Restriction map for
pFN21AA1384
(Circular) (Six-base) MAPSORT of: pFN21AA1384.seq Check: 8734 from: 1 to: 6589
pFN21AA1384 6589 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2557 2557
Size: 6589
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4399 278
Size: 53 83 186 936 2863 2468
Fragments arranged by size:
2863 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3932 1937
Size: 1995 4594
Acc65I G'GTAC_C
Cuts at: 3910 3910
Size: 6589
AclI AA'CG_TT
Cuts at: 1563 4716 5089 1563
Size: 3153 373 3063
Fragments arranged by size:
3153 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2902 4652 6036 6304 1632
Size: 1270 1750 1384 268 1917
Fragments arranged by size:
1917 1750 1384 1270 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6392
AflIII A'CryG_T
Cuts at: 1203 5488 6465 1203
Size: 4285 977 1327
Fragments arranged by size:
4285 1327 977
AgeI A'CCGG_T
Cuts at: 5420 5420
Size: 6589
AhdI GACnn_n'nnGTC
Cuts at: 5318 5318
Size: 6589
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6589
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2510 1184
Size: 1326 5263
AlwNI CAG_nnn'CTG
Cuts at: 2881 2941 3385 3842 4332 5904 2881
Size: 60 444 457 490 1572 3566
Fragments arranged by size:
3566 1572 490 457 444 60
ApaI G_GGCC'C
Cuts at: 2245 2245
Size: 6589
ApaLI G'TGCA_C
Cuts at: 3407 4646 5802 3407
Size: 1239 1156 4194
Fragments arranged by size:
4194 1239 1156
ApoI r'AATT_y
Cuts at: 1493 2952 3178 3898 4124 4276 6385 1493
Size: 1459 226 720 226 152 2109 1697
Fragments arranged by size:
2109 1697 1459 720 226 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5143 160
Size: 872 4111 1606
Fragments arranged by size:
4111 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6589
AvaI C'yCGr_G
Cuts at: 1870 1943 2207 2315 2361 2420 3914 1870
Size: 73 264 108 46 59 1494 4545
Fragments arranged by size:
4545 1494 264 108 73 59 46
BamHI G'GATC_C
Cuts at: 3919 4286 3919
Size: 367 6222
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3910 5365 618
Size: 325 217 627 2123 1455 1842
Fragments arranged by size:
2123 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2245 2316 3502 3908 1391
Size: 854 71 1186 406 4072
Fragments arranged by size:
4072 1186 854 406 71
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3059 928
Size: 971 1160 4458
Fragments arranged by size:
4458 1160 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4780 4814 1903
Size: 34 2843 34 3678
Fragments arranged by size:
3678 2843 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4481 5691 4481
Size: 1210 5379
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6589
BfrBI ATG'CAT
Cuts at: 3260 3260
Size: 6589
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2156 2232 2920
Size: 107 122 71 631 605 484 76 688
Cuts at: 2920 5200 136
Size: 2280 1525
Fragments arranged by size:
2280 1525 688 631 605 484 122 107
76 71
BglII A'GATC_T
Cuts at: 2544 6584 2544
Size: 4040 2549
BlpI GC'TnA_GC
Cuts at: 4006 4006
Size: 6589
Bme1580I G_kGCm'C
Cuts at: 1790 2245 3094 3411 4650 5806 1790
Size: 455 849 317 1239 1156 2573
Fragments arranged by size:
2573 1239 1156 849 455 317
BmgBI CAC'GTC
Cuts at: 1318 2107 1318
Size: 789 5800
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3476 4320 5278
Size: 416 53 83 399 24 2059 844 958
Cuts at: 5278 442
Size: 1753
Fragments arranged by size:
2059 1753 958 844 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6589
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2176 2618 2762 3763 5249 1239
Size: 937 442 144 1001 1486 2579
Fragments arranged by size:
2579 1486 1001 937 442 144
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4714 5579 5877 4714
Size: 865 298 5426
Fragments arranged by size:
5426 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2597 2750 5252 882
Size: 617 1098 153 2502 2219
Fragments arranged by size:
2502 2219 1098 617 153
BsaAI yAC'GTr
Cuts at: 493 1204 3286 4380 493
Size: 711 2082 1094 2702
Fragments arranged by size:
2702 2082 1094 711
BsaBI GATnn'nnATC
Cuts at: 6583 6583
Size: 6589
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2892 4396 4778 5413
Size: 53 83 186 936 1359 1504 382 635
Cuts at: 5413 275
Size: 1451
Fragments arranged by size:
1504 1451 1359 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3004 5022 5420 5694 5841 6252 3004
Size: 2018 398 274 147 411 3341
Fragments arranged by size:
3341 2018 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2012 2042 2462 2492 3441
Size: 30 165 30 609 30 420 30 949
Cuts at: 3441 3471 1178
Size: 30 4296
Fragments arranged by size:
4296 949 609 420 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2324 2483 2615 2675 1186
Size: 1138 159 132 60 5100
Fragments arranged by size:
5100 1138 159 132 60
BseYI C'CCAG_C
Cuts at: 2042 3017 5792 6288 2042
Size: 975 2775 496 2343
Fragments arranged by size:
2775 2343 975 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2048 2480 2546 2573 3720 1163
Size: 573 312 432 66 27 1147 4032
Fragments arranged by size:
4032 1147 573 432 312 66 27
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2183 2752 4039 4800 5828 1540
Size: 275 186 182 569 1287 761 1028 2301
Fragments arranged by size:
2301 1287 1028 761 569 275 186 182
BsiHKAI G_wGCw'C
Cuts at: 2421 2748 3411 4650 4735 5806 2421
Size: 327 663 1239 85 1071 3204
Fragments arranged by size:
3204 1239 1071 663 327 85
BsmI GAATG_Cn'
Cuts at: 3629 4100 4193 3629
Size: 471 93 6025
Fragments arranged by size:
6025 471 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2245 2316 2421 2748 3094 3411 3502
Size: 399 455 71 105 327 346 317 91
Cuts at: 3502 3908 4650 4735 5806 1391
Size: 406 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 455 406 399 346 327
317 105 91 85 71
BspEI T'CCGG_A
Cuts at: 6252 6252
Size: 6589
BspHI T'CATG_A
Cuts at: 2784 4478 6208 2784
Size: 1694 1730 3165
Fragments arranged by size:
3165 1730 1694
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2067 2085 2557 3944 844
Size: 1223 18 472 1387 3489
Fragments arranged by size:
3489 1387 1223 472 18
BsrBI CCG'CTC
Cuts at: 1394 1823 2833 4476 1394
Size: 429 1010 1643 3507
Fragments arranged by size:
3507 1643 1010 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2137 5084 5258 65
Size: 1183 889 2947 174 1396
Fragments arranged by size:
2947 1396 1183 889 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3009 5233 5420 1700
Size: 110 1199 2224 187 2869
Fragments arranged by size:
2869 2224 1199 187 110
BsrGI T'GTAC_A
Cuts at: 96 3439 96
Size: 3343 3246
BssHII G'CGCG_C
Cuts at: 1925 2668 1925
Size: 743 5846
BssSI C'ACGA_G
Cuts at: 4649 5661 4649
Size: 1012 5577
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3842 794
Size: 3048 3541
BstBI TT'CG_AA
Cuts at: 5393 5393
Size: 6589
BstEII G'GTnAC_C
Cuts at: 1184 2509 3641 1184
Size: 1325 1132 4132
Fragments arranged by size:
4132 1325 1132
BstXI CCAn_nnnn'nTGG
Cuts at: 2614 3025 2614
Size: 411 6178
BstYI r'GATC_y
Cuts at: 1972 2012 2490 2544 3919 4286 4671 4688 6140
Size: 40 478 54 1375 367 385 17 1452
Cuts at: 6140 6226 6238 6584 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1375 478 385 367 346 86
54 40 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2697 3461 513
Size: 552 1632 764 3641
Fragments arranged by size:
3641 1632 764 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5850
Fragments arranged by size:
5850 683 56
BtsI GCAGTG_nn'
Cuts at: 2926 3097 3411 3708 4105 4899 4919 2926
Size: 171 314 297 397 794 20 4596
Fragments arranged by size:
4596 794 397 314 297 171 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6589
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6554
DraI TTT'AAA
Cuts at: 3893 3893
Size: 6589
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5596 809
Size: 507 4280 1802
Fragments arranged by size:
4280 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2224 2436 2616
Size: 54 1425 50 275 18 394 212 180
Cuts at: 2616 2943 4036 4925 6554 8
Size: 327 1093 889 1629 43
Fragments arranged by size:
1629 1425 1093 889 394 327 275 212
180 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4036 1537
Size: 275 2224 4090
Fragments arranged by size:
4090 2224 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2192 2723 3238 4519 1353
Size: 839 531 515 1281 3423
Fragments arranged by size:
3423 1281 839 531 515
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5176 5550 5696 387
Size: 1064 138 3587 374 146 1280
Fragments arranged by size:
3587 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2176 2618 2762 2902 3763 4652 5249
Size: 393 544 442 144 140 861 889 597
Cuts at: 5249 6036 6304 1239
Size: 787 268 1524
Fragments arranged by size:
1524 889 861 787 597 544 442 393
268 144 140
EcoNI CCTnn'n_nnAGG
Cuts at: 2633 2633
Size: 6589
EcoO109I rG'GnC_Cy
Cuts at: 2025 2241 2242 3753 3839 2025
Size: 216 1 1511 86 4775
Fragments arranged by size:
4775 1511 216 86 1
EcoRI G'AATT_C
Cuts at: 2952 3898 4276 2952
Size: 946 378 5265
Fragments arranged by size:
5265 946 378
FspI TGC'GCA
Cuts at: 5095 5095
Size: 6589
HaeII r_GCGC'y
Cuts at: 1678 2717 2794 3361 5736 1678
Size: 1039 77 567 2375 2531
Fragments arranged by size:
2531 2375 1039 567 77
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2012 2044 2462 2494 2531
Size: 32 135 32 440 32 418 32 37
Cuts at: 2531 2563 3441 3473 5230 5262 5304 5336 1373
Size: 32 878 32 1757 32 42 32 2626
Fragments arranged by size:
2626 1757 878 440 418 135 42 37
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3933 4179 669
Size: 988 281 1995 246 3079
Fragments arranged by size:
3079 1995 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6589
HpaI GTT'AAC
Cuts at: 4179 4179
Size: 6589
KpnI G_GTAC'C
Cuts at: 3914 3914
Size: 6589
MfeI C'AATT_G
Cuts at: 3570 4188 3570
Size: 618 5971
MluI A'CGCG_T
Cuts at: 6465 6465
Size: 6589
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2685 2965 3249 5703 5887 1305
Size: 1380 280 284 2454 184 2007
Fragments arranged by size:
2454 2007 1380 284 280 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2618 2945 10
Size: 54 1425 343 786 327 3654
Fragments arranged by size:
3654 1425 786 343 327 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3074 3606 4547 4906 5065 518
Size: 648 1908 532 941 359 159 2042
Fragments arranged by size:
2042 1908 941 648 532 359 159
MspA1I CmG'CkG
Cuts at: 1606 2095 3741 4005 4682 5830 6075 1606
Size: 489 1646 264 677 1148 245 2120
Fragments arranged by size:
2120 1646 1148 677 489 264 245
NcoI C'CATG_G
Cuts at: 513 1065 3461 513
Size: 552 2396 3641
Fragments arranged by size:
3641 2396 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6589
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6589
NotI GC'GGCC_GC
Cuts at: 4036 4036
Size: 6589
NsiI A_TGCA'T
Cuts at: 3262 3262
Size: 6589
NspI r_CATG'y
Cuts at: 1132 1229 2767 3264 3947 5492 1132
Size: 97 1538 497 683 1545 2229
Fragments arranged by size:
2229 1545 1538 683 497 97
PasI CC'CwG_GG
Cuts at: 1348 2122 1348
Size: 774 5815
PciI A'CATG_T
Cuts at: 5488 5488
Size: 6589
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2845 1108
Size: 1737 4852
PfoI T'CCnGG_A
Cuts at: 2998 2998
Size: 6589
PmeI GTTT'AAAC
Cuts at: 3893 3893
Size: 6589
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4658 4690 6171 6203 4658
Size: 32 1481 32 5044
Fragments arranged by size:
5044 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2025 2025
Size: 6589
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6589
PsiI TTA'TAA
Cuts at: 4159 4159
Size: 6589
PspOMI G'GGCC_C
Cuts at: 2241 2241
Size: 6589
PspXI vC'TCGA_Gb
Cuts at: 2420 2420
Size: 6589
PstI C_TGCA'G
Cuts at: 830 2388 2933 3941 830
Size: 1558 545 1008 3478
Fragments arranged by size:
3478 1558 1008 545
PvuI CG_AT'CG
Cuts at: 2001 2183 2001
Size: 182 6407
PvuII CAG'CTG
Cuts at: 2095 3741 2095
Size: 1646 4943
RsrII CG'GwC_CG
Cuts at: 3007 3007
Size: 6589
SalI G'TCGA_C
Cuts at: 1936 3931 1936
Size: 1995 4594
SapI GCTCTTCn'nnn_
Cuts at: 2192 2723 3238 2192
Size: 531 515 5543
Fragments arranged by size:
5543 531 515
SbfI CC_TGCA'GG
Cuts at: 3941 3941
Size: 6589
ScaI AGT'ACT
Cuts at: 2174 4837 6549 2174
Size: 2663 1712 2214
Fragments arranged by size:
2663 2214 1712
SexAI A'CCwGG_T
Cuts at: 2376 2628 2376
Size: 252 6337
SfcI C'TryA_G
Cuts at: 826 1045 2384 2929 3736 3937 5072 5753 5944
Size: 219 1339 545 807 201 1135 681 191
Cuts at: 5944 826
Size: 1471
Fragments arranged by size:
1471 1339 1135 807 681 545 219 201
191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2156 2232 2156
Size: 76 6513
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6589
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6589
SmaI CCC'GGG
Cuts at: 1872 2317 2363 3916 1872
Size: 445 46 1553 4545
Fragments arranged by size:
4545 1553 445 46
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2420 4693 5594 5856 820
Size: 197 926 477 2273 901 262 1553
Fragments arranged by size:
2273 1553 926 901 477 262 197
SnaBI TAC'GTA
Cuts at: 493 3286 4380 493
Size: 2793 1094 2702
Fragments arranged by size:
2793 2702 1094
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6589
SphI G_CATG'C
Cuts at: 1132 2767 3947 1132
Size: 1635 1180 3774
Fragments arranged by size:
3774 1635 1180
SspI AAT'ATT
Cuts at: 5 52 4513 5
Size: 47 4461 2081
Fragments arranged by size:
4461 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6589
StyI C'CwwG_G
Cuts at: 513 1065 2247 3461 4028 513
Size: 552 1182 1214 567 3074
Fragments arranged by size:
3074 1214 1182 567 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2470 3125 3244 4616 4633 4786 4971
Size: 1255 890 655 119 1372 17 153 185
Cuts at: 4971 5416 325
Size: 445 1498
Fragments arranged by size:
1498 1372 1255 890 655 445 185 153
119 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2172 3173 3439
Size: 274 80 33 51 1443 195 1001 266
Cuts at: 3439 4835 6547 96
Size: 1396 1712 138
Fragments arranged by size:
1712 1443 1396 1001 274 266 195 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6549 6581 6549
Size: 32 6557
Tth111I GACn'n_nGTC
Cuts at: 1316 2449 2867 1316
Size: 1133 418 5038
Fragments arranged by size:
5038 1133 418
XbaI T'CTAG_A
Cuts at: 3925 3925
Size: 6589
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2374 2626 2374
Size: 252 6337
XhoI C'TCGA_G
Cuts at: 1943 2420 1943
Size: 477 6112
XmaI C'CCGG_G
Cuts at: 1870 2315 2361 3914 1870
Size: 445 46 1553 4545
Fragments arranged by size:
4545 1553 445 46
XmnI GAAnn'nnTTC
Cuts at: 3593 4718 3593
Size: 1125 5464
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4397 276
Size: 53 83 186 936 2863 2468
Fragments arranged by size:
2863 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII
SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PsrI SacI SacII SanDI SfoI SrfI
SwaI