Restriction map for
pFN21AA1385
(Circular) (Six-base) MAPSORT of: pFN21AA1385.seq Check: 4117 from: 1 to: 6913
pFN21AA1385 6913 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3745 3914 3745
Size: 169 6744
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4723 278
Size: 53 83 186 936 3187 2468
Fragments arranged by size:
3187 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4033 4256 1937
Size: 2096 223 4594
Fragments arranged by size:
4594 2096 223
Acc65I G'GTAC_C
Cuts at: 4234 4234
Size: 6913
AclI AA'CG_TT
Cuts at: 1563 5040 5413 1563
Size: 3477 373 3063
Fragments arranged by size:
3477 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2068 2790 3345 3564 4976 6360 6628 1632
Size: 436 722 555 219 1412 1384 268 1917
Fragments arranged by size:
1917 1412 1384 722 555 436 268 219
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6716
AflIII A'CryG_T
Cuts at: 1203 5812 6789 1203
Size: 4609 977 1327
Fragments arranged by size:
4609 1327 977
AgeI A'CCGG_T
Cuts at: 5744 5744
Size: 6913
AhdI GACnn_n'nnGTC
Cuts at: 5642 5642
Size: 6913
AleI CACnn'nnGTG
Cuts at: 1166 2936 1166
Size: 1770 5143
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6913
AlwNI CAG_nnn'CTG
Cuts at: 3760 4656 6228 3760
Size: 896 1572 4445
Fragments arranged by size:
4445 1572 896
ApaI G_GGCC'C
Cuts at: 3440 3440
Size: 6913
ApaLI G'TGCA_C
Cuts at: 4970 6126 4970
Size: 1156 5757
ApoI r'AATT_y
Cuts at: 1493 3342 4222 4448 4600 6709 1493
Size: 1849 880 226 152 2109 1697
Fragments arranged by size:
2109 1849 1697 880 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5467 160
Size: 872 4435 1606
Fragments arranged by size:
4435 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6913
AvaI C'yCGr_G
Cuts at: 1870 1943 4238 1870
Size: 73 2295 4545
Fragments arranged by size:
4545 2295 73
BamHI G'GATC_C
Cuts at: 3955 4243 4610 3955
Size: 288 367 6258
Fragments arranged by size:
6258 367 288
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4234 5689 618
Size: 325 217 627 2447 1455 1842
Fragments arranged by size:
2447 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3440 4170 4232 1391
Size: 2049 730 62 4072
Fragments arranged by size:
4072 2049 730 62
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2106 2628 2953 3446 928
Size: 971 207 522 325 493 4395
Fragments arranged by size:
4395 971 522 493 325 207
BbvCI CC'TCA_GC
Cuts at: 2792 2792
Size: 6913
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5104 5138 1903
Size: 34 3167 34 3678
Fragments arranged by size:
3678 3167 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3736 4805 6015 3736
Size: 1069 1210 4634
Fragments arranged by size:
4634 1210 1069
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6913
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5524 136
Size: 107 122 71 631 605 3852 1525
Fragments arranged by size:
3852 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2125 6908 2125
Size: 4783 2130
BlpI GC'TnA_GC
Cuts at: 4330 4330
Size: 6913
Bme1580I G_kGCm'C
Cuts at: 1790 3440 4072 4974 6130 1790
Size: 1650 632 902 1156 2573
Fragments arranged by size:
2573 1650 1156 902 632
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6913
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4644 5602 442
Size: 416 53 83 399 24 3227 958 1753
Fragments arranged by size:
3227 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6913
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2277 2280 3026 5573 1239
Size: 1038 3 746 2547 2579
Fragments arranged by size:
2579 2547 1038 746 3
Bpu10I CC'TnA_GC
Cuts at: 2792 2823 2792
Size: 31 6882
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3057 3734 5038 5903 6201 3057
Size: 677 1304 865 298 3769
Fragments arranged by size:
3769 1304 865 677 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5576 882
Size: 617 4077 2219
Fragments arranged by size:
4077 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2513 4162 4704 493
Size: 711 1309 1649 542 2702
Fragments arranged by size:
2702 1649 1309 711 542
BsaBI GATnn'nnATC
Cuts at: 6907 6907
Size: 6913
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4720 5102 5737 275
Size: 53 83 186 936 3187 382 635 1451
Fragments arranged by size:
3187 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3016 5346 5744 6018 6165 6576 3016
Size: 2330 398 274 147 411 3353
Fragments arranged by size:
3353 2330 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2771 2801 3232 3262 1178
Size: 30 165 30 1368 30 431 30 4829
Fragments arranged by size:
4829 1368 431 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2580 1186
Size: 1394 5519
BseYI C'CCAG_C
Cuts at: 3109 6116 6612 3109
Size: 3007 496 3410
Fragments arranged by size:
3410 3007 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2902 3912 1163
Size: 573 1166 1010 4164
Fragments arranged by size:
4164 1166 1010 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2013 4363 5124 6152 1540
Size: 275 186 12 2350 761 1028 2301
Fragments arranged by size:
2350 2301 1028 761 275 186 12
BsiHKAI G_wGCw'C
Cuts at: 2772 2824 3250 4170 4974 5059 6130 2772
Size: 52 426 920 804 85 1071 3555
Fragments arranged by size:
3555 1071 920 804 426 85 52
BsmI GAATG_Cn'
Cuts at: 2468 4424 4517 2468
Size: 1956 93 4864
Fragments arranged by size:
4864 1956 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2772 2824 3250 3440 4072 4170 4232
Size: 399 982 52 426 190 632 98 62
Cuts at: 4232 4974 5059 6130 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 982 742 632 426 399 190
98 85 62 52
BspEI T'CCGG_A
Cuts at: 6576 6576
Size: 6913
BspHI T'CATG_A
Cuts at: 4802 6532 4802
Size: 1730 5183
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2442 3745 3914 4268 844
Size: 1598 1303 169 354 3489
Fragments arranged by size:
3489 1598 1303 354 169
BsrBI CCG'CTC
Cuts at: 1394 1823 4800 1394
Size: 429 2977 3507
Fragments arranged by size:
3507 2977 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2352 2721 5408 5582 65
Size: 1183 1104 369 2687 174 1396
Fragments arranged by size:
2687 1396 1183 1104 369 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5557 5744 1700
Size: 110 3747 187 2869
Fragments arranged by size:
3747 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6913
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6913
BssSI C'ACGA_G
Cuts at: 2280 2754 2775 2817 4973 5985 2280
Size: 474 21 42 2156 1012 3208
Fragments arranged by size:
3208 2156 1012 474 42 21
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2723 794
Size: 1929 4984
BstBI TT'CG_AA
Cuts at: 5717 5717
Size: 6913
BstEII G'GTnAC_C
Cuts at: 1184 3617 3901 1184
Size: 2433 284 4196
Fragments arranged by size:
4196 2433 284
BstXI CCAn_nnnn'nTGG
Cuts at: 4065 4065
Size: 6913
BstYI r'GATC_y
Cuts at: 1972 2125 2137 2453 3783 3955 4243 4610 4995
Size: 153 12 316 1330 172 288 367 385
Cuts at: 4995 5012 6464 6550 6562 6908 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1330 385 367 346 316 288
172 153 86 17 12 12
BstZ17I GTA'TAC
Cuts at: 4034 4034
Size: 6913
BtgI C'CryG_G
Cuts at: 513 1065 2004 3729 513
Size: 552 939 1725 3697
Fragments arranged by size:
3697 1725 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2936 487
Size: 683 56 1710 4464
Fragments arranged by size:
4464 1710 683 56
BtsI GCAGTG_nn'
Cuts at: 2113 4123 4429 5223 5243 2113
Size: 2010 306 794 20 3783
Fragments arranged by size:
3783 2010 794 306 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6913
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6878
DraI TTT'AAA
Cuts at: 4217 4217
Size: 6913
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5920 809
Size: 507 4604 1802
Fragments arranged by size:
4604 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3359 3385 3417
Size: 54 1425 50 275 18 1529 26 32
Cuts at: 3417 3962 4360 5249 6878 8
Size: 545 398 889 1629 43
Fragments arranged by size:
1629 1529 1425 889 545 398 275 54
50 43 32 26 18
EagI C'GGCC_G
Cuts at: 1537 1812 4360 1537
Size: 275 2548 4090
Fragments arranged by size:
4090 2548 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2640 2649 3597 4843 1353
Size: 1287 9 948 1246 3423
Fragments arranged by size:
3423 1287 1246 948 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5500 5874 6020 387
Size: 1064 138 3911 374 146 1280
Fragments arranged by size:
3911 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4168 4168
Size: 6913
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2068 2277 2280 2790 3026 3345 3564
Size: 393 436 209 3 510 236 319 219
Cuts at: 3564 4976 5573 6360 6628 1239
Size: 1412 597 787 268 1524
Fragments arranged by size:
1524 1412 787 597 510 436 393 319
268 236 219 209 3
EcoO109I rG'GnC_Cy
Cuts at: 3437 3437
Size: 6913
EcoRI G'AATT_C
Cuts at: 3342 4222 4600 3342
Size: 880 378 5655
Fragments arranged by size:
5655 880 378
EcoRV GAT'ATC
Cuts at: 2929 3373 2929
Size: 444 6469
FspI TGC'GCA
Cuts at: 4115 5419 4115
Size: 1304 5609
HaeII r_GCGC'y
Cuts at: 1678 6060 1678
Size: 4382 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2681 2713 2771 2803 4084
Size: 32 135 32 1109 32 58 32 1281
Cuts at: 4084 4116 4126 4158 5554 5586 5628 5660 1373
Size: 32 10 32 1396 32 42 32 2626
Fragments arranged by size:
2626 1396 1281 1109 135 58 42 32
32 32 32 32 32 32 32 10
HincII GTy'rAC
Cuts at: 669 1657 1938 3519 4257 4503 669
Size: 988 281 1581 738 246 3079
Fragments arranged by size:
3079 1581 988 738 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6913
HpaI GTT'AAC
Cuts at: 4503 4503
Size: 6913
KpnI G_GTAC'C
Cuts at: 4238 4238
Size: 6913
MfeI C'AATT_G
Cuts at: 4512 4512
Size: 6913
MluI A'CGCG_T
Cuts at: 6789 6789
Size: 6913
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2035 6027 6211 1305
Size: 730 3992 184 2007
Fragments arranged by size:
3992 2007 730 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3419 10
Size: 54 1425 343 1587 3504
Fragments arranged by size:
3504 1587 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2936 4871 5230 5389 518
Size: 648 1770 1935 359 159 2042
Fragments arranged by size:
2042 1935 1770 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4329 5006 6154 6399 1606
Size: 2723 677 1148 245 2120
Fragments arranged by size:
2723 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3729 513
Size: 552 939 1725 3697
Fragments arranged by size:
3697 1725 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6913
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6913
NotI GC'GGCC_GC
Cuts at: 4360 4360
Size: 6913
NspI r_CATG'y
Cuts at: 1132 1229 2386 2959 4114 4271 5816 1132
Size: 97 1157 573 1155 157 1545 2229
Fragments arranged by size:
2229 1545 1157 1155 573 157 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6913
PciI A'CATG_T
Cuts at: 5812 5812
Size: 6913
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6913
PfoI T'CCnGG_A
Cuts at: 3003 3003
Size: 6913
PmeI GTTT'AAAC
Cuts at: 4217 4217
Size: 6913
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4982 5014 6495 6527 4982
Size: 32 1481 32 5368
Fragments arranged by size:
5368 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6913
PsiI TTA'TAA
Cuts at: 4483 4483
Size: 6913
PspOMI G'GGCC_C
Cuts at: 3436 3436
Size: 6913
PstI C_TGCA'G
Cuts at: 830 2716 4265 830
Size: 1886 1549 3478
Fragments arranged by size:
3478 1886 1549
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6913
SacI G_AGCT'C
Cuts at: 4170 4170
Size: 6913
SalI G'TCGA_C
Cuts at: 1936 4255 1936
Size: 2319 4594
SbfI CC_TGCA'GG
Cuts at: 4265 4265
Size: 6913
ScaI AGT'ACT
Cuts at: 5161 6873 5161
Size: 1712 5201
SfcI C'TryA_G
Cuts at: 826 1045 2712 2844 3465 4261 5396 6077 6268
Size: 219 1667 132 621 796 1135 681 191
Cuts at: 6268 826
Size: 1471
Fragments arranged by size:
1667 1471 1135 796 681 621 219 191
132
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6913
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6913
SmaI CCC'GGG
Cuts at: 1872 4240 1872
Size: 2368 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3072 3749 5017 5918 6180 820
Size: 197 926 1129 677 1268 901 262 1553
Fragments arranged by size:
1553 1268 1129 926 901 677 262 197
SnaBI TAC'GTA
Cuts at: 493 4704 493
Size: 4211 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6913
SphI G_CATG'C
Cuts at: 1132 2386 4114 4271 1132
Size: 1254 1728 157 3774
Fragments arranged by size:
3774 1728 1254 157
SspI AAT'ATT
Cuts at: 5 52 4837 5
Size: 47 4785 2081
Fragments arranged by size:
4785 2081 47
StuI AGG'CCT
Cuts at: 1517 2395 1517
Size: 878 6035
StyI C'CwwG_G
Cuts at: 513 1065 2004 2934 3729 4064 4352 513
Size: 552 939 930 795 335 288 3074
Fragments arranged by size:
3074 939 930 795 552 335 288
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2027 2141 4940 4957 5110 5295 5740
Size: 1255 447 114 2799 17 153 185 445
Cuts at: 5740 325
Size: 1498
Fragments arranged by size:
2799 1498 1255 447 445 185 153 114
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3277 4076 5159
Size: 274 80 33 51 1443 1300 799 1083
Cuts at: 5159 6871 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1300 1083 799 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2853 2885 6873 6905 2853
Size: 32 3988 32 2861
Fragments arranged by size:
3988 2861 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6913
XbaI T'CTAG_A
Cuts at: 4249 4249
Size: 6913
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3631 3631
Size: 6913
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6913
XmaI C'CCGG_G
Cuts at: 1870 4238 1870
Size: 2368 4545
XmnI GAAnn'nnTTC
Cuts at: 2466 5042 2466
Size: 2576 4337
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4721 276
Size: 53 83 186 936 3187 2468
Fragments arranged by size:
3187 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI Bsu36I DraIII EcoNI FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PpuMI PspXI PsrI PvuII RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI