Restriction map for
pFN21AA1375
(Circular) (Six-base) MAPSORT of: pFN21AA1375.seq Check: 5894 from: 1 to: 7309
pFN21AA1375 7309 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4186 4186
Size: 7309
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5119 278
Size: 53 83 186 936 3583 2468
Fragments arranged by size:
3583 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4652 1937
Size: 2715 4594
Acc65I G'GTAC_C
Cuts at: 4630 4630
Size: 7309
AclI AA'CG_TT
Cuts at: 1563 3427 5436 5809 1563
Size: 1864 2009 373 3063
Fragments arranged by size:
3063 2009 1864 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3472 5372 6756 7024 1632
Size: 1840 1900 1384 268 1917
Fragments arranged by size:
1917 1900 1840 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2515 3946 820
Size: 197 1498 1431 4183
Fragments arranged by size:
4183 1498 1431 197
AflIII A'CryG_T
Cuts at: 1203 6208 7185 1203
Size: 5005 977 1327
Fragments arranged by size:
5005 1327 977
AgeI A'CCGG_T
Cuts at: 6140 6140
Size: 7309
AhdI GACnn_n'nnGTC
Cuts at: 2565 6038 2565
Size: 3473 3836
AleI CACnn'nnGTG
Cuts at: 1166 3014 3563 1166
Size: 1848 549 4912
Fragments arranged by size:
4912 1848 549
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2784 1184
Size: 1600 5709
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2232 2264 2232
Size: 32 7277
AlwNI CAG_nnn'CTG
Cuts at: 2422 2552 3587 5052 6624 2422
Size: 130 1035 1465 1572 3107
Fragments arranged by size:
3107 1572 1465 1035 130
ApaLI G'TGCA_C
Cuts at: 5366 6522 5366
Size: 1156 6153
ApoI r'AATT_y
Cuts at: 1493 2246 2810 3053 3416 3733 3908 3967 4618
Size: 753 564 243 363 317 175 59 651
Cuts at: 4618 4844 4996 7105 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 753 651 564 363 317 243
226 175 152 59
AseI AT'TA_AT
Cuts at: 160 1032 5863 160
Size: 872 4831 1606
Fragments arranged by size:
4831 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7309
AvaI C'yCGr_G
Cuts at: 1870 1943 2198 4543 4634 1870
Size: 73 255 2345 91 4545
Fragments arranged by size:
4545 2345 255 91 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4166 4199 4496 4529 4166
Size: 33 297 33 6946
Fragments arranged by size:
6946 297 33 33
BamHI G'GATC_C
Cuts at: 4639 5006 4639
Size: 367 6942
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2477 4630 6085 618
Size: 325 217 627 690 2153 1455 1842
Fragments arranged by size:
2153 1842 1455 690 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2142 4309 4628 1391
Size: 751 2167 319 4072
Fragments arranged by size:
4072 2167 751 319
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4106 928
Size: 971 2207 4131
Fragments arranged by size:
4131 2207 971
BbvCI CC'TCA_GC
Cuts at: 4575 4575
Size: 7309
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5500 5534 1903
Size: 34 3563 34 3678
Fragments arranged by size:
3678 3563 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5201 6411 5201
Size: 1210 6099
BclI T'GATC_A
Cuts at: 1548 2221 3304 1548
Size: 673 1083 5553
Fragments arranged by size:
5553 1083 673
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5920 136
Size: 107 122 71 631 605 4248 1525
Fragments arranged by size:
4248 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2268 2986 3269 7304 2268
Size: 718 283 4035 2273
Fragments arranged by size:
4035 2273 718 283
BlpI GC'TnA_GC
Cuts at: 2141 4378 4726 2141
Size: 2237 348 4724
Fragments arranged by size:
4724 2237 348
Bme1580I G_kGCm'C
Cuts at: 1790 2926 5370 6526 1790
Size: 1136 2444 1156 2573
Fragments arranged by size:
2573 2444 1156 1136
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7309
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2110 5040 5998
Size: 416 53 83 399 24 693 2930 958
Cuts at: 5998 442
Size: 1753
Fragments arranged by size:
2930 1753 958 693 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2400 3194 1056
Size: 1344 794 5171
Fragments arranged by size:
5171 1344 794
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4133 4133
Size: 7309
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2529 3252 3731 4212 4314 5969 1239
Size: 1290 723 479 481 102 1655 2579
Fragments arranged by size:
2579 1655 1290 723 481 479 102
Bpu10I CC'TnA_GC
Cuts at: 2350 2392 3588 4575 2350
Size: 42 1196 987 5084
Fragments arranged by size:
5084 1196 987 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2574 3756 5434 6299 6597 2574
Size: 1182 1678 865 298 3286
Fragments arranged by size:
3286 1678 1182 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3901 5972 882
Size: 617 2402 2071 2219
Fragments arranged by size:
2402 2219 2071 617
BsaAI yAC'GTr
Cuts at: 493 1204 5100 493
Size: 711 3896 2702
Fragments arranged by size:
3896 2702 711
BsaBI GATnn'nnATC
Cuts at: 2991 7303 2991
Size: 4312 2997
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5116 5498 6133 275
Size: 53 83 186 936 3583 382 635 1451
Fragments arranged by size:
3583 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5742 6140 6414 6561 6972 5742
Size: 398 274 147 411 6079
Fragments arranged by size:
6079 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2313 2343 3225 3255 1178
Size: 30 165 30 910 30 882 30 5232
Fragments arranged by size:
5232 910 882 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2150 4209 4374 4377 1186
Size: 964 2059 165 3 4118
Fragments arranged by size:
4118 2059 964 165 3
BseYI C'CCAG_C
Cuts at: 4260 4267 4340 4551 6512 7008 4260
Size: 7 73 211 1961 496 4561
Fragments arranged by size:
4561 1961 496 211 73 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2045 2406 3533 1163
Size: 573 309 361 1127 4939
Fragments arranged by size:
4939 1127 573 361 309
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2170 2532 4759 5520 6548 1540
Size: 275 186 169 362 2227 761 1028 2301
Fragments arranged by size:
2301 2227 1028 761 362 275 186 169
BsiHKAI G_wGCw'C
Cuts at: 2142 4162 4309 5370 5455 6526 2142
Size: 2020 147 1061 85 1071 2925
Fragments arranged by size:
2925 2020 1071 1061 147 85
BsmI GAATG_Cn'
Cuts at: 3601 4820 4913 3601
Size: 1219 93 5997
Fragments arranged by size:
5997 1219 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2195 2492 2195
Size: 297 7012
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2142 2926 4162 4309 4628 5370 5455
Size: 399 352 784 1236 147 319 742 85
Cuts at: 5455 6526 1391
Size: 1071 2174
Fragments arranged by size:
2174 1236 1071 784 742 399 352 319
147 85
BspEI T'CCGG_A
Cuts at: 6972 6972
Size: 7309
BspHI T'CATG_A
Cuts at: 2962 5198 6928 2962
Size: 2236 1730 3343
Fragments arranged by size:
3343 2236 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4186 4597 4664 844
Size: 3342 411 67 3489
Fragments arranged by size:
3489 3342 411 67
BsrBI CCG'CTC
Cuts at: 1394 1823 5196 1394
Size: 429 3373 3507
Fragments arranged by size:
3507 3373 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4140 5804 5978 65
Size: 1183 2892 1664 174 1396
Fragments arranged by size:
2892 1664 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5953 6140 1700
Size: 110 4143 187 2869
Fragments arranged by size:
4143 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7309
BssHII G'CGCG_C
Cuts at: 1925 2193 1925
Size: 268 7041
BssSI C'ACGA_G
Cuts at: 2186 5369 6381 2186
Size: 3183 1012 3114
Fragments arranged by size:
3183 3114 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7309
BstBI TT'CG_AA
Cuts at: 6113 6113
Size: 7309
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7309
BstXI CCAn_nnnn'nTGG
Cuts at: 2065 3175 4459 4526 2065
Size: 1110 1284 67 4848
Fragments arranged by size:
4848 1284 1110 67
BstYI r'GATC_y
Cuts at: 1972 2268 2986 2992 3269 4639 5006 5391 5408
Size: 296 718 6 277 1370 367 385 17
Cuts at: 5408 6860 6946 6958 7304 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1370 718 385 367 346 296
277 86 17 12 6
BtgI C'CryG_G
Cuts at: 513 1065 2004 2532 4458 513
Size: 552 939 528 1926 3364
Fragments arranged by size:
3364 1926 939 552 528
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6570
Fragments arranged by size:
6570 683 56
BtsI GCAGTG_nn'
Cuts at: 3646 4084 4825 5619 5639 3646
Size: 438 741 794 20 5316
Fragments arranged by size:
5316 794 741 438 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7309
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7274
DraI TTT'AAA
Cuts at: 2607 4613 2607
Size: 2006 5303
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6316 809
Size: 507 5000 1802
Fragments arranged by size:
5000 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2055 4483 4756
Size: 54 1425 50 275 18 225 2428 273
Cuts at: 4756 5645 7274 8
Size: 889 1629 43
Fragments arranged by size:
2428 1629 1425 889 275 273 225 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4756 1537
Size: 275 2944 4090
Fragments arranged by size:
4090 2944 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2100 5239 1353
Size: 747 3139 3423
Fragments arranged by size:
3423 3139 747
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2147 5896 6270 6416 387
Size: 1064 138 558 3749 374 146 1280
Fragments arranged by size:
3749 1280 1064 558 374 146 138
EcoICRI GAG'CTC
Cuts at: 2140 4307 2140
Size: 2167 5142
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2529 3252 3472 3731 4212 4314 5372
Size: 393 897 723 220 259 481 102 1058
Cuts at: 5372 5969 6756 7024 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1058 897 787 723 597 481 393
268 259 220 102
EcoO109I rG'GnC_Cy
Cuts at: 2559 4547 2559
Size: 1988 5321
EcoRI G'AATT_C
Cuts at: 4618 4996 4618
Size: 378 6931
EcoRV GAT'ATC
Cuts at: 4430 4430
Size: 7309
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2330 3029 3815 2330
Size: 699 786 5824
Fragments arranged by size:
5824 786 699
FspI TGC'GCA
Cuts at: 5815 5815
Size: 7309
HaeII r_GCGC'y
Cuts at: 1678 4235 6456 1678
Size: 2557 2221 2531
Fragments arranged by size:
2557 2531 2221
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2430 2462 2551 2583 3289
Size: 32 135 32 858 32 89 32 706
Cuts at: 3289 3321 3710 3742 5950 5982 6024 6056 1373
Size: 32 389 32 2208 32 42 32 2626
Fragments arranged by size:
2626 2208 858 706 389 135 89 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3139 4044 4653 4899 669
Size: 988 281 1201 905 609 246 3079
Fragments arranged by size:
3079 1201 988 905 609 281 246
HindIII A'AGCT_T
Cuts at: 748 2347 748
Size: 1599 5710
HpaI GTT'AAC
Cuts at: 3139 4899 3139
Size: 1760 5549
KpnI G_GTAC'C
Cuts at: 4634 4634
Size: 7309
MfeI C'AATT_G
Cuts at: 2380 3147 3216 4908 2380
Size: 767 69 1692 4781
Fragments arranged by size:
4781 1692 767 69
MluI A'CGCG_T
Cuts at: 7185 7185
Size: 7309
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3357 4247 6423 6607 1305
Size: 2052 890 2176 184 2007
Fragments arranged by size:
2176 2052 2007 890 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2057 4485 10
Size: 54 1425 343 225 2428 2834
Fragments arranged by size:
2834 2428 1425 343 225 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2021 3014 3563 4457 5267 5626 5785
Size: 648 855 993 549 894 810 359 159
Cuts at: 5785 518
Size: 2042
Fragments arranged by size:
2042 993 894 855 810 648 549 359
159
MspA1I CmG'CkG
Cuts at: 1606 2029 2065 2176 4725 5402 6550 6795 1606
Size: 423 36 111 2549 677 1148 245 2120
Fragments arranged by size:
2549 2120 1148 677 423 245 111 36
NcoI C'CATG_G
Cuts at: 513 1065 2004 4458 513
Size: 552 939 2454 3364
Fragments arranged by size:
3364 2454 939 552
NdeI CA'TA_TG
Cuts at: 387 3982 387
Size: 3595 3714
NheI G'CTAG_C
Cuts at: 1052 2396 3190 1052
Size: 1344 794 5171
Fragments arranged by size:
5171 1344 794
NotI GC'GGCC_GC
Cuts at: 4756 4756
Size: 7309
NspI r_CATG'y
Cuts at: 1132 1229 2843 4211 4667 6212 1132
Size: 97 1614 1368 456 1545 2229
Fragments arranged by size:
2229 1614 1545 1368 456 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7309
PciI A'CATG_T
Cuts at: 6208 6208
Size: 7309
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7309
PfoI T'CCnGG_A
Cuts at: 4423 4525 4423
Size: 102 7207
PmeI GTTT'AAAC
Cuts at: 4613 4613
Size: 7309
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5378 5410 6891 6923 5378
Size: 32 1481 32 5764
Fragments arranged by size:
5764 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2559 4547 2559
Size: 1988 5321
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7309
PsiI TTA'TAA
Cuts at: 4879 4879
Size: 7309
PstI C_TGCA'G
Cuts at: 830 2668 2935 4661 830
Size: 1838 267 1726 3478
Fragments arranged by size:
3478 1838 1726 267
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7309
PvuII CAG'CTG
Cuts at: 2029 2029
Size: 7309
SacI G_AGCT'C
Cuts at: 2142 4309 2142
Size: 2167 5142
SalI G'TCGA_C
Cuts at: 1936 4651 1936
Size: 2715 4594
SanDI GG'GwC_CC
Cuts at: 2559 4547 2559
Size: 1988 5321
SapI GCTCTTCn'nnn_
Cuts at: 2100 2100
Size: 7309
SbfI CC_TGCA'GG
Cuts at: 4661 4661
Size: 7309
ScaI AGT'ACT
Cuts at: 2120 5557 7269 2120
Size: 3437 1712 2160
Fragments arranged by size:
3437 2160 1712
SfcI C'TryA_G
Cuts at: 826 1045 2664 2931 4657 5792 6473 6664 826
Size: 219 1619 267 1726 1135 681 191 1471
Fragments arranged by size:
1726 1619 1471 1135 681 267 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7309
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7309
SmaI CCC'GGG
Cuts at: 1872 4545 4636 1872
Size: 2673 91 4545
Fragments arranged by size:
4545 2673 91
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2198 2515 2589 3771 3946 5413
Size: 197 926 255 317 74 1182 175 1467
Cuts at: 5413 6314 6576 820
Size: 901 262 1553
Fragments arranged by size:
1553 1467 1182 926 901 317 262 255
197 175 74
SnaBI TAC'GTA
Cuts at: 493 5100 493
Size: 4607 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7309
SphI G_CATG'C
Cuts at: 1132 2843 4667 1132
Size: 1711 1824 3774
Fragments arranged by size:
3774 1824 1711
SspI AAT'ATT
Cuts at: 5 52 3889 5233 5
Size: 47 3837 1344 2081
Fragments arranged by size:
3837 2081 1344 47
StuI AGG'CCT
Cuts at: 1517 4276 1517
Size: 2759 4550
StyI C'CwwG_G
Cuts at: 513 1065 2004 2718 4458 4748 513
Size: 552 939 714 1740 290 3074
Fragments arranged by size:
3074 1740 939 714 552 290
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2156 5336 5353 5506 5691 6136 325
Size: 1255 576 3180 17 153 185 445 1498
Fragments arranged by size:
3180 1498 1255 576 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2118 3091 5555
Size: 274 80 33 51 1443 141 973 2464
Cuts at: 5555 7267 96
Size: 1712 138
Fragments arranged by size:
2464 1712 1443 973 274 141 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7269 7301 7269
Size: 32 7277
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7309
XbaI T'CTAG_A
Cuts at: 2989 4645 2989
Size: 1656 5653
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2471 2471
Size: 7309
XhoI C'TCGA_G
Cuts at: 1943 2198 1943
Size: 255 7054
XmaI C'CCGG_G
Cuts at: 1870 4543 4634 1870
Size: 2673 91 4545
Fragments arranged by size:
4545 2673 91
XmnI GAAnn'nnTTC
Cuts at: 5438 5438
Size: 7309
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5117 276
Size: 53 83 186 936 3583 2468
Fragments arranged by size:
3583 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SanDI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BbeI BfrBI BsiWI BstZ17I
Bsu36I DraIII EcoNI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI
RsrII SacII SexAI SfiI SfoI SrfI SwaI