Restriction map for
pFN21AA1386
(Circular) (Six-base) MAPSORT of: pFN21AA1386.seq Check: 9992 from: 1 to: 7972
pFN21AA1386 7972 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5782 278
Size: 53 83 186 936 4246 2468
Fragments arranged by size:
4246 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2707 4351 5315 1937
Size: 770 1644 964 4594
Fragments arranged by size:
4594 1644 964 770
Acc65I G'GTAC_C
Cuts at: 5293 5293
Size: 7972
AclI AA'CG_TT
Cuts at: 1563 6099 6472 1563
Size: 4536 373 3063
Fragments arranged by size:
4536 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2310 2652 2732 2790 3933 4098 4517 6035
Size: 678 342 80 58 1143 165 419 1518
Cuts at: 6035 7419 7687 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1518 1384 1143 678 419 342 268
165 80 58
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7775
AflIII A'CryG_T
Cuts at: 1203 3721 6871 7848 1203
Size: 2518 3150 977 1327
Fragments arranged by size:
3150 2518 1327 977
AgeI A'CCGG_T
Cuts at: 6803 6803
Size: 7972
AhdI GACnn_n'nnGTC
Cuts at: 6701 6701
Size: 7972
AleI CACnn'nnGTG
Cuts at: 1166 4442 1166
Size: 3276 4696
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2475 3166 3623 1184
Size: 1291 691 457 5533
Fragments arranged by size:
5533 1291 691 457
AlwNI CAG_nnn'CTG
Cuts at: 4373 4540 5715 7287 4373
Size: 167 1175 1572 5058
Fragments arranged by size:
5058 1572 1175 167
ApaLI G'TGCA_C
Cuts at: 2234 6029 7185 2234
Size: 3795 1156 3021
Fragments arranged by size:
3795 3021 1156
ApoI r'AATT_y
Cuts at: 1493 2024 3105 4038 4172 4298 5281 5507 5659
Size: 531 1081 933 134 126 983 226 152
Cuts at: 5659 7768 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1081 983 933 531 226 152
134 126
AseI AT'TA_AT
Cuts at: 160 1032 6526 160
Size: 872 5494 1606
Fragments arranged by size:
5494 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7972
AvaI C'yCGr_G
Cuts at: 1870 1943 2624 4191 4722 5297 1870
Size: 73 681 1567 531 575 4545
Fragments arranged by size:
4545 1567 681 575 531 73
BamHI G'GATC_C
Cuts at: 2620 5302 5669 2620
Size: 2682 367 4923
Fragments arranged by size:
4923 2682 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5293 6748 618
Size: 325 217 627 3506 1455 1842
Fragments arranged by size:
3506 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2133 2299 2788 3364 3901 4820 5291 1391
Size: 742 166 489 576 537 919 471 4072
Fragments arranged by size:
4072 919 742 576 537 489 471 166
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3042 4086 4563 928
Size: 971 1143 1044 477 4337
Fragments arranged by size:
4337 1143 1044 971 477
BbvCI CC'TCA_GC
Cuts at: 2088 2088
Size: 7972
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6163 6197 1903
Size: 34 4226 34 3678
Fragments arranged by size:
4226 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5864 7074 5864
Size: 1210 6762
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7972
BfrBI ATG'CAT
Cuts at: 2254 2254
Size: 7972
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6583 136
Size: 107 122 71 631 605 4911 1525
Fragments arranged by size:
4911 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7967 7967
Size: 7972
BlpI GC'TnA_GC
Cuts at: 5389 5389
Size: 7972
Bme1580I G_kGCm'C
Cuts at: 1790 2238 6033 7189 1790
Size: 448 3795 1156 2573
Fragments arranged by size:
3795 2573 1156 448
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7972
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2803 5703 6661
Size: 416 53 83 399 24 1386 2900 958
Cuts at: 6661 442
Size: 1753
Fragments arranged by size:
2900 1753 1386 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7972
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3915 4655 3915
Size: 740 7232
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3261 3702 4350 4956 6632 1239
Size: 2022 441 648 606 1676 2579
Fragments arranged by size:
2579 2022 1676 648 606 441
Bpu10I CC'TnA_GC
Cuts at: 2088 4814 2088
Size: 2726 5246
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3968 5148 5224 6097 6962 7260 3968
Size: 1180 76 873 865 298 4680
Fragments arranged by size:
4680 1180 873 865 298 76
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6635 882
Size: 617 5136 2219
Fragments arranged by size:
5136 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3724 5763 493
Size: 711 2520 2039 2702
Fragments arranged by size:
2702 2520 2039 711
BsaBI GATnn'nnATC
Cuts at: 2214 4276 7966 2214
Size: 2062 3690 2220
Fragments arranged by size:
3690 2220 2062
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5779 6161 6796 275
Size: 53 83 186 936 4246 382 635 1451
Fragments arranged by size:
4246 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6405 6803 7077 7224 7635 6405
Size: 398 274 147 411 6742
Fragments arranged by size:
6742 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7747
Fragments arranged by size:
7747 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2523 2745 3743 4327 4350 5214 1186
Size: 1337 222 998 584 23 864 3944
Fragments arranged by size:
3944 1337 998 864 584 222 23
BseYI C'CCAG_C
Cuts at: 3399 5001 7175 7671 3399
Size: 1602 2174 496 3700
Fragments arranged by size:
3700 2174 1602 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3037 4457 5013 1163
Size: 573 1301 1420 556 4122
Fragments arranged by size:
4122 1420 1301 573 556
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5422 6183 7211 1540
Size: 275 186 3421 761 1028 2301
Fragments arranged by size:
3421 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2238 2788 3364 3901 3963 4820 6033 6118 7189
Size: 550 576 537 62 857 1213 85 1071
Cuts at: 7189 2238
Size: 3021
Fragments arranged by size:
3021 1213 1071 857 576 550 537 85
62
BsmI GAATG_Cn'
Cuts at: 3978 5483 5576 3978
Size: 1505 93 6374
Fragments arranged by size:
6374 1505 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2133 2238 2299 2788 3364 3901 3963
Size: 399 343 105 61 489 576 537 62
Cuts at: 3963 4820 5291 6033 6118 7189 1391
Size: 857 471 742 85 1071 2174
Fragments arranged by size:
2174 1071 857 742 576 537 489 471
399 343 105 85 62 61
BspEI T'CCGG_A
Cuts at: 7635 7635
Size: 7972
BspHI T'CATG_A
Cuts at: 2788 4770 4936 5861 7591 2788
Size: 1982 166 925 1730 3169
Fragments arranged by size:
3169 1982 1730 925 166
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5327 844
Size: 4483 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5859 1394
Size: 429 4036 3507
Fragments arranged by size:
4036 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3350 6467 6641 65
Size: 1183 2102 3117 174 1396
Fragments arranged by size:
3117 2102 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6616 6803 1700
Size: 110 4806 187 2869
Fragments arranged by size:
4806 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4153 96
Size: 4057 3915
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7972
BssSI C'ACGA_G
Cuts at: 6032 7044 6032
Size: 1012 6960
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7972
BstBI TT'CG_AA
Cuts at: 6776 6776
Size: 7972
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7972
BstYI r'GATC_y
Cuts at: 1972 2620 2698 3329 5302 5669 6054 6071 7523
Size: 648 78 631 1973 367 385 17 1452
Cuts at: 7523 7609 7621 7967 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1973 1452 648 631 385 367 346
86 78 17 12
Bsu36I CC'TnA_GG
Cuts at: 2467 2694 4657 2467
Size: 227 1963 5782
Fragments arranged by size:
5782 1963 227
BtgI C'CryG_G
Cuts at: 513 1065 2004 4443 513
Size: 552 939 2439 4042
Fragments arranged by size:
4042 2439 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7233
Fragments arranged by size:
7233 683 56
BtsI GCAGTG_nn'
Cuts at: 3617 4592 5488 6282 6302 3617
Size: 975 896 794 20 5287
Fragments arranged by size:
5287 975 896 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7972
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3922 3957 529
Size: 35 3358 35 4544
Fragments arranged by size:
4544 3358 35 35
DraI TTT'AAA
Cuts at: 3820 5276 3820
Size: 1456 6516
DraIII CAC_nnn'GTG
Cuts at: 2885 2885
Size: 7972
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4329 6979 809
Size: 507 3013 2650 1802
Fragments arranged by size:
3013 2650 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4882 5419 6308
Size: 54 1425 50 275 18 3052 537 889
Cuts at: 6308 7937 8
Size: 1629 43
Fragments arranged by size:
3052 1629 1425 889 537 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5419 1537
Size: 275 3607 4090
Fragments arranged by size:
4090 3607 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2335 2947 3398 3648 4157 4486 4536 5902
Size: 982 612 451 250 509 329 50 1366
Cuts at: 5902 1353
Size: 3423
Fragments arranged by size:
3423 1366 982 612 509 451 329 250
50
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6559 6933 7079 387
Size: 1064 138 4970 374 146 1280
Fragments arranged by size:
4970 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2786 3362 3899 4818 2786
Size: 576 537 919 5940
Fragments arranged by size:
5940 919 576 537
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2310 2652 2732 2790 3261 3702 3933
Size: 393 678 342 80 58 471 441 231
Cuts at: 3933 4098 4350 4517 4956 6035 6632 7419 7687
Size: 165 252 167 439 1079 597 787 268
Cuts at: 7687 1239
Size: 1524
Fragments arranged by size:
1524 1079 787 678 597 471 441 439
393 342 268 252 231 167 165 80
58
EcoNI CCTnn'n_nnAGG
Cuts at: 3276 3276
Size: 7972
EcoO109I rG'GnC_Cy
Cuts at: 2270 4888 2270
Size: 2618 5354
EcoRI G'AATT_C
Cuts at: 4172 5281 5659 4172
Size: 1109 378 6485
Fragments arranged by size:
6485 1109 378
EcoRV GAT'ATC
Cuts at: 2286 3175 2286
Size: 889 7083
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2120 2315 2738 2795 3649 2120
Size: 195 423 57 854 6443
Fragments arranged by size:
6443 854 423 195 57
FspI TGC'GCA
Cuts at: 6478 6478
Size: 7972
HaeII r_GCGC'y
Cuts at: 1678 7119 1678
Size: 5441 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3719 3751 4859 4891 6613
Size: 32 135 32 2147 32 1108 32 1722
Cuts at: 6613 6645 6687 6719 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2147 1722 1108 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5316 5562 669
Size: 988 281 3378 246 3079
Fragments arranged by size:
3378 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2772 2817 748
Size: 2024 45 5903
Fragments arranged by size:
5903 2024 45
HpaI GTT'AAC
Cuts at: 5562 5562
Size: 7972
KpnI G_GTAC'C
Cuts at: 5297 5297
Size: 7972
MfeI C'AATT_G
Cuts at: 3526 3887 5571 3526
Size: 361 1684 5927
Fragments arranged by size:
5927 1684 361
MluI A'CGCG_T
Cuts at: 7848 7848
Size: 7972
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2853 3181 7086 7270 1305
Size: 1548 328 3905 184 2007
Fragments arranged by size:
3905 2007 1548 328 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4884 10
Size: 54 1425 343 3052 3098
Fragments arranged by size:
3098 3052 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3011 4442 5930 6289 6448 518
Size: 648 1845 1431 1488 359 159 2042
Fragments arranged by size:
2042 1845 1488 1431 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4880 5388 6065 7213 7458 1606
Size: 3274 508 677 1148 245 2120
Fragments arranged by size:
3274 2120 1148 677 508 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6481
Fragments arranged by size:
6481 939 552
NdeI CA'TA_TG
Cuts at: 387 2677 3145 4000 387
Size: 2290 468 855 4359
Fragments arranged by size:
4359 2290 855 468
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7972
NotI GC'GGCC_GC
Cuts at: 5419 5419
Size: 7972
NsiI A_TGCA'T
Cuts at: 2256 2256
Size: 7972
NspI r_CATG'y
Cuts at: 1132 1229 2746 3610 4149 5330 6875 1132
Size: 97 1517 864 539 1181 1545 2229
Fragments arranged by size:
2229 1545 1517 1181 864 539 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7972
PciI A'CATG_T
Cuts at: 6871 6871
Size: 7972
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2885 3264 1108
Size: 1777 379 5816
Fragments arranged by size:
5816 1777 379
PmeI GTTT'AAAC
Cuts at: 5276 5276
Size: 7972
PmlI CAC'GTG
Cuts at: 3724 3724
Size: 7972
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4326 4358 6041 6073 7554 7586 4326
Size: 32 1683 32 1481 32 4712
Fragments arranged by size:
4712 1683 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2270 4888 2270
Size: 2618 5354
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7972
PsiI TTA'TAA
Cuts at: 5542 5542
Size: 7972
PstI C_TGCA'G
Cuts at: 830 2358 2740 4210 5324 830
Size: 1528 382 1470 1114 3478
Fragments arranged by size:
3478 1528 1470 1114 382
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7972
PvuII CAG'CTG
Cuts at: 4880 4880
Size: 7972
SacI G_AGCT'C
Cuts at: 2788 3364 3901 4820 2788
Size: 576 537 919 5940
Fragments arranged by size:
5940 919 576 537
SalI G'TCGA_C
Cuts at: 1936 5314 1936
Size: 3378 4594
SapI GCTCTTCn'nnn_
Cuts at: 2335 2947 4486 2335
Size: 612 1539 5821
Fragments arranged by size:
5821 1539 612
SbfI CC_TGCA'GG
Cuts at: 5324 5324
Size: 7972
ScaI AGT'ACT
Cuts at: 6220 7932 6220
Size: 1712 6260
SfcI C'TryA_G
Cuts at: 826 1045 2354 2736 3218 4206 4980 5320 6455
Size: 219 1309 382 482 988 774 340 1135
Cuts at: 6455 7136 7327 826
Size: 681 191 1471
Fragments arranged by size:
1471 1309 1135 988 774 681 482 382
340 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7972
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7972
SmaI CCC'GGG
Cuts at: 1872 5299 1872
Size: 3427 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3983 5163 5239 6076 6977 7239
Size: 197 926 2040 1180 76 837 901 262
Cuts at: 7239 820
Size: 1553
Fragments arranged by size:
2040 1553 1180 926 901 837 262 197
76
SnaBI TAC'GTA
Cuts at: 493 5763 493
Size: 5270 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7972
SphI G_CATG'C
Cuts at: 1132 4149 5330 1132
Size: 3017 1181 3774
Fragments arranged by size:
3774 3017 1181
SspI AAT'ATT
Cuts at: 5 52 2187 4265 5140 5896 5
Size: 47 2135 2078 875 756 2081
Fragments arranged by size:
2135 2081 2078 875 756 47
StuI AGG'CCT
Cuts at: 1517 2692 3236 4105 4432 1517
Size: 1175 544 869 327 5057
Fragments arranged by size:
5057 1175 869 544 327
StyI C'CwwG_G
Cuts at: 513 1065 2004 2927 3409 3738 4226 4548 5411
Size: 552 939 923 482 329 488 322 863
Cuts at: 5411 513
Size: 3074
Fragments arranged by size:
3074 939 923 863 552 488 482 329
322
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5999 6016 6169 6354 6799 325
Size: 1255 4419 17 153 185 445 1498
Fragments arranged by size:
4419 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3698 4153 5260
Size: 274 80 33 51 1443 1721 455 1107
Cuts at: 5260 6218 7930 96
Size: 958 1712 138
Fragments arranged by size:
1721 1712 1443 1107 958 455 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3330 3362 4466 4498 7932 7964 3330
Size: 32 1104 32 3434 32 3338
Fragments arranged by size:
3434 3338 1104 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4326 4697 1316
Size: 3010 371 4591
Fragments arranged by size:
4591 3010 371
XbaI T'CTAG_A
Cuts at: 5308 5308
Size: 7972
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3877 3877
Size: 7972
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7972
XmaI C'CCGG_G
Cuts at: 1870 5297 1870
Size: 3427 4545
XmnI GAAnn'nnTTC
Cuts at: 3714 5139 6101 3714
Size: 1425 962 5585
Fragments arranged by size:
5585 1425 962
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5780 276
Size: 53 83 186 936 4246 2468
Fragments arranged by size:
4246 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BsiWI BsmBI BstXI BstZ17I FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PspOMI PspXI PsrI
RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI