at: 2340 4483 7179 2340
Size: 2143 2696 6007
Fragments arranged by size:
6007 2696 2143
SalI G'TCGA_C
Cuts at: 1936 8188 1936
Size: 6252 4594
SanDI GG'GwC_CC
Cuts at: 2137 7838 2137
Size: 5701 5145
SapI GCTCTTCn'nnn_
Cuts at: 6062 6564 7898 6062
Size: 502 1334 9010
Fragments arranged by size:
9010 1334 502
SbfI CC_TGCA'GG
Cuts at: 8198 8198
Size: 10846
ScaI AGT'ACT
Cuts at: 9094 10806 9094
Size: 1712 9134
SexAI A'CCwGG_T
Cuts at: 2226 6797 2226
Size: 4571 6275
SfcI C'TryA_G
Cuts at: 826 1045 2119 2264 2743 3334 3643 3787 3989
Size: 219 1074 145 479 591 309 144 202
Cuts at: 3989 4745 5810 7193 7793 7815 8045 8194 9329
Size: 756 1065 1383 600 22 230 149 1135
Cuts at: 9329 10010 10201 826
Size: 681 191 1471
Fragments arranged by size:
1471 1383 1135 1074 1065 756 681 600
591 479 309 230 219 202 191 149
145 144 22
SfoI GGC'GCC
Cuts at: 3446 4001 7279 3446
Size: 555 3278 7013
Fragments arranged by size:
7013 3278 555
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10846
SgrAI Cr'CCGG_yG
Cuts at: 1700 3817 1700
Size: 2117 8729
SmaI CCC'GGG
Cuts at: 1872 2084 2212 2273 2296 5687 6230 6764 8173
Size: 212 128 61 23 3391 543 534 1409
Cuts at: 8173 1872
Size: 4545
Fragments arranged by size:
4545 3391 1409 543 534 212 128 61
23
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2807 3491 4232 6905 7505 8950
Size: 197 926 864 684 741 2673 600 1445
Cuts at: 8950 9851 10113 820
Size: 901 262 1553
Fragments arranged by size:
2673 1553 1445 926 901 864 741 684
600 262 197
SnaBI TAC'GTA
Cuts at: 493 8637 493
Size: 8144 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10846
SphI G_CATG'C
Cuts at: 1132 3181 4096 8204 1132
Size: 2049 915 4108 3774
Fragments arranged by size:
4108 3774 2049 915
SrfI GCCC'GGGC
Cuts at: 2212 2212
Size: 10846
SspI AAT'ATT
Cuts at: 5 52 8770 5
Size: 47 8718 2081
Fragments arranged by size:
8718 2081 47
StuI AGG'CCT
Cuts at: 1517 3809 5909 6137 7202 7457 1517
Size: 2292 2100 228 1065 255 4906
Fragments arranged by size:
4906 2292 2100 1065 255 228
StyI C'CwwG_G
Cuts at: 513 1065 2004 2304 3591 4914 5277 5649 6114
Size: 552 939 300 1287 1323 363 372 465
Cuts at: 6114 6138 7089 8285 513
Size: 24 951 1196 3074
Fragments arranged by size:
3074 1323 1287 1196 951 939 552 465
372 363 300 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2960 4090 4741 5770 6335 8101 8873
Size: 1255 1380 1130 651 1029 565 1766 772
Cuts at: 8873 8890 9043 9228 9673 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1766 1498 1380 1255 1130 1029 772 651
565 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2179 3729 5892
Size: 274 80 33 51 1443 202 1550 2163
Cuts at: 5892 6267 6750 7314 9092 10804 96
Size: 375 483 564 1778 1712 138
Fragments arranged by size:
2163 1778 1712 1550 1443 564 483 375
274 202 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7211 7243 10806 10838 7211
Size: 32 3563 32 7219
Fragments arranged by size:
7219 3563 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2363 3471 7394 1316
Size: 1047 1108 3923 4768
Fragments arranged by size:
4768 3923 1108 1047
XbaI T'CTAG_A
Cuts at: 8182 8182
Size: 10846
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2962 3598 6121 2962
Size: 636 2523 7687
Fragments arranged by size:
7687 2523 636
XhoI C'TCGA_G
Cuts at: 1943 6905 1943
Size: 4962 5884
XmaI C'CCGG_G
Cuts at: 1870 2082 2210 2271 2294 5685 6228 6762 8171
Size: 212 128 61 23 3391 543 534 1409
Cuts at: 8171 1870
Size: 4545
Fragments arranged by size:
4545 3391 1409 543 534 212 128 61
23
XmnI GAAnn'nnTTC
Cuts at: 2667 5976 6009 7034 8975 2667
Size: 3309 33 1025 1941 4538
Fragments arranged by size:
4538 3309 1941 1025 33
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5852 7396 8654 276
Size: 53 83 186 936 4318 1544 1258 2468
Fragments arranged by size:
4318 2468 1544 1258 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BplI BstXI BstZ17I EcoRV
FspAI NruI NsiI PacI RsrII SfiI SwaI