Restriction map for
pFN21AA1378
(Circular) (Six-base) MAPSORT of: pFN21AA1378.seq Check: 5081 from: 1 to: 6565
pFN21AA1378 6565 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4375 278
Size: 53 83 186 936 2839 2468
Fragments arranged by size:
2839 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3908 1937
Size: 1971 4594
Acc65I G'GTAC_C
Cuts at: 3886 3886
Size: 6565
AclI AA'CG_TT
Cuts at: 1563 4692 5065 1563
Size: 3129 373 3063
Fragments arranged by size:
3129 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 1995 2328 2616 4628 6012 6280 1632
Size: 363 333 288 2012 1384 268 1917
Fragments arranged by size:
2012 1917 1384 363 333 288 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6368
AflIII A'CryG_T
Cuts at: 1203 3076 5464 6441 1203
Size: 1873 2388 977 1327
Fragments arranged by size:
2388 1873 1327 977
AgeI A'CCGG_T
Cuts at: 5396 5396
Size: 6565
AhdI GACnn_n'nnGTC
Cuts at: 5294 5294
Size: 6565
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6565
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6565
AlwNI CAG_nnn'CTG
Cuts at: 2909 4308 5880 2909
Size: 1399 1572 3594
Fragments arranged by size:
3594 1572 1399
ApaLI G'TGCA_C
Cuts at: 4622 5778 4622
Size: 1156 5409
ApoI r'AATT_y
Cuts at: 1493 3819 3874 4100 4252 6361 1493
Size: 2326 55 226 152 2109 1697
Fragments arranged by size:
2326 2109 1697 226 152 55
AseI AT'TA_AT
Cuts at: 160 1032 5119 160
Size: 872 4087 1606
Fragments arranged by size:
4087 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6565
AvaI C'yCGr_G
Cuts at: 1870 1943 3890 1870
Size: 73 1947 4545
Fragments arranged by size:
4545 1947 73
BamHI G'GATC_C
Cuts at: 3839 3895 4262 3839
Size: 56 367 6142
Fragments arranged by size:
6142 367 56
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3334 3886 5341 618
Size: 325 217 627 1547 552 1455 1842
Fragments arranged by size:
1842 1547 1455 627 552 325 217
BanII G_rGCy'C
Cuts at: 1391 3884 1391
Size: 2493 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2367 2619 928
Size: 971 468 252 4874
Fragments arranged by size:
4874 971 468 252
BbvCI CC'TCA_GC
Cuts at: 2730 2730
Size: 6565
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4756 4790 1903
Size: 34 2819 34 3678
Fragments arranged by size:
3678 2819 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4457 5667 4457
Size: 1210 5355
BclI T'GATC_A
Cuts at: 1548 3316 1548
Size: 1768 4797
BfrBI ATG'CAT
Cuts at: 3701 3701
Size: 6565
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5176 136
Size: 107 122 71 631 605 3504 1525
Fragments arranged by size:
3504 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6560 6560
Size: 6565
BlpI GC'TnA_GC
Cuts at: 3982 3982
Size: 6565
Bme1580I G_kGCm'C
Cuts at: 1790 4626 5782 1790
Size: 2836 1156 2573
Fragments arranged by size:
2836 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6565
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4296 5254 442
Size: 416 53 83 399 24 2879 958 1753
Fragments arranged by size:
2879 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6565
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2644 3801 5225 1239
Size: 1405 1157 1424 2579
Fragments arranged by size:
2579 1424 1405 1157
Bpu10I CC'TnA_GC
Cuts at: 2730 2730
Size: 6565
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2201 2911 4690 5555 5853 2201
Size: 710 1779 865 298 2913
Fragments arranged by size:
2913 1779 865 710 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5228 882
Size: 617 3729 2219
Fragments arranged by size:
3729 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3535 4356 493
Size: 711 2331 821 2702
Fragments arranged by size:
2702 2331 821 711
BsaBI GATnn'nnATC
Cuts at: 3450 6559 3450
Size: 3109 3456
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4372 4754 5389 275
Size: 53 83 186 936 2839 382 635 1451
Fragments arranged by size:
2839 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4998 5396 5670 5817 6228 4998
Size: 398 274 147 411 5335
Fragments arranged by size:
5335 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3774 3780 3804 3810 1178
Size: 30 165 30 2371 6 24 6 3933
Fragments arranged by size:
3933 2371 165 30 30 24 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3372 3552 1186
Size: 2186 180 4199
Fragments arranged by size:
4199 2186 180
BseYI C'CCAG_C
Cuts at: 5768 6264 5768
Size: 496 6069
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5992
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4015 4776 5804 1540
Size: 275 186 2014 761 1028 2301
Fragments arranged by size:
2301 2014 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4626 4711 5782 4626
Size: 85 1071 5409
Fragments arranged by size:
5409 1071 85
BsmI GAATG_Cn'
Cuts at: 3020 4076 4169 3020
Size: 1056 93 5416
Fragments arranged by size:
5416 1056 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3884 4626 4711 5782 1391
Size: 399 2094 742 85 1071 2174
Fragments arranged by size:
2174 2094 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6228 6228
Size: 6565
BspHI T'CATG_A
Cuts at: 4454 6184 4454
Size: 1730 4835
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2437 2680 3920 844
Size: 1593 243 1240 3489
Fragments arranged by size:
3489 1593 1240 243
BsrBI CCG'CTC
Cuts at: 1394 1823 3580 4452 1394
Size: 429 1757 872 3507
Fragments arranged by size:
3507 1757 872 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3412 3508 3703 5060 5234 65
Size: 1183 2164 96 195 1357 174 1396
Fragments arranged by size:
2164 1396 1357 1183 195 174 96
BsrFI r'CCGG_y
Cuts at: 1700 1810 2788 5209 5396 1700
Size: 110 978 2421 187 2869
Fragments arranged by size:
2869 2421 978 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6565
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6565
BssSI C'ACGA_G
Cuts at: 4625 5637 4625
Size: 1012 5553
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6565
BstBI TT'CG_AA
Cuts at: 5369 5369
Size: 6565
BstEII G'GTnAC_C
Cuts at: 1184 2993 1184
Size: 1809 4756
BstXI CCAn_nnnn'nTGG
Cuts at: 2512 2749 3850 2512
Size: 237 1101 5227
Fragments arranged by size:
5227 1101 237
BstYI r'GATC_y
Cuts at: 1972 2093 2987 3758 3839 3895 4262 4647 4664
Size: 121 894 771 81 56 367 385 17
Cuts at: 4664 6116 6202 6214 6560 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 894 771 385 367 346 121
86 81 56 17 12
Bsu36I CC'TnA_GG
Cuts at: 3235 3235
Size: 6565
BtgI C'CryG_G
Cuts at: 513 1065 2004 3352 513
Size: 552 939 1348 3726
Fragments arranged by size:
3726 1348 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5826
Fragments arranged by size:
5826 683 56
BtsI GCAGTG_nn'
Cuts at: 3769 4081 4875 4895 3769
Size: 312 794 20 5439
Fragments arranged by size:
5439 794 312 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6565
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6530
DraI TTT'AAA
Cuts at: 3869 3869
Size: 6565
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5572 809
Size: 507 4256 1802
Fragments arranged by size:
4256 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4012 4901 6530
Size: 54 1425 50 275 18 2182 889 1629
Cuts at: 6530 8
Size: 43
Fragments arranged by size:
2182 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4012 1537
Size: 275 2200 4090
Fragments arranged by size:
4090 2200 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2178 3487 4495 1353
Size: 825 1309 1008 3423
Fragments arranged by size:
3423 1309 1008 825
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2582 5152 5526 5672 387
Size: 1064 138 993 2570 374 146 1280
Fragments arranged by size:
2570 1280 1064 993 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1995 2328 2616 2644 3801 4628 5225
Size: 393 363 333 288 28 1157 827 597
Cuts at: 5225 6012 6280 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1157 827 787 597 393 363 333
288 268 28
EcoRI G'AATT_C
Cuts at: 3874 4252 3874
Size: 378 6187
FspI TGC'GCA
Cuts at: 5071 5071
Size: 6565
HaeII r_GCGC'y
Cuts at: 1678 5712 1678
Size: 4034 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3148 3180 3296 3328 3430
Size: 32 135 32 1576 32 116 32 102
Cuts at: 3430 3462 3633 3665 5206 5238 5280 5312 1373
Size: 32 171 32 1541 32 42 32 2626
Fragments arranged by size:
2626 1576 1541 171 135 116 102 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2407 3856 3909 4155 669
Size: 988 281 469 1449 53 246 3079
Fragments arranged by size:
3079 1449 988 469 281 246 53
HindIII A'AGCT_T
Cuts at: 748 2148 2648 3515 748
Size: 1400 500 867 3798
Fragments arranged by size:
3798 1400 867 500
HpaI GTT'AAC
Cuts at: 4155 4155
Size: 6565
KpnI G_GTAC'C
Cuts at: 3890 3890
Size: 6565
MfeI C'AATT_G
Cuts at: 2506 3255 4164 2506
Size: 749 909 4907
Fragments arranged by size:
4907 909 749
MluI A'CGCG_T
Cuts at: 6441 6441
Size: 6565
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2730 3736 5679 5863 1305
Size: 1425 1006 1943 184 2007
Fragments arranged by size:
2007 1943 1425 1006 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4743
Fragments arranged by size:
4743 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3081 3390 4523 4882 5041 518
Size: 648 1915 309 1133 359 159 2042
Fragments arranged by size:
2042 1915 1133 648 359 309 159
MspA1I CmG'CkG
Cuts at: 1606 3981 4658 5806 6051 1606
Size: 2375 677 1148 245 2120
Fragments arranged by size:
2375 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5074
Fragments arranged by size:
5074 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6565
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6565
NotI GC'GGCC_GC
Cuts at: 4012 4012
Size: 6565
NsiI A_TGCA'T
Cuts at: 3703 3703
Size: 6565
NspI r_CATG'y
Cuts at: 1132 1229 3080 3923 5468 1132
Size: 97 1851 843 1545 2229
Fragments arranged by size:
2229 1851 1545 843 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6565
PciI A'CATG_T
Cuts at: 3076 5464 3076
Size: 2388 4177
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2712 1108
Size: 1604 4961
PmeI GTTT'AAAC
Cuts at: 3869 3869
Size: 6565
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2081 2113 4634 4666 6147 6179 2081
Size: 32 2521 32 1481 32 2467
Fragments arranged by size:
2521 2467 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6565
PsiI TTA'TAA
Cuts at: 4135 4135
Size: 6565
PstI C_TGCA'G
Cuts at: 830 2448 3776 3917 830
Size: 1618 1328 141 3478
Fragments arranged by size:
3478 1618 1328 141
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6565
SalI G'TCGA_C
Cuts at: 1936 3907 1936
Size: 1971 4594
SapI GCTCTTCn'nnn_
Cuts at: 3487 3487
Size: 6565
SbfI CC_TGCA'GG
Cuts at: 3917 3917
Size: 6565
ScaI AGT'ACT
Cuts at: 4813 6525 4813
Size: 1712 4853
SfcI C'TryA_G
Cuts at: 826 1045 2444 3772 3913 5048 5729 5920 826
Size: 219 1399 1328 141 1135 681 191 1471
Fragments arranged by size:
1471 1399 1328 1135 681 219 191 141
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6565
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6565
SmaI CCC'GGG
Cuts at: 1872 3892 1872
Size: 2020 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2216 2890 4669 5570 5832 820
Size: 197 926 273 674 1779 901 262 1553
Fragments arranged by size:
1779 1553 926 901 674 273 262 197
SnaBI TAC'GTA
Cuts at: 493 3535 4356 493
Size: 3042 821 2702
Fragments arranged by size:
3042 2702 821
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6565
SphI G_CATG'C
Cuts at: 1132 3923 1132
Size: 2791 3774
SspI AAT'ATT
Cuts at: 5 52 2674 4489 5
Size: 47 2622 1815 2081
Fragments arranged by size:
2622 2081 1815 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6565
StyI C'CwwG_G
Cuts at: 513 1065 2004 2939 3225 4004 513
Size: 552 939 935 286 779 3074
Fragments arranged by size:
3074 939 935 779 552 286
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2795 3071 4592 4609 4762 4947 5392
Size: 1255 1215 276 1521 17 153 185 445
Cuts at: 5392 325
Size: 1498
Fragments arranged by size:
1521 1498 1255 1215 445 276 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3326 4811 6523
Size: 274 80 33 51 1443 1349 1485 1712
Cuts at: 6523 96
Size: 138
Fragments arranged by size:
1712 1485 1443 1349 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3780 3812 6525 6557 3780
Size: 32 2713 32 3788
Fragments arranged by size:
3788 2713 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2411 1316
Size: 1095 5470
XbaI T'CTAG_A
Cuts at: 3901 3901
Size: 6565
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3640 3640
Size: 6565
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6565
XmaI C'CCGG_G
Cuts at: 1870 3890 1870
Size: 2020 4545
XmnI GAAnn'nnTTC
Cuts at: 3418 4694 3418
Size: 1276 5289
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4373 276
Size: 53 83 186 936 2839 2468
Fragments arranged by size:
2839 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI