Restriction map for
pFN21AA1390
(Circular) (Six-base) MAPSORT of: pFN21AA1390.seq Check: 732 from: 1 to: 6112
pFN21AA1390 6112 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2813 2813
Size: 6112
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3922 278
Size: 53 83 186 936 2386 2468
Fragments arranged by size:
2468 2386 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2669 3171 3455 1937
Size: 732 502 284 4594
Fragments arranged by size:
4594 732 502 284
Acc65I G'GTAC_C
Cuts at: 3433 3433
Size: 6112
AclI AA'CG_TT
Cuts at: 1563 4239 4612 1563
Size: 2676 373 3063
Fragments arranged by size:
3063 2676 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2238 2283 2432 2546 3245 4175 5559 5827
Size: 606 45 149 114 699 930 1384 268
Cuts at: 5827 1632
Size: 1917
Fragments arranged by size:
1917 1384 930 699 606 268 149 114
45
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5915
AflIII A'CryG_T
Cuts at: 1203 5011 5988 1203
Size: 3808 977 1327
Fragments arranged by size:
3808 1327 977
AgeI A'CCGG_T
Cuts at: 4943 4943
Size: 6112
AhdI GACnn_n'nnGTC
Cuts at: 4841 4841
Size: 6112
AleI CACnn'nnGTG
Cuts at: 1166 2677 2817 1166
Size: 1511 140 4461
Fragments arranged by size:
4461 1511 140
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6112
AlwNI CAG_nnn'CTG
Cuts at: 2036 2863 2918 3855 5427 2036
Size: 827 55 937 1572 2721
Fragments arranged by size:
2721 1572 937 827 55
ApaLI G'TGCA_C
Cuts at: 4169 5325 4169
Size: 1156 4956
ApoI r'AATT_y
Cuts at: 1493 3421 3647 3799 5908 1493
Size: 1928 226 152 2109 1697
Fragments arranged by size:
2109 1928 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4666 160
Size: 872 3634 1606
Fragments arranged by size:
3634 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6112
AvaI C'yCGr_G
Cuts at: 1870 1943 2675 3437 1870
Size: 73 732 762 4545
Fragments arranged by size:
4545 762 732 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2696 2729 2696
Size: 33 6079
BamHI G'GATC_C
Cuts at: 2026 2358 3442 3809 2026
Size: 332 1084 367 4329
Fragments arranged by size:
4329 1084 367 332
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3433 4888 618
Size: 325 217 627 1646 1455 1842
Fragments arranged by size:
1842 1646 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2502 3431 1391
Size: 1111 929 4072
Fragments arranged by size:
4072 1111 929
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5141
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4303 4337 1903
Size: 34 2366 34 3678
Fragments arranged by size:
3678 2366 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4004 5214 4004
Size: 1210 4902
BclI T'GATC_A
Cuts at: 1548 2484 1548
Size: 936 5176
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4723 136
Size: 107 122 71 631 605 3051 1525
Fragments arranged by size:
3051 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6107 6107
Size: 6112
BlpI GC'TnA_GC
Cuts at: 2227 3529 2227
Size: 1302 4810
Bme1580I G_kGCm'C
Cuts at: 1790 4173 5329 1790
Size: 2383 1156 2573
Fragments arranged by size:
2573 2383 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6112
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3843 4801 442
Size: 416 53 83 399 24 2426 958 1753
Fragments arranged by size:
2426 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2226 1056
Size: 1170 4942
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2800 2885 3147 4772 1239
Size: 1561 85 262 1625 2579
Fragments arranged by size:
2579 1625 1561 262 85
Bpu10I CC'TnA_GC
Cuts at: 2331 2922 2331
Size: 591 5521
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2222 4237 5102 5400 2222
Size: 2015 865 298 2934
Fragments arranged by size:
2934 2015 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2861 4775 882
Size: 617 1362 1914 2219
Fragments arranged by size:
2219 1914 1362 617
BsaAI yAC'GTr
Cuts at: 493 1204 3903 493
Size: 711 2699 2702
Fragments arranged by size:
2702 2699 711
BsaBI GATnn'nnATC
Cuts at: 6106 6106
Size: 6112
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2652 3355 3919 4301
Size: 53 83 186 936 1119 703 564 382
Cuts at: 4301 4936 275
Size: 635 1451
Fragments arranged by size:
1451 1119 936 703 635 564 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4545 4943 5217 5364 5775 4545
Size: 398 274 147 411 4882
Fragments arranged by size:
4882 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5887
Fragments arranged by size:
5887 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3044 3284 3354 1186
Size: 1858 240 70 3944
Fragments arranged by size:
3944 1858 240 70
BseYI C'CCAG_C
Cuts at: 2880 3226 3252 3358 5315 5811 2880
Size: 346 26 106 1957 496 3181
Fragments arranged by size:
3181 1957 496 346 106 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2701 2778 3038 1163
Size: 573 965 77 260 4237
Fragments arranged by size:
4237 965 573 260 77
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3562 4323 5351 1540
Size: 275 186 1561 761 1028 2301
Fragments arranged by size:
2301 1561 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2106 2402 2502 4173 4258 5329 2106
Size: 296 100 1671 85 1071 2889
Fragments arranged by size:
2889 1671 1071 296 100 85
BsmI GAATG_Cn'
Cuts at: 3402 3623 3716 3402
Size: 221 93 5798
Fragments arranged by size:
5798 221 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2659 2659
Size: 6112
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2106 2402 2502 3431 4173 4258 5329
Size: 399 316 296 100 929 742 85 1071
Cuts at: 5329 1391
Size: 2174
Fragments arranged by size:
2174 1071 929 742 399 316 296 100
85
BspEI T'CCGG_A
Cuts at: 5775 5775
Size: 6112
BspHI T'CATG_A
Cuts at: 2430 4001 5731 2430
Size: 1571 1730 2811
Fragments arranged by size:
2811 1730 1571
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2703 2813 3157 3467 844
Size: 1859 110 344 310 3489
Fragments arranged by size:
3489 1859 344 310 110
BsrBI CCG'CTC
Cuts at: 1394 1823 3999 1394
Size: 429 2176 3507
Fragments arranged by size:
3507 2176 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2601 4607 4781 65
Size: 1183 1353 2006 174 1396
Fragments arranged by size:
2006 1396 1353 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4756 4943 1700
Size: 110 2946 187 2869
Fragments arranged by size:
2946 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6112
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6112
BssSI C'ACGA_G
Cuts at: 2831 3216 3389 4172 5184 2831
Size: 385 173 783 1012 3759
Fragments arranged by size:
3759 1012 783 385 173
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2129 3301 794
Size: 1335 1172 3605
Fragments arranged by size:
3605 1335 1172
BstBI TT'CG_AA
Cuts at: 4916 4916
Size: 6112
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6112
BstXI CCAn_nnnn'nTGG
Cuts at: 2626 2626
Size: 6112
BstYI r'GATC_y
Cuts at: 1972 2026 2358 3442 3809 4194 4211 5663 5749
Size: 54 332 1084 367 385 17 1452 86
Cuts at: 5749 5761 6107 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1084 385 367 346 332 86
54 17 12
BstZ17I GTA'TAC
Cuts at: 2670 2670
Size: 6112
Bsu36I CC'TnA_GG
Cuts at: 2022 2109 2745 2022
Size: 87 636 5389
Fragments arranged by size:
5389 636 87
BtgI C'CryG_G
Cuts at: 513 1065 2004 2716 2874 2893 513
Size: 552 939 712 158 19 3732
Fragments arranged by size:
3732 939 712 552 158 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5373
Fragments arranged by size:
5373 683 56
BtsI GCAGTG_nn'
Cuts at: 2255 2689 3326 3628 4422 4442 2255
Size: 434 637 302 794 20 3925
Fragments arranged by size:
3925 794 637 434 302 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6112
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6077
DraI TTT'AAA
Cuts at: 3416 3416
Size: 6112
DraIII CAC_nnn'GTG
Cuts at: 3061 3061
Size: 6112
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5119 809
Size: 507 3803 1802
Fragments arranged by size:
3803 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2616 3559 4448
Size: 54 1425 50 275 18 786 943 889
Cuts at: 4448 6077 8
Size: 1629 43
Fragments arranged by size:
1629 1425 943 889 786 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3559 1537
Size: 275 1747 4090
Fragments arranged by size:
4090 1747 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4042 1353
Size: 2689 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4699 5073 5219 387
Size: 1064 138 3110 374 146 1280
Fragments arranged by size:
3110 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2500 2500
Size: 6112
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2238 2283 2432 2546 2800 2885 3147
Size: 393 606 45 149 114 254 85 262
Cuts at: 3147 3245 4175 4772 5559 5827 1239
Size: 98 930 597 787 268 1524
Fragments arranged by size:
1524 930 787 606 597 393 268 262
254 149 114 98 85 45
EcoNI CCTnn'n_nnAGG
Cuts at: 2367 3116 2367
Size: 749 5363
EcoO109I rG'GnC_Cy
Cuts at: 2319 3122 3319 2319
Size: 803 197 5112
Fragments arranged by size:
5112 803 197
EcoRI G'AATT_C
Cuts at: 3421 3799 3421
Size: 378 5734
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3007 3007
Size: 6112
FspI TGC'GCA
Cuts at: 4618 4618
Size: 6112
HaeII r_GCGC'y
Cuts at: 1678 5259 1678
Size: 3581 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4753 4785 4827 4859 1373
Size: 32 135 32 3181 32 42 32 2626
Fragments arranged by size:
3181 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3456 3702 669
Size: 988 281 1518 246 3079
Fragments arranged by size:
3079 1518 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2169 748
Size: 1421 4691
HpaI GTT'AAC
Cuts at: 3702 3702
Size: 6112
KpnI G_GTAC'C
Cuts at: 3437 3437
Size: 6112
MfeI C'AATT_G
Cuts at: 3711 3711
Size: 6112
MluI A'CGCG_T
Cuts at: 5988 5988
Size: 6112
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5226 5410 1305
Size: 3921 184 2007
Fragments arranged by size:
3921 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2618 10
Size: 54 1425 343 786 3504
Fragments arranged by size:
3504 1425 786 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2677 2817 4070 4429 4588 518
Size: 648 1511 140 1253 359 159 2042
Fragments arranged by size:
2042 1511 1253 648 359 159 140
MspA1I CmG'CkG
Cuts at: 1606 2785 2876 2884 2918 3075 3163 3226 3528
Size: 1179 91 8 34 157 88 63 302
Cuts at: 3528 4205 5353 5598 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1179 1148 677 302 245 157 91
88 63 34 8
NcoI C'CATG_G
Cuts at: 513 1065 2004 2716 513
Size: 552 939 712 3909
Fragments arranged by size:
3909 939 712 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6112
NheI G'CTAG_C
Cuts at: 1052 2222 1052
Size: 1170 4942
NotI GC'GGCC_GC
Cuts at: 3559 3559
Size: 6112
NspI r_CATG'y
Cuts at: 1132 1229 2286 3470 5015 1132
Size: 97 1057 1184 1545 2229
Fragments arranged by size:
2229 1545 1184 1057 97
PasI CC'CwG_GG
Cuts at: 1348 3112 1348
Size: 1764 4348
PciI A'CATG_T
Cuts at: 5011 5011
Size: 6112
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2036 2249 2788 1108
Size: 928 213 539 4432
Fragments arranged by size:
4432 928 539 213
PfoI T'CCnGG_A
Cuts at: 2862 2862
Size: 6112
PmeI GTTT'AAAC
Cuts at: 3416 3416
Size: 6112
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4181 4213 5694 5726 4181
Size: 32 1481 32 4567
Fragments arranged by size:
4567 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2319 3319 2319
Size: 1000 5112
PshAI GACnn'nnGTC
Cuts at: 1613 3317 1613
Size: 1704 4408
PsiI TTA'TAA
Cuts at: 3682 3682
Size: 6112
PstI C_TGCA'G
Cuts at: 830 2056 3168 3204 3333 3464 830
Size: 1226 1112 36 129 131 3478
Fragments arranged by size:
3478 1226 1112 131 129 36
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6112
PvuII CAG'CTG
Cuts at: 2785 2884 2918 3075 3163 2785
Size: 99 34 157 88 5734
Fragments arranged by size:
5734 157 99 88 34
SacI G_AGCT'C
Cuts at: 2502 2502
Size: 6112
SacII CC_GC'GG
Cuts at: 2877 2877
Size: 6112
SalI G'TCGA_C
Cuts at: 1936 3454 1936
Size: 1518 4594
SbfI CC_TGCA'GG
Cuts at: 3464 3464
Size: 6112
ScaI AGT'ACT
Cuts at: 4360 6072 4360
Size: 1712 4400
SfcI C'TryA_G
Cuts at: 826 1045 2052 3023 3164 3200 3329 3460 4595
Size: 219 1007 971 141 36 129 131 1135
Cuts at: 4595 5276 5467 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1007 971 681 219 191 141
131 129 36
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6112
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6112
SmaI CCC'GGG
Cuts at: 1872 3439 1872
Size: 1567 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2201 4216 5117 5379 820
Size: 197 926 258 2015 901 262 1553
Fragments arranged by size:
2015 1553 926 901 262 258 197
SnaBI TAC'GTA
Cuts at: 493 3903 493
Size: 3410 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6112
SphI G_CATG'C
Cuts at: 1132 2286 3470 1132
Size: 1154 1184 3774
Fragments arranged by size:
3774 1184 1154
SspI AAT'ATT
Cuts at: 5 52 4036 5
Size: 47 3984 2081
Fragments arranged by size:
3984 2081 47
StuI AGG'CCT
Cuts at: 1517 2844 1517
Size: 1327 4785
StyI C'CwwG_G
Cuts at: 513 1065 2004 2248 2363 2716 3551 513
Size: 552 939 244 115 353 835 3074
Fragments arranged by size:
3074 939 835 552 353 244 115
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2398 2408 4139 4156 4309 4494 4939
Size: 1255 818 10 1731 17 153 185 445
Cuts at: 4939 325
Size: 1498
Fragments arranged by size:
1731 1498 1255 818 445 185 153 17
10
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4358 6070 96
Size: 274 80 33 51 1443 2381 1712 138
Fragments arranged by size:
2381 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6072 6104 6072
Size: 32 6080
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6112
XbaI T'CTAG_A
Cuts at: 3448 3448
Size: 6112
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6112
XmaI C'CCGG_G
Cuts at: 1870 3437 1870
Size: 1567 4545
XmnI GAAnn'nnTTC
Cuts at: 4241 4241
Size: 6112
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3920 276
Size: 53 83 186 936 2386 2468
Fragments arranged by size:
2468 2386 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SacII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI
BplI BsiWI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI
RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI