Restriction map for
pFN21AA1370
(Circular) (Six-base) MAPSORT of: pFN21AA1370.seq Check: 9809 from: 1 to: 7933
pFN21AA1370 7933 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3187 5743 278
Size: 53 83 186 936 1651 2556 2468
Fragments arranged by size:
2556 2468 1651 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2553 4316 5276 1937
Size: 616 1763 960 4594
Fragments arranged by size:
4594 1763 960 616
Acc65I G'GTAC_C
Cuts at: 5254 5254
Size: 7933
AclI AA'CG_TT
Cuts at: 1563 6060 6433 1563
Size: 4497 373 3063
Fragments arranged by size:
4497 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4007 4848 5996 7380 7648 1632
Size: 2375 841 1148 1384 268 1917
Fragments arranged by size:
2375 1917 1384 1148 841 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7736
AflIII A'CryG_T
Cuts at: 1203 3172 4142 4319 6832 7809 1203
Size: 1969 970 177 2513 977 1327
Fragments arranged by size:
2513 1969 1327 977 970 177
AgeI A'CCGG_T
Cuts at: 6764 6764
Size: 7933
AhdI GACnn_n'nnGTC
Cuts at: 4312 6662 4312
Size: 2350 5583
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7933
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7933
AlwNI CAG_nnn'CTG
Cuts at: 2803 2915 5676 7248 2803
Size: 112 2761 1572 3488
Fragments arranged by size:
3488 2761 1572 112
ApaI G_GGCC'C
Cuts at: 2319 2319
Size: 7933
ApaLI G'TGCA_C
Cuts at: 5990 7146 5990
Size: 1156 6777
ApoI r'AATT_y
Cuts at: 1493 2566 2721 3336 3589 4007 4645 5242 5468
Size: 1073 155 615 253 418 638 597 226
Cuts at: 5468 5620 7729 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1073 638 615 597 418 253
226 155 152
AseI AT'TA_AT
Cuts at: 160 1032 6487 160
Size: 872 5455 1606
Fragments arranged by size:
5455 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7933
AvaI C'yCGr_G
Cuts at: 1870 1943 5258 1870
Size: 73 3315 4545
Fragments arranged by size:
4545 3315 73
AvrII C'CTAG_G
Cuts at: 4639 4639
Size: 7933
BamHI G'GATC_C
Cuts at: 5263 5630 5263
Size: 367 7566
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2830 5254 6709 618
Size: 325 217 627 1043 2424 1455 1842
Fragments arranged by size:
2424 1842 1455 1043 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2319 5252 1391
Size: 928 2933 4072
Fragments arranged by size:
4072 2933 928
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2441 3324 3588 928
Size: 971 542 883 264 5273
Fragments arranged by size:
5273 971 883 542 264
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6124 6158 1903
Size: 34 4187 34 3678
Fragments arranged by size:
4187 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5825 7035 5825
Size: 1210 6723
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7933
BfrBI ATG'CAT
Cuts at: 2366 3535 2366
Size: 1169 6764
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6544 136
Size: 107 122 71 631 605 4872 1525
Fragments arranged by size:
4872 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5106 7928 5106
Size: 2822 5111
BlpI GC'TnA_GC
Cuts at: 3474 5350 3474
Size: 1876 6057
Bme1580I G_kGCm'C
Cuts at: 1790 2319 5994 7150 1790
Size: 529 3675 1156 2573
Fragments arranged by size:
3675 2573 1156 529
BmgBI CAC'GTC
Cuts at: 1318 2682 1318
Size: 1364 6569
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2159 2197 5664
Size: 416 53 83 399 24 742 38 3467
Cuts at: 5664 6622 442
Size: 958 1753
Fragments arranged by size:
3467 1753 958 742 416 399 83 53
38 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7933
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2517 2517
Size: 7933
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2130 3425 3526 6593 1239
Size: 891 1295 101 3067 2579
Fragments arranged by size:
3067 2579 1295 891 101
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2928 3183 6058 6923 7221 2928
Size: 255 2875 865 298 3640
Fragments arranged by size:
3640 2875 865 298 255
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2071 6596 882
Size: 617 572 4525 2219
Fragments arranged by size:
4525 2219 617 572
BsaAI yAC'GTr
Cuts at: 493 1204 3795 5724 493
Size: 711 2591 1929 2702
Fragments arranged by size:
2702 2591 1929 711
BsaBI GATnn'nnATC
Cuts at: 7927 7927
Size: 7933
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3184 5740 6122 6757
Size: 53 83 186 936 1651 2556 382 635
Cuts at: 6757 275
Size: 1451
Fragments arranged by size:
2556 1651 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6366 6764 7038 7185 7596 6366
Size: 398 274 147 411 6703
Fragments arranged by size:
6703 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7708
Fragments arranged by size:
7708 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2069 3348 1186
Size: 883 1279 5771
Fragments arranged by size:
5771 1279 883
BseYI C'CCAG_C
Cuts at: 7136 7632 7136
Size: 496 7437
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2704 1163
Size: 573 968 6392
Fragments arranged by size:
6392 968 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5383 6144 7172 1540
Size: 275 186 3382 761 1028 2301
Fragments arranged by size:
3382 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3489 4615 5994 6079 7150 3489
Size: 1126 1379 85 1071 4272
Fragments arranged by size:
4272 1379 1126 1071 85
BsiWI C'GTAC_G
Cuts at: 5125 5125
Size: 7933
BsmI GAATG_Cn'
Cuts at: 5444 5537 5444
Size: 93 7840
BsmBI CGTCTCn'nnnn_
Cuts at: 5115 5115
Size: 7933
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2319 3489 4615 5252 5994 6079 7150
Size: 399 529 1170 1126 637 742 85 1071
Cuts at: 7150 1391
Size: 2174
Fragments arranged by size:
2174 1170 1126 1071 742 637 529 399
85
BspEI T'CCGG_A
Cuts at: 7596 7596
Size: 7933
BspHI T'CATG_A
Cuts at: 4585 5822 7552 4585
Size: 1237 1730 4966
Fragments arranged by size:
4966 1730 1237
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5288 844
Size: 4444 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2457 3112 3448 5820 1394
Size: 429 634 655 336 2372 3507
Fragments arranged by size:
3507 2372 655 634 429 336
BsrDI GCAATG_nn'
Cuts at: 65 1248 2137 3123 3913 6428 6602 65
Size: 1183 889 986 790 2515 174 1396
Fragments arranged by size:
2515 1396 1183 986 889 790 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6577 6764 1700
Size: 110 4767 187 2869
Fragments arranged by size:
4767 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3959 96
Size: 3863 4070
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7933
BssSI C'ACGA_G
Cuts at: 5993 7005 5993
Size: 1012 6921
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3134 3470 4727 794
Size: 2340 336 1257 4000
Fragments arranged by size:
4000 2340 1257 336
BstBI TT'CG_AA
Cuts at: 6737 6737
Size: 7933
BstEII G'GTnAC_C
Cuts at: 1184 2414 1184
Size: 1230 6703
BstYI r'GATC_y
Cuts at: 1972 2385 4255 5106 5263 5630 6015 6032 7484
Size: 413 1870 851 157 367 385 17 1452
Cuts at: 7484 7570 7582 7928 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1870 1452 851 413 385 367 346
157 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2826 2932 513
Size: 552 1761 106 5514
Fragments arranged by size:
5514 1761 552 106
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2322 487
Size: 683 56 1096 6098
Fragments arranged by size:
6098 1096 683 56
BtsI GCAGTG_nn'
Cuts at: 5449 6243 6263 5449
Size: 794 20 7119
Fragments arranged by size:
7119 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7933
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2253 2288 529
Size: 35 1689 35 6174
Fragments arranged by size:
6174 1689 35 35
DraI TTT'AAA
Cuts at: 3012 3709 5237 3012
Size: 697 1528 5708
Fragments arranged by size:
5708 1528 697
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6940 809
Size: 507 5624 1802
Fragments arranged by size:
5624 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5380 6269 7898
Size: 54 1425 50 275 18 3550 889 1629
Cuts at: 7898 8
Size: 43
Fragments arranged by size:
3550 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5380 1537
Size: 275 3568 4090
Fragments arranged by size:
4090 3568 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2424 4455 4758 5006 5863 1353
Size: 1071 2031 303 248 857 3423
Fragments arranged by size:
3423 2031 1071 857 303 248
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6520 6894 7040 387
Size: 1064 138 4931 374 146 1280
Fragments arranged by size:
4931 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2130 3425 3526 4007 4848 5996 6593
Size: 393 498 1295 101 481 841 1148 597
Cuts at: 6593 7380 7648 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1295 1148 841 787 597 498 481
393 268 101
EcoNI CCTnn'n_nnAGG
Cuts at: 3090 3426 4863 3090
Size: 336 1437 6160
Fragments arranged by size:
6160 1437 336
EcoRI G'AATT_C
Cuts at: 2721 5242 5620 2721
Size: 2521 378 5034
Fragments arranged by size:
5034 2521 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3387 4746 3387
Size: 1359 6574
FspI TGC'GCA
Cuts at: 6439 6439
Size: 7933
HaeII r_GCGC'y
Cuts at: 1678 7080 1678
Size: 5402 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2969 3001 3602 3634 4298
Size: 32 135 32 1397 32 601 32 664
Cuts at: 4298 4330 6574 6606 6648 6680 1373
Size: 32 2244 32 42 32 2626
Fragments arranged by size:
2626 2244 1397 664 601 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5277 5523 669
Size: 988 281 3339 246 3079
Fragments arranged by size:
3339 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2131 3273 4528 748
Size: 1383 1142 1255 4153
Fragments arranged by size:
4153 1383 1255 1142
HpaI GTT'AAC
Cuts at: 5523 5523
Size: 7933
KpnI G_GTAC'C
Cuts at: 5258 5258
Size: 7933
MfeI C'AATT_G
Cuts at: 2583 5532 2583
Size: 2949 4984
MluI A'CGCG_T
Cuts at: 7809 7809
Size: 7933
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3388 3521 4053 4283 5062 7047 7231 1305
Size: 2083 133 532 230 779 1985 184 2007
Fragments arranged by size:
2083 2007 1985 779 532 230 184 133
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6111
Fragments arranged by size:
6111 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2104 2245 4371 5227 5891 6250 6409
Size: 648 938 141 2126 856 664 359 159
Cuts at: 6409 518
Size: 2042
Fragments arranged by size:
2126 2042 938 856 664 648 359 159
141
MspA1I CmG'CkG
Cuts at: 1606 2885 3071 5349 6026 7174 7419 1606
Size: 1279 186 2278 677 1148 245 2120
Fragments arranged by size:
2278 2120 1279 1148 677 245 186
NcoI C'CATG_G
Cuts at: 513 1065 2826 2932 513
Size: 552 1761 106 5514
Fragments arranged by size:
5514 1761 552 106
NdeI CA'TA_TG
Cuts at: 387 3399 3577 5176 387
Size: 3012 178 1599 3144
Fragments arranged by size:
3144 3012 1599 178
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7933
NotI GC'GGCC_GC
Cuts at: 5380 5380
Size: 7933
NsiI A_TGCA'T
Cuts at: 2368 3537 2368
Size: 1169 6764
NspI r_CATG'y
Cuts at: 1132 1229 2594 2852 3176 3535 4111 4146 4323
Size: 97 1365 258 324 359 576 35 177
Cuts at: 4323 5291 6836 1132
Size: 968 1545 2229
Fragments arranged by size:
2229 1545 1365 968 576 359 324 258
177 97 35
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7933
PciI A'CATG_T
Cuts at: 3172 4142 4319 6832 3172
Size: 970 177 2513 4273
Fragments arranged by size:
4273 2513 970 177
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2915 1108
Size: 1807 6126
PfoI T'CCnGG_A
Cuts at: 3441 3441
Size: 7933
PmeI GTTT'AAAC
Cuts at: 5237 5237
Size: 7933
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6002 6034 7515 7547 6002
Size: 32 1481 32 6388
Fragments arranged by size:
6388 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7933
PsiI TTA'TAA
Cuts at: 5503 5503
Size: 7933
PspOMI G'GGCC_C
Cuts at: 2315 2315
Size: 7933
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2776 2808 5043 5075 2776
Size: 32 2235 32 5634
Fragments arranged by size:
5634 2235 32 32
PstI C_TGCA'G
Cuts at: 830 2884 5285 830
Size: 2054 2401 3478
Fragments arranged by size:
3478 2401 2054
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7933
PvuII CAG'CTG
Cuts at: 2885 3071 2885
Size: 186 7747
SalI G'TCGA_C
Cuts at: 1936 5275 1936
Size: 3339 4594
SbfI CC_TGCA'GG
Cuts at: 5285 5285
Size: 7933
ScaI AGT'ACT
Cuts at: 6181 7893 6181
Size: 1712 6221
SfcI C'TryA_G
Cuts at: 826 1045 2302 2530 2558 2880 4774 5281 6416
Size: 219 1257 228 28 322 1894 507 1135
Cuts at: 6416 7097 7288 826
Size: 681 191 1471
Fragments arranged by size:
1894 1471 1257 1135 681 507 322 228
219 191 28
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7933
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7933
SmaI CCC'GGG
Cuts at: 1872 5260 1872
Size: 3388 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2943 3198 6037 6938 7200 820
Size: 197 926 1000 255 2839 901 262 1553
Fragments arranged by size:
2839 1553 1000 926 901 262 255 197
SnaBI TAC'GTA
Cuts at: 493 3795 5724 493
Size: 3302 1929 2702
Fragments arranged by size:
3302 2702 1929
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7933
SphI G_CATG'C
Cuts at: 1132 5291 1132
Size: 4159 3774
SspI AAT'ATT
Cuts at: 5 52 2036 2524 2701 2953 4088 5857 5
Size: 47 1984 488 177 252 1135 1769 2081
Fragments arranged by size:
2081 1984 1769 1135 488 252 177 47
StuI AGG'CCT
Cuts at: 1517 4135 1517
Size: 2618 5315
StyI C'CwwG_G
Cuts at: 513 1065 2826 2932 4401 4639 4834 5372 513
Size: 552 1761 106 1469 238 195 538 3074
Fragments arranged by size:
3074 1761 1469 552 538 238 195 106
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2031 2433 4802 5960 5977 6130 6315
Size: 1255 451 402 2369 1158 17 153 185
Cuts at: 6315 6760 325
Size: 445 1498
Fragments arranged by size:
2369 1498 1255 1158 451 445 402 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3959 6179 7891
Size: 274 80 33 51 1443 1982 2220 1712
Cuts at: 7891 96
Size: 138
Fragments arranged by size:
2220 1982 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7893 7925 7893
Size: 32 7901
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7933
XbaI T'CTAG_A
Cuts at: 4538 5141 5269 4538
Size: 603 128 7202
Fragments arranged by size:
7202 603 128
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7933
XmaI C'CCGG_G
Cuts at: 1870 5258 1870
Size: 3388 4545
XmnI GAAnn'nnTTC
Cuts at: 2133 4529 4984 6062 2133
Size: 2396 455 1078 4004
Fragments arranged by size:
4004 2396 1078 455
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3185 5741 276
Size: 53 83 186 936 1651 2556 2468
Fragments arranged by size:
2556 2468 1651 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BbvCI Bpu10I
BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PpuMI PspXI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI