Restriction map for
pFN21AA1372
(Circular) (Six-base) MAPSORT of: pFN21AA1372.seq Check: 6233 from: 1 to: 6952
pFN21AA1372 6952 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2241 4205 2241
Size: 1964 4988
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3678 4762 278
Size: 53 83 186 936 2142 1084 2468
Fragments arranged by size:
2468 2142 1084 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4295 1937
Size: 2358 4594
Acc65I G'GTAC_C
Cuts at: 3867 4273 3867
Size: 406 6546
AclI AA'CG_TT
Cuts at: 1563 5079 5452 1563
Size: 3516 373 3063
Fragments arranged by size:
3516 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2087 2549 2575 2792 5015 6399 6667 1632
Size: 455 462 26 217 2223 1384 268 1917
Fragments arranged by size:
2223 1917 1384 462 455 268 217 26
AfeI AGC'GCT
Cuts at: 3011 3011
Size: 6952
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6755
AflIII A'CryG_T
Cuts at: 1203 3452 5851 6828 1203
Size: 2249 2399 977 1327
Fragments arranged by size:
2399 2249 1327 977
AgeI A'CCGG_T
Cuts at: 5783 5783
Size: 6952
AhdI GACnn_n'nnGTC
Cuts at: 2017 5681 2017
Size: 3664 3288
AleI CACnn'nnGTG
Cuts at: 1166 3887 1166
Size: 2721 4231
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6952
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2696 2728 2696
Size: 32 6920
AlwNI CAG_nnn'CTG
Cuts at: 2434 2635 4695 6267 2434
Size: 201 2060 1572 3119
Fragments arranged by size:
3119 2060 1572 201
ApaI G_GGCC'C
Cuts at: 3990 3990
Size: 6952
ApaLI G'TGCA_C
Cuts at: 3090 3752 5009 6165 3090
Size: 662 1257 1156 3877
Fragments arranged by size:
3877 1257 1156 662
ApoI r'AATT_y
Cuts at: 1493 4261 4487 4639 6748 1493
Size: 2768 226 152 2109 1697
Fragments arranged by size:
2768 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5506 160
Size: 872 4474 1606
Fragments arranged by size:
4474 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6952
AvaI C'yCGr_G
Cuts at: 1870 1943 2290 2350 2861 2961 3066 3509 3854
Size: 73 347 60 511 100 105 443 345
Cuts at: 3854 3885 3989 4277 1870
Size: 31 104 288 4545
Fragments arranged by size:
4545 511 443 347 345 288 105 104
100 73 60 31
BamHI G'GATC_C
Cuts at: 4282 4649 4282
Size: 367 6585
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2744 3654 3867 4273 5728
Size: 325 217 627 957 910 213 406 1455
Cuts at: 5728 618
Size: 1842
Fragments arranged by size:
1842 1455 957 910 627 406 325 217
213
BanII G_rGCy'C
Cuts at: 1391 2871 3035 3470 3855 3990 4007 4029 4271
Size: 1480 164 435 385 135 17 22 242
Cuts at: 4271 1391
Size: 4072
Fragments arranged by size:
4072 1480 435 385 242 164 135 22
17
BbeI G_GCGC'C
Cuts at: 3658 3658
Size: 6952
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3060 3402 3759 4176 4240 928
Size: 971 1161 342 357 417 64 3640
Fragments arranged by size:
3640 1161 971 417 357 342 64
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2086 2120 3533 3567 5143 5177 1903
Size: 34 149 34 1413 34 1576 34 3678
Fragments arranged by size:
3678 1576 1413 149 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4844 6054 4844
Size: 1210 5742
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6952
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3030 5563 136
Size: 107 122 71 631 605 1358 2533 1525
Fragments arranged by size:
2533 1525 1358 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6947 6947
Size: 6952
BlpI GC'TnA_GC
Cuts at: 3378 3587 4369 3378
Size: 209 782 5961
Fragments arranged by size:
5961 782 209
Bme1580I G_kGCm'C
Cuts at: 1790 2816 3094 3756 3990 5013 6169 1790
Size: 1026 278 662 234 1023 1156 2573
Fragments arranged by size:
2573 1156 1026 1023 662 278 234
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6952
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2023 4159 4683
Size: 416 53 83 399 24 606 2136 524
Cuts at: 4683 5641 442
Size: 958 1753
Fragments arranged by size:
2136 1753 958 606 524 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6952
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2641 4021 2641
Size: 1380 5572
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2327 3780 4179 5612 1239
Size: 1088 1453 399 1433 2579
Fragments arranged by size:
2579 1453 1433 1088 399
Bpu10I CC'TnA_GC
Cuts at: 3006 3006
Size: 6952
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2171 2819 5077 5942 6240 2171
Size: 648 2258 865 298 2883
Fragments arranged by size:
2883 2258 865 648 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2528 5615 882
Size: 617 1029 3087 2219
Fragments arranged by size:
3087 2219 1029 617
BsaAI yAC'GTr
Cuts at: 493 1204 4743 493
Size: 711 3539 2702
Fragments arranged by size:
3539 2702 711
BsaBI GATnn'nnATC
Cuts at: 6946 6946
Size: 6952
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2582 2726 3655 3675
Size: 53 83 186 936 1049 144 929 20
Cuts at: 3675 4759 5141 5776 275
Size: 1084 382 635 1451
Fragments arranged by size:
1451 1084 1049 936 929 635 382 186
144 83 53 20
BsaWI w'CCGG_w
Cuts at: 2343 4017 5385 5783 6057 6204 6615 2343
Size: 1674 1368 398 274 147 411 2680
Fragments arranged by size:
2680 1674 1368 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2696 2726 2942 2972 3911
Size: 30 165 30 1293 30 216 30 939
Cuts at: 3911 3941 1178
Size: 30 4189
Fragments arranged by size:
4189 1293 939 216 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2228 2816 2879 2947 2981 2991 3266 3296
Size: 1042 588 63 68 34 10 275 30
Cuts at: 3296 3299 3338 3341 1186
Size: 3 39 3 4797
Fragments arranged by size:
4797 1042 588 275 68 63 39 34
30 10 3 3
BseYI C'CCAG_C
Cuts at: 2434 2748 6155 6651 2434
Size: 314 3407 496 2735
Fragments arranged by size:
3407 2735 496 314
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2777 4019 4113 1163
Size: 573 1041 1242 94 4002
Fragments arranged by size:
4002 1242 1041 573 94
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4402 5163 6191 1540
Size: 275 186 2401 761 1028 2301
Fragments arranged by size:
2401 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2187 2607 3094 3756 5013 5098 6169 2187
Size: 420 487 662 1257 85 1071 2970
Fragments arranged by size:
2970 1257 1071 662 487 420 85
BsmI GAATG_Cn'
Cuts at: 4463 4556 4463
Size: 93 6859
BsmBI CGTCTCn'nnnn_
Cuts at: 2959 3682 2959
Size: 723 6229
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2187 2607 2816 2871 3035 3094 3470
Size: 399 397 420 209 55 164 59 376
Cuts at: 3470 3756 3855 3990 4007 4029 4271 5013 5098
Size: 286 99 135 17 22 242 742 85
Cuts at: 5098 6169 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 420 399 397 376 286
242 209 164 135 99 85 59 55
22 17
BspEI T'CCGG_A
Cuts at: 4017 6615 4017
Size: 2598 4354
BspHI T'CATG_A
Cuts at: 4841 6571 4841
Size: 1730 5222
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2241 2787 3648 4205 4307 844
Size: 1397 546 861 557 102 3489
Fragments arranged by size:
3489 1397 861 557 546 102
BsrBI CCG'CTC
Cuts at: 1394 1823 3410 3448 3474 4839 1394
Size: 429 1587 38 26 1365 3507
Fragments arranged by size:
3507 1587 1365 429 38 26
BsrDI GCAATG_nn'
Cuts at: 65 1248 2055 4228 5447 5621 65
Size: 1183 807 2173 1219 174 1396
Fragments arranged by size:
2173 1396 1219 1183 807 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3966 5596 5783 1700
Size: 110 2156 1630 187 2869
Fragments arranged by size:
2869 2156 1630 187 110
BsrGI T'GTAC_A
Cuts at: 96 3168 96
Size: 3072 3880
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6952
BssSI C'ACGA_G
Cuts at: 5012 6024 5012
Size: 1012 5940
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2544 794
Size: 1750 5202
BstBI TT'CG_AA
Cuts at: 3906 5756 3906
Size: 1850 5102
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6952
BstXI CCAn_nnnn'nTGG
Cuts at: 2305 2305
Size: 6952
BstYI r'GATC_y
Cuts at: 1972 2362 2820 2928 3663 4282 4649 5034 5051
Size: 390 458 108 735 619 367 385 17
Cuts at: 5051 6503 6589 6601 6947 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 735 619 458 390 385 367
346 108 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3939 3939
Size: 6952
BtgI C'CryG_G
Cuts at: 513 1065 2256 3718 513
Size: 552 1191 1462 3747
Fragments arranged by size:
3747 1462 1191 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6213
Fragments arranged by size:
6213 683 56
BtsI GCAGTG_nn'
Cuts at: 2060 2329 2657 3133 3617 3748 4468 5262 5282
Size: 269 328 476 484 131 720 794 20
Cuts at: 5282 2060
Size: 3730
Fragments arranged by size:
3730 794 720 484 476 328 269 131
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6952
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2874 2909 529
Size: 35 2310 35 4572
Fragments arranged by size:
4572 2310 35 35
DraI TTT'AAA
Cuts at: 4256 4256
Size: 6952
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5959 809
Size: 507 4643 1802
Fragments arranged by size:
4643 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2496 3715 3721
Size: 54 1425 50 275 18 666 1219 6
Cuts at: 3721 4399 5288 6917 8
Size: 678 889 1629 43
Fragments arranged by size:
1629 1425 1219 889 678 666 275 54
50 43 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 4399 1537
Size: 275 2587 4090
Fragments arranged by size:
4090 2587 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2456 2793 3330 3339 3904 4012 4882 1353
Size: 1103 337 537 9 565 108 870 3423
Fragments arranged by size:
3423 1103 870 565 537 337 108 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2333 2847 3686 5539 5913 6059
Size: 1064 138 744 514 839 1853 374 146
Cuts at: 6059 387
Size: 1280
Fragments arranged by size:
1853 1280 1064 839 744 514 374 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2087 2327 2549 2575 2792 3780 4179
Size: 393 455 240 222 26 217 988 399
Cuts at: 4179 5015 5612 6399 6667 1239
Size: 836 597 787 268 1524
Fragments arranged by size:
1524 988 836 787 597 455 399 393
268 240 222 217 26
EcoNI CCTnn'n_nnAGG
Cuts at: 4037 4037
Size: 6952
EcoO109I rG'GnC_Cy
Cuts at: 2071 2395 3113 3414 3986 2071
Size: 324 718 301 572 5037
Fragments arranged by size:
5037 718 572 324 301
EcoRI G'AATT_C
Cuts at: 4261 4639 4261
Size: 378 6574
FspI TGC'GCA
Cuts at: 3644 5458 3644
Size: 1814 5138
HaeII r_GCGC'y
Cuts at: 1678 3013 3658 3810 6099 1678
Size: 1335 645 152 2289 2531
Fragments arranged by size:
2531 2289 1335 645 152
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2003 2035 2273 2305 2908
Size: 32 135 32 431 32 238 32 603
Cuts at: 2908 2920 2940 2952 3662 3694 4175 4207 5593
Size: 12 20 12 710 32 481 32 1386
Cuts at: 5593 5625 5667 5699 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1386 710 603 481 431 238 135
42 32 32 32 32 32 32 32
32 20 12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 2554 3493 4296 4542 669
Size: 988 281 616 939 803 246 3079
Fragments arranged by size:
3079 988 939 803 616 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6952
HpaI GTT'AAC
Cuts at: 3493 4542 3493
Size: 1049 5903
KasI G'GCGC_C
Cuts at: 3654 3654
Size: 6952
KpnI G_GTAC'C
Cuts at: 3871 4277 3871
Size: 406 6546
MfeI C'AATT_G
Cuts at: 4551 4551
Size: 6952
MluI A'CGCG_T
Cuts at: 6828 6828
Size: 6952
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6066 6250 1305
Size: 4761 184 2007
Fragments arranged by size:
4761 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2498 3723 10
Size: 54 1425 343 666 1225 3239
Fragments arranged by size:
3239 1425 1225 666 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3423 3887 4910 5269 5428 518
Size: 648 2257 464 1023 359 159 2042
Fragments arranged by size:
2257 2042 1023 648 464 359 159
MspA1I CmG'CkG
Cuts at: 1606 2301 2314 2431 2683 3973 4368 5045 6193
Size: 695 13 117 252 1290 395 677 1148
Cuts at: 6193 6438 1606
Size: 245 2120
Fragments arranged by size:
2120 1290 1148 695 677 395 252 245
117 13
NarI GG'CG_CC
Cuts at: 3655 3655
Size: 6952
NcoI C'CATG_G
Cuts at: 513 1065 2256 513
Size: 552 1191 5209
Fragments arranged by size:
5209 1191 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6952
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6952
NotI GC'GGCC_GC
Cuts at: 4399 4399
Size: 6952
NspI r_CATG'y
Cuts at: 1132 1229 2197 2547 3456 4310 5855 1132
Size: 97 968 350 909 854 1545 2229
Fragments arranged by size:
2229 1545 968 909 854 350 97
PasI CC'CwG_GG
Cuts at: 1348 2029 3978 1348
Size: 681 1949 4322
Fragments arranged by size:
4322 1949 681
PciI A'CATG_T
Cuts at: 3452 5851 3452
Size: 2399 4553
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2335 3710 4243 1108
Size: 1227 1375 533 3817
Fragments arranged by size:
3817 1375 1227 533
PfoI T'CCnGG_A
Cuts at: 2358 2720 3480 3666 2358
Size: 362 760 186 5644
Fragments arranged by size:
5644 760 362 186
PmeI GTTT'AAAC
Cuts at: 4256 4256
Size: 6952
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2696 2728 5021 5053 6534 6566 2696
Size: 32 2293 32 1481 32 3082
Fragments arranged by size:
3082 2293 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2395 3113 3414 2395
Size: 718 301 5933
Fragments arranged by size:
5933 718 301
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6952
PsiI TTA'TAA
Cuts at: 4522 4522
Size: 6952
PspOMI G'GGCC_C
Cuts at: 3986 3986
Size: 6952
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3851 3883 3851
Size: 32 6920
PstI C_TGCA'G
Cuts at: 830 2652 3564 3743 4216 4304 830
Size: 1822 912 179 473 88 3478
Fragments arranged by size:
3478 1822 912 473 179 88
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6952
PvuII CAG'CTG
Cuts at: 2301 2314 2431 2301
Size: 13 117 6822
Fragments arranged by size:
6822 117 13
SalI G'TCGA_C
Cuts at: 1936 4294 1936
Size: 2358 4594
SanDI GG'GwC_CC
Cuts at: 3414 3414
Size: 6952
SapI GCTCTTCn'nnn_
Cuts at: 2456 4012 2456
Size: 1556 5396
SbfI CC_TGCA'GG
Cuts at: 3564 4216 4304 3564
Size: 652 88 6212
Fragments arranged by size:
6212 652 88
ScaI AGT'ACT
Cuts at: 5200 6912 5200
Size: 1712 5240
SexAI A'CCwGG_T
Cuts at: 3486 3486
Size: 6952
SfcI C'TryA_G
Cuts at: 826 1045 2648 3560 3739 4082 4212 4300 5435
Size: 219 1603 912 179 343 130 88 1135
Cuts at: 5435 6116 6307 826
Size: 681 191 1471
Fragments arranged by size:
1603 1471 1135 912 681 343 219 191
179 130 88
SfoI GGC'GCC
Cuts at: 3656 3656
Size: 6952
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6952
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6952
SmaI CCC'GGG
Cuts at: 1872 2352 2963 3068 4279 1872
Size: 480 611 105 1211 4545
Fragments arranged by size:
4545 1211 611 480 105
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2186 2834 5056 5957 6219 820
Size: 197 926 243 648 2222 901 262 1553
Fragments arranged by size:
2222 1553 926 901 648 262 243 197
SnaBI TAC'GTA
Cuts at: 493 4743 493
Size: 4250 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6952
SphI G_CATG'C
Cuts at: 1132 4310 1132
Size: 3178 3774
SspI AAT'ATT
Cuts at: 5 52 2041 4876 5
Size: 47 1989 2835 2081
Fragments arranged by size:
2835 2081 1989 47
StuI AGG'CCT
Cuts at: 1517 2570 2972 3180 3728 1517
Size: 1053 402 208 548 4741
Fragments arranged by size:
4741 1053 548 402 208
StyI C'CwwG_G
Cuts at: 513 1065 2256 3189 3592 4391 513
Size: 552 1191 933 403 799 3074
Fragments arranged by size:
3074 1191 933 799 552 403
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2005 2762 4979 4996 5149 5334 5779
Size: 1255 425 757 2217 17 153 185 445
Cuts at: 5779 325
Size: 1498
Fragments arranged by size:
2217 1498 1255 757 445 425 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2190 3168 5198
Size: 274 80 33 51 1443 213 978 2030
Cuts at: 5198 6910 96
Size: 1712 138
Fragments arranged by size:
2030 1712 1443 978 274 213 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6912 6944 6912
Size: 32 6920
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6952
XbaI T'CTAG_A
Cuts at: 4010 4288 4010
Size: 278 6674
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6952
XmaI C'CCGG_G
Cuts at: 1870 2350 2961 3066 4277 1870
Size: 480 611 105 1211 4545
Fragments arranged by size:
4545 1211 611 480 105
XmnI GAAnn'nnTTC
Cuts at: 2135 5081 2135
Size: 2946 4006
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3676 4760 276
Size: 53 83 186 936 2142 1084 2468
Fragments arranged by size:
2468 2142 1084 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I DraIII
EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PmlI PspXI RsrII SacI SacII SfiI
SrfI SwaI XcmI