Restriction map for
pFN21AA1381
(Circular) (Six-base) MAPSORT of: pFN21AA1381.seq Check: 5196 from: 1 to: 7645
pFN21AA1381 7645 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2530 2982 2530
Size: 452 7193
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5455 278
Size: 53 83 186 936 3919 2468
Fragments arranged by size:
3919 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4988 1937
Size: 3051 4594
Acc65I G'GTAC_C
Cuts at: 2169 4966 2169
Size: 2797 4848
AclI AA'CG_TT
Cuts at: 1563 5772 6145 1563
Size: 4209 373 3063
Fragments arranged by size:
4209 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2054 2822 3599 3908 5708 7092 7360 1632
Size: 422 768 777 309 1800 1384 268 1917
Fragments arranged by size:
1917 1800 1384 777 768 422 309 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7448
AflIII A'CryG_T
Cuts at: 1203 3072 3414 4893 6544 7521 1203
Size: 1869 342 1479 1651 977 1327
Fragments arranged by size:
1869 1651 1479 1327 977 342
AgeI A'CCGG_T
Cuts at: 6476 6476
Size: 7645
AhdI GACnn_n'nnGTC
Cuts at: 4874 4894 6374 4874
Size: 20 1480 6145
Fragments arranged by size:
6145 1480 20
AleI CACnn'nnGTG
Cuts at: 1166 2745 1166
Size: 1579 6066
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2609 2829 3822 1184
Size: 1425 220 993 5007
Fragments arranged by size:
5007 1425 993 220
AlwNI CAG_nnn'CTG
Cuts at: 3265 3289 3855 3865 4184 4853 5388 6960 3265
Size: 24 566 10 319 669 535 1572 3950
Fragments arranged by size:
3950 1572 669 566 535 319 24 10
ApaI G_GGCC'C
Cuts at: 2314 2314
Size: 7645
ApaLI G'TGCA_C
Cuts at: 5702 6858 5702
Size: 1156 6489
ApoI r'AATT_y
Cuts at: 1493 2382 4164 4954 5180 5332 7441 1493
Size: 889 1782 790 226 152 2109 1697
Fragments arranged by size:
2109 1782 1697 889 790 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4272 6199 160
Size: 872 3240 1927 1606
Fragments arranged by size:
3240 1927 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7645
AvaI C'yCGr_G
Cuts at: 1870 1943 4970 1870
Size: 73 3027 4545
Fragments arranged by size:
4545 3027 73
BamHI G'GATC_C
Cuts at: 3066 4975 5342 3066
Size: 1909 367 5369
Fragments arranged by size:
5369 1909 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2169 4966 6421 618
Size: 325 217 627 382 2797 1455 1842
Fragments arranged by size:
2797 1842 1455 627 382 325 217
BanII G_rGCy'C
Cuts at: 1391 2314 2405 3336 3920 3949 4964 1391
Size: 923 91 931 584 29 1015 4072
Fragments arranged by size:
4072 1015 931 923 584 91 29
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3090 4893 928
Size: 971 1191 1803 3680
Fragments arranged by size:
3680 1803 1191 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5836 5870 1903
Size: 34 3899 34 3678
Fragments arranged by size:
3899 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5537 6747 5537
Size: 1210 6435
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7645
BfrBI ATG'CAT
Cuts at: 3735 3735
Size: 7645
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2015 2246 2283
Size: 107 122 71 631 605 343 231 37
Cuts at: 2283 2288 2708 6256 136
Size: 5 420 3548 1525
Fragments arranged by size:
3548 1525 631 605 420 343 231 122
107 71 37 5
BglII A'GATC_T
Cuts at: 2394 7640 2394
Size: 5246 2399
BlpI GC'TnA_GC
Cuts at: 5062 5062
Size: 7645
Bme1580I G_kGCm'C
Cuts at: 1790 2314 2927 3881 5706 6862 1790
Size: 524 613 954 1825 1156 2573
Fragments arranged by size:
2573 1825 1156 954 613 524
BmgBI CAC'GTC
Cuts at: 1318 4896 1318
Size: 3578 4067
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4093 5376 6334
Size: 416 53 83 399 24 2676 1283 958
Cuts at: 6334 442
Size: 1753
Fragments arranged by size:
2676 1753 1283 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7645
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2688 4381 2688
Size: 1693 5952
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2123 2419 2459 2909 3245 3301 4013 4078
Size: 884 296 40 450 336 56 712 65
Cuts at: 4078 4495 6305 1239
Size: 417 1810 2579
Fragments arranged by size:
2579 1810 884 712 450 417 336 296
65 56 40
Bpu10I CC'TnA_GC
Cuts at: 4806 4806
Size: 7645
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5770 6635 6933 5770
Size: 865 298 6482
Fragments arranged by size:
6482 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2885 3143 6308 882
Size: 617 1386 258 3165 2219
Fragments arranged by size:
3165 2219 1386 617 258
BsaAI yAC'GTr
Cuts at: 493 1204 3121 5436 493
Size: 711 1917 2315 2702
Fragments arranged by size:
2702 2315 1917 711
BsaBI GATnn'nnATC
Cuts at: 7639 7639
Size: 7645
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2238 3156 5452 5834
Size: 53 83 186 936 705 918 2296 382
Cuts at: 5834 6469 275
Size: 635 1451
Fragments arranged by size:
2296 1451 936 918 705 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2386 2562 6078 6476 6750 6897 7308 2386
Size: 176 3516 398 274 147 411 2723
Fragments arranged by size:
3516 2723 411 398 274 176 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3299 3329 4166 4196 1178
Size: 30 165 30 1896 30 837 30 4627
Fragments arranged by size:
4627 1896 837 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2102 2156 2661 4016 4187 1186
Size: 916 54 505 1355 171 4644
Fragments arranged by size:
4644 1355 916 505 171 54
BseYI C'CCAG_C
Cuts at: 2313 3198 4149 6848 7344 2313
Size: 885 951 2699 496 2614
Fragments arranged by size:
2699 2614 951 885 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3722 3812 4086 4203 4631 4884 1163
Size: 573 1986 90 274 117 428 253 3924
Fragments arranged by size:
3924 1986 573 428 274 253 117 90
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2193 2292 5095 5856 6884 1540
Size: 275 186 192 99 2803 761 1028 2301
Fragments arranged by size:
2803 2301 1028 761 275 192 186 99
BsiHKAI G_wGCw'C
Cuts at: 2133 2405 2645 3336 3920 5706 5791 6862 2133
Size: 272 240 691 584 1786 85 1071 2916
Fragments arranged by size:
2916 1786 1071 691 584 272 240 85
BsmI GAATG_Cn'
Cuts at: 5156 5249 5156
Size: 93 7552
BsmBI CGTCTCn'nnnn_
Cuts at: 2066 2066
Size: 7645
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2133 2314 2405 2645 2927 3336 3881
Size: 399 343 181 91 240 282 409 545
Cuts at: 3881 3920 3949 4964 5706 5791 6862 1391
Size: 39 29 1015 742 85 1071 2174
Fragments arranged by size:
2174 1071 1015 742 545 409 399 343
282 240 181 91 85 39 29
BspEI T'CCGG_A
Cuts at: 2386 7308 2386
Size: 4922 2723
BspHI T'CATG_A
Cuts at: 5534 7264 5534
Size: 1730 5915
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2454 2530 2982 3117 3459 5000 844
Size: 1610 76 452 135 342 1541 3489
Fragments arranged by size:
3489 1610 1541 452 342 135 76
BsrBI CCG'CTC
Cuts at: 1394 1823 2084 3235 5532 1394
Size: 429 261 1151 2297 3507
Fragments arranged by size:
3507 2297 1151 429 261
BsrDI GCAATG_nn'
Cuts at: 65 1248 6140 6314 65
Size: 1183 4892 174 1396
Fragments arranged by size:
4892 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2282 6289 6476 1700
Size: 110 472 4007 187 2869
Fragments arranged by size:
4007 2869 472 187 110
BsrGI T'GTAC_A
Cuts at: 96 3544 4237 96
Size: 3448 693 3504
Fragments arranged by size:
3504 3448 693
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7645
BssSI C'ACGA_G
Cuts at: 5705 6717 5705
Size: 1012 6633
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3709 794
Size: 2915 4730
BstBI TT'CG_AA
Cuts at: 6449 6449
Size: 7645
BstEII G'GTnAC_C
Cuts at: 1184 4838 1184
Size: 3654 3991
BstXI CCAn_nnnn'nTGG
Cuts at: 2750 2750
Size: 7645
BstYI r'GATC_y
Cuts at: 1972 2044 2091 2394 2851 3066 4206 4975 5342
Size: 72 47 303 457 215 1140 769 367
Cuts at: 5342 5727 5744 7196 7282 7294 7640 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1140 769 457 385 367 346
303 215 86 72 47 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2013 2095 2223 2306 2743 3726
Size: 552 939 9 82 128 83 437 983
Cuts at: 3726 513
Size: 4432
Fragments arranged by size:
4432 983 939 552 437 128 83 82
9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4586 487
Size: 683 56 3360 3546
Fragments arranged by size:
3546 3360 683 56
BtsI GCAGTG_nn'
Cuts at: 3629 3899 4069 4198 5161 5955 5975 3629
Size: 270 170 129 963 794 20 5299
Fragments arranged by size:
5299 963 794 270 170 129 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7645
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7610
DraI TTT'AAA
Cuts at: 4949 4949
Size: 7645
DraIII CAC_nnn'GTG
Cuts at: 3748 3748
Size: 7645
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6652 809
Size: 507 5336 1802
Fragments arranged by size:
5336 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2190 2203
Size: 54 1425 50 275 18 177 183 13
Cuts at: 2203 2289 2300 2628 2700 2790 3846 4134 4492
Size: 86 11 328 72 90 1056 288 358
Cuts at: 4492 5092 5981 7610 8
Size: 600 889 1629 43
Fragments arranged by size:
1629 1425 1056 889 600 358 328 288
275 183 177 90 86 72 54 50
43 18 13 11
EagI C'GGCC_G
Cuts at: 1537 1812 2190 2289 5092 1537
Size: 275 378 99 2803 4090
Fragments arranged by size:
4090 2803 378 275 99
EarI CTCTTCn'nnn_
Cuts at: 1353 2655 2882 2937 3119 3428 3777 4481 5575
Size: 1302 227 55 182 309 349 704 1094
Cuts at: 5575 1353
Size: 3423
Fragments arranged by size:
3423 1302 1094 704 349 309 227 182
55
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2264 6232 6606 6752 387
Size: 1064 138 675 3968 374 146 1280
Fragments arranged by size:
3968 1280 1064 675 374 146 138
EcoICRI GAG'CTC
Cuts at: 2403 3334 3918 2403
Size: 931 584 6130
Fragments arranged by size:
6130 931 584
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2054 2123 2419 2459 2822 2909 3245
Size: 393 422 69 296 40 363 87 336
Cuts at: 3245 3301 3599 3908 4013 4078 4495 5708 6305
Size: 56 298 309 105 65 417 1213 597
Cuts at: 6305 7092 7360 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1213 787 597 422 417 393 363
336 309 298 296 268 105 87 69
65 56 40
EcoNI CCTnn'n_nnAGG
Cuts at: 2183 2276 2183
Size: 93 7552
EcoO109I rG'GnC_Cy
Cuts at: 2635 2635
Size: 7645
EcoRI G'AATT_C
Cuts at: 4954 5332 4954
Size: 378 7267
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2392 3253 3352 2392
Size: 861 99 6685
Fragments arranged by size:
6685 861 99
FspI TGC'GCA
Cuts at: 6151 6151
Size: 7645
HaeII r_GCGC'y
Cuts at: 1678 2110 4424 6792 1678
Size: 432 2314 2368 2531
Fragments arranged by size:
2531 2368 2314 432
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3299 3331 3611 3643 4109
Size: 32 135 32 1727 32 280 32 466
Cuts at: 4109 4141 4166 4198 4750 4782 4860 4880 4892
Size: 32 25 32 552 32 78 20 12
Cuts at: 4892 4912 6286 6318 6360 6392 1373
Size: 20 1374 32 42 32 2626
Fragments arranged by size:
2626 1727 1374 552 466 280 135 78
42 32 32 32 32 32 32 32
32 32 25 20 20 12
HincII GTy'rAC
Cuts at: 669 1657 1938 2568 4989 5235 669
Size: 988 281 630 2421 246 3079
Fragments arranged by size:
3079 2421 988 630 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7645
HpaI GTT'AAC
Cuts at: 5235 5235
Size: 7645
KpnI G_GTAC'C
Cuts at: 2173 4970 2173
Size: 2797 4848
MfeI C'AATT_G
Cuts at: 5244 5244
Size: 7645
MluI A'CGCG_T
Cuts at: 7521 7521
Size: 7645
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4234 4261 6759 6943 1305
Size: 2929 27 2498 184 2007
Fragments arranged by size:
2929 2498 2007 184 27
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2702 2792 3848 4136
Size: 54 1425 343 177 693 90 1056 288
Cuts at: 4136 10
Size: 3519
Fragments arranged by size:
3519 1425 1056 693 343 288 177 90
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2745 3119 5603 5962 6121 518
Size: 648 1579 374 2484 359 159 2042
Fragments arranged by size:
2484 2042 1579 648 374 359 159
MspA1I CmG'CkG
Cuts at: 1606 2015 2097 2217 2308 2479 2961 3198 4149
Size: 409 82 120 91 171 482 237 951
Cuts at: 4149 4429 4835 5061 5738 6886 7131 1606
Size: 280 406 226 677 1148 245 2120
Fragments arranged by size:
2120 1148 951 677 482 409 406 280
245 237 226 171 120 91 82
NaeI GCC'GGC
Cuts at: 2284 2284
Size: 7645
NcoI C'CATG_G
Cuts at: 513 1065 2004 2743 513
Size: 552 939 739 5415
Fragments arranged by size:
5415 939 739 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7645
NgoMIV G'CCGG_C
Cuts at: 2282 2282
Size: 7645
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7645
NotI GC'GGCC_GC
Cuts at: 5092 5092
Size: 7645
NsiI A_TGCA'T
Cuts at: 3737 3737
Size: 7645
NspI r_CATG'y
Cuts at: 1132 1229 3076 3418 3735 5003 6548 1132
Size: 97 1847 342 317 1268 1545 2229
Fragments arranged by size:
2229 1847 1545 1268 342 317 97
PasI CC'CwG_GG
Cuts at: 1348 3865 1348
Size: 2517 5128
PciI A'CATG_T
Cuts at: 3072 3414 6544 3072
Size: 342 3130 4173
Fragments arranged by size:
4173 3130 342
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2626 2744 3865 4149 1108
Size: 1518 118 1121 284 4604
Fragments arranged by size:
4604 1518 1121 284 118
PfoI T'CCnGG_A
Cuts at: 3150 3150
Size: 7645
PmeI GTTT'AAAC
Cuts at: 4949 4949
Size: 7645
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5714 5746 7227 7259 5714
Size: 32 1481 32 6100
Fragments arranged by size:
6100 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7645
PsiI TTA'TAA
Cuts at: 5215 5215
Size: 7645
PspOMI G'GGCC_C
Cuts at: 2310 2310
Size: 7645
PstI C_TGCA'G
Cuts at: 830 2940 2960 3060 4428 4997 830
Size: 2110 20 100 1368 569 3478
Fragments arranged by size:
3478 2110 1368 569 100 20
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7645
PvuII CAG'CTG
Cuts at: 2479 2961 3198 4149 4429 4835 2479
Size: 482 237 951 280 406 5289
Fragments arranged by size:
5289 951 482 406 280 237
SacI G_AGCT'C
Cuts at: 2405 3336 3920 2405
Size: 931 584 6130
Fragments arranged by size:
6130 931 584
SacII CC_GC'GG
Cuts at: 2016 2098 2309 2016
Size: 82 211 7352
Fragments arranged by size:
7352 211 82
SalI G'TCGA_C
Cuts at: 1936 4987 1936
Size: 3051 4594
SapI GCTCTTCn'nnn_
Cuts at: 2882 3119 3428 2882
Size: 237 309 7099
Fragments arranged by size:
7099 309 237
SbfI CC_TGCA'GG
Cuts at: 2940 4997 2940
Size: 2057 5588
ScaI AGT'ACT
Cuts at: 2261 4023 5893 7605 2261
Size: 1762 1870 1712 2301
Fragments arranged by size:
2301 1870 1762 1712
SexAI A'CCwGG_T
Cuts at: 4050 4050
Size: 7645
SfcI C'TryA_G
Cuts at: 826 1045 2347 2936 2956 3056 3770 4424 4993
Size: 219 1302 589 20 100 714 654 569
Cuts at: 4993 6128 6809 7000 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1302 1135 714 681 654 589 569
219 191 100 20
SfiI GGCCn_nnn'nGGCC
Cuts at: 2288 2288
Size: 7645
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7645
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7645
SmaI CCC'GGG
Cuts at: 1872 4972 1872
Size: 3100 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5749 6650 6912 820
Size: 197 926 3806 901 262 1553
Fragments arranged by size:
3806 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5436 493
Size: 4943 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7645
SphI G_CATG'C
Cuts at: 1132 3735 5003 1132
Size: 2603 1268 3774
Fragments arranged by size:
3774 2603 1268
SspI AAT'ATT
Cuts at: 5 52 5569 5
Size: 47 5517 2081
Fragments arranged by size:
5517 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7645
StyI C'CwwG_G
Cuts at: 513 1065 2004 2605 2743 3327 3636 4470 5084
Size: 552 939 601 138 584 309 834 614
Cuts at: 5084 513
Size: 3074
Fragments arranged by size:
3074 939 834 614 601 584 552 309
138
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3291 4112 4424 5672 5689 5842 6027
Size: 1255 1711 821 312 1248 17 153 185
Cuts at: 6027 6472 325
Size: 445 1498
Fragments arranged by size:
1711 1498 1255 1248 821 445 312 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2259 3408 3544
Size: 274 80 33 51 1443 282 1149 136
Cuts at: 3544 4021 4237 5891 7603 96
Size: 477 216 1654 1712 138
Fragments arranged by size:
1712 1654 1443 1149 477 282 274 216
138 136 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3146 3178 7605 7637 3146
Size: 32 4427 32 3154
Fragments arranged by size:
4427 3154 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3721 1316
Size: 2405 5240
XbaI T'CTAG_A
Cuts at: 4111 4981 4111
Size: 870 6775
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2741 3334 2741
Size: 593 7052
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7645
XmaI C'CCGG_G
Cuts at: 1870 4970 1870
Size: 3100 4545
XmnI GAAnn'nnTTC
Cuts at: 3984 5774 3984
Size: 1790 5855
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5453 276
Size: 53 83 186 936 3919 2468
Fragments arranged by size:
3919 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BsiWI
BstZ17I Bsu36I EcoRV FseI FspAI KasI NarI NruI
PacI PmlI PpuMI PspXI PsrI RsrII SanDI SfoI
SrfI SwaI